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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Agrotrop : Journal on Agriculture Science Zuriat Jurnal Natur Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Agricultural Science Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Jurnal Tanaman Industri dan Penyegar AGRIVITA, Journal of Agricultural Science Jurnal Agroekoteknologi Jurnal Aplikasi Bisnis dan Manajemen (JABM) E-Journal Widyariset Planta Tropika JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Biologi Tropis ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education Journal of Tropical Crop Science JOURNAL OF SCIENCE AND SOCIAL RESEARCH axial : jurnal rekayasa dan manajemen konstruksi Menara Perkebunan Sainstek : Jurnal Sains dan Teknologi CORD Jurnal Penelitian Tanaman Industri (Littri) Buletin Palma Widyariset Journal Of Sustainability Perspectives Journal of Sustainability Perspectives Buitenzorg: Journal of Tropical Science Annales Bogorienses
Agus Purwito
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian IPB Jalan Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia
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Journal : Jurnal AgroBiogen

 1 
Efficacy of RB gene in transgenic potato Katahdin SP904 and SP951 to West Java isolates of Phytophthora infestans Ambarwati, A. Dinar; Sumaraw, S. M.; Purwito, Agus; Herman, M.; Suryaningsih, E.; Aswidinnoor, Hajrial
Jurnal AgroBiogen Vol 7, No 1 (2011): Jurnal AgroBiogen
Publisher : Jurnal AgroBiogen

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Potato late blight, caused by Phytophthora infestans is one of the most devastating plant disease. Potato yield losses due to this disease ranged from 47-100%. A major late blight resistance gene, called RB, previously was identified in the wild potato species Solanum bulbocastanum. RB gene has been integrated into cultivated potato Katahdin using Agrobacterium-mediated transfor-mation, and showed durable and broad spectrum resistance either in laboratory assay or in confined field trial. Evaluation of transgenic Katahdin SP904 and SP951 was conducted to verify whether the RB gene with broad spectrum to all known races of P. infestans in the United States and in Toluca, Mexico was also effective against P. infestans isolates in Indonesia. Efficacy of RB gene was evaluated for foliar and tuber resistance to West Java isolates. Transgenic Katahdin were more resistant in foliar than non transgenic plants, at 14 days after inoculation. Diseases intensity of transgenic Katahdin SP904 and SP951 were 19.8-43.8%, whereas non transgenic Katahdin, Granola, and Atlantic were 46.9-100%. In contrast to the foliar resistance phenotype, RB-containing tubers in transgenic Katahdin did not exhibit increased resistance to Lembang, Pangalengan and Galunggung isolates. Tubers of transgenic Katahdin SP904, SP951, and non transgenic Katahdin showed lesion volume of 0.93, 0.91, and 0.91 cm3, respectively. RB gene in transgenic Katahdin showed efficacy against late blight P. infestans in foliar, but did not showed efficacy in tuber. Transgenic Katahdin RB thus providing a potential source of resistance for breeding programs.
Analisis Integrasi dan Segregasi Gen Ketahanan terhadap Hawar Daun pada Progeni F1 Hasil Persilangan Tanaman Kentang Transgenik dengan Non Transgenik Ambarwati, Alberta Dinar; Purwito, Agus; Herman, Muhamad; Sumaraow, S. M.; Aswidinnoor, Hajrial
Jurnal AgroBiogen Vol 1, No 1 (2005): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v1n1.2005.p%p

Abstract

Potato late blight, caused by Phytophthora infestans is one of the most devastating plant diseases. Potato yield losses due to this disease ranged from 47-100%. Frequent intervals and high rates of fungicide spray, currently practiced by potato growers to control the disease are expensive. Host resistance is an alternative control measure that is more economically and environmentally sustainable. Development of late blight resistant plants was conducted by crossing RB transgenic Katahdin SP904 andSP951 as male and two susceptible (Atlantic, Granola) varieties as female parents. F1 progenies were molecularly characterized for the integration of the RB transgene and evaluated for their segregations. Crossing data of Atlantic x transgenic Katahdin SP904 and SP951 produced 71 (57.72%) berries with average number of seeds per berry 139.58 and 83 (41.29%) berries with average number of seeds/berry 85.23, respectively. Granola x transgenic Katahdin SP904 and SP951 crosses gave higher results in terms of berry set (79.55 and 84.44%, respectively) than Atlantic x transgenic Katahdin crosses. A total of 554 F1 progenies were analyzed for the presence of the RB PCR marker. An expected 619-bp and 840-bp band were amplified in the progenies that contain the RB gene. The RB gene was integrated in 65 (45.45%), 77 (47.83%), 47 (45.63%), and 71 (48.30%) F1 progenies of Atlantic x transgenic Katahdin SP904, Atlantic x transgenic Katahdin SP951, Granola x transgenic Katahdin SP904, andGranola x transgenic Katahdin SP951, respectively. Chisquare tests showed that all the four cross combinations followed a 1 : 1 segregation ratio.
Efikasi Gen RB pada Tanaman Kentang Transgenik Katahdin SP904 dan SP951 terhadap Empat Isolat Phytophthora infestans dari Jawa Barat Ambarwati, Alberta Dinar; Sumaraw, S M; Purwito, Agus; Herman, Muhammad; Suryaningsih, E.; Aswidinnoor, Hajrial
Jurnal AgroBiogen Vol 7, No 1 (2011): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v7n1.2011.p28-36

