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All Journal Jurnal Pendidikan dan Pembelajaran Khatulistiwa (JPPK) JBIO: jurnal biosains (the journal of biosciences) Jurnal Filsafat Analisis: Jurnal Studi Keislaman Sosial Budaya Indonesian Journal of Pharmaceutical Science and Technology Edu Elektrika Journal International Journal of Applied Biology Sinkron : Jurnal dan Penelitian Teknik Informatika Jurnal Kesehatan Masyarakat Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) SemanTIK : Teknik Informasi BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) SOSIOHUMANIORA: Jurnal Ilmiah Ilmu Sosial dan Humaniora Jurnal Filsafat Jurnal Penelitian Pendidikan IPA (JPPIPA) Majalah Farmasi dan Farmakologi (Trends in Pharmacy and Pharmaceutical Sciences) JMM (Jurnal Masyarakat Mandiri) Kiryoku: Jurnal Studi Kejepangan Warta Perpustakaan Pusat Undip Biota Journal of Education, Humaniora and Social Sciences (JEHSS) Patrawidya: Seri Penerbitan Penelitian Sejarah dan Budaya Indonesian Journal for Social Responsibility Community Development Journal: Jurnal Pengabdian Masyarakat Pelita Masyarakat Jurnal Ilmiah Biologi UMA (JIBIOMA) Jurnal Teknik Informatika (JUTIF) Jurnal Ilmiah Wahana Pendidikan Jurnal Pengabdian kepada Masyarakat Journal of Public Health Innovation (JPHI) TEACHING : Jurnal Inovasi Keguruan dan Ilmu Pendidikan Narra J PROFICIO: Jurnal Pengabdian Masyarakat INDRA: Jurnal Pengabdian kepada Masyarakat JIDAN (JURNAL ILMIAH KEBIDANAN) Journal of Education Research Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Batara Wisnu : Indonesian Journal of Community Services Kasta: Jurnal Ilmu Sosial, Agama, Budaya dan Terapan Jurnal Novem Medika Farmasi Jurnal Pembelajaran dan Riset Pendidikan Journal of Business Economics and Management Jurnal Analisis Bisnis Ekonomi Jurnal bahasa, sastra, seni, dan pengajarannya
Sartini Sartini
Universitas Medan Area
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Other

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Anti-aging and immunomodulatory role of caffeine in Drosophila larvae Nainu, Firzan; Sartini, Sartini; Bahar, Muhammad A.; Asbah, Asbah; Rosa, Reski A.; Mudjahid, Mukarram; As'ad, Muhammad F.; Latada, Nadila P.
Narra J Vol. 4 No. 2 (2024): August 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i2.818

Abstract

Drug repurposing is a promising approach to identify new pharmacological indications for drugs that have already been established. However, there is still a limitation in the availability of a high-throughput in vivo preclinical system that is suitable for screening and investigating new pharmacological indications. The aim of this study was to introduce the application of Drosophila larvae as an in vivo platform to screen drug candidates with anti-aging and immunomodulatory activities. To determine whether Drosophila larvae can be utilized for assessing anti-aging and immunomodulatory activities, phenotypical and molecular assays were conducted using wildtype and mutant lines of Drosophila. The utilization of mutant lines (PGRP-LBΔ and Psh[1];;ModSP[KO]) mimics the autoinflammatory and immunodeficient conditions in humans, thereby enabling a thorough investigation of the effects of various compounds. The phenotypical assay was carried out using survival and locomotor observation in Drosophila larvae and adult flies. Meanwhile, the molecular assay was conducted using the RT-qPCR method. In vivo survival analysis revealed that caffeine was relatively safe for Drosophila larvae and exhibited the ability to extend Drosophila lifespan compared to the untreated controls, suggesting its anti-aging properties. Further analysis using the RT-qPCR method demonstrated that caffeine treatment induced transcriptional changes in the Drosophila larvae, particularly in the downstream of NF-κB and JAK-STAT pathways, two distinct immune-related pathways homologue to humans. In addition, caffeine enhanced the survival of Drosophila autoinflammatory model, further implying its immunosuppressive activity. Nevertheless, this compound had minimal to no effect on the survival of Staphylococcus aureus-infected wildtype and immunodeficient Drosophila, refuting its antibacterial and immunostimulant activities. Overall, our results suggest that the anti-aging and immunosuppressive activities of caffeine observed in Drosophila larvae align with those reported in mammalian model systems, emphasizing the suitability of Drosophila larvae as a model organism in drug repurposing endeavors, particularly for the screening of newly discovered chemical entities to assess their immunomodulatory activities before proceedings to investigations in mammalian animal models.
Dual effects of Camellia sinensis and Andrographis paniculata on hyperglycemia and infection in Drosophila Nainu, Firzan; Sartini, Sartini; Subehan, Subehan; Sari, Dwi K.; Bahar, Muhammad A.; Mudjahid, Mukarram; Latada, Nadila P.; Asbah, Asbah; Hardiyanti, Widya; Pratama, Muhammad R.; Suhenro, Suhenro
Narra J Vol. 5 No. 1 (2025): April 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i1.1972

