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Seleksi Galur-galur Dihaploid Padi Hitam pada Uji Daya Hasil Lanjutan Kuncoro, Danu; Sani, Mutiyara; Purwoko, Bambang Sapta Purwoko; Dewi, Iswari Saraswati; Suwarno, Willy Bayuardi; Maharijaya, Awang; Widajati, Eny; Bambang Sapta Purwoko
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 49 No. 3 (2021): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.021 KB) | DOI: 10.24831/jai.v49i3.37179

Abstract

Padi hitam merupakan sumber karbohidrat yang bermanfaat untuk kesehatan. Pengujian daya hasil merupakan tahapan penting dalam menentukan keberhasilan pemuliaan padi beras hitam. Tujuan penelitian ini ialah untuk memperoleh informasi keragaan agronomi dan menyeleksi galur-galur dihaploid padi beras hitam yang memiliki penampilan agronomi baik dan berdaya hasil tinggi untuk pengujian multi lokasi. Penelitian ini dilakukan dari bulan November 2019 sampai Juli 2020 di Bogor, Jawa Barat dan Malang, Jawa Timur. Sebanyak 23 galur dihaploid padi hitam yang memiliki sifat agronomi baik dengan produktivitas tinggi dan 3 varietas pembanding yaitu Aek Sibundong, Jeliteng, dan Inpari 24 digunakan sebagai materi genetik. Studi ini menggunakan rancangan rancangan kelompok lengkap teracak dengan 3 ulangan pada setiap percobaan. Hasil penelitian menunjukkan bahwa interaksi genotipe dan lingkungan berpengaruh nyata terhadap semua karakter pengamatan kecuali pada karakter lama pengisian gabah berdasarkan analisis keragaman. Sebanyak 14 galur yang terseleksi berdasarkan indeks terboboti memiliki karakteristik jumlah anakan produktif per rumpun dari 15.8 hingga 25.9, umur panen genjah dari 115.2 to 121.9 hari setelah semai, dan produktivitas >5.4 ton ha-1. Keragaan agronomi dan daya hasil galur-galur dihaploid padi yang terseleksi pada penelitian ini memerlukan pengujian lebih lanjut melalui uji multi lokasi untuk memperoleh calon varietas unggul baru padi beras hitam. Kata kunci: agronomi baik, indeks seleksi, kriteria seleksi, kultur antera padi
Seed invigoration using ultrafine bubble water to increase the vigor of true shallot seed (Allium ascalonicum L.) Raga, Yuan; Widajati, Eny; Purwanto, Yohanes Aris
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 51 No. 1 (2023): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (303.794 KB) | DOI: 10.24831/ija.v51i1.43000

Abstract

True shallot seeds are preferable to tubers as planting materials because of their advantages in storage longevity, lower management cost, and lower seed-borne disease risk. However, environmentally limited production and seed deterioration in uncontrolled storage have limited its application. Pre-sowing hydration invigoration accelerates and synchronizes the germination of deteriorated seeds. Ultra-fine bubble water (UFBW) contains reactive oxygen species, which can advance the pre-germinative metabolism of the seed. This study examined true shallot seed invigoration using UFBW. The experiment was conducted in a two-factor of randomized complete block design. The first factor was initial seed vigor based on the vigor index with six levels, i.e., 24, 15, 13, 12, 10, and 9%. The second factor was the invigoration treatment of UFBW (8 and 23 ppm dissolved oxygen concentrations) with two durations (24 and 48 h), 50 ppm GA3, and 3% KNO3 (24 h). UFBW with 23 ppm invigoration for 48 h improved seed vigor based on all vigor parameters. The GA3 50 ppm treatment is the most effective in increasing seed vigor. Keywords: dissolved oxygen; pre-sowing hydration; reactive oxygen species; seed viability; seed vigor
Pengembangan Metode Uji Tetrazolium dengan Sensor Infra red sebagai Uji Viabilitas pada Benih Kacang Tanah (Arachis hypogea L.) Widajati, Eny; Ansella, Yohanda; Dahlan, Ade Muhamad; Putro, Trisno Yuwono; Masitadewi, Ervin
Buletin Agrohorti Vol. 12 No. 2 (2024): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrob.v12i2.54759