Abstract

Efficacy of RB gene in transgenic potato Katahdin SP904and SP951 to West Java isolates of Phytophthorainfestans. A. Dinar Ambarwati, S.M. Sumaraw, AgusPurwito, M. Herman, E. Suryaningsih, and HajrialAswidinnoor. Potato late blight, caused by Phytophthorainfestans is one of the most devastating plant disease. Potatoyield losses due to this disease ranged from 47-100%. Amajor late blight resistance gene, called RB, previously wasidentified in the wild potato species Solanumbulbocastanum. RB gene has been integrated into cultivatedpotato Katahdin using Agrobacterium-mediated transformation,and showed durable and broad spectrum resistanceeither in laboratory assay or in confined field trial. Evaluationof transgenic Katahdin SP904 and SP951 was conducted toverify whether the RB gene with broad spectrum to allknown races of P. infestans in the United States and inToluca, Mexico was also effective against P. infestansisolates in Indonesia. Efficacy of RB gene was evaluated forfoliar and tuber resistance to West Java isolates. TransgenicKatahdin were more resistant in foliar than non transgenicplants, at 14 days after inoculation. Diseases intensity oftransgenic Katahdin SP904 and SP951 were 19.8-43.8%,whereas non transgenic Katahdin, Granola, and Atlanticwere 46.9-100%. In contrast to the foliar resistancephenotype, RB-containing tubers in transgenic Katahdin didnot exhibit increased resistance to Lembang, Pangalenganand Galunggung isolates. Tubers of transgenic KatahdinSP904, SP951, and non transgenic Katahdin showed lesionvolume of 0.93, 0.91, and 0.91 cm3, respectively. RB gene intransgenic Katahdin showed efficacy against late blight P.infestans in foliar, but did not showed efficacy in tuber.Transgenic Katahdin RB thus providing a potential source ofresistance for breeding programs.
Perbanyakan dan Konservasi In Vitro Plasma Nutfah Talas (Colocasia esculenta (L.) Schoot) Nurwita Dewi; Bambang S. Purwoko; Ida Hanarida; Agus Purwito; Iswari S. Dewi
Jurnal AgroBiogen Vol 8, No 3 (2012): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n3.2012.p105-112

Abstract

Taro is a potential source of carbohydratefor anticipation of climate change. In vitro technology havenot been widely implemented for tuber crops conservation.Conservation of the crops is mostly conducted in field. Suchconservation is very susceptible to biotic and abiotic stress.The research consisted of two activities i.e: micropropagationand conservation. The objectives were to obtain taro invitro propagation and conservation method. The trial wasarranged in a factorial design with six replications. Five taroaccessions were used as the first factor for each study. Thesecond factor in propagation study was propagation mediumi.e: MS; MS + 2.9 μM IAA + 4.4 μM BA and MS + 2.9 μM IAA+ 22,2 μM BA. Shoot tip from taro sucker was used asexplant. The second factor in conservation study was MSmedium containing mannitol (0, 30, 40, and 50 g/l). Twoleavesin vitro shoots from micropropagation study was usedas explants. The addition of BA in MS medium + 2.9 μM IAAincreased the number of shoot of taro germplasm. The bestmedium for micropropagation of taro germplasm No. 21 andTalas Jahe is MS + 2,9 μM IAA + 4,4 μM BA, whereas thebest medium for No. 503, Talas Jahe and Lumbu Banten isMS + 2,9 μM IAA + 22,2 μM BA. Based on data of plantheight, percentage of leaf life and shelf life, MS medium +manitol 40 g/l was the best medium for taro germplasmconservation with prolong sub-culture interval.
Regenerasi Jeruk Siam melalui Embriogenesis Somatik Ali Husni; Agus Purwito; Ika Mariska; Sudarsono Sudarsono
Jurnal AgroBiogen Vol 6, No 2 (2010): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v6n2.2010.p75-83

