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All Journal Microbiology Indonesia Indonesian Journal of Human Nutrition Jurnal Biosains Pascasarjana Indonesian Journal of Tropical and Infectious Disease Majalah Kulit, Karet, dan Plastik BERITA BIOLOGI Dental Journal (Majalah Kedokteran Gigi) The Indonesian Biomedical Journal Health Notions Journal of Applied Food Technology Indonesian Journal of Medicine Jurnal Respirologi Indonesia Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia JPPS (Jurnal Penelitian Pendidikan Sains) JPPIPA (Jurnal Penelitian Pendidikan IPA) Majalah Biomorfologi (Biomorphology Journal) Jurnal Psikiatri Surabaya (Surabaya Psychiatry Journal) MAHESA : Malahayati Health Student Journal Berkala Penelitian Hayati Jurnal Pendidikan Ilmu Pengetahuan Alam (JP-IPA) Jurnal Natural Jurnal Keperawatan Soedirman (The Soedirman Journal of Nursing) Folia Medica Indonesiana

SOETJIPTO .

Departemen Biokimia Kedokteran, Fakultas Kedokteran, Universitas Airlangga, Surabaya

Author-ID : 215442

Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Education Energy Engineering Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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CHARACTERIZATION OF ENVELOPE E PROTEIN OF DENGUE VIRUS INDNESIA ISOLATE Rantam F A; Soetjipto Soetjipto; Dachlan Y P
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 5 No 2 (2000): June 2000
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/472

Dengue virus is a human pathogen that has as an increasingly important public health threat. These virus have four serotypes (DEN-1, DEN-2, DEN-3, DEN-4). There is no specific treatment for any pathogenic dengue virus and there are few vaccines available. This study was carry out to E protein characterization from dengue virus infected C5/36 cells for vaccine candidate. We found that E protein induced antibody response. The data of the reactivity on the nitrocellulose membrane between E protein against polyclonal and monoclonal antibodies on western blot analysis, shown that the E protein whisc was once of dengue virus protein has immunogenic. The Indonesia isolate had no difference with the Phillipine isolate. The result suggested that all of the E protein dengue virus strain have peptides overlapping. Also promising for he production of immunogenic particulate proteins, especially DEN protein for the four strains.

Sleep Disorders in Late-Life Depression Katharina Merry Apriliani; dr. Soetjipto
Jurnal Psikiatri Surabaya Vol. 9 No. 1 (2020): May
Publisher : Faculty of Medicine, Universitas Airlangga

Depression is an important mental health problem in developed societies, and as many as 50%-90% of patients with depression complain of sleep disorders. In the elderly, sleep disorders comorbid depressive disorders are getting worse. Late-life Depression (LLD) is often not detected because the symptoms are less specific than younger age. It even provides an independent risk factor impact on suicide ideas and behaviour. Causative risk factors of sleep disorders in Late-life Depression are biological and non-biological factors that form the basic of pharmacological and non- pharmacological management.

Clinical and Demographic Characteristics Differences between M. tuberculosis and M. bovis Infection in Bronchoalveolar Lavage Pulmonary TB Patients, Indonesia Budi Yanti; Soetjipto Soetjipto; Ni Made Mertaniasih; Muhammad Amin
Jurnal Respirologi Indonesia Vol 39, No 4 (2019)
Publisher : Perhimpunan Dokter Paru Indonesia (PDPI)/The Indonesian Society of Respirology (ISR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36497/jri.v39i4.76

Background: Some species of the Mycobacterium tuberculosis complex (MTBC) which can cause tuberculosis, particularly M. tuberculosis and M. bovis, may have different virulence property and therefore associated with various clinical severity in tuberculosis (TB) patients. The aim of this study was to determine the differences in clinical and demographic characteristics between M. tuberculosis and M. bovis infection among Indonesian TB patients. Methods: Thirty-one new and active TB patients were confirmed to have acid fast bacilli (AFB) sputum positive and/or Xpert MTB/RIF positive for M. tuberculosis from Dr. Soewandhie Hospital, Surabaya, Indonesia. Interviews were conducted to record the clinical and demographics required. The MTBC were isolated from bronchoalveolar lavage fluid (BALF) and determined by primer-specific PCR targeting TbD1 and RD9 region gene. The degree of lung tissue damage was classified using NICE Scoring System. Results: The MTBC were detected in all patients on whom 19 and 12 isolates were classified as M. tuberculosis and M. bovis respectively. There was a different on 74.2% of productive age subjects (21-50 years) with M. tuberculosis infection (P

