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All Journal Media Kesehatan Masyarakat Indonesia Jurnal Ekonomi & Bisnis PNJ (JEKBIS PNJ) JURNAL MANAJEMEN MUTU PENDIDIKAN (JMMP) Jurnal Studi Sosial Program Pascasarjana P-IPS AL-IDARAH: JURNAL KEPENDIDIKAN ISLAM Journal of Research and Technology Jurnal Dunia Kesmas Jurnal Kreativitas PKM PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Berkala Ilmiah Kimia Farmasi Stigma : Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Jurnal Pendidikan Dasar dan Sosial Humaniora International Journal of Education and Digital Learning (IJEDL) Camellia Journal of Educational Science and E-Learning Journal of Education and Pedagogy Folia Medica Indonesiana
Sudjarwo Sudjarwo
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Journal : Journal of Research and Technology

 1 
VERIFIKASI LINIERITAS KURVA BAKU TESTOSTERON MENGGUNAKAN METODE ELISA (ENZYME-LINKED IMMUNOSORBENT ASSAY) Fernanda, MA Hanny Ferry; Sa'adi, Ashon; Sudjarwo, Sudjarwo
Journal of Research and Technology Vol 5, No 1 (2019)
Publisher : Universitas Nahdlatul Ulama Sidoarjo

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Abstract

ELISA (Enzyme-Linked Linked Immunosorbent Assay) is one of the quantitative analysis methods that is often used to determine the levels of active compounds in biological samples. In this study, we will verify the method of determining testosterone active compounds in blood and urine samples of female patients who have PCOS (Polycystic Ovary Syndrome) using the Human Testosterone ELISA Kit. Before conducting testosterone levels using ELISA, it is necessary to verify the linearity of the standard curve first to determine the effect of testosterone standard levels on the analyte response in the form of optical density. The linearity verification of the standard testosterone curve is calculated using linear regression calculations with the results of the coefficient of determination which is 0.978 with a value of α = 0.05. While for testing with the calculation of 4 parameter logistic (4PL), the results of the coefficient of determination are 0.999. Based on these results it can be concluded that the testosterone standard level has a significant influence on the response of optical density analytes.
MEMBANDINGAKAN REGRESI 4 PL DAN LINIER FIT UNTUK VERIFIKASI HORMON 17β-ESTRADIOL MENGGUNAKAN METODE ELISA Sandiya, Arroofita Ani; Sa'adi, Ashon; Sudjarwo, Sudjarwo
Journal of Research and Technology Vol 5, No 1 (2019)
Publisher : Universitas Nahdlatul Ulama Sidoarjo