Abstract

The Regenerated of Siam Tangerin through SomaticEmbryogenesis. Ali Husni, Agus Purwito, Ika Mariska,and Sudarsono. Somatic embryogenesis occurs in mostplants that are cultured on a suitable medium in vitro.Somatic embryo may arise from single cells and the embryogeniccells are widely applicable in plant propagation,genetic manipulation and transgenic technologies. Thepresent study was carried out to develop an effectivesomatic embryogenesis technique to regenerate Siamtangerine plants. Materials used in this study were nucellartissues of young fruits (30-90 days post anthesis). Inductionof embryogenic calli was done by culturing the tissues onthree different basal media (MS, MW and MT). Embryomaturation was done on the MW medium + ABA (0; 0.1; 0.3;dan 0.5 mg/l), while germination to plantlet developmentwas done on WS medium + GA3 (0, 0.1, 0.3, and 0.5 mg/l).The results showed that among the three media, MW wasthe best medium for callus induction from nucellar tissueswith a success rates 98% for Simadu and 100% forPontianak. The best maturation of embryo somatic wasdone on MW medium + ABA 0.5 mg/l with success rates99%, while the best medium for germination and developmentinto planlets was MW medium + 0.5 mg/l GA3 with asuccess rate 58%.
Analisis Integrasi dan Segregasi Gen Ketahanan terhadap Hawar Daun pada Progeni F1 Hasil Persilangan Tanaman Kentang Transgenik dengan Non Transgenik Alberta Dinar Ambarwati; Agus Purwito; Muhamad Herman; S. M. Sumaraow; Hajrial Aswidinnoor
Jurnal AgroBiogen Vol 5, No 1 (2009): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v5n1.2009.p25-31

