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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Jurnal Silvikultur Tropika (JST) Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) Bioplante Bumi Lestari Agrikultura Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Keteknikan Pertanian Jurnal Penelitian Hutan Tanaman Agrovigor: Jurnal Agroekoteknologi Indonesian Journal of Agricultural Science Jurnal Hortikultura Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Pelita Perkebunan AGRIVITA, Journal of Agricultural Science Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Perlindungan Tanaman Indonesia BERITA BIOLOGI Jurnal Penelitian Karet JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Penelitian Kelapa Sawit AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Jurnal Hexagro Agro Bali: Agricultural Journal Abdi: Jurnal Pengabdian dan Pemberdayaan Masyarakat Jurnal Bioindustri Jurnal Penelitian Tanaman Industri (Littri) Jurnal Tanah dan Iklim Biofarm : Jurnal Ilmiah Pertanian Jurnal Risalah Kebijakan Pertanian dan Lingkungan Jurnal Penyuluhan Pertanian Proceedings Series on Physical & Formal Sciences Jurnal Pusat Inovasi Masyarakat PERTANIAN TROPIK Sewagati Policy Brief Pertanian, Kelautan, dan Biosains Tropika Journal of Bioindustry
Suryo Wiyono
Departemen Proteksi Tanaman, Fakultas Pertanian Insitut Pertanian Bogor Jl. Kamper, Kampus IPB Dramaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Journal : Jurnal AgroBiogen

 1 
Genetic Diversity Analysis Using Resistance Gene Analog-Based Markers to Support Morphological Characterization of Shallots Lina Herlina; Reflinur Reflinur; Kristianto Nugroho; Rerenstradika T. Terryana; Sobir Sobir; Awang Maharijaya; Suryo Wiyono
Jurnal AgroBiogen Vol 14, No 2 (2018): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v14n2.2018.p65-74

Abstract

Shallot (Allium cepa var. aggregatum) is one of the most important vegetable crops grown in Indonesia. The limited knowledge available on the genetic diversity and the threat of plant disease have been major problems to maintain high shallot production in Indonesia. Development of molecular markers linked to disease resistance is required for molecular breeding activity in this crop. This study aimed to assess the genetic diversity at conserved domain of resistance gene analog (RGA) in a set of 36 Indonesian shallot genotypes to complement morphological characterization. Twelve morphological and fifteen molecular markers traits were investigated in an attempt to characterize and to discriminate the Indonesian shallots genotypes. Characterization at orphological level indicated that phenotypic variance was highest for total bulb weight (TWB, cv = 99.39%) and the least for the plant height (PH, cv = 28.16%). The correlation analysis between traits showed that TWB and number of bulb (NB), TWB and bulb weight per plant (WB), NB and WB, and WB and PH were positively correlated. Molecular analysis revealed a total of 1,512 alleles with an average of 1.946 alleles per locus. The Polymorphism Information Content (PIC) values ranged from 0.253 to 0.676 and six out of 15 RGA markers were highly informative with PIC values ≥0.50. Based on cluster analysis, the 36 Indonesian shallot genotypes were clearly discriminated into six major groups. These results revealed that the RGA-based markers could support the morphological characterization in evaluating the genetic diversity of shallots. 
Resistance Responses of 35 Watermelon Genotypes to Three Isolates of Fusarium oxysporum f. sp. niveum Nazly Aswani; Suryo Wiyono; Sobir Sobir
Jurnal AgroBiogen Vol 17, No 2 (2021): DESEMBER
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v17n2.2021.p63-74

