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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Akuakultur Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Jurnal Veteriner ILMU KELAUTAN: Indonesian Journal of Marine Sciences Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Torani Journal of Fisheries and Marine Science Jurnal Natur Indonesia Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Omni-Akuatika Proceeding Simposium Nasional Kelautan dan Perikanan Aquasains : Jurnal Ilmu Perikanan dan Sumberdaya Perairan Indonesian Aquaculture Journal Jurnal Riset Akuakultur Jurnal Kelautan Nasional Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Indonesian Journal of Electrical Engineering and Computer Science Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan

M. Zairin Junior

Departemen Budidaya Perairan, Fakultas Perikanan Dan Ilmu Kelautan, Institut Pertanian Bogor

Author-ID : 293865

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Social Sciences Veterinary Other

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The population growth and the nutritional status of Moina macrocopa feed with rice bran and cassava bran suspensions Mubarak, Ahmad Shofy; Jusadi, Dedi; Junior, Muhammad Zairin; Suprayudi, Muhammad Agus
Jurnal Akuakultur Indonesia Vol. 16 No. 2 (2017): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Moina macrocopa culture density can be improved by optimizing the fecundity, and somatic growth through the regulation of quality and quantity of feed. The purpose of this study were to determined how to use effectively the rice bran and cassava bran Manihot utilisima suspension on Moina, based on population, neonates production, adult percentage, biomass, metabolisme and nutritional state. In this study, Moina were cultured for eighth days using four concentrations of rice bran suspension and three concentrations of cassava suspension. This research found that M. macrocopa culture with rice bran suspension has higher population, neonates production, adult percentage and biomass than its culture with cassava bran suspension (P<0.05). This study also found that Moina culture with rice bran suspension has higher total value of RNA, total value of DNA, the ratio RNA/ DNA, FCR, and concentration of protein and amino acid than Moina culture with cassava bran suspension. Treatment D with the initial rice bran suspension concentration was 0.3 mL/L and was increased starting the second day and the end concentration on the eighth day was 1.2 mL/L has highest peak population of Moina 17,975 ind/L in seventh day, weight wet biomass 439 mg/L in eighth day and lower FCR 0.94. Keywords: suspension, rice bran, cassava, population, ratio RNA/DNA ABSTRAK Kepadatan populasi dalam budidaya Moina macrocopa dapat ditingkatkan dengan mengoptimalkan fekunditas dan pertumbuhan somatik melalui pengaturan kualitas dan kuantitas pakan. Penelitian ini bertujuan untuk menganalisis efektifitas pengunaan pakan suspensi dedak dan tepung ketela pohon Manihot utilisima dalam budidaya M. macrocopa terhadap populasi, produksi anak per induk, persentase dewasa, biomasa, FCR, dan metabolismenya (asam amino, DNA, RNA, dan RNA/DNA). Di dalam penelitian ini, M. macrocopa dibudidayakan selama delapan hari menggunakan empat konsentrasi suspensi dedak dan tiga konsentrasi suspensi tepung ketela pohon. Hasil penelitian ini menunjukkan bahwa, budidaya M. macrocopa dengan pakan suspensi dedak menghasilkan populasi, produksi anak/induk, persentase dewasa dan biomasa yang lebih tinggi dibandingkan budidaya Moina dengan pakan suspensi ketela pohon (P<0,05). Budidaya M. macrocopa dengan pakan suspensi dedak juga menghasilkan total RNA, total DNA dan nisbah RNA/DNA, konsentrasi protein, dan asam amino yang lebih tinggi dibandingkan Moina dengan pakan suspensi ketela pohon. Perlakuan D dengan pakan suspensi dedak awal 0,2 mL/L dan meningkat mulai hari kedua dengan konsentrasi hari kedelapan 1,2 mL/L menghasilkan puncak populasi tertinggi pada hari ketujuh sebanyak 17.975 ind/L, berat basah biomasa hari kedelapan kultur 439 mg/L, dan FCR yang rendah yaitu 0,94. Kata kunci: suspensi, dedak, ketela pohon, populasi, nisbah RNA/DNA

