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A REVIEW: MICROPROPAGATION OF PHALAENOPSIS sp FROM LEAF AND FLOWER STALK EXPLANTS Meutia Zahara
Jurnal Natural Volume 17, Number 2, September 2017
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v17i2.8130

Abstract

Abstract Phalaenopsis orchids are recognized as the most popular orchid genus in the world, especially in horticultural industry due to their large, colorful, and durable flowers as well as their wider adaptability to room conditions. The characteristics of seedling propagated by vegetative means are not uniform; therefore, propagation through tissue culture is desirable. Although the micro propagation of Phalaenopsis has shown very good development, but the wide spread of micro propagation still limited due some problems such as the exudation of phenolic compounds, the PGR concentration, the media used, somaclonal variation, the chosen explants, etc. This paper endeavor to include some important investigations based on the common explants used; leaf and flower stalk. Keywords: Micropropagation, Phalaenopsis, leaf explant, flower stalk ReferencesAnonymous. Orchid (Orchidaceae). Diakes tanggal 13 Januari 2013 dari http://www.rainforest-alliance.org/kids/species-profiles/orchid. Rainforest Alliance. 2002.Pillon, Y.; Chase, M. W.Taxonomic exaggeration and its effects on orchid conservation. Conservation Biology. 2007, 21, 263–265.Thengane, S. R.; Deodhar, S. R.; Bhosle, S. V.; Rawal, S. K. Direct somatic embryogenesis and plant regenaration in Garciniaindica Chois’. Current Science. 2006, 91(8), 1074-1078.Yuswanti, H.; Dharma, I. P.; Utama. ; Wiraatmaja, I. W. Mikropropagasi anggrek Phalaenopsis dengan menggunakan eksplan tangkai bunga. AGROTROP. 2015, 5(2): 161-166.Raynalta, E.; Sukma, D.  Pengaruh komposisi media dalam perbanyakan protocorm like bodies, pertumbuhan plantlet, dan aklimatisasi Phalaenopsis amabilis. J. Hort. Indonesia. 2013, 4(3): 131-139.Kosir, P.; Skof, S.; Luthar, Z. Direct Shoot Regeneration from Nodes of Phalaenopsis of Orchids. Acta Agriculturae Slovenica. 2004, 83, 233–242.Arditti, J. R. ; Ernst. Micropropagation of Orchids. Wiley-Interscience. New York, 1993.Park, Y. S.;Kakuta, S.; Kano, A.; Okabe, M.Efficient propagation of protocorm-like bodies of Phalaenopsis in liquid medium. Plant Cell, Tissue and Organ Culture. 1996, 45, 79–85.Park, S. Y. ; Yeung, E. C.; Chakrabarty, D. ; Paek, K. Y. An efficient direct induction of protocorm-like bodies from leaf subepidermal cells of Doritaenopsis hybrid using thin-section culture. Plant Cell Reports. 2002, 21, 46–51.Zahara, M.; Datta, A.; Boonkorkaew, P. Effects of sucrose, carrot juice and culture media on growth and net CO2 exchange rate in Phalaenopsis hybrid ‘Pink’. ScientiaHorticulturae. 2016,205, 17–24.Hee, K. H.; Loh, C. S.; Yeoh, H. H. In vitro flowering and rapid in vitro embryo production in Dendrobium Chao Praya Smile (Orchidaceae). Plant Cell Reports. 2007, 26, 2055–2062.Kannan, N. An in vitro study on micropropagation of Cymbidium orchids. Current Biotica. 2009, 3, 244–250.Steward, Jr. N. C. Plant Biotechnology and Genetics. Willey, A john Willey Sons, INC., Publication. 