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All Journal HAYATI Journal of Biosciences Jurnal Akuakultur Indonesia Jurnal Veteriner Jurnal Perikanan Kelautan Jurnal Natur Indonesia Journal of Aquaculture Management and Technology Jurnal Ilmu-Ilmu Perikanan dan Budidaya Perairan EDULITE: Journal of English Education, Literature and Culture Omni-Akuatika Jurnal Perikanan Universitas Gadjah Mada Indonesian Aquaculture Journal Jurnal Riset Akuakultur Media Akuakultur Creative Research Journal Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Journal of Aquaculture and Fish Health Jurnal Kridatama Sains dan Teknologi Jurnal Magister Pendidikan Matematika (JUMADIKA) Jurnal Abdi Insani Jurnal Ilmiah MEA (Manajemen, Ekonomi, dan Akuntansi) Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat Jurnal Terapis Gigi dan Mulut Jurnal Karya Generasi Sehat Journal of Fisheries & Marine JPEMAS: Jurnal Pengabdian Kepada Masyarakat Jurnal Ilmu Kesehatan

Sri Nuryati

Bogor Agricultural University

Author-ID : 336092

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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The evaluation of glutamine supplementation into the diet on the growth performance, intestinal structure and function of striped catfish Pangasius hypophthalmus (Sauvage, 1878) fry Uttari Dewi; Dedi Jusadi; Mia Setiawati; Sri Nuryati
Jurnal Iktiologi Indonesia Vol 22 No 1 (2022): February 2022
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v22i1.598

A triplicate experiment was conducted to evaluate the effect of glutamine supplementation in the diet on the structure and function of intestine and the growth performance of striped catfish Pangasius hypopthalmus. Forty fishes with an initial body weight of 0.82 ± 0.01 g were distributed into a rectangular aquarium with a size of 60×50×40 cm and a water volume of 70 L. Fish were fed on the diet supplemented with glutamine of either 0, 1, 2, or 3%, respectively. Fish were fed on the diet at satiation for 60 days. Results showed that fish fed on the diet supplemented with 1, and 2% of glutamine significantly had the highest growth performance, including specific growth rate, protein retention, and feed efficiency. On the other hand, feeding on a diet supplemented with 3% of glutamine did not significantly affect the growth performance of fish. Fish in 1% and 2% glutamine treatments had better structure and function of intestine than two other groups of fish, namely the ratio of intestine length with body length, villus surface area, and protease enzyme activity. Thus, supplementation of 1% and 2% glutamine in the diet improves the structure and function of intestine and the growth performance of striped catfish. Abstrak Percobaan dilakukan untuk mengevaluasi pengaruh penambahan glutamin pada pakan terhadap struktur dan fungsi usus, serta kinerja pertumbuhan ikan patin Pangasius hypopthalmus. Ikan uji yang digunakan sebanyak 40 ekor dengan bobot awal 0,82 ± 0,01 g dipelihara dalam akuarium berukuran 60 × 50 × 40 cm dan volume air 70 L. Ikan diberi pakan uji dengan penambahan glutamin 0, 1, 2, dan 3%. Ikan diberi pakan uji secara at satiation selama 60 hari. Hasil penelitian menunjukkan bahwa ikan yang diberi pakan dengan penambahan glutamin 1% dan 2% secara signifikan menghasilkan kinerja pertumbuhan tertinggi, termasuk laju pertumbuhan spesifik, retensi protein, dan efisiensi pakan. Di sisi lain, pakan yang ditambah dengan glutamin 3% tidak signifikan memengaruhi kinerja pertumbuhan ikan. Ikan uji pada perlakuan glutamin 1% dan 2% menghasilkan struktur dan fungsi usus yang lebih baik dibanding perlakuan lainnya, yakni rasio panjang usus dengan panjang tubuh, luas permukaan vili dan aktivitas enzim protease. Disimpulkan bahwa penambahan glutamin 1% dan 2% pada pakan meningkatkan struktur dan fungsi usus serta kinerja pertumbuhan benih ikan patin.

