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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Jurnal Silvikultur Tropika (JST) Indonesian Journal of Electronics and Instrumentation Systems JURNAL PERBENIHAN TANAMAN HUTAN Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat Perspektif : Review Penelitian Tanaman Industri AGRIVITA, Journal of Agricultural Science Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat SC (Savana Cendana) : Jurnal Pertanian Konservasi Lahan Kering Jurnal Geografi, Edukasi dan Lingkungan Jurnal Agrotek Tropika Journal of Tropical Crop Science Menara Perkebunan Jurnal Agrotek Lestari Jurnal Penelitian Tanaman Industri (Littri) Perspektif, Review Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat Industrial Research Workshop and National Seminar
Eny Widajati
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti, Kampus IPB Darmaga, Bogor 16680, Telepon & Faksimili +62-251-8629353
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Daya Simpan Benih Botani Bawang Merah (Allium cepa var. agregatum) Setelah Invigorasi Qudus Sabha Adhinugraha; Eny Widajati; Endah Retno Palupi
Jurnal Agrotek Tropika Vol 12, No 4 (2024): JURNAL AGROTEK TROPIKA VOL 12, NOVEMBER 2024
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v12i4.7715

Abstract

Benih botani bawang merah (TSS) merupakan alternatif bahan tanam dalam budidaya bawang merah.  Penggunaan TSS sebagai bahan tanam memiliki keunggulaan dibandingkan dengan benih asal umbi, antara lain kebutuhan benih yang lebih sedikit, dapat memutus siklus patogen, tidak ada masa dormansi, dan dapat disimpan dalam jangka waktu lebih dari satu tahun. Walaupun dapat disimpan dalam jangka waktu yang lama, namun TSS tetap akan mengalami deteriorasi atau kemunduran yang dapat menyebabkan penuruan mutu benih.   Salah satu upaya yang dapat dilakukan untuk meningkatkan kembali mutu benih adalah dengan perlakuan invigorasi KNO3.  Saat ini peran invigorasi terhadap daya simpan benih TSS masih belum diketahui.  Penelitian ini dilaksanakan Laboratorium Penyimpanan dan Pengujian Mutu Benih IPB University, Indonesia. Penelitian ini bertujuan untuk mengetahui informasi daya simpan TSS setelah invigorasi dan pengeringan.  Percobaan disusun menggunakan rancangan tersarang (nested design) dua faktor.  Faktor pertama adalah kondisi ruang simpan benih yang terdiri atas tiga taraf, yaitu suhu 28±2˚C, RH 73±7% (kondisi kamar); suhu 18±2 ˚C, RH 61±7% (kondisi ruang ber-AC); dan suhu 8±2˚C, RH 24±8% (kondisi lemari es). Faktor kedua adalah jenis kemasan, yang terdiri atas dua jenis, yaitu kemasan alumunium foil (ketebalan 0,08 mm) dan kemasan plastik polipropilen (PP) (ketebalan 0,06 mm). Faktor jenis kemasan tersarang pada faktor kondisi ruang simpan benih.    Hasil penelitian menunjukkan bahwa Mutu TSS pasca invigorasi dapat dipertahankan selama 16 MSS dalam ruang ber-AC (suhu 18±2 ˚C, RH 61±7%) dengan kemasan alumunium foil atau lemari es (suhu 8±2˚C, RH 24±8%) dengan kemasan alumunium foil atau plastik PP. 
Protocol Development for Assessing Seed Moisture Content and Germination Testing in Amorphophallus muelleri Blume Fadhilah, Siti; Widajati, Eny; Ilyas, Satriyas; Palupi, Endah Retno; Qadir, Abdul
Journal of Tropical Crop Science Vol. 12 No. 01 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.01.132-144