Abstract

Fusarium wilt caused by Fusarium oxysporum f. sp. niveum (Fon) is one of main diseases of watermelon. There have been very limited studies that tested watermelon genotypes to more than one isolates of Fon in Indonesia. This research aimed to determine the resistance of 35 watermelon genotypes to three Fon isolates taken from three different areas in Indonesia. Incubation period (IP) and disease index (DI) of the 35 watermelon genotypes were determined against three Fon isolates collected from Karawang (FK), Lampung (FL), and Purwakarta (FP). The experiment was arranged using a Completely Randomized Design with two replications. DI showed that six watermelon genotypes, i.e. New Hope, Sky Mountain, Southern Light, Super Sweet 66, Uranus, and Yellow Baby demonstrated moderate resistance to resistance phenotypes to all tested Fon isolates. IP and percentage of symptomatic plants (PSP) showed different responses among genotypes either to the same or to different isolates. Genotype New Orchid, for example, showed 57.14% symptomatic plants in less than 10 days after inoculation (DAI) when tested with FL isolate. Meanwhile, when tested with the same isolate, genotype New Dragon showed only 6.67% symptomatic plants in more than 23 DAI. The result of this study indicated that watermelon genotypes showing resistant to all tested isolates should be useful for breeding program to develop watermelon lines with broader resistance spectrum against Fon pathogen. The resistance genotypes selected should also demonstrate good agronomic performances and high yield to be considered as a new watermelon variety.
Genetic Diversity Analysis of 41 Chili Pepper Genotypes (Capsicum annuum L.) Based on SSR Markers Wartono Wartono; Suryo Wiyono; Muhamad Syukur; Giyanto Giyanto; Kristianto Nugroho; Puji Lestari
Jurnal AgroBiogen Vol 15, No 2 (2019): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v15n2.2019.p65-74

