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Journal : Journal of Parasite Science

The Prevalence of Gastrointestinal Tract Helminthiasis Through Stool Examination in Cattle at Benowo Landfill Surabaya Ratih Prajnya Paramitha; Rahaju Ernawati; Setiawan Koesdarto
Journal of Parasite Science (JoPS) Vol. 1 No. 1 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (609.829 KB) | DOI: 10.20473/jops.v1i1.16218

Abstract

Tujuan penelitian ini adalah untuk mengetahui prevalensi cacing, untuk mengetahui berbagai jenis telur cacing saluran cerna dan jenis umur terhadap infeksi cacing saluran cerna pada sapi di tanah Benowo, Surabaya. Sampel tinja diambil dari TPA sebanyak 41 sampel. Pemeriksaan tinja dilakukan dengan metode asli, sedimentasi sederhana dan pelapisan Fulleborn. Hasil penelitian menunjukkan bahwa 30 sampel positif menentang gastrointestinal, ini menunjukkan prevalensi cacing adalah 73%. Jenis telur cacing adalah kelas Nematode, yaitu Oesophagostomum sp., Trichostrongylus sp., Bunostomum sp. , Mecistocirrus digitatus, Trichuris sp dan Toxocara vitulorum.Menurut analisis statistik, dapat disimpulkan jenis dan jenis kelamin tidak dapat dihitung terhadap infeksi cacing.
Prevalence of Trypanosomiasis of Wild Rats (Rattus sp.) in Banyuwangi Zhaza Afililla; Lucia Tri Suwanti; Sri Agus Sudjarwo; Setiawan Koesdarto; Hani Plumeriastuti
Journal of Parasite Science (JoPS) Vol. 1 No. 2 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (416.043 KB) | DOI: 10.20473/jops.v1i2.16283

Abstract

The aim of this research was to investigate the number of prevalence of Trypanosomiasis of wild rats in Banyuwangi. Sixty wild rats were trapped from human residence, markets and rice fields in Banyuwangi. Rat`s  blood smear was stained by Giemsa. The result show that one (1.67%.) of 60 blood sample was appear Trypanosoma sp..  The prevalence of Trypanosomiasis of wild rats in Banyuwangi was 1.67%.
Effect of Garlic (Allium sativum) Infusion On The Time of Death of Ascaridia galli Ida Yuniarti; Suzanita Utama; Setiawan Koesdarto; Lucia Tri Suwanti; Ngakan Made Rai Widjaja
Journal of Parasite Science (JoPS) Vol. 1 No. 2 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (416.655 KB) | DOI: 10.20473/jops.v1i2.16284

Abstract

Infectious disease is a major problem for animal husbandry. One of infectious disease is caused by Ascaridia galli (A. galli). Ascaridiasis occurs in either broiler or layer that caused decrease of meat and egg productions. Garlic (Allium sativum) contain alicin  that have the ability to kill parasites.This research was conducted to determine the effect of garlic on time of death of A. galli. In this research used A. galli with length 7-11 cm regardless the sex. The treatment used local (Indonesian garlic), kating and single garlic with concentration of 39.50%, 62.40% and 98.60%. The negative control used NaCl physiology (0.9 %). The result showed that all the treatment group can shortened the time of death of A. galli compared to control. The time of death of A. galli on local garlic infusion with 39.50 % concentration was 6.24 hours, on 62.40 % concentration was 4.94 hours and on 98.60 % concentration was 3.97 hours, kating garlic infusion on 39.50 % concentration was 5.08 hours on 62.40 % concentration was 3.81 hours, on 98.60 % concentration was 2.96 hours, single garlic infusion on 39.50 % concentration was 5.4 hours, on 62.40 % concentration  was 3.86 hours, on 98.60 % concentration was 3.83 hours. The best treatment was kating garlic on 98.60% concentration because give shortest time of death.
Anthelmintic Activity of Basil Leaves (Ocimum sanctum Linn.) Infusion Against Ascaris suum In Vitro Ogen Sea; Mas'ud Hariadi; Setiawan Koesdarto; Kusnoto Kusnoto; Ngakan Made Rai Widjaja
Journal of Parasite Science (JoPS) Vol. 1 No. 2 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (586.215 KB) | DOI: 10.20473/jops.v1i2.16285