Abstract

Seed quality testing involves the evaluation of germination capacity under a standardized moisture content. However, limited reports have been found for seed testing methodology, especially for Amorphophallus muelleri. Standardizing the seed testing method for A. muelleri is essential to ensure repeatability, reproducibility, and reliable seed germination results. This study aimed to develop procedures for assessing seed moisture content and germination tests. Three seed lots were used for seed moisture content determination. The necessity for seed cutting and efficacy of high-temperature oven methods (133°C for 4, 5, and 6 h) were compared to low-temperature oven methods (103°C for 17 h). Five lots of seeds of different ages were used for the seed germination test using the between-paper method in a constant temperature germinator of 25 and 30 °C. There was no significant difference in the moisture content between the uncut/whole and the seeds cut. The high temperature of 133°C for four hours is an alternative to the low temperature. Germination rates at 25 and 30°C had no significant difference, i.e., 84 to 97% and 83 to 99%, respectively. However, at 30°C, it was shorter to 50% germination (T50) and germination period. Preheating the seeds for four weeks using the dry heat method shortened the seed germination time from 57 to 37 days. Seed lot exposed to dry heat had half the time required to reach T50, or 16.4 to 31.9 days after treatment, with a germination period of 37 to 52 days. The first count of germination at 25°C without preheating was 47 days after planting, and the final count was 73 days after planting, whereas at 30OC, it was 37 and 57 days after planting. The first and final count for germination with preheating treatment at both temperatures was 20 DAP and 37 DAP.
Cytological Deterioration of Bitter Gourd (Momordica charantia L.) Pollen During Storage and Its Impact on Effectiveness for Seed Production Kusumastuti, Hamiddah Intan; Widajati, Eny; Palupi, Endah Retno
Journal of Tropical Crop Science Vol. 12 No. 02 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.02.303-313

Abstract

Pollen storage plays an important role in the hybrid seed production of bitter gourd, but a primary challenge is the rapid decline in pollen viability. This research aimed to investigate the mechanisms of bitter gourd pollen deterioration during storage and to assess the effectiveness of stored pollen for hybrid seed production. The study was conducted at the Leuwikopo Research Station, IPB University, from September 2018 to June 2021. Bitter gourd pollen was collected at anthesis and stored in a deep freezer at -21±3ºC. Pollen viability was observed at 0, 2, 4, 6, 8, 10, and 12 weeks after storage (WAS), while pollen ultrastructure was examined at 0, 4, 8, and 12 WAS using a transmission electron microscope. The stored pollen was then used for pollination, observations were made on fruit set, seed set, and seed quality. The viability of bitter gourd pollen declined rapidly following dehydration before storage. The declining pollen viability during storage was due to the degeneration of the intine, followed by mitochondrial deterioration. The cristae became disorganised, leading to the dissolution of the cristae and outer membrane of mitochondria. Storing the pollen in a deep freezer (-21±3ºC) after dehydration did not prevent a further decline in pollen viability. Bitter gourd pollen stored for more than 2 weeks at -21±3ºC is ineffective for seed production. Cucurbit pollen remains viable for only two weeks; thus, fresh pollen is more practical for hybrid seed production. Development of more effective pollen storage methods is necessary to prolong pollen viability and enhance breeding efficiency. 
Development of Rapid Viability Test Using Urine Sugar Analysis Paper for Peanut (Arachis hypogaea L.) Seeds Mubarak, Farahdina; Suhartanto, Mohamad Rahmad; Widajati, Eny
Journal of Tropical Crop Science Vol. 12 No. 02 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.02.378-387

Abstract

Seed testing is important for determining seed quality. Seed testing must be easy, quick, and accurate. This study aimed to develop a rapid method for assessing peanut seed quality using Urine Sugar Analysis Paper (USAP). USAP is commonly used for detecting human urinary glucose and is currently being developed for evaluating seed quality. Three experiments were conducted. Experiment 1 investigated the most effective seed-cutting size (whole, halved, and seeds cut into six pieces) and soaking periods (0, 3, 6, and 9 hours) based on glucose and protein leakage, as well as specific gravity of the soaking water. The optimal method identified in Experiment 1 was then used to test all five seed lots in USAP Experiment 2, which aimed to evaluate the effectiveness of seed quality testing using the USAP method at various levels of seed viability. Experiment 3 investigated the correlation between USAP test results and other viability and vigor test parameters, including germination percentage, first count germination, speed of germination, and electrical conductivity. The results of experiments indicated that cutting seeds into six pieces and 6 6-hour soaking period was the most optimal. Experiment 3 showed that USAP testing correlated with viability and vigor testing across various peanut varieties. Protein and density testing using USAP were negatively correlated with germination percentage, first count germination, and germination speed parameters, but positively correlated with electrical conductivity. However, glucose leakage was not detectable using USAP.
Peningkatan vigor kelapa sawit melalui pengayaan kecambah dengan Trichoderma asperellum, Cendawan Mikoriza Arbuskular dan Enterobacter sacchari Increasing of oil palm seedling vigor through seed enrichment with Trichoderma asperellum, Arbuscular Mycorrhizal Fungi, and Enterobacter sacchari) Esty Puri UTAMI; Eny WIDAJATI; Endah Retno PALUPI; Nurita TORUAN-MATHIUS
Menara Perkebunan Vol. 86 No. 2 (2018): 86 (2), 2018
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v86i2.267

