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Penetapan Kadar Fenolik Total dan Uji Aktivitas Antioksidan Fraksi dari Ekstrak Etanol Daun Cempedak (Artocarpus integer) dengan Metode DPPH Rizki, Muhammad Ikhwan; Sari, Anna Khumaira; Kartika, Dewi; Khairunnisa, Amalia; Normaidah
MPI (Media Pharmaceutica Indonesiana) Vol. 4 No. 2 (2022): DECEMBER
Publisher : Fakultas Farmasi, Universitas Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24123/mpi.v4i2.4937

Abstract

Daun cempedak (Artocarpus integer) dimanfaatkan secara tradisional oleh masyarakat Kalimantan Selatan dalam pengobatan. Golongan fenolik terkandung dalam ekstrak daun cempedak dan memiliki kemampuan sebagai antioksidan. Fraksi dari ekstrak daun cempedak belum pernah ditetapkan kadar fenolik total dan akivitas antioksidannya. Penelitian ini bertujuan untuk mengetahui fraksi dari ekstrak daun cempedak yang mengandung kadar fenolik dan aktivitas antioksidan tertinggi. Penelitian dimulai dengan pengeringan daun segar menggunakan lemari pengering, pembuatan serbuk simplisia, proses ekstraksi secara maserasi, pengeringan ekstrak dengan lemari pengering, dan proses fraksinasi dengan pelarut n-heksan, etil asetat dan aquadest. Penetapan kadar fenolik total dapat dilakukan dengan reagen Folin-Ciocalteu dan menggunakan baku pembanding asam galat. Aktivitas antioksidan dilakukan menggunakan metode DPPH berdasarkan nilai IC50 dan dengan pembanding kuersetin. Kadar fenolik total pada fraksi n-heksan 9,352 ± 0,113% b/b, fraksi etil asetat 12,595 ± 0,415% b/b, dan fraksi aquadest 9,992 ± 0,485% b/b ekivalen asam galat. Fraksi n-heksan, etil asetat, dan aquadest masing-masing memiliki nilai IC50 sebesar 89,192 ± 2,91 bpj (kuat), 64,754 ± 2,803 bpj (kuat), dan 82,247 ± 23,034 bpj (kuat). Kesimpulan dari penelitian ini bahwa fraksi etil asetat memiliki kadar fenolik total tertinggi dan aktivitas antioksidan paling kuat dibandingkan fraksi n-heksan dan fraksi aquadest dari ekstrak daun cempedak. Cempedak (Artocarpus integer) leaves are traditionally used by people in South Kalimantan as medicine. The phenolic group contained in cempedak leaf extract has known as an antioxidant. The fraction of cempedak leaf extract has never been determined for total phenolic content and antioxidant activity. This study aimed to determine the fraction of cempedak leaf extract that contains the highest levels of phenolic and antioxidant activity. The study started with drying of fresh leaves using a drying cabinet, simplicia powder preparation, maceration extraction, extract drying, and fractionation with n-hexane, ethyl acetate and aquadest as solvents. Determination of total phenolic content was obtained with Folin-Ciocalteu reagent and gallic acid as a standard for comparison. Antioxidant activity was measured using the DPPH method based on the IC50 value and comparison with quercetin. The total phenolic content of the n-hexane, ethyl acetate and aquadest fraction were 9.352 ± 0.113% w/w, ethyl acetate fraction 12.595 ± 0.415% w/w, and aquadest fraction 9.992 ± 0.485% w/w equivalent gallic acid, respectively. The n-hexane, ethyl acetate, and aquadest fractions showed IC50 values of 89.192 ± 2.91 ppm (strong), 64.754 ± 2.803 ppm (strong), and 82.247 ± 23.034 ppm (strong), respectively. The conclusion of this study was that the ethyl acetate fraction had highest total phenolic content and strongest antioxidant activity compared to n-hexane and aquadest fraction from cempedak leaf extract.
The Analgesic Effect and Toxicity of Red and White Ginger on Mus Musculus with the Acetate Writhing Test Dewi, Vonny Khresna; Barkinah, Tut; Kirana, Rita; Rizki, Muhammad Ikhwan
Medical Laboratory Technology Journal Vol. 10 No. 2 (2024): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i2.611

