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Journal : Nandur

Isolasi dan Skrining Bakteri Selulolitik pada Feses Luwak I Putu Eko Arymanto Wira Kesuma; I Nyoman Wijaya; Made Sritamin
Nandur Vol 1 No 3 (2021)
Publisher : Fakultas Pertanian, Universitas Udayana

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Abstract

Civet (Paradoxurus hermaphroditus) are carnivorous animals, but also often eat fruit and one of them is coffee cherries. The coffee cherries that are eaten are not completely digested, so the coffee beans come out with the civet feces. In the civet digestion there are various kinds of bacteria that help the digestive process, one of them is cellulolytic bacteria. It is possible that coffee beans in civet feces contain cellulolytic bacteria.This study aims to determine the presence of cellulolytic bacteria in civet feces. The results of bacterial isolation in civet feces obtained 6 isolates that had different morphological characters. Cellulolytic bacteria screening results showed that 2 out of 6 isolates were positive as cellulolytic bacteria, namely FL2 isolate with a cellulolytic index of 2.38 (high reaction) and FL3 isolate with a cellulolytic index of 1.24 (medium reaction). The isolates FL2 and FL3 had different morphological characters in colony shape, margins and color, but had similarities in elevation, gram color and cell shape.
Identifikasi Senyawa Fitokimia pada Daun Kersen (Muntingia calabura Linn.) dan Uji Toksisitasnya Terhadap Larva Udang Artemia salina Leach Ade Febrianto Umbu Romu Kolambani; Made Sritamin; I Nyoman Wijaya
Nandur Vol 1 No 4 (2021)
Publisher : Fakultas Pertanian, Universitas Udayana

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Abstract

Singapore cherry (Muntingia calabura L.) is a plant that has shady trees, is often used as a shade and is easy to grow in various places. All parts of the cherry plant, including roots, bark, fruit, and leaves, contain secondary metabolites that can be used as traditional medicine. The purpose of this study was to determine the phytochemical compounds contained in cherry leaf extract and to determine the level of acute toxicity (LC50) using the Brine Shrimp Lethality Test (BSLT) method. Identification of phytochemical compounds was carried out by gas chromatography-mass spectrometry and acute toxicity test (LC50) was carried out using the BSLT method using the shrimp larvae test insect Artemia salina Leach. The acute toxicity test (LC50) consisted of 6 concentration treatments, namely 500 ppm, 250 ppm, 150 ppm, 100 ppm, 50 ppm, along with negative controls, each of which was repeated three times. The LC50 value is obtained from probit analysis in Microsoft Office Excel. There are 3 groups of bioactive compounds with the highest peak area in singapore cherry leaf extract, namely groups of flavonoids, phenols, and alkaloid. The result of acute toxicity test (LC50) of singapore cherry leaves extract was 84,029 ppm.
Identifikasi Senyawa Fitokimia Kulit Batang Kelor (Moringa oleifera Lamk) Dengan Metode GC-MS dan Uji Toksisitas Terhadap Larva Udang Artemia salina leach Antony Lay; I Gede Putu Wirawan; I Nyoman Wijaya
Nandur Vol 2 No 1 (2022)
Publisher : Fakultas Pertanian, Universitas Udayana

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Abstract

Kelor (Moringa oleifera) is a plant that grows in tropical climates including Indonesia. All parts of this plant can be used as medicine and other natural products. The purpose of this study was to determine the bioactive compounds contained in moringa stem bark and the level of toxicity expressed by an LC50 value of less than 1.000 ppm so that it can be used as traditional medicine and other natural products. Identification of bioactive compounds in moringa stem bark using the GC-MS analysis method and toxicity test on Artemia salina Leach shrimp larvae using the Brine Shrimp Lethality Test (BSLT) method. Based on the results of the analysis using GC-MS showed that moringa bark extract contains 14 bioactive compounds. The highest bioactive compounds in the 3 peaks were secondary metabolites in the form of phenols and monoterpenoids. The toxicity test of moringa bark extract against Artemia salina Leach larvae showed that the higher the concentration, the higher the percentage of mortality of Artemia salina Leach, so that the calculated LC50 value of moringa bark extract was toxic, which was 149,689 ppm.
Isolasi dan Identifikasi Bakteri Selulolitik pada Buah Kopi Arabika (Coffea arabica L.) Nona Au Waling; Made Sritamin; I Nyoman Wijaya
Nandur Vol 1 No 3 (2021)
Publisher : Fakultas Pertanian, Universitas Udayana

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Abstract

Cellulolytic bacteria are microorganisms that can produce cellulase enzymes. Cellulolytic bacteria contain cellulase enzymes that play a role in the cellulose degradation processes. The purpose of this study was to isolate cellulolytic bacteria that capable to degrading cellulose from arabica coffee cherries collected from Catur Village, Kintamani. The results of bacterial isolation at 10-6 and 10-8 dilutions obtained 10 bacterial colonies. From the results of bacterial purification, 4 different bacterial isolates had different morphological characters. The results of the isolation of the colonies showed differences in a round and irregular shape, had raised and convex surfaces had red, cream, white and yellow colors, had curved and smooth edges. Tests were carried out on four bacterial isolates to determine the clear zone index. Only the L isolate in the congo red test had a diameter of clear zone 2.5 mm and with score 3 based on cellulolytic index value L isolate categorized as strong degrading cellulose bacteria.
Kelimpahan Thrips parvispinus Karny dan Musuh Alaminya pada Tanaman Cabai Rawit (Capsicum frutescens L.) I Gede Sumaradana; I Nyoman Wijaya; I Made Mega Adnyana
Nandur Vol 1 No 2 (2021)
Publisher : Fakultas Pertanian, Universitas Udayana

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Abstract

This aims of the research is to search the abudance of T. parvispinus Karny and its natural enemies on Cayenne Pepper (Capsicum frutescens.L). The research started from November 2020 to January 2021 at Melinggih Kelod Village, Payangan District, Gianyar Regency and at the Plant Pest Laboratory, Faculty of Agriculture, Udayana University. The study was conducted using a descriptive method by conducting a diagonal survey of 20 samples of cayenne pepper that were attacked by T. parvispinus. Observations were made by observing 10 leaves and 10 flowers of cayenne pepper plants. The results showed that the abundance of T. parvispinus in the vegetative and generative phases fluctuated where the highest population of T. parvispinus occurred in plants aged 10 week after transplanting with an average population of 15.8 individuals per plant then decreased in plants aged 11 WAT with an average population 9.75 individuals. The results showed that there were two types of predators that preyed on T. parvisvinus, namely Chrysoperla carnea with an average population of 23.3 and Coccinela transversalis with an average of 22.4 individuals. Laboratory reseach showed that the type of parasitoid that emerged from the 100 nymphs that were caught was Ceranisus spp with an average population of 10.2 individuals.
Co-Authors Abdul Rahem Abdul Rahem Abdul Rahem, Abdul Ade Febrianto Umbu Romu Kolambani AGNESIA KURNIAWATI Akhmad Khoirul Munir Aldrik Joe Melvin Tarigan Ana Yuda ANAK AGUNG GEDE GARBA YOGANTARA Andi Hermansyah Andi Hermansyah Antonius Gamma Wardana Antony Lay Aqila Dira Eka Maretha Ar Rayyan, Muhammad Zhafir Argya Arsy Isa Rajendra Arie Sulistyarini Arie Sulistyarini Athayya Indy Nasywa Aulia, Shasky Saffira Awahita, Bahira Aisyah Ayu Anggreini Sitohang Azzahro, Sabrina Aulia Bakar, Nor Kartini Abu Bambang Subakti Z Bambang Tri Purwanto Brandon Budiatin, Aniek Setiya Candrakirana, Sonya Arini Catur Dian Setiawan Cha, Joo Young Chrismawan Ardianto Costa Cruz, Latizio Beni Da Dalfa, Dela Daniel Emmanuel Wijaya Dewi Melani Hariyadi Diana Putri Lestari Dikta, I Putu Gede Asnawa Djoko Agus Purwanto Dwi Martiningsia Dwi Martiningsia DWI WIDANINGSIH Einarel Yusufrizky Wibisono Ekawati, Ni Enjelina, Ni Desak Gede Rista Fakhriatun Nisa Fany Zumrotul Faizah Farah Mahdiyyah Farhan Hanang Ichsanto Farida Ifadotunnikmah Fatmawati, Anjar Felisya, Anggi Ratu Gayatri, Anak Agung Sagung Intan GEDE SUASTIKA GEDE WIJANA Gesnita Nugraheni Giantari, I Gusti Ngurah Alit Susanta Wirya Gusti Noorrizka V Gusti Noorrizka Veronika Ahmad Hadi Poerwono Hamdri, Apta Amalia Handayani, Rosita Hanni Prihhastuti Puspitasari Hayuningtyas, Eka Putri Hermansyah, Andi Husni dan Zulfanazli Pudjianto Alfian Rusdy I Dewa Nyoman Nyana I Gde Antha Kasmawan I GEDE BAGUS DWIPANANDA I GEDE PUTU WIRAWAN I Gede Sumaradana I Gusti Ayu Diah Yuniti I Gusti Ngurah Bagus I Ketut Siadi I Ketut Suada I KETUT SUMIARTHA I Komang Budaarsa I Komang Widya Purnama Yasa I Made Gede Anadhi I Made Mega Adnyana I MADE SUDARMA I MADE SUDARMA I Nyoman Darma Putra I NYOMAN RAI I Nyoman Suarka I Nyoman sukiada I NYOMAN SUKIADA I Nyoman Wijaya I Putu Agus Hendra Wibawa I Putu Anom I Putu Eko Arymanto Wira Kesuma I Putu Sudiarta I Wayan Ardika I WAYAN SASTONO I Wayan Suardiana I Wayan Supartha Ida Ayu Dwi Giriantari Iman, Evelin Nicole Juni Ekowati Kamila, Azzihra Rahma KETUT AYU YULIADHI Ketut Srie Marhaeni Julyasih Lesmana, Anindya Nadine Liza Pristianty LUH TINA ARYANTINI M. Rahmad M. Rahmad, M. Rahmad MADE SRITAMIN Madona Dwi Fitriani MAGNA DWIPAYANA MAHARDHIKA DWI PUTRA WIJAYA Mahardian Rahmadi Mancika, Ni Made Juliana Dewi Marcellino Rudyanto Mardani, Widya Tama Melva Santi Sihaloho Mintari, Gusti Ayu Willem MISBAHUL KHULUQ MOCH BINTANG RAMADHAN Mohammad Hefni Hidayat Mufarrihah, Mufarrihah Muharrom Riezky Prasetyo Munthe, Lambok Jenny Sagita Nababan, Evan Gabriel Nathganeila, Luthfia Andini Naufal Athaullah Aulia Naufal Dhifari Ramadhan Nawal Karina Basalamah Nazwa Zahira Anwar NEVIATY PUTRI ZAMANI NI KETUT JULI DWI JAYANTI Ni Made Puspawati Ni Made Regina Prasetya Putri NI MADE TRIGUNASIH NI NENGAH DARMIATI NI NYOMAN ARI MAYADEWI NI PUTU SWARI MEITAYANI NI WAYAN ARMINI NI WAYAN PUSPARINI DHARMAPUTRI NI WAYAN SUNITI Ningsih, Gusti Ketut Asti Dea Nona Au Waling Noorma Rosita Noorrizka V, Gusti Norine Nitya Ardiningrum Novayolla, Alsha Nurftatmelia Nurfadzilah, Puteri Gisyela NURUL KHOTIMAH NYOMAN AYU TRISNA ARI UTAMI OCTA FRANSISCA SITORUS Oktaviani, Faradila Putri Parwata, I Made Oka Pranata, Kadek Dwipayana Priyandani, Yuni Purwitasari, Neny PUTU PADMA MAHESWARI Qanita Afrannajla Rafid Khairi Santoso Rahayu, Titik Puji Rahmani, Risma Sekar RAMADHAN, MOCH BINTANG RIDA MELANI Rika Uyunul Mabilla RISMA IMROATUS SHOLIHAH Sabila, Bilqis Rif'atus Safitri, Zelda Santoso, Aina Cahyaning Sasadara, Maria Malida Vernandes Siahaan, Enjelina Silfa Fajriyatur Rosyidah Siti Rahmah Sri Lestari SRI LESTARI Sugiarto, Jennifer Angeline Sugijanto Sugijanto Sukaatmadja, I Suko Hardjono Sukorini, Anila Impian Sumarno . Suzana Syalsabila Aisyah Rahmawati Tarsat, Mosli Tho'ati, Fitri Nur Hidayatuth Timothy Alessandro Christaluary Lanapu Titania Fiska Ornelia Toetik Aryani Tri Widiandani TRISNA AGUNG PHABIOLA TRISNA AGUNG PHABIOLA Tristiana Erawati Tuan, Nguyen Thanh Tutiek Purwanti Umi Athiyah Umi Athiyah, Umi Ursulla Yulananda Anugraheni UTAMA, I WAYAN EKA KARYA Vanessa Emas Permata Sari VANI SILVANA Vaturrachma, Amelia Safira Putri VLORA VERONICA SIPANGKAR Wahyu Utami Wahyu Utami Wahyu Utami, Wahyu Walidah, Ziyana Wardah Zuhan Nafikhah WAYAN ADIARTAYASA WIDHIANTHINI WIDHIANTHINI Widiarsana Putra, I Made Widji Soeratri Wigunanda, I Wayan Surya Aditya Wiraatmaja, Wayan Wiwied Ekasari Yogi, Ida Bagus Putu Prajna YUDHA, I KADEK WISMA Yuni Priyandani Yuni Priyandani Yunita Nita Yusuf, Helmy Zhao, Tiejun Zulkamain, Bambang Subakti Zulkarnain, Bambang Subakti