HIV Genotype Analysis from HIV Infected Patients in East Java Area Yulia Sari Ismail; Soetjipto Soetjipto; Eddy Bagus Wasito; Nasronudin Nasronudin
Jurnal Natural Volume 12, Number 2, September 2012
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.358 KB)

Human Immunodeficiency Virus type 1 (HIV-1) has been known to cause Acquired Immune Deficiency Syndrome (AIDS) disease and has been alaso divided into several subtypes (A, B, C, D, F, G, H, J, K) and Circulating Recombinant Form (CRF). Different characteristics of subtype of the virus and its interaction with host can affect the severity of the disease. This study was aimed to analyze HIV-1 genotypes circulating in HIV/AIDS patients from the East Java region descriptively. Information from this research was expected to complement the data of mocular epidemiology of HIV in Indonesia. This study used blood plasma from patients who had been tested to be HIV positive who were seeking treatment or are reffered to the Intermediate Care Unit of Infectious Disease (UPIPI) Dr. Soetomo Hospital Surabaya from various area representing the East Java regions. Plasma was separated from blood samples by centrifugation for use in the the molecular biology examination including RNA extraction, nested PCR using specific primer for HIV gp120 env gene region, DNA purifying, DNA sequencing, and homology and phylogenetic analysis. Based on the nucleotide sequence of the HIV gp120 env gene, it was found that the most dominant genotypes in East Java belonged to one group of Circulating Recombinant Form (CRF), namely CRF01_AE and CRF3x_01B, which has been also found in Southeast Asia. In the phylogenetic tree, most of HIV samples (30 samples) were in the same branch with CRF01_AE and CRF3x_01B, except one sample (HIV40) was in the same branch with subtype B.

ANALYSIS OF HIV SUBTYPES AND CLINICAL STAGING OF HIV DISEASE/AIDS IN EAST JAVA Ismail, Yulia; Soetjipto, Soetjipto; Wasito, Eddy; Nasronudin, Nasronudin
Indonesian Journal of Tropical and Infectious Disease Vol. 3 No. 2 (2012)
Publisher : Institute of Topical Disease Universitas Airlangga

Human Immunodeficiency Virus type 1 (HIV-1) known to cause Acquired Immune Deficiency Syndrome (AIDS) disease are divided into several subtypes (A, B, C, D, F, G, H, J, K) and Circulating Recombinant Form (CRF). Different characteristics of subtype of the virus and its interaction with the host can affect the severity of the disease. This study was to analyze HIV-1 subtypes circulatingin HIV/AIDS patients from the East Java region descriptively and to analyze its relationship with clinical stadiums of HIV/AIDS. Information from this research was expected to complement the data of mocular epidemiology of HIV in Indonesia. This study utilited blood plasma from patients who had been tested to be HIV positive who sected treatment to or were reffered to the Intermediate Care Unit of Infectious Disease (UPIPI) Dr. Soetomo Hospital Surabaya from various area representing the East Java regions. Plasma was separated from blood samples by centrifugation for use in the the molecular biology examination including RNA extraction, nested PCR using specific primer for HIV gp120 env gene region, DNA purifying, DNA sequencing, and homology and phylogenetic analysis. Based on the nucleotide sequence of the HIV gp120 env gene, it was found that the most dominant subtypes in East Java were in one group of Circulating Recombinant Form (CRF) that is CRF01_AE, CRF33_01B and CRF34_01B which was also found in Southeast Asia. In the phylogenetic tree, most of HIV samples (30 samples) are in the same branch with CRF01_AE, CRF33_01B and CRF34_01B, except for one sample (HIV40) which is in the same branch with subtype B. HIV subtypes are associated with clinical stadiums (disease severity) since samples from different stages of HIV disease have the same subtype.

DETECTION OF TUMOR NECROSIS FACTOR- (TNF- ) GENE PROMOTERS POLYMORPHISM AMONG LIVER CIRRHOSIS PATIENTS WITH CHRONIC HEPATITIS B VIRUS (HBV) INFECTION IN SURABAYA, INDONESIA Wungu, Citrawati Dyah Kencono; Amin, Mochamad; Ruslan, S. Eriaty N.; Purwono, Priyo Budi; Kholili, Ulfa; Maimunah, Ummi; Setiawan, Poernomo Boedi; Lusida, Maria Inge; Soetjipto, Soetjipto; Handajani, Retno
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 5 (2019)
Publisher : Institute of Topical Disease Universitas Airlangga