Specific Immune Response Kinetics and Mortality Patterns of Tilapia Oreochromis niloticus on Post-Cocktail Vaccination Period against the Infection of Aeromonas hydrophila and Streptococcus agalactiae Sukenda Sukenda; Tuti Sumiati; Sri Nuryati; Angela Mariana Lusiastuti; Dendi Hidayatullah
Journal Omni-Akuatika Vol 13, No 2 (2017): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Fish vaccination aims to induce a specific immune response indicated by an increase of antibodies in vaccinated fish. However, in accordance with time the presence of will continue to decline. The purpose of this study was to determine the kinetics of specifik immune response and trend mortality against Aeromonas hydrophila and Streptococcus agalactiae on tilapia following vaccination with cocktail vaccine. Fish vaccinated through immersion for 30 minutes in a solution of diluted vaccine. Challenge test was performed for three periods, on day 22, 50, and 78 post-vaccination, fish were challenged with single infection of A. hydrophila 108 cfu. mL-1 and S. agalactiae 104 cfu. mL-1 and co-infection of both bacteria by intraperitoneal. During rearing, the blood fish were taken for determining of serum antibodies, and its measured by ELISA. The results showed that the concentration of specific antibodies vaccinated fish were significantly higher than the control. The basal antibody levels of A. hydrophila before vaccination were higher than S. agalactiae with OD of 0.104 and 0.069 respectively. The maximum antibody response was reached within 70 days of the A. hydrophila OD= 0.264 and 56 days against S. agalactiae OD= 0.188. The mortality rate in the control group was significantly higher than vaccinated on all types and each challenge test period. The trend of mortality due to a single infection of A. hydrophila and co-infections occur more quickly than by S. agalactiae. Lowest mortality occurred in the vaccinated group at 50 day tested challenge.Keywords: kinetics antibody, Aeromnas hydrophila, Streptococcus agalactiae, Oreochromis niloticus

VIRULENCE GENE PROFILING AND PATHOGENICITY OF Streptococcus agalactiae ISOLATED FROM TILAPIA, Oreochromis niloticus FARMS IN INDONESIA Sukenda Sukenda; Achmad Suhermanto; Muhammad Zairin Jr.; Angela Mariana Lusiastuti; Sri Nuryati; Dendi Hidayatullah
Indonesian Aquaculture Journal Vol 16, No 2 (2021): (December, 2021)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.16.2.2021.119-125

Streptococcosis caused by Streptococcus agalactiae has become a major disease problem in tilapia culture in Indonesia. This study aimed to detect virulence genes of S. agalactiae during streptococcosis disease outbreaks in several tilapia farms in Indonesia and evaluate the correlation between biotype and virulence genes to bacterial virulence. The presence of virulence genes was determined in 10 strains of S. agalactiae isolated from farm-raised tilapia. Polymerase chain reaction (PCR) protocol was used to determine genes for cylE, hylB, lmb, bib A, PI-2b, fbs A, fbs B, gap, PI-1, and cfb in the template DNA. Pathogenicity test was carried out by intraperitoneal injection of 24 hour-cultured S. agalactiae to tilapia with 108 CFU/fish. Four isolates have seven of virulence genes (cylE, hylB, bibA, PI-2b, fbs A, fbs B, and gap genes), three isolates have six virulence genes (hylB, bib A, fbs A, fbs B, gap, cfb genes), one isolate has four virulence gene (hyl B, bib A, fbs, and cfb genes), and one isolate has one virulence gene (PI-2b gene). None of the isolates has lmb or PI-1 genes. Bacteria with more virulence genes showed higher pathogenicity post injection. Mortality of tilapia injected with b-hemolytic bacteria was 100% within the period of 14-19 hours, while non-hemolytic bacteria was 53.3%-86.6% on 14 days post-injection. Pathological changes associated with the infection by either isolate included melanosis, slow response, anorexia, ocular opacity, gasping, erratic, C-shape, and whirling. It can be concluded that S. agalactiae with more virulence genes show a higher level of pathogenicity. The presence of a virulent gene has the potential to be used as a basis for selecting candidate isolates and designing vaccine compositions as an effort to prevent streptococcosis infection in tilapia in Indonesia.

