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Penetapan kadar flavonoid total ekstrak kayu raru (Cotylelobium lanceolatum Craib) berdasarkan perbedaan konsentrasi etanol dengan metode spektrofotometri Uv-Vis Elma Natasya; Anny Sartika Daulay; Ridwanto Ridwanto; Yayuk Putri Rahayu
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.305

Abstract

The utilization of traditional medicinal plants for healing is the oldest form of medicine in the world. One of the plants that have many benefits and contain secondary metabolite compounds is raru bark (Cotylelobium lanceolatum Craib), used as a traditional medicine for diseases such as malaria, diarrhea, and diabetes. This study aims to determine what groups of compounds are contained in raru bark extract and the value of total flavonoid levels contained in raru bark extract in differences in ethanol concentrations (96%, 70%, 50%). The stages of this research include processing plant materials, making ethanol extracts of raru bark extract, characterization examination, phytochemical screening, and determining the total flavonoid content of raru bark ethanol extract. The section of raru bark was made by maceration method using 96%, 70%, and 50% ethanol. Then, the extract obtained was concentrated with a rotary evaporator, and the determination of total flavonoid content was carried out based on differences in ethanol concentration using the UV-Vis spectrophotometric method. The results showed that the ethanol extract of raru bark contained alkaloids, flavonoids, saponins, tannins, and steroid compounds. Determination of total flavonoid content by determining the maximum wavelength of quinine and calculating entire flavonoid content by UV-Vis spectrophotometric method. The results of the decision of complete flavonoid content of raru bark ethanol extract at 96% concentration are 1.9915 ± 0.0139 mg QE/g, 70% is 1.12492 ± 0.01532 mg QE/g, and for 50% is 0.95197 ± 0.01516 mg QE/g. So, the best ethanol concentration to produce flavonoid levels is 96%, which is higher than other ethanol concentrations.
Penetapan kadar fenolik total ekstrak kayu raru (Cotylelobium lanceolatum Craib) berdasarkan perbedaan konsentrasi etanol dengan metode spektrofotometri UV-Vis Lastri Afni; Anny Sartika Daulay; Ridwanto Ridwanto; Yayuk Putri Rahayu
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.306

Abstract

Many Indonesian people use or use plants as traditional medicine; one plant that has many benefits is raru bark (Cotylelobium lanceolatum Craib), which is used as a traditional medicine in various diseases such as diarrhea, malaria, and diabetes. This study aims to find out what classes of compounds are found in raru bark extract and to determine the value of total phenolic levels contained in raru bark extract in various concentrations (96%, 70%, 50%). The stages of this research include processing plant materials, making raru bark ethanol extract, characterization examination, phytochemical screening, and determining total phenolic levels of raru bark extract based on differences in ethanol concentration by UV-Vis spectrophotometry method. The results showed that raru bark ethanol extract contains compounds, flavonoids, tannins, saponins, steroids, and phenolics. Determination of total phenolic content by determining the maximum wavelength of gallic acid and calculation of entire phenolic content by UV-Vis spectrophotometry method. Results of selecting the whole phenolic content of raru bark ethanol extract at a concentration of 96%; 28,6544±0,1548 mgGAE/g, 70%; 23,9682±0,1270 mgGAE/g, 50%; 20,5825±0,1993 mgGAE/g, then the phenolic content that best produces total phenolic levels is raru bark ethanol extract (Cotylelobium lanceolatum Craib) concentration of 96%.
Isolasi Dan Identifikasi Kitosan Dari Cangkang Kerang Bulu (Anadara antiquata) Dewi Rizki; Ridwanto; Zulmai Rani
Media Farmasi Vol 19 No 2 (2023): Media Farmasi
Publisher : Jurusan Farmasi Poltekkes kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/mf.v19i2.174

