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CHARACTERIZATION OF ENVELOPE E PROTEIN OF DENGUE VIRUS INDNESIA ISOLATE Rantam F A; Soetjipto Soetjipto; Dachlan Y P
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 5 No 2 (2000): June 2000
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/472

Abstract

Dengue virus is a human pathogen that has as an increasingly important public health threat. These virus have four serotypes (DEN-1, DEN-2, DEN-3, DEN-4). There is no specific treatment for any pathogenic dengue virus and there are few vaccines available. This study was carry out to E protein characterization from dengue virus infected C5/36 cells for vaccine candidate. We found that E protein induced antibody response. The data of the reactivity on the nitrocellulose membrane between E protein against polyclonal and monoclonal antibodies on western blot analysis, shown that the E protein whisc was once of dengue virus protein has immunogenic. The Indonesia isolate had no difference with the Phillipine isolate. The result suggested that all of the E protein dengue virus strain have peptides overlapping. Also promising for he production of immunogenic particulate proteins, especially DEN protein for the four strains.
Sleep Disorders in Late-Life Depression Katharina Merry Apriliani; dr. Soetjipto
Jurnal Psikiatri Surabaya Vol. 9 No. 1 (2020): May
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (222.289 KB) | DOI: 10.20473/jps.v9i1.16026

Abstract

Depression is an important mental health problem in developed societies, and as many as 50%-90% of patients with depression complain of sleep disorders. In the elderly, sleep disorders comorbid depressive disorders are getting worse. Late-life Depression (LLD) is often not detected because the symptoms are less specific than younger age. It even provides an independent risk factor impact on suicide ideas and behaviour. Causative risk factors of sleep disorders in Late-life Depression are biological and non-biological factors that form the basic of pharmacological and non- pharmacological management.
Frotteuristic Disorders Ayu Nuzulia Putri; Soetjipto Soetjipto
Jurnal Psikiatri Surabaya Vol. 9 No. 2 (2020): November
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jps.v9i2.20086

Abstract

Frotteuristic disorders is a rare paraphilia among other paraphilia. Frotteuristic are usually acquired with other paraphilia such as exhibitionism. Frotteuristic disorders is rubbing the genital area on other people to cause orgasm. This action often occurs in public area such as subways, elevators or shopping centers where victims are unaware. Some studies have difficulty getting data on frotteuristic disorders because this sexual harassment rarely reported to the police because there are no witnesses or evidence. This literature reviews aims to determine the comprehensive treatment for frotteuristic disorders.
Clinical and Demographic Characteristics Differences between M. tuberculosis and M. bovis Infection in Bronchoalveolar Lavage Pulmonary TB Patients, Indonesia Budi Yanti; Soetjipto Soetjipto; Ni Made Mertaniasih; Muhammad Amin
Jurnal Respirologi Indonesia Vol 39, No 4 (2019)
Publisher : Perhimpunan Dokter Paru Indonesia (PDPI)/The Indonesian Society of Respirology (ISR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36497/jri.v39i4.76

Abstract

Background: Some species of the Mycobacterium tuberculosis complex (MTBC) which can cause tuberculosis, particularly M. tuberculosis and M. bovis, may have different virulence property and therefore associated with various clinical severity in tuberculosis (TB) patients. The aim of this study was to determine the differences in clinical and demographic characteristics between M. tuberculosis and M. bovis infection among Indonesian TB patients. Methods: Thirty-one new and active TB patients were confirmed to have acid fast bacilli (AFB) sputum positive and/or Xpert MTB/RIF positive for M. tuberculosis from Dr. Soewandhie Hospital, Surabaya, Indonesia. Interviews were conducted to record the clinical and demographics required. The MTBC were isolated from bronchoalveolar lavage fluid (BALF) and determined by primer-specific PCR targeting TbD1 and RD9 region gene. The degree of lung tissue damage was classified using NICE Scoring System. Results: The MTBC were detected in all patients on whom 19 and 12 isolates were classified as M. tuberculosis and M. bovis respectively. There was a different on 74.2% of productive age subjects (21-50 years) with M. tuberculosis infection (P
Malonaldehyde Level of Administration Ethanol Extract of Purple Sweet Potato Var. Ayamurasaki in Doca-Salt Hypertensive Rats Irma Sarita Rahmawati; Soetjipto Soetjipto; Annis Catur Adi; Aulanni’am Aulanni’am
Journal of Applied Food Technology Vol 5, No 1 (2018)
Publisher : Dept. Food Technology, Faculty of Animal and Agricultural Sciences, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2526.489 KB) | DOI: 10.17728/jaft.56

