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Hypothyroidism and stunting around the Merapi Volcano Pramudji Hastuti; Ahmad Hamim Sadewa; Prasetyastuti -; Sunarti -; Arta Farmawati; Ngadikun -; Dianandha Septiana Rubi; Abrory Agus Cahya Pramana
Journal of Community Empowerment for Health Vol 4, No 2 (2021)
Publisher : Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jcoemph.61025

Abstract

Cangkringan is a mountainous area with the possibility of low iodine sources in the soil. Additionally, Cangkringan area is the area nearest to where eruptions of Merapi mountain have occurred, which further could reduce iodine levels in the soil. This study examined the incidence of hypothyroidism due to iodine deficiency and potential links to stunting events on the slopes of Merapi mountain. By using ELISA methods, a total of 97 mothers were tested to detect the triiodothyronine (T3) and thyroid-stimulating hormone (TSH) levels to determine the frequency of hypothyroidism, while 97 children were assessed to check the stunting rates by measuring height compared to age. We found hypothyroidism was 3 (3.1%) out of 97 mothers examined and 30 (30.9%) out of 97 children examined were stunted (18.5% short stature and 12.4% very short stature). There was a significant difference between the mothers’ TSH levels among very short stature and normal stature. We concluded that hypothyroidism in mothers is correlated with stunting children in Cangkringan, Sleman sub-district. Further research is needed to determine the frequency of hypothyroidism and stunting in other slopes of Merapi mountain, where the low-iodine soil composition is potentially related to hypothyroidism and stunting incidence. Thus, further treatment is needed by local health staff and governments to address the negative effects of hypothyroidism and stunting.
POLIMORFISME GEN TRANSFERIN P570S TERHADAP KADAR HEMOGLOBIN (HB) PADA REMAJA PUTRI PONDOK PESANTREN DI YOGYAKARTA Siti Wahyuningