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Seed Coating with Biological Agent to Increace Plant Growth and Yield of Rice Palupi, Tantri; Ilyas, Satrias; Machmud, Muhammad; Widajati, Eny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 41 No. 3 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (309.365 KB) | DOI: 10.24831/jai.v41i3.7738

Abstract

Seed quality is an important factor in rice production. Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is a seedborne disease of rice that cause serious yield losses in Indonesia. Experiments were conducted at the Laboratory of Rice Research Babakan, IPB, Bogor; Laboratory of Seed Science and Technology at IPB, Bogor; and the Laboratory of Seed PT. EWSI, Purwakarta, from April to August 2011. The objective of this research was to determine the effectiveness of coating Xoo contaminated rice seeds with biological agents on plant growth and crop yield. The experiments were arranged in a randomized complete block design with one factor (seed coating treatment),i.e. T0 = negative control, healthy seed; T1 = positive control, the seeds contaminated with Xoo; T2 = P. diminuta A6 and B. subtilis 5/B; T3 = alginate 3% + 1% peat + P. diminuta A6 and B. subtilis % 5/B; T4 = arabic gum 3% + 1% gypsum + P. diminuta A6 and B. subtilis 5/B; T5 = CMC 1.5% + 1% talc + P. diminuta A6 and B. subtilis 5/B, and T6 = bactericide streptomycin sulfat 4%. The results showed that seed coating treatment with with 3% alginate + 1% peat + P. diminuta A6 + B. subtilis 5/B was the best treatment based on the percentage of full and empty grain weight per panicle, and the percentage of number of full and empty grains per panicle.
Seed Coating Sebagai Pengganti Fungsi Polong pada Penyimpanan Benih Kacang Tanah Sari, Maryati; Widajati, Eny; Asih, Pitri Ratna
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 41 No. 3 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (261.872 KB) | DOI: 10.24831/jai.v41i3.8099

Abstract

ABSTRAKPenelitian ini bertujuan untuk mencari alternatif pengganti fungsi polong dalam melindungi viabilitas benih kacangtanah selama penyimpanan, sehingga dapat menekan volume dan bobot dalam penyimpanan dan distribusi. Penelitiandilakukan pada benih kacang tanah varietas Kelinci. Percobaan dilaksanakan dengan rancangan petak terbagi. Petakutama adalah periode simpan, yaitu 0, 4, 7, 10, 13, dan 16 minggu. Anak petak adalah perlakuan pelapisan benih, yangterdiri atas benih dalam polong, benih kupas tanpa coating, benih kupas dengan coating arabic gum, coating arabic gum+ 0.5 g benomil L, coating arabic gum + 100 ppm, 150 ppm, dan 200 ppm tepung kurkuma, coating arabic gum + asamaskorbat 150 ppm, 250 ppm, dan 350 ppm. Hasil penelitian menunjukkan perlakuan coating + 0.5 g benomil L-1  danperlakuan coating + 350 ppm asam askorbat mampu mempertahankan daya berkecambah dan indeks vigor terbaik selamapenyimpanan. Kedua perlakuan tersebut nyata memberikan nilai indeks vigor yang lebih baik (setelah disimpan selama 16minggu, masing-masing memiliki nilai indeks vigor 40.2% dan 45.8%) dibandingkan perlakuan benih kupas tanpa coating(32.9%) dan perlakuan penyimpanan benih dalam polong (28.2%).Kata kunci: Arachis hypogaea L., pelapisan benih, penyimpanan benih, viabilitas benih
Metode Deteriorasi Terkontrol untuk Pendugaan Daya Simpan Benih Kedelai ., Nizaruddin; Suwarno, Faiza C.; Widajati, Eny; Qadir, Abdul
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 1 (2014): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (555.291 KB) | DOI: 10.24831/jai.v42i1.8145

Abstract

ABSTRACTPhysiological quality of soybean seeds was easily deteriorated during storage. The objectives of the research were to determine controlled deterioration test which could be used to evaluate soybean seed viability and to find the appropriate controlled deterioration test for estimating storability of soybean seed. The research was conducted at the quality control laboratory PT. BISI International, Tbk., Kediri since October 2012 until February 2013. The completely randomized design with four replications was used in the experiment by moisture content and deterioration periods as treatment. The results showed that the controlled deterioration periods to evaluate two varieties of soybean seed were same for 16 hours at 17.5% moisture content for Wilis and 15% moisture content for Detam-1. The result also showed that there were significant correlations between controlled deterioration values with actual values from 16 weeks storage periods, indicating controlled deterioration test periods could estimate storage periods. Verification of seed vigor relation to storability model showed there were no significant differences between germination and speed of germination between controlled deterioration and actual values. Simulation of soybean seed storability model with germination percentage after controlled deterioration as input variables could predict the storage periods.Keywords: Glycine max, rapid ageing, seed modelling, seed vigour, vigour index
Perubahan Fisik, Fisiologi dan Biokimia Selama Pemasakan Benih dan Studi Rekalsitransi Benih Kemiri Sunan Tresniawati, Cici; Murniati, Endang; Widajati, Eny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 1 (2014): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (352.208 KB) | DOI: 10.24831/jai.v42i1.8157

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ABSTRACTThe aims of this research on Reutealis trisperma seed were to know 1) seed harvest maturity level based on physiological and biochemical changes; and 2) seed recalcitrancy based on critical moisture content and storability in two storage temperatures. The research was conducted at Leuwikopo Seed Science and Technology Laboratory, from February 2012 to January 2013. There were three experiments, i.e. I. Effect of seed maturity level on seed viability and vigor (randomized completely block design, 3 seed maturity level, 3 replications); II. Determination of seed critical moisture content, used two methods, i.e. 1) fan drying and 2) air drying (completely randomized design in each drying methods, 3 replications); III. Effect of temperature and period of storage on seed viability and vigor (nested design, first factor was 2 storage temperature and the second factor was 7 storage periods, 3 replications). The result showed that seed physiological maturity achieved in 28 weeks after flowering with morphological criteria were brownish fruit, soft fruit exocarp, brown seed testa; physiological criteria was germination percentage 76-80% and biochemical criteria was carotenoid content 0.62 µmol(g FW)-1. Carotenoid content can be used as biochemical indicator to determine fruit maturity and significantly correlated with moisture content, seed dry weight and germination percentage. Critical moisture content were 8.2-10.9%. These data suggested that seeds could be classified as intermediate. R. trisperma  seeds could be stored for three weeks in temperature 19-28 oC and RH 50-70%, with 12% moisture content used polypropilen plastic bag Keywords: carotenoid, critical moisture content, desiccation, Reutealis trisperma
Efektivitas Bahan Pelapis Benih terhadap Penyerapan Fosfat dan Pertumbuhan Bibit Kelapa Sawit ,, Saipulloh; Palupi, Endah Retno; Widajati, Eny; Mathius, dan Nurita Toruan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 1 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (350.151 KB) | DOI: 10.24831/jai.v45i1.12236

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One way of overcome phosphate deficiency in oil palm plantation is seed enrichment using phosphate solvent microbes. This technique is expected to accelerate phosphate availability for plant. The aim of the research was to identify the best coating materials for seed enrichment that compatible with Burkholderia sp. in order to increase phosphate absorption, seed storability, and growth of oil palm seedlings. This research consisted of two consecutive experiments. The first experiment was arranged in randomized block design, with nine treatments of different seed coating materials. The second experiment was arranged in nested design, with main factor of 11 different seed coating materials and nested factors were five storage periods. The data was collected on growth parameters of seedling height, root length, trunk height, dry weight; phosphate absorption, and DGGE analysis. The research showed there were three best seed coating materials of CMC 1.5%, CMC 2% + gypsum 1.5%, and CMC 1.5% + talc 1%. The coating material enhances seedling growth and phosphate absorption. Seed coating and enrichment maintained seedling vigor for three days in the storage. Keywords: Burkholderia sp., carboxyl methyl cellulose, phosphate solvent microbial, seed enrichment, seed storage
Peningkatan Mutu Fisiologis dan Daya Simpan Benih serta Ketahanan Patogen dan Agen Hayati pada Benih Padi Berpelapis Palupi, Tantri; Ilyas, Satriyas; Machmud, Muhammad; Widajati, dan Eny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 3 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.508 KB) | DOI: 10.24831/jai.v44i3.12755