Abstract

Based on data from the World Health Organization in 2020, the incidence of dysmenorrhea was 1,769,425 (90%) women who suffered from dysmenorrhea, with 10-16% suffering from severe dysmenorrhea. The problem of pain is very disruptive to activities, so prevention and treatment of pain must be a concern. Ginger is a natural ingredient that grows in South Kalimantan and is empirically used to treat pain. The study aimed to determine the analgesic effects and toxicity of red and white ginger from Banjarmasin and Banjar Regency. The nutrient content profile of the soil where the ginger grew was analyzed, and it was determined by nitrogen content, cation exchange capacity, and soil pH. The content of active compound groups was analyzed using specific reagents to determine the presence of active compounds. Determination of total phenolic levels using a colorimetric method using a UV-Vis spectrophotometer. The instant ginger formula was optimized with organoleptic test result parameters. Acute toxicity tests using male Wistar rats with observations for 14 days. Analgesic tests were conducted on Mus musculus using the acetic acid writhing test. The results showed that the highest nitrogen (1,34%), cation exchange capacity (29.22 me/100 g), and soil pH levels (6,89) were in the soil where the ginger samples from Banjar Regency grew. The chemical compound content in all gingers contained the same phenolics, flavonoids, tannins, saponins, and terpenoids. The levels of marker compounds in succession from Banjarmasin red ginger, Banjarmasin white ginger, Banjar Regency red ginger, and Banjar Regency white ginger were 7.43%, 6.26%, 8.52%, and 7.12%, respectively. The results of formula optimization showed that all gingers had a sweet and spicy taste, a distinctive odor, powder form, and a reddish-brown color in red ginger and pale yellow in white ginger. The toxicity test results showed that at doses of 300 mg/Kg BW and 2000 mg/Kg BW, there was no toxic effect on the administration of ginger extract. Analgesic activity showed that all gingers had an analgesic effect at a dose of 200 mg/Kg BW. The analgesic effects from the strongest to the lowest were red ginger from Banjar Regency, red ginger from Banjarmasin, white ginger from Banjar Regency, and white ginger from Banjarmasin. This study concludes that red ginger from Banjar Regency has the most potent analgesic activity and no toxicity for all ginger.
Upaya Peningkatan Pengetahuan Terhadap Permasalahan Penyakit Hipertensi di Desa Tambak Baru Ilir Kabupaten Banjar Rizki, Muhammad Ikhwan; Triyasmono, Liling; Hadi, Samsul; Normaidah, Normaidah; Ratnapuri, Prima Happy; Izma, Hayatun; Mahdi, Nur; Malahayati, Siti; Buih, Putri Helena Junjung; Agustina, Ni Kadek Ayu
Jurnal Pengabdian Masyarakat Panacea Vol 2, No 4 (2024): Jurnal Pengabdian Masyarakat Panacea
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jpmp.v2i4.13964

Abstract

Penyakit tidak menular (PTM) merupakan suatu penyakit yang tidak bisa menular dari satu orang ke orang lainnya. Salah satu PTM yang cukup banyak diderita oleh masyarakat adalah hipertensi. PTM dapat disebabkan pola hidup maupun pola makan yang tidak berimbang menyebabkan angka kejadian penyakit ini terus meningkat dari waktu ke waktu. Tingkat penyakit hipertensi pada masyarakat Desa Tambak Baru Ilir masih tinggi dan diketahui masyarakat desa ini masih memiliki pengetahuan yang minim termasuk keterampilan pengelolaan manajemen hipertensi. Tujuan pengabdian yang telah dilakukan yaitu meningkatkan pengetahuan masyarakat dengan penyakit hipertensi di Desa Tambak Baru Ilir. Kegiatan pengabdian dilakukan pada bulan agustus 2024. Secara umum kegiatan pengabdian diawali dengan sosialisasi kegiatan pada kepala desa. Selanjutnya tim pengbadian masyarakat membuat media edukasi berupa leaflet tentang hipertensiMasyarakat yang akan diberikan edukasi sebelumnya diminta mengisi pretest, selanjutnya diakhir kegiatan mengerjakan posttest. Hasil kegiatan pengabdian masyarakat menunjukkan pengabdian masyakat diikuti oleh 13 orang yang terdiri atas perempuan, usia 38-70 tahun, dan pekerjaan didominasi ibu rumah tangga. Nilai pretest dari skala 0-10 yang didapatkan rata-rata 7,08 dan nilai posttest rata-rata 9,23. Kesimpulannya yaitu terdapat peningkatan pengetahuan masyarakat tentang hiprtensi secara signifikan sebelum dan sesudah edukasi pada kegiatan pengabdian masyarakat di Desa Tambak Baru Ilir. Kata Kunci: Edukasi, Hipetensi, Masyarakat, Pengetahuan, Banjar Non-communicable diseases (NCDs) cannot be transmitted from one person to another. One of the NCDs that is quite common in the community is hypertension. NCDs can be caused by an unbalanced lifestyle or diet, causing the incidence of this disease to continue to increase over time. The level of hypertension in the Tambak Baru Ilir village community is still high and it is known that the people of this village still have minimal knowledge, including hypertension management skills. The purpose of the community service that has been carried out is to increase public knowledge about hypertension in Tambak Baru Ilir village. Community service activities were carried out in August 2024. In general, community service activities began with the socialization of activities to the village head. Furthermore, the community service team made educational media through leaflets about hypertension. The community who would be given education were previously asked to fill out a pretest, then at the end of the activity, they did a posttest. The results of community service activities showed that community service was attended by 13 people consisting of women, aged 38-70 years, and jobs dominated by housewives. The pretest score on a scale of 0-10 obtained an average of 7.08 and the average posttest score was 9.23. The conclusion is that there was a significant increase in public knowledge about hypertension before and after education in community service activities in Tambak Baru Ilir village.
Antimicrobial Activity of Ethanol Extract of Sea Pandan Leaves (Pandanus odorifer) Against Staphylococcus aureus and Escherichia coli Rizki, Muhammad Ikhwan; Sari, Anna Khumaira; Rahma, Salsabila Fadiya; Rahmatullah, Satrio Wibowo; Izma, Hayatun
Jurnal FARMASIMED (JFM) Vol 7 No 1 (2024): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v7i1.2280

