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Peningkatan Mutu Fisiologis dan Daya Simpan Benih serta Ketahanan Patogen dan Agen Hayati pada Benih Padi Berpelapis Palupi, Tantri; Ilyas, Satriyas; Machmud, Muhammad; Widajati, dan Eny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 3 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.508 KB) | DOI: 10.24831/jai.v44i3.12755

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ABSTRACTXanthomonas oryzae pv. oryzae (Xoo), is a seedborne pathogen causing bacterial leaf blight (BLB) disease, and reduces the quality of seed and rice production. One of the efforts to control the BLB disease and to improve the quality Xoo infected seeds is the seed coating technique enriched with biological agents. The experiment was aimed to study the effect of coating on seed quality and storage life, as well as the Xoo and biological agents resistence (P. diminuta A6 and B. subtilis 5/B) on the seeds. The experiment was carried out from August 2011 to March 2012, using a split plot design with four replications. The main plot was storage period, i.e. 0, 1, 2, 3, 4, 5, 6, and 7 months. The sub plot was seed coating treatment consisted of negative control (healthy seed); positive control (seeds contaminated with Xoo); seed infested with biological agens;  alginate 3% + 1% peat + biological agents; arabic gum 3% + 1% gypsum + biological agents; CMC 1.5% + 1% talc + biological  agents;  and  bactericide  streptomycin  sulfat 20%. The  coated  seeds were  stored an air-conditioned room (18-20 °C,  RH  48-50%). The results showed that the treatments were able to maintain seeds quality during storage, i.e. germination percentage, uniformity percentage, and vigor index, better than those of the positive control. The P. diminuta A6 was still presence (0.08 x 106 cfu mL-1) in seeds coated after 7 month storage, and the B. subtilis 5/B was still presence (0.07 x 106 cfu mL-1) up to 6 month storage with 3% arabic gum + 1% gypsum + biological agents.  Keywords: Bacillus subtilis 5/B, Pseudomonas diminuta A6, seed quality, storage space, Xanthomonas oryzae pv. oryzae
Aplikasi Bakteri Probiotik untuk Meningkatkan Mutu Fisiologi dan Kesehatan Bibit Cabai (Capsicum annuum L.) Tefa, Anna; Widajati, Eny; Syukur, Muhamad; Giyanto, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 2 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.829 KB) | DOI: 10.24831/jai.v44i2.13487

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ABSTRACTThe use of probiotic bacteria as biocontrol agents is one of the methods of controlling anthracnose disease caused by Colletotrichum acutatum. The objective of this research was to suppress the infection of C. acutatum and increase chilli pepper seedling vigour. The research involved factorial experiments arranged in a completely randomized design with two factors. The first factor was seed coating involving six treatments, i.e., control, seed coating without bacteria, seed coating with Bacillus sp., Pseudomonas sp., Actinomycetes sp, and fungicide. The second factor was the seed storage period where six storage periods were experimented, i.e., 0, 1, 2, 3, 4, 5, and 6 months. The results showed that the coating treatment of Bacillus sp., Pseudomonas sp., and Actinomycetes sp. improved germination, growth rate and number of leaves and reduced the incidence of attacks and infection of hypocotyls at 5 month storage period.Keywords: Actinomycetes sp., Bacillus sp., Pseudomonas sp., seed coating, storage period
Aplikasi Bakteri Probiotik Pseudomonas Kelompok Fluorescens untuk Meningkatkan Produksi dan Mutu Benih Cabai Permatasari, Okti Syah Isyani; Widajati, Eny; Syukur, Muhamad; Giyanto, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 3 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (399.261 KB) | DOI: 10.24831/jai.v44i3.13544