2008.George, E. F.; Sherington, P. D.Biotechnology by tissue culture. Exegetics Ltd. 1994.Nursyamsi. Teknik kultur jaringan sebagai alternatif perbanyakan tanaman untuk mendukung rehabilitasi lahan. Makalah pada ekspose hasil-hasil penelitian balai penelitian kehutanan makasar. Makasar, 2010.Aditi, J. F. L. S.; Krikorian, A. D. Orchid mircropropagation: the path from laboratory to commercialization and an account of several unappreciated investigators. Botanical Journal of of the Linnean Society. 1996, 122: 183-241.Gunawan, L. W. Teknik Kultur Jaringan Tanaman. Pusat Antar Universitas (PAU) Bioteknologi IPB. 1998. Bogor.Chugh, S. Guha, S.; Rao, I. U. Micropropagation of orchids: A review on the potential of different explants. Scientia Horticulturae. 2009, 122, 507–520.Ramdan. Kultur daun dan pangkal batang in vitro anggrek bulan raksasa (Phalaenopsis gigantea J.J.Smith) pada beberapa media kultur jaringan. Departemen agronomi dan hortikultura, Fakultas pertanian IPB. 2011.Latip, M. A. R.; Murdad, Z. A.; Aziz, L. H.; Ting, L. M.; Govindasamy.; R. Pipin. Effects of N6-Benzyladenine and Thidiazuron on Poliferation of Phalaenopsis gigantea Protocorm. AsPac J. Mol. Biol. Biotechnol. 2010, 18(1): 217-220 p.Niknejad, A.; Kadir, M. A.; Kadzimin, B. S. In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroidaceae: Orchidaceae). African Journal of Biotechnology.2010, 10, 11808–11816.Chen, J. T.; Chang, W. C. Direct somatic embryogenesis and plant regeneration from leaf explants of Phalaenopsis amabilis. Biologia Plantarum. 2006, 50, 169–173.Zahara, M. Disertasi doktor: The Effects of Plant Growth Regulators and Natural Additives on Direct Shoot Regeneration and Plantlet Growth of Phalaenopsis hybrid ‘Pink’. Asian Institute of Technology, Pathumthani. Thailand. 2016.Xu, C. J.; Li, H.; Zhang, M. G. Preliminary studies on the elements of browning and the changes in cellular texture of leaf explant browning in Phalaenopsis. Acta Horticulturae Sinica. 2005, 32, 1111–1113.Tokuhara, K; Mii, M. Induction of embryonic callus and cell suspension culture from shoot tips excised from flower stalk buds of Phalaenopsis (Orchidaceae). In Vitro Cellular Developmental Biology–Plant. 2001, 37, 457–461Balilashaki, K.; Naderi, R.; Kalantari, S.; Soorni, A. Mircropropagation of Phalaenopsis amabilis cv Cool ‘Breeze’ with using flower stakl nodes and leaves of sterile obtained from node cultures. IJFAS, 2014.Semiarti, E.; Indrianto, A.; Purwanto, A. Agrobacterium-Mediated transformation of Indonesian orchids for  micropropagation, genetic transformation, Prof. MarÃa Alvarez (Ed.), ISBN: 978-953-307-364-4, InTech, 2011. Available from: http://www.intechopen.com/books/ genetic-transformation/agrobacterium-mediated-transformation-ofindonesian-orchids-for-micropropagation.
PENGARUH URIN SAPI PADA PERTUMBUHAN AKAR STEK KOPI ROBUSTA (Coffea canephora) di ACEH JAYA Masrita, Masrita; Zahara, Meutia
Jurnal Jeumpa Vol 8 No 1 (2021): Jurnal Jeumpa
Publisher : Department of Biology Education, Faculty of Teacher Training and Education, Samudra University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/jj.v8i1.4127