Abstract

ABSTRACTXanthomonas oryzae pv. oryzae (Xoo), is a seedborne pathogen causing bacterial leaf blight (BLB) disease, and reduces the quality of seed and rice production. One of the efforts to control the BLB disease and to improve the quality Xoo infected seeds is the seed coating technique enriched with biological agents. The experiment was aimed to study the effect of coating on seed quality and storage life, as well as the Xoo and biological agents resistence (P. diminuta A6 and B. subtilis 5/B) on the seeds. The experiment was carried out from August 2011 to March 2012, using a split plot design with four replications. The main plot was storage period, i.e. 0, 1, 2, 3, 4, 5, 6, and 7 months. The sub plot was seed coating treatment consisted of negative control (healthy seed); positive control (seeds contaminated with Xoo); seed infested with biological agens;  alginate 3% + 1% peat + biological agents; arabic gum 3% + 1% gypsum + biological agents; CMC 1.5% + 1% talc + biological  agents;  and  bactericide  streptomycin  sulfat 20%. The  coated  seeds were  stored an air-conditioned room (18-20 °C,  RH  48-50%). The results showed that the treatments were able to maintain seeds quality during storage, i.e. germination percentage, uniformity percentage, and vigor index, better than those of the positive control. The P. diminuta A6 was still presence (0.08 x 106 cfu mL-1) in seeds coated after 7 month storage, and the B. subtilis 5/B was still presence (0.07 x 106 cfu mL-1) up to 6 month storage with 3% arabic gum + 1% gypsum + biological agents.  Keywords: Bacillus subtilis 5/B, Pseudomonas diminuta A6, seed quality, storage space, Xanthomonas oryzae pv. oryzae
Aplikasi Bakteri Probiotik untuk Meningkatkan Mutu Fisiologi dan Kesehatan Bibit Cabai (Capsicum annuum L.) Tefa, Anna; Widajati, Eny; Syukur, Muhamad; Giyanto, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 2 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.829 KB) | DOI: 10.24831/jai.v44i2.13487

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ABSTRACTThe use of probiotic bacteria as biocontrol agents is one of the methods of controlling anthracnose disease caused by Colletotrichum acutatum. The objective of this research was to suppress the infection of C. acutatum and increase chilli pepper seedling vigour. The research involved factorial experiments arranged in a completely randomized design with two factors. The first factor was seed coating involving six treatments, i.e., control, seed coating without bacteria, seed coating with Bacillus sp., Pseudomonas sp., Actinomycetes sp, and fungicide. The second factor was the seed storage period where six storage periods were experimented, i.e., 0, 1, 2, 3, 4, 5, and 6 months. The results showed that the coating treatment of Bacillus sp., Pseudomonas sp., and Actinomycetes sp. improved germination, growth rate and number of leaves and reduced the incidence of attacks and infection of hypocotyls at 5 month storage period.Keywords: Actinomycetes sp., Bacillus sp., Pseudomonas sp., seed coating, storage period
Aplikasi Bakteri Probiotik Pseudomonas Kelompok Fluorescens untuk Meningkatkan Produksi dan Mutu Benih Cabai Permatasari, Okti Syah Isyani; Widajati, Eny; Syukur, Muhamad; Giyanto, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 3 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (399.261 KB) | DOI: 10.24831/jai.v44i3.13544

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ABSTRACTThe use of high quality seed is one of the key factors to improve productivity. Probiotic bacteria has been used to increase plant growth and to control pathogens. The objective of the research was to evaluate methods of chili seed production that yielded high physiological and pathological quality using probiotic fluorescent bacteria Pseudomonas (P24). The bacteria was expected to function as plant growth promoting bacteria as well as capable of controling seedborne pathogen Colletotrichum acutatum causes antrachnose. The experiment was conducted during March until October 2014 in Seed Health Laboratory, Plant Bacteriology Laboratory, and Leuwikopo experimental garden, IPB. The experiment was arranged in a split plot randomized complete block design with three replications. The main plots were untreated and inoculation of C. acutatum. The subplot was six treatments of fluorescent Pseudomonas (P24) application. The results showed that matriconditioning and spraying of fluorescent Pseudomonas (P24) on nursery decreased disease incidence significantly. Matriconditioning and spraying of fluorescent Pseudomonas (P24) on nursery and flowering phase increased number of healthy fruit and total weight of seeds per plant significantly. These applications also increased seed physiological  quality indicated by germination percentage (GP) 77.04%, growth rate (GR) 9.72% etmal-1, vigor index (VI) 29.74%, and seed health by suppresed C. acutatum infection up to 12.25%.Keywords: anthracnose, Colletotrichum acutatum, control, matriconditioning, spraying
Karakteristik Morfologi Benih sebagai Parameter untuk Penentuan Pohon Induk Sumber Benih Pala (Myristica fragrans Houtt.) Pramudita, Listya; Widajati, Eny; Suwarno, Faiza Chairani; Surahman, dan Memen
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 1 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (510.862 KB) | DOI: 10.24831/jai.v45i1.13755