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ABSTRACTThe use of high quality seed is one of the key factors to improve productivity. Probiotic bacteria has been used to increase plant growth and to control pathogens. The objective of the research was to evaluate methods of chili seed production that yielded high physiological and pathological quality using probiotic fluorescent bacteria Pseudomonas (P24). The bacteria was expected to function as plant growth promoting bacteria as well as capable of controling seedborne pathogen Colletotrichum acutatum causes antrachnose. The experiment was conducted during March until October 2014 in Seed Health Laboratory, Plant Bacteriology Laboratory, and Leuwikopo experimental garden, IPB. The experiment was arranged in a split plot randomized complete block design with three replications. The main plots were untreated and inoculation of C. acutatum. The subplot was six treatments of fluorescent Pseudomonas (P24) application. The results showed that matriconditioning and spraying of fluorescent Pseudomonas (P24) on nursery decreased disease incidence significantly. Matriconditioning and spraying of fluorescent Pseudomonas (P24) on nursery and flowering phase increased number of healthy fruit and total weight of seeds per plant significantly. These applications also increased seed physiological  quality indicated by germination percentage (GP) 77.04%, growth rate (GR) 9.72% etmal-1, vigor index (VI) 29.74%, and seed health by suppresed C. acutatum infection up to 12.25%.Keywords: anthracnose, Colletotrichum acutatum, control, matriconditioning, spraying
Karakteristik Morfologi Benih sebagai Parameter untuk Penentuan Pohon Induk Sumber Benih Pala (Myristica fragrans Houtt.) Pramudita, Listya; Widajati, Eny; Suwarno, Faiza Chairani; Surahman, dan Memen
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 1 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (510.862 KB) | DOI: 10.24831/jai.v45i1.13755

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Nutmeg is an Indonesian native plant, produces essential oill as an export commodity. However, it has low seed uniformity that mengurangi keseragaman tanaman di lapang. The main objective of this research was to obtain morphological characteritics as parameter for nutmeg mother plants selection. This field research was carried out from August-December 2014 at Toisapu, Wakal and Lula nutmeg seed source plantations in Ambon, and Seed Storage and Testing Laboratory, IPB. Characterization was undertaken on ten mother plants from each  Wakal and Lula seed source plantations and eight mother plants from Toisapu seed source plantation. Quantitative and qualitative characters of vegetative, fruit, mace and seed from every mother plants were analyzed with  Gower’s similarity test. Mother plants that produced high uniformity seed was the 7th (0.80), the 8th (0.93) and the 10th (0.80) mother plants in Wakal; the 2nd (0.80) mother plant in Toisapu; the  2nd (0.82), the 5th (0.80), the 6th (0.82), the 8th (0.84), the 9th (0.80) and the 10th (0.93)  mother plants in Lula. Those selected mother plants produced morphological uniform seed morphologically. Lula seed plantation had the highest uniformity level among mother plants (0.77-0.87) compared to Toisapu (0.70-0.87) and Wakal (0.69-0.84) mother plants. The morphological characteristic can be use to select the source seed mother plants which able to produce high morphological uniformity seeds.Keywords: Gower’s similarity, seed production, uniformity
Pengaruh Formula Coating terhadap Viabilitas dan Vigor serta Daya Simpan Benih Padi (Oryza sativa L.) Palupi, Tantri; Ilyas, Satriyas; Machmud, Muhammad; Widajati, Eny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 40 No. 1 (2012): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (306.517 KB) | DOI: 10.24831/jai.v40i1.14255

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The objectives of this research were to find the most compatible coating formula for rice seeds (Experiment 1), and to evaluate the effect of coating formula on viability, vigor and storability of the seeds (Experiment 2). This research was conducted in Laboratory of Seed Science and Technology at IPB, Bogor and Laboratory of Seed Technology at PT East West Seed Indonesia, Purwakarta from December 2010 to February 2011. In Experiment 1, the ten coating materials (formula) were arranged in single factor of Completely Randomized Design with four replications, i.e. uncoated seed, 3% alginate + 1% talc, 3% alginate + 1% gypsum, 3% alginate + 1% peat, 3% arabic gum + 1% talc, 3% arabic gum + 1% gypsum, 3% arabic gum + 1% peat, 1.5% CMC + 1% talc, 1.5% CMC + 1% gypsum, and 1.5% CMC + 1% peat. Experiment 2 used Split Plot Design with four replications. The main plot was storage periods i.e. 0, 1 and 2 months. The seeds were stored in two storage conditions: ambient and air conditioned room. The subplot was coating formula (same as in Experiment 1). The results of Experiment 1 showed that the most compatible coating material for rice seeds were 1.5% CMC + 1% talc, and 1.5% CMC + 1% gypsum, these formula were able to maintain vigor of the coated seeds. In Experiment 2, coating formula of 1.5% CMC + 1% peat generated higher seed vigor than uncoated seeds after two months storage, 3% gum arabic + 1% gypsum was able to keep vigor for one month storage in ambient condition. The coating formula of 3% alginate + 1% peat was able to keep viability and vigor of the coated seeds for two months in air conditioned room. Keywords: seed coating material, seed quality, seed storage condition, seed storability
Kualitas Benih Berdasarkan Warna Kulit dan Bagian Buah, serta Kualitas Bibit Srikaya dengan Pemberian PGPR dan CaCO3 Riski Meliya Ningsih; Widajati, Eny; Endah Retno Palupi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 49 No. 2 (2021): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1036.146 KB) | DOI: 10.24831/jai.v49i2.36381

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Benih srikaya yang bermutu sangat diperlukan untuk mendukung pengembangan bibit tanaman srikaya. Penelitian ini bertujuan menentukan tingkat kemasakan dan posisi benih dalam buah yang tepat guna mendapatkan benih bermutu dan mendapatkan perlakuan terbaik untuk meningkatkan performa bibit melalui penggunaan rizobakteri dan CaCO3. Percobaan pertama menggunakan rancangan acak lengkap (RAL) dua faktor dengan faktor pertama adalah warna kulit buah dan faktor kedua adalah posisi benih dalam buah. Percobaan kedua menggunakan RAL dengan rizobakteri, CaCO3, dan kombinasi keduanya sebagai faktor tunggal. Waktu hitungan pertama pengamatan daya berkecambah pada 36 hari setelah tanam (HST) dan hitungan akhir pada 56 HST. Kualitas benih terbaik diperoleh dari warna kulit buah kuning berdasarkan bobot kering benih, daya berkecambah, indeks vigor, bobot kering kecambah normal, dan kecepatan tumbuh benih. Vigor tumbuh benih tertinggi diperoleh dari buah dengan warna kulit kuning dari bagian ujung dan pangkal buah. Perlakuan CaCO3 mempengaruhi pemanjangan akar, lebar daun, diameter batang, dan panjang daun. Aplikasi CaCO3 memberikan pengaruh terbaik terhadap pertumbuhan bibit diikuti dengan perlakuan Pseudomonas + Actimomycetes + CaCO3. Kata kunci: Annona squamosa, batang bawah, hitungan akhir, indeks vigor, rizobakteri
Seleksi Galur-galur Dihaploid Padi Hitam pada Uji Daya Hasil Lanjutan Kuncoro, Danu; Sani, Mutiyara; Purwoko, Bambang Sapta Purwoko; Dewi, Iswari Saraswati; Suwarno, Willy Bayuardi; Maharijaya, Awang; Widajati, Eny; Bambang Sapta Purwoko
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 49 No. 3 (2021): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.021 KB) | DOI: 10.24831/jai.v49i3.37179

