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Uji Aktivitas Antioksidan Teh Herbal Daun Melinjo (Gnetum Gnemon L.) Dengan Menggunakan Spektrofotometri Visibel Syarifah Aulia; Rafita Yuniarti; Gabena Indrayani Dalimunthe; Ridwanto Ridwanto
USADA NUSANTARA : Jurnal Kesehatan Tradisional Vol. 1 No. 2 (2023): Juli: USADA NUSANTARA
Publisher : Institut Nalanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47861/usd.v1i2.330

Abstract

Antioxidants are compounds that can counteract / reduce the negative effects of free radicals that cause damage to the body. Natural antioxidants are used as an alternative in an effort to minimize the use of synthetic antioxidants which can cause carcinogens. Utilization of melinjo leaves as herbal tea is one effort to use natural antioxidants to minimize the use of synthetic antioxidants which can cause carcinogens. The purpose of this study was to determine the content of secondary metabolites contained in melinjo leaf tea, the characteristics of melinjo leaf tea and the antioxidant activity of melinjo leaf tea. This research was conducted by making melinjo leaf herbal tea through a drying process in a drying cabinet at 40oC, phytochemical screening, examining the characteristics of the tea, and testing the antioxidant activity. The results of the Phytochemical screening examination showed that melinjo leaf herbal tea contains alkaloids, flavonoids, saponins, tannins, steroids/triterpenoids. As well as having the characteristics of water content 5.6866%, water extract 36.9740%, total ash content 5.7658%, water soluble ash content to total ash 7.7066, acid insoluble ash content 0.54%. While the antioxidant activity test of melinjo leaf herbal tea showed very weak antioxidant activity with an IC50 value of 501.7622 ppm
PERBANDINGAN KADAR PROTEIN PADA KUNING DAN PUTIH TELUR BEBEK REBUS MENGGUNAKAN METODE KJELDAHL DAN SPEKTROFOTOMETRI VISIBLE M. Naufal Rifqi; Daulay, Anny Sartika; Ridwanto; Rafita Yuniarti
FARMASAINKES: JURNAL FARMASI, SAINS, dan KESEHATAN Vol. 4 No. 1 (2024): FARMASAINKES: JURNAL FARMASI, SAINS dan KESEHATAN
Publisher : Fakultas Farmasi Universitas Muslim Nusantara Al Washliyah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32696/farmasainkes.v4i1.3350

Abstract

Pendahuluan: Salah satu telur unggas yang banyak dikonsumsi masyarakat adalah telur bebek Telur merupakan sumber protein hewani dan lauk pauk yang mudah didapat, murah, dan penuh nutrisi. Telur memberikan nutrisi lengkap yang diperlukan untuk pertumbuhan sel. Telur juga menyediakan semua asam amino penting bagi manusia, yang membuatnya menjadi sumber protein berkualitas tinggi. Tujuan: Tujuan Penelitian ini adalah untuk mengetahui dan membandingan kadar protein dari kuning dan putih telur bebek rebus menggunakan metode kjeldahl dan spektrofotometri visible. Metode: Metode penelitian yang digunakan untuk mengukur kadar protein kuning dan putih telur bebek rebus menggunakan metode Kjeldahl dan spektrofotometri visible. Metode Kjeldahl meliputi destruksi, destilasi, dan titrasi, sedangkan metode spektrofotometri tampak meliputi pembuatan larutan biuret, penentuan panjang gelombang maksimum, pembuatan kurva standar, dan penentuan kandungan protein sampel. Hasil: Hasil penelitian didapat kadar protein pada telur rebus dengan metode kjeldahl yaitu kuning telur bebek rebus sebesar 9,5363% dan putih telur bebek rebus sebesar 11,6497%. Dengan metode spektrofotometri visible yaitu kuning telur bebek rebus sebesar 8,4794% dan putih telur bebek rebus sebesar 8,64375%. Terdapat perbedaan nyata kadar protein antara metode kjeldahl dengan spektrofotometri visible dengan hasil SPSS nilai Sig. 0,000 < 0,05.
Uji Sitotoksisitas Ekstrak Etanol Daun Bayam Duri (Amaranthus spinosus L.) dengan Metode Brine Shrimp Lethality Test (BSLT) Fauzi, Ziza Putri Aisyia; Ridwanto, Ridwanto; Rani, Zulmai; Arifin, Khairil Fikri
Journal of Pharmaceutical and Health Research Vol 5 No 2 (2024): June 2024
Publisher : Forum Kerjasama Pendidikan Tinggi (FKPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47065/jharma.v5i2.5398