Abstract

Chitosan is a polysaccharide formed via deacetylation of chitin. In general, chitosan is made from waste products from the fishing industry, such as shrimp, crabs, shells of feather clams, snails, and so on. The chitosan comes from the head, skin, and carapace. The development of chitosan applications has great potential because the production of shrimp, crab, and feather clam shells continues to increase. Chitosan is a bioactive substance with use in the fishing industry. This research aims to isolate and characterize chitosan from feather clam shells (Anadara antiquata) to convert feather clam shells into chitin and deacetylate it into chitosan then to determine the functional groups and characteristics of chitosan using Fourier infrared (FTIR). The isolation methods used were deproteination using 4% NaOH solvent, demineralization with a 1:5 ratio of 2N HCl solvent, depigmentation with a 1:10 ratio of 4% NaOCl solvent and deacetylation with a 1:20 ratio of 50% NaOH solvent. and chitosan characterization. According to the study's findings, the degree of deacetylation obtained in the form of a white powder was 75%, odorless fine powder. Chitosan dissolves in acetic acid, according to the solubility test. The obtained water content was 3.81% and the ash content obtained was 0.7%. Feather clam shell waste is declared to be well isolated and meets the requirements by SNI with a degree of deacetylation value of 75%. Kitosan merupakan polisakarida dari deasetilasi kitin; biasanya dibuat dari limbah produk industri perikanan seperti bekicot, kepiting, udang, bulu kerang, dan cangkang kerang. Kitosan berasal dari kulit, karapas, dan bagian kepala. Karena produksi udang, kepiting, dan cangkang kerang bulu yang terus meningkat, pengembangan aplikasi kitosan sangat potensial. Kitosan merupakan bahan bioaktif memiliki manfaat untuk industri perikanan. Tujuan studi ini adalah untuk mengidentifikasi, menggambarkan kitosan yang ditemukan dalam cangkang kerang bulu (Anadara antiquata) agar merubah cangkang kerang bulu menjadi kitin dan di deasetilasi menjadi kitosan kemudian untuk mengetahui gugus fungsi dan karakteristik kitosan menggunakan fourier infrared (FTIR). Metode isolasi yang dilakukan yaitu  deproteinasi menggunakan pelarut NaOH 4%, demineralisasi menggunakan pelarut HCl 2N dengan perbandingan 1:5, depigmentasi menggunakan pelarut NaOCl 4% (1:10), deasetilasi dengan pelarut NaOH 50% (1:20) dan karakterisasi kitosan. Hasil penelitian menunjukkan derajat deasetilasi yang di peroleh yaitu 75% berbentuk serbuk halus berwarna putih dan  tidak berbau. Menurut uji kelarutan, kitosan larut dalam asam asetat. Kadar air yang diperoleh 3,81% dan kadar abu yang diperoleh 0,7%. Limbah cangkang kerang bulu dinyatakan terisolasi dengan baik dan telah memenuhi syarat sesuai dengan SNI dengan nilai derajat deasetilasi sebesar 75%.
Isolasi Dan Identifikasi Kitosan Dari Cangkang Kerang Bulu (Anadara antiquata) Dewi Rizki; Ridwanto; Zulmai Rani
Media Farmasi Vol 19 No 2 (2023): Media Farmasi
Publisher : Jurusan Farmasi Poltekkes kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/mf.v19i2.174