IMMUNE RELATED GENES EXPRESSION ANALYSIS IN KOI FISH AFTER VACCINATED WITH KOI HERPES VIRUS DNA VACCINES Sri Nuryati; Fauzan Wahib Alsani; Hasan Nasrullah; Odang Carman; Yuni Puji Hastuti; Eni Kusrini; Alimuddin Alimuddin
Indonesian Aquaculture Journal Vol 15, No 1 (2020): (June, 2020)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.15.1.2020.25-32

Vaccination is a practical step in preventing diseases caused by koi herpes virus (KHV) in koi fish (Cyprinus carpio haematopterus). We have developed two DNA vaccines for KHV named as GP-25 and GP-11 from two local isolates coded as ORF25 and ORF81, respectively. Although both vaccines have been reported to increase survival rates, the evaluation of koi fish immune responses at the molecular level has not been done post-vaccinations. The aim of this research was to determine the effects of koi herpesvirus DNA vaccine on the immune-modulation of koi fish at mRNA level. This recent research used the best vaccine doses of both vaccines determined from our previous study: 7.5 and 12.5 µg per 100 g fish of GP-11, and 12.5 µg per 100 g fish of GP-25. The immune gene expression was analyzed using the RT-qPCR method from the fish liver at 0, 1, 7, 14, and 28 days post-vaccination (dpv). The results showed that, in the vaccinated fish, the immune genes viz. tumor necrosis factor a (TNFa), interleukin-1b (IL1b), interferon-g (IFNg), Mx1, immunoglobulin Mu chain (IgM), and major histocompatibility complex (MHC) class I and class II were induced to significant extents. The higher dose vaccination using the GP-11 vaccine showed higher immune gene expression than that of the lower dose. Furthermore, the GP-25 vaccine had induced lower immune responses than the GP-11 vaccine when using the same dose of vaccination, but relatively the same when the half-dose of GP-11 vaccine was used. In conclusion, the GP-11 and GP-25 vaccine provided the immune-modulatory effects on the koi fish immune response after vaccination.

EFFICACY OF GP-11 KHV DNA VACCINE IN Cyprinus carpio koi Sekar Ayu Chairunnisa; Sri Nuryati; Alimuddin Alimuddin; Sri Murtini; Ayi Santika; Dwi Hany Yanti
Indonesian Aquaculture Journal Vol 11, No 1 (2016): (June 2016)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Koi herpesvirus (KHV) is one of the major pathogen for koi and common carp which cause high mortality and economic losses for the farmer. The purpose of this study was to determine the efficacy of glycoprotein-11 (GP-11) KHV DNA vaccine and compared to GP-25 KHV DNA vaccine. The vaccine in the form of naked DNA plasmidwas delivered by intramuscularly injection to the 3-month-old koi. The fish were divided into six groups, i.e. unvaccinated group (negative control C- and positive control C+), and vaccinated group (2.5 μg/100 μl of GP-11 (group 1), 7.5 μg/100 μl of GP-11 (group 2), 12.5 μg/100 μl of GP-11 (group 3), and 12.5 μg/100 μl of GP-25 (group 4)). At day 42 post vaccination, all fish of each groups were challenged by injecting KHV titre 10-3 FID50. Number of dead fish was counted everyday after the challenge until 30 days. The results showed that vaccinated fish were had survival rate of 83.33-93.33% (group 2, 3 and 4). It’s show that GP-11 KHV DNA vaccine has high efficacy. As conclusion, GP-11 DNA vaccine could be an alternative DNA vaccine for preventing KHV infection.