Abstract

Pengujian viabilitas benih menggunakan uji tetrazolium merupakan salah satu metode penentuan viabilitas benih dengan mengevaluasi tingkat warna merah yang terbentuk pada benih. Warna merah terbentuk dari aktivitas enzim dehidrogenase pada siklus trichloroacetic acid. Penentuan tingkat warna merah pada benih ini dapat menggunakan alat sensor infra red. Penelitian ini bertujuan menguji alat sensor infra red untuk menentukan intensitas warna secara kuantitatif dalam uji tetrazolium serta menentukan korelasi alat sensor infra red dengan pengujian benih secara fisiologis. Percobaan disusun menggunakan rancangan kelompok lengkap teracak dengan faktor tunggal yaitu tingkat viabilitas benih berdasarkan daya berkecambah masing-masing adalah 78.9%, 70.7% dan 40.0 %. Hasil penelitian menunjukkan bahwa aplikasi sensor intensitas infra red untuk uji tetrazolium pada benih kacang tanah menghasilkan nilai koefisien korelasi (r) sebesar 0.62 antara nilai RGB merah bagian dalam kotiledon (Rd) dengan kecambah normal. Kata kunci: daya berkecambah, enzim dehydrogenase, kotiledon, mutu fisiologis benih, RGB
Fruit and Seeds Development and Seed Germination of Andaliman Pepper (Zanthoxylum acanthopodium DC.) Fahmi, Mochammad; Widajati, Eny; Palupi, Endah Retno; Rostiana, Otih
Journal of Tropical Crop Science Vol. 13 No. 01 (2026): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.13.01.75-93

Abstract

Andaliman is a species found in the Lake Toba region of North Sumatra, Indonesia. To determine its optimal harvest time and the length of its physiological maturity, it is necessary to observe fruit development in new locations outside its natural habitat. Andaliman seeds are known for their low germination rates and are classified as endospermic seeds. This study aims to analyze the stages of fruit and seed development, evaluate the effects of seed treatments, and identify the most effective germination test methods for andaliman seeds. Seeds were treated with a 50% H2SO4 solution for 15 min and with a 200 ppm GA3 + 100 ppm kinetin solution for 48 hr. The seeds were then tested using pleated paper and top paper media to assess germination response. Changes in fruit coloration indicated various stages of development: intense yellowish-green at 17 weeks after anthesis, deep yellowish-green at 20 weeks, deep red at 23 weeks, and dark greyish-yellowish-brown at 26 weeks. During development, some fruits experienced embryo abortion, resulting in many empty or wrinkled seeds. The seeds reached physiological maturity at 23 weeks after anthesis, as indicated by a deep red fruit color, increased seed dry weight, and enhanced germination and seed growth rates. The treatment with GA3 and kinetin yielded the highest germination rate of 20%, with the pleated paper method proving to be the most effective for germination testing.
Seeds morphology, anatomy, and quality of Musa balbisiana and Musa acuminata after storage Mertya Anugrah; Abdul Qadir; Eny Widajati; Sukartini
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 53 No. 2 (2025): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v53i2.59954

Abstract

The fluctuations in seed food reserves can trigger a cascade of physiological and biochemical events that ultimately manifest as discernible changes in seed morphology and anatomy.  The purpose of this research was to evaluate seeds morphology, anatomy, and quality of Musa balbisiana “Klutuk NTT” and Musa acuminata “Sumatrana” accessions and their storability. Morphological evaluation included seed size and the weight of 100 grains. Anatomical evaluations were carried out for seed structure. The seed quality of the Klutuk NTT banana accessions was observed after being stored for 5, 25, and 41 months, while the Sumatrana banana accessions were observed after being stored for 5, 23, and 41 months. The Klutuk NTT seeds are sized 4 mm to 5.8 mm, while Sumatrana seeds are sized 4 mm to 5 mm. The Klutuk NTT and Sumatrana seeds had a seed coat, endosperm, embryo, micropyle, inner and outer integuments, chalazal, and operculum. The embryonic structure of both Klutuk NTT and Sumatrana seeds exhibited an inverted T-shape. After storage, the two accessions showed significant differences in moisture content, germination rate, vigor index, and seedling growth performance. The Klutuk NTT accessions demonstrated a significant increase in moisture content, germination rate, vigor index, and seedling emergence uniformity, suggesting improved seed performance after storage. In contrast, the Sumatrana accessions showed a significant decline in all measured parameters, except for moisture content. These results suggest that the Klutuk NTT accession may possess a physiological seed dormancy mechanism. Keywords: Banana; germination; true seed; viability; vigor
Determination of maturity stage, after ripening period, and seed storability of upland rice seeds at various phosphorus fertilizer doses Irfan Firmansyah; Eny Widajati; Maryati Sari; Heni Purnamawati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 53 No. 3 (2025): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v53i3.67153

