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All Journal Dinamik INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES Jurnal Ilmu Lingkungan Jurnal Ilmu Kedokteran (Journal of Medical Science) Jurnal Ners Indonesia Jurnal Kedokteran Syiah Kuala Jurnal Kedokteran Brawijaya Majalah Kedokteran Bandung Jurnal Teknik Mesin Medical Journal of Indonesia Jurnal Online Mahasiswa (JOM) Bidang Kedokteran Jurnal Kesehatan Masyarakat Andalas (Andalas Journal of Public Health) Berkala Kedokteran Jurnal Kesehatan Melayu Jurnal Kesehatan Komunitas Jurnal Kesehatan Manarang Riau Journal of Empowerment JURNAL PHOTON Jurnal Kreativitas PKM Ensiklopedia of Journal PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Majalah Kedokteran Andalas Al-Tamimi Kesmas: Jurnal Ilmu Kesehatan Masyarakat (Journal of Public Health Sciences) Jurnal Mekanova : Mekanikal, Inovasi dan Teknologi Jurnal Informasi dan Teknologi KAIBON ABHINAYA : JURNAL PENGABDIAN MASYARAKAT PREPOTIF : Jurnal Kesehatan Masyarakat Jurnal Pengabdian Masyarakat: Darma Bakti Teuku Umar Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences Journal of Computer Networks, Architecture and High Performance Computing Al-Iqra Medical Journal : Jurnal Berkala Ilmiah Kedokteran Jurnal Kesehatan Tambusai Jurnal Zona Narra J Malikussaleh Journal of Mechanical Science Technology Dinamika Lingkungan Indonesia Prosiding Konferensi Nasional PKM-CSR SIGMA: Information Technology Journal JKKI : Jurnal Kedokteran dan Kesehatan Indonesia eJournal Kedokteran Indonesia Malcom: Indonesian Journal of Machine Learning and Computer Science JIF EcoNews Riset Informasi Kesehatan Jurnal Polimesin Proceeding International Conference on Education
Dedi Afandi
Universitas Riau
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Effect of pH and neutrophil count on the motility and persistence of spermatozoa in the vagina of candidiasis rat models Iswara, Raja AW.; Hestiantoro, Andon; Budiningsih, Yuli; Werdhani, Retno A.; Birowo, Ponco; Wuyung, Puspita E.; Afandi, Dedi
Narra J Vol. 4 No. 2 (2024): August 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i2.823

Abstract

Sexual violence is a global issue affecting individuals regardless of their relationship to the perpetrator or the setting. Microscopic examination of spermatozoa from vaginal swabs is crucial in investigating cases of sexual intercourse to determine the time of the crime. Factors such as vaginal pH and neutrophil count influence the motility and persistence of spermatozoa in the vagina, particularly in conditions like candidiasis, highlighting the need for further research in this area. This study aimed to determine the effect of pH and neutrophil count on the motility and persistence of spermatozoa in the vagina with candidiasis. An experimental study was conducted using white rats (Rattus norvegicus) of the Wistar strain, with four male rats providing spermatozoa samples and 32 female rats receiving treatment. The female rats were divided into two groups: the normal group and the candidiasis model group. In both groups, the female rats were given vaginal insemination of spermatozoa. Variables measured included pH, neutrophil count, motility, and persistence of spermatozoa in the vagina. Data were analyzed using the Mann-Whitney test, followed by the Spearman correlation test. The findings revealed that spermatozoa motility lasted up to three minutes in normal rats, whereas in the candidiasis model, it was reduced to two minutes. Additionally, spermatozoa persistence in the vagina lasted up to six days in the normal group compared to up to three days in the candidiasis model. There were significant differences in pH, neutrophil count, motility, and persistence of spermatozoa in the vagina between the normal group and the candidiasis model (all had p<0.001). There was a correlation between pH and neutrophil count with the motility and persistence of spermatozoa in the rat’s vagina (p<0.001). In conclusion, vaginal pH and neutrophil count influence the motility and persistence of spermatozoa in the vagina of candidiasis rat models.
