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Uji Aktivitas Antibakteri Glutathion terhadap Infeksi P. aeruginosa secara In Vitro Elissa, Intan Ayu; Mustikaningtyas, Dewi; Yuniastuti, Ari
Life Science Vol 9 No 2 (2020): November 2020
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v9i2.47161

Abstract

Pseudomonas aeruginosa is a gram negative bacterium that is pathogens opportunistic and the cause of nosocomial infections. P. aeruginosa is resistant to antibiotics that are often given. The aim of this study is to prove the presence of antibacterial activity of isolated glutathione from S. cerevisiae on P. aeruginosa infection. The design of this study is a completely randomized design. The variation concentration used was 100%, 75% and 50%. The parameters observed were inhibition zone diameter, turbidity level and the presence or absence of growth in MHA media. The results showed that the diameter of the largest inhibition zone was found at a concentration of 100% which was 7.88 mm and included a moderate inhibition criteria. The clearest solution after 24 hours incubation is a solution with an isolated glutathione concentration of S. cerevisiae 100%. While when inoculated into MHA media, all concentrations still produce P. aeruginosa growth. It means that the isolated glutathione from S. cerevisiae is bacteriostatic. Based on the results of these studies, it can be concluded that the isolation of glutathione from S. cerevisiae has antibacterial activity against P. aeruginosa so that can be used as an alternative treatment for P. aeruginosa infection. Pseudomonas aeruginosa merupakan bakteri gram negatif yang bersifat patogen oportunistik dan penyebab infeksi nosokomial. P. aeruginosa resisten terhadap antibiotik yang sering diberikan. penelitian ini bertujuan untuk membuktikan adanya aktivitas antibakteri dari glutathion hasil isolasi dari S. cerevisiae terhadap infeksi P. aeruginosa. Rancangan penelitian ini adalah rancangan acak lengkap. Variasi konsentrasi yang digunakan adalah 100%, 75% dan 50%. Parameter yang diamati adalah diameter zona hambat, tingkat kekeruhan dan ada tidaknya pertumbuhan pada media MHA. Hasil penelitian menunjukan bahwa diameter zona hambat paling luas terdapat pada konsentrasi 100% yaitu 7,88 mm dan termasuk kriteria penghambatan sedang. Larutan yang jernih setelah inkubasi 24 jam adalah larutan dengan konsentrasi glutathion hasil isolasi dari S. cerevisiae 100%. Sedangkan ketika diinokulasikan ke media MHA, semua konsentrasi masih menghasilkan pertumbuhan P. aeruginosa. Hal tersebut berarti glutathion hasil isolasi dari S. cerevisiae bersifat bakteriostatik. Berdasarkan hasil penelitian tersebut, dapat disimpulkan bahwa glutathion hasil isolasi dari S. cerevisiae memiliki aktivitas antibakteri terhadap P. aeruginosa. Sehingga, glutathion hasil isolasi dari S.cerevisiae dapat digunakan sebagai alternatif pengobatan terhadap infeksi P. aeruginosa.
PENGEMBANGAN OLAHAN HASIL TERNAK MELALUI DIVERSIFIKASI PRODUK BERBAHAN DASAR SUSU DI KELURAHAN CEPOKO GUNUNGPATI SEMARANG Yuniastuti, Ari; Setiati, Ning; Susanti, R
Rekayasa : Jurnal Penerapan Teknologi dan Pembelajaran Vol 15, No 1 (2017): Juli 2017
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/rekayasa.v15i1.12561

Abstract

Kelurahan Cepoko is one of 16 urban villages in Gunungpati sub-district. The source of income of Cepoko village residents is in the field of livestock, as much as 77 residents of Cepoko urban village who rely their life in the field of farms, both chicken farms, dairy cows and meat cows. Dairy cattle breeding activities do not provide sufficient income, because the selling price of milk purchased by cooperatives is too cheap, so it can not cover operational costs. Therefore, one of the citizens of SME RW 09 kelurahan Cepoko that is Ibu Ismiyati took the initiative to make processed foods made from milk. Mrs. Ismiyati as a pioneer of milk sticks production business that is personal with employ 5% of women PKK group. Dairy products that have been done just limited to milk stick. Though dairy products have the potential to be developed in order to increase farmers’ income. One of the obstacles to the development of dairy products is the lack of mastery of information on production and marketing techniques by mothers, the limited knowledge and skills of mothers in the processing of milk into products worth selling, causing dairy products are less varied and not devel- oped. The methods undertaken to improve knowledge on milk-based food production techniques are socialization, training and monitoring. PKK ladies are very enthusiastic to follow the milk process- ing into a variety of food products that are proclaimed with the presence of mothers despite the rain, they still spend time attending training activities. PKK ladies become aware of the diversification of products based , and skilled in processing milk into various foods as well as skilled in the produc- tion manejemn such as kenmas and marketing processed products kamanan Participants want more follow-up is more real in terms of assistance and continuous monitoring and marketing of dairy- based food products broadly and poured in MoU between FMIPA and Gunungpati District
The Effectiveness of A Studdent Facilitator and Explaining Learning Model With A Roundhouse Diagram on Studdent Activity and Achievement in Virus Material Wahyuningtyas, Arini Tri; Yuniastuti, Ari; Utami, Nur Rahayu
Journal of Biology Education Vol 11 No 1 (2022): April 2022
Publisher : FMIPA UNNES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbe.v11i1.50266

Abstract

Results of the observation shows that students are still passive during the learning process in which influence their learning achievement. A learning model of Student Facilitator and Explaining (SFaE) is effective to make the students explain the materials to their friends also to train students in expressing their ideas bravely. This study combines SFaE with Roundhouse diagram that makes students easier to understand and explain the materials of learning. The research was conducted for the tenth grade students of SMA N 1 Jekulo. A purposive sampling was used as the sampling technique, and two classes were used as the sample. The T-test shows that the t-value is more than t-table for 2.71 > 1.67 with which means H0 is rejected. This condition means that the average results of the experimental class is higher than the control class. The results of analysis on students’activity in the experimantal class is around 91.76% students are in the category of active until very active, and 8.33% are in the category of medium. In the control class, around 80.56% students are in the category of active until very active, and 19.44% are in the category of medium. In conclusion, the use of learning model Student Facilitator and Explaining (SFaE) with a Roundhouse diagram is effective to improve students’activity and achievement in the learning material of Virus.
Empowerment Of Pospaud Cades In Management Of Posture "Melati" Cepoko Gunungpati Semarang Ari Yuniastuti
Jurnal Berkarya Pengabdian Masyarakat Vol 1 No 2 (2019): Berkarya Pengabdian Kepada Masyarakat
Publisher : Pusat Kajian Pendidikan Olahraga, Kesehatan dan Rekreasi Jurusan Pendidikan Olahraga Fakultas Ilmu Keolahragaan Uiveristas Negeri Padang Jl. Prof. Hamka, Air Tawar Padang 25131 Telp. 0751-7059901

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/jba.v1i2.20

Abstract

PosPAUD is a form of early childhood education services (PAUD) whose implementation is integrated with the services of Bina Keluarga Balita (BKB) and Posyandu. PosPAUD "Melati" is one of the PAUD Posts in Gunungpati Subdistrict. Based on PosPAUD data "Melati" Getas Cepoko village, the number of children active in PosPAUD aged 0-6 years in 2017 reached 25 children, while in 2018 active PAUD students were 18 people, there was a decrease in the number of community participation in PAUD activities. Likewise in the Posyandu activities which are held routinely on Saturdays on the first week of each month, the longer the number of people who come there is less The main problems faced by this pospaud include the limited number of cadres, the low knowledge and skills of cadres about nutrition and health and nutritional supplementary feeding (PMT) based on local food ingredients, the limited facilities available, the lack of knowledge about administrative order and active-creative learning. Community service methods are applied through pospaud cadre training, and assistance in the implementation of pospaud. Pospaud cadre empowerment activities carried out for 4 months, contributed 1). Increased cadre knowledge in managing PosPAUD; 2) Posyandu cadre skills in providing counseling and counseling about nutrition and health to children participating in PosPAUD and mothers of children under five; 3) Increased knowledge of PosPAUD cadres about nutritional supplementary feeding (PMT) based on local food ingredients; 4) PosPAUD cadre skills in making PMT (Supplementary Feeding) increased, and cadres could innovate types of PMT based on local food ingredients; 5) PosPAUD room is more comfortable and attractive; 6) Establishment of Family Medicine Park (TOGA). The results of the posyandu cadre empowerment activities showed an average initial knowledge of posyandu cadres on various aspects of being cured by 40.45%; the average posyandu cadre final knowledge on various aspects of being educated and trained is 95.25%; thus increasing the final evaluation results by 54.80% (classified in the medium category). The equipment assistance provided is able to support the smooth operation of the Melati pospaud activities. Keyword : pospaud, nutrition, supplmenetray feeding
Metagenomic analysis of intestinal microbiota in geese from different farming systems in Gunungpati, Semarang R Susanti; Ari Yuniastuti; Fitri Arum Sasi; Muchamad Dafip
Indonesian Journal of Biotechnology Vol 25, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.53936

Abstract

The diversity of intestinal bacteria in geese correlates with environmental conditions, rearing methods, and consumed feeds. The intestinal bacteria composition is useful for the absorption of nutrition, improving the metabolism, and may be related to the immune system. This study was conducted to examine the intestinal bacteria composition and the diversity of maintained goose in aviaries and barns. This research was an observational exploratory. Five geese were taken purposively from local breeders in Gunungpati District, Semarang City. A total of 5 g of intestinal contents from each sample was used for microbial genome isolation. Then, the genome was amplified to collect 16S rRNA gene region V3-V4. The amplicons were then sequenced using the next generation sequencing (NGS) method (Illumina high-throughput sequencing; paired-end reads) and analyzed using QIIME2 to identify bacterial species. In addition, GC-MS was performed to identify and measure fatty acid contents in the intestinal. The results showed that both rearing and caged goose contained nine phyla of intestinal bacteria. The number of intestinal bacteria of barn geese (SU) reached 32,748 Operational Taxonomy Units (OTU); higher than aviary geese (SK), which was 11,646 OTU. The intestinal bacteria community in barn geese was approved by Phylum TM7 (Saccharibacteria candidate) (53.18%), followed by Firmicutes (32.51%) and Bacteriodetes (5.42%). Whereas on SK Firmicutes was compiled 49.3 4% of total OTU, TM7 (S. candidate) up to 21.17%, and Actinobacteria up to 15.99 %. The abundance of TM7 may contribute to high 9,12-octadecadienoic acid production, while Firmicutes was related to the high production of oleic acid. Based on these data, the reared geese had a more abundant diversity of bacteria than the caged one.
IndonesiaDeteksi Escherichia coli O157 pada air minum di Kelurahan Sekaran Gunungpati Semarang Devi Dwi Jayanti; R Susanti; Ari Yuniastuti; I Wayan Suardana
Jurnal Biologi Udayana Vol 24 No 2 (2020): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2020.v24.i02.p01

Abstract

Penelitian bertujuan untuk mendeteksi adanya bakteri Escherichia coli O157 pada air minum kemasan, air minum isi ulang, dan air sumur di Kelurahan Sekaran Gunungpati Semarang. Sampel yang diambil sebanyak 20 sampel yang terdiri atas 4 merk air minum kemasan, 8 sampel air minum isi ulang, dan 8 sampel air sumur. Penelitian diawali dengan tahap isolasi E.coli pada medium Eosin Methylen Blue Agar (EMBA), yang dilanjutkan ke medium Sorbitol MacConkey Agar (SMAC) untuk identifikasi E.coli O157 dilanjutkan uji lateks aglutinasi (OXOID) dan diakhiri dengan uji konfirmasi gen rfbE menggunakan teknik Polymerase Chain Reaction (PCR). Hasil penelitian menunjukkan 8 sampel yang positif E.coli pada medium SMAC menunjukkan positif E.coli O157 (colorless). Uji lateks aglutinasi juga menunjukkan 8 sampel positif E.coli O157 seperti kontrol ATCC 43894. E.coli ATCC 43894 dan 8 sampel E.coli dari berbagai air minum di Kelurahan Sekaran Gunungpati Semarang menunjukkan positif E.coli O157.
Correlation of Heavy Metals Cd, Cr, Cu, Hg, Pb, and Zn with Intestinal Bacteria in Anas platyrhynchos L. Duck R Susanti; Ari Yuniastuti; Muchamad Dafip; Fidia Fibriana
HAYATI Journal of Biosciences Vol. 29 No. 4 (2022): July 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.4.459-467