Abstract

Nutmeg is an Indonesian native plant, produces essential oill as an export commodity. However, it has low seed uniformity that mengurangi keseragaman tanaman di lapang. The main objective of this research was to obtain morphological characteritics as parameter for nutmeg mother plants selection. This field research was carried out from August-December 2014 at Toisapu, Wakal and Lula nutmeg seed source plantations in Ambon, and Seed Storage and Testing Laboratory, IPB. Characterization was undertaken on ten mother plants from each  Wakal and Lula seed source plantations and eight mother plants from Toisapu seed source plantation. Quantitative and qualitative characters of vegetative, fruit, mace and seed from every mother plants were analyzed with  Gower’s similarity test. Mother plants that produced high uniformity seed was the 7th (0.80), the 8th (0.93) and the 10th (0.80) mother plants in Wakal; the 2nd (0.80) mother plant in Toisapu; the  2nd (0.82), the 5th (0.80), the 6th (0.82), the 8th (0.84), the 9th (0.80) and the 10th (0.93)  mother plants in Lula. Those selected mother plants produced morphological uniform seed morphologically. Lula seed plantation had the highest uniformity level among mother plants (0.77-0.87) compared to Toisapu (0.70-0.87) and Wakal (0.69-0.84) mother plants. The morphological characteristic can be use to select the source seed mother plants which able to produce high morphological uniformity seeds.Keywords: Gower’s similarity, seed production, uniformity
Kualitas Benih Berdasarkan Warna Kulit dan Bagian Buah, serta Kualitas Bibit Srikaya dengan Pemberian PGPR dan CaCO3 Riski Meliya Ningsih; Widajati, Eny; Endah Retno Palupi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 49 No. 2 (2021): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1036.146 KB) | DOI: 10.24831/jai.v49i2.36381