Impact of semen insemination on the vaginal microbiome profile of candidiasis rat model: A preliminary forensic study on sexual violence evidence Iswara, Raja AFW.; Hestiantoro, Andon; Budiningsih, Yuli; Werdhani, Retno A.; Birowo, Ponco; Wuyung, Puspita E.; Fadilah, Fadilah; Afandi, Dedi
Narra J Vol. 5 No. 1 (2025): April 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i1.1256

Abstract

Sexual violence, including sexual intercourse, can occur in women experiencing vaginal discharge, particularly in cases of vaginal candidiasis. In candidiasis, the vaginal microbiome undergoes changes that could serve as a diagnostic indicator or as evidence of sexual activity.  The aim of this study was to assess the effects of semen insemination on the vaginal microbiome profile of candidiasis rats and to determine its forensic investigations in cases of sexual violence.  An experimental study was carried out using Wistar strain rats (Rattus norvegicus), consisting of four male rats (for spermatozoa donors) and twenty-four female rats. The female rats were divided into four groups: normal condition (control), normal condition post-semen insemination, candidiasis rats, and candidiasis rat post-semen insemination. Vaginal microbiome profiles were examined for each group, using alpha diversity (Chao 1, Shannon, Simpson, and Faith PD indices) and beta diversity (Bray Curtis, Jaccard, Unweighted Unifrac and Weighted Unifrac indices). Data were analyzed using the Kruskal-Wallis test for alpha diversity and the PERMANOVA test for beta diversity. The vaginal microbiome profiles of normal and candidiasis rats showed no significant differences (p>0.05). In candidiasis rats, the microbiome predominantly consisted of the Eukaryota kingdom, particularly Candida albicans. Semen insemination did not significantly affect the vaginal microbiome profile of candidiasis rats in the short term (p>0.05). However, the study highlights that the presence of Eschericia-Shigella, Roseomonas, and Archaea in the vaginal microbiome post-semen insemination potentially serves as an indicator of infection or sexual activity in forensic contexts.
Co-Authors ', B. Isyandi ' - Thamrin - Thamrin . Zulfan A.A. Ketut Agung Cahyawan W Abdurrahman Hamid Achsanul Kubri Adhistie Indah Sari Adnan Kasry Agrina Agrina Agus Purwadianto Ahmad Hanafi Ahmad Hanafi Ahmad Syuhada Alida Widiawaty Ananto Tri Sasongko Andhika, Kevin Rovi Andon Hestiantoro Anggraini, Shella Annisa Abdi Ghifari Antika Zahrotul Kamalia Aos Kuswandi Apriani Dewi Aprilliantoni Aras Mulyadi Arfianti Arfianti Aria Gusti Arini, Merita Ariza Julia Paulina Arni Amir Asbar Asbar, Asbar Asfeni, Asfeni Asiyah Uswatun Nisa Asnawi Aufan Rizmuafa Azzahra, Puan Justitia B Isyandi Bachtiar, Dandi Bambang Budi Siswanto BDM, Nurfifi Pratiwi Bernard Isyandi Bhelprinosa Arwen Bintal Amin Budi Hartono Budi Hartono Budi Sampurna Chunin Widyaningsih D, Fauzia Andrini Damayanti, Elok Damri Damri Dani Rosdiana Danisha, Dinda Darmawan, Daniel Darwin Darwin Deby Andita Dendy K. Pramudito Desby Juananda Dessyka Febria, Dessyka Dhea Adellina Riyadi Dhea Adellina Riyadi Dian Rahmi Yenti Dicky Pangestu Sandjaya Dwi Novitasari Dzakwan, M. Alif Efriyeldi, Efriyeldi Eka Sri Indra Putri Elnovrian