Abstract

Oil palm is a leading commodity of the plantation sector in Indonesia. Improving the quality of oil palm still be carried out to increase production. Seed technology can be used as an effort to improve the quality of oil palm seeds. The aim of this experiment was to determine the effect of seed enrichment with consortium of three microbes to increase vigor of oil palm seedling in pre nursery stage. The experiment design of this reseach was using completely randomize block design consisted of two factors. The first was seed coating consist of two factors, ie: coated seed and uncoated seed. Second was seed enrichment consist of eight factors, ie: control, enrichment with E. sacchari, abruscular mycorrhizal fungi (AMF), T. asperellum, E. sacchari+ AMF, E. sacchari+ T. asperellum, AMF + T. asperellum, E. sacchari+ AMF + T. asperellum. The result showed that enrichment with consortium of three microbes could increase vigor of oil palm seedling based on seedling germination, rate of germination, palm height, and numbers of survival seedling.[Keywords:biological agent, compatibility, diazotroph] Abstrak *) Penulis korespondensi: eny.widajati61@gmail.com Kelapa sawit adalah komoditas unggulan sektor perkebunan di Indonesia. Peningkatan mutu kelapa sawit terus dilakukan agar mening-katkan produksinya. Teknologi benih dapat digu-nakan sebagai salah satu upaya peningkatan mutu benih kelapa sawit. Penelitian ini bertujuan untuk menetapkan pengaruh pengayaan konsorsium tiga mikroba, E. sacchari,T. asperellumdan cendawan mikoriza arbuskular (CMA) dan pelapisan kecambah terhadap peningkatan vigor bibit kelapa sawit di pre nursery. Percobaan dirancang dengan rancangan acak kelompok dengan dua faktor, yaitu pelapisan dan pengayaan. Pelapisan terdiri dari dua taraf, yaitu dengan pelapisan dan tanpa pelapisan. Pengayaan terdiri dari 8 taraf, yaitu kontrol, pengayaan dengan E. sacchari, CMA, T. asperellum, E. sacchari+ CMA, E. sacchari + T. asperellum,CMA + T.asperellum,E. sacchari+ CMA + T. asperellum. Hasil penelitian menunjukkan bahwa pengayaan dengan konsor-sium tiga mikroba dapat meningkatkan vigor bibit kelapa sawit berdasarkan parameter daya tumbuh, kecepatan tumbuh, tinggi bibit, dan jumlah bibit yang hidup.  [Kata kunci:    agen hayati,    diazotrop, kompa-tibilitas]
Konsentrasi dan Waktu Aplikasi Paklobutrazol, Pengaruhnya terhadap Pertumbuhan dan Mutu Benih Sorgum (Sorghum bicolor (L.) Monch) Anggraini, Dwi Vista; Widajati, Eny; Sari, Maryati; Supijatno
Buletin Agrohorti Vol. 13 No. 3 (2025): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrob.133.65443