Abstract

The increasing demand for rice makes upland rice a promising alternative. However, one major challenge in upland rice cultivation is the availability of high-quality seeds. This study aimed to determine the physiological seed maturity, after-ripening period, storage longevity, and optimal fertilizer dose to produce high-quality IPB 10G Tanimar. The research used a split-plot randomized complete block design with two factors and three replications. The main plot was phosphate fertilizer doses (50, 100, and 150 kg ha-1 SP-36), and the sub-plot was seed maturity stages (112, 116, 120, 124, and 128 days after planting(DAP). The results showed that fertilizer dose had no significant effect on vegetative and generative growth. The application of 100 kg ha⁻¹ P fertilizer produced the best seed quality based on the initial vigor index. The best maturity stage was 124 DAS with a heat unit of 2,505 °Cd, as indicated by the parameters of initial vigor index, germination rate during storage, and storability. The shortest after-ripening period was also found at 124 DAS, with the minimum germination rate (>80%) achieved one week after storage (WAS). The best storage vigor at the end of the observation period (8 WAS) was at 124 DAS, with a germination rate of 79.67% and a vigor index of 21.89%.   Keywords: high-quality seed; heat unit; phosphate fertilizer; storage longevity; vigor index
Diseminasi Inovasi Varietas Unggul Padi IPB 12 S melalui Metode Demplot untuk Peningkatan Produksi Padi di Desa Wingkomulyo Eny Widajati; Muhammad Syukur; Ani Kurniawati; Setyo Pertiwi; Chusnul Arif; Silvan Timothy; Raihan Anwar Thaha
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 12 No. 2 (2026): Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.12.2.332-338

Abstract

The Bogor Agricultural University community service program aims to disseminate the superior rice variety IPB 12 S in Wingkomulyo Village, Ngombol District, Purworejo Regency. Wingkomulyo Village had 33 hectares rice field, with a good irrigation system. The implementation method includes coordination of program implementation, rice planting demonstration plots, observation of growth, development, and plant production. Planting was carried out with a spacing of 30 cm x 30 cm, 14-day-old seedlings. The fertilizer dose per hectare spread was 150 kg of urea, 400 kg of NPK 15-10-12 and 95 kg of NPK 16-16-16 and 1 liter of silica fertilizer. Plant growth from the vegetative phase to the flowering phase was very good, but the plants fell before harvest due to heavy rain and strong winds. The production components observed included the average number of productive tillers (13.0), the number of grains per panicle (141.7), and grain production (6.0 tons GKP ha-1), are still below the yield potential of the IPB 12S variety as stated in the Variety Release Decree. This activity successfully introduced the IPB 12 S variety and IPB Prima cultivation technology to farmers in Wingkomulyo Village.
Morphological and Phenological Study of Flowers and Fruits of Wild-Type Raspberries (Rubus spp.) Aminatun Nisa; Abdul Qadir; Eny Widajati; Muhammad Imam Surya
Journal of Tropical Crop Science Vol. 13 No. 02 (2026): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.13.02.445-456