Abstract

Chitosan is a polysaccharide formed via deacetylation of chitin. In general, chitosan is made from waste products from the fishing industry, such as shrimp, crabs, shells of feather clams, snails, and so on. The chitosan comes from the head, skin, and carapace. The development of chitosan applications has great potential because the production of shrimp, crab, and feather clam shells continues to increase. Chitosan is a bioactive substance with use in the fishing industry. This research aims to isolate and characterize chitosan from feather clam shells (Anadara antiquata) to convert feather clam shells into chitin and deacetylate it into chitosan then to determine the functional groups and characteristics of chitosan using Fourier infrared (FTIR). The isolation methods used were deproteination using 4% NaOH solvent, demineralization with a 1:5 ratio of 2N HCl solvent, depigmentation with a 1:10 ratio of 4% NaOCl solvent and deacetylation with a 1:20 ratio of 50% NaOH solvent. and chitosan characterization. According to the study's findings, the degree of deacetylation obtained in the form of a white powder was 75%, odorless fine powder. Chitosan dissolves in acetic acid, according to the solubility test. The obtained water content was 3.81% and the ash content obtained was 0.7%. Feather clam shell waste is declared to be well isolated and meets the requirements by SNI with a degree of deacetylation value of 75%. Kitosan merupakan polisakarida dari deasetilasi kitin; biasanya dibuat dari limbah produk industri perikanan seperti bekicot, kepiting, udang, bulu kerang, dan cangkang kerang. Kitosan berasal dari kulit, karapas, dan bagian kepala. Karena produksi udang, kepiting, dan cangkang kerang bulu yang terus meningkat, pengembangan aplikasi kitosan sangat potensial. Kitosan merupakan bahan bioaktif memiliki manfaat untuk industri perikanan. Tujuan studi ini adalah untuk mengidentifikasi, menggambarkan kitosan yang ditemukan dalam cangkang kerang bulu (Anadara antiquata) agar merubah cangkang kerang bulu menjadi kitin dan di deasetilasi menjadi kitosan kemudian untuk mengetahui gugus fungsi dan karakteristik kitosan menggunakan fourier infrared (FTIR). Metode isolasi yang dilakukan yaitu  deproteinasi menggunakan pelarut NaOH 4%, demineralisasi menggunakan pelarut HCl 2N dengan perbandingan 1:5, depigmentasi menggunakan pelarut NaOCl 4% (1:10), deasetilasi dengan pelarut NaOH 50% (1:20) dan karakterisasi kitosan. Hasil penelitian menunjukkan derajat deasetilasi yang di peroleh yaitu 75% berbentuk serbuk halus berwarna putih dan  tidak berbau. Menurut uji kelarutan, kitosan larut dalam asam asetat. Kadar air yang diperoleh 3,81% dan kadar abu yang diperoleh 0,7%. Limbah cangkang kerang bulu dinyatakan terisolasi dengan baik dan telah memenuhi syarat sesuai dengan SNI dengan nilai derajat deasetilasi sebesar 75%.
UJI TOKSISITAS KITOSAN CANGKANG KERANG TAHU (Meretrix meretrix L.) DENGAN METODE BRINE SHRIMP LETHALITY TEST (BSLT) Audry Pratiwi; Ridwanto Ridwanto
Jurnal Farmasi Klinik dan Sains Vol 2, No 2 (2022): Jurnal Farmasi Klinik dan Sains
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas Muhammadiyah Gombong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26753/jfks.v2i2.984

Abstract

Kerang tahu (Meretrix meretrix L) merupakan salah satu sumber daya perikanan Indonesia. Tujuan dari penelitian ini adalah Untuk mengetahui tingkat toksisitas kitosan dari cangkang Kerang tahu dengan melihat nilai LC50 yang diujikan pada metode BSLT. Penelitian ini meliputi Isolasi kitin dan kitosan: Deproteinasi, Demineralisasi, Depigmentasi dan deasetilasi kitin menjadi kitosan, karakterisasi kitosan, FTIR, dan Uji Toksisitas kitosan dengan menggunakan metode BSLT untuk melihat jumlah kematian larva Artemia salina L diperoleh data (LC50). Hasil Penelitian menunjukkan bahwa Kitosan Cangkang Kerang tahu memiliki LC50 4383.287934 μg/ml. Hasil tersebut menunjukkan bahwa kitosan cangkang Kerang tahu bersifat tidak toksik (LC50> 1000 μg/ml) pada uji BSLT.
OPTIMASI PEMBUATAN TEPUNG TALAS TERMODIFIKASI (Modified Taro Flour) DENGAN FERMENTASI Lactobacillus casei BERDASARKAN KADAR PROTEIN Imelda Natasia Br S Meliala; Anny Sartika Daulay; Ridwanto Ridwanto; Haris Munandar Nasution
Jurnal Farmasi Klinik dan Sains Vol 3, No 1 (2023): Jurnal Farmasi Klinik dan Sains
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas Muhammadiyah Gombong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26753/jfks.v3i1.1110