Abstract

Sorgum varietas Samurai memiliki tinggi tanaman mencapai 198.7 cm, sehingga mudah rebah serta menyulitkan pemeliharaan dan pemanenan. Aplikasi paklobutrazol bertujuan menghambat pertumbuhan tinggi tanaman serta meningkatkan kualitas fisiologis benih. Penelitian ini bertujuan menentukan konsentrasi dan waktu aplikasi paklobutrazol yang tepat untuk menghasilkan benih sorgum bermutu tinggi. Penelitian dilaksanakan dari April 2023 hingga Februari 2024 di kebun percobaan Lewikopo. Konsentrasi paklobutrazol yang diberikan adalah 1000 ppm dan 1500 ppm, waktu aplikasi pada umur 5 dan 7 minggu setelah tanam.  Mutu benih diuji pada saat panen dan setelah penyimpanan (1, 2, dan 3 bulan). Aplikasi paklobutrazol 1000 ppm pada umur 5 minggu setelah tanam merupakan perlakuan yang tepat untuk menekan pertumbuhan tinggi tanaman. Perlakuan dosis paklobutrazol ini menghasilkan peningkatan yang signifikan pada diameter batang, panjang daun, dan lebar daun serta mutu benih yaitu daya berkecambah dan bobot kering kecambah normal. Aplikasi paklobutrazol secara nyata menurunkan kandungan giberelin dalam benih. Aplikasi paklobutrazol 1500 ppm pada 7 minggu setelah tanam menurunkan mutu benih setelah simpan berdasarkan tolok ukur daya berkecambah, kecepatan tumbuh, dan potensi tumbuh maksimum. Kata kunci: daya berkecambah, giberelin, kecepatan tumbuh
Seeds morphology, anatomy, and quality of Musa balbisiana and Musa acuminata after storage Anugrah, Mertya; Qadir, Abdul; Widajati, Eny; Sukartini
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 53 No. 2 (2025): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v53i2.59954

Abstract

The fluctuations in seed food reserves can trigger a cascade of physiological and biochemical events that ultimately manifest as discernible changes in seed morphology and anatomy.  The purpose of this research was to evaluate seeds morphology, anatomy, and quality of Musa balbisiana “Klutuk NTT” and Musa acuminata “Sumatrana” accessions and their storability. Morphological evaluation included seed size and the weight of 100 grains. Anatomical evaluations were carried out for seed structure. The seed quality of the Klutuk NTT banana accessions was observed after being stored for 5, 25, and 41 months, while the Sumatrana banana accessions were observed after being stored for 5, 23, and 41 months. The Klutuk NTT seeds are sized 4 mm to 5.8 mm, while Sumatrana seeds are sized 4 mm to 5 mm. The Klutuk NTT and Sumatrana seeds had a seed coat, endosperm, embryo, micropyle, inner and outer integuments, chalazal, and operculum. The embryonic structure of both Klutuk NTT and Sumatrana seeds exhibited an inverted T-shape. After storage, the two accessions showed significant differences in moisture content, germination rate, vigor index, and seedling growth performance. The Klutuk NTT accessions demonstrated a significant increase in moisture content, germination rate, vigor index, and seedling emergence uniformity, suggesting improved seed performance after storage. In contrast, the Sumatrana accessions showed a significant decline in all measured parameters, except for moisture content. These results suggest that the Klutuk NTT accession may possess a physiological seed dormancy mechanism. Keywords: Banana; germination; true seed; viability; vigor
Development of Tetrazolium Test as Vigor Analysis Method of True Seed of Shallot Afifah, Nurul; Widajati, Eny; Palupi, Endah Retno
Jurnal Hortikultura Indonesia (JHI) Vol. 11 No. 2 (2020): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.11.2.120-130

Abstract

Uji tetrazolium (TZ) merupakan salah satu metode untuk mengestimasi viabilitas dan vigor benih. Penelitian ini bertujuan untuk memperbaiki metode analisis vigor pada benih botani bawang merah. Pada penelitian ini digunakan dua varietas benih bawang merah, yaitu Trisula dan Tuk-tuk. Pre-treatment dalam uji tetrazolium benih bawang merah dilakukan dengan perendaman benih dalam air selama 18 jam. Sebelum pewarnaan benih disiapkan dengan menggores sisi lurus secara longitudinal sebagai satu-satunya irisan tipis, dan sisi cembung benih ditusuk dengan scalpel. Benih diwarnai selama 18 jam dalam larutan tetrazolium pada suhu 1% suhu 30 oC dalam kondisi gelap. Pola pewarnaan benih diklasifikasikan dalam empat kategori yaitu empat pola normal kuat sebagai kriteria benih vigor, lima pola normal lemah, tujuh pola abnormal, dan empat pola benih mati. Parameter fisiologis viabilitas dan vigor berkorelasi erat dengan hasil pola vigor/ normal kuat. Pola normal kuat memiliki koefisien korelasi (r) yang tinggi dengan parameter uji fisiologis di laboratorium termasuk indeks vigor (r= 0.882), bobot kering kecambah normal (r= 0.899), dan kecepatan tumbuh (r= 0.875). Hal ini mengindikasikan pola pewarnaan vigor dapat memprediksi vigor benih.
Invigoration Increased Quality and Storability of True Seed of Shallot (Allium ascalonicum L.) Adhinugraha, Qudus Sabha; Widajati, Eny; Palupi, Endah Retno
Journal of Tropical Crop Science Vol. 9 No. 02 (2022): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.9.02.145-155