Abstract

Sea pandan (Pandanus odorifer) is used in traditional medicine in India. Sea pandan is efficacious in treating pain, parasites, digestive, liver, and cognitive disorders. Sea pandan is found in coastal areas in South Kalimantan. Sea pandan contains flavonoid compounds that have the potential to be antimicrobials. This study aimed to determine the antimicrobial activity of sea pandan leaf extract. The study began with sample preparation, which included washing the leaves, drying them using an oven, refining the particle size, extracting 70% ethanol, and evaporating the solvent until a thick extract was obtained. Total flavonoid levels in sea pandan leaf extract were determined using the colourimetric method using quercetin standards. Antimicrobial testing was conducted using the diffusion method at concentrations of 30%, 40%, and 50% against Staphylococcus aureus and Escherichia coli. The positive control used was chloramphenicol. The determination of the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values ??was carried out using the dilution method. The results showed that sea pandan leaf extract contains phenolics, flavonoids, saponins, steroids, and tannins. The total flavonoid content in sea pandan leaf extract is 10.01% w/w equivalent of quercetin. The antimicrobial activity of sea pandan leaf extract against Staphylococcus aureus bacteria is 10.15 - 25.36 mm, while against Escherichia coli in the range of 8.15 - 23.16 mm. Ethanol extract of sea pandan leaves at a concentration of 50% is included in the very strong category in inhibiting Staphylococcus aureus and Escherichia coli. Ethanol extract of sea pandan leaves has an MIC value of 7.5% on Staphylococcus aureus and 15% on Escherichia coli. MBC of sea pandan extract is 15% on Escherichia coli bacteria. This study concludes that the ethanol extract of sea pandan leaves has antimicrobial activity against Staphylococcus aureus (bacteriostatic) and Escherichia coli (bactericidal).
Antioxidant Activity and Marker Compound Levels of Cempedak Leaf (Artocarpus integer) Extract in Different Solvent Variations Sari, Anna Khumaira; Rizki, Muhammad Ikhwan; Azhara, Desiya Damayanti; Fadlilaturrahmah, Fadlilaturrahmah
Tropical Health and Medical Research Vol. 6 No. 2 (2024): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35916/thmr.v6i2.123