Abstract

Benih srikaya yang bermutu sangat diperlukan untuk mendukung pengembangan bibit tanaman srikaya. Penelitian ini bertujuan menentukan tingkat kemasakan dan posisi benih dalam buah yang tepat guna mendapatkan benih bermutu dan mendapatkan perlakuan terbaik untuk meningkatkan performa bibit melalui penggunaan rizobakteri dan CaCO3. Percobaan pertama menggunakan rancangan acak lengkap (RAL) dua faktor dengan faktor pertama adalah warna kulit buah dan faktor kedua adalah posisi benih dalam buah. Percobaan kedua menggunakan RAL dengan rizobakteri, CaCO3, dan kombinasi keduanya sebagai faktor tunggal. Waktu hitungan pertama pengamatan daya berkecambah pada 36 hari setelah tanam (HST) dan hitungan akhir pada 56 HST. Kualitas benih terbaik diperoleh dari warna kulit buah kuning berdasarkan bobot kering benih, daya berkecambah, indeks vigor, bobot kering kecambah normal, dan kecepatan tumbuh benih. Vigor tumbuh benih tertinggi diperoleh dari buah dengan warna kulit kuning dari bagian ujung dan pangkal buah. Perlakuan CaCO3 mempengaruhi pemanjangan akar, lebar daun, diameter batang, dan panjang daun. Aplikasi CaCO3 memberikan pengaruh terbaik terhadap pertumbuhan bibit diikuti dengan perlakuan Pseudomonas + Actimomycetes + CaCO3. Kata kunci: Annona squamosa, batang bawah, hitungan akhir, indeks vigor, rizobakteri
Co-Authors , Dina , Ramlafatma ,, Saipulloh . Giyanto Abdjad Asih Nawangsih Abdul Qadir Abdul Qadir Abdul Qadir Abdul Qadir Achmad Achmad Adhitya Vishnu Pradana Aminatun Nisa Anak Agung Keswari Krisnandika ANDI NADIA NURUL LATHIFA HATTA Anggraini, Dwi Vista Ani Kurniawati Annisa Nursoleha Ansella, Yohanda Arif, Mohamad ARYA WIDURA RITONGA Awang Maharijaya Bambang Sapta Purwoko Baran Wirawan Blair Moses Kamanga Chusnul Arif Cici Tresniawati Dahlan, Ade Muhamad Daniel Happy Putra Danu Kuncoro Darda Efendi Devi Liana DEVI RUSMIN DEVI RUSMIN Devi Rusmin Diaguna, Ridwan Didy Sopandie Dirgahani Putri Dwi Gery Febriyan Dwi Guntoro Dyah - Manohara DYAH MANOHARA Dyah Manohara Edi Santosa Edi Santosa Edi Santosa Edy Suprianto ENDAH RETNO PALUPI Endah Retno Palupi Endah Retno Palupi ENDANG MURNIATI Esti Rahayu Esty Puri UTAMI Esty Putri Utami Esty Putri Utami Fahmi, Mochammad Faiza C. Suwarno Faiza C. Suwarno Faiza Chairani Suwarno Febriani, Lilis Yati Febriyan, Dwi Gery Fridayanti, Nelly GAGAD RESTU PRATIWI Gani Jawak Giyanto Giyanto Giyanto Giyanto Giyanto, dan Gundala, Belangie Tuahte Hadi, Putra K Hairani, Putri Mian Haura Fikriyah Hakimah Heni Purnamawati Herliyana Indahwardani Imroatus Sa’adah Indahwardani, Herliyana Irfan Firmansyah Iswari Saraswati Dewi Juliana Maia Kamanga, Blair Moses Kikin H Mutaqin Kresnamurti Kurniasih Krisnandika, A. A. Keswari Kusumastuti, Hamiddah Intan Kusumastuti, Shinta Nugraheni Lilis Yati Febriani M.R. SUHARTANTO M.R. SUHARTANTO, M.R. Maisaroh Tanjung MANOHARA, DYAH Manohara, Dyah - Maryati Sari Masitadewi, Ervin Mathius, dan Nurita Toruan Meity Suradji Sinaga Memen Surahman Mertya Anugrah Mia Kosmiatin Mohamad Arif Mohamad Arif Mohamad Rahmad Suhartanto Mubarak, Farahdina Muhamad Rezki Mauladi Muhamad Syukur Muhammad Afif Muhammad Imam Surya Muhammad Machmud MUHAMMAD MACHMUD Muhammad Machmud Muhammad Syukur Nadiya Iftiwata Rahmah Nelly Fridayanti Ni Made Armini Wiendi Nizaruddin . Norul Dewi Susanti Nur Syam Ismaniza A., Siti Nur Syam Ismaniza A. Nurita TORUAN-MATHIUS Nutrita Toruan Mathius Otih Rostiana Peni Handayani Permatasari, Okti Syah Isyani Pitri Ratna Asih Pramudita, Listya Putra K Hadi Putro, Trisno Yuwono Qadir , Abdul Qudus Sabha Adhinugraha R Pelealu, Rian Virvian Hidayat R. Palupi, Endah Rafi Fauzan Raga, Yuan Rahmah, Nadiya Iftiwa Raihan Anwar Thaha Riski Meliya Ningsih RUSMIN, DEVI RUSMIN, DEVI Sabar Pramono Sani, Mutiyara Sarah Desmia Muchtar Sarah Desmia Muchtar Satrias Ilyas SATRIYAS ILYAS Satriyas Ilyas SATRIYAS ILYAS SATRIYAS ILYAS Satriyas Ilyas Selly Salma Selly Salma Setyo Pertiwi Shinta Nugraheni Kusumastuti Silvan Timothy Siti Fadhilah, Siti Sobir Sobir Sri Suharyati Vityaningsih Sri Wilarso Budi SUHARTANTO, M.R. Sukartini Supijatno Surya Kurnia Putra, Dicky Suwarno, Faiza C. Tanjung, Maisaroh Tantri Palupi Tati Budiarti Tefa, Anna Tri Astuti Trikoesoemaningtyas Trisno Yuwono Putro Wawan Hermawan Willy Bayuardi Suwarno Yuyuk Agung Lastiandika Yuyuk Agung Lastiandika ZULFIKAR DAMARALAM SAHID