Abstract

Ascaris suum is a parasite nematode that causes infection in swines with high prevalence rates in host populations and usually associated with liver damages called “milk spots” caused by larvae migration, resulting in organ condemnation. Basil leaves (Ocimum sanctum) phytochemical constituents contains flavonoid, phenol and tannin. Tannins and phenolics are known to interfere with the energy generation in helminth parasites by uncoupling oxidative phosphorylation and also bind to free proteins in the gastrointestinal tract of host animal or glycoprotein on the cuticle of the parasite and leading to death. This study was aimed to determine the activity of basil leaves (Ocimum sanctum) infusion in several concentrations against A. suum in vitro. This research used six treatments and four replications. This research used 10 A. suum in each treatment with four replication. The observations were done at 12, 18, 24, 30 and 36 hours in an incubator at 37oC. Based on the data analysis, basil leaves (Ocimum sanctum) infusion has anthelmintic activity against A. suum in vitro. The greater of the concentration and the longer of time of immersion, will make the death percentage of A. suum become higher. Concentration of basil leaves (Ocimum sanctum) infusion 15% is the effective concentration that can kill 100% of the A.suum during 36 hours of immersion.
The Anthelmintic Activity of Ethanol Extract of Basil Leaves (Ocimum americanum Linn.) Againt Haemonchus contortus Death in Vitro Rari Noviana; Chairul Anwar Nidom; Agus Sunarso; Setiawan Koesdarto; Sri Mumpuni Sosiawati; Iwan Sahrial Hamid
Journal of Parasite Science (JoPS) Vol. 1 No. 2 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (455.906 KB) | DOI: 10.20473/jops.v1i2.16289

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui daya anthelmintik dari ekstrak etanol daun kemangi (Ocimum americanum Linn.) terhadap mortalitas cacing Haemonchus contortus secara in vitro. Sediaan ekstrak dibuat dengan konsentrasi (berat/volume) 2,5%, 5%, 7,5%, dan 10%. Untuk kontrol positif digunakan PBS dan DMSO sedangkan untuk kontrol negatif digunakan Albendazole 10 mg/ml. Sebanyak 10 ekor cacing Haemonchus contortus betina direndam dengan berbagai perlakuan dalam cawan petri. Masing-masing perlakuan diulang sebanyak 4 kali. Pengamatan dilakukan setiap 1 jam sekali selama 6 jam untuk melihat apakah cacing masih hidup atau sudah mati. Hasil dari penelitian ini menunjukkan bahwa ekstrak daun kemangi memiliki efek anthelmintik terhadap cacing Haemonchus contortus secara in vitro. Makin tinggi konsentrasi ekstrak, maka daya anthelmintiknya juga semakin tinggi. Pada ekstrak konsentrasi 10% memiliki kemampuan anthelmintik yang hampir sama dengan Albendazole.
The Prevalance and Helminth Infection Degree of Gastrointestinal in Layer Duck Located in Keper and Markolak Kramat Village District of Bangkalan Regency of Bangkalan Ana Amaliah; Indah Norma Triana; Poedji Hastutiek; Setiawan Koesdarto; Lucia Tri Suwanti; Soeharsono Soeharsono
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (423.067 KB) | DOI: 10.20473/jops.v2i1.16376