Abstract

Wild-type raspberries are part of forest succession and could be cultivated owing to their benefits and economic value. The development of cultivated plants depends on the quality of the genetic material used for propagation. Phenological studies are the first step in assessing the quality of genetic material and in determining the overall developmental process. This research aims to study the development and morphology of flowers, fruits, and seeds. This research was conducted at Cibodas Botanical Garden from September 2022 to March 2023. The experiment used wild-type raspberry species as a factor in a randomized complete block design. The results showed differences in the developmental phases and morphology of flowers, fruits, and seeds, as well as in stigma receptivity, among R. fraxinifolius, R. rosifolius, and R. ellipticus. Flowers take 11–15 days from initiation to anthesis, and the receptivity of the stigmas of the three species reaches a peak one day after anthesis in the morning. The fruit is ripe at 39–46 days after anthesis with a different color.
Pola Topografi Pewarnaan Tetrazolium sebagai Tolok Ukur Viabilitas dan Vigor Benih Kedelai (Glycine max L.Merr.) untuk Pendugaan Pertumbuhan Tanaman di Lapangan , Dina; Eny Widajati; Baran Wirawan; Satriyas Ilyas
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 35 No. 2 (2007): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (59.639 KB) | DOI: 10.24831/jai.v35i2.1316

Abstract

Topographical tetrazolium (TZ) pattern to determine viability, vigor and field stand on soybean seeds cv. Burangrang was studied.  Laboratory test for standard germination, vigor index, speed of germination, accelerated aging, seedling growth rate and field performances were performed on five lots (A,B,C,D,E) of soybean seeds.  The viability and vigor categories of each pattern were determined by Root Mean Square (RMS), regression, and correlation analysis.  Ten topographical patterns were recognized. The laboratory test results and field performances were compared with topographical pattern. Combination of patterns 1,2,3,4 (embryonic axis and cotyledon completely and/or gradually stained) selected as viable category as it gave the least RMS value, the highest determination (R2) and correlation (r) coefficient with standard germination (RMS=6,46; R2=0,37; r=0,61). Combination of patterns 1,2,3 (embryonic axis and cotyledon completely stained) selected as vigor category as it gave the least RMS value, the highest R2and r with relative speed of germination (RMS=7,96; R2=0,44; r=0,66), vigor index (RMS=6,66; R2=0,36; r=0,6), field emergence (R2=0,79; r=0,89) and field stand (R2=0,65; r=0,80). The combination of patterns 1,2,3 is recommended for estimating field emergence and field stand.   Key words :  Tetrazolium, topographical pattern, viability, vigor, accelerated aging, field stand, soybean seed.
Pengaruh Kemasan, Kondisi Ruang Simpan dan Periode Simpan terhadap Viabilitas Benih Caisin Brassica chinensis L.) Esti Rahayu; Eny Widajati
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 35 No. 3 (2007): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (45.974 KB) | DOI: 10.24831/jai.v35i3.1330