Abstract

The coexistence of hyperglycemia and infectious diseases represents a critical global health challenge, particularly in resource-limited settings where it amplifies disease severity and complicates treatment approaches. Medicinal plants such as Camellia sinensis and Andrographis paniculata have gained recognition for their antioxidant, anti-inflammatory, and antimicrobial properties, making them promising candidates for addressing this double health burden. The aim of this study was to establish a preclinical model of hyperglycemia and infection (HI model) using Drosophila melanogaster and to investigate the therapeutic potential of C. sinensis and A. paniculata extracts in alleviating the burden associated with the HI condition. In this study, the HI model was established by simultaneously exposing D. melanogaster larvae to a high-concentration sucrose solution and Staphylococcus aureus for 24 hours. The larvae were then transferred to a high-sucrose diet supplemented with C. sinensis or A. paniculata extracts. Survival assays and molecular analyses were subsequently performed to evaluate the outcomes. Our findings revealed that the combination of hyperglycemia and infection significantly reduced survival rates in the Drosophila model. However, treatment with 1.25% C. sinensis and A. paniculata extracts notably improved survival, attributed to their antibacterial activity and regulation of key molecular pathways involved in immune responses, metabolic balance, and endogenous antioxidant defenses. These findings validate the utility of D. melanogaster as a model organism for investigating the double burden of HI. Furthermore, the study offers compelling evidence of the dual therapeutic potential of C. sinensis and A. paniculata in mitigating the detrimental effects of this condition. Overall, this research underscores the significant promise of plant-derived compounds in managing HI and paves the way for future studies to explore their underlying mechanisms and potential clinical applications.
Co-Authors Abdul Karim Agus Supriyoko Aisyah, Nurma Al Makin Al Makin, Al Makin Alpina Bukhori Amir, Muhammad Nur Amirullah -, Amirullah Amriani Amriani Andi Indardaya, Andi Andi Nilawati Usman andi nur zam zam Andi Saryoko Andriani Chondro Retno Anisantaria Anisantaria Anisantaria, Anisantaria Aprillia, Gita Dewi Ardepy, Sri Ardianto, Tiyo Arifin, Arfiani Ariska, Fitri Arjuna, Andi Artika, Cut Mutia As'ad, Muhammad F. Asbah, Asbah Askarulloh, Muhammad Azam Bahar, Muhammad A. Budiman, Ade Surya Cerianing Putri Pratiwi Chairunissa, Indri CHONDRO, ANDRIANI Daraini Mahdhalita Desilva Sindra Dewi Lestari Dewi Purwaningsih Dicky Kurniawan, Muhammad Djide, Nana Juniarti Natsir Djuretna Adi Imam Muhni, Djuretna Adi Imam Dodi Febrian Dwi ASTUTI E. Harso Kardhinata E. Harso Kardhinata Ellen Lumisar Panggabean Emilia Utomo, Emilia Endang Fauziati Eriani Sahara Eriani