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Oil Palm Seed Coating with Enriched Trichoderma asperellum (T13) to Suppress Infection of Ganoderma boninense Pat. Gani Jawak; Eny Widajati; Endah Retno Palupi; Nutrita Toruan Mathius
Jurnal Perbenihan Tanaman Hutan Vol 6, No 2 (2018): Jurnal Perbenihan Tanaman Hutan
Publisher : Forest Tree Seed Technology Research & Development Center (FTSTRDC)/ Balai Penelitian dan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.582 KB) | DOI: 10.20886/bptpth.2018.6.2.121-132

Abstract

The attack Ganoderma boninense can caused stem rot of oil palms that occur at all stage of plant growth. Trichoderma asperellum endophytic can suppressed the attack of Ganoderma in a nursery by utilizing seed coating technology. The aim of this study was to determine the best formula of seed coating materials which is compatible with T. asperellum (T13). The first experiment consisted of two phases, namely, the first phase was testing the effectiveness and compatibility of T. asperellum through a mixing technique of  T. asperellum suspension with coating material. The best three result on the first phase (25 percent arabic gum, 1 percent CMC, and 3percent arabic gum + 1 percent gypsum) were used in the second phase of the experiment, which was testing the effectiveness and compatibility of T. asperellum through soaking technique in T. asperellum suspension that continued by coating. The three best result types of formulas from the second phase (1 percent CMC, 1.5 percent CMC, and 4,5 percent arabic gum + 1,5 percent gypsum) were used for the second experiment, namely testing the resistance of oil palm seed on Ganoderma. The results show that 25 percent arabic gum, 1 percent carboxy methyl cellulose (CMC), 1 percent arabic gum + 1 percent gypsum have a potency as coating of materials. The best formula for seed coating is soaking with T. asperellum + coating 1 percent CMC, 1.5 percent  CMC and arabic gum 4.5 percent + gypsum 1.5 percent. In addition, soaking seeds with T. asperellum + coating 1.5 percent CMC can enhance the ability of seed to grow up to 16.67 percent compared to the control, but did not effective in suppressing G. boninense infection during pre-nursery stage.
Ultrafine Bubbles Technology for Breaking Dormancy of Sandalwood Seeds (Santalum album L. Juliana Maia; Abdul Qadir; Eny Widajati; Yohannes Aris Purwanto
Jurnal Perbenihan Tanaman Hutan Vol 9, No 1 (2021): Jurnal Perbenihan Tanaman Hutan
Publisher : Forest Tree Seed Technology Research & Development Center (FTSTRDC)/ Balai Penelitian dan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/bptpth.2021.9.1.26-41

Abstract

Sandalwood seed has two types of dormancy, namely physical dormancy and physiological dormancy which is a combination of the Two-part is called morphophysiological dormancy. There is for breaks dormancy in sandalwood for earlier embryo maturation and elongation also it has hard and impermeable skin. Its structure consists of layers of thick-walled palisade-like cells especially on the outermost surface and the inside has a waxy coating and curse material.  The objective of this study was to break of seed dormancy with technology Ultrafine Bubbles (UFB) on the morphophysiological dormancy on sandalwood seeds. The experiments used a randomized complete block designed (RCBD) with 3 replications. The data were analyzed using ANOVA and will be continued using the DMRT test at the 5% level. The research was conducted from February - March 0f 2020. The results showed that immersion using UFB water with oxygen 20 ppm or either UFB free oxygen for 24 and 48 hours combined with physical scarification and chemical scarification could accelerate germination in 13 days after germination (appeared radicle), percentage of growth speed (GS) is 4.67%, maximum growth (MG) in 21 days after sowing is 66.67% with normal sprouts 2-4 leaves have grown.
PENGARUH UMUR PANEN RIMPANG TERHADAP PERUBAHAN FISIOLOGI DAN VIABILITAS BENIH JAHE PUTIH BESAR SELAMA PENYIMPANAN DEVI RUSMIN; M.R. SUHARTANTO; SATRIYAS ILYAS; DYAH MANOHARA; ENY WIDAJATI
Jurnal Penelitian Tanaman Industri Vol 21, No 1 (2015): Maret 2015
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v21n1.2015.17-24