Polymorphisms in TNF-α gene promoter region are known of its role in the production of TNF-α which may influences the pathogenesis of liver disease. SNPs in positions 238 and 308 of TNF-α gene promoters may affect the production of these cytokines. This study was aimed to detect Single Nucleotide Polymorphism (SNP) on -238 and -308 positions in the TNF-α gene promoter among liver cirrhosis patients with HBV infection in Surabaya, Indonesia. This was descriptive exploratory research with cross sectional study design using serum liver cirrhosis patients with HBV infection in Endoscopy Outpatient Clinic Dr. Soetomo General Hospital, Surabaya from April-May 2017. SNPs at -238 and -308 on TNF-α gene promoter (rs361525 and rs1800629 respectively) were detected using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) with primers specific for the TNF-α promoter region and restriction enzymes NcoI and MspI. The genotypes of TNF-α gene promoter were assessed according to the length of the fragments produced in RFLP. Serum TNF-α levels was measured by commercial ELISA. In this study, as much as 149 positive HBsAg patients was found in Endoscopy Outpatient Clinic, Dr. Soetomo General Hospital, Surabaya. From those amount, as much as 30 liver cirrhosis patients with positive HBsAg were obtained. From 2/30 (6.7%) patients showed the GA heterozygote SNP either position -238 or -308. No patient had the AA genotype. Median blood TNF-α level in women (38 ng / L) was higher than in men (33 ng / L). TNF-α levels in patients with GA heterozygote genotype at -238 and -308 in this research was not different than wild-type (GG genotype). Among patients with liver cirrhosis due to chronic HBV infection in Surabaya, Indonesia, Surabaya, we found GA polymorphisms the TNF-α promoter gene at positions -238 and -308 in 6.7% patients, and did not find homozygous AA polymorphisms. Further studies including larger numbers of patients from various ethnic backgrounds in Indonesia are needed to provide robust data on TNF-α gene promoter polymorphisms and their role in the pathogenesis of liver cirrhosis with HBV infection in this country.

Case of Recurrent Episode of Schizoprenia Paranoid in Pregnant Woman Setiobudi, Karen Delicia; Soetjipto, Soetjipto; Damba Bestari; Era Catur Prasetya
Jurnal Psikiatri Surabaya Vol. 12 No. 1 (2023): May
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jps.v12i1.34707

AbstractIntroductions: Pregnancy is a stressor to women, in terms of biological as well as psychological. For individuals who previously received medication for schizophrenia in the past, it can be a triggering factor for the relapse of the illness. Here we describe a case of a pregnant woman who experiences a relapse in pregnancy. Case: The patient was a 40-year-old pregnant woman, brought to the emergency room after attacking her father by stabbing her father's head with a knife. She was suspicious of her dad's gaze at her. She was hostile towards healthcare workers when being examined. For her pregnancy, consultation with the Obstetric and Gynaecology department had been done to ensure that she and her baby were taken care of while being treated in the psychiatry ward. Discussions: The patient was diagnosed as having paranoid schizophrenia recurrent episodes. There was no complication found in her pregnancy, and she was scheduled for Sectio Caesarean as she was not mentally fit to do vaginal delivery. The psychiatric approach to the patient was atypical antipsychotic along with supportive psychotherapy. Psychoeducation and family therapy were also done to ensure that the patient's family understand the illness and the importance of compliance with the medication. Conclusions: Pregnancy is one of the factors that lead to the relapse of schizophrenia in patients with a history of mental illness. Holistic treatment is needed to treat the patient to prevent the relapse of the illness.

Risk Factor Profile and Role of Cardiovascular Disease Outreach Program by Experts in Rural Communities: A Pilot Study in Magetan Regency, Indonesia Wungu, Citrawati Dyah Kencono; Elvaretta, Clarissa; Palupi, Dian Eka Roro; Qurnianingsih, Ema; Susilo, Hendri; Lukitasari, Lina; Humairah, Ira; Khaerunnisa, Siti; Soetjipto, Soetjipto; Handajani, Retno; Safitri, Indri; Ummah, Fithriyah Cholifatul; Waskito, Langgeng Agung; Prabowo, Gwenny Ichsan; Wiratama, Bayu Satria; Amin, Indah Binti Mohd
Jurnal Keperawatan Soedirman Vol 19 No 2 (2024): Jurnal Keperawatan Soedirman (JKS)
Publisher : Fakultas Ilmu-ilmu Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jks.2024.19.2.10947

Cardiovascular outreach programs and risk factors profiling have been regarded as key strategy in preventing and controlling cardiovascular diseases as the leading global causes of death worldwide, especially when being conducted by a professional. This pilot study aims to evaluate the effectiveness of a cardiovascular outreach program in Plaosan District, Magetan Regency, to improve knowledge and influence changes in the community’s behavior related to cardiovascular risk factors. This study was conducted by providing educational interventions and profiling cardiovascular risk factors on 90 adults using consecutive sampling method. Health education was performed by experts and evaluated by pre-post tests before and after the material delivery. Statistical analysis was used to assess the relationship of risk factors that can contribute to cardiovascular disease. This study showed a significant increase in the participants' knowledge score after the intervention (p = 0.007). There were several risk factors for cardiovascular disease in the participants, with the highest risk prevalence being diabetes mellitus (33.33%). Six participants (6.67%) had abnormal electrocardiography results. In conclusion, our study showed a high prevalence of cardiovascular risk factors in rural communities and the importance of cardiovascular outreach programs by experts to improve the understanding of cardiovascular disease in a specific community.

Pengembangan Perangkat Pembelajaran Fisika Berbasis Inkuiri Terbimbing untuk Melatihkan Keterampilan Berpikir Kreatif Siswa Widia, Widia; Soetjipto, Soetjipto; Ibrahim, Muslimin; Sarnita, Fitria; Saifullah, Saifullah
Jurnal Pendidikan Ilmu Pengetahuan Alam (JP-IPA) Vol 5, No 1 (2024): Mei 2024
Publisher : STKIP HARAPAN BIMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56842/jp-ipa.v5i1.284

Creative thinking skills are one of the skills that are very necessary in the 21st century, so it is important to train students. This research aims to develop a guided inquiry-based physics learning tool that is suitable for practicing creative thinking skills on static fluid material in SMA/MA. This research is development research using a 4-D model with an experimental type of research with a one-group pretest-posttest design. The learning tools developed include; (1) Learning Implementation Plan; (2) Student textbooks; and (3) Student Activity Sheet. Data collection techniques use tests, observation, and documentation using creative thinking skills test instruments, student activity observation sheets, and student response questionnaires. This research was conducted on 22 class XI students. Data analysis techniques use quantitative and qualitative descriptive to test the validity, practicality, and effectiveness of the data. The research findings are: 1) The learning tools developed are categorized as valid; 2) The practicality of learning tools can be seen from the implementation of the learning implementation plan and student activities carried out in accordance with the planned stages; 3) The effectiveness of learning tools is seen from (a) Students' creative thinking skills before being given treatment and after being given treatment, (b) Students' responses to the tools and implementation of learning. So it can be concluded that the guided inquiry-based physics learning tool developed is suitable for training students' creative thinking skills.

Reduction of Fructosamine Levels after Ramadan Fasting in Children with Type 1 Diabetes Mellitus Faizi, Muhammad; Rochmah, Nur; Marcos, Imella; Endaryanto, Anang; Soetjipto, Soetjipto
Indonesian Journal of Medicine Vol. 7 No. 2 (2022)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (31.885 KB)

Background: Ramadan fasting is one religious challenge for children with type 1 diabetes mellitus (T1DM) which required specific management and strict monitoring. Fructosamine, which reflects glycemic control within 2-3 weeks, is an ideal parameter of glycemic control in Ramadan fasting. This study aimed to analyzed the effect of Ramadan fasting on fructosamine level of children with T1DM.Subjects and Method: This prospective cohort study included children aged under 18 years old diagnosed wit T1DM for >6 months at Dr. Soetomo Hospital, Surabaya, Indonesia in Ramadan 1438 H (May-June 2017). The independent variable from this study was Ramadhan fasting and the dependent variables were fructosamine and HbA1c. The sampling technique was total sampling. Forty-two children were included and devided into 27 children in the fasting and 15 children in the non-fasting group. Fructosamine and HbA1c levels were measured before, mid- and after Ramadan and the comparison between groups were analyzed using the independent T-test.Results: The mean age of all the subjects was 8.95 (SD=3.2) years old, 47.6% were males. The mean (SD) HbA1c level of all the subjects was 10.42 (1.16) %. There was a mild reduction of mean fructosamine level after Ramadan in the fasting group. There was no significat differece I fructsamie level before (Mean= 556.8; SD= 180.39; p= 0.620), mid (Mean= 568.07; SD= 175.37; p= 0.520), and after Ramadhan between groups (Mean= 539.41; SD= 173.04; p= 0.410) respectively. The average fructosamine levels after Ramadan was positively and strongly related to HbA1c (r=0.69; p<0.001). There was no case of severe hypoglycemia and diabetic ketoacidosis in both groups.Conclusion: There is a reduction of mean fructosamine level after Ramadan fasting in children with T1DM although it isn’t statistically significant.Keywords: type 1 diabetes, children, fasting, Ramadan, fructosamineCorrespondence: Nur Rochmah. Department of Child Health, Dr. Soetomo General Hospital/ Faculty of Medicine, Universitas Airlangga. Mayjend Prof. Dr. Moestopo No. 6-8, Surabaya, East Java, Indonesia, 60286. Number: +6281703501118. Email: drnurrochmah@gmail.com.Indonesian Journal of Medicine (2022), 07(02): 131-137https://doi.org/10.26911/theijmed.2022.07.02.02