Abstract

Free radicals can cause chronic disease, it takes years for the disease to become real or accumulative. One possibility that can occur as a result of the work of free radicals such as cancer. Cancer is one of the non-communicable diseases that is a public health problem, both in the world and in Indonesia. One of the plants that has the potential as an anticancer is Amaranthus spinosus L. The results showed that thorn spinach leaves contain strong antioxidants. This study aims to determine the class of secondary metabolites contained in the ethanolic extract of thorn spinach leaves and the cytotoxicity of the ethanolic extract of A.spinosus by looking at the LC50 value which was tested using the Brine Shrimp Lethality Test (BSLT) method. This research includes phytochemical screening of A.spinosus extract and Brine Shrimp Lethality Test (BSLT) method by looking at the number of larvae mortality of Artemia salina leach (LC50). The results of the phytochemical screening test showed that A.spinosus were positive for flavonoids, alkaloids, saponins, tannins, steroids and glycosides. The results of the cytotoxicity test using probit analysis showed an LC50 value of 269.15 g/mL, so it can be concluded that the ethanolic extract of A.spinosus is toxic and has potential as an anticancer.
Uji Aktivitas Antioksidan Ekstrak Etanol Daun Gandarusa (Justicia gendarussa Burm. F.) di Daerah Sibolga, Sumatera Utara Dengan Metode DPPH (1,1-Diphenyl-2-Picrylhydrazil) Harahap, Ade Try Atwinda; Ridwanto, Ridwanto
Journal of Health and Medical Science Volume 3 Nomor 1 Januari 2024
Publisher : CV. Pusdikra Mitra Jaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51178/jhms.v3i1.1874

Abstract

Tumbuhan gandarusa yaitu untuk pengobatan. Bagian tanaman yang digunakan adalah daun dan akar baik yang segar ataupun yang telah dikerinbgkan. Daunnya berkhasiat untuk mengatasi batuk, asma, nyeri lambung, rematik sendi, nyeri pinggang (encok), obat pening dan obat untuk haid yang tidak teratur. Kegunaan yang lain untyuk obat luka trpukul (memar), patah tulang, reumatik pada persendian, bisul, borok dan korengan. Tujuan:Tujuan penelitian ini untuk mengetahui aktivitas antioksidan ekstrak etanol daun Gandarusa dan untuk mengetahui golongan senyawa dalam daun gandarusa dan juga nilai IC50. Ekstrak didapat dengan cara maserasi menggunakan pelarut etanol 96%. Metode: Ekstrak etanol daun Gandarusa diuji menggunakan metode DPPH (1,1-Diphenyl-2-Picrylhydrazil), dan menggunakan spektrofotometri UV - Visible untuk menentukan panjang gelombangnya. Hasil skrining fitokimia bahwa serbuk simplisia daun Gandarusa mengandung golongan senyawa alkaloid, flavonoid, saponin, triterpenoid, dan tannin. Hasil pengujian aktivas antioksidan dalam meredam radikal bebas DPPH menunjukkan bahwa ekstrak etanol daun Gandarusa memiliki aktivitas antioksidan kategori kuat dimana ekstrak etanol daun Gandarusa memiliki nilai IC50 sebesar 59,46 ppm. Kesimpulan:Hasil pengujian aktivasi antioksidan dalam meredam radikal bebas DPPH menunjukkan bahwa ekstrak etanol daun Gandarusa memiliki aktivitas antioksidan kategori kuat.
ANTIOXIDANT ACTIVITY TEST OF JENGKOL LEAF EXTRACT (Archidendron pauciflorum (Benth.) I.C. Nielsen) USING DPPH (1,1-diphenyl-2-picrylhydrazyl) METHOD Manurung, Aisyah Baddriah; Ridwanto
International Journal of Public Health Excellence (IJPHE) Vol. 2 No. 1 (2022): June-December
Publisher : PT Inovasi Pratama Internasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55299/ijphe.v2i1.293