Abstract

Intestinal bacterial species play an essential role in the duck body metabolism and productivity. They are affected by various factors, such as heavy metal contamination. This research aims to study metal emission levels and their correlation with the composition of intestinal bacteria. A total of twenty-five duck samples (Anas platyrhynchos L.) were obtained from Semarang, Temanggung, Magelang, Pati, and Salatiga. Five g of intestinal substances were employed in the isolation 16S rRNA gene V3-V4 region. Data sequence in fastqc format was then analyzed using QIIME. Also, the heavy metals in water, feed, and meat samples were analyzed using inductively coupled plasm-optical emission spectroscopy (ICP-OES). The highest heavy metal contaminant was Hg, with a total concentration reaching 5.55±1.79 ppm of input and 4.42±0.80 ppm in meat. The bacterial composition comprises the four highest phyla, sequentially including Firmicutes, Actinobacteria, Proteobacteria, and Bacteriodetes. The relationship between heavy metals and intestinal bacteria shows a significant value with a coefficient of >0.90. Therefore, there was a correlation between heavy metals and intestinal bacteria composition, suggesting that bacteria are likely to protect ducks from the adverse effects of heavy metals in water and feed.
PENGARUH PEMBERIAN MADU KELENGKENG TERHADAP AKTIVITAS ENZIM SUPEROXIDE DISMUTASE DAN KATALASE PADA TIKUS YANG DIINDUKSI Pb Asetat Ari Yuniastuti; Kamilatussainah Kamilatussainah; Fitri Arum Sasi
Prosiding SNST Fakultas Teknik Vol 1, No 1 (2015): PROSIDING SEMINAR NASIONAL SAINS DAN TEKNOLOGI 6 2015
Publisher : Prosiding SNST Fakultas Teknik

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penelitian ini bertujuan untuk mengetahui  aktivitas enzim antioksidan superoksida dismutase (SOD) dan katalase dalam plasma tikus wistar yang diiunduksi Pb asetat dan suplementasi madu kelengkeng. Penelitian eksperimental laboratorik, dengan rancangan The Post Test-Only Group Designdilakukan pada bulan Maret 2015 menggunakan hewan coba tikus putih wistar umur 2-3 bulan dengan berat badan 150-250. Sebanyak 20 ekor tikus diadaptasi dan diberi pakan standar selama 2 minggu. Selanjutnya dibagi secara acak menjadi 4 kelompok, yaitu : kelompok kontrol hanya diberi pakan standar,  K(-): kelompok tikus yang diberi perlakuan Pb asetat 10 mg/kgBB, P1 : kelompok tikus yang disuplementasi madu kelengkeng 0,9 ml/200 grBB dan Pb asetat 10 mg/kgBB, P2: kelompok tikus yangdisuplementasi madu kelengkeng 1,8 ml/200 grBB dan Pb asetat 10 mg/kgBB dilakukan selama 14 hari. Setelah akhir perlakuan tikus diambil darahnya melalui plexus retroorbitalis untuk diukur kadar SOD dan CAT.  Hasil pemeriksaan dianalisis dengan uji Chi Square dengan batas signifikansi = 0,05.Hasil penelitian menunjukkan bahwa pada tikus kelompok kontrol kadar SOD 141,50μ/ml dan CAT 0,8μ/ml; K(-) kadar SOD 77,71μ/ml dan CAT 0,12μ/ml; P1 kadar SOD 289,82 μ/ml dan CAT 1,21. Sedangkan pada tikus P2,kadar SOD 391,59 dan kadar CAT 3,62. Secara statistik terdapat perbedaan signifikan antar kelompok. Kata kunci: antioksidan, katalase, madu kelengkeng, superoksid dismutase
PERAN PANGAN FUNGSIONAL DALAM MENINGKATKAN DERAJAT KESEHATAN Ari Yuniastuti
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2014: PROSIDING SEMINAR NASIONAL HASIL - HASIL PENELITIAN & PENGABDIAN
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (144.095 KB)

Abstract

Pangan fungsional adalah pangan yang karena kandungan komponen aktifnya dapatmemberikan manfaat bagi kesehatan, di luar manfaat yang diberikan oleh zat-zat gizi yangterkandung di dalamnya. Tumbuh-tumbuhan merupakan salah satu bahan alami tertua yangdiketahui sebagai sumber pangan fungsional. Selain tumbuh-tumbuhan, beberapa bakteri jugamerupakan sumber pangan fungsional. Kajian tentang pangan fungsional dalam perspektifkesehatan telah banyak dilaporkan. Hasil penelitian tentang acemannan dan glucomanan lidahbuaya dan bakteri lactobacillus sebagai pangan fungsional akan dikaji dalam tulisan ini.Senyawa bioaktif acemannan dan glucomanan pada tanaman lidah buaya (Aloe vera) berperanpada keseimbangan sistem imun tubuh dan kadar lemak darah. Sedangkan bakteri lactobacillusberperan dalam menjaga keseimbangan mikroflora usus, membantu menurunkan kadar lipiddarah. Semoga kajian tentang peran senyawa bioaktif acemannan dan glucomannan serta bakterilactobacillus dapat menambah khasanah peran pangan fungsional dalam meningkatkan derajatkesehatan.
Association of rs10830963 MTNR1B and rs841853 SLC2A1 Polymorphism with Obesity on Type 2 Diabetes Patients: An Overview of Melatonin Receptor and Transporter Yanuarita Tursinawati; Arum Kartikadewi; Ari Yuniastuti; R Susanti
The Indonesian Biomedical Journal Vol 13, No 2 (2021)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v13i2.1488

