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Uji toksisitas menggunakan metode BSLT dan uji antibakteri Staphylococcus aureus dan Escherichia coli ekstrak dan fraksi batang karamunting (Rhodomyrtus tomentosa (Aiton) Hassk.) Siregar , Anisah; Ridwanto, Ridwanto; Daulay , Anny Sartika; Nasution , Haris Munandar
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 1 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i1.822

Abstract

Natural ingredients have been widely used in medicine, commonly referred to as "back to nature," meaning "kembali ke alam." Herbal medicine is recognized as one of the most practical and effective treatment modalities available. One medicinal plant is the stem of Rhodomyrtus tomentosa (Aiton) Hassk., which belongs to the Myrtaceae family and has the potential as an antibacterial remedy. To ensure traditional medicine fulfills its responsibility, scientific research, such as toxicity testing, is necessary. The Brine Shrimp Lethality Test (BSLT) is a preliminary test to assess the toxic effects of plants. This study aims to determine the secondary metabolite compounds, toxicity levels, and antibacterial activity of ethanol extract and n-hexane and ethyl acetate fractions of Rhodomyrtus tomentosa stem. The study includes characterization, phytochemical screening, and toxicity testing by observing the mortality rate of Artemia salina Leach larvae, expressed as LC50. Antibacterial testing against Staphylococcus aureus and Escherichia coli was conducted using the disc diffusion method. The results of phytochemical screening of simplicia powder, ethanol extract, and n-hexane and ethyl acetate fractions of Rhodomyrtus tomentosa stem tested positive for alkaloids and triterpenoids/steroids. Simplicia powder, ethanol extract, and the ethyl acetate fraction also contained secondary metabolites such as flavonoids, tannins, and saponins. The toxicity test results using probit analysis showed that the LC50 value of the extract was 97.6787 µg/mL, the LC50 value of the ethyl acetate fraction was 71.4331 µg/mL, and the LC50 value of the n-hexane fraction was 57.4910 µg/mL. Therefore, it can be concluded that the extract and the n-hexane and ethyl acetate fractions of Rhodomyrtus tomentosa stem are toxic. The antibacterial test results showed that the extract and the n-hexane and ethyl acetate fractions exhibited inhibitory activity against Staphylococcus aureus and Escherichia coli.
Uji toksisitas menggunakan metode BSLT dan uji antibakteri Staphylococcus aureus dan Escherichia coli ekstrak dan fraksi daun karamunting (Rhodomyrtus tomentosa (Aiton) Hassk.) Sagala , Vipi Zetiara; Ridwanto, Ridwanto; Daulay , Anny Sartika; Pulungan , Ainil Fithri
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 1 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i1.823

Abstract

Karamunting leaves are a Myrtaceae family plant used as an antioxidant, antimicrobial, antiviral, anticancer, and antibacterial. This research aimed to determine secondary metabolite compounds, toxicity effects, and antibacterial activity in extracts and fractions of karamunting (Rhodomyrtus tomentosa (Aiton) Hassk.) leaves. This research includes characteristics, phytochemical screening, a BSLT (Brine Shrimp Lethality Test) method toxicity test, and an antibacterial test using the paper disc method. Toxicity tests were carried out with concentrations of (100, 200, 300, 400, 500, 600, 700, 800, 900, 1000) µg/mL, and antibacterial tests against staphylococcus aureus and Escherichia coli were carried out with concentrations of 10, 20 and 30%. The ethanol extract and ethyl acetate fraction results contain alkaloids, flavonoids, triterpenoids or steroids, tannins, and saponins. And the n-hexane fraction contains triterpenoids or steroids. Toxicity test of ethanol extract, n-hexane fraction, and ethyl acetate of karamunting leaves (Rhodomyrtus tomentosa (Aiton) Hassk.) has a toxic effect on Artemia Salina Leach. In the ethanol extract, an LC50 value of 92.593 µg/mL was obtained, followed by the n-hexane fraction with an LC50 value of 153.3829 µg/mL. Then, the ethyl acetate fraction LC50 value was 75.9328 µg/mL. LC50 value. Based on the results obtained from the ethanol extract, the n-hexane and ethyl acetate fractions of caramunting leaves (Rhodomyrtus tomentosa (Aiton) Hassk.) were classified in the toxic category, and the antibacterial test of the ethanol extract, the n-hexane and ethyl acetate fractions of caramunting leaves (Rhodomyrtus tomentosa (Aiton) Hassk.) has antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria. The most potent inhibitory power was found in the ethyl acetate fraction with a concentration of 30% against staphylococcus aureus 16.1 mm and Escherichia coli 17.1 mm in the strong category.
Antibacterial Activity Test of Malacca Leaf Extract (Phyllanthus emblica) Against Staphylococcus aureus and Staphylococcus epidermidis Bacteria Ridwanto, Ridwanto; Aprilia, Hijjatun; Rani, Zulmai; Putri Aisyia Fauzi, Ziza; Kaban, Vera Estefania; Nasri, Nasri
Indonesian Journal of Pharmaceutical and Clinical Research Vol. 7 No. 1 (2024): Indonesian Journal of Pharmaceutical and Clinical Research
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/idjpcr.v7i1.17912