Abstract

Tujuan dari penelitian ini adalah untuk menentukan prevalensi dan derajat infeksi cacing saluran pencernaan pada itik petelur di dua kondisi lingkungan yang berbeda. Sampel feses diperiksa dengan metode sedimentasi dan metode apung kemudian dilanjutkan dengan menghitung telur cacing per gram tinja dengan metode Mc Master. Hasil menunjukkan bahwa 22,9% (8/35) itik petelur di Dusun Keper terinfeksi oleh Capillaria sp., dan Echinostoma revolutum, dengan rata-rata derajat infeksi sebsar 52,50 ± 41,662, sedangkan 31,4% (11/35) itik petelur di Dusun Markolak terinfeksi oleh Capillaria sp., Echinostoma revolutum, dan cacing dari kelas cestoda, dengan rata-rata derajat infeksi sebesar 155,45 ± 166,395. Infeksi bersifat tunggal maupun campuran. Analisis terhadap prevalensi dan derajat infeksi cacing saluran pencernaan itik petelur menunujukkan hasil yang tidak signifikan (p>0,05) antara Dusun Keper dan Dusun Markolak.
The Effect of Calcium Hydroxideon Mortality of Ascaridia galli In Vitro Inggarsetya Syah Audini; Setiawan Koesdarto; Ira Sari Yudaniayanti; Setiawati Sigit; Sri Mumpuni Sosiawati; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (698.427 KB) | DOI: 10.20473/jops.v2i1.16377

Abstract

Ascaridiasis can be happened in either broiler or hens that can caused decrease of meat and egg productions. Ascaridiasis is a parasite that is most often found in poultry and cause high economic losses. This research was conducted to determine the anthelmintic effect of calcium hydroxide [Ca(OH)₂] on mortality of Ascaridia galli in vitro. In this research used Ascaridia galli with length 7-11 cm without differentiating their sex. The concentrations of calcium hydroxide [Ca(OH)₂] for immersion the Ascaridia galli were 0,01%, 0,02%, 0,025% and 0,03% as treatment group, respectively. The negative control used aquadest. Observation death A.galli worm and analysis of the data at the 2 hour, 4 hour, 6 hour, 8 hour, 10 hour and 12 hour. The result showed that the increase of concentration increased mortality of Ascaridia galli. Percentage of death worm A.galli at the 12th hour on concentration ,01%, 0,02%, 0,025% and 0,03% was 20%, 55%, 75% and 100%. ANOVA test showed significant difference between control and treatment group (p<0.05). Duncan multiple range test was seen the best treatment was the biggest mortality in concentration of 0,03%.
Anthelmintic Activity Ethanol Extract of Ocimum sanctum Linn. Leaves Against Ascaridia galli In Vitro Vanna Lidya Kharisma; Setiawan Koesdarto; Koesnoto Supriandono; Lucia Tri Suwanti; Sri Agus Sudjarwo; Kusnoto Kusnoto
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (834.409 KB) | DOI: 10.20473/jops.v2i1.16380

Abstract

The aims of this research are to determine concentration, exposure time, interaction between concentration and exposure time of ethanol extract of Ocimum sanctum Linn. Leaves to cause death toward Ascaridia galli in vitro, and the value of LC50 and LC90 ethanol extract of Ocimum sanctum Linn. Leaves. Research design that has been used in the research was completely randomized design. This research used 200 samples of Ascaridia galli with length 7-11 cm without differentiating their sex. The concentration ethanol extract of Ocimum sanctum Linn. leaves were 1.25%, 2.5%, 5%, 10%. The control was using CMC-Na 0.5%. Each treatment then being replicated four times. The observation and recording of dead worm were done at 0, 3, 6, 12 and 24 hours. Ascaridia galli were declared dead if there was no movement while disturbed by anatomy tweezers and dipped in slightly warm water (50ºC). The obtained data was analyzed using Anova Factorial and continued with Duncan Multiple Range Test by SPSS for Windows 22. The result were 10% concentration and exposure time for 24 hours caused the most mortality toward Ascaridia galli. Interaction between concentration and exposure time resulted 10% concentration ethanol extract of Ocimum sanctum Linn. leaves in 24 hours caused the most mortality towards Ascaridia galli. Probit analysis was used to calculate the LC50 and LC90 of Ocimum sanctum Linn. leaves. The results were LC50 ethanol extract of Ocimum sanctum Linn. leaves at 6 hours was 14.8%, at 12 hours was 4.8% and at 24 hours was 3.0% and the LC90 at 24 hours was 9.1%.
Identification of Worm In Digestive Tract of Consumed Cattle Egret (Bubulcus ibis) In Ujung Pangkah, Gresik Nisa’ Rachmaningtyas Putri; E Djoko Poetranto; Nunuk Dyah Retno Lastuti; Setiawan Koesdarto; Bambang Sektiari Lukiswanto; Kusnoto Kusnoto
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (476.189 KB) | DOI: 10.20473/jops.v2i1.16382