Stress responses of transportation on red tilapia which given feed containing chromium Rakhmawati, Rakhmawati,; Suprayudi, Muhammad Agus; Setiawati, Mia; Widanarni, Widanarni,; Junior, Muhammad Zairin; Jusadi, Dedi
Jurnal Akuakultur Indonesia Vol. 17 No. 1 (2018): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTThis study was conducted to evaluate stress responses of transportation on red tilapia Oreochromis sp. which given feed containing chromium. Three isonitrogenous and isocaloric experimental feeds were prepared, these diets were control (without chromium), CrPic 1 mg/kg, and CrYst 2 mg/kg supplementation in feed, all group were arranged triplicate. Satiation feeding was done three times a day. After a 60-day feeding experiment, the experimental fishes were fasted and distributed in polyethylene bags (N=60 fish/bag) containing 3 L of water, subjected to condition of transport simulation for 13 hours. Survival rate, levels of plasma cortisol, blood glucose, superoxide dismutase (SOD), and malondialdehyde (MDA) enzyme were observed at before transportation, after transportation, one day, and two days after transportation. The result showed that chromium supplementation reduced the levels of plasma cortisol before and after transportation, one day, and two days after transportation. Also, it decreased blood glucose compared with control significantly before transportation and one day after transportation. The SOD enzyme concentration increased significantly after fish was fed with feed containing chromium for 30 days, while the MDA enzyme concentration increased significantly after two days of transportation. However, there was no significant difference in the survival of red tilapia between treatments. The best result was obtained in the treatment of fish which fed with feed containing chromium. CrPic supplementation 1 mg/kg and CrYst 2 mg/kg increased the body resistance in red tilapia by decreasing the negative effect of stress while transportation. Keywords: stress, transportation, red tilapia, chromium ABSTRAKPenelitian dilakukan untuk mengevaluasi respons stres transportasi ikan nila merah Oreochromis sp. yang diberikan pakan yang mengandung kromium. Pada penelitian ini digunakan tiga jenis pakan, terdiri atas pakan tanpa suplementasi kromium (kontrol), pakan bersuplementasi kromium pikolinat (CrPic 1 mg/kg), dan kromium yeast (CrYst 2 mg/kg), semua perlakuan diulang sebanyak tiga ulangan. Pemberian pakan sebanyak tiga kali sehari dan dilakukan secara at satiation. Setelah 30 hari pemeliharaan, ikan uji dipuasakan dan didistribusikan dalam plastik polietilen (N=60 ekor ikan/kantong plastik) yang berisi 3 L air, dilakukan dengan simulasi transportasi selama 13 jam. Parameter yang diamati pada penelitian ini adalah kelangsungan hidup, kortisol, glukosa darah, enzim superoksida dismustase (SOD), dan malondialdehida (MDA) saat sebelum transportasi, sesaat setelah transportasi, sehari, dan dua hari setelah transportasi. Hasil yang didapatkan adalah suplementasi kromium menurunkan konsentrasi kortisol secara signifikan sebelum transportasi, sesaat, sehari, dan dua hari setelah transportasi. Suplementasi kromium menurunkan glukosa darah secara signifikan pada saat sebelum transportasi dan sehari setelah transportasi. Konsentrasi enzim SOD meningkat secara signifikan setelah pemberian pakan bersuplementasi kromium selama 30 hari, sedangkan konsentrasi enzim MDA meningkat secara signifikan setelah dua hari transportasi pada ikan yang diberi pakan bersuplementasi kromium. Namun, tidak ada perbedaan yang signifikan pada kelangsungan hidup ikan nila merah antarperlakuan. Hasil terbaik diperoleh pada perlakuan ikan dengan suplementasi kromium. Suplementasi 1 mg/kg CrPic dan 2 mg/kg CrYst dapat meningkatkan daya tahan tubuh pada budidaya ikan nila merah dengan menurunkan pengaruh negatif stres akibat transportasi. Kata kunci: stres, transportasi, nila merah, kromium