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Penelitian yang dilaksanakan di Ranto Sabon, kecamatan Sampoinet, Aceh jaya pada bulan April 2019 ini bertujuan untuk melihat pengaruh pemberian urin sapi pada pertumbuhan akar stek kopi Robusta (Coffea canephora). Analisa data menggunakan Rancangan Acak Kelompok (RAL) satu faktor dan empat perlakuan konsentrasi urin sapi 0%, 30%, 50%, dan 70%, diaplikasikan pada akar stek kopi Robusta. Parameter yang diamati berupa jumlah akar, berat akar dan panjang akar. Hasil penelitian menunjukkan bahwa konsentrasi urin 70% memiliki jumlah rata-rata akar terbanyak (19), berat akar rata-rata tertinggi diperoleh pada 0% (1.3 g) urin sapi dan konsentrasi 30% urin sapi menunjukkan rata-rata panjang akar tertinggi (1.93 cm)
Ulasan singkat: Deskripsi Kembang Telang (Clitoria ternatea L.) dan Manfaatnya Zahara, Meutia
Jurnal Jeumpa Vol 9 No 2 (2022): Jurnal Jeumpa
Publisher : Department of Biology Education, Faculty of Teacher Training and Education, Samudra University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/jj.v9i2.6509

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Letak Indonesia baik secara geografis dan iklim sangat cocok untuk ditanam berbagai jenis tanaman yang dapat dimanfaatkan dalam kehidupan sehari-hari. Bunga telang dengan nama ilmiah Clitoria ternatea L. yang tergolong ke dalam famili Fabaceae merupakan salah satu jenis tanaman yang hidup dengan baik di Indonesia. Seluruh bagian tanaman ini dapat dimanfaatkan sebagai bahan pangan dan juga pada bidang kesehatan. Bunga ini sering dimanfaatkan sebagai pewarna makanan dan minuman alami, kandungan fitokimianya yang banyak memberikan manfaat dalam bidang kesehatan seperti antioksidan, antibakteri, anti inflamasi, analgesik, antiparasit, antihistamin, dan meningkatkan sistem imun. Pengumpulan informasi pada publikasi ini menggunakan metode studi pustaka baik secara online maupun offline, diutamakan publikasi yang terbaru kecuali yang berkaitan dengan taksonomi. Beberapa website yang digunakan yaitu google scholar, researchgate, academia, dan scopus. Hasil pencarian informasi menunjukkan bahwa telang memiliki banyak manfaat bagi manusia, diharapkan informasi yang tercantum di dalam ulasan singkat ini dapat menjadi wacana untuk budidaya bunga telang. Ulasan singkat ini berisi beberapa informasi penting tentang botani, fitokimia dan manfaat bunga telang yang dapat menjadi bahan acuan bagi penelitian selanjutnya.
Respon Pertumbuhan Tanaman Sawi (Brassica Juncea L.) dengan Penambahan Arang Sekam pada Media Tanam Serbuk Gergaji Asyifa, Putri; Zahara, Meutia; Kamaliah, Kamaliah
Jurnal Jeumpa Vol 10 No 2 (2023): Jurnal Jeumpa
Publisher : Department of Biology Education, Faculty of Teacher Training and Education, Samudra University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/jj.v10i2.8573

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Penggunaan media tanam seperti tanah yang baik semakin berkurang, maka diperlukan media alternatif berupa serbuk gergaji dan arang sekam yang ketersediaanya tinggi di lingkungan. Penelitian ini bertujuan untuk mengetahui respon pertumbuhan tanaman sawi (Brassica juncea L.) dan perbandingan yang optimum dengan penambahan arang sekam pada media tanam serbuk gergaji. Penelitian ini dilaksanakan di Greenhouse Prodi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Ar-Raniry pada Oktober-November 2022, menggunakan metode kuantitatif dengan rancangan acak lengkap (RAL) non faktorial 4 perlakuan dengan 5 pengulangan, yaitu P0 = serbuk gergaji (1 : 0), P1 = serbuk gergaji + arang sekam (1 : 1), P2 = serbuk gergaji + arang sekam (1 : 2), dan P3 = serbuk gergaji + arang sekam (1 : 3). Analisis data menggunakan one way anova dengan SPSS versi 26. Hasil penelitian menunjukkan bahwa pertumbuhan tanaman sawi menggunakan media tanam serbuk gergaji dan arang sekam tidak berpengaruh nyata terhadap tinggi tanaman, luas daun, jumlah daun, berat basah, dan berat kering tanaman sawi. Namun berpengaruh nyata terhadap kandungan klorofil dan distribusi stomata pada P1 dengan jenis stomata yaitu parasitik. Perbandingan media tanam serbuk gergaji dan arang sekam yang optimum terhadap pertumbuhan tanaman sawi terdapat pada P1.
Identification of Zingiberaceae as medicinal plants in Gunung Cut Village, Aceh Barat Daya, Indonesia Zahara, Meutia; Hasanah, Mauizah; Zalianda, Rahma
Journal of Tropical Horticulture Vol 1, No 1 (2018): October 2018
Publisher : Yayasan Pertanian Tropika Indonesia (YPTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1386.477 KB) | DOI: 10.33089/jthort.v1i1.9

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Zingiberaceae is well known as gingers family with abundant numbers of spices widely used in Southeast Asia, because of their unique flavors, ornamental and also the medicinal values. The extracts of Zingiberaceae rhizome contain many essentials oils, including terpenes, alcohols, ketones, flavonoids and phytoestrogens that use as medicine. Zingiberaceae grow vigorously in a wide range of habitats ranging from riverine to the limestone area, from lowland to the upper mountain regions. They often grow in shady area but some of the native species are able to tolerate to the sun exposure. The cultivation of this family was developed widely. Gunung Cut Village is one the villages in Aceh Barat Daya district which cultivated four species of this family: Zingiber officinale, Curcuma domestica, Alpina galanga and Kaempferia galanga. They mainly use for medicinal purposes as it can cure many diseases traditionally.
A Review: The Effect of Plant Growth Regulators on Micropropagation of Aglaonema sp. Zahara, Meutia; Win, Cho Cho
Journal of Tropical Horticulture Vol 3, No 2 (2020): October 2020
Publisher : Yayasan Pertanian Tropika Indonesia (YPTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33089/jthort.v3i2.58