Abstract

Cempedak (Artocarpus integer) is known to contain phenolic compounds. Phenolic compounds are secondary metabolites of plants included in marker compounds that act as natural antioxidants because they have hydroxyl groups that can reduce free radicals. Plants containing phenolic compounds are known to have strong antioxidant activity. This study aimed to determine the antioxidant activity and levels of marker compounds from ethanol extracts of cempedak leaves in various solvent variations. Cempedak leaves were dried in an oven and then powdered. Cempedak leaf powder was extracted using methanol, 70% ethanol, 96% ethanol using the maceration method, and distilled water was extracted using the boiling method. The extract was tested for antioxidant activity using the DPPH (1,1-diphenyl-2-picryl-hydrazyl) method based on the IC50 value. Determination of marker compound levels using the Folin-Ciocalteu reagent with a gallic acid comparator using a UV-Vis spectrometer instrument. The total phenolic content determination test results were expressed as gallic acid equivalents per gram of extract (GAE/g extract). The results of the antioxidant activity test showed the IC50 value of the methanol extract was 84.48 ppm (strong), the distilled water extract was 54.74 ppm (strong), the 70% ethanol extract was 72.21 ppm (strong), and the 96% ethanol extract was 24.55 ppm (very strong). The results of the marker compound determination showed the total phenolic content of the methanol extract was 28.22% w/w, the distilled water extract was 22.59% w/w, the 70% ethanol extract was 29.55%, and the 96% ethanol extract was 31.14% w/w. It can be concluded that the cempedak leaf extract extracted with 96% ethanol has very strong antioxidant properties and the highest marker compound content among other solvents. For further analysis, 96% ethanol solvent can be used for the cempedak leaf extraction.
Kacip Fatimah (Labisia pumila) as Natural Sunscreen: Analysis of Flavonoid Content and Ultraviolet Protection Ability Rahmatullah, Satrio Wibowo; Rizki, Muhammad Ikhwan; Sari, Anna Khumaira; Sari, Fitri Novita; Fadlilaturrahmah, Fadlilaturrahmah; Junjung Buih, Putri Helena
Tropical Health and Medical Research Vol. 7 No. 1 (2025): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35916/thmr.v7i1.132

Abstract

The cause of melanoma cancer is exposure to high-intensity ultraviolet rays from sunlight. Kacip Fatimah (Labisia pumila) utilizes the people of the interior of Kalimantan to protect their skin from sunlight by attaching leaves to the face or skin. Kacip Fatimah (Labisia pumila) contains flavonoid compounds, which are thought to impact these various properties. This study aimed to determine the ultraviolet protection ability and total flavonoid levels of Kacip Fatimah (Labisia pumila) leaves. Kacip Fatimah (Labisia pumila) leaves originating from Central Kalimantan were cleaned, dried in an oven, ground, and then extracted with air solvents, 70% ethanol, and 96% ethanol. Flavonoid identification was carried out with specific reagents, and total flavonoid levels were determined by colorimetric method using quercetin as a comparison. The ultraviolet protection ability of Kacip Fatimah (Labisia pumila) leaf extract was measured using a UV-Vis Spectrophotometer with the Sun Protection Factor parameter. The results showed the presence of flavonoids in the third extract. The total flavonoid content of air extract, ethanol 70%, and ethanol 96% from Kacip Fatimah (Labisia pumila) leaves were obtained respectively at 1.69%, 2.15%, and 2.38%. The most extracted flavonoids were in ethanol 96%solvent. The ultraviolet protection ability at a concentration of 100 - 500 ppm from air extract was in the range of 2.80 - 7.99, in ethanol 70% extract in the range of 5.69 - 38.39, while in ethanol 96% extract in the range of 8.76 - 49.07. The highest ultraviolet protection was in 96% ethanol extract from Kacip Fatimah (Labisia pumila) leaves. The results showed that Kacip Fatimah (Labisia pumila) leaves can provide ultraviolet protection with the content of active compounds of the flavonoid group. The research results can be scientific evidence for utilizing and developing products from Kacip Fatimah (Labisia pumila).
Characteristics and Antioxidant Activity of Limau Kuit Peel (Citrus hystrix) Extract with Variation of Extraction Solvent Rizki, Muhammad Ikhwan; Agustina, Ni Kadek Ayu; Sari , Anna Khumaira; Rahmatullah, Satrio Wibowo; Isnani, Nazhipah
Medical Laboratory Technology Journal Vol. 11 No. 1 (2025): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v11i1.650