RESPONS FISIOLOGIS DAN KINERJA PERTUMBUHAN IKAN NILA PADA MEDIA RENDAH AMONIA DAN DIBERI SUPLEMEN ASAM GLUTAMAT Titin Kurniasih; Dedi Jusadi; Muhammad Agus Suprayudi; Sri Nuryati; Muhammad Zairin Jr.; Eddy Supriyono
Jurnal Riset Akuakultur Vol 15, No 3 (2020): (September, 2020)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Ketika dipapar media tinggi amonia, ikan nila mengalami perubahan metabolisme asam amino yang cukup signifikan, dan suplementasi asam glutamat berguna untuk memperbaiki perubahan yang merugikan akibat paparan amonia. Akan tetapi informasi mengenai aspek metabolisme asam amino pada ikan nila yang dipapar amonia rendah masih sangat terbatas. Penelitian ini dilakukan untuk mengevaluasi pengaruh suplementasi asam glutamat pada ikan nila merah yang dipelihara pada media budidaya rendah amonia terhadap respons fisiologis dan kinerja pertumbuhan. Ikan nila dengan bobot rata-rata 9,97 ± 0,38 g ditebar sebanyak 20 ekor pada setiap akuarium (padat tebar 1,0 g L-1). Empat jenis pakan isoprotein (kadar protein 28%) dan isoenergi (4245 ± 22,48 kkal kg-1) disuplementasi asam glutamat masing-masing sebanyak 0% (Glu 0), 0,75% (Glu 0,75), 1,5% (Glu 1,5) dan 2,25% (Glu 2,25). Setiap perlakuan diberi empat ulangan. Penelitian ini dilakukan selama 60 hari. Hasil penelitian menunjukkan bahwa pemberian pakan yang ditambah glutamat memberi efek pada respon fisiologis ikan. Aktivitas enzim aspartate aminotransferase (AST) pada Glu 2,25 lebih rendah dibandingkan dengan perlakuan lainnya, yang menjadi indikasi penurunan beban kerja hati. Ada kecenderungan peningkatan kadar aspartat, alanin, leusin, isoleusin dan valin pada jaringan hati seiring dengan meningkatnya kadar suplementasi asam glutamat. Di dalam penelitian ini, kinerja pertumbuhan ikan tidak berbeda nyata antar perlakuan. Kesimpulannya adalah bahwa konsumsi pakan yang ditambah asam glutamat 2,25% mampu memperbaiki respons fisiologis ikan akibat menurunnya beban kerja hati yang dicirikan dengan penurunan nilai AST, serta meningkatnya kandungan beberapa asam amino hati, walau belum mampu memperbaiki kinerja pertumbuhan dan pemanfaatan pakan oleh ikan nila. Informasi ini berguna untuk pengembangan riset terkait aspek metabolisme asam amino pada ikan nila yang terpapar media tinggi amonia.When exposed to high ammonia aquatic environment, nile tilapia experienced a significant change in hepatic amino acid metabolism and glutamic acid supplementation can reduce the effects of the adverse change. However, there are no sufficient information on the amino acid metabolisme of tilapia exposed to low environmental ammonia. This research was performed to evaluate the effects of oral supplementation of glutamic acid on the aminotransferase enzymes activity and growth performance of red tilapia reared in low environmental ammonia (LEA) with NH4 concentration of 0.10 mg L-1. Fish with an average weight of 9.97 ± 0.38 g were stocked with an initial rearing density of 1.0 g L-1(20 fish in each aquarium). Four isonitrogenous (crude protein 28%) and isocaloric (4246 ± 22.48kcal kg-1) experimental diets were prepared with supplementation of different ratios of glutamic acid at 0% (Glu0), 0.75% (Glu0.75), 1.5%(Glu1.5) and 2.25 % (Glu2.25) to feed, respectively. All treatment groups were arranged quadruplicate. Fish were fed with the diets for 60 days. The results showed that the supplementation of glutamic acid in the diet affected the physiological response of the fish. The aspartate aminotransferase (AST) activity of Glu2.25 was significantly lower compared to that of the other treatments, which indicated a decrease in liver workload. There is a tendency of increased levels of hepatic free aspartate, alanine, leucine, isoleucine, and valine following the increase of glutamic acid supplementation level. The fish growth performance was insignificantly different between the treatments. It is concluded that a diet supplemented with 2.25% of glutamic acid could improve the physiological response of red tilapia, although no significant growth improvement should be expected. These research finding could serve as an important basic information for future research on amino acid and endogenous ammonia metabolism in nile tilapia exposed to high ammonia aquatic environment.