Biochemical study of striped catfish Pangasianodon hypophthalmus broodstock induced by PMSG hormone + anti‒dopamine and turmeric addition Arfah, Harton; Sudrajat, Agus Oman; Suprayudi, Muhammad Agus; Junior, Muhammad Zairin
Jurnal Akuakultur Indonesia Vol. 17 No. 2 (2018): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT This study aimed to evaluate biochemical changes (cholesterol, triglyceride, HDL, LDL, glucose, and plasma protein) on striped catfish Pangasianodon hypophthalmus broodstock induced with PMSG hormone and turmeric addition. An observation was also done to blood glycogen content. The striped catfish broodstock was fed on commercial feed without any addition (control) and with turmeric addition (HKu). In control treatment, there was a decreasing on cholesterol, meanwhile, the triglyceride (TG) value was increased. The HDL concentration was decreased in 2nd sampling and increased in 4th sampling. In 1st until 4th sampling, glucose was quite stable, while LDL was on extremely low concentration. In HKu treatment, the cholesterol value was higher than the control treatment. The TG concentration also higher than control in 3rd sampling and decreased in 4th sampling. The HDL concentration was increased and higher than the control treatment, while LDL concentration was lower. The liver glycogen content on the control and HKu treatment were 0.015 (mg/100 mL) and 0.181 (mg/100 mL) respectively; while in the flesh of the control and HKu treatment were 0.76 (mg/100 mL) and 1.19 (mg/100 mL) respectively; and in the gonad of control and HKu treatment were 0.10 (mg/100 mL) and 0.70 (mg/100 mL) respectively. It was shown that the glycogen content in the liver, flesh, and gonad on experimental fish was higher than control treatment. Keywords : biochemistry, hormone, turmeric, channel catfish, reproduction ABSTRAK Penelitian ini bertujuan untuk mengkaji perubahan biokimia (kolesterol, trigliserida, HDL, LDL, glukosa dan protein plasma) induk ikan patin Pangasianodon hypophthalmus yang diberi perlakuan hormon PMSG dan kunyit (HKu). Pengamatan juga dilakukan terhadap glikogen dalam darah induk patin. Induk ikan patin diberi pakan tanpa penambahan kunyit (kontrol) dan pakan yang diberi HKu. Hasil penelitian pada kontrol menunjukkan adanya penurunan kolesterol, sedangkan pada TG mengalami peningkatan. HDL menurun pada sampling ke‒2 dan meningkat pada sampling ke‒4. Sementara itu, pada LDL rendah sekali dan pada glukosa terlihat stabil dari sampling ke‒1 sampai ke‒4. Pada perlakuan HKu terlihat bahwa pada kolesterol menghasilkan nilai lebih tinggi dibanding kontrol. Pada TG terlihat juga nilai lebih tinggi dibanding kontrol pada sampling ke‒3 dan menurun pada sampling ke‒4. Konsentrasi HDL meningkat dan lebih tinggi dibanding kontrol, sedangkan nilai LDL lebih rendah. Data yang diperoleh pada kadar glikogen hati perlakuan kontrol adalah 0,015 (mg/100 mL) dan HKu 0,181 (mg/100 mL); sedangkan pada daging kontrol sebesar 0,76 (mg/100 mL) dan HKu 1,19 (mg/100 mL); serta gonad kontrol 0,10 (mg/100 mL) dan HKu 0,70 (mg/100 mL). Hal ini menunjukkan kadar glikogen pada hati, daging, dan gonad ikan yang diberi perlakuan bernilai lebih tinggi dibanding kontrol. Kata kunci : biokimia, hormon, kunyit, ikan patin, reproduksi

Stimulation of gonad maturation in mullet fish Mugil dussumieri using MT, E2, hCG, and Ovaprim hormone Cahyono, Tatak Dwi; Junior, Muhammad Zairin; Carman, Odang
Jurnal Akuakultur Indonesia Vol. 18 No. 1 (2019): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The present study is a preliminary research for producing mullet fry to aquaculture. The research aimed to evaluate stimulation of gonad maturation in mullet (Mugil dussumieri) using hormones. The method used for research was completely randomized design consisting of three treatments and each individual replication was repeated three times. Two experiments were conducted separately with different treatments. First experiment used 9-14.7 cm body length of fish treated with different hormone injections i.e. 4 mg/kg 17α-methyltestosterone (MT), 0.07 mg/kg estradiol-17β (E2), and 0.5 ml/kg 0.9% physiological solution as control. Second experiment used 10-31 cm body length fish treated with 750 IU/kg chorionic gonadotropin (hCG), 0.5 ml/kg Ovaprim, and 0.9% physiological solution as control. Gonadosomatic index (GSI) value of the first experiment on day 60 showed that GSI of E2 treatment (1.31±0.94%) was higher than both MT treatment (1.00±0.51%) and control (0.54±0.20%). On the other hand, the second experiment on day 60 showed that GSI of hCG treatment (7.18±0.59%) was higher than both Ovaprim treatment (3.29±2.66%) and control (6.72±0.32%). Egg diameter frequency distribution for control in the first experiment on day 30 showed that egg size ranged from 9−144 µm. Egg diameter for E2 and MT treatments on day 60 showed that egg size ranged from 9−243 µm and were higher than control. In the second experiment, egg diameter on day 30 for control showed that egg size ranged from 9-144 µm, hCG treatment showed egg size ranged from 9−441 µm, while Ovaprim egg size ranged from 9-111 µm. Blood glucose, blood cholesterol, testosterone and estradiol hormone level in the first and second experiment showed no significant difference. The results showed that estradiol-17β and 17α-methyltestosterone induction in 9-14.7 cm body length mullet increase gonad maturity to stage II while hCG induction in 10-31 cm body length mullet increase gonad maturity to stage III. Keywords : estradiol-17β, hCG, 17α-methyltestosterone, Mugil dussumieri, Ovaprim ABSTRAK Penelitian merupakan rintisan untuk menghasilkan benih ikan belanak dalam wadah budidaya. Penelitian ini bertujuan untuk mengevaluasi pematangan gonad ikan belanak Mugil dussumieri menggunakan hormon. Penelitian dilakukan menggunakan rancangan acak lengkap yang terdiri dari tiga perlakuan dan tiga kali ulangan individu. Terdapat dua percobaan dengan perlakuan berbeda dan dilakukan secara terpisah. Percobaan pertama menggunakan ikan berukuran 9−14.7 cm dengan hormon 17α-metiltestosteron (MT) 4 mg/kg, estradiol-17β (E2) 0.07 mg/kg dan kontrol larutan fisiologis 0.9% 0.5 ml/kg. Percobaan kedua menggunakan ikan berukuran 10−31 cm dengan human chorionic gonadotropin (hCG) 750 IU/kg, Ovaprim 0.5 ml/kg dan kontrol larutan fisiologis 0.9 % 0.5 ml/kg. Nilai gonadosomatic index (GSI) percobaan pertama pada hari ke-60 menunjukkan bahwa pemberian estradiol-17β (1.31±0.94%) lebih tinggi dibandingkan 17α-metiltestosteron (1.00±0.51%) dan kontrol (0.54±0.20%). Hasil percobaan kedua pada hari ke-60 nilai GSI menunjukkan bahwa pemberian hCG (7.18±0.59%) lebih tinggi dibandingkan Ovaprim (3.29±2.66%) dan kontrol (6.72±0.32%). Sebaran frekuensi diameter telur pada percobaan pertama untuk kontrol hari ke-30 menunjukkan kisaran 9−144 µm. Sebaran frekuensi diameter telur untuk estradiol-17β dan 17α-metiltestosteron hari ke-60 menunjukkan kisaran 9−243 µm lebih banyak dibandingkan kontrol. Sebaran frekuensi diameter telur pada percobaan kedua untuk kontrol hari ke-30 menunjukkan kisaran 9−144 µm, hCG 9−441 µm sedangkan Ovaprim hanya 9−111 µm. Kadar glukosa darah, kolesterol darah, hormon testosteron dan estradiol pada percobaan pertama dan percobaan kedua menunjukkan tidak terdapat perbedaan yang nyata. Hasil penelitian menunjukkan bahwa induksi hormon estradiol-17β dan 17α-metiltestosteron pada ikan berukuran 9−14.7 cm dapat meningkatkan kematangan gonad mencapai TKG II sedangkan induksi hormon hCG pada ikan berukuran 10−31 cm dapat meningkatkan kematangan gonad mencapai TKG III. Kata kunci : estradiol-17β, hCG, 17α-metiltestosteron, Mugil dussumieri, Ovaprim