Abstract

Jengkol leaf (Archidendron Pauciflorum (Benth.) I.C.Nielsen) contains alkaloids, tannins, saponins, flavonoids, steroids/triterpenoids. The objective of this research was to determine the class of chemical compounds contained in simplisia and jengkol leaf extract as well as the antioxidant activity of jengkol leaf extract. The research stages include the collection and processing of simplicia, the manufacture of extracts by maceration with 96% ethanol solvent. Phytochemical screening of jengkol leaf powders and extracts includes examination of alkaloid compounds, tannins, saponins, flavonoids, and steroids. Examination of the characteristics of simplicia powder. The antioxidant and extract activity test of jengkol leaf extract was carried out using the DPPH method (1,1-diphenyl-2-picrilhydrazyl), where DPPH absorption was measured using a Vis spectrophotometer at a wavelength of 516 nm. The results of phytochemical screening, powders and simplician extracts contain alkaloid compounds, tannins, saponins, flavonoids, steroids / triterpenoids. The results of the examination of the characteristics of simplicia powder obtained a moisture content of 6.66%, a water-soluble juice content of 16.66%, an ethanol soluble juice content of 26.66%, a total ash content of 1.3%, an acid insoluble ash content of 0.42%. The results of measuring the antioxidant activity of jengkol leaf extract showed the strength of the "very strong" category with an IC50 value of 2.4807 ppm.
PENETAPAN KADAR FENOLIK TOTAL EKSTRAK METANOL KAYU KUNING (Arcangelisia flava (L.) Merr.) DENGAN BERBAGAI KONSENTRASI METANOL DETERMINATION OF TOTAL PHENOLIC CONTENT OF YELLOW WOOD (Arcangelisia flava (L.) Merr.) Methanol EXTRACT WITH VARIOUS METHANOL Hasanah, Qori; Daulay, Anny Sartika; Ridwanto, Ridwanto; Nasution, Haris Munandar
Medic Nutricia : Jurnal Ilmu Kesehatan Vol. 8 No. 3 (2024): Medic Nutricia : Jurnal Ilmu Kesehatan
Publisher : Cahaya Ilmu Bangsa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5455/nutricia.v8i3.6085

Abstract

Salah satu tanaman yang mengandung senyawa metabolit sekunder yaitu kayu kuning (Arcangelisia flava (L.) Merr.) yang terkandung dalam batangnya adalah alkaloid, fenolik, flavonoid saponin, dan tanin. Senyawa fenolik mempunyai aktivitas sebagai antioksidan. Senyawa fenolik adalah senyawa kimia yang memiliki ikatan rangkap terkonjugasi dan gugus kromofor dapat ditentukan kadarnya menggunakan metode spektrofotometri. Perbedaan dari metode dan penggunaan pelarut untuk menyari ekstrak berbeda dapat mempengaruhi kadar dan jenis senyawa fenolik yang akan diperoleh. Tujuan dari penelitian ini adalah untuk mengetahui kandungan metabolit sekunder dan kandungan total kadar fenolik dari ekstrak metanol kayu kuning dengan berbagai konsentrasi dari pelarut methanol 99,8%, 70% dan 50% menggunakan Spektrofotometri UV–VIS. Hasil skrining fitokimia pada ekstrak metanol kayu kuning bahwa terdapat kandungan golongan senyawa kimia seperti alkaloid, flavonoid, fenolik, tanin, steroid/triterpenoid, saponi dan glikosida. Penentuan kadar fenolik total dilakukan dengan menentukan panjang gelombang maksimum asam galat, operating time, pengukuran kurva kalibrasi asam galat dan perhitungan kadar fenolik total dengan menggunakan metode spektrofotometri UV-Vis. Hasil penentuan kadar fenolik total pada ekstrak metanol kayu kuning 99,8% sebesar 81,61002 ± 0,248610176 mg GAE/g sampel, 70% sebesar 68,8756 ± 0,24837736 mg GAE/g sampel dan 50% sebesar 57,7894 ± 0,13110763709 mg GAE/g sampel. One of the plants that contains secondary metabolite compounds is yellow wood (Arcangelisia flava (L.) Merr.), which contains alkaloids, phenolics, flavonoids, saponins and tannins in its stems. Phenolic compounds have activity as antioxidants. Phenolic compounds are chemical compounds that have conjugated double bonds and chromophore groups that can be determined using spectrophotometric methods. Differences in methods and use of solvents to extract different extracts can influence the levels and types of phenolic compounds that will be obtained. The aim of this research was to determine the secondary metabolite content and total phenolic content of yellow wood methanol extract with various concentrations of 99.8%, 70% and 50% methanol solvent using UV-VIS Spectrophotometry. The research results Phytochemical screening of methanol extract of yellow wood showed that it contained chemical compounds such as alkaloids, flavonoids, phenolics, tannins, steroids/triterpenoids, saponi and glycosides. Determination of total phenolic content was carried out by determining the maximum wavelength of gallic acid, operating time, measuring the gallic acid calibration curve and calculating total phenolic content using the UV-Vis spectrophotometric method. The results of determining the total phenolic content in 99.8% methanol extract of yellow wood were 81.61002 ± 0.248610176 mg GAE/g, 70% were 68.8756 ± 0.24837736 mg GAE/g and 50% were 57.7894 ± 0 .13110763709 mg GAE/g sample).
PENETAPAN KADAR MINERAL KALSIUM DAN MAGNESIUM PADA LABU SIAM (Sechium edule (Jacq.) Swartz) MENGGUNAKAN METODE SPEKTROFOTOMETRI SERAPAN ATOM Daulay, Anny Sartika; Ridwanto, Ridwanto; Rizki, Rahmad
Jurnal Farmanesia Vol 7 No 1 (2020): Jurnal Farmanesia
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jf.v7i1.2762