Abstract

Potato late blight, caused by Phytophthora infestans is one of the most devastating plant diseases. Potato yield losses due to this disease ranged from 47-100%. Frequent intervals and high rates of fungicide spray, currently practiced by potato growers to control the disease are expensive. Host resistance is an alternative control measure that is more economically and environmentally sustainable. Development of late blight resistant plants was conducted by crossing RB transgenic Katahdin SP904 andSP951 as male and two susceptible (Atlantic, Granola) varieties as female parents. F1 progenies were molecularly characterized for the integration of the RB transgene and evaluated for their segregations. Crossing data of Atlantic x transgenic Katahdin SP904 and SP951 produced 71 (57.72%)berries with average number of seeds per berry 139.58 and 83 (41.29%) berries with average number of seeds/berry 85.23, respectively. Granola x transgenic Katahdin SP904 and SP951 crosses gave higher results in terms of berry set (79.55 and 84.44%, respectively) than Atlantic x transgenic Katahdin crosses. A total of 554 F1 progenies were analyzed for thepresence of the RB PCR marker. An expected 619-bp and 840-bp band were amplified in the progenies that contain the RB gene. The RB gene was integrated in 65 (45.45%), 77 (47.83%), 47 (45.63%), and 71 (48.30%) F1 progenies of Atlantic x transgenic Katahdin SP904, Atlantic x transgenic Katahdin SP951, Granola x transgenic Katahdin SP904, andGranola x transgenic Katahdin SP951, respectively. Chisquare tests showed that all the four cross combinations followed a 1 : 1 segregation ratio.
Co-Authors , Asnawatr , Nurhasanah , Rustikawati , Samanhudi , Suharsono , Supenti . LUKMAN A. Dinar Ambarwati Abimantara, Grandisa Cahya Agus Joko Santoso Agus Joko Santoso Alberta Dinar Ambarwati Alberta Dinar Ambarwati Alberta Dinar Ambarwati Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Alina Akhidaya Ambarwati, Alberta Dinar Ambarwati, Alberta Dinar Amrullah, Rizki Abi Anas D Susila Andri Ernawati Anjani, Imas Galuh Antonius Suwanto Arif Satria Arifin Noor Sugiharto Arifin Noor Sugiharto ARSYAD, MIRZA ARSIATY ARSYAD, MIRZA ARSIATY Asep Setiawan Atra Romeida Awang Maharijaya Bambang S. Purwoko Bambang Sapta Purwoko Branco, Luis Manuel Budi Marwoto Budi Marwoto Budi Winarto Catur Herison Chaireni Martasari Dewi Sukma Dian Puji Rahayu Didy Soepandi Didy Soepandi, Didy Didy Sopandie DINARTI, DINY Dini Dinarti Diny Dinarti DWI ANDREAS SANTOSA Dyah Manohara Dyah Retno Wulandari Dyah Retno Wulandari E. Suryaningsih Endah Retno Palupi Endang Gunawan ENDANG SUHENDANG Enny Sudarmonowati Enny Sudarmonowati Enny Sudarmonowati Eri Sofiari Erni Suminar Fathur Rahmi, Atika Fitri Rachmawati Fitri Yulianti G. A. Wattimena G. A. Wattimenal GA Wattimena GA Wattimena Gustaaf Adolf Wattimena Gustaff Adolf Wattimena Gustav Adolf Wattimena H . M. Machmud HAJRIAL ASWIDINNOOR Hartati, Raden Roro Sri Harti, Heri Herison, Catur Herman, Muhamad Herman, Muhammad I Made Arisudana Putra Ida Hanarida Ika Mariska Ika Mariska Imas Sukaesih Sitanggang Indriati Husain Inez H.S. Loeddin Suharsono Irdika Mansur ISKANDAR ZULKARNAEN SIREGAR ISMAIL MASKROMO Ismail Maskromo Iswari S. Dewi J. K. J. Laisina J. M. Pasaribu Juang Gema Kartika Julius D. Nugroho Kalsum Yulifar, Andi Sri Ummi Kartiman, Roni Kartiman, Roni Karyanti ,, Karyanti Kikin H Mutaqin Komang Trisna Wirakusuma Kosasih, Akhmad KRISTIANTO NUGROHO, KRISTIANTO Laela Sari Laela Sari Laela Sari laela Sari, laela LAREKENG, SITI HALIMAH M. Herman M. Machmud Maharijaya, dan Awang Marlin Marlin MASKROMO, ISMAIL Mastur Mastur Matjik, Nurhayati Anshori MATTJIK, NURHAYATI ANSHORI Megayani Sri Rahayu Memen Surahman Meynarti Sari Dewi Ibrahim Meynarti Sari Dewi Ibrahim Meynarti Sari Dewi Ibrahim Mia Kosmiatin Mia Kosmiatin Mia Kosmiatin Mira Humaira Mohamad Prayogi Muhamad Herman Muhammad Alwi Muhammad Herman MUHAMMAD HERMAN Muhammad Mahmud Muhammad Reza Zakie MUHAMMAD SYUKUR Ni Made Armini Wiendi Noor Farid Nur Laela Wahyuni Meilawati Nurhajati Ansori Mattjik NURHAYATI ANSHORI MATTJIK Nurhayati Ansori Mattjik NURITA TORUAN-MATHIUS Nurliani Bermawie Nurul Khumaida Nurwita Dewi Prasetyawati , Adinda Prima Muklisa PUJI LESTARI Purba, Dumaris Priskila Putra, Heriansyah Putri, Halida Adistya Ragapadmi Purnamaningsih RAGAPADMI PURNAMANINGSIH Ratna Trisnawati Ravenska, Nidya Rd. Selvy Handayani Rd. Zainal Frihadian REFLINUR REFLINUR Retno Prihatini Reza Ramdan Rivai Reza Ramdan Rivai Ridho Kurniati Ridho Kurniati Rizki Abi Amrullah Roedhy Poerwanto Roni Kartiman Roni Kartiman Rr Sri Hartati Rr Sri Hartati, Rr Sri RR. Sri Hartati Rr. Sri Hartati Rr. Sri Hartati Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo S. M. Sumaraow S. M. Sumaraw S. Sudarsono Sari, Dewi Citra Sari, Laela SATRIYAS ILYAS Sembiring, Rinawati Sientje Mandang Sumaraw SIENTJE MANDANG SUMARAW SITI HALIMAH LAREKENG, SITI HALIMAH Slamet Susanto Slamet, Alim Setiawan Sobir Sobir Sri Rianawati Sudarmonowati, Enny Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono SUDARSONO SUDARSONO Sudarsono Sudarsono Sudarsono, dan SUDARSONO, nFn SUDARSONO, nFn SUDARSONO, SUDARSONO Sudirman Yahya Suhartanto , M. Rahmad Suhartanto, Muhammad Rahmad Sukma, Dewi Sumaraow, S. M. Sumaraw, S M Surjono Hadi Sutjahjo Suryanah Suryanah Suryaningsih, E. Suryo Wiyono Susiyanti . Suskandari Kartikaningrum Syaiful Anwar Syarifah Iis Aisyah TRI JOKO SANTOSO Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Tri Wiji Nurani Warid Warid Willy Bayuardi Suwarno Winarto, dan Budi Wulandari, Dyah Retno Wulandari, Dyah Retno Yopi Kurniawan