Abstract

ABSTRAKSalah satu faktor yang menentukan daya simpan benih jahe putihbesar (JPB) adalah mutu. Mutu benih sangat ditentukan oleh tingkatkemasakan rimpang. Penelitian bertujuan untuk mengetahui pengaruhumur panen terhadap perubahan fisiologi dan viabilitas benih selamapenyimpanan. Penelitian dilaksanakan di Rumah Kaca dan LaboratoriumTeknologi Benih, Balai Penelitian Tanaman Rempah dan Obat sertaLaboratorium Pascapanen IPB Bogor, mulai bulan Juli 2012 sampaidengan Februari 2013. Rancangan yang digunakan adalah acak lengkap(RAL) dengan lima ulangan. Perlakuan yang diuji adalah tiga tingkat umurpanen benih 7, 8, dan 9 bulan setelah tanam (BST). Pengamatan dilakukanterhadap perubahan fisiologis (penyusutan bobot, persentase rimpangbertunas, tunas, kadar air, dan laju respirasi), serta viabilitas rimpang benih(daya tumbuh, tinggi, dan bobot kering bibit). Hasil penelitianmenunjukkan rimpang benih umur 7 dan 8 BST mempunyai daya simpanterbaik karena menghasilkan masing-masing total angka penyusutan bobotlebih rendah (24,65 dan 25,25%) dan tunas lebih pendek (0,30 dan 1,08cm) dibandingkan dengan umur panen 9 BST (27,13% dan 1,62 cm),selama 4 bulan disimpan. Masa dormansi rimpang benih JPB mulai pecahsetelah mengalami periode simpan 2 bulan. Pertumbuhannya mulaiseragam setelah 3 bulan simpan. Umur panen jahe 7 dan 8 BSTmempunyai derajat dormansi yang lebih tinggi dibanding 9 BST. Rimpangbenih umur panen 7, 8, dan 9 BST mempunyai daya tumbuh tinggi (>95%)dan pertumbuhan bibit seragam setelah 3 bulan disimpan.Kata kunci: Zingiber officinale Rosc., penyimpanan, benih, perubahanfisiologis, viabilitasABSTRACTOne of the factors that determine the storability of seed rhizome ofwhite big ginger (WBG) is quality. The quality is determined by thematurity levels of seed rhizome. The aim of the experiment was to observethe effect of harvesting time on physiological changes and seed viability ofWBG seed rhizomes during the storage. The experiment was conducted atGreen House and Seed Technology Laboratory of Indonesian Spice andMedicinal Crops Research Institute, Bogor and Postharvest Laboratory,IPB, from July 2012 to February 2013. The experiment was arranged in acompletely randomized design with five replications. The treatmentstested were three levels of WBG seed rhizome harvesting time: 7, 8, and 9month after planting (MAP). Variables observed were physiologicalchanges of seed rhizomes during the storage (weight loss, sproutingpercentage, shoot height, respiration rate, and moisture content) andviability (growth ability, height, and dry weight of the seedling). Theresults showed that seed rhizomes at 7 and 8 had the best storability, sinceit was produced each low rate of weight loss (24.65 and 25.25%), andshoots shorter (0.3 and 1.08 cm) than 9 MAP (27.13% and 1.62 cm), for 4months in storage. Dormancy of WBG seed rhizomes has been brokenafter 2 months in storage. Harvesting at 7 and 8 showed a degree ofdormancy higher than the harvesting age 9 MAP. Harvesting time at 7, 8,and 9 MAP had high growth ability (> 95%) and uniform seedling growthafter 3 months in storage.Keywords: Zingiber officinale Rosc., storage, seed, physiologicalchanges, viability
Kuantitas dan Kualitas Kecambah Sengon pada Beberapa Tingkat Viabilitas Benih dan Inokulasi Rhizoctonia sp. Achmad Achmad; Sri Suharyati Vityaningsih; Eny Widajati
Jurnal Silvikultur Tropika Vol. 3 No. 1 (2012): Jurnal Silvikultur Tropika
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.3.1.%p