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Nowadays, Aglaonema sp. becomes very famous in Indonesia. especially during the pandemic covid-19. Many people tend to stay home to stop the spreading of the virus. One of the activities that can be done during staying home is growing the plants as well as ornamental plants. Aglaonema sp. is one of the important and well-known foliage ornamental plants in Indonesia, commonly called “Sri Rezeki”. The generative or sexual reproduction of Aglaonema sp. is difficult, and it contains some endogenous pathogen. The common propagation techniques are by cutting and seed, which risky in the spreading of the pathogen. Micropropagation is an advanced technique to produce a large number of plants in a short time and pathogen-free transplant. This manuscript endeavor to include some important investigations and studies on the use of the Plant Growth Regulators (PGRs) on the Aglaonema sp. micropropagation.
Morphological and Stomatal Characteristics of Two Indonesian Local Orchids Zahara, Meutia; Win, Cho Cho
Journal of Tropical Horticulture Vol 2, No 2 (2019): October 2019
Publisher : Yayasan Pertanian Tropika Indonesia (YPTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1331.022 KB) | DOI: 10.33089/jthort.v2i2.26

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Orchids are very famous for their beautiful and long-lasting flowers and variety among the most diverse family of flowering plants in the world, comprising thousand species and hybrids and very attractive. The research aimed to determine the morphological features of two local orchids Phalaenopsis amabilis L. and Dendrobium x Superbiens and the stomatal characteristics of both orchids. Plants were the only living organisms that are able to convert light energy/sunlight into chemical energy, and stomata is an important part of plants for CO­2 to enter the leaf and H­­2O to be released during the photosynthetic reactions. The results showed that Phalenopsis amabilis L. was monopodial orchid and Dendrobium x Superbiensis a sympodial orchid. A qualitative descriptive research method used to describe and interpreted the type of stomata of Phalaenopsis amabilis L and Dendrobium sp. stomatal density were counted on adaxial and abaxial part of the leaf, and the highest stomatal density was obtained on the abaxial part of Dendrobium x Superbiensleaf 290.81 mm­-2. The type of stomata was anomocytic for two local orchids.
A Brief Review of Efficacious Plants in the World: Tagetes (Marigold) Zahara, Meutia; Arifin, Vera Nazhira; Hamama, Syarifah Farissi
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 2 (2024): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.85079

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Tagetes is a genus consisting of several efficacious plant species known as marigold, which is native to Mexico and has  spread worldwide due to its ability to adapt to different soil conditions. This genus is very popular among gardeners because it’s easy to cultivate, adaptable, produces flowers all year round, has a free flowering habit, short duration, and the flower has an attractive color and shape. It has many flowers of varying colors such as red, orange, yellow, mixed red-orange or red-yellow, but the yellow and orange color are the most popular. The flowers contain many carotenoids, which act as antioxidants, anti-bacterial, anti-inflammatory, anti-carcinogen, nematicide, and cosmetics. The carotenoid can also cure fever, epileptic fits, astringent, scabies, liver complaints, stomachache, sore throat, and  can be used as a natural moisturizer, and natural mosquito repellent Besides its use as a medicinal plant, Tagetes is also used as ornamental plants and edible plants. This manuscript’s endeavour is to include some important investigations and studies about the general description, phytochemicals compounds and essential oils, medicinal uses, natural pesticides, and food and beverage uses of the marigolds. 
THE USE OF PUPPET STORY FOR BASIC ENGLISH INTRODUCTION TO 1ST GRADE ELEMENTARY SCHOOL STUDENTS Meutia Zahara; Rahmatun Nisa; Cut Mawar Helmanda
ABDIMU: Jurnal Pengabdian Muhammadiyah Vol 2, No 1 (2022): Vol 2, No 1, Juni 2022
Publisher : Universitas Muhammadiyah Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37598/abdimu.v2i1.1361