Purnama Saghita Elva Susanty Endang Ratnaningsih Ennimay, Ennimay Erika Erika Eriyan, Muhammad Firdaus Fadilah Fadilah, Fadilah Fadlan Tri Ramadhan Fatah Abdul Khair fatayat, Nurul Fetty Try Rahmadani Fifia Chandra Fifia Chandra Fika, Rana Citra Fikri Ruriandy Aufi Fimadani, Ersa Fitra, Julia Fitriani, Ernita Gurning, Riris Naomi Hadapi Akhbar, Ahmad Hananto Andriantoro Hari Utomo Harima Gumanty, Harima Herdianto Herdianto Herkutanto Herkutanto Herniwanti Herniwanti Hetty Hirfawaty, Hetty Hetty Ismainar Hillbertina, Noza Huriatul Masdar Idrus Alwi Ilhami Romus Indi Esha Indra Fakhreza Indrayana, Mohammad T. Inge Sutanto Intan Rosaline Simangunsong Irwansyah Irwansyah Isarianto Iswara, Raja AFW. Iswara, Raja AW. Ivan R. Widjaja Ivan Riyanto J. W. Marwoto Jefryanto " Jepisah, Doni Jimmi Copriady Kasendra, Mukhamad Khodijah Khodijah Kholida Hosni Komala Sari Kurniawan Kurniawan Laode Burhannudin Mursali Laode Burhannudin Mursali, Laode Burhannudin Laode Burhanuddin Laode Burhanuddin Mursa Laode Burhauddin Mursali Lilly Haslinda Lita Lita Lukman H. Makmun M.Farhan Akhyar M.Randy Kurniadi M.Teguh Hutomo Mandatasari, Melissa Marlina, Hastuti Marzuki, Nurhidayati Maysa, Hana Maysarah Elnas Media, Yofiza Merita Arini Mirna Ilza Mislindawati Mislindawati Mislindawati Mitra Mitra Mohammad Tegar Indrayana Mohammad Tegar Indrayana Mohammad Tegar Indrayana Mohammad Tegar Indrayana Mokhammad Raihan Eka Putra Muhammad Ilham Maulana Muhammad Maliki Muhammad Nuzan Rizki Muhammad Yulis Hamidy Novita Rani Nugraha, Iriandanu Nur Fitri Fadila nur helmi Nur Hidayah Nurhapipa, Nurhapipa Nurhay Abdurachman Nurjati Chairani Nurul Islami, Nurul Nuzulia Irawati Oktavia Dewi, Oktavia Oktavinda Safitry Olvhantiara Sukma Paramita, Ranika Ponco Birowo Popi Novia Puspita E. Wuyung Putri, Rintan Dwi Rahayu, Endang Purnawati Rahmat Azhari Kemal Rany, Novita Rasoel Hamidy Ratih Wirda Ningsih Ratna Sary Ratna Sary Rawina W Razali Razali Retno A. Werdhani Ricky Rusydi Satriawan Ridha Restila Ridho Maulana Rieskariesha Kiswara Rifardi, Dhandia Rika Susanti Rima Ayu Lestari Riswandi " Rizki Aulia Nanda Roziah Roziah Rozikin, Zaenur S.Pd. M Kes I Ketut Sudiana . Saghita, Elnovrian Purnama Sarah Miftahul Rahmi Sarwo Edhy S Satrio Mandala Tinardy Serly Marici Silaban, Helen Ernawati Siregar, Fajri Marindra Siska Mayang Sari Siti Aisyah Sitti Zuhairah Sri Intan Agusmai Sri Nanda Ayusa Sri Wahyuni Stella Putri Wanda Suci Maya Sari Suderajat, Agung Sukendi Sukendi Sulaeman, Asep Arwan Sunki Yulia Dwi P Suri D. Lesmana Suri Dwi Lesmana Suryani Suryani Sutisna Himawan Suyanto " Suyanto Suyanto Syahputra, Roni Eka SYAIFUL BAHRI Syarifah Hidayah Fatriah T.Resty Yulandari Talitha Lailatul Hafizah Taufik Sofistiawan Tia Harjianti Tiara Anggraini Tika Mutia Tuti Restuastuti Tyas Handayani Uning Pratimaratri Vani Ardiani Venni Oktary Amir Viktor Amrifo Wahyu Setiawan Wilda Septi Pratiwi Winda Kristanti Wiwit Ade Fidiawati Yanwirasti Yanwirasti Yepi Kurniadi Yessi Harnani Yoga Yuniadi Yolanda Nurliza Yolazenia Yolazenia Yose Rizal Yufandi, Asra Yuli Budiningsih Yulianto, Ragil Yuliati Yuliati Yullya Tri Utari Yunita, Jasrida Yusni Ikhwan Siregar Zarfiardy Aksa Fauzi Zulfan Saam Zulfan Saam Zulharman " Zulharman Zulharman Zurrahmi Z.R