Abstract

Limau kuit (Citrus hystrix) has been traditionally utilized for its therapeutic properties, particularly the antioxidant potential of its peel. Extraction is preferred for medicinal use, with solvent selection playing a critical role in determining the yield and concentration of bioactive compounds. This study aimed to assess the characteristics and antioxidant activity (IC₅₀ value) of Limau Kuit peel extract extracted with three different solvents. The research methods, starting with sample preparation, were carried out by drying Limau Kuit peel, then extraction using three different solvents: distilled water, ethanol 70%, and ethanol 96%. Extraction with distilled water solvent used the infusion method, while the ethanol solvent used the maceration method. The third extract was then characterized based on the parameters of percent yield, organoleptic testing, phytochemical screening, chromatography profile, and determination of total flavonoid levels. Antioxidant activity testing on the third extract using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. The study results showed that the third extract had the same shape, color, aroma, taste, and compound content: flavonoids, phenolics, alkaloids, and terpenoids. The results of thin-layer chromatography also showed no difference in the third extract. The characteristic differences were found in the parameters of percent yield and total flavonoid content.  The highest total flavonoid content of Limau Kuit (Citrus hystrix) peel extract was obtained using 70% ethanol (11.47% w/w), followed by 96% ethanol (6.39% w/w), and distilled water (2.82% w/w). Antioxidant activity, evaluated using the IC₅₀ value as the parameter, showed that the 70% ethanol extract exhibited the strongest activity (21.26 ppm, very strong), followed by the 96% ethanol extract (31.87 ppm, very strong), and distilled water extract (70.91 ppm, strong). This study concludes that 70% ethanol is the most effective solvent for extracting antioxidant-rich compounds from Limau Kuit peel, supporting its potential use in herbal formulations.
Penyuluhan Kesehatan Mental Pada Mahasiswa Tingkat Akhir Program Studi Farmasi Fakultas MIPA Universitas Lambung Mangkurat Hayatun Izma; Satrio Wibowo Rahmatullah; Muhammad Ikhwan Rizki; Anna Humaira
SAFARI :Jurnal Pengabdian Masyarakat Indonesia Vol. 3 No. 1 (2023): Januari : Jurnal Pengabdian Masyarakat Indonesia
Publisher : BADAN PENERBIT STIEPARI PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56910/safari.v3i1.361

Abstract

Mental health is as important as physical health. Final year students are inclined for mental health disorders associated with finishing their final project. This activity is to increase student’s knowledge and awareness of mental health so it can stimulate a healthy mental of students who are finishing final projects. This activity began with a pretest session to measure participant’s initial knowledge about mental health, presentation using experiental methods, discussion, sharing and Q&A session, and top it off with a posttest session. The results of this activity showed increase participant’s knowledge about mental health, from 57.84% to 95.47%.
Preclinical Study of n-Hexane Extract of Kapul Fruit (Baccaurea macrocarpa) as an Anti-Inflammatory Rizki, Muhammad Ikhwan; Rahmatullah, Satrio Wibowo; Izma, Hayatun; Isnani, Nazhipah; Ramadhani , Syahrizal
BORNEO JOURNAL OF PHARMASCIENTECH Vol 9 No 2 (2025): Borneo Journal Of Pharmascientech
Publisher : Universitas Borneo Lestari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59053/bjp.v9i2.769

Abstract

Plants of the genus Baccaurea have long been traditionally used in folk medicine. Previous studies reported that the n-hexane extract of kapul fruit (Baccaurea macrocarpa) exhibited extreme antioxidant activity with an IC₅₀ value of 33.11 ppm. However, its potential anti-inflammatory activity has not been extensively investigated. The results demonstrated that all three doses of the n-hexane extract exhibited anti-inflammatory activity, as indicated by a reduction in edema volume. Among the tested doses, 600 mg/kg BW was the most effective ini decreasing edema volume. Statistical analysis using one-way ANOVA showed a significance value of 0.000, confirming a significant difference among the five treatment groups. Furthermore, post hoc analysis indicated that dose III did not differ significantly from the positive control but showed a significant difference compared to the negative control. Conversely, doses I and II exhibited significant differences when compared to both the positive and negative controls. These findings suggest that the n-hexane extract of B. macrocarpa fruit possesses anti-inflammatory activity, with the 600 mg/kg BW dose demonstrating the most potent effect, comparable to that of diclofenac sodium
Pelatihan Pembuatan Jamu Milenial Berbahan Pangan Lokal Kalimantan Daun Kalakai (Stenochlaena palustris) pada Ibu PKK Desa Sungai Besar Kabupaten Banjar: Training in Herbal Medicine Production from Kalakai Leaves to Prevent Stunting for PKK Mothers in Sungai Besar Village, Banjar Regency Wati, Helmina; Susiani, Eka Fitri; Effendi, Ibrahim Rully; Triyasmono, Liling; Nugroho, Agung; Rizki, Muhammad Ikhwan
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 9 No. 12 (2024): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v9i12.8608