Abstract

Antibacterials are substances that can interfere with the growth or even kill bacteria and have the side effect of antibacterial resistance. One effort to reduce resistance is choosing to use natural bacterial ingredients. One plant that can potentially contain antibacterial activity is malacca leaves (Phyllanthus emblica). The contents of this plant include flavonoids, alkaloids, tannins, saponins, and steroids/triterpenoids. This research aimed to prove the antibacterial activity test of malacca leaf extract. The stages in this research included phytochemical screening and testing the antibacterial activity of ethanol extract from Malacca leaves for Staphylococcus aureus and Staphylococcus epidermidis bacteria using the diffusion method using paper discs. The characteristics of malacca leaf simplicia obtained were water content of 4.6%, water-soluble content of 28%, ethanol-soluble essence content of 23.33%, total ash content of 2.00%, and insoluble ash content of 0.5%. The results of phytochemical screening showed the presence of flavonoids, saponins, tannins, glycosides, and steroids/triterpenoids. The antibacterial test results of malacca leaf extract at concentrations of 500 mg/ml, 400 mg/ml, 300 mg/ml, and 200 mg/ml have an antibacterial effect against Staphylococcus aureus and Staphylococcus epidermidis, and there is no difference in the effectiveness of the inhibitor against gram-positive and negative grams.
Urgensi Pelaksanaan Corporate Social Responsibility Pada Perusahaan Di Indonesia Perspektif Ekonomi Islam: Sebuah Meta-Sintesis Ridwanto, Ridwanto; Misbach, Irwan; Siradjuddin, Siradjuddin
Jurnal Mirai Management Vol 8, No 3 (2023)
Publisher : STIE AMKOP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37531/mirai.v8i3.6217

Abstract

Corporate that is succeed in implementing CSR practices effectively can gain the public trust, improve the company’s image and reputation, and support the achievement of further development goals. In the context of Islamic Economics, CSR is not only considered as an additional social responsibility, but also as an obligation that must be in line with Islamic business ethics. Implementing CSR within an Islamic economic framework is an integral part of the company's commitment to providing a sustainable positive impact in society. This research uses a meta-synthesis method to investigate the implementation of CSR in companies in Indonesia from an Islamic economics perspective. Literature analysis shows that CSR provides certainty of welfare to employees, contributes to community empowerment, and ensures environmental sustainability. These findings provide depth insight into the importance of implementing CSR in achieving sustainable development goals and Islamic economic values in Indonesia.
Pemikiran Ekonomi Islam Masa Daulah Abbasiyah Ridwanto, Ridwanto; Siradjuddin, Siradjuddin
Journal of Economics and Business UBS Vol. 12 No. 5 (2023): Special Issue
Publisher : Cv. Syntax Corporation Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52644/joeb.v12i5.516

Abstract

Daulah Abbasiyah yang dipelopori oleh al-Abbas Ibnu Muthalib muncul setelah tergulingnya kekuasaan Daulah Bani Umayyah, kekuasaan Daulah Abbasiyah berlangsung dalam waktu yang amat panjang dimulai 132 (750 M) hingga 656 H (1258 M). Pemikiran ekonomi Islam dalam masa ini terpusat pada kebijakan keuangan publik, meskipun secara regulasi tatanan keuangan Negara belum terbentuk secara komperhensif. Sistem perbankan telah dilakukan seiring dengan bertambahnya jenis mata uang yang berlaku. Regulasi perbankan ini dijalankan oleh para ahli bankir yang disebut naqid, sarraf, dan jihbiz. Sementara, pendapatan Negara berasal dari pajak dan juga zakat. Untuk pengeluarannya, difokuskan pada urusan administrasi Negara, militer, pembangunan infrastruktur dan sektor ekonomi yang vital seperti industri, perdagangan dan pertanian. Pada Dinasri Abbasiyah juga diterapkan berbagai tradisi pada sektor industri dengan melakukan produksi massal beberapa komoditi seperti rempah-rempah, sutra dan keramik. Di sektor perdagangan berkembang dengan terbentuknya jalur perdagangan internasional. Di bidang pertanian, difokuskan pada pembangunan infrastruktur penunjang. Beberapa regulasi juga diterapkan oleh masing-masing pemimpin pada masanya dimulai dari kepemimpinan al-Mahdi hingga al-Mutawakkil
ANALISIS KINERJA KEUANGAN PEMERINTAH DAERAH KABUPATEN PIDIE Ridwanto, Ridwanto; Arjuniadi, Arjuniadi; Abdullah, Husaini
MAFEBIS: Jurnal Manajemen, Akuntansi dan Bisnis Vol 2, No 3 (2024): SEPTEMBER 2024
Publisher : Fakultas Ekonomi Universitas Jabal Ghafur, Sigli. Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47647/.v2i3.2449