Abstract

The objective of this trial was to determine the effect of the best  packaging material, storage room condition and storage period for the seed viability of caisin. The trial was conducted at the seed laboratory of the Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agriculture University in March- October 2005. The experiment was arranged in completely randomized design with three factors. The first factor was packaging materials (paper, polyethylene and aluminum foil); the second factor was storage room conditions (ambient room, Air Conditioned (AC) room and refrigerator); the third factor was storage periods (0, 3, 6, 9, 12 and 15 weeks). The result of this research showed that packaging materials gave significant effect on the dry weight of normal seedling. Storage periods gave very significant effect on germination percentage, dry weight of normal seedling, maximum growth capacity, and vigor index. The interaction between packaging materials, storage room conditions and storage periods gave very significant efflect on germination rate. Based on the dry weight of normal seedling, polyethylene and aluminum foil package gave the same result, on the other hand paper package gave the lowest dry weight of normal seedling than that aluminum foil package.  Based on the germination percentage, dry weight of normal seedling, maximum growth capacity, and vigor index the seed viability of caisin did not  decrease, even after 15 weeks of storage period.   Key words : Packaging materials, storage periods and seed viability
Co-Authors , Dina , Ramlafatma ,, Saipulloh . Giyanto Abdjad Asih Nawangsih Abdul Qadir Abdul Qadir Abdul Qadir Abdul Qadir Achmad Achmad Adhitya Vishnu Pradana Aminatun Nisa Anak Agung Keswari Krisnandika ANDI NADIA NURUL LATHIFA HATTA Anggraini, Dwi Vista Ani Kurniawati Annisa Nursoleha Ansella, Yohanda Arif, Mohamad ARYA WIDURA RITONGA Awang Maharijaya Bambang Sapta Purwoko Baran Wirawan Blair Moses Kamanga Chusnul Arif Cici Tresniawati Dahlan, Ade Muhamad Daniel Happy Putra Danu Kuncoro Devi Liana DEVI RUSMIN DEVI RUSMIN Devi Rusmin Diaguna, Ridwan Didy Sopandie Dirgahani Putri Dwi Gery Febriyan Dwi Guntoro Dyah - Manohara Dyah Manohara DYAH MANOHARA Edi Santosa Edi Santosa Edi Santosa Edy Suprianto Efendi, Darda ENDAH RETNO PALUPI Endah Retno Palupi Endah Retno Palupi ENDANG MURNIATI Esti Rahayu Esty Puri UTAMI Esty Putri Utami Esty Putri Utami Fahmi, Mochammad Faiza C. Suwarno Faiza C. Suwarno Faiza Chairani Suwarno Febriani, Lilis Yati Febriyan, Dwi Gery Fridayanti, Nelly GAGAD RESTU PRATIWI Gani Jawak Giyanto Giyanto Giyanto Giyanto Giyanto, dan Gundala, Belangie Tuahte Hadi, Putra K Hairani, Putri Mian Haura Fikriyah Hakimah Heni Purnamawati Herliyana Indahwardani Imroatus Sa’adah Indahwardani, Herliyana Irfan Firmansyah Iswari Saraswati Dewi Juliana Maia Kamanga, Blair Moses Kikin H Mutaqin Kresnamurti Kurniasih Krisnandika, A. A. Keswari Kusumastuti, Hamiddah Intan Kusumastuti, Shinta Nugraheni Lilis Yati Febriani M.R. SUHARTANTO M.R. SUHARTANTO, M.R. Maisaroh Tanjung MANOHARA, DYAH Manohara, Dyah - Maryati Sari Masitadewi, Ervin Mathius, dan Nurita Toruan Meity Suradji Sinaga Memen Surahman Mertya Anugrah Mia Kosmiatin Mohamad Arif Mohamad Arif Mohamad Rahmad Suhartanto Mubarak, Farahdina Muhamad Rezki Mauladi Muhamad Syukur Muhammad Afif Muhammad Imam Surya Muhammad Machmud MUHAMMAD MACHMUD Muhammad Machmud Muhammad Syukur Nadiya Iftiwata Rahmah Nelly Fridayanti Ni Made Armini Wiendi Nizaruddin . Norul Dewi Susanti Nur Syam Ismaniza A., Siti Nur Syam Ismaniza A. Nurita TORUAN-MATHIUS Nutrita Toruan Mathius Otih Rostiana Peni Handayani Permatasari, Okti Syah Isyani Pitri Ratna Asih Pramudita, Listya Putra K Hadi Putro, Trisno Yuwono Qadir , Abdul Qudus Sabha Adhinugraha R Pelealu, Rian Virvian Hidayat R. Palupi, Endah Rafi Fauzan Raga, Yuan Rahmah, Nadiya Iftiwa Raihan Anwar Thaha Riski Meliya Ningsih RUSMIN, DEVI RUSMIN, DEVI Sabar Pramono Sani, Mutiyara Sarah Desmia Muchtar Sarah Desmia Muchtar Satrias Ilyas SATRIYAS ILYAS Satriyas Ilyas SATRIYAS ILYAS SATRIYAS ILYAS Satriyas Ilyas Selly Salma Selly Salma Setyo Pertiwi Shinta Nugraheni Kusumastuti Silvan Timothy Siti Fadhilah, Siti Sobir Sobir Sri Suharyati Vityaningsih Sri Wilarso Budi SUHARTANTO, M.R. Suhartanto, Muhammad Rahmad Sukartini Supijatno Surya Kurnia Putra, Dicky Suwarno, Faiza C. Tanjung, Maisaroh Tantri Palupi Tati Budiarti Tefa, Anna Tri Astuti Trikoesoemaningtyas Trisno Yuwono Putro Wawan Hermawan Willy Bayuardi Suwarno Yuyuk Agung Lastiandika Yuyuk Agung Lastiandika ZULFIKAR DAMARALAM SAHID