Abstract

Ketergantungan penggunaan tepung terigu dapat dikurangi dengan menggunakan sumber karbohidrat lainnya seperti talas yang merupakan pangan lokal. Lactobacillus casei adalah salah satu spesies Bakteri Asam Laktat yang dapat menggunakan selobiosa sebagai sumber nutrisinya. Diperkirakan Lactobacillus casei dapat mendegresi sel umbi talas agar meningkatkan kadar protein talas. Diantara faktor yang dapat mempengaruhi kondisi fermentasi untuk menghasilkan tepung termodifikasi yang optimal adalah waktu fermentasi. Penelitian ini bertujuan untuk mengetahui pengaruh optimasi fermentasi umbi talas terhadap organoeleptis dan kadar protein pada tepung talas termodifikasi. Penelitian ini menggunakan metode eksperimental dengan dua optimasi waktu fermentasi yaitu fermentasi 48 jam dan fermentasi 72 jam dan uji karakterisasi yaitu uji organoleptis, uji kadar air, uji kadar abu dan uji kadar protein. Hasil penelitian ini menunjukkan organoleptis tepung mocaf, tepung talas, tepung talas termodifikasi berwarna coklat dan putih kecoklatan, bau sedikit asam dan tekstur yang halus. Kadar protein mocaf yaitu 2,17, tepung talas 2,33%, tepung talas termodifikasi 48 jam 3,16%, tepung talas termodifikasi 72 jam 3,53%. Kadar air tepung mocaf 11,31%, tepung talas 8,67%, tepung talas termodifikasi sebesar 10,81%. Kadar abu tepung mocaf 1,13%, tepung talas 2,73%, tepung talas termodifikasi 1,82%. Hasil yang diperoleh menunjukkan bahwa  fermentasi 72 jam merupakan hasil yang optimal.
Penentuan Kadar Meloksikam Sediaan Tablet Dari Berbagai Apotek Yang Terdapat Di Rantau Prapat Secara Spektrofotometri – UV Risma Fauziah Pasaribu; Anny Sartika Daulay; Ridwanto Ridwanto; Rafita Yuniarti
USADA NUSANTARA : Jurnal Kesehatan Tradisional Vol. 1 No. 2 (2023): Juli: USADA NUSANTARA
Publisher : Institut Nalanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47861/usd.v1i2.329

Abstract

Meloxicam is a non-steroidal anti-inflammatory drug that can relieve arthritis symptoms, such as inflammation, swelling, and muscle stiffness and pain, by acting as an inhibitor of enzymes that produce prostaglandins. In the Indonesian Pharmacopoeia Edition V (2014), the determination of sugar tablet content was carried out using high performance liquid chromatography. This method is expensive compared to the spectrophotometric method. The purpose of this research is to find an alternative method that is simple and inexpensive but provides good accuracy. This research was descriptive in nature using spectrophotometry-UV method, with 0.1 N NaOH Methanol as a solvent. The samples used in this study were 4 generic meloxicam tablets. The test parameters of this research included the preparation of Standard Mother Solution I (LIB I). Maximum wavelength, Linearity Protection of the Calibration Curve and Determination of the Concentration of Meloxicam in Tablet Preparations. From the results of testing the samples, generic meloxicam tablets were taken from PT. (Dankos pharma) 102.47 ± 0.9868%; generic meloxicam tablets (PT. Etercon Pharma) 98.05 ± 1.0930%; generic meloxicam tablets (PT. Mahakam Beta Farma) 99.79 ± 1.046%; generic tablets (Triman) 101.21 ± 2.4345%. All the tablets specified meet the general strength requirements, that is, the active ingredient content is not less than 90% and not more than 110% of the amount stated on the label.
Uji Aktivitas Antioksidan Teh Herbal Daun Melinjo (Gnetum Gnemon L.) Dengan Menggunakan Spektrofotometri Visibel Syarifah Aulia; Rafita Yuniarti; Gabena Indrayani Dalimunthe; Ridwanto Ridwanto
USADA NUSANTARA : Jurnal Kesehatan Tradisional Vol. 1 No. 2 (2023): Juli: USADA NUSANTARA
Publisher : Institut Nalanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47861/usd.v1i2.330

Abstract

Antioxidants are compounds that can counteract / reduce the negative effects of free radicals that cause damage to the body. Natural antioxidants are used as an alternative in an effort to minimize the use of synthetic antioxidants which can cause carcinogens. Utilization of melinjo leaves as herbal tea is one effort to use natural antioxidants to minimize the use of synthetic antioxidants which can cause carcinogens. The purpose of this study was to determine the content of secondary metabolites contained in melinjo leaf tea, the characteristics of melinjo leaf tea and the antioxidant activity of melinjo leaf tea. This research was conducted by making melinjo leaf herbal tea through a drying process in a drying cabinet at 40oC, phytochemical screening, examining the characteristics of the tea, and testing the antioxidant activity. The results of the Phytochemical screening examination showed that melinjo leaf herbal tea contains alkaloids, flavonoids, saponins, tannins, steroids/triterpenoids. As well as having the characteristics of water content 5.6866%, water extract 36.9740%, total ash content 5.7658%, water soluble ash content to total ash 7.7066, acid insoluble ash content 0.54%. While the antioxidant activity test of melinjo leaf herbal tea showed very weak antioxidant activity with an IC50 value of 501.7622 ppm
PERBANDINGAN KADAR PROTEIN PADA KUNING DAN PUTIH TELUR BEBEK REBUS MENGGUNAKAN METODE KJELDAHL DAN SPEKTROFOTOMETRI VISIBLE M. Naufal Rifqi; Daulay, Anny Sartika; Ridwanto; Rafita Yuniarti
FARMASAINKES: JURNAL FARMASI, SAINS, dan KESEHATAN Vol. 4 No. 1 (2024): FARMASAINKES: JURNAL FARMASI, SAINS dan KESEHATAN
Publisher : Fakultas Farmasi Universitas Muslim Nusantara Al Washliyah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32696/farmasainkes.v4i1.3350