Abstract

BACKGROUND: One of the hormones that plays a role in glucose metabolism of Type 2 Diabetes Mellitus (T2DM) is melatonin. Its genetic variation is believed to play a significant role in the pathophysiology of obese and non-obese T2DM. The role of MTNR1B (melatonin receptor coding gene) and SLC2A1 (Glucose transporter 1/GLUT 1 transporter coding gene) on the risk of obese and non-obese T2DM patients is controversial. This study aims to analyze the association between the rs10830963 MTNR1B and rs841853 SLC2A1 polymorphism to the risk of Javanese obese T2DM.METHODS: This was a cross-sectional study that involved 107 Javanese T2DM patients from primary heath care in Semarang. Furthermore, obese T2DM was defined by a body mass index (BMI) more than 25 kg/m2. The genetic variations examined were rs10830963 MTNR1B and rs841853 SLC2A1 polymorphism by PCR-RFLP methods. Blood biochemistry parameters were also examined. Allele and genotype frequencies of rs10830963 MTNR1B and rs841853 SLC2A1 polymorphisms were analyzed using the x2 test with p≤0.05 and 95% CI.RESULTS: There was a significant association between rs10830963 MTNR1B polymorphisms in obese and nonobese T2DM (p=0.044) and the CG genotype increased the risk of obese T2DM. Furthermore, the allele and genotype frequency of rs841853 SLC2A1 polymorphism in both group had no significant difference, with a p=0.756 and p=0.802, respectively. There was also no significant difference in the biochemical parameters' in both groups of the two genetic variants studied.CONCLUSION: The rs10830963 MTNR1B polymorphism is associated with the risk of obesity in Javanese T2DM patients but not for the rs841853 SLC2A1 polymorphism.KEYWORDS: polymorphism, MTNR1B, SLC2A1, obese, diabetes mellitus
Co-Authors Abdurrahman Ali, Nur Aini Abudu, Komaria Ode AD Safarina Adevia Maulidya Chikmah, Adevia Maulidya Adhisti Lutfia Hanan Adhisti Lutfia Hanan Adiba, Daniar Arof Adiba, Daniar Arof Adystia, Yusdike Aennie Widiastuti, Vinka Afniyati, Nur Afniyati, Nur Agnes Dwi Safarina Agnes Dwi Safarina Aidah, Nur Amalia, Devi Andreas Priyono Budi Prasetyo Anggit Kartikasari Anggraeni Noviasari, Nina Ani Rusilowati Anita Dyah Listyarini Arif Kurniadi Ariyanto, Andhika Arum Kartikadewi Arum Kartikadewi Arum Kartikadewi Asih Kuswardinah Asyumdah, Asyumdah Azkiyah, Nimas Catur Budi Laksono Budi, Dini Wurnaning Budu - Candrawan, Roni Prasetyo Chatila Maharani Darmayanti, Mey Linda Devi Dwi Jayanti Dewi Mustikaningtyas Dina Nur Anggraini Ningrum Dinda Fuji Islamiyanti Dyah Rini Indriyanti Eko Farida Elissa, Intan Ayu Ell Khaq, Arrina Ely Rudyatmi, Ely Enni Suwarsi Rahayu Eunike Raffy Rustiana Evi Widowati F. Putut Martin HB Farida, Eko Faridho, Fikrifar Rizki Fathnin, Fildza Huwaina Fatiah, Nirmala Al Faza Khilwan Amna, Faza Khilwan Feri Dwi Agustina Feri Dwi Agustina Fidia Fibriana Fitri Arum Sasi Fitri Arum Sasi, Fitri Arum Halimah, Khori Handayani, Kasmini Hidayah, Arifatul Hidayah, Salis Nur I Wayan Suardana Ibnul Mubarok Ibnul Mubarok Indah Hartati Indriani, Betty