Abstract

Siamese pumpkin (Sechium edule (Jacq.) Swartz) is a plant originating from the cucurbitaceae tribe. This plant is a vegetable that grows in the subtropics that is used as food and at the same time used in medicine. Chayote contains secondary metabolites, minerals, and vitamins. The minerals contained in chayote include potassium, calcium, magnesium, sodium, phosphorus, zinc, iron and manganese. The purpose of this study was to determine the mineral content of potassium, calcium, and magnesium from old and young chayote and to determine the difference in mineral content of young and old chayote. The sample was taken by purposive sampling from Jalan Garu 2, Harjosari II Village, Medan Amplas District, North Sumatra. The sample consisted of old chayote and young chayote. Samples were treated with a wet digestion process. The assay was carried out using atomic absorption spectrophotometry with an air-acetylene flame. Quantitative analysis of potassium, calcium and magnesium was carried out at wavelengths of 766.5 nm, 422.7 nm and 285.2 nm, respectively. The results showed that the mineral levels of potassium, calcium, and magnesium in old chayote were (11.5926 -+ 2.2124) mg/100g, (31.1145 -+ 1.8648) mg/100g, (4.0623 -+ 2.6156) mg/100g. The mineral levels of potassium, calcium and magnesium in young chayote were (16.2461 -+ 2.2547) mg/100g, (16.5038 -+ 1.9765) mg/100g, (5.7721 -+ 0.1935), respectively. mg. There is a difference between the mineral content found in old chayote and young chayote with potassium and magnesium levels in old chayote being lower than young chayote and calcium levels in old chayote being higher than young chayote.
PENETAPAN KADAR MINERAL BESI DAN KALIUM PADA DAUN SINGKONG (Manihot esculenta Crantz) MENGGUNAKAN METODE SPEKTROFOTOMETRI SERAPAN ATOM Ridwanto, Ridwanto; Dalimunthe, Gabena Indrayani; Isnaria, Vita
Jurnal Farmanesia Vol 7 No 2 (2020): Jurnal Farmanesia
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jf.v7i2.2773

Abstract

Fresh vegetables are foodstuffs that contain lots of vitamins and minerals that are important for the body (Ayu, 2002). There are two types of cassava leaves, namely, ordinary cassava leaves and curly cassava leaves. Cassava leaves contain minerals and vitamins, including calcium, iron, magnesium, protein, fat, carbohydrates, phosphorus, vitamin A, vitamin B and vitamin C. This study aims to determine the mineral levels of iron, calcium and magnesium in ordinary cassava leaves. This research includes a dry destruction process and then quantitative analysis of iron, calcium, and magnesium using the atomic absorption spectrophotometry (AAS) method, namely ferrous metal at a wavelength of 248.3 nm, calcium metal at a wavelength of 422.7 nm and magnesium metal at a wavelength of 248 nm. 285.2 nm wave. The advantages of this method are selective, sensitive and simple. The results showed that the mineral content in cassava leaves were iron (4.1384 -+ 0.2.607) mg/100g, calcium (8.058 -+ 9.0230) mg/100 and magnesium (4.096 -+ 0.0351) mg/100g.
PENENTUAN KADAR DAN VALIDASI METODE SPEKTROFOTOMETRI ULTRAVIOLET PADA SEDIAAN TABLET BROMHEKSIN DARI BEBERAPA INDUSTRI FARMASI Miswanda, Dikki; Ridwanto, Ridwanto; Mulyani, Riski
Jurnal Farmanesia Vol 8 No 1 (2021): Jurnal Farmanesia
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jf.v8i1.2781