Enhancement of colour quality, growth, and health status of rainbow Kurumoi fish Melanotaenia parva through dietary synthetic carotenoids supplementation Meilisza, Nina; Suprayudi, Muhammad Agus; Jusadi, Dedi; Junior, Muhammad Zairin; Artika, I Made; Utomo, Nur Bambang Priyo
Jurnal Akuakultur Indonesia Vol. 18 No. 1 (2019): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Carotenoids were known as pigment sources, the precursor of vitamin A, potential antioxidant and can improve the health status of fish. Furthermore, there are also studies that reveal the role of carotenoids in bone formation and metabolism. This study was conducted to determine the effect of different carotenoids at two different doses in the feed on growth, pigmentation, vitamin A conversion, blood profile, antioxidant activity, and calcium bone of the rainbow fish. Twenty-one aquariums with a volume of 20 L each stocked with 20 fish (1.08 ± 0.03 g of body weight and 4.56 ± 0.07 cm of body length). The experiment applied seven experimental diets (in triplicates) consisted of three types of carotenoids were astaxanthin (AS), canthaxanthin (CS), and lutein (LS) and two doses (130 and 260 mg/kg), i.e. AS-130, AS-260, CS-130, CS-260, LS-130, LS-260 and basal (without carotenoids) as the control. The fish were fed for 56 days of experimental period. The results showed that carotenoid diets were able to increase growth, total carotenoids, percentages of chromatophores, vitamin A conversion, erythrocyte, leukocytes, packed cell volume (PCV), neutrophils, and hemoglobin (Hb) compared to the control. Fish fed dietary astaxanthin at a level of 260 mg/kg was superior compared to other diets. Dietary carotenoids were also capable of decreasing the endogenous antioxidant activity of superoxide dismutase (SOD) and malonyl dialdehyde (MDA) and increased the calcium level in fish bone than basal diet. Keywords: carotenoids, growth, health status, Melanotaenia parva, pigmentation ABSTRAK Karotenoid diketahui sebagai sumber pigmen, prekursor vitamin A, antioksidan potensial dan dapat meningkatkan status kesehatan ikan. Selain itu, karotenoid juga memiliki peran dalam formasi dan metabolism tulang. Penelitian ini dilakukan untuk mengevaluasi pengaruh jenis dan dosis karotenoid yang berbeda terhadap pertumbuhan, pigmentasi, konversi vitamin A, gambaran darah, aktifitas antioksidan dan kalsium tulang ikan rainbow Kurumoi. Sebanyak 20 ekor ikan (bobot tubuh rata-rata 1,08 ± 0,03 g dan panjang total rata-rata 4,56 ± 0,07 cm) dan diberi makan pakan yang mengandung karotenoid. Pakan uji terdiri atas tiga jenis karotenoid dengan tiga ulangan yaitu astaksantin (AS), cantaksantin (CS), dan lutein (LS) dan dua dosis (130 dan 260 mg/kg) dikodekan dengan AS-130, AS-260, CS-130, CS-260, LS-130, LS-260 dan basal (tanpa karotenoid) sebagai kontrol. Ikan diberi makan selama 56 hari pemeliharaan. Hasil penelitian menunjukkan bahwa karotenoid dapat meningkatkan pertumbuhan, total karotenoid, persentase kromatofora, konversi vitamin A, eritrosit, leukosit, hematokrit, neutrofil, dan hemoglobin dibandingkan dengan kontrol. Ikan yang diberi pakan mengandung astaksantin 200 mg/kg lebih baik dibandingkan denga pakan uji lainnya. Pakan yang mengandung karotenoid juga mampu menurunkan antioksidan endogenus superoxide dismutase (SOD) dan malonyl dialdehyde (MDA), serta meningkatkan kalsium tulang ikan dibandingkan pakan kontrol. Keywords: Melanotaenia parva, karotenoid, pertumbuhan, pigmentasi, status kesehatan.