Abstract

Ujung Pangkah, Gresik was became the most habitat of fowls such as Cattle egret (Bubulcus ibis). The aim of this research were to find out the type of worm that infected digestive tract of Cattle egret (Bubulcus ibis) in Ujung Pangkah, Gresik. The examination of worm in digestive tract was done by dissection and fecal examination. The identification based on morphological of the eggs and worms. The samples of digestive tract organ collected from restaurant that preserve Cattle egret as a main menu. Whereas the fecal sample collected by swab from the intestine. Cattle egret (Bubulcus ibis) has positive infection by worm parasite if there were worm or egg inside the digestive tract. Fecal samples were examined by native, sedimentation, coconut-sugar floating method and EPG counting with Lucient Brumpt. And the worm morphology from dissection of digestive tract would be identify with source book, journal, and discussed with supervisor. The result showed that 20 of digestive tract dissection and 20 fecal samples, there were three types of worm, Contracaecum sp., Cosmocephalus sp., Capillaria sp. and egg of Contracaecum sp. with 200 EPG
Efectiveness of Ocimum sanctum Linn. Leaves Ethanol Extract on Mortality Raillietina echinobothrida In Vitro Jessica Giovani; Setiawan Koesdarto; Mirny Lamid; Sri Agus Sudjarwo; Suryo Kuncorojakti
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (323.403 KB) | DOI: 10.20473/jops.v2i1.16383

Abstract

The aims of the research are to prove the anthelmintic effects of Ocimum sanctum Linn. leaves ethanol extract on Raillietina echinobothrida in vitro and to know the value of LC50 and LC90of Ocimum sanctum Linn. leaves ethanol extract. The research used completely randomized design. The research had five treatments and each treatment was done in four replications. The research used 5 Raillietina echinobothrida adult worms for each treatment and all replications. The observation and the recording were done at the first hour until the sixth hour of the research. The Raillietina echinobothrida adult worms were noted dead if there was no movement when they were disturbed by anatomic tweezer, the death of Raillietina echinobothrida were confirmed when they dipped mildly in warm water (50°C) and they showed no movements. The acquired data was analyzed using ANOVA and continued with LSD (5%). The results were Ocimum santum Linn. ethanol extract has the anthelmintic activity in 1.25%, 2.5%, 5% and 10% concentrations. The LC50 and LC90 was analyzed using probit analysis. The results of LC50 calculation were 7.8% at the third hour, 6% at the forth hour, 3.2% at the fifth hour, 2.5% at the sixth hour and the results of LC90 were 6.5% at the fifth hour, 5.2% at the sixth hour. The higher concentration of the extract, the higher anthelmintic activity worked.
Co-Authors Adiacahya, Eren Adiana Mutamsari Witaningrum Agus Wijaya Akbar Haryo Widhi Trismiharto Aksono HP., Eduardus Bimo Aldi Hamdani Amelia Dwita Safitri Ana Amaliah Anita Nur Aida Aida Annisa Novia Damayanti APRIL HARI WARDHANA Arum Puspitasari Aryaloka, Suhita Ayuti, Siti Rani Azizatun Nimah Bagaskara, Ryan Bambang Sektiari Lukiswanto Boedi Setiawan Bryan Ahmad Affan Lubis BUDI UTOMO Budiarto Budiarto Budiarto Budiarto Dadik Rahardjo, Dadik Dewi Mariyam Dhimar Maulud Dyahningrum Dian Ayu Permatasari Didik Handijatno Didik Handijatno Djoko Poetranto E Djoko Poetranto Eka Pramyrtha Hestinah Emy Koestanti Sabdoningrum Endang Suprihati Eny Coolfina Simarmata Felita Widyaningsih Firman Hadi Fanani Galuh Swa Ardhya Gunanti Mahasri Hana Eliyani Hani Plumeriastuti Hasib, Abdullah Heni Puspitasari Hermin Ratnani Herry Agoes Hermadi Ida Yuniarti Indah Norma Triana, Indah Norma Indira Vania Carisya Inggarsetya Syah Audini Ira Sari Yudaniayanti Iwan Sahrial Hamid Iwan Sahrial Hamid Iwan Sahrial Hamid jayawardhana, andi Jessica Giovani Karina Rachmawati Khairullah, Aswin Rafif Khairunnisa, Hanifa Khansa Khalissa Farah Alifia Kismiyati , Koesnoto Supranianondo Koesnoto Supriandono Kuncoro Puguh Santoso Kuncorojakti, Suryo Kurnianto, Muhammad Aqil Kurniawati, Dyah Ayu Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Lutfiah Annisa Billa Mafruchati, Maslichah Mas’ud Hariadi Melani Anggraini Melanie Aulia Ashfiyah Meles, Dewa Ketut Mirni Lamid Mirny Lamid Mochamad Lazuardi Mohamad Safri Sauqi Moses, Ikechukwu Benjamin Muchammad Yunus Mufasirin Muhammad Rofi&#039; Prasetya Muhammad Rofi' Prasetya Mustofa Helmi Effendi Nenny Harijani Ngakan Made Rai Widjaja Nidom, Chairul Anwar Nila Murodah Nisa’ Rachmaningtyas Putri Nove Hidajati Novia Intan Kurnia Nunuk Dyah Retno Lastuti Nunuk Dyah Retno Lastuti Nurhusien Yimer Ogen Sea Poedji Hastutiek Poetranto, Emmanuel Djoko Praja, Ratih Novita Prasetya, Muhammad Rofi' Pratiwi, Arif Primarizky, Hardany Pudji Srianto Putri, Valerie Brilianda Rahadju Ernawati Rahaju Ernawati Rahardjo, Adi Prijo Rahmi Sugihartuti Rari Noviana Ratih Prajnya Paramitha Rekasni Adallin A/P Morgan A/P Morgan Rimayanti Rimayanti Risky Aprillian, Risky Rochmah Kurniasanti Santosa, Gusti Ayu Illiyin Putri Sari, Aulia Puspita Sari, Putri Desi Wulan Sarmanu, Sarmanu Septian Hakim Susantoputro Sesa Puput Febriyanti Setiawati Sigit Soeharsono Soeharsono Soelih Estoepangestie, Agnes Theresia Sofie Adi, Keren Jayanti Sri Agus Sudjarwo Sri Mumpuni Sosiawati Sri Mumpuni Sosiawati Sunarso, Agus Suwarno Suwarno Suzanita Utama Syah Putra, Akyun Rozaqi Tanjung, Aldi Gusnizar Rizaldy Tita Damayanti Lestari Tjuk Imam Restiadi Trifena Pristi Anindyta Tyasningsih, Wiwiek Tyasningsih, Wiwik Vanna Lidya Kharisma Vindo Rossy Pertiwi Virgy Dzakina Fahrezy Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widya Paramita Lokapirnasari Yeni Dhamayanti Yudhana, Aditya Yulianna Puspitasari Yunus, Muchammad Yunus, Muchammad Zhaza Afililla