Abstract

AbstrakPenelitian ini bertujuan untuk mengetahui Kinerja Keuangan Pemerintah Daerah Kabupaten Pidie. Jenis data yang digunakan adalah kuantitatif, dimana berupa data sekunder Laporan Realisasi Anggaran Pendapatan Belanja Daerah penelitian ini menggunakan metode penelitian deskriptif kuantitatif. Hasil penelitian menunjukkan kinerja keuangan Pemerintah Kabupaten Pidie dilihat dari Rasio Derajat Desentralisasi Fiskal rata-rata 10,94% dapat disimpulkan belum mampu dalam membiayai pembangunan daerah. Dilihat dari rasio kemandirian daerah rata-ratanya 17,51% dimana masih sangat rendah menggambarkan kemampuan keuangan daerah dalam membiayai pelaksanaan pemerintahan dan pembangunan daerah masih tergantung dari bantuan pemerintah pusat. Dari Rasio Efektivitas PAD rata-rata 84,48% dikategorikan tidak efektif, ini dapat dikatakan Pemerintah memiliki kinerja kurang baik dalam merealisasikan PAD yang telah direncanakan. Dilihat dari Rasio Efisiensi rata-rata 100,13% dikategorikan tidak efisien ini dapat dikatakan kinerja Pemerintah masih buruk karena tidak dapat menekan jumlah belanja daerahnya, dan dari rasio keserasian, rata-rata belanja operasi lebih besar dibandingkan dengan belanja modal yaitu 56,36% berbanding 12,75%, berarti sebagian besar jumlah belanja daerah untuk pembiayaan belanja pegawai, gaji PNS dan lain-lain, pemerintah belum sepenuhnya memperhatikan pembangunan daerah.Kata kunci: Kinerja Keuangan, Rasio Desentralisasi, Rasio kemandirian, Rasio Efektifitas Rasio Efisiensi, Rasio Keserasian
Uji Aktivitas Antioksidan Ekstrak dan Fraksinasi Daun Tekelan (Chromolaena odorata (L.) R.M.King & H.Rob) Serta Uji Antibakteri Terhadap Staphylococcus aureus dan Escherichia coli Parapat , Anjarlit Romiyuliana Br; Ridwanto, Ridwanto; Yuniarti , Rafita; Rani , Zulmai
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 4 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i4.1059

Abstract

Background: Tekelan leaf (Chromolaena odorata (L.) R.M.King & H.Rob) is one of Indonesia's natural resources rich in bioactive compounds. Its traditional use for treating wounds, sore throats, diarrhea, and various other ailments is suspected to be linked to its antioxidant and antibacterial content. Objective: This research aimed to analyze the antioxidant activity of the ethanol extract, n-hexane fraction, and ethyl acetate fraction of tekelan leaves, and to test their antibacterial activity against Staphylococcus aureus and Escherichia coli. Method: The study used an experimental method. Antioxidant activity was tested using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method with a UV-Vis spectrophotometer, and the results were expressed as IC₅₀ values. The antibacterial test against S. aureus and E. coli was conducted using the disc diffusion method, and the inhibitory power was measured based on the inhibition zone diameter (mm). Results: The antioxidant test results showed that the ethanol extract and ethyl acetate fraction had strong activity with IC₅₀ values of 98.26 ppm and 75.43 ppm, respectively, while the n-hexane fraction showed weak activity (IC₅₀ 110.54 ppm). In the antibacterial test at a 40% concentration, the ethanol extract, n-hexane fraction, and ethyl acetate fraction inhibited the growth of both bacteria with strong to very strong categories. The highest average inhibition zones against E. coli and S. aureus were 19.03 mm and 21.36 mm (ethanol extract), 10.56 mm and 12.13 mm (n-hexane fraction), and 13.60 mm and 15.40 mm (ethyl acetate fraction), respectively. This activity was still lower than the positive control chloramphenicol, which produced inhibition zones of 28.23 mm (E. coli) and 27.43 mm (S. aureus). Conclusion: It was concluded that the extract and ethyl acetate fraction of tekelan leaves have strong antioxidant activity, while the n-hexane fraction is classified as weak. All samples (extract, n-hexane fraction, and ethyl acetate fraction) showed strong antibacterial activity against E. coli and S. aureus, although they have not matched the effectiveness of chloramphenicol.
Uji Aktivitas Antioksidan dan Antibakteri Ekstrak dan Hasil Fraksinasi Daun Bandotan (Ageratum conyzoides L.) Terhadap Bakteri Escherichia coli Dan Staphylococcus aureus Harahap, Sumiyarni; Ridwanto, Ridwanto; Pulungan, Ainil Fithri; Rani, Zulmai
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 4 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i4.1060

