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Journal : BIOVALENTIA: Biological Research Journal

CONDITION OPTIMIZATION PRODUCTION XYLANASE THERMOSTABLE BY Bacillus licheniformis TS10 USING SUBSTRATE OIL PALM EMPTY FRUIT BUNCHES Safitri, Ayu; Muharni, -; Kusumawati, Heni Yohandini
BIOVALENTIA: Biological Research Journal Vol. 3 No. 1 (2017)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (133.065 KB) | DOI: 10.24233/BIOV.3.1.2017.51

Abstract

Optimization conditions for thermostable xylanase production of Bacillus licheniformis TS10 by using substrate oil palm empty fruit bunches  has been conducted from November 2015 to January 2016 in the Laboratory of Genetics and Biotechnology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Indralaya. The aims of this study was to determine the potential of oil palm empty fruit bunches ( EFB ) as a substrate for the production of thermostable xylanase by Bacillus licheniformis TS10 and the optimum conditions of temperature, pH and substrate concentration in the fermentation process. The research method is to make the growth curve and the curve of Bacillus licheniformis TS10 enzyme production on EFB substrate. The number of bacterial cells was determined by using a UV-VIS spectrophotometer and total plate count (TPC) through a standard curve. Optimization performed at various pH (5, 6, 7, 8, 9), temperature (50 °C, 60 °C, 70 °C, 80 °C) and substrate (1%, 2%, 3%, 4%) , Test activity at each pH, temperature and substrate using methods DNS by measuring enzyme activity based on the reducing sugar released by the substrate by using dinitrosalicylic acid (DNS). Based on the results of this research showed that oil palm empty fruit bunches (EFB ) has potential as a substrate for the production of thermostable xylanase by Bacillus licheniformis TS10, thermostable xylanase production of Bacillus licheniformis TS10 on the substrate EFB has an optimum condition at pH 6, 80 °C and the substrate concentration of 4%.
THE EFFECT OF APPLICATED ASH SAWDUST ON SWAMP LAND (INDRALAYA) FOR VEGETATIVE GROWTH OF RED PEPPER (Capsicum annum L.) VAR. CEMETI Pratama, Rahmat; Adriyansah, Fikri; Safitri, Ayu; M.S, Rahman Ismail
BIOVALENTIA: Biological Research Journal Vol. 3 No. 2 (2017)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.856 KB) | DOI: 10.24233/BIOV.3.2.2017.81

Abstract

The research " The Effect of Applicated Ash Sawdust on Swamp Land (Indralaya) For Vegetative Growth of Red Pepper (Capsicum annum L.) var. Cemeti ’’ has done, from  September to November 2017. It was conducted at green house and Pest Diseases Plant Laboratory, Plant Protection Department, Agriculture Faculty, University of Sriwijaya. This research was aimed to know the effect of applicated ash sawdust on vegetative growth of red pepper. The Completely Randomized Design with treatments are : 10, 20, and 30 g/ kg marshland and without ash sawdust (control), with 6 replicates each treatments. The result showed that applicated ash sawdust on measure 10 g/ kg soil significantly on number of growth 53.28 cm and number of branch 9.2, but not differ on dry weight of red pepper.Keywords :  Swamp land, ash sawdust, branch, red pepper 
EXTRACTION AND PURITY DNA OF Culex spp MOSQUITO IN KEMELAK VILLAGE, BINDUNG LANGIT, OGAN KOMERING ULU Nuriessa Aputri, Farah; Hanum, Laila; Safitri, Ayu; Windusari, Yuanita
BIOVALENTIA: Biological Research Journal Vol. 7 No. 2 (2021)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (500.938 KB) | DOI: 10.24233/biov.7.2.2021.217