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Quantity and Quality of Sengon Sprouts at Some Levels of Seed Viability and Rhizoctonia sp. InoculationThe purpose of this research was to evaluate the quantity and quality of sengon sprouts with several levels of  seed viability and inoculation of Rhizoctonia sp. Materials used was three lots of sengon seed with different levels of viability obtained from Forest Management Unit Pare - Kediri and  Rhizoctonia sp. isolate cultured on PDA media. The treatments tested were 3 levels of seed viability  and 4 levels of Rhizoctonia sp. inoculum. The results showed that seeds with higher viability levels (seed germination = SG 87 and 83 %) produce more sprouts, indicated by the germination percentage which was significantly higher than that of  seeds with lower viability. Rhizoctonia sp. inoculation decreased the number of sprouts, indicated by the germination percentage which was significantly lower. The attacked of Rhizoctonia sp. on sengon sprouts was not associated with the level of seed viability, the lowest inoculum levels (3 corkborer 10-1 ml media) caused high percentage of seedlings death (57%) even  at the seed with high level of viability (SG 87%). The quality of the sprouts were not influenced by the level of seed viability, indicated by the length of roots and hyphocotil of normal sprouts which was not significantly different of sprouts from the seed with higher viability level (SG 87%, root length  2,71 cm, hypocotile length 3,00 cm) than that of the seed with lower viability level (SG 56%, root length  2,38 cm, hypocotile length 2,66 cm).
Sistem Pendeteksi Viabilitas Benih Kacang Tanah Berdasarkan Luas Area HSV Color Haura Fikriyah Hakimah; Trisno Yuwono Putro; Sabar Pramono; Eny Widajati
IJEIS (Indonesian Journal of Electronics and Instrumentation Systems) Vol 11, No 2 (2021): Oktober
Publisher : IndoCEISS in colaboration with Universitas Gadjah Mada, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijeis.68731

Abstract

Peanut seed tetrazolium test evaluation is usually by eye and a microscope. This method has a weaknesses in the accuracy of reading the color intensity, and  is more subjective. The seeds was observed one by one so that the observation is not effective. To make observations more accurate, efficient, and effective, digital image processing can be applied to the seed viability evaluation. The method can be used was the detection of the Hue, Saturation, and Value color area in reading the red color pattern resulting from tetrazolium test.The result is the system can detect a maximum of 25  seeds with an operational time of 22-25 seconds in one detection. Seed classification is the seeds are predicted to normal, abnormal, and dead. The process of classifying seeds is identified based on the red color pattern resulting from the detection of the area of 4 HSV color ranges, namely red (175,100,20:180,255,255), pink (160, 100,20 : 174,150,255), white 1 (175,0,0 : 180,100,255), and white 2 (0,0,0 : 100,255,255). The results show that the accuracy of the system in reading the total number of seeds is 100% with the detection error of  HSV color area is 1.54%.
PENINGKATAN PRODUKSI DAN MUTU RIMPANG BENIH JAHE PUTIH BESAR MELALUI APLIKASI ZAT PENGATUR TUMBUH The Production and Quality Improvement of Big White Ginger Seed Rhizomes by Plant Growth Regulator Aplication Devi Rusmin; Muhammad Rahmad Suhartanto; Satriyas Ilyas; Dyah - Manohara; Eny - Widajati
Perspektif Vol 19, No 1 (2020): Juni 2020
Publisher : Puslitbang Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/psp.v19n1.2020.29-40