STATUS KESEHATAN IKAN SIDAT (Anguilla sp.) PADA PERAIRAN UMUM DAN WADAH PEMELIHARAAN SEMENTARA Agung Cahyo Setyawan; Sukenda Sukenda; Sri Nuryati
Jurnal Riset Akuakultur Vol 10, No 1 (2015): (Maret 2015)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Status kesehatan ikan sidat (Anguilla sp.) telah dianalisis untuk menunjukkan terjadinya penurunan stok karena infeksi patogen dan penangkapan benih berlebihan untuk budidaya. Di Indonesia, ketiadaan standar penangkapan, pemeliharaan dan budidaya menyebabkan terjadinya penurunan kualitas dan kuantitas produksi ikan sidat. Penelitian ini dilakukan untuk mengevaluasi status kesehatan ikan sidat di perairan Indonesia dengan sampel dari Kabupaten Banyumas dan Cilacap, Jawa Tengah. Sampling dilakukan pada awal musim penghujan, yaitu bulan September hingga November 2012. Sebanyak 113 ekor ikan sidat ditangkap dan dianalisis dalam penelitian ini, dengan perincian 57 ekor diamati langsung setelah ditangkap dan 56 ekor diamati setelah dipelihara selama 10 hari oleh pengepul. Pengamatan dilakukan dengan metode observasi langsung menggunakan mikroskop untuk parasit, kit API 20NE (Biomeureux®) untuk bakteri dan pewarnaan standar haematoxylin-eosin untuk histopatologi. Terdapat empat jenis parasit (Nematoda: Camallanidae dan Anguillicoloides; Platyhelminthes: Monogenea dan Digenea) dan lima bakteri (Aeromonas hydrophylla, Pseudomonas luteola, Vibrio fluvialis, Aeromonas sobria, dan Aeromonas caviae) dari sampel ikan sidat dalam penelitian ini. Tidak terdapat perbedaan dalam komposisi patogen, namun terjadi perubahan dalam kondisi histopatologi sehingga pemeliharaan sementara oleh pengepul sebelum ikan sidat dibudidayakan memiliki potensi menurunkan kualitas benih untuk budidaya.