Abstract

Chili (Capsicum annuum L.) is an important horticultural crop which possesses high genetic diversity. Their genetic diversity needs to be assessed through molecular marker-based analysis to facilitate chili breeding scheme. The objective of the study was to analyze the genetic diversity of 41 chili genotypes using Simple Sequence Repeat (SSR) markers. Eleven SSR markers were used to amplify all genotypes through PCR technique, visualized on Polyacrylamide Gel Electrophoresis (PAGE). The data were analyzed using NTSYSpc version 2.1 and PowerMarker version 3.25 softwares. Results showed that the average of SSR alleles was 7.9 with a range of 4-11 alleles per marker. All SSR markers showed PIC value >0.5, indicating their suitable use for chili genetic studies. Cluster analysis of 41 genotypes generated three main groups according to coefficient similarity of 77.4%. The genetic clusters appeared as reflection of the genetic background of each genotype. In addition, the SSR markers used can identify potential parent as indicated by the genetic distance between genotypes. The highest genetic diversity (0.698) was demonstrated by combinations of Yuni × Ayesha, Yuni × Ayesha 2, and Yuni × Namira. The estimated genetic diversity produced by SSR markers in this study should be useful as guidance for chili breeders to select suitable parents for new cultivar development for high productivity and resistance to diseases.
Co-Authors . Haryanto . Rifka . Widodo . Widodo . Widodo Abdjad Asih Nawangsih ABDUL MUNIF Abdul Munif Adi Setyo Purnomo Agus Purwito Ahmad Fauzi Ridwan AHMAD JUNAEDI Ahmad Junaedy Ahmad Sutopo ALI NURMANSYAH Ali Wafa Aloysius Rusae Amrullah, Rizki Abi Anas Dinurrohman Susila Andika Septiana Suryaningsih Andriani, Desta Angelica Nabilla Ahdy Anisa Nurkasanah Anjar Kartika Antonius Suwanto ARI SUSILOWATI Arif Ravi Wibowo Aris Tri Wahyudi Asniah Asniah Astuti Kurnianingsih Atit Kanti Atit Kanti Awang Maharijaya Ayu Rahayu Baskoro Sugeng Wibowo Bayu Aji Krisandi Bonjok Istiaji Burhanudin Burhanudin Cahyani, Gesa Nur DADANG DADANG DAMAYANTI BUCHORI Daniel Happy Putra Darni Rambu D. Siala Dedi Fardiaz Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga DEWI SARTIAMI Dewi Sukma Dhany Nukhaizal Azkiya DWI ANDREAS SANTOSA Dwi Astuti DWI SUGIPRIHATINI Dyah Harianti Purnomo Efi Toding Tondok Eka Maulidiya, Sherly Eka Wijayanti Elis Nina Herliyana ENDANG MURNIATI Eryna Elfasari Rangkuti Evan P. Ramdan Fadillah, Arvin Muhammad Fakhrusy Zakariyya Fany Juliarti Panjaitan Fariyanti, Anna Fatah, Fadillah Abdul Fazad, M. Haikhal Febbiyanti, Tri Rapani Firman Hidayat Fitri Kemala Sandra Fitriansyah, Muhammad Ramdhani Giyanto Giyanto Giyanto Giyanto Giyanto Giyanto, Giyanto Halimah, Adedila Hanif, Andini HAPPY WIDIASTUTI Hari Priwiratama Harti, Heri Hendrastuti Hidayat, Sri Hendro Nurhadi Heny Setiyowati Heri Harti Hermanu Triwidodo Hidayah, Zahra Nurul Hidayat , Sri Hendrastuti I MADE ARTIKA I Made Sudiana I Made Sudiana I Nyoman Sumerta Idris Idris IKA DAMAYANTI Iman, Muhammad Ilham Nur Imaniasita, Vidya Indrapaksi, Tengku Ikhwan Alfajri Iskandar Z. Siregar Islah Hayati Ita Aprilia Ivone Oley Sumarauw Joni Hidayat, Joni Juang Gema Kartika Kartini Budiastuti Ketty Suketi Khairani Khairani Khairina, Winda Hidayatul Khamidi, Thamrin Khoirotul Afifah Kikin H Mutaqin Kristianto Nugroho Kristianto Nugroho Kudang Boro Seminar Kusuma Darma Kusuma Darma Kusumah, Yayi Munara Lestari, Mutiara Dwi Lilik Retnowati Lina Herlina Lina Herlina Listihani, Listihani M. Miftahudin Masrukhin Masrukhin Mei Lita Fitriani Meity S Sinaga Meity S. Sinaga Meity S. Sinaga MEITY S. SINAGA, MEITY S. Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Memen Surahman Mohamad Rahmad Suhartanto Mufidah, Iftin Farekana Muhammad Arief Munady Muhammad Lukman Hakim, Muhammad Lukman Muhammad Miftahudin Nanta, Wahyu Ridwan Napiudin Nazly Aswani Neo Endra Lelana nFN Khaerati Nika Ropiatningsuari Nur Alfi Saryanah Nur Khoiriyah Nurmala, Ayu Aulya Nurul Khumaida Octaviani, Wulan Nur Parida, Ida Phor Bho Ayuwati Pratama, Silvia Abdi Pratiwi, Astika Widhi Pratiwi, Tasya Resa Priyo Cahyono Puji Lestari Purnomo, Dyah Hariyanti Purwono Purwono, Purwono Puspitaningtyas, Khasanah Putri, Lystiana Dewi Putro, Herdayanto Sulistyo RAHAYU WIDYASTUTI Ramdan, Evan Purnama Reflinur Reflinur Reflinur Reflinur Remaja Sitepu Rerenstradika T. Terryana Riana Jumawati Rika Estria Gurusinga Rina Mardiana Rizki Abi Amrullah Roedhy Poerwanto Rohmah, Alya Awinatul Roza Yunita Rustam, Rustam Rustiani, Ummu S. Said Abdullah Saiful Akhyar Lubis Saputra, Bubun Afif Hidayat Aziz Sari Nurulita Sarwititi Sarwoprasodjo Satya Nugroho Sherly Eka Maulidiya Silviana Arsyad Sima Elpani Siregar, Amelia Nani Sitti Eha Faihah Sitti Fadhilah Slamet Susanto Sobir Sobir SRI FATMAWATI Sri Hartati Sri Hartati Sri Hartati SRI HENDRASTUTI HIDAYAT Sri Wening Stephanie Stephanie Sudarsono Sudirman Yahya Sudirman Yahya Sudirman Yahya Sudradjat Suhadi, Octen Sukmana, Andra Sahab Surya Rosa Putra, Surya Rosa Suryahadi Suryahadi Susanna Susanna SUSILOWATI1 SUSILOWATI1 Suwarno . Swastiko Priyambodo, Swastiko Tamrin Khamidi Tamrin Khamidi Tamrin Khamidi Tamrin Khamidi Teguh Iman Santoso Teguh Iman Santoso Titiek Siti Yuliani Toding Tondok, Efi Toga Pangihotan Napitupulu TRI ASMIRA DAMAYANTI Tri Rapani Febbiyanti Tri Rapani Febbiyanti, Tri Rapani Trikoesoemaningtyas Ummu S. Rustiani Ummu Salamah Rustiani Utami, Aditya Dyah UTUT WIDYASTUTI Vinsen Willi Wardhana Wahyu, Bonny Poernomo Wartono Wartono Wati, Cheppy Wawan Setiawan Weni Wilia Widhayanti, Nur Ari Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widya Sari Winarso D. Widodo Winda Ika Susanti Windah Meilanda Zulmi Yayu Siti Nurhasanah Yohana C Sulistyaningsih Yohana C Sulistyaningsih Yuli Fitriati Yuliawati YULIN LESTARI Yundari, Yundari Yuyun Andriyani