Abstract

Permasalahan utama dalam pengembangan tanaman jahe putih besar (JPB) adalah   terbatasnya ketersediaan rimpang benih bermutu dalam jumlah yang mencukupi, pada waktu diperlukan oleh pengguna. Permasalahan tersebut antara lain disebabkan oleh produksi dan mutu rimpang benih yang masih rendah, serta bobot rimpang benih yang cepat menyusut dan mudah bertunassaat di penyimpanan. Penulisan ini bertujuan untuk menginformasikan kepada pengguna tentang karakter pola pertumbuhan, keseimbangan hormonal dan perubahan fisiologis yang menjadi faktor perhatian utama dalam peningkatan produksi dan mutu JPB melalui aplikasi zat pengatur tumbuh (ZPT).Peningkatan produksi dan mutu dapat dicapai dengan penggunaan rimpang benih  bermutu yang diperoleh  melalui: penentuan pola pertumbuhan, pengaturan keseimbangan hormon, baik secara alami (pengaturan iklim mikro), maupun dengan pemberianZPT selama proses produksi di lapangan dan di penyimpanan. Beberapa hasil penelitian membuktikan bahwa: (1) Pola pertumbuhan tajuk dan rimpang JPB selama pembentukan dan perkembangannya secara umum diklasifikasikan atas  tiga fase yaitu: fase lambat 1–4 bulan setelah tanam (BST), cepat (> 4–6 BST), dan pemasakan (> 6 BST).Rimpang benih JPB umur 7 BST sudah dapat digunakan sebagai bahan tanaman. (2) Perbedaan lokasi tanam dan umur panen mempengaruhi pola keseimbangan hormon endogen tanaman (rasio hormonABA/GA dan ABA/sitokinin (Zeatin) dan mutu rimpang benih JPB. Rasio ABA/sitokinin (zeatin) yang lebih tinggi pada rimpang benih umur 7 BST (5,0) dan 8 BST (4,7) dibanding rimpang benih umur 9 BST (4,2) untuk rimpang benih asal Nagrak, sehingga mampu memicu dan mempertahankan dormansi sehingga benih JPB lebih tahan disimpan.  (3) Periode dormansi benih rimpang JPB pecah setelah disimpan selama 2 bulan dan merupakan periode kritis atau periode yang tepat untuk aplikasi perlakuan penundaan pertunasan. (4) Aplikasi PBZ 400 ppm meningkatkan produksi JPB yang dinyatakan dalam bobot basah (22%) dan jumlah rimpang cabang (68%), dengan karakter rimpang: kecil, ruas pendek dan bernas, serta meningkatkan mutu dan daya simpan dibanding tanpa PBZ. (5) Aplikasi PBZ 1000 ppm, pada suhu ruang simpan 20 – 22 ºC, dapat menekan susut bobot sebesar 15% dibanding kontrol, setelah disimpan selama 4 bulan dan dapat menekan persentase rimpang bertunas sebesar 26% setelah 3 bulan disimpan.Permasalahan utama dalam pengembangan tanaman jahe putih besar (JPB) adalah   terbatasnya ketersediaan rimpang benih bermutu dalam jumlah yang mencukupi, pada waktu diperlukan oleh pengguna. Permasalahan tersebut antara lain disebabkan oleh produksi dan mutu rimpang benih yang masih rendah, serta bobot rimpang benih yang cepat menyusut dan mudah bertunassaat di penyimpanan. Penulisan ini bertujuan untuk menginformasikan kepada pengguna tentang karakter pola pertumbuhan, keseimbangan hormonal dan perubahan fisiologis yang menjadi faktor perhatian utama dalam peningkatan produksi dan mutu JPB melalui aplikasi zat pengatur tumbuh (ZPT).Peningkatan produksi dan mutu dapat dicapai dengan penggunaan rimpang benih  bermutu yang diperoleh  melalui: penentuan pola pertumbuhan, pengaturan keseimbangan hormon, baik secara alami (pengaturan iklim mikro), maupun dengan pemberianZPT selama proses produksi di lapangan dan di penyimpanan. Beberapa hasil penelitian membuktikan bahwa: (1) Pola pertumbuhan tajuk dan rimpang JPB selama pembentukan dan perkembangannya secara umum diklasifikasikan atas  tiga fase yaitu: fase lambat 1–4 bulan setelah tanam (BST), cepat (> 4–6 BST), dan pemasakan (> 6 BST).Rimpang benih JPB umur 7 BST sudah dapat digunakan sebagai bahan tanaman. (2) Perbedaan lokasi tanam dan umur panen mempengaruhi pola keseimbangan hormon endogen tanaman (rasio hormonABA/GA dan ABA/sitokinin (Zeatin) dan mutu rimpang benih JPB. Rasio ABA/sitokinin (zeatin) yang lebih tinggi pada rimpang benih umur 7 BST (5,0) dan 8 BST (4,7) dibanding rimpang benih umur 9 BST (4,2) untuk rimpang benih asal Nagrak, sehingga mampu memicu dan mempertahankan dormansi sehingga benih JPB lebih tahan disimpan.  (3) Periode dormansi benih rimpang JPB pecah setelah disimpan selama 2 bulan dan merupakan periode kritis atau periode yang tepat untuk aplikasi perlakuan penundaan pertunasan. (4) Aplikasi PBZ 400 ppm meningkatkan produksi JPB yang dinyatakan dalam bobot basah (22%) dan jumlah rimpang cabang (68%), dengan karakter rimpang: kecil, ruas pendek dan bernas, serta meningkatkan mutu dan daya simpan dibanding tanpa PBZ. (5) Aplikasi PBZ 1000 ppm, pada suhu ruang simpan 20 – 22 ºC, dapat menekan susut bobot sebesar 15% dibanding kontrol, setelah disimpan selama 4 bulan dan dapat menekan persentase rimpang bertunas sebesar 26% setelah 3 bulan disimpan. ABSTRACT The main problems in the development of big white ginger plant (BWG) is the limited availability of quality seed rhizomes in sufficient quantities, at the time required by the user. Its caused by the production and quality of seed rhizomes are still low, and the seed rhizomes weight are rapidly shrinking and sprouting when in the storage. This Overview aims to inform users about the character of the pattern of growth, the balance of hormonal and physiological changes that are primarily focused on the production and seed quality improvement BWG through the application of plant growth regulator (PGR). Increased production and quality can be achieved by the use of quality seed rhizomes obtained through: determination of growth patterns, hormonal balance regulation, both naturally (microclimate regulation), as well as by application of growth regulators (ZPT) during the production process in the field and in storage. Some research results showed that: (1) The growth pattern of the canopy and GWB seed rhizomes during its formation and development is generally classified into three phases: slow phase 1-4 months after planting (MAP), fast (> 4-6 MAP), and maturty (> 6 BST). (2) Differences in planting location and harvest age affect the balance pattern of plant endogenous hormones (ABA / GA and ABA / cytokinin (zeatin) hormone ratios) and the BWG seed rhizomes quality. ABA / cytokinin ratios are higher in BWG seedlings aged 7 MAP (5.0) and 8 MAP (4.7) compared to 9 MAP (4.2) for seed rhizomes from Nagrak, so they are able to trigger and maintain dormancy so Its are more resistant to storage. (3) The dormancy period of BWG seed rhizomes break after stored for 2 months and this is a critical period or an appropriate period for sprouting inhibition treatment. (4) Application of PBZ 400 ppm increased production and quality of BWG seed rhizomes, namely: wet weight (22%) and number of branch rhizomes (68%) with rhizome characteristics: small, short and filled out internodes compared without PBZ. (5) Application of PBZ 1000 ppm, at a storage temperature of 20-22 ºC, can reduce weight loss by 15% compared to control, after stored for 4 months and also can reduce the sprouting percentage of rhizomes by 26% after stored for 3 months.
Peningkatan vigor kelapa sawit melalui pengayaan kecambah dengan Trichoderma asperellum, Cendawan Mikoriza Arbuskular dan Enterobacter sacchari Increasing of oil palm seedling vigor through seed enrichment with Trichoderma asperellum, Arbuscular Mycorrhizal Fungi, and Enterobacter sacchari) Esty Puri UTAMI; Eny WIDAJATI; Endah Retno PALUPI; Nurita TORUAN-MATHIUS
E-Journal Menara Perkebunan Vol 86, No 2 (2018): Oktober 2018
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1019.312 KB) | DOI: 10.22302/iribb.jur.mp.v86i2.267