TOKSISITAS SEL UTUH DAN EXTRACELLULAR PRODUCT (ECP) Streptococcus agalactiae β-HEMOLITIK DAN NON-HEMOLITIK PADA IKAN NILA (Oreochromis niloticus) Achmad Suhermanto; Sukenda Sukenda; Muhammad Zairin Jr.; Angela Mariana Lusiastuti; Sri Nuryati
Jurnal Riset Akuakultur Vol 13, No 4 (2018): (Desember 2018)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Bakteri Streptococcus agalactiae tipe β-hemolitik dan non-hemolitik menjadi agen penyebab infeksi streptococcosis yang mengakibatkan kematian dan kerugian besar pada budidaya ikan nila. Penelitian ini bertujuan untuk membandingkan toksisitas sel utuh dan extracellular product (ECP) bakteri b-hemolitik dan non-hemolitik yang diinjeksikan pada ikan nila. Karakterisasi S. agalactiae berdasarkan SNI dan API 20 STREP, serta pemisahan protein dengan metode SDS-PAGE. Pengujian toksisitas dilakukan dengan cara menginjeksikan sel utuh dan ECP S. agalactiae secara intraperitoneal (IP) dengan dosis 0,1 mL ekor-1. Hasil uji biokimia, dan konfirmasi dengan API 20 STREP menunjukkan bahwa semua isolat positif S. agalactiae. Fraksinasi protein pada sel utuh bakteri diperoleh pita protein masing-masing sebanyak sembilan dan tujuh pita pada tipe β-hemolitik dan non-hemolitik. Fraksinasi ECP teridentifikasi pada β-hemolitik sebanyak tujuh pita dan non-hemolitik empat pita protein. Konsentrasi protein sel utuh dan ECP b-hemolitik lebih besar dibandingkan bakteri non-hemolitik. Gejala abnormalitas lebih cepat terjadi pada ikan nila yang diinjeksi ECP bakteri b-hemolitik dan berbanding lurus dengan kematian sebanyak 91%-100% pada jam ke-13 pascainjeksi. Hasil ini menunjukkan bahwa ECP bakteri S. agalactiae β-hemolitik lebih virulen dibandingkan tipe non-hemolitik. Hingga akhir pemeliharaan tidak ada kematian pada ikan yang diinjeksi sel utuh bakteri S. agalactiae b-hemolitik dan non-hemolitik. Studi histopatologi ikan yang diinjeksi ECP S. agalactiae pada organ hati, limpa, otak, dan ginjal menunjukkan adanya kongesti, hemoragi, dan nekrosis.The β-hemolytic and non-hemolytic biotype of Streptococcus agalactiae are the agents that cause streptococcosis infection which resulted in high mortality and major losses in tilapia culture. This study aimed to compare the toxicity of whole cell and extracellular product (ECP) b-hemolytic and non-hemolytic bacteria from injected tilapia. Characterization of S. agalactiae was based on SNI and API 20 STREP and protein separation by SDS-PAGE method. Toxicity test was carried out by injecting whole cells and ECP S. agalactiae intraperitoneally with a dose of 0.1 mL fish-1. The results of biochemical tests, with confirmation by API 20 STREP showed that all isolates were positive for S. agalactiae. Protein fractionation of whole bacterial cells obtained as many as nine and seven bands of protein in b-hemolytic and non hemolytic biotype, respectively. ECP fractionation was identified in β-hemolytic biotype as many as seven bands and four protein bands in non-hemolytic. The whole cell protein concentration and ECP β-hemolytic were higher than non-hemolytic bacteria. Symptoms of abnormalities occurred faster in tilapia which was injected with ECP b-hemolytic bacteria and had positive correlation with 91%-100% mortalities at the 13th hours post-injection. This results indicated that ECP of S. agalactiae β-hemolytic are more virulent than non-hemolytic. Until the end of the trial, there were no deaths in fish injected with whole cells of b-hemolytic and non-hemolytic S. agalactiae. Histopathological studies of ECP-injected fish S. agalactiae in the liver, spleen, brain, and kidneys showed congestion, hemorrhage, and necrosis.

PENGEMBANGAN METODE ELISA UNTUK MENDETEKSI RESPON IMUN SPESIFIK PADA IKAN NILA (Oreochromis niloticus) YANG DIVAKSINASI TERHADAP Aeromonas hydrophila DAN Streptococcus agalactiae Tuti Sumiati; Sukenda Sukenda; Sri Nuryati; Angela Mariana Lusiastuti
Jurnal Riset Akuakultur Vol 10, No 2 (2015): (Juni 2015)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Ko-infeksi bakteri Aeromonas hydrophila dan Streptococcus agalactiae rentan terjadi pada budidaya nila, sehingga pencegahan menggunakan vaksin koktail yang mengandung kedua bakteri tersebut merupakan langkah yang tepat untuk pengendaliannya. Penelitian ini bertujuan untuk menganalisa respon imun spesifik (antibodi) serum ikan nila yang divaksinasi dengan vaksin koktail A. hydrophila dan S. agalactiae dengan menggunakan ELISA. Penelitian dilakukan dalam dua tahap, tahap pertama adalah optimalisasi ELISA, yang terdiri atas persiapan antigen dan penentuan konsentrasi optimum antigen untuk deteksi antibodi dan tahap kedua adalah mendeteksi antibodi pasca vaksinasi ikan nila. Hasil penelitian menunjukkan bahwa konsentrasi optimum antigen A. hydrophila dan S. agalactiae untuk penentuan antibodi dalam ikan nila masing-masing sebesar 10 μg/mL. Konsentrasi tersebut dapat digunakan untuk mendeteksi antibodi serum pada ikan nila setelah diberi vaksin koktail A. hydrophila dan S. agalactiae. Titer antibodi ikan nila pada minggu kedua sampai pada minggu kelima pada kelompok vaksinasi secara signifikan lebih tinggi dibanding kontrol (P<0,05).