Co-Authors Abdurachman Abdurachman Adi, Ginanjar Sasmito Aksono HP., Eduardus Bimo Amin, Indah Binti Mohd Amin, Mochammad Aminuyati Anang Endaryanto Anggraeni, Zuhrotul Eka Yulis Annis Catur Adi Annisa Rizky Maulidiana Arumisore, Netty M Asnita, Asnita Aulanni'am, Aulanni'am Ayesie Natasha Zulka Bakhtiar, Arief Bakhtiar, Arief Bayu Satria Wiratama Budi Yanti Choirunisa, Latifatu Chorisma Permata Putri Nurazizah Citriana, Mirna Yunik Dachlan Y P Damba Bestari Dienanta, Savira Eddy Bagus Wasito Eddy Wasito, Eddy Elvaretta, Clarissa Ely Rahmatika Nugrahani Ely Rahmatika Nugrahani, Ely Rahmatika Era Catur Prasetya Fedik Abdul Rantam Fitria Sarnita G. Panggabean Ginanjar Sasmito Adi HAK HOTTA HANSON, J. Harianto Notopuro Hendri Susilo Hendy Hendarto Hermanto, Fery Herniyati HERNOMO ONTOSENO KUSUMOBROTO Huda, Khusnul Humairah, Ira Ida Bagus Narmada Indri Safitri Indri Safitri, Indri Juminten Saimin Juniastuti Juniastuti Katharina Merry Apriliani Khaerunnisa, Siti Kirana, Tjandra Lukitasari, Lina Lukitasari, Lina Luqman Hakim Andira M. THAMRIN HIDAYAT Machin, Abdulloh Maghfiroh, Faridatul Marcos, Imella MARIA INGE LUSIDA Maria Inge Lusida MOCHAMAD AMIN Mufiannoor, Eddy Muhammad Amin Muhammad Faizi, Muhammad Muhammad Hamdan Murbani, Indri Dwi MUSLIMIN IBRAHIM Nailufa, Yuyun Nasronudin Nasronudin Ni Made Mertaniasih Nur Rochmah, Nur Palupi, Dian Eka Roro Poernomo Boedi Setiawan Prabowo, Gwenny Ichsan Priyo Budi Purwono Putri, Hanif Rafika Qurnianingsih, Ema Raharjo Raharjo Rahmawati, Siti Irma Rantam F A Retno Handajani Ridhwan Biosia Wiratama Rini Devijanti Ridwan Ruslan, S. Eriaty N. Saifullah Saifullah Samsriyaningsih Handayani Setiobudi, Karen Delicia Setyawati, Dewi Sidarningsih Sidarningsih, Sidarningsih Sifak Indana Siti Khaerunnisa Sufiyah, Sufiyah Sulistyorini, Anik Susi Wahyuning Asih Susi Wahyuning Asih, Susi Wahyuning Suwandito Suwandito Suwandito, Suwandito Syafa'ah, Irmi Syafa’ah, Irmi TAKAKO UTSUMI Titut Harnanik Tjandrakirana Tjandrakirana Tuti Kusumaningsih Ulfa Kholili Ummah, Fithriyah Cholifatul Ummi Maimunah W Widia Wahyudi, Lindung Wasis Wasis Waskito, Langgeng Agung WULIJARNI, N. WULUARNI, N. Wungu, Citrawati Dyah Kencono Wungu, Citrawati Dyah Kencono Yasan, Yanna Debby Restifanny YOSHIHIKO YANO YOSHITAKE HAYASHI Yudhi Kurniawan Yudi Her Oktaviono, Yudi Her Yulia Ismail Yulia Sari Ismail Zuhrotul Eka Yulis Anggraeni Zulka, Ayesie Natasha

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