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All Journal Pendas : Jurnah Ilmiah Pendidikan Dasar Diksa : Jurnal Pendidikan Bahasa dan Sastra Indonesia Owner : Riset dan Jurnal Akuntansi Journal of Educational Research and Evaluation Jurnal Seni Nasional Cikini Jurnal Ilmiah Wijaya BERNAS: Jurnal Pengabdian Kepada Masyarakat International Journal Of Science, Technology & Management (IJSTM) Jurnal PEKA (Pendidikan Matematika) Teaching and Learning Journal of Mandalika (Teacher) Griya Journal of Mathematics Education and Application International Journal of Community Service Jurnal Bisnis Mahasiswa Surplus: Jurnal Riset Mahasiswa Ekonomi, Manajemen, dan Akuntansi JOURNAL OF CHEMICAL NATURAL RESOURCES (JCNaR) Jurnal Pendidikan dan Pembelajaran Matematika Indonesia Jurnal Pengabdian Masyarakat dan Riset Pendidikan Asthadarma: Jurnal Pengabdian Kepada Masyarakat Journal of International Multidisciplinary Research Neurona Indonesian Scholar Journal of Nursing and Midwifery Science IJOBI Jurnal Sains Materi Indonesia Jurnal Pustaka Cendekia Pendidikan Indonesian Journal of Pharmacology and Therapy Mathematics Education Journal World Health Digital Journal (wolgitj) Jurnal Teras Pengabdian Masyarakat Journal of Artha Biological Engineering Jurnal Ilmiah Pendidikan Citra Bakti Jurnal Ekonomi, Manajemen, Akuntansi dan Keuangan
Astuti
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Journal : IJOBI

 1 
MOLECULAR IDENTIFICATION OF A LOCAL LACTIC ACID BACTERIA (LAB) ISOLATE (B21) AND PRIMER CONFIRMATION FOR D-Lactate Dehydrogenase (D-LDH) GENE ISOLATION Madhani, Anisa Tiara; Nurcahyo, Heru; Octavia, Bernadetta; Astuti; Mercuriani, Ixora Sartika
Indonesian Journal of Bioscience (IJOBI) Vol. 1 No. 1 (2023): Indonesian Journal of Bioscience (IJOBI)
Publisher : Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v1i1.104

Abstract

This study aims to identify a lactic acid bacteria (BAL) isolated from native chicken (Gallus gallus domesticus) intestinum (B21) based on 16S rRNA gene sequence and also to confirm primers for PCR-based D-Lactate dehydrogenase (D-LDH) gene isolation. The genomic DNA of B21 was isolated then amplified using 16S rRNA. The PCR product then sequenced and aligned. The sequencing was done in 1st Base Pte. Malaysia. The sequence then aligned using the Basic Local Alignment Search Tool (BLAST) in NCBI to know the similar genome species. Isolation D-LDH gene was done through PCR using 5 primers (idb 0101, idb 1147, idb 0813, idb 1010, and idb 2021) of B21 and also J15 isolate genome. The PCR product were then sequenced and aligned with the D-LDH gene sequences in NCBI. The results of molecular identification based on 16S rRNA markers showed that B21 has 99.45% similarity to Bacillus proteolyticus. Amplification of the LDH gene with idb 1010 primer on J15 isolate yielded 4 fragments (i.e. 650, 1400, 1500, and 1700bp). The results of phylogenetic tests showed that the 1700bp fragment has a high similarity to the D-LDH gene in Myobacterium tuberculosis species with a bootstrap value of 84%. While other size fragments cannot be trusted to have similarity to LDH sequence because they have not reached the minimum bootstrap value that meets the qualifications.
Co-Authors A. Fadhil Desafa A. Hidayat Abdul Rahman Manga Adhitya, Tiara Adityawarman Hidayat Adrial, Rico Afdhal Muttaqin Ahmadi, Devila Laura Alviona Alimin Mahyudin Amelia Nur Vidyanti Amin Ananda Galang Wangsa Ananda Kurnia Putri Ariesta, Ria Arif Budiman Aulya Fitri Ayatullah B Erdiansyah Putra Bagas Taqwa Bahasa Indonesia Bahasa Indonesia, Bahasa Indonesia Bahfie Bernadetta Octavia Birawidha Bunga Ervinasari Bustomi Christine D. Nainggolan Dahyunir Dahlan Dedi Mardiansyah Dian Fitriyani Dwi Amanda Dwi Pujiastuti Dwi Puryanti Dwi Yanti Elly Susanti Elvaswer Ernest Grace Evy Oktaviani Fauzi Fadhilaturrahmi Fadhilaturrahmi Fadlun Fatma Afrianti Gobel Febriani, Suci Feriska Handayani Fiqi Diyona Fita Rahmawati Fitri Amelia Hermansyah Fitri Sari, Fitri Gianatun Naziah, Astri Hafizah Risali Haldis Alvaro Halim Simatupang Harti, Annisa Hasibuan, Mahdanisa Herlina Heru Nurcahyo Ika Juliana, Ika Ilahi , Rizky Imam Taufiq Insani, Sri Ulfa Irfan Firdaus IRWANTO Ixora Sartika Mercuriani Izzah Tujannah Jaenab Julianis, Juli Julianto Hutasuhut JUNAID GAZALIN Karo Karo Karo, Alyssa Zahra Br Kasman Ediputra Kasman Ediputra, Kasman Khairul Azwar Kusmanto, Allifia Maharani Putri Lamhot Parulian Manalu, Lamhot Parulian Lina Suryani Lussy Midani Rizki M. Ali Shafii Madhani, Anisa Tiara Madita, Parina Marpaung, Putri Lasmida Maulidina, Allisa Meqorry Yusfi Miftahur Rahman Mimis Saputra Molli Wahyuni Molly Wahyuni, Molly Mufarizuddin Muhaimin Muhammad Ihsan Narayani, Daliyah Nasrul Nini Firmawati Noermanzah, Noermanzah Nofianti Novita Sari Nur Syafitri, Citra Nurhafid, Mohammad Nurhaliza Nurhaswinda Nurhidayah Sari, Nurhidayah Nursyahfira Nurul Jannah Nurwijayanti Olly Norita Tetra Pardiyanto, Eko Pinasti Utami, Pinasti Qadri, Uchi Rahma Yenni Rahman, Rifki Habibi Rahmayani, Astuti Ramacos Fardela Ramdhan Ratna Riady, Reza Muhammad Ruanda, Faldo Ody Rusmayadi S. Yulianti Safina, Wan Dian Samsudin Duha Savitri Sekedar Halawa singgih Prabowo Sita Agustina SITI HANIFAH Siti Rohani Sity Rahmy Maulidya Sovia Lenny Sri Handani Syafitri, Marliza Syaira Amelya Syairah, Anisa Tina Lestari Trengginas Eka Putra Ufianah Wahidah Winda Apriani Yostra Reza Zozy Aneloi Noli ZULFAH Zulfi Zulhendri