Abstract

Oil palm is a leading commodity of the plantation sector in Indonesia. Improving the quality of oil palm still be carried out to increase production. Seed technology can be used as an effort to improve the quality of oil palm seeds. The aim of this experiment was to determine the effect of seed enrichment with consortium of three microbes to increase vigor of oil palm seedling in pre nursery stage. The experiment design of this reseach was using completely randomize block design consisted of two factors. The first was seed coating consist of two factors, ie: coated seed and uncoated seed. Second was seed enrichment consist of eight factors, ie: control, enrichment with E. sacchari, abruscular mycorrhizal fungi (AMF), T. asperellum, E. sacchari+ AMF, E. sacchari+ T. asperellum, AMF + T. asperellum, E. sacchari+ AMF + T. asperellum. The result showed that enrichment with consortium of three microbes could increase vigor of oil palm seedling based on seedling germination, rate of germination, palm height, and numbers of survival seedling.[Keywords:biological agent, compatibility, diazotroph] Abstrak *) Penulis korespondensi: eny.widajati61@gmail.com Kelapa sawit adalah komoditas unggulan sektor perkebunan di Indonesia. Peningkatan mutu kelapa sawit terus dilakukan agar mening-katkan produksinya. Teknologi benih dapat digu-nakan sebagai salah satu upaya peningkatan mutu benih kelapa sawit. Penelitian ini bertujuan untuk menetapkan pengaruh pengayaan konsorsium tiga mikroba, E. sacchari,T. asperellumdan cendawan mikoriza arbuskular (CMA) dan pelapisan kecambah terhadap peningkatan vigor bibit kelapa sawit di pre nursery. Percobaan dirancang dengan rancangan acak kelompok dengan dua faktor, yaitu pelapisan dan pengayaan. Pelapisan terdiri dari dua taraf, yaitu dengan pelapisan dan tanpa pelapisan. Pengayaan terdiri dari 8 taraf, yaitu kontrol, pengayaan dengan E. sacchari, CMA, T. asperellum, E. sacchari+ CMA, E. sacchari + T. asperellum,CMA + T.asperellum,E. sacchari+ CMA + T. asperellum. Hasil penelitian menunjukkan bahwa pengayaan dengan konsor-sium tiga mikroba dapat meningkatkan vigor bibit kelapa sawit berdasarkan parameter daya tumbuh, kecepatan tumbuh, tinggi bibit, dan jumlah bibit yang hidup.  [Kata kunci:    agen hayati,    diazotrop, kompa-tibilitas]
Aplikasi Teknologi Citra untuk Memilah Tingkat Kerusakan Kulit Benih Kedelai Varietas Anjasmoro Muhamad Rezki Mauladi; Trisno Yuwono Putro; Peni Handayani; Eny Widajati
Prosiding Industrial Research Workshop and National Seminar Vol 11 No 1 (2020): Prosiding 11th Industrial Research Workshop and National Seminar (IRWNS)
Publisher : Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (570.312 KB) | DOI: 10.35313/irwns.v11i1.1969