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This community service activity had been held for 1st grade elementary school of SD Muhammadiyah Banda Aceh during June 2022. The use of puppet and storytelling in introducing basic English is an interesting and fun activity for children. This activity aimed to make the participants able to understand basic English quickly which can be used as a stepping stone to understand English daily. The methodology used was the lecture method using puppet in the form of cards that have been attached to ice cream sticks and colored. The materials provided were alphabets, numbers and colors, then followed by storytelling using other puppets entitled The bear and friends. The results obtained that the participants were very enthusiastic and able to catch the material quickly, this can be seen when the participants could answer all the questions about the materials provided.Keyword: Puppet story, basic English, community service
ISLAMIC STUDIES TECHNIQUE THROUGH PLAYING, STORY TELLING AND SINGING METHOD FOR KIDS IN LAMBIRAH VILLAGE ACEH BESAR Meutia Zahara; Nazariah Nazariah; Muhammad Yani; Restu Andrian
ABDIMU: Jurnal Pengabdian Muhammadiyah Vol 1, No 1
Publisher : Universitas Muhammadiyah Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37598/abdimu.v1i1, Juni.1074

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This community service program aims to teach the Islamic studies to the children with more interesting techniques, so that the children will have more understanding to the material given, this program conducted in Lambirah Village. One of well-known techniques that mostly apply is Playing, Singing and Telling method. This program conducted with some stages; providing the materials to the children, followed by some trainings dan discussion with the audience. The results obtained that this method was successfully attracted the audience or participants attention.Key words: Lambirah, Islamic Studies, BCM Method
Co-Authors Abdul Majid Abdurrahman, Faisal Bin Adamy, Aulina Afrida, Maina Aini Qurratu Aini, Qurratu Ajarni Ajarni Alfad Darul Umra Alma Aletta Alma, Siti Amiren, Muslim Anisrina Anisrina Arifin, Vera Nazhira Aripin Ahmad, Aripin Aslam Nur Asnawi Abdullah Asnawi Abdullah Asnawi Abdullah Asyifa, Putri Aulia zulkifli, Riski Baharuddin, Dharina Basri Aramico Basri Aramico, Basri Cintia, Defi Cut Mawar Helmanda Cut Mawar Helmanda Cut Nasrulsyah Cut Novrita Rizki Cut Novrita Rizki Dharina Baharuddin Dilla Santi, Tahara Erlina, Eni Essy Harnelly Fadhillah, Nada Fadhlullah, Fadhlullah Fahmi Ichwansyah Fajriana, Nurul Falita, Cut Mela Fatemah Rosma Fauza, Riska Febyolla Presilawati Hafnidar A. Rani Hafnidar, Hafnidar Hanifah Hasnur Hasnita, Nevi Helmanda, Cut Mawar Hermansyah Hermansyah Hermansyah Hermansyah Hidayat, Melania Ichwansyah, Fachmi Ilham Wahyudi Ira Puspita Sari Irhamna, Dian Ayu Jamaluddin Jamaluddin Kamaliah Kamaliah, Kamaliah Kusma, Nila Lina Rahmawati Loki, Orismi Luthfi Nanda Perdana M. Marthoenis Maghfirah Maghfirah Mahlil Yulian Winda Maidar Maidar Maidar, Maidar Maifrizal, Maifrizal Manja Manja Maria Ulfa Masitoh Masitoh Masrita, Masrita Mauizah Hasanah Mauizah Hasanah Mauizzah Hasanah Mawaddah, Husnul Muhammad Fadhil Muhammad Fikri Muhammad Muhammad Muhammad Yani Muhammad Yani, Muhammad Murdi, Syahrial Muslim Muslim Muslim Muslim MUSLIM MUSLIM Nada Fadhillah Nazariah Nazariah Nazariah Nazariah, Nazariah Nura Shara Amirza Nurhidayatun Rahma Nurjannah Nurjannah Nurul Fajriana Nurul Fajriana Ori Resmida Orismi Loki QURRATU AINI Qurratu, Aini Radhiah Zakaria Radhiah, Radhiah Rahmatun Nisa Rahmatun Nisa, Rahmatun Restu Andrian Restu Andrian, Restu Rina Hasnita Risnawati Risnawati Riza Septiani Riza Septiani Rizki, Cut Novrita Rosnidarwati Rosnidarwati Salsabila, Siti Saputra, Irwan Siti Salsabila Surna Lastri Suryady Suryady Suryady, Suryady Sutri Helfianti Suwarniati Suwarniati, Suwarniati Suwirda, Cut Syarifah Farissi Hamama Syelvita, Cut Yayang Tahara Dilla Santi Ulia Hanum Ulia Hanum Usman, Said Vera Nazhira Arifin Win, Cho Cho Winda, Mahlil Yulian Yosi Rosalita Zaharamutia, Zaharamutia Zairin Thomy Zakaria, Radhiah Zalianda, Rahma