Abstract

There is an increasing amount of evidence that oxidative stress related to hypertension can damage the function of diverse structures such as aorta. It is a well-established fact that chlorogenic acid and anthocyanine found in purple sweet potato generates bioactive compound with antihypertensive and antioxidant activities. The present study sought to investigate antioxidant activity of extract ethanol of purple sweet potato (EP) in deoxycorticosterone acetate (DOCA–salt)–induced hypertensive rats (Rattus norvegicus). The rats were orally administrated a 95% ethanol extract of purple sweet potato (var. Ayamurasaki) (EP) in a daily dose of 200 and 400 mg/kg body weight for 4 weeks. Aorta total malondialdehyde (MDA) and histopathology of aorta abdominal were examined. Aorta injury was observed in DOCA-salt hypertensive group rats compared to normotensive group rats, as aorta MDA significantly increased  (P <0.05). In contrast, treatment of DOCA-salt hypertensive rats with different dose of EP significantly reduced the total aorta MDA, as well as repair kidney damage, suppressed smooth muscle cell proliferation and lessen aorta wall thickening compared to controls.   This is the first report that demonstrated blood pressure lowering and antioxidative effects of an ethanol extract of purple sweet potato, containing chlorogenic acid, in a DOCA–salt model of hypertension.
HIV Genotype Analysis from HIV Infected Patients in East Java Area Yulia Sari Ismail; Soetjipto Soetjipto; Eddy Bagus Wasito; Nasronudin Nasronudin
Jurnal Natural Volume 12, Number 2, September 2012
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.358 KB)

Abstract

Human Immunodeficiency Virus type 1 (HIV-1) has been known to cause Acquired Immune Deficiency Syndrome (AIDS) disease and has been alaso divided into several subtypes (A, B, C, D, F, G, H, J, K) and Circulating Recombinant Form (CRF). Different characteristics of subtype of the virus and its interaction with host can affect the severity of the disease. This study was aimed to analyze HIV-1 genotypes circulating in HIV/AIDS patients from the East Java region descriptively. Information from this research was expected to complement the data of mocular epidemiology of HIV in Indonesia.  This study used blood plasma from patients who had been tested to be HIV positive who were seeking treatment or are reffered to the Intermediate Care Unit of Infectious Disease (UPIPI) Dr. Soetomo Hospital Surabaya from various area representing the East Java regions. Plasma was separated from blood samples by centrifugation for use in the the molecular biology examination including RNA extraction, nested PCR using specific primer for HIV gp120 env gene region, DNA purifying, DNA sequencing, and homology and phylogenetic analysis. Based on the nucleotide sequence of the HIV gp120 env gene, it was found that the most dominant genotypes in East Java belonged to one group of Circulating Recombinant Form (CRF), namely CRF01_AE and CRF3x_01B, which has been also found in Southeast Asia. In the phylogenetic tree, most of HIV samples (30 samples) were in the same branch with CRF01_AE and CRF3x_01B, except one sample (HIV40) was in the same branch with subtype B.
Anosmia, Dysgeusia, and Comorbidity in COVID-19 Patients with Respiratory Tract Manifestations Jason Lefranda Jocelino Siswanto; Viskasari Pintoko Kalanjati; Soetjipto Soetjipto; Azlin Muhammad Mohd Yasin
Jurnal Respirasi Vol. 9 No. 3 (2023): September 2023
Publisher : Faculty of Medicine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jr.v9-I.3.2023.188-193