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Abstract

One solution for infertile couples to get offspring is IVF, where one of the stages is a HOT procedure, at which stage there is an increase in steroid hormone levels (estrogen) as a result of the development of ovarian follicles. The 17β-estradiol hormone was chosen to be verified because it can be used as a marker or marker to show the maturity of the follicle. Linear and logistic regression are the two most commonly used in curve making models for sandwich immunoassays. Although linear regression may be useful when analyzing samples included in the linear part of the analyte response curve, logistic regression is the preferred type of regression for multiplex immunoassays. Verification of the 17β estradiol hormone regression results using linear fit obtained the result of r = 0.952 while the regression value used 4 PL to get the result r = 0.998. But the results shown in the verification of the 17β estradiol hormone are good, this is evidenced by the use of SPSS software where F = 78.712 is obtained, where the value is greater than the value of F table (6.61) which means the value of independent variable (concentration) on value of the dependent variable (optical density value). The linearity values obtained through verification using the 4PL model indicate that the method is better linearity reported based on Linear regression.
Co-Authors A Effendi Sanusi A.A. Ketut Agung Cahyawan W Acepudin Acepudin Achmad Fuad Hafid Achmad Toto Poernomo Adei Saputra Adelina Hasyim Adelina Hasyim Adi Novri Yanto AGUNG PRANOTO Agus Pahrudin Alben Ambarita Aldriawan, Mochamad Rizky Amilah, Susie Amirudin Prawita Ana Rosada Andiani Mutiara Dewi, Andiani Anggara Satria Putra, Anggara Satria Ani Marlena Ansori, Ahmad Faisol Anu, Maria Yuliani Aprilia Tri Aristina Apriliyani Apriliyani Ario Imandiri, Ario Asrin Asrin Ayu Reza Ningrum Azizah Apriyani Azkiya Maisari Bambang Supranoto Bambang Tri Purwanto Bobsaid, Jihan Bujang Rahman Christin Angelina Febriani Dadang Purwatmaja Darmiyati Darmiyati Darsono Darsono Darsono Darsono Defti Arlen Devi Ariani Devitri, Nabilah Apsari Dian Afuarita Dian Handayani ST Diyah, Nuzul Wahyuning Dodi Setiawan Dwi Istiyani Dwi Rohmanita Dwiana Arista Eddy Purnomo Edy Edy Edy Purnomo Eka Fitri Yanti Erine Nur Maulidya Erlina Rupaidah Esti Hariani Fadhillah, Nurul Fauziyah Fauziyah Febby Rullya Rasyid Febri Annuryanti Fernanda, MA Hanny Ferry Fitri Indriani Fridayanti S, Septia Indah Ganjar Winata Habsari, Dwiyana Hadi Poerwono Hamidah Nurjamilah Handayani, Rosita Handoko Handoko Hanum, Siti Halima Hasan Hariri Herawati Herawati Heri Supriadi Herida Herida Herpratiwi Herpratiw I Ketut Suada I Putu Eka Amerta Iceu Mayasari Ide Lia Marzuki Idha Kusumawati Iding Iding Ika Leli Erawati Inayatullah Wijayanti Indaryani Indaryani, Indaryani Irawan Suntoro Ismu Sukamto Ivana Artha Nitza Jaelani, Mochammad Iqbal Khatimah, Siti Husnul Lies Elina Lolita Sary M Lukman Efendi M. Thoha B.S Jaya M. Thoha B.S. Jaya M.Pd S.T. S.Pd. I Gde Wawan Sudatha . M.Thoha B.S. Jaya Margo Wibowo Maria Lucia Ardhani Dwi Lestari Maulidah, Maya Hikmatul Maya Susanti Megawati Megawati Melanny Ika Sulistyowaty mey zulfia herman Muhammad Afif Mulyani, Nina Munandar, Tristiana Erawati Nareswari, Andwynanda Bhadra Noni Heriawati Novianti Novianti Nunuk Dian Anggraeni Nuraini Nuraini Nurjannah Nurjannah Nurul Atieka Nurwahidin, Muhammad Pargito Pargito Prihati, Ririek Prihatiningtyas, Setyo Pujiati Pujiati Putri, Yushalluuna Bekti Pasuka Putut Ary Sadewo R Gunawan Sudarmanto R. Gunawan Sudarmanto Raden Gunawan Sudarmanto Rahmi Fitrina Renni Suryani Retno Widyowati REZA SYEHMA BAHTIAR Risma M Sinaga Risma Margaretha Sinaga Risma MSinaga Risma. M Sinaga, Risma. M Riswanti Rini Riswanti Rini risya novertie Rita Yusnely Aris Riyanto MT Rizkyah, Chaerini Rizyanti Rizyanti Rosyidah, Shofiatur Rovitasari, Riska Sa'adi, Ashon Safitri, Tutut Sandiya, Arroofita Ani Sarah Marcelly H Harahap Setyawati, Eni Shaffiqa, Nurul Siddiq Dharmadi Sistiwati Sistiwati Siti Khoiriyah SITI LATIFAH Sukardiman Sukarjati Sukarjati, Sukarjati Sumadi Sumadi sunardi sunardi Supomo Kandar Susila Darmawati Susilawati Susilawati Suyatno Suyatno Syamsi Syamsi Syehma B, Reza Toto Poernomo, Achmad Trans Kasiono Tri Rohayati Trisnaningsih Trisnaningsih Tutik Sri Wahyuni Ujang Efendi Ulfiah Husni Anjari Ulum, Mufidatul Umi Tarsih Vevi Liasari Wahidin Wahidin Wahiduddin Wahiduddin Wahyuning Yantini Walidain, Mardhatillah Birrul Waluyo Waluyo Widyowati, Rr. Retno Windianto, Farhan Rizqi Wiwied Ekasari Yakub, Nikolas Yanti, Dhiny Easter Yesni Yesni Yuli Astuti Yulistian Arismunandar Yusuf, Helmy Zain, Bagus Perdana Kusuma Zulfah, Yasmin Zulfiyah Emaz