Abstract

Bromhexine works by increasing the amount of mucus and reducing its viscosity. In Pharmacopoeia Edition V (2014) there is no monograph of bromhexine either in syrup form or in tablet preparation. Bromhexine has chromophore and auxochrome groups in its structure so that this compound can absorb radiation in the ultraviolet (UV) region. The purpose of this study is to find an alternative method that is simple and inexpensive but provides good accuracy and precision. This method was validated and then the validated method was applied to the assay of bromhexine tablets on the market. The method used was ultraviolet spectrophotometry with 0.1 N HCl as a solvent. The sample used was 5 bromhexine tablets with trade names. The validation test parameters carried out are accuracy, precision, detection limits and quantitation limits. The results of the specified validation test provide an accuracy test with a percent recovery of 101.14% and a Relative Standard Deviation (RSD) of 1.6964%. This shows that this method provides good accuracy and precision, with a limit of detection (LOD) of 0.6315 and a limit of quantitation (LOQ) of 1.1983. The assay results of the tablet sample of the trade name Bisolvon (PT. Boehringer Ingelheim) 100.70% -+ 0.56%; Bromifar (PT. Ifars) 101.09% -+ 0.44%; Bronchitis (PT. Graha Farma) 100.65% -+ 0.67%; Mucohexin (PT. Sanbe) 100.92% -+1.25% and Omesolvon (PT. Mutifa) 100.34% -+ 0.37%. All tablets determined to meet the requirements of the general content of a tablet that is not less than 90% and not more than 110% of the amount stated on the label.
UJI AKTIVITAS ANTIBAKTERI DAUN SENGGANI (Melastoma candidum D.Don) DENGAN VARIASI EKSTRAKSI MASERASI DAN MICROWAVE ASSISTED EXTRACTION TERHADAP S. aureus DAN E. coli Hasibuan, Supia Indah; Nasution, Muhammad Amin; Ridwanto; Nasution, Haris Munandar
Jurnal Kesehatan Tambusai Vol. 5 No. 3 (2024): SEPTEMBER 2024
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jkt.v5i3.33460

