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Journal : IJOBI

 1 
MOLECULAR IDENTIFICATION OF A LOCAL LACTIC ACID BACTERIA (LAB) ISOLATE (B21) AND PRIMER CONFIRMATION FOR D-Lactate Dehydrogenase (D-LDH) GENE ISOLATION Madhani, Anisa Tiara; Nurcahyo, Heru; Octavia, Bernadetta; Astuti; Mercuriani, Ixora Sartika
Indonesian Journal of Bioscience (IJOBI) Vol. 1 No. 1 (2023): Indonesian Journal of Bioscience (IJOBI)
Publisher : Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v1i1.104

Abstract

This study aims to identify a lactic acid bacteria (BAL) isolated from native chicken (Gallus gallus domesticus) intestinum (B21) based on 16S rRNA gene sequence and also to confirm primers for PCR-based D-Lactate dehydrogenase (D-LDH) gene isolation. The genomic DNA of B21 was isolated then amplified using 16S rRNA. The PCR product then sequenced and aligned. The sequencing was done in 1st Base Pte. Malaysia. The sequence then aligned using the Basic Local Alignment Search Tool (BLAST) in NCBI to know the similar genome species. Isolation D-LDH gene was done through PCR using 5 primers (idb 0101, idb 1147, idb 0813, idb 1010, and idb 2021) of B21 and also J15 isolate genome. The PCR product were then sequenced and aligned with the D-LDH gene sequences in NCBI. The results of molecular identification based on 16S rRNA markers showed that B21 has 99.45% similarity to Bacillus proteolyticus. Amplification of the LDH gene with idb 1010 primer on J15 isolate yielded 4 fragments (i.e. 650, 1400, 1500, and 1700bp). The results of phylogenetic tests showed that the 1700bp fragment has a high similarity to the D-LDH gene in Myobacterium tuberculosis species with a bootstrap value of 84%. While other size fragments cannot be trusted to have similarity to LDH sequence because they have not reached the minimum bootstrap value that meets the qualifications.
Co-Authors A. Fadhil Desafa A. Hidayat Abdul Rahman Manga Adityawarman Hidayat Adrial, Rico Afdhal Muttaqin Afrinaldy Rustam, Afrinaldy Afrizal Ahmadi, Devila Laura Alviona Alimin Mahyudin Amelia Nur Vidyanti Amin Ananda Galang Wangsa Ananda Kurnia Putri Ariesta, Ria Arif Budiman Astri Gianatun Naziah Astuti Rahmayani Ayatullah B Erdiansyah Putra Bagas Taqwa Bahasa Indonesia Bahasa Indonesia, Bahasa Indonesia Bahfie Bernadetha Rizki Kaize Bernadetta Octavia Bhujangga Ayu Putu Priyudhahari Birawidha Bunga Ervinasari Bustomi Christine D. Nainggolan Dahyunir Dahlan Dedi Mardiansyah Desy Kurniawati Dewi Puji Rahayu Dian Fitriyani Dwi Amanda Dwi Pujiastuti Dwi Puryanti Dwi Yanti Elly Susanti Elvaswer Ernest Grace Evy Oktaviani Fauzi Fadlun Fahrurozi Fatma Afrianti Gobel Febriani, Suci Feriska Handayani Fiqi Diyona Fita Rahmawati Fitri Amelia Hermansyah Hafizah Risali Haldis Alvaro Halim Simatupang Hanipah, Sri Harti, Annisa Henni Indrayani Herlina Heru Nurcahyo Ika Juliana, Ika Ike Kurniawati Ilahi , Rizky Imam Taufiq Irfan Firdaus IRWANTO Ixora Sartika Mercuriani Izzah Tujannah Jaenab Julianis, Juli Julianto Hutasuhut JUNAID GAZALIN Karo Karo Karo, Alyssa Zahra Br Kasman Ediputra, Kasman Khairul Azwar Kusmanto, Allifia Maharani Putri Lamhot Parulian Manalu, Lamhot Parulian Lieung, Karlina Wong Lina Suryani Lussy Midani Rizki M. Ali Shafii Madhani, Anisa Tiara Madita, Parina Mahmuzar Mahuze, Paustina Ngali Mahyarni - Marpaung, Putri Lasmida Maulidina, Allisa Meflinda Mega Suteki Meqorry Yusfi Meri Sandora Miftahur Rahman Mimis Saputra Mochamad Guntur Molli Wahyuni Molly Wahyuni, Molly Muhaimin Muhammad Ihsan Narayani, Daliyah Nini Firmawati Noermanzah, Noermanzah Nofianti Novita Sari Nur Syafitri, Citra Nurhafid, Mohammad Nurhaliza Nurhidayah Sari, Nurhidayah Nursyahfira Nurul Jannah Nurwijayanti Olly Norita Tetra Pardiyanto, Eko Paskha Marini Thana Pinasti Utami, Pinasti Priyudahari, Bhujangga Putra, Ilham Chnadra Rahma Yenni Rahman, Rifki Habibi Ramacos Fardela Ramdhan Ratna Retno Wuri Sulistyowati Riady, Reza Muhammad Rimet, Rimet Rival Hanip Ruanda, Faldo Ody Rusmayadi Rusmi S. Yulianti Safina, Wan Dian Samsudin Duha Savitri Sekedar Halawa Sholeha, Herisa Hardiyanti singgih Prabowo Sita Agustina Siti Rohani Sity Rahmy Maulidya Sovia Lenny Sri Handani Syafitri, Marliza Syaira Amelya Syairah, Anisa Tina Lestari Trengginas Eka Putra Ufianah Wahidah Yeti, Febru Dekmi Zozy Aneloi Noli ZULFAH Zulfi Zulhaida Zulhendri