Abstract

Pendahuluan: Salah satu telur unggas yang banyak dikonsumsi masyarakat adalah telur bebek Telur merupakan sumber protein hewani dan lauk pauk yang mudah didapat, murah, dan penuh nutrisi. Telur memberikan nutrisi lengkap yang diperlukan untuk pertumbuhan sel. Telur juga menyediakan semua asam amino penting bagi manusia, yang membuatnya menjadi sumber protein berkualitas tinggi. Tujuan: Tujuan Penelitian ini adalah untuk mengetahui dan membandingan kadar protein dari kuning dan putih telur bebek rebus menggunakan metode kjeldahl dan spektrofotometri visible. Metode: Metode penelitian yang digunakan untuk mengukur kadar protein kuning dan putih telur bebek rebus menggunakan metode Kjeldahl dan spektrofotometri visible. Metode Kjeldahl meliputi destruksi, destilasi, dan titrasi, sedangkan metode spektrofotometri tampak meliputi pembuatan larutan biuret, penentuan panjang gelombang maksimum, pembuatan kurva standar, dan penentuan kandungan protein sampel. Hasil: Hasil penelitian didapat kadar protein pada telur rebus dengan metode kjeldahl yaitu kuning telur bebek rebus sebesar 9,5363% dan putih telur bebek rebus sebesar 11,6497%. Dengan metode spektrofotometri visible yaitu kuning telur bebek rebus sebesar 8,4794% dan putih telur bebek rebus sebesar 8,64375%. Terdapat perbedaan nyata kadar protein antara metode kjeldahl dengan spektrofotometri visible dengan hasil SPSS nilai Sig. 0,000 < 0,05.
Uji Sitotoksisitas Ekstrak Etanol Daun Bayam Duri (Amaranthus spinosus L.) dengan Metode Brine Shrimp Lethality Test (BSLT) Fauzi, Ziza Putri Aisyia; Ridwanto, Ridwanto; Rani, Zulmai; Arifin, Khairil Fikri
Journal of Pharmaceutical and Health Research Vol 5 No 2 (2024): June 2024
Publisher : Forum Kerjasama Pendidikan Tinggi (FKPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47065/jharma.v5i2.5398