Abstract

Bandotan (Ageratum conyzoides L.) is a plant distributed worldwide, especially in tropical and subtropical regions. Bandotan can be used as a traditional medicine because it contains beneficial phytochemical compounds such as terpenoids, alkaloids, essential oils, saponins, and phenolics that play a role in inhibiting bacterial growth and serving as natural antioxidants. The objective of this research was to determine the antioxidant and antibacterial activity of the extract, n-hexane fraction, and ethyl acetate fraction from bandotan leaves. This research used an experimental method. It included antioxidant testing of the ethanol extract fractions of bandotan leaves (Ageratum conyzoides L.) using the DPPH method and antibacterial testing using Escherichia coli and Staphylococcus aureus bacteria. The phytochemical screening results showed that bandotan leaves contain alkaloids, saponins, tannins, flavonoids, and glycosides. The antioxidant testing for DPPH radical scavenging revealed that the bandotan leaf extract had an IC50 value of 499.3633 μg/ml, the n-hexane fraction had an IC50 value of 945.7837 μg/ml, and the ethyl acetate fraction had an IC50 value of 382.9216 μg/ml, all categorized as very weak antioxidants. Antibacterial testing indicated that the extract, n-hexane fraction, and ethyl acetate fraction of bandotan (Ageratum conyzoides L.) exhibited antibacterial activity, inhibiting the growth of Escherichia coli and Staphylococcus aureus, with strong activity at the highest concentration of 40% and moderate activity at the lowest concentration of 10%. In conclusion, bandotan leaves have very weak antioxidant activity due to the low content of flavonoids and strong antibacterial activity at 40% concentration and weak activity at 10% concentration.
Antioxidant Activity Test of Barangan Banana Hump’s Ethanol Extract (Musa Paradisiaca (L.)) with DPPH (1,1 Diphenyl-2-Picrylhydrazyl) Method Nurmazela, Virda; Ridwanto, Ridwanto; Rani, Zulmai
International Journal of Science, Technology & Management Vol. 3 No. 5 (2022): September 2022
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v3i5.610

Abstract

This study aims to determine the secondary metabolites contained in Barangan banana weevil (Musa paradisiaca (L.)) and antioxidant activity based on the IC50 value. The stages of this research include processing plant materials, making ethanol extracts, characterization examinations, phytochemical screening, and testing the antioxidant activity of Barangan banana weevil. The ethanol extract of Barangan banana weevil was processed by the maceration method using 96% ethanol, and the antioxidant activity was tested using the DPPH method. The results showed that the ethanol extract of Barangan banana weevil contained chemical compounds such as alkaloids, glycosides, flavonoids, saponins, tannins, and triterpenoids. The determination of the antioxidant activity of Barangan banana weevil obtained an IC50 value of 180.8 µg/mL. This means that the ethanol extract of Barangan banana weevil contains secondary metabolites and has the potential as an antioxidant with a weak category.
Utilization Of Chitosan As A Natural Preservative Against Catfish Nurhayati, Nurhayati; Ridwanto, Ridwanto; Sartika Daulay, Anny; Andi Syahputra, Ricky; Rani, Zulmai
International Journal of Science, Technology & Management Vol. 3 No. 5 (2022): September 2022
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v3i5.614