Identification and expression analysis of c-type and g-type lysozymes genes after Aeromonas hydrophila infection in African catfish Nasrullah, Hasan; Nababan, Yanti Inneke; Yanti, Dwi Hany; Hardiantho, Dian; Nuryati, Sri; Junior, Muhammad Zairin; Ekasari, Julie; Alimuddin, Alimuddin
Jurnal Akuakultur Indonesia Vol. 18 No. 2 (2019): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTLysozymes play an important role in the first line of defense in fish and potentially used as an immunity status biomarker and immune responses evaluation in fish, which often found in two types, i.e. chicken-type and goose-type (c- and g-type, respectively). To recent, the information related to the sequences and the expression analysis of the c- and g-type lysozyme genes in African catfish is still limited. In the present study, we report a partial cloning and mRNA expression analysis of c-type and g-type lysozymes in African catfish Clarias gariepinus. We have successfully cloned and partially identify the c-type, and g-type lysozyme genes of C. gariepinus, which consist of 594 and 560 of coding sequences, respectively. Catalytic and other conserved residues were identified by multiple sequences alignment and they showed high similarity with other teleost fish species. mRNA levels of the genes were analyzed by using qPCR method and their expressions in the spleen, liver, and head kidney were rapidly modulated after Aeromonas hydrophila injection, with different patterns were observed in each organ. These results confirmed that c- and g-type lysozymes played an important role in non-specific immunity against A. hydrophila infection. This study provided valuable information that can be used to understand the African catfish immune systems for better disease and stress management in C. gariepinus culture.Keywords: lysozymes, gene identification, gene expression, bacterial infection, African catfish ABSTRAKLisozim berperan dalam sistem pertahanan dini pada ikan dan sangat potensial digunakan sebagai marka status imunitas dalam evaluasi respons imun. Lisozim umum ditemukan dalam dua tipe pada ikan: tipe-ayam (tipe-c) dan tipe-angsa (tipe-g). Informasi terkait sekuens gen dan ekspresi gen kedua tipe lisozim pada ikan lele dumbo sangat terbatas. Pada penelitian ini, kami melaporkan kloning gen secara parsial, dan analisis ekspresi gen dari kedua tipe lisozim pada ikan lele dumbo C. gariepinus. Sekuens parsial gen lisozim tipe-c dan tipe-g yang berhasil diidentifikasi adalah sepanjang 594 dan 560 pasang basa. Situs katalitik dan residu khas memiliki tingkat kesamaan yang tinggi dengan spesies ikan yang lain. Analisis mRNA dilakukan dengan metode quantitative PCR (qPCR). Ekspresi kedua gen di jaringan ginjal depan, limpa, dan hati dengan cepat terinduksi pasca infeksi bakteri A. hydrophila dengan pola yang berbeda. Hasil ini menunjukkan bahwa lisozim tipe-c dan tipe-g memiliki peran yang sangat penting dalam sistem imun ikan lele dumbo terhadap infeksi A. hydrophila. Penelitian ini menghasilkan informasi penting yang dapat digunakan untuk mempelajari sistem imun ikan lele dumbo dan sebagai acuan dalam penanganan dan manajemen penyakit pada budidaya ikan lele dumbo.Kata kunci: lisozim, identifikasi gen, ekspresi gen, infeksi bakteri, ikan lele dumbo