Abstract

Free radicals can cause chronic disease, it takes years for the disease to become real or accumulative. One possibility that can occur as a result of the work of free radicals such as cancer. Cancer is one of the non-communicable diseases that is a public health problem, both in the world and in Indonesia. One of the plants that has the potential as an anticancer is Amaranthus spinosus L. The results showed that thorn spinach leaves contain strong antioxidants. This study aims to determine the class of secondary metabolites contained in the ethanolic extract of thorn spinach leaves and the cytotoxicity of the ethanolic extract of A.spinosus by looking at the LC50 value which was tested using the Brine Shrimp Lethality Test (BSLT) method. This research includes phytochemical screening of A.spinosus extract and Brine Shrimp Lethality Test (BSLT) method by looking at the number of larvae mortality of Artemia salina leach (LC50). The results of the phytochemical screening test showed that A.spinosus were positive for flavonoids, alkaloids, saponins, tannins, steroids and glycosides. The results of the cytotoxicity test using probit analysis showed an LC50 value of 269.15 g/mL, so it can be concluded that the ethanolic extract of A.spinosus is toxic and has potential as an anticancer.
Co-Authors Abdullah, Husaini Abdullah, Muhammad Wahyuddin Adawiyah, Marissa Tasya Adyani Maulinda Afrizani, Afrizani Agus Priyadi Akbar, Windi Hari Al Hudawi, Muhammad Al Qodri, Muhammad Alviana, Liya Amin Nasution, Muhammad Amir, Annisa Fadilah Ani Sutiani Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Aprilia, Hijjatun Aprilia, Medhy Ardhita Arifin, Khairil Fikri Arjuniadi, Arjuniadi Asep Trizaldi Audry Pratiwi Audry Pratiwi Azhar, Gustika Azri, Atika Azzahra, Fadya Cici Andriani Claudy Friesta Melanie Dalimunthe, Gabena Indriyani Damanik, Sumayyah Daniel Happy Putra Daulay , Anny Sartika Daulay, Ani Sartika Daulay, Anny S. Denni Bahari Dikki Miswanda Dina Suciati Saragih Dison Silalahi, Alistraja Elma Natasya Eva Fransiska Fadhilah M, Ihsan Fadhlah Al-Uyun Nasution Fajri, Fajrilda Aqilah Fathur Rahman Fathur Rahman Fatur Rahman Fithri Pulungan, Ainil Gabena Indrayani Dalimunthe Hafizha, Putri Harahap, Ade Try Atwinda Harahap, Siti Salimah Harahap, Sumiyarni Haris Munandar Nasution Harun , Fatur Rahman Harun, Fathur Rahman Hasanah, Qori Hasibuan , Nabila Hasim Hasibuan, Sarinah Hasibuan, Supia Indah Hazizah, Mautia Hermawan, Fiqri Humairah, Dwina Angelina Imelda Natasia Br S Meliala Irvan Andreansyah Irwan Misbach Isnaria, Vita Khairunnisa Khairunnisa Lastri Afni Lince Bulutoding Lubis, Minda Sari M. Naufal Rifqi Maghfirah , Dini Makhfirah Mambang, D Elysa Putri Manik, Umi Chairani Manurung, Aisyah Baddriah Maryanti Yuza Melinda, Reni Meliyana Meliyana Merani Phaustina Lumban Gaol Mirna Mirna Muchlis, Syaiful Muhammad Amin Nasution Muhammad Amin Nasution Muhammad Wahyudi Munthe, Ariska Munthe, Herlina Nada, Dina Qatrun Nadila Indra Sepvina Nasri Nasri, Nasri Nasution, Kartika Zsaskia Nasution, M. Amin Nasution, Shamila Sufi Aulia Nazirah, Nazirah Ningtias, Anggitha Nst, Haris Munandar Nuraida Nuraida Nurhayati Nurhayati Nurliansyah, Nurliansyah Parapat , Anjarlit Romiyuliana Br Pitaloka, Syifa Amilia Puteri, Cut Intan Annisa Putri Khairani Putri Theresia Harianja Putriyana Putriyana Qisti Pristiwani Rafita Yuniarti Rahayu , Yayuk Putri Rahayu Rahayu Rahma Dona Rahmadani , Adevika Rahmadani Rahmadani Rahmah, Ruhiya Rahmasari, Siti Rahmayanti, Putri Reza Irma Ricky Andi Syahputra Ricky Andi Syahputra Rika Yuliana, Rika Riski Mulyani, Riski Risma Fauziah Pasaribu Rizki, Dewi Rizki, Rahmad Robiatun Rambe Rosaldi, Hikmah Rossa, Aliffa S Sumarlin Sagala , Vipi Zetiara Salwa, Shakhila Saragih, Alkausar Sari, Farida Sartika Daulay, Anny Sasnita, Merida Selfia Lestary Shilvia, Fatin Sinaga, Novita Yulianti Siradjuddin, Siradjuddin Siregar , Anisah Sitompul, M. Saleh Sitorus, Dara Indah Sri Wahyuni Syahfitri , Adelya Syahputra, Ricky Andi Syalsabila Putri Syarifah Aulia Tamara Army Tambupolon, Sanya Tanika Utama, Fazrul Amin Vera Estefania Kaban Virda Nurmazela Wijiyaningsih, Dinda Yanti, Rahma Yayuk Putri Rahayu Yulia Nanda Putri Yuniarti , Rafita Yuniarti, Rafita Ziza Putri Aisyia Fauzi Zulmai Rani