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Kerusakan benih kedelai akibat proses pengolahan benih terjadi saat perontokan, pembersihan dan sortasi benih dengan mesin berpengaruh terhadap mutu fisik benih terutama kerusakan pada kulit benihnya. Salah satu cara untuk evaluasi tingkat kerusakan kulit benih kedelai dengan pewarnaan Fast Green yang dapat memperlihatkan warna hijau sebagai acuan untuk penentuan tingkat kerusakan. Selanjutnya, untuk memudahkan proses pengelompokan benih digunakan pengelolaan citra dengan image segmentation dengan basis RGB dengan cara memisahkan kelompok warna yang diinginkan berdasarkan jangkauan warna yang ditentukan berupa empat kelompok warna. Selanjutnya akan dihitung area warna sebagai pertimbangan dalam penentuan warna yang dominan. Benih kedelai yang digunakan adalah varietas Anjasmoro. Terdapat empat kriteria tingkat kerusakan yaitu tidak ada kerusakan (coklat muda asli warna kulit) RGB (112,94,54 - 134,111,70), rusak berat (warna hijau pekat) RGB (13,51,36 - 31,53,41), rusak sedang (warna hijau) RGB (34,72,47 - 31,53,41), dan rusak ringan (hijau muda) RGB (66,75,46 - 95,100,60).
Pemanfaatan Bakteri Probiotik untuk Menekan Infeksi Colletotrichum acutatum dan Meningkatkan Mutu Benih Cabai (Capsicum annuum L.) Selama Penyimpanan Anna Tefa; Eny Widajati; Muhamad Syukur; Giyanto Giyanto
Savana Cendana Vol 1 No 01 (2016): Savana Cendana (SC) - January 2016
Publisher : Fakultas Pertanian, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (529.589 KB) | DOI: 10.32938/sc.v1i01.10

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This study aimed to obtain probiotic bacteria that are antagonistic bacteria to Colletotrichum acutatum and to improve chilli seeds quality. The purpose of this study was to obtain probiotic bacteria antagonistic to C. acutatum and prevent seed-borne diseases. The layout of the experiment arranged in completely randomized design factorial design with two factors. The first factor is the seed coating that consists of 6 levels i.e control / without coating (c0), coating without bacteria (c1), coating Bacillus sp. (c2), coating Pseudomonas sp. (c3), coating Actinomycetes sp. (c4) and fungicidal coating (c5). The second factor is the storage period is 0, 1, 2, 3, 4 and 5 months. The results showed that three bacteria that are antagonistic to C. acutatum, i.e.Actinomycetes sp. (ATS6) having inhibition percentage of56, 8%, Bacillus sp. (B48) having inhibition percentage 56,7% and Pseudomonas sp. (P12) having inhibition percentage 46,7%. Application of probiotic bacteria increased viability and the health of chilli seed for storage period of 5 months, seen from maximum growth potential 80-84%, germination capacity 76-78,7%, infected seeds in coating of Actinomycetes sp., 2,6% and Bacillus sp., 6,7%.Bacillus sp. contained 91,8 ppm indole acetic acid (IAA) and 103,2 ppm giberelins (GA3), Actinomycetes sp. contained 89,6 ppm IAA and 92,5 ppm giberelin and Pseudomonas sp. contained 68,9 ppm IAA and 69,2 ppm giberelin.
Pendugaan Kemunduran Benih dengan Uji Fisiologi dan Biokimiawi Gani Jawak; Eny Widajati; Devi Liana; Tri Astuti
Savana Cendana Vol 7 No 04 (2022): Savana Cendana (SC) - October 2022
Publisher : Fakultas Pertanian, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.083 KB) | DOI: 10.32938/sc.v7i04.1921