Profile of 17ß-estradiol, vitellogenin, and egg diameter during gonad maturation process of striped catfish Pangasianodon hypophthalmus Pamungkas, Wahyu; Jusadi, Dedi; Junior, Muhammad Zairin; Setiawati, Mia; Supriyono, Eddy; Imron, Imron
Jurnal Akuakultur Indonesia Vol. 18 No. 2 (2019): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT This study was conducted to evaluate the profile of 17ß-estradiol (E2) and vitellogenin (Vtg) in plasma and egg diameter during gonad maturity process of striped catfish Pangasianodon hypophthalmus. Blood samples were collected from immature striped catfish, male and female with different stage of gonad maturity (stage I, II, III, and IV) to measure the concentrations of E2 and Vtg. Gonad maturity development of striped catfish was observed based on egg diameter. Result showed that E2 concentrations were the highest (843.65 pg/mL) on female with maturity stage III, the lowest on the male (26.34 pg/mL), and immature female fish (29.37 pg/mL). The protein band of Vtg was obtained on the plasma of the mature female (stage I, II, III and IV) with a molecular weight (MW) between 140−180 kDa, but it was not obtained on immature female dan male striped catfish. The highest concentration of Vtg was found in the plasma of the female fish with maturity stage III (87.34 mg/mL), then on the stage II (74.83 mg/mL), I (68.58 mg/mL), and IV (33.45 mg/mL). It showed that egg yolk formation occurred in the female mature. The average egg diameter was 0.107 ± 0.052 mm, 0.318 ± 0.086 mm, 0.864 ± 0.099 mm, and 1.041 ± 0.058 mm on the maturity stage I, II, III, and IV respectively. The increase of egg diameter along with development of gonad maturity stage indicated that egg development occurred due to the process of vitellogenesis and the addition of egg yolk on oocyte. Keywords : egg diameter, gonad maturity, striped catfish , 17ß–estradiol, vitellogenin ABSTRAK Penelitian dilakukan untuk mengevaluasi profil estradiol-17β (E2), vitelogenin (Vtg) dalam plasma dan diameter telur pada proses pematangan gonad ikan patin siam (Pangasianodon hypophthalmus). Sampel darah untuk pengukuran konsentrasi E2 dan Vtg plasma diperoleh dari ikan patin siam betina yang belum matang gonad, ikan jantan, ikan betina dengan tahap kematangan gonad yang berbeda (tahap I, II, III dan IV). Perkembangan kematangan gonad ikan patin siam diamati berdasarkan diameter telur. Hasil penelitian menunjukkan bahwa konsentrasi E2 tertinggi (843,65 pg/mL) pada ikan betina dengan kematangan tahap III, terendah pada ikan jantan (26,34 pg/mL), dan ikan betina tidak matang gonad (29,37 pg/mL). Pita protein Vtg pada sampel plasma diperoleh dari betina matang gonad (tahap I, II, III dan IV) dengan berat molekul antara 140-180 kDa, tetapi tidak diperoleh pada ikan patin siam betina yang belum dewasa dan jantan. Nilai konsentrasi tertinggi Vtg ditemukan dalam plasma darah ikan betina dengan tingkat kematangan III (87,34 mg/mL) kemudian pada tahap II (74,83 mg/mL), I (68,58 mg/mL) dan IV (33,45 mg/mL). Hal ini menunjukkan bahwa pada ikan betina dewasa terjadi proses pembentukan kuning telur (vitelogenesis). Rata-rata diameter telur adalah 0,107 ± 0,052 mm, 0,318 ± 0,086 mm, 0,864 ± 0,099 mm dan 1,041 ± 0,058 mm pada tingkat kematangan I, II, III dan IV secara berurutan. Peningkatan nilai diameter telur seiring dengan perkembangan tahap kematangan gonad menunjukkan bahwa perkembangan telur terjadi karena proses vitelogenesis dan penambahan bahan kuning telur pada oosit. Kata kunci : diameter telur, 17ß-estradiol, kematangan gonad, patin siam, vitelogenin