Sahara, Eriani Fahrianti, Nurmala Fastabiqul Khairad Ferdinand Susilo Fhahri Mubarak Firdaus, Thoriq Baihaqi Firzan Nainu Firzan Nainu Fitri, Nur Amalia Fitriani, Rita Gemini Alam Gemini Alam Green Juninas Simbolon Green Juninas Simbolon, Green Juninas Guspi Wilda Sari Sianipar Haeriah Haeriah, Haeriah Hafis Nurdin Haki, Ubay Handayani, Andriani Condro Retno Hardiyanti, Widya Harsono Harsono Harun Joko Prayitno Hayyun Maghfiroh Heddy Shri Ahimsa Putra Heni Febriani, Heni Henny Yuslina Herlina Lumbantobing Herlina Rante Herliyani, Selvi Hirjan, Muhamad Hormarita Saragih Husin, Farid I Gede Arya Sucitra Ida Fauziah Ida Fauziah, Ida Ilham Ramadhan Nasution Indri Rizki Rahmasari Irawan, Maya Novita Sari Irfayanti, Nur Alfiah Islami, Moch Zihad Islamy, M. Ali Nurhasan Jasmiadi Jasmiadi Juliana Ruminta Sijabat Kardhinata, E. Harso Karim, Abdul Khairani khairani khairani, Khairani KIKI NURTJAHJA Kristina Pangaribuan, Ingka Latada, Nadila P. Limbong, Lasmega R Listrahot Tondang Lulu Fatma Dewi Luwiyanto, Luwiyanto M.Natsir Djide Ma'ruf, Dedy Maharani, Berliana Intan Mamlukah, Mamlukah Mangilep, Muhammad Agung Ady Manullang, Widya PS. Marliana marliana marliana, Marliana Marti Silfia Masiroh, Masiroh Maya Novita Sari Irawan Meida Nugrahalia Meida Nugrahalia Meida Nugrahalia, Meida Mudjahid, Mukarram Mufidah Mufidah Muhammad Rahmatullah, Muhammad Muhammad Sanusi Muhammad Usman Muhammad Usman, Muhammad Muhibbin, Muhibbin Muhtar, Mohamad Khusnial Munir, Adelin Gusman Munte, Nuriana Nabila Na'ma Aisa Natsir Dijde, Nana Juniarti Natsir Djide Nisa, Aza Khiatun Novianti Novianti Nugrahalia, Meida Nur, Fitri Amalia Nuraini Nuraini Nurdin, Fatmawati Nurhalimah Nurhalimah Nuriana Munte Nurwijayanti Ocsanda, Devina Oki Irawati Okianna Okianna One May Sunday Nababan Pahlawi, Ilham Pangestu, Dwi Agung Panggabean, Ellen L. Pratama, Muhammad R. Pratama, Winda Setya Purnama Dewi Rohana Purnamasari, Eka Purnawanti, Dia Purwanti, Anik Rahmiati Rahmiati Rahmiati Rahmiati Ratnawati ratnawati ratnawati, Ratnawati Retna Astuti Kuswardani Retno Sari Rita Fitriani Ritonga, Triana Permatasari Riyanto Riyanto Riyanto Riyanto Riyanto Rizal Fahmi Rosa, Reski A. Rosliana Lubis Royman S Panjaitan Rr Yudiswara, Permatasari Ayu Saipul Sihotang Saputra, Dimas Aji Saputra Sari, Dwi K. Saripudin Saripudin Selamat, Iffah Liyana Binti Shodiq Purnomo Sigit Haryanto Sinaga, Martaulina Siregar, Rahma Sari Siregar, Siti Aisah Siti Nurhasanah Nugraha Siti Rubiatik Siti Rubiatik, Siti Situmorang, Kartika Soviyanti, Evi Subehan Subehan, Subehan Suhenro, Suhenro Sukoso Sukoso Sulastri Sulastri Sulastri Sulastri Sulfiana, Baiq Sumardi . Sumarna Sumarna Suryadithia, Rachmat Suwitno, Nurmadani Tarigan, Emilia Surabina Br Veni Hadju Vera Ananda Harahap Verry Riyanto Viranthy Surbakti, Diana Wajdi, Muhammad Farid WITRIANA ENDAH PANGESTI Yana Iqbal Maulana Yeni Artika Yuslina, Henny Zebua, Oktaviani Zebua, Winda Irawati