Abstract

Seed invigoration is a pre-planting seed treatment by balancing the seed water potential and stimulating their metabolic activities so that the seeds germinate simultaneously. In this study, invigoration treatments were applied to improve the quality of the true seeds of shallots (TSS) that had started to deteriorate. Invigoration increases the moisture content of the seeds so the seeds need to be re-dried to extend their storability. The aims of the study were to determine the best invigoration treatment and the drying method to maintain the benefit of invigoration, and to prolong TSS storability after invigoration and drying. This research was conducted at the Laboratory of Seed Quality Testing, IPB University, Indonesia, and consisted of three experiments, i.e., invigoration of deteriorating TSS to improve their viability and vigor, drying after invigoration to extend seed storability, and seed storability after invigoration and drying treatments. The first experiment was arranged in completely randomized design with one-factor (invigoration solutions), i.e. control, medium containing 3% KNO3, 50 ppm GA3, 0.5 % ZnSO4 or PEG6000 -10 bar. The second experiment was conducted using a two-factor completely randomized design, drying temperature (20˚C, 30˚C, and 40˚C), and drying duration (8 hours, 16 hours, 32 hours, and 48 hours). The third experiment was arranged in a nested design with two factors. The first factor was a seed storage condition, i.e., ambient temperature, air-conditioned room, and refrigerator. The second factor was seed packages, i.e., aluminum foil and polypropylene plastic. Non-invigorated seeds were used as control. The studies were conducted on two shallot varieties separately, “Trisula” and “Lokananta”, except in the third experiment which only used “Lokananta”. The results showed that invigoration in 3 % KNO3 and 50 ppm GA3 effectively improved the vigor of deteriorating TSS. Drying the seeds at 40˚C for 8 hours was the most effective method to reduce seed moisture content without reducing the benefit of invigoration. Storing the invigorated TSS in an air-conditioned room with aluminum foil packaging, or in a refrigerator with aluminum foil or plastic packaging until 14 weeks maintained the benefit of invigoration. The quality of the invigorated TSS was maintained until 14 weeks in an air-conditioned room with aluminum foil packaging, or in a refrigerator with aluminum
Mechanism and Persistence Dormancy of Ground Cherry Seeds (Physalis peruviana L.) at Different Maturity Stages Pradana, Adhitya Vishnu; R. Palupi, Endah; Qadir, Abdul; Widajati, Eny
Journal of Tropical Crop Science Vol. 9 No. 03 (2022): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.9.03.183-192