Abstract

Penyakit infeksi merupakan salah satu permasalahan kesehatan yang sering terjadi.  Jenis infeksi bisa dipicu oleh bakteri gram postif maupun gram negatif. Salah satu mikroorganisme yang dapat menyebabkan infeksi yaitu Staphylococcus aureus dan Escherichia coli. Penelitian ini bertujuan mengidentifikasi senyawa metabolit sekunder dalam ekstrak etanol daun Senggani (Melastoma candidum D.Don) dan mengevaluasi aktivitas antibakterinya terhadap Staphylococcus aureus dan Escherichia coli. Jenis penelitian yang dilakuakan adalah penelitian eksperimental. Sampel tumbuhan yang digunakan dalam penelitian ini adalah daun senggani (Melastoma candidum D.Don) yang diperoleh dari Kabupaten Tapanuli Tengah. Metode pengambilan sampel dengan metode purposive yaitu tanpa membandingkan tumbuhan serupa didaderah lain. Data yang diperoleh dengan pengukuran diameter zona hambat ekstrak etanol daun senggani (Melastoma candidum D.Don) dengan metode ekstraksi maserasi dan microwave assisted extraction dari masing-masing konsentrasi dengan 3 kali pengulangan. Data yang diperoleh dianalsis dengan menggunakan uji Oneway Annova. Parameter yang digunakan dalam penelitian ini adalah uji skrining fitokimia, metode ekstraksi maserasi dan microwave assisted extraction dan uji antibakteri. Hasil penelitian menunjukkan bahwa ekstrak daun Senggani yang diperoleh melalui metode maserasi mengandung alkaloid, flavonoid, saponin, tanin, dan steroid. Ekstrak dari metode MAE juga mengandung senyawa-senyawa tersebut, serta glikosida tambahan. Aktivitas antibakteri ekstrak daun Senggani terhadap Staphylococcus aureus dan Escherichia coli menunjukkan bahwa ekstrak dari metode MAE memberikan zona hambat yang lebih besar dibandingkan dengan ekstrak dari metode maserasi, menunjukkan efektivitas yang lebih tinggi dalam aktivitas antibakteri.
Co-Authors Abdullah, Husaini Adawiyah, Marissa Tasya Adyani Maulinda Afrizani, Afrizani Agus Priyadi Akbar, Windi Hari Al Hudawi, Muhammad Al Qodri, Muhammad Alviana, Liya Amin Nasution, Muhammad Amir, Annisa Fadilah Andi Syahputra, Ricky Ani Sutiani Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Aprilia, Hijjatun Aprilia, Medhy Ardhita Arifin, Khairil Fikri Arjuniadi, Arjuniadi Audry Pratiwi Audry Pratiwi Azhar, Gustika Azri, Atika Azzahra, Fadya Bangun Lubis Cici Andriani Claudy Friesta Melanie Dahlia Hasibuan Dalimunthe, Gabena Indriyani Damanik, Sumayyah Daniel Happy Putra Daulay , Anny Sartika Daulay, Ani Sartika Daulay, Anny S. Denni Bahari Dikki Miswanda Dina Suciati Saragih Dison Silalahi, Alistraja Elma Natasya Endang D. Hamid Fadhilah M, Ihsan Fadhlah Al-Uyun Nasution Fajri, Fajrilda Aqilah Fathur Rahman Fathur Rahman Fathur Rahman Harun Fatur Rahman Fithri Pulungan, Ainil FRANSISKA, EVA Gabena Indrayani Dalimunthe Hafizha, Putri Hakimi Hakimi Harahap, Ade Try Atwinda Harahap, Siti Salimah Harahap, Sumiyarni Haris Munandar Nasution Harun , Fatur Rahman Harun, Fathur Rahman Hasanah, Qori Hasibuan , Nabila Hasim Hasibuan, Sarinah Hasibuan, Supia Indah Hazizah, Mautia Hermawan, Fiqri Humairah, Dwina Angelina Imelda Natasia Br S Meliala Indra B. Hutagalung Irvan Andreansyah Irwan Misbach Isnaria, Vita Khairunnisa Khairunnisa Lastri Afni Lince Bulutoding Lubis, Minda Sari M. Naufal Rifqi Maghfirah , Dini Makhfirah Malahayati Malahayati Mambang, D Elysa Putri Manik, Umi Chairani Manurung, Aisyah Baddriah Maryanti Yuza Maya Syafira Melinda, Reni Meliyana Meliyana Merani Phaustina Lumban Gaol Mirna Mirna Muchlis, Syaiful Muhammad Alfariz Baihaqi Siregar Muhammad Amin Nasution Muhammad Wahyuddin Abdullah Muhammad Wahyudi Munthe, Ariska Munthe, Herlina Nada, Dina Qatrun Nadila Indra Sepvina Nasri Nasri, Nasri Nasution , Muhammad Amin Nasution, Kartika Zsaskia Nasution, M. Amin Nasution, Shamila Sufi Aulia Nazirah, Nazirah Ningtias, Anggitha Nst, Haris Munandar Nur Ainun Nuraida Nuraida Nurhayati Nurhayati Nurliansyah, Nurliansyah Nurmazela, Virda Parapat , Anjarlit Romiyuliana Br Pasaribu, Fatin Fairuz Pitaloka, Syifa Amilia Pulungan, Ainil Fitri Puteri, Cut Intan Annisa Putri Intan Sari Putri Khairani Putri Theresia Harianja Putri Yupa Hasibuan Putriyana Putriyana Qisti Pristiwani Rahayu , Yayuk Putri Rahayu Rahayu Rahma Dona, Rahma Rahmadani , Adevika Rahmadani Rahmadani Rahmah, Ruhiya Rahmasari, Siti Rahmayanti, Putri Rambe, Robiatun Reza Irma Ricky Andi Syahputra Rika Yuliana, Rika Riski Mulyani, Riski Risma Fauziah Pasaribu Rizki, Dewi Rizki, Rahmad Rosaldi, Hikmah Rossa, Aliffa S Sumarlin Sagala , Vipi Zetiara Salwa, Shakhila Saragih, Alkausar Sari, Farida Sartika Daulay, Anny Sasnita, Merida Selfia Lestary Shilvia, Fatin Sinaga, Novita Yulianti Siradjuddin Siregar , Anisah Sitompul, M. Saleh Sitorus, Dara Indah Sri Wahyuni Syahfitri , Adelya Syahputra, Ricky Andi Syalsabila Putri Syarifah, Aulia Tamara Army Tambupolon, Sanya Tanika Trizaldi, Asep Utama, Fazrul Amin Vera Estefania Kaban Wijiyaningsih, Dinda Yanti, Rahma Yayuk Putri Rahayu Yulia Nanda Putri Yuniarti , Rafita Yuniarti, Rafita Ziza Putri Aisyia Fauzi Zulmai Rani