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Padi hitam merupakan sumber karbohidrat yang bermanfaat untuk kesehatan. Pengujian daya hasil merupakan tahapan penting dalam menentukan keberhasilan pemuliaan padi beras hitam. Tujuan penelitian ini ialah untuk memperoleh informasi keragaan agronomi dan menyeleksi galur-galur dihaploid padi beras hitam yang memiliki penampilan agronomi baik dan berdaya hasil tinggi untuk pengujian multi lokasi. Penelitian ini dilakukan dari bulan November 2019 sampai Juli 2020 di Bogor, Jawa Barat dan Malang, Jawa Timur. Sebanyak 23 galur dihaploid padi hitam yang memiliki sifat agronomi baik dengan produktivitas tinggi dan 3 varietas pembanding yaitu Aek Sibundong, Jeliteng, dan Inpari 24 digunakan sebagai materi genetik. Studi ini menggunakan rancangan rancangan kelompok lengkap teracak dengan 3 ulangan pada setiap percobaan. Hasil penelitian menunjukkan bahwa interaksi genotipe dan lingkungan berpengaruh nyata terhadap semua karakter pengamatan kecuali pada karakter lama pengisian gabah berdasarkan analisis keragaman. Sebanyak 14 galur yang terseleksi berdasarkan indeks terboboti memiliki karakteristik jumlah anakan produktif per rumpun dari 15.8 hingga 25.9, umur panen genjah dari 115.2 to 121.9 hari setelah semai, dan produktivitas >5.4 ton ha-1. Keragaan agronomi dan daya hasil galur-galur dihaploid padi yang terseleksi pada penelitian ini memerlukan pengujian lebih lanjut melalui uji multi lokasi untuk memperoleh calon varietas unggul baru padi beras hitam. Kata kunci: agronomi baik, indeks seleksi, kriteria seleksi, kultur antera padi
Seed invigoration using ultrafine bubble water to increase the vigor of true shallot seed (Allium ascalonicum L.) Raga, Yuan; Widajati, Eny; Purwanto, Yohanes Aris
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 51 No. 1 (2023): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (303.794 KB) | DOI: 10.24831/ija.v51i1.43000

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True shallot seeds are preferable to tubers as planting materials because of their advantages in storage longevity, lower management cost, and lower seed-borne disease risk. However, environmentally limited production and seed deterioration in uncontrolled storage have limited its application. Pre-sowing hydration invigoration accelerates and synchronizes the germination of deteriorated seeds. Ultra-fine bubble water (UFBW) contains reactive oxygen species, which can advance the pre-germinative metabolism of the seed. This study examined true shallot seed invigoration using UFBW. The experiment was conducted in a two-factor of randomized complete block design. The first factor was initial seed vigor based on the vigor index with six levels, i.e., 24, 15, 13, 12, 10, and 9%. The second factor was the invigoration treatment of UFBW (8 and 23 ppm dissolved oxygen concentrations) with two durations (24 and 48 h), 50 ppm GA3, and 3% KNO3 (24 h). UFBW with 23 ppm invigoration for 48 h improved seed vigor based on all vigor parameters. The GA3 50 ppm treatment is the most effective in increasing seed vigor. Keywords: dissolved oxygen; pre-sowing hydration; reactive oxygen species; seed viability; seed vigor
Pengembangan Metode Uji Tetrazolium dengan Sensor Infra red sebagai Uji Viabilitas pada Benih Kacang Tanah (Arachis hypogea L.) Widajati, Eny; Ansella, Yohanda; Dahlan, Ade Muhamad; Putro, Trisno Yuwono; Masitadewi, Ervin
Buletin Agrohorti Vol. 12 No. 2 (2024): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrob.v12i2.54759