Innayah, Nurul Intan Zainafree, Intan Irawan Yusuf Irawan Yusuf Irwan Irwan Isnaeni, Lili Astuti Isnaeni, Lili Astuti Isti Hidayah Isti Mulyawati Istianah Istianah Istiqomah Dwi Andari, Istiqomah Dwi Istiqomah, Annisa Aulia Jiwanjaya, Yoga Juwita, Fitra Kamal Agus Efendi Kamala Nuriyah Kamilatussainah Kamilatussainah Kamisih, Kamisih Karomika, Ati Kasimi, Oktia Woro Khikmatul Maula, Lia Kirana Rannoe Tirta Kirana Rannoe Tirta Krispinus Kedati Pukan Lala, Salsabila Kinaya Pranindita Laraswati, Vivi Anggraeni lathifah, Ummu Lindasari, Pamela Lisdiana Lisdiana Lisdiana Lucita, Fifty K. Lyndasari, Pamela Mahalul Azam Martien Herna Susanti Mayang Siwi Dewayanti, Mayang Siwi Merzistya, Aufiena Nur Ayu Mohamad Endy Y Yulianto Muchamad Dafip Muchamad Dafip Muh Nasrum Massi Muh. Nasrum Massi Muslimah Muslimah Nabila Azzahra Luthfiah Nabila Azzahra Luthfiah Nadhifah, Shiliya Lana Nadhifah, Shiliya Lana Naufalia Riz Asmara, Qeyza Nbaia, Sara M Mohamed Negarani, Eryca Nur Bela Negarani, Eryca Nur Bela Niken Subekti Ning Setiati Nirmala Al Fatiah Nirmala Al Fatiah Noor Aini Habibah Noor Hidayah Noor Hidayah Nugrahanigsih WH Nugrahaningsih Nugrahaningsih Nugrahaningsih W H Nugrahaningsih WH Nugrahaningsih WH Nugrahaningsih WH, Nugrahaningsih Nugrahaningsih, WH Nur Kusuma Dewi Nur Rahayu Utami Nurussalma, Syafina Octarini, Dewi Oktia Woro Oktia Woro Kasmini H Oktia Woro Kasmini Handayani Parmin - Pramesti Dewi Pramusinta, Linda Prasetyowati, Tri Ari Priyantini Widiyaningrum Puspita Diana Arumawati Putri, Siti Rafika R Susanti R Susanti R Susanti R Susanti R Susanti R Susanti R Susanti R, Rahmatika Saputri Rahardjo, Bambang Budi Rahayu, Rr. Sri Ratna Rahmatih, Aisa Nikmah Rahmatih, Aisa Nikmah Ramasamy, Sujatha Ratna Rahayu, Sri Ratna Rahayu, Sri Resti Fitriati Retno Sri Iswari Retno Sri Iswari Retnoningsih, Heriani Rochly, Revinta Nur Aulia Rofika, Ana Rohmah, Amalia Nor Rr. Sri Ratna Rahayu Sa’adah, Siti Rofiatus Saiful Ridlo Satriani Satriani Sekar Arum, Vriska Roro Setyoko Setyoko Sholeha, Siti Sholikah, Anik Sholikah Shovrotul Khoiriyah, Ely Sigit Saptono Siti Alimah Siti Alimah Siti Baitul Mukarromah Siti Isnaeni SITI MAHMUDAH Siti, Putri Rafika Sowwam, Muhammad Sri Mulyani Endang Susilowati, Sri Mulyani Endang Sri Ratna Rahayu, Rr Sri Sukaesih Sugianto Sulasfiana Alfi Raida, Sulasfiana Alfi Sulistiani, Duwi Sulistyawati, Anita Sumarti Sumarti Suryo Pratikwo Suyadi Suyadi Swasty, Swasty Syarifah, Aidah Nur Talitha Widiatningrum Totok Sumaryanto Florentinus, Totok Sumaryanto Tyas Agung Pribadi Umam, Syaiful Ishlahul Utami, Hanna Dewi Utami, Hanna Dewi Vilda Ana Veria Setyawati Vilda Ana Veria Setyawati Vilda Ana Veria Setyawati Vilda Ana Veria Setyawati Virtanti, Uji Wahyuningtyas, Arini Tri Wan Jasimah Bt Wan Mohamed Radzi, Che Widya Hary Cahyati Wiwi Isnaeni Woro K H, Oktia Wulan Christijanti Yanti, Erny Yuni Yanuarita Tursinawati Yanuarita Tursinawati Yanuarita Tursinawati Yanuarita Tursinawati Yuni Wijayanti yuniarti, christina ary Yusdike Adystia Zaenafree, Intan Zunikhah Zunikhah Zunikhah Zunikhah