Abstract

Introduction: Olfactory and special sensory disturbances such as anosmia and dysgeusia have been found among COVID-19 patients. These manifestations may cause post-covid sequelae, disturbing patients' quality of life who have recovered from COVID-19. This study aimed to analyze the association between anosmia and dysgeusia with the comorbidities in adult COVID-19 patients admitted to Dr. Soetomo General Hospital, Surabaya, from 1 January 2020 to 30 September 2021. Methods: This was a cross-sectional retrospective analysis study based on the medical records of all patients who met the criteria (n = 158). The minimal age inclusion was 18 years old, and the patient must be positively diagnosed with COVID-19 (reverse transcription polymerase chain reaction/RT-PCR). Results: Out of 158 patients, all had a fever, and approximately 93% of patients showed respiratory signs and symptoms. There were 128 patients (81%) who suffered from anosmia, and 15 patients (9.5%) had dysgeusia. Significant associations were found between anosmia with dysgeusia (p = 0.049) and between anosmia with cough (p = 0.003) and dyspnea (p = 0.000). The predominant comorbidities reported were overweight (60%), hypertension (42%), and type-2 diabetes mellitus (37%). Conclusion: There were significant associations between anosmia with dysgeusia and the respiratory tract signs and symptoms instead of the comorbidities reported.
Gambaran Burnout Akademik Mahasiswa Kedokteran Universitas Airlangga Angkatan 2021 Qurrota A&#039;yunin; Widati Fatmaningrum; Soetjipto Soetjipto
Jurnal Ilmiah Universitas Batanghari Jambi Vol 23, No 3 (2023): Oktober
Publisher : Universitas Batanghari Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33087/jiubj.v23i3.4096

Abstract

A heavy academic burden puts medical students at a higher risk of experiencing academic burnout when compared to the general population. Academic burnout is a term used to describe emotional reactions in the form of fatigue, depersonalization, and decreased self-efficacy in students. This study aims to determine the description of academic burnout in Airlangga University medical students class of 2021. This study used an observational analytic study design with a cross-sectional research design to determine the description of academic burnout in medical study program students. This research was conducted at the Faculty of Medicine, Airlangga University in February–May 2023. Measurement of the level of academic burnout in students was carried out using an online school burnout inventory questionnaire via gform. Respondents who took part in the study amounted to 93 respondents with the majority of respondents 60 students (64.5%) were female and 33 students (35.5%) were male, the age of the respondents was 17-21 years. The most common category of academic burnout found in low category of academic burnout represented by 30 students (32.3%), 8 students (8.6%) with very low academic burnout, 28 students (30.1%) with medium academic burnout, 22 students (23.7%) with high academic burnout, and 5 students (5.4%) with very high academic burnout.
ANALYSIS OF HIV SUBTYPES AND CLINICAL STAGING OF HIV DISEASE/AIDS IN EAST JAVA Ismail, Yulia; Soetjipto, Soetjipto; Wasito, Eddy; Nasronudin, Nasronudin
Indonesian Journal of Tropical and Infectious Disease Vol. 3 No. 2 (2012)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (140.202 KB) | DOI: 10.20473/ijtid.v3i2.204