PEMBERIAN VAKSIN DNA ANTI-KHV IKAN MAS DENGAN DOSIS BERBEDA TERHADAP BENIH IKAN KOI Eni Kusrini; Sri Nuryati; Siti Zubaidah; Lili Sholihah
Jurnal Riset Akuakultur Vol 14, No 2 (2019): (Juni, 2019)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Salah satu upaya penanggulangan wabah koi herpes virus (KHV) yang biasa menyerang ikan koi dan ikan mas adalah dengan pemberian vaksin DNA. Vaksin DNA dapat merangsang kekebalan spesifik dan kekebalan yang ditimbulkan relatif tinggi, serta aman digunakan. Penelitian ini bertujuan menguji beberapa dosis vaksin DNA terhadap benih ikan koi sehingga didapatkan dosis yang tepat yang dapat memberikan RPS (relative percent survival) dan imunitas terbaik. Perlakuan yang diberikan adalah vaksinasi dengan dosis berbeda melalui teknik injeksi secara intra muskular, yaitu perlakuan A (7,5 µg/100 µL), B (10 µg/100 µL), C (12,5 µg/100 µL), serta kontrol positif (ikan tidak divaksinasi tetapi diuji tantang) dan kontrol negatif (ikan tidak divaksinasi dan tidak diuji tantang). Hasil dari penelitian ini menunjukkan bahwa pemberian vaksin dengan dosis berbeda mampu meningkatkan sintasan pada ikan koi, serta vaksin DNA dengan dosis 12,5 µg/100 µL mampu memberikan nilai sintasan ikan koi yang diinfeksi KHV sebesar 97,22% dan memberikan nilai RPS sebesar 95,83 ± 0,58%; serta memberikan gambaran sistem imun terbaik dibandingkan perlakuan lainnya.One of the alternatives to deal with the koi herpesvirus (KHV) infections in koi fish and common carp is through the use of DNA vaccine. DNA vaccines can stimulate a high level of specific immune responses and induced immunity and are relatively safe to be used. This research was aimed to determine the effective dose of DNA vaccine for the best relative percent survival (RPS) and immunity. The treatments consisted of different vaccine doses administered through intramuscular injection: treatment A (7.5 mg/100 mL), B (10 mg/100 mL), C (12.5 mg/100 mL), and the positive control (non-vaccinated fish but challenged by KHV test) and negative control (non-vaccinated fish and not challenged by KHV test). The results showed that the administration of the vaccine with different doses had increased the survival of koi fish across all treatments (excluding positive and negative controls). Vaccine dose of 12.5 mg/100 mL was considered as the best vaccine dose. The vaccine dose had produced the best survival value of 97.22% and resulted in the highest RPS values of 95.83 ± 0.58%. DNA vaccine is expected to be the future vaccine.