Abstract

Ground cherry (Physalis peruviana) seeds have a period of dormancy after ripening. A study was conducted to understand the mechanism of dormancy, the duration of after ripening persistence, and to determine the effective methods to break dormancy. The study was conducted in an experimental field at Ciwidey, West Java, Indonesia, and the Seed Testing Laboratory, Faculty of Agriculture, Department of Agronomy and Horticulture, IPB University, in February to November 2020. The first study examined the effects of the storage temperatures, i.e. 20 ±2 oC and 25 ± 2oC, and the seed maturity stages, i.e. 49 days after anthesis (DAA), 58 DAA and 62 DAA. Several methods to break seed dormancy were tested, i.e. soaking seeds for 24 hours in distilled water, in 0.5% or 1% KNO3 and, 50 ppm GA3. The results showed that the seed dormancy was broken at 7 weeks after treatment, indicated by seed germination of >80%. The ABA levels of the seeds declined from week 0 to week 11, and the seed ABA and GA reached a balance from week 7 to week 11. Seeds treated with KNO3 or distilled water break dormancy after 8 weeks. The most effective treatment to break seed dormancy is the use of exogenous hormone GA3 at 50 ppm, with seed germination of >80% at week 5.
Co-Authors , Dina , Ramlafatma ,, Saipulloh . Giyanto Abdjad Asih Nawangsih Abdul Qadir Achmad Achmad Anak Agung Keswari Krisnandika Anggraini, Dwi Vista Annisa Nursoleha Ansella, Yohanda Anugrah, Mertya Arif, Mohamad Awang Maharijaya Bambang Sapta Purwoko Baran Wirawan Blair Moses Kamanga Cici Tresniawati Dahlan, Ade Muhamad Daniel Happy Putra Danu Kuncoro Devi Liana Devi Rusmin DEVI RUSMIN DEVI RUSMIN Didy Sopandie Dirgahani Putri Dwi Gery Febriyan Dwi Guntoro Dyah - Manohara Dyah Manohara DYAH MANOHARA Edi Santosa Edi Santosa Edi Santosa Edy Suprianto Efendi, Darda Endah Retno Palupi Endah Retno Palupi ENDANG MURNIATI Esti Rahayu Esty Puri UTAMI Esty Putri Utami Esty Putri Utami Faiza C. Suwarno Faiza C. Suwarno Faiza Chairani Suwarno Febriani, Lilis Yati Febriyan, Dwi Gery Gani Jawak Giyanto Giyanto Giyanto Giyanto Giyanto, dan Gundala, Belangie Tuahte Hadi, Putra K Haura Fikriyah Hakimah Herliyana Indahwardani Imroatus Sa’adah Indahwardani, Herliyana Iswari Saraswati Dewi Juliana Maia Kamanga, Blair Moses Kikin H Mutaqin Kresnamurti Kurniasih Krisnandika, A. A. Keswari Kusumastuti, Hamiddah Intan Kusumastuti, Shinta Nugraheni Lilis Yati Febriani M.R. SUHARTANTO M.R. SUHARTANTO, M.R. Maisaroh Tanjung MANOHARA, DYAH Manohara, Dyah - Maryati Sari Masitadewi, Ervin Mathius, dan Nurita Toruan Meity Suradji Sinaga Memen Surahman Mia Kosmiatin Mohamad Arif Mohamad Arif Mohamad Rahmad Suhartanto Mubarak, Farahdina Muhamad Rezki Mauladi Muhamad Syukur Muhammad Afif Muhammad Machmud Nadiya Iftiwata Rahmah Nelly Fridayanti Ni Made Armini Wiendi Nizaruddin . Norul Dewi Susanti Nur Syam Ismaniza A., Siti Nur Syam Ismaniza A. Nurita TORUAN-MATHIUS Nutrita Toruan Mathius Okti Syah Isyani Permatasari Peni Handayani Permatasari, Okti Syah Isyani Pitri Ratna Asih Pradana, Adhitya Vishnu Pramudita, Listya Purwoko, Bambang Sapta Purwoko Putra K Hadi Putri Mian Hairani Putro, Trisno Yuwono Qadir , Abdul Qadir, Abdul Qudus Sabha Adhinugraha R Pelealu, Rian Virvian Hidayat R. Palupi, Endah Rafi Fauzan Raga, Yuan Rahmah, Nadiya Iftiwa Riski Meliya Ningsih RUSMIN, DEVI RUSMIN, DEVI Sabar Pramono Sani, Mutiyara Sarah Desmia Muchtar Sarah Desmia Muchtar Satrias Ilyas Satriyas Ilyas SATRIYAS ILYAS Selly Salma Selly Salma Shinta Nugraheni Kusumastuti Siti Fadhilah, Siti Sobir Sobir Sri Suharyati Vityaningsih Sri Wilarso Budi SUHARTANTO, M.R. Suhartanto, Muhammad Rahmad Sukartini Supijatno Surya Kurnia Putra, Dicky Suwarno, Faiza C. Tanjung, Maisaroh Tantri Palupi Tati Budiarti Tefa, Anna Tefa, Anna Tri Astuti Trikoesoemaningtyas Trisno Yuwono Putro Wawan Hermawan Willy Bayuardi Suwarno Yuyuk Agung Lastiandika Yuyuk Agung Lastiandika