Abstract

Human Immunodeficiency Virus type 1 (HIV-1) known to cause Acquired Immune Deficiency Syndrome (AIDS) disease are divided into several subtypes (A, B, C, D, F, G, H, J, K) and Circulating Recombinant Form (CRF). Different characteristics of subtype of the virus and its interaction with the host can affect the severity of the disease. This study was to analyze HIV-1 subtypes circulatingin HIV/AIDS patients from the East Java region descriptively and to analyze its relationship with clinical stadiums of HIV/AIDS. Information from this research was expected to complement the data of mocular epidemiology of HIV in Indonesia. This study utilited blood plasma from patients who had been tested to be HIV positive who sected treatment to or were reffered to the Intermediate Care Unit of Infectious Disease (UPIPI) Dr. Soetomo Hospital Surabaya from various area representing the East Java regions. Plasma was separated from blood samples by centrifugation for use in the the molecular biology examination including RNA extraction, nested PCR using specific primer for HIV gp120 env gene region, DNA purifying, DNA sequencing, and homology and phylogenetic analysis. Based on the nucleotide sequence of the HIV gp120 env gene, it was found that the most dominant subtypes in East Java were in one group of Circulating Recombinant Form (CRF) that is CRF01_AE, CRF33_01B and CRF34_01B which was also found in Southeast Asia. In the phylogenetic tree, most of HIV samples (30 samples) are in the same branch with CRF01_AE, CRF33_01B and CRF34_01B, except for one sample (HIV40) which is in the same branch with subtype B. HIV subtypes are associated with clinical stadiums (disease severity) since samples from different stages of HIV disease have the same subtype.
DETECTION OF TUMOR NECROSIS FACTOR- (TNF- ) GENE PROMOTERS POLYMORPHISM AMONG LIVER CIRRHOSIS PATIENTS WITH CHRONIC HEPATITIS B VIRUS (HBV) INFECTION IN SURABAYA, INDONESIA Wungu, Citrawati Dyah Kencono; Amin, Mochamad; Ruslan, S. Eriaty N.; Purwono, Priyo Budi; Kholili, Ulfa; Maimunah, Ummi; Setiawan, Poernomo Boedi; Lusida, Maria Inge; Soetjipto, Soetjipto; Handajani, Retno
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 5 (2019)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1302.693 KB) | DOI: 10.20473/ijtid.v7i5.7275