Abstract

Stunting is defined as a chronic nutritional problem caused by a lack of adequate nutritional intake over an extended period. Iron is a vital component for cellular growth and development, as well as immune system function. The solution to overcoming iron deficiency is to consume highly nutritious foods, such as meat and green leafy vegetables. One such food is the kalakai plant. The Sungai Besar Village, Banjar Regency, is characterized by an abundance of natural resources that flourish in a wild state. Among these is the kalakai plant (Stenochlaena palustris (Burnm. F.) Bedd.), which is notable for its high iron content. The plant is employed solely as a vegetable. The objective of this community service activity is to enhance the competencies and understanding of PKK mothers in Sungai Besar Village about the diversification of processed kalakai plant products into millennial herbal medicine products. The methodology employed involved the preparation of the field, the counseling of the potential of kalakai leaves, and the training in the production of diversified kalakai leaves into millennial herbal medicine. The results and conclusions of this activity demonstrated an increase in knowledge of 85% and an 89% increase in the skills of PKK mothers in Sungai Besar Village in the diversification of kalakai leaves for the production of millennial herbal medicine derived from local Kalimantan plants.
Co-Authors Aghna Mafruha Zahrah, Aghna Mafruha Agung Nugroho Agustina, Ni Kadek Ayu Akbar, Nabila Hadiah Akhmad Nabil Akhmad Nabil, Akhmad Akhsanul Rahmatullah Alfandani, Gina Alifni Adha Bakti Amali, Dzikra Aulia Amalia Khairunnisa Amalia Khairunnisa Amalia Khairunnisa, Amalia Amalia Khirunnisa Ana Ulfah Anna Humaira Anna Khumaira Sari Anna Khumaira Sari Aprianto . Arnida Arnida Arnida Arnida Aulea Rahmawati Azhara, Desiya Damayanti Baharuddin Yusuf Baharudin Yusuf Barkinah, Tut Beny Rahmanto Beny Rahmanto Beny Rahmanto Buih, Putri Helena Junjung Devi Eka Pratama Dewi Kartika Dewi Sari Mulia Dewi, Anita Ratna Dewi, Vonny Khresna Difa Intannia Dyah Anggraeni Effendi, Ibrahim Rully Evi Mintowati Evi Mintowati, Evi Evi Mulyani Fadlilaturrahmah Fadlilaturrahmah, Fadlilaturrahmah Fadlillaturahmah Fadlillaturahmah Fajar Lestari Fajar Lestari Fajar Lestari Fariz, Abshar Fauzi, Rakhmat Gina Alfandani Hayatun Izma Helmina Wati Heri Budi Santoso Herningtyas Nautika Lingga Hilneser Simamora Humaidy, Mizan Izma, Hayatun Khalifah, Sefa Nur Khoerul Anwar Khoerul Anwar Khoerul Anwar Khumaira Sari, Anna Kirana, Rita Laura Sarwo Liling Triyasmono Lutfi Chabib, Lutfi Madani, Al Malahayati, Siti Maman Turjaman Maman Turjaman Ma’shumah Ma’shumah Metty Amperawati Mia Fitriana Mohammad Rizki Fadhil Pratama Muhammad Rusydi Taufik Mutia Mawaddah Naning Kisworo Utami Naning Kisworo Utami Nashrul Wathan Nazhipah Isnani Normaidah, Normaidah Nugroho, Agung Nur Mahdi Nurlely, Nurlely Okta Muthia Sari Oktavia Indrati, Oktavia Putra, Aditya Maulana Perdana Rabiatul Adawiyah Rahma, Salsabila Fadiya Rahmanto, Beny Rahmatullah, Satrio Wibowo Rahmawati, Aulea Ramadhan, Fery Ramadhani , Syahrizal Ratnapuri, Prima Happy Redanti, Hema Novita Rorry Adivrio Rizky Salamiah . Salamiah Salamiah Samsul Hadi Sandi, Dita Ayulia Dwi Saragih, Triando Hamonangan Sari , Anna Khumaira Sari, Fitri Novita Sholihin, Muhammad Aditya Silviana, Mega Siswadi Siswadi Sofia Suratno Suratno Surya Wahyudi Susiani, Eka Fitri Susy Andriani Sutomo Sutomo Sutomo Sutomo Syifa Auliani Syifa Auliani Titin Nor Tjitradi, Sukhito Yohanes Totok Wianto Valentina Meta Srikartika Wawan Halwany Wawan Halwany Wawan Halwany Wawan Halwany, Wawan Yuni Kartika