Co-Authors , Alimuddin , Rahman, , , Rusli, , , Sofian, , , Yuliyanti . Kurdianto . Taukhid A. Santika A. Suwanto Aditya, Tiya Widi Afif Abdurrahman Afiff , Usamah Agung Cahyo Setyawan Agus Oman Sudrajat Agussalim, Anita Mardiana Ahmad Beni Rouf Ahmad Firdaus, Ahmad Ahmad Muzaki Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin amperawati, metty Amperawati2, Metty Andi Tiara Eka Diana Puteri, Andi Tiara Eka Diana Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Anis Nugrahawati Anita Setyowati Srie Gunarti Ardana Kurniaji Asrido, Farhan Astu Rahman, Waljuni Ayi Santika Ayi Santika Ayu Dhita Juliadiningtyas, Ayu Dhita Bagus Rahmat Basuki Brite, Margie BUNGA NURWATI D. Meha D. Puspitaningtyas D. Wahjuningrum Dadi, Oswaldus Danan, Danan DEDI JUSADI Dendi Hidayatullah Dendi Hidayatullah Dendi Hidayatullah Dendi Hidayatullah, Dendi Dian Febriani Dian Hardiantho Dinamella Wahjuningrum Dinar Tri Soelistyowati Dwi Hany Yanti Dwi Hany Yanti Eddy Supriyono Eko Darma Elma yulius Elman Efzi, Agno Eni Kusrini Eni Kusrini Erni Susanti F.B.P. Sari F.H. Pasaribu Fachriyan Hasmi Pasaribu Fajar Prihesnanto, Fajar Fajrin, Anang Falah, Fazrul Faoziyatunnisa, Nurul Fateah, Nur Fauzan Wahib Alsani Firdausi, Amalia Putri Firmansyah, Arif Lukman Fitriyah Husnul Khotimah Fitriyah Husnul Khotimah Goro Yoshizaki Gusti Ngurah Permana Habibah, Siti Sab'atul Habibah, Siti sabatul Hanggara, Yudha Harton Arfah Haryanti Haryanti Haryanti Haryanti Hasan Nasrullah Hidayah, Winda Alfina Ibnu Bangkit Bioshina Suryadi IDA RAHMAWATI Iis Widiani Ikhsan Khasani Indah Mastuti ISNAWATI Isnawati Isnawati Isni Rahmatika Sari Julie Ekasari K. Sumantadinata Ketut Mahardika Ketut Sugama Ketut Sugama Kisworo Utami, Naning Komar Sumantadinata Kukuh Nirmala L.S. Harti La Ode Muhammad Arsal Lili Sholihah Lilis Nurjanah M. Zairin Junior M.A. Suparman Mia Setiawati Mira Mawardi Muchtar, Muthahharah MUHAMMAD AGUS SUPRAYUDI Muhammad Zairin Jr. Muhammad Zairin Jr. Muhammad Zairin Jr. Mukni Mukni, Mukni Mulyani, Rahma Munawarah, Husnadatul MUNTI YUHANA N. Ashry N.A. Maswan Nababan, Yanti Inneke Nadia Aulia, Nadia Nasrullah, Hasan Ninik Paryati Nisa, Hilwatun Nugroho, Sigit Dani Nur Rahmawati Nurbariah Nurbariah Nurhasanah Nurina Pratiwi Nurly Faridah Nurly Faridah NURWATI, BUNGA Odang Carman P. Giri P. Hariyadi Priyoutomo, Nur Bambang Puteri, Rafilah Putri, Elva Khairina Qorie Astria R.D. Soejoedono Raharjo, Pamuji Gesang Rahma Mulyani Rahmadiniyah, Rahmadiniyah Rahman Rahman, Waljuni Astu Rahmi, Kurnia Anggraini Rahminiwati, Min Ramadhan, Afriani Ramadhani, Danika Athirah Retno Damayanti Soejoedono S.H. Sholeh Said, Fahmi Sari Anggraeni, Sukma Sari Budi Moria Sembiring Sari Budi Moria Sembiring Sarjito - SATRIYAS ILYAS Sekar Ayu Chairunnisa Sekar Ayu Chairunnisa Sekar Sulistyaning Hadiwibowo Septia Armayani, Dilla Siti Murniasih Siti Salamah Siti Zubaidah Slamet Budi Prayitno Soko Nuswantoro sri murtini . Suhermanto, Achmad Sukenda . Sukenda . Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda, Sukenda, Suryani, Dessy Rizki Tiara Puspa Anjani Tira Silvianti Titin Kurniasih Trian Rizky Febriansyah Tuti Sumiati Tuti Sumiati Utami, Naning K. Utomo, Nurbambang Priyo Uttari Dewi Wahyuwardani , Sutiastuti Wasmen Manalu Wida Lesmanawati WIDANARNI WIDANARNI Y. Hadiroseyani Y. Kuswardani Yanti, Mauliza Yuni Puji Hastuti Yusril Pratama, Muhammad Zakki Zainun Zulhelmi, Arif

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