Abstract

Polymorphisms in TNF-α gene promoter region are known of its role in the production of TNF-α which may influences the pathogenesis of liver disease. SNPs in positions 238 and 308 of TNF-α gene promoters may affect the production of these cytokines. This study was aimed to detect Single Nucleotide Polymorphism (SNP) on -238 and -308 positions in the TNF-α gene promoter among liver cirrhosis patients with HBV infection in Surabaya, Indonesia. This was descriptive exploratory research with cross sectional study design using serum liver cirrhosis patients with HBV infection in Endoscopy Outpatient Clinic Dr. Soetomo General Hospital, Surabaya from April-May 2017. SNPs at -238 and -308 on TNF-α gene promoter (rs361525 and rs1800629 respectively) were detected using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) with primers specific for the TNF-α promoter region and restriction enzymes NcoI and MspI. The genotypes of TNF-α gene promoter were assessed according to the length of the fragments produced in RFLP. Serum TNF-α levels was measured by commercial ELISA. In this study, as much as 149 positive HBsAg patients was found in Endoscopy Outpatient Clinic, Dr. Soetomo General Hospital, Surabaya. From those amount, as much as 30 liver cirrhosis patients with positive HBsAg were obtained. From 2/30 (6.7%) patients showed the GA heterozygote SNP either position -238 or -308. No patient had the AA genotype. Median blood TNF-α level in women (38 ng / L) was higher than in men (33 ng / L). TNF-α levels in patients with GA heterozygote genotype at -238 and -308 in this research was not different than wild-type (GG genotype). Among patients with liver cirrhosis due to chronic HBV infection in Surabaya, Indonesia, Surabaya, we found GA polymorphisms the TNF-α promoter gene at positions -238 and -308 in 6.7% patients, and did not find homozygous AA polymorphisms. Further studies including larger numbers of patients from various ethnic backgrounds in Indonesia are needed to provide robust data on TNF-α gene promoter polymorphisms and their role in the pathogenesis of liver cirrhosis with HBV infection in this country.
Co-Authors Abdurachman Abdurachman Adi, Ginanjar Sasmito Aksono HP., Eduardus Bimo Amin, Indah Binti Mohd Amin, Mochammad Aminuyati Anang Endaryanto Anggraeni, Zuhrotul Eka Yulis Annis Catur Adi Annisa Rizky Maulidiana Arumisore, Netty M Asnita, Asnita Aulanni'am, Aulanni'am Aulanni’am Aulanni’am Ayesie Natasha Zulka Ayu Nuzulia Putri Azlin Muhammad Mohd Yasin Bakhtiar, Arief Bayu Satria Wiratama Budi Yanti Chorisma Permata Putri Nurazizah Citriana, Mirna Yunik Dachlan Y P Damba Bestari Dienanta, Savira Eddy Bagus Wasito Eddy Wasito, Eddy Elvaretta, Clarissa Ely Rahmatika Nugrahani Ely Rahmatika Nugrahani, Ely Rahmatika Era Catur Prasetya Fedik Abdul Rantam Fitria Sarnita G. Panggabean Ginanjar Sasmito Adi HAK HOTTA HANSON, J. Harianto Notopuro Hendri Susilo Hendy Hendarto Hermanto, Fery Herniyati HERNOMO ONTOSENO KUSUMOBROTO Huda, Khusnul Humairah, Ira Ida Bagus Narmada Indri Safitri Indri Safitri, Indri Jason Lefranda Jocelino Siswanto Juminten Saimin Juniastuti Juniastuti Katharina Merry Apriliani Khaerunnisa, Siti Kirana, Tjandra Latifatu Choirunisa Lukitasari, Lina Luqman Hakim Andira M. THAMRIN HIDAYAT Machin, Abdulloh Maghfiroh, Faridatul Marcos, Imella MARIA INGE LUSIDA Maria Inge Lusida MOCHAMAD AMIN Mufiannoor, Eddy Muhammad Amin Muhammad Faizi Muhammad Hamdan Murbani, Indri Dwi MUSLIMIN IBRAHIM Nailufa, Yuyun Nasronudin Nasronudin Ni Made Mertaniasih Nur Rochmah Nur Rochmah, Nur Palupi, Dian Eka Roro Poernomo Boedi Setiawan Prabowo, Gwenny Ichsan Priyo Budi Purwono Putri, Hanif Rafika Qurnianingsih, Ema Qurrota A&#039;yunin Raharjo Raharjo Rahmawati, Siti Irma Rantam F A Retno Handajani Ridhwan Biosia Wiratama Rini Devijanti Ridwan Ruslan, S. Eriaty N. Saifullah Saifullah Samsriyaningsih Handayani Setiobudi, Karen Delicia Setyawati, Dewi Sidarningsih Sidarningsih, Sidarningsih Sifak Indana Siti Khaerunnisa Sufiyah, Sufiyah Sulistyorini, Anik Susi Wahyuning Asih Susi Wahyuning Asih, Susi Wahyuning Suwandito Suwandito Suwandito, Suwandito Syafa'ah, Irmi Syafa’ah, Irmi TAKAKO UTSUMI Titut Harnanik Tjandrakirana Tjandrakirana Tuti Kusumaningsih Ulfa Kholili Ummah, Fithriyah Cholifatul Ummi Maimunah Viskasari P. Kalanjati W Widia Wahyudi, Lindung Wasis Wasis Waskito, Langgeng Agung Widati Fatmaningrum WULIJARNI, N. WULUARNI, N. Wungu, Citrawati Dyah Kencono Yasan, Yanna Debby Restifanny YOSHIHIKO YANO YOSHITAKE HAYASHI Yudhi Kurniawan Yudi Her Oktaviono, Yudi Her Yulia Ismail Yulia Sari Ismail Zuhrotul Eka Yulis Anggraeni Zulka, Ayesie Natasha