Abstract

The seed viability test is one of the routine tests carried out in the seed certification process. Decreasing the viability of seeds can be done biochemically and physiologically. This research aimed to study of seeds deterioration through physiological and biochemical tests. This study used two lots of long bean seeds (high and low lot). Physiologically, the seed viability test was carried out by means of germination test and biochemical by means of respiration test using tetrazolium (TTZ) staining technique and titration method. The experiment designed by completely randomized design one factor. Data were analyzed by anova test and Pearson correlation. The results of research showed that the seed lot treatment was significantly on the tetrazolium test and the total dry weight of sprouts, while the vigor index, seed germination, and respiration index measurements did not show significant. There was a high positive correlation in the high respiration rate of high seed lots and low seed lot seeds on total dry weight of germination, germination, vigor index, and tetrazolium test. Therefore, biochemical tests with TTZ and respiration with titration methods can be used as alternatives to predict rapid deterioration of seeds.
Co-Authors , Dina , Ramlafatma ,, Saipulloh . Giyanto Abdjad Asih Nawangsih Abdul Qadir Achmad Achmad Anak Agung Keswari Krisnandika Anggraini, Dwi Vista Annisa Nursoleha Ansella, Yohanda Anugrah, Mertya Arif, Mohamad Awang Maharijaya Bambang Sapta Purwoko Baran Wirawan Blair Moses Kamanga Cici Tresniawati Dahlan, Ade Muhamad Daniel Happy Putra Danu Kuncoro Devi Liana DEVI RUSMIN DEVI RUSMIN Devi Rusmin Didy Sopandie Dirgahani Putri Dwi Gery Febriyan Dwi Guntoro Dyah - Manohara DYAH MANOHARA Dyah Manohara Edi Santosa Edi Santosa Edi Santosa Edy Suprianto Efendi, Darda Endah Retno Palupi Endah Retno Palupi ENDANG MURNIATI Esti Rahayu Esty Puri UTAMI Esty Putri Utami Esty Putri Utami Faiza C. Suwarno Faiza C. Suwarno Faiza Chairani Suwarno Febriani, Lilis Yati Febriyan, Dwi Gery Gani Jawak Giyanto Giyanto Giyanto Giyanto Giyanto, dan Gundala, Belangie Tuahte Hadi, Putra K Haura Fikriyah Hakimah Herliyana Indahwardani Imroatus Sa’adah Indahwardani, Herliyana Iswari Saraswati Dewi Juliana Maia Kamanga, Blair Moses Kikin H Mutaqin Kresnamurti Kurniasih Krisnandika, A. A. Keswari Kusumastuti, Hamiddah Intan Kusumastuti, Shinta Nugraheni Lilis Yati Febriani M.R. SUHARTANTO M.R. SUHARTANTO, M.R. Maisaroh Tanjung MANOHARA, DYAH Manohara, Dyah - Maryati Sari Masitadewi, Ervin Mathius, dan Nurita Toruan Meity Suradji Sinaga Memen Surahman Mia Kosmiatin Mohamad Arif Mohamad Arif Mohamad Rahmad Suhartanto Mubarak, Farahdina Muhamad Rezki Mauladi Muhamad Syukur Muhammad Afif Muhammad Machmud Nadiya Iftiwata Rahmah Nelly Fridayanti Ni Made Armini Wiendi Nizaruddin . Norul Dewi Susanti Nur Syam Ismaniza A., Siti Nur Syam Ismaniza A. Nurita TORUAN-MATHIUS Nutrita Toruan Mathius Okti Syah Isyani Permatasari Peni Handayani Permatasari, Okti Syah Isyani Pitri Ratna Asih Pradana, Adhitya Vishnu Pramudita, Listya Purwoko, Bambang Sapta Purwoko Putra K Hadi Putri Mian Hairani Putro, Trisno Yuwono Qadir , Abdul Qadir, Abdul Qudus Sabha Adhinugraha R Pelealu, Rian Virvian Hidayat R. Palupi, Endah Rafi Fauzan Raga, Yuan Rahmah, Nadiya Iftiwa Riski Meliya Ningsih RUSMIN, DEVI RUSMIN, DEVI Sabar Pramono Sani, Mutiyara Sarah Desmia Muchtar Sarah Desmia Muchtar Satrias Ilyas SATRIYAS ILYAS Satriyas Ilyas Selly Salma Selly Salma Shinta Nugraheni Kusumastuti Siti Fadhilah, Siti Sobir Sobir Sri Suharyati Vityaningsih Sri Wilarso Budi SUHARTANTO, M.R. Suhartanto, Muhammad Rahmad Sukartini Supijatno Surya Kurnia Putra, Dicky Suwarno, Faiza C. Tanjung, Maisaroh Tantri Palupi Tati Budiarti Tefa, Anna Tefa, Anna Tri Astuti Trikoesoemaningtyas Trisno Yuwono Putro Wawan Hermawan Willy Bayuardi Suwarno Yuyuk Agung Lastiandika Yuyuk Agung Lastiandika