Abstract

Pengujian viabilitas benih menggunakan uji tetrazolium merupakan salah satu metode penentuan viabilitas benih dengan mengevaluasi tingkat warna merah yang terbentuk pada benih. Warna merah terbentuk dari aktivitas enzim dehidrogenase pada siklus trichloroacetic acid. Penentuan tingkat warna merah pada benih ini dapat menggunakan alat sensor infra red. Penelitian ini bertujuan menguji alat sensor infra red untuk menentukan intensitas warna secara kuantitatif dalam uji tetrazolium serta menentukan korelasi alat sensor infra red dengan pengujian benih secara fisiologis. Percobaan disusun menggunakan rancangan kelompok lengkap teracak dengan faktor tunggal yaitu tingkat viabilitas benih berdasarkan daya berkecambah masing-masing adalah 78.9%, 70.7% dan 40.0 %. Hasil penelitian menunjukkan bahwa aplikasi sensor intensitas infra red untuk uji tetrazolium pada benih kacang tanah menghasilkan nilai koefisien korelasi (r) sebesar 0.62 antara nilai RGB merah bagian dalam kotiledon (Rd) dengan kecambah normal. Kata kunci: daya berkecambah, enzim dehydrogenase, kotiledon, mutu fisiologis benih, RGB
Co-Authors , Dina , Ramlafatma ,, Saipulloh . Giyanto Abdjad Asih Nawangsih Abdul Qadir Achmad Achmad Anak Agung Keswari Krisnandika Anggraini, Dwi Vista Annisa Nursoleha Ansella, Yohanda Anugrah, Mertya Arif, Mohamad Awang Maharijaya Bambang Sapta Purwoko Baran Wirawan Blair Moses Kamanga Cici Tresniawati Dahlan, Ade Muhamad Daniel Happy Putra Danu Kuncoro Devi Liana DEVI RUSMIN DEVI RUSMIN Devi Rusmin Didy Sopandie Dirgahani Putri Dwi Gery Febriyan Dwi Guntoro Dyah - Manohara DYAH MANOHARA Dyah Manohara Edi Santosa Edi Santosa Edi Santosa Edy Suprianto Efendi, Darda Endah Retno Palupi Endah Retno Palupi ENDANG MURNIATI Esti Rahayu Esty Puri UTAMI Esty Putri Utami Esty Putri Utami Faiza C. Suwarno Faiza C. Suwarno Faiza Chairani Suwarno Febriani, Lilis Yati Febriyan, Dwi Gery Gani Jawak Giyanto Giyanto Giyanto Giyanto Giyanto, dan Gundala, Belangie Tuahte Hadi, Putra K Haura Fikriyah Hakimah Herliyana Indahwardani Imroatus Sa’adah Indahwardani, Herliyana Iswari Saraswati Dewi Juliana Maia Kamanga, Blair Moses Kikin H Mutaqin Kresnamurti Kurniasih Krisnandika, A. A. Keswari Kusumastuti, Hamiddah Intan Kusumastuti, Shinta Nugraheni Lilis Yati Febriani M.R. SUHARTANTO M.R. SUHARTANTO, M.R. Maisaroh Tanjung MANOHARA, DYAH Manohara, Dyah - Maryati Sari Masitadewi, Ervin Mathius, dan Nurita Toruan Meity Suradji Sinaga Memen Surahman Mia Kosmiatin Mohamad Arif Mohamad Arif Mohamad Rahmad Suhartanto Mubarak, Farahdina Muhamad Rezki Mauladi Muhamad Syukur Muhammad Afif Muhammad Machmud Nadiya Iftiwata Rahmah Nelly Fridayanti Ni Made Armini Wiendi Nizaruddin . Norul Dewi Susanti Nur Syam Ismaniza A., Siti Nur Syam Ismaniza A. Nurita TORUAN-MATHIUS Nutrita Toruan Mathius Okti Syah Isyani Permatasari Peni Handayani Permatasari, Okti Syah Isyani Pitri Ratna Asih Pradana, Adhitya Vishnu Pramudita, Listya Purwoko, Bambang Sapta Purwoko Putra K Hadi Putri Mian Hairani Putro, Trisno Yuwono Qadir , Abdul Qadir, Abdul Qudus Sabha Adhinugraha R Pelealu, Rian Virvian Hidayat R. Palupi, Endah Rafi Fauzan Raga, Yuan Rahmah, Nadiya Iftiwa Riski Meliya Ningsih RUSMIN, DEVI RUSMIN, DEVI Sabar Pramono Sani, Mutiyara Sarah Desmia Muchtar Sarah Desmia Muchtar Satrias Ilyas Satriyas Ilyas SATRIYAS ILYAS Selly Salma Selly Salma Shinta Nugraheni Kusumastuti Siti Fadhilah, Siti Sobir Sobir Sri Suharyati Vityaningsih Sri Wilarso Budi SUHARTANTO, M.R. Suhartanto, Muhammad Rahmad Sukartini Supijatno Surya Kurnia Putra, Dicky Suwarno, Faiza C. Tanjung, Maisaroh Tantri Palupi Tati Budiarti Tefa, Anna Tefa, Anna Tri Astuti Trikoesoemaningtyas Trisno Yuwono Putro Wawan Hermawan Willy Bayuardi Suwarno Yuyuk Agung Lastiandika Yuyuk Agung Lastiandika