Abstract

Culex spp are mosquito vectors that have a very wide distribution capability and are carriers of pathogens that can interfere with human and animal health. The wide distribution makes Culex spp a dangerous threat. DNA extraction is one of the important steps in obtaining genetic information and genetic analysis. Good quality DNA is used for activities such as the use of molecular markers, genome library creation, and sequencing. This study aims to determine the quality, concentration and purity of Culex spp mosquito DNA in Kemelak Bindung Langit Village, OKU Regency. It is hoped that the sample can be used for further research analysis on Mitochondria D-Loop Sequences in Culex spp mosquitoes. Quantitative measurement of DNA in the form of concentration and purity of DNA using Nanodrop Thermo cycle while qualitative DNA using electrophoresis technique. The results of the isolation of the mosquito genome DNA, obtained clear DNA bands without any degradation (smear) and the concentration results for the four samples ranged from 10-100 ng/µL and the DNA purity was good, ranging from 1.8 to 2.00.
Co-Authors . Hasmunir, . Afriyani, Asia Agustiansyah, Lucky Dita Ahmad Guntur Alfianto Al fathih Niska Adilah Amsal Amri Ananda Rizky Fadillah Andriyani Puji Lestari Arianto Arianto Arista Anindya Ramadhani Arya Sheva Satria Dewantara Asrori, Moh Syafaat Carolyn Octavia Cornelyanto Dea Fadilla Chairunisa Dea Puspitasari Desi Riawanti Desty Endrawati Subroto Dewi Fatmarani Surianto Dewi Safitri Dina Trianggaluh Fauziah Dyan Wigati Edy, Marwan Ramdhany Eka Damayanti, Fauziah Eka Prasetya Budi Mulyawan Elmania, Putri Fajar Hidayanto, Fajar Fathia Hanifa Syahida Fatima Az-zahra Wairooy Fatimah Aminati Fikri Adriyansah, Fikri Firdaus Firdaus Fitri Rosdiana Fratiwi, Ineke Halla, Maulana Ahsan Hanaa Azkia Hargianti, Mita Hari Hariadi, Hari Harvianti, Yuniar Hendrokumoro Hendrokumoro, Hendrokumoro Heni Yohandini Hikam, Moch Hikmah Puspasari Ibrahim, Irianto Ihsan Firmansyah Iqbar, Muhammad Iskandar, Yandra Jumadi Mabe Parenreng Khoirunnisa Khoirunnisa Koyyimahtul Munawaroh Krisnandi, Herry Kusdinar, Yopi Laila Hanum Lailatul Zannah Lamazi, Lamazi Levy Olivia Nur Lubis, M. Ilyas Lubis, Nur Ainun Lubis, Ulfa Annisa M. Farhan Hidayatullah M. Fariz Fadillah Mardianto M. Mahbubi Maesaroh Maesaroh Martinus, Ali Mega, Zakria Ferisya Meilana Afif Mahmudi Mia Audina, Mia Mindo Mursalina Jen Mirza Desfandi Moch Alif Haqi Mochammad Reihan Rabbani Moh Rizal Riandi Mubarok, Muhammad Syahrul Muh. Arsul Haq Sulthan Muhammad Agus Muhammad Agus Futuhul Ma'wa Muhammad Alif Rizky H Muhammad Andry Muhammad Dwiky Nugraha Muhammad Yassir namirudin Muhtadin Naila Joya Nazlah Azzahra Neng Dina Agustina Nisa, Asri Ainun Nisrina Alya Salsabila Nugraha, Irsyad Nur Hidayah Nur Jannah Nur Risal, Andi Akram Nuriessa Aputri, Farah Nurmina Nurmina, Nurmina Pamungkas, Barolym Tri Pragustiandi, Guntur Purwaningtyas, Esti Putra, Purniadi Putri, Cyntia Sabarani Rachmanita, Risse Entikaria Radial Anwar, Radial Rahmadania Affelia Dianto Rahman Ismail M.S, Rahman Ismail Rahmat Pratama, Rahmat Rahmat, Pratama Ramdhan, William Ratih Wijayanti, Ratih Rian Firmansyah Risaldi, Muhammad Riva Rivanti Rizki Rahmawati, Rizki Rosyita Nur Syafa'ah Rubi Awalia, Rubi Rudy Safriyah Safriyah SANTOSO SANTOSO Saputra, Mochammad Yamin Sendi Ibnu Hilmiawan Setia Budi Setyaningsih, Eka Wahyu Shafwan Aulia Rahman Siti Nurhalisa Sri Ayu Wandira St Susi Astika Sari Sugianti, Wiwik Wahyu Suhendra Suhendra Syahirani, Siti Thamrin Kamaruddin, Thamrin Untung Usada Vera Wati Viena Eka Yuadianti Wanda Tri Agustin Wulandari, Lucia Putri Yadi Oktariansyah Yuanita Windusari Zubaidah