Abstract

Shrimp in Indonesia are generally exported abroad after removing the head, tail, and skin. One alternative to the use of shrimp shell waste that has high economic value is processing shrimp shells into chitosan. The purpose of this study was to determine the shelf life of catfish using chitosan and to determine the optimal concentration of chitosan in catfish preservation. Chitosan applied to catfish showed that in the addition of a 0% chitosan solution, it had a shelf life of up to day 2 for 48 hours for a 0.5 solution; 1; 1.5 and 2% shelf life up to day 5 or for 120 hours. The most optimal concentration of chitosan for use in catfish is a 2% chitosan solution, and the total plate number (ALT) test meets the requirements of the SNI standard so that it can still be consumed.
Co-Authors Abdullah, Husaini Abdullah, Muhammad Wahyuddin Adawiyah, Marissa Tasya Adyani Maulinda Afrizani, Afrizani Agus Priyadi Akbar, Windi Hari Al Hudawi, Muhammad Al Qodri, Muhammad Alviana, Liya Amin Nasution, Muhammad Amir, Annisa Fadilah Andi Syahputra, Ricky Ani Sutiani Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Aprilia, Hijjatun Aprilia, Medhy Ardhita Arifin, Khairil Fikri Arjuniadi, Arjuniadi Audry Pratiwi Audry Pratiwi Azhar, Gustika Azri, Atika Azzahra, Fadya Cici Andriani Claudy Friesta Melanie Dalimunthe, Gabena Indriyani Damanik, Sumayyah Daniel Happy Putra Daulay , Anny Sartika Daulay, Ani Sartika Daulay, Anny S. Denni Bahari Dikki Miswanda Dina Suciati Saragih Dison Silalahi, Alistraja Elma Natasya Fadhilah M, Ihsan Fadhlah Al-Uyun Nasution Fajri, Fajrilda Aqilah Fathur Rahman Fathur Rahman Fatur Rahman Fithri Pulungan, Ainil FRANSISKA, EVA Gabena Indrayani Dalimunthe Hafizha, Putri Harahap, Ade Try Atwinda Harahap, Siti Salimah Harahap, Sumiyarni Haris Munandar Nasution Harun , Fathur Rahman Harun , Fatur Rahman Harun, Fathur Rahman Hasanah, Qori Hasibuan , Nabila Hasim Hasibuan, Sarinah Hasibuan, Supia Indah Hazizah, Mautia Hermawan, Fiqri Humairah, Dwina Angelina Imelda Natasia Br S Meliala Irvan Andreansyah Irwan Misbach Isnaria, Vita Khairunnisa Khairunnisa Lastri Afni Lince Bulutoding Lubis, Minda Sari M. Naufal Rifqi Maghfirah , Dini Makhfirah Malahayati Malahayati Mambang, D Elysa Putri Manik, Umi Chairani Manurung, Aisyah Baddriah Maryanti Yuza Melinda, Reni Meliyana Meliyana Merani Phaustina Lumban Gaol Mirna Mirna Muchlis, Syaiful Muhammad Amin Nasution Muhammad Amin Nasution Muhammad Wahyudi Munthe, Ariska Munthe, Herlina Nada, Dina Qatrun Nadila Indra Sepvina Nasri Nasri, Nasri Nasution, Kartika Zsaskia Nasution, M. Amin Nasution, Shamila Sufi Aulia Nazirah, Nazirah Ningtias, Anggitha Nst, Haris Munandar Nur Ainun Nuraida Nuraida Nurhayati Nurhayati Nurliansyah, Nurliansyah Nurmazela, Virda Parapat , Anjarlit Romiyuliana Br Pitaloka, Syifa Amilia Pulungan, Ainil Fitri Puteri, Cut Intan Annisa Putri Khairani Putri Theresia Harianja Putriyana Putriyana Qisti Pristiwani Rahayu , Yayuk Putri Rahayu Rahayu Rahma Dona, Rahma Rahmadani , Adevika Rahmadani Rahmadani Rahmah, Ruhiya Rahmasari, Siti Rahmayanti, Putri Rambe, Robiatun Reza Irma Ricky Andi Syahputra Rika Yuliana, Rika Riski Mulyani, Riski Risma Fauziah Pasaribu Rizki, Dewi Rizki, Rahmad Rosaldi, Hikmah Rossa, Aliffa S Sumarlin Sagala , Vipi Zetiara Salwa, Shakhila Saragih, Alkausar Sari, Farida Sartika Daulay, Anny Sasnita, Merida Selfia Lestary Shilvia, Fatin Sinaga, Novita Yulianti Siradjuddin, Siradjuddin Siregar , Anisah Siregar, Muhammad Alfariz Baihaqi Sitompul, M. Saleh Sitorus, Dara Indah Sri Wahyuni Syahfitri , Adelya Syahputra, Ricky Andi Syalsabila Putri Syarifah Aulia Tamara Army Tambupolon, Sanya Tanika Trizaldi, Asep Utama, Fazrul Amin Vera Estefania Kaban Wijiyaningsih, Dinda Yanti, Rahma Yayuk Putri Rahayu Yulia Nanda Putri Yuniarti , Rafita Yuniarti, Rafita Ziza Putri Aisyia Fauzi Zulmai Rani