Aggressive and cannibalistic behavior of African catfish larvae: effect of different doses of methyltestosteron injecting to female broodstock and larval stocking densities Rahmadiah, Triayu; Junior, Muhammad Zairin; Alimuddin, Alimuddin; Diatin, Iis
Jurnal Akuakultur Indonesia Vol. 18 No. 2 (2019): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT This study aimed to evaluate the effect of 17α-methyltestosterone hormone (MT) injecting to female broodstocks and stocking densities on the aggressive and cannibalistic behavior of African catfish larvae. Two-day-old post-hatching larvae were used in this experiment. Larval rearing was started at three-day-old post-hatching (body weight 0.004 ± 0.003 g and total length 0.2 ± 0.05 cm) in a 40 cm×30 cm× 25 cm rearing aquaria. This study used a completed randomized factorial design which consisted of two factors. The first factor were the larvae from females broodstocks without MT injection (0 μg/g body weight) (A), injected with MT (1 μg/g body weight) (B), and injected with MT (2 μg/g body weight) (C), while the second factor were larval stocking densities of 3.000/m2 (V1) and 6.000/m2 (V2), with three replications. The results showed that the larvae from female broodstocks injected with MT 2 μg/g body weight (C) and stocking densities 6.000/m2 (V2) increased the aggressiveness of swim and decreased cannibalism. The highest cannibalistic behavior occurred from 00.00‒06.00.Keyword: aggressiveness, cannibalism, Clarias gariepinus, methyltestosterone ABSTRAKPenelitian ini bertujuan untuk mengevaluasi pengaruh hormon 17α-metiltestosteron (MT) yang diinjeksikan pada induk betina dan padat tebar terhadap tingkat agresivitas dan kanibalisme larva ikan lele. Ikan uji yang digunakan adalah larva berumur dua hari setelah penetasan. Pemeliharaan larva dimulai saat larva berumur tiga hari setelah penetasan (bobot tubuh 0,004 ± 0,003 g dan panjang total 0.2 ± 0.05 cm) yang dipelihara di dalam akuarium berukuran 40 cm×30 cm×25 cm. Penelitian ini menggunakan rancangan acak lengkap faktorial yang terdiri atas dua faktor. Faktor yang pertama adalah larva dari induk tanpa diinjeksi dengan hormon MT (0 μg/g bobot tubuh) (A), diinjeksi dengan hormon MT (1 μg/g bobot tubuh) (B), dan diinjeksi dengan hormon MT (2 μg/g bobot tubuh) (C), sedangkan faktor yang kedua adalah padat tebar 3.000 ekor/m² (V1) dan 6.000 ekor/m² (V2), dengan tiga kali ulangan. Hasil penelitian menunjukkan bahwa larva ikan lele dari induk yang diinjeksi hormon MT dosis 2 µg/g bobot tubuh dan dipelihara pada padat tebar tinggi 6.000 ekor/m² (V2) dapat meningkatkan agresivitas berenang dan menurunkan kanibalisme. Tingkat kanibalisme tertinggi terjadi pada pukul 24.00‒06.00 WIB. Kata kunci: agresivitas, kanibalisme, Clarias gariepinus, metiltestosteron

Portrait of Hatchery Management Profile on Rainbow Fish Melanotaenia boesemani (Allen & Cross, 1980) Cultivation in Jakarta Area Nugraha, Media Fitri Isma; Avarre, Jean-Christophe; Pouyaud, Laurent; Kadarusman, Kadarusman; Carman, Odang; Junior, M. Zairin
Torani Journal of Fisheries and Marine Science VOLUME 1 NOMOR 2, JUNI 2018
Publisher : Hasanuddin University

Hatchery managers and maintaining genetic diversity and fitness population in endangered and threatened species in pond is a important and difficult thing to do. But is must to do for conservation biology. Fitness and structure population depends on effective breeding number (Ne) and population connectivity between each other. The second most important thing is gene flow and genetic drift. Ne is major role in the maintenance of genetic diversity as indicator for inbreeding depresion and genetic drift. We sampled 6 hatchery in Jakarta and Bekasi Indonesia region and used 12 polymorphic microsatellite loci to quantify population genetic structure. Result in this study are, each farm have different methode. The old farmers is bapak Gusi and bapak Hasan (+30 years). Ne in bapak Hasan and bapak Gusi hatchery is 66,667 and F value is 0,00749 and loosing allele (P) is 0,26183. Compared with younger farmers bapak Yahya (5 years) have Ne = 133,333, F = 0,00375 and P = 0,06855. Even though hatchery bapak Hasan and bapak Gusi have the same value Ne, F and P but the result in population structure they are different founder populations. Hatchery bapak Hasan have unique structure and alleles composition compared with other hatchery.Keywords: breeding, management, Melanotaenia boesemani, hatchery, population.

An efficient DVHOP localization algorithm based on simulated annealing for wireless sensor network Arroub, Omar; Darif, Anouar; Saadane, Rachid; Rahmani, My Driss; Aarab, Zineb
Indonesian Journal of Electrical Engineering and Computer Science Vol 39, No 1: July 2025
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijeecs.v39.i1.pp720-736

In the last decade, the research community has devoted significant attention to wireless sensor networks (WSNs) because they contribute positively to some critical issues encountered in nature and even in industry. On the other hand, localization is one of the most important parts of WSN. Hence, the conception of an efficient method of localization has become a hot research topic. Lastly, it has been invented, a set of optimal positioning methods that make locate a node with low cost and give precise results. In our contribution, we investigate the source of imprecision in the distance vectorhop (DVHOP) localization algorithm. However, we found the last step of DVHOP caused an imprecision in the calculation. Consequently, our work was to replace this step, aiming to reach satisfactory precision. For that purpose, we created three improved versions of this algorithm by adopting two meta-heuristic (simulated annealing, particle swarm optimization) and Fmincon solver dedicated to optimization in the field of WSN node localization. The experimental results obtained in this work prove the efficiency of simulated annealing (SA)-DVHOP in terms of accuracy. Furthermore, the enhanced algorithm outperforms its opponents by varying the percentage of anchors and the number of nodes.

Co-Authors , Alimuddin . Hermawan . Sukenda . Suriansyah . Syafiuddin . Tarsim A M Tahya A S Mubarak A. Oman Sudrajat A. Shofy Mubarak A. Yunianti Aarab, Zineb Ade Sunarma Ade Sunarma Ade Sunarma, Ade Adharto Utiah Agus O . Sudradjat Agus Oman Sudrajat Aldilla Kusumawardhani, Aldilla Alimuddin Alimuddin . Alimuddin Alimuddin Alimuddin Alimuddin alimuddin alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti ANNA OCTAVERA Anouar Darif Aprelia Martina Tomasoa, Aprelia Martina Arroub, Omar Asep Sopian Avarre, Jean-Christophe Azis Azis Bambang Purwantara Benny Heltonika Bethsy J. Pattiasina Cahyono, Tatak Dwi D. Nurlestiyoningrum D.S. Sjafei Damiana Rita Ekastuti DEDI JUSADI Dian Hardiantho Dian Hardianto Didik Ariyanto Dinamella Wahjuningrum Dinar Tri Soelistyowati Dita Puji Laksana Dwi Hany Yanti E. Riani Eddy Supriyono Enang Harris Surawidjaja Etty Riani Fajar Basuki Fajar Maulana . Fauzan, Agung Luthfi Flandrianto S. Palimirmo GORO YOSHIZAKI H. Arfah Hamsah Hamsah Harton Arfah I Khasani I MADE ARTIKA I Wayan Nurjaya I. Herviani I. Mokoginta I. Supriatna Iis Diatin Ikhsan Khasani Imron Imron, Imron Irma Andriani IRMA ANDRIYANI Irvan Faizal Irzal Effendi Iskandar, Andri Ita Djuwita Jamaluddin Jompa Jasmanindar, Yudiana Jean-Christophe Avarre Julie Ekasari K Nisaa K. Sumantadinata Kadarusman . Khairun Nissa Komar Sumantadinata Kukuh Adiyana Kukuh Nirmala Kusman Sumawidjaja Laurent Pouyaud Livana Dethris Rawung Lolita Thesiana M Yamin M. Bintang M. M. Raswin M. Raswin M. Sakdiah M. Syukur M. Toelihere M. Tri Djoko Sunarno M.M. Raswin Meilisza, Nina Mia Setiawati Mozes R . Toelihere MOZES R. TOELIHERE MUHAMMAD AGUS SUPRAYUDI Muhammad Muhammad Muhammad Safir Mulyasari . Mulyasari Mulyasari MUNTI YUHANA Muslim Muslim N Farizah N. Potalangi N.B.P Utomo Nababan, Yanti Inneke Nasrullah, Hasan Naufal, Muhammad Restya Neviaty P. Zamani Nugraha, Media Fitri Isma Nunak Nafiqoh Nur Bambang Priyo Utomo Nurbariah Nurbariah O. Carman Odang Carman Odilia Rovara Poppy Dea Bertha, Poppy Dea Prassetyo Dwi Dhany Wijaya Purnamawati Purnamawati Putri Zulfania, Putri R. Affandi R.G. Pahlawan R.K. Sari R.R Sri Pudji R.R.S.P.S. Dewi Rahmani, My Driss Rahminiwati, Min Rakhmawati, Rakhmawati, RIDWAN AFFANDI Robin . Roza Elvyra S. Darwisito S. Dewi S. Handayani S. Mulyati S. Purwati Safira Qisthina Ayuningtyas, Safira Qisthina Siti Subaidah Sri Nuryati Sri Nuryati Srihadi Agungpriyono Suci Antoro Sugeng Budiharsono Sujaka Nugraha Sukenda . Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Syafyudin Yusuf T.L. Yusuf Tarsim Tarsim TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Triayu Rahmadiah Tuty L . Yusuf W. Manalu Wahyu Pamungkas Wasmen Manalu WIDANARNI WIDANARNI Wisriati Lasima Wiyoto Wiyoto Y. Hadiroseyani Y. Yustikasari Yogi Himawan Yudha Trinoegraha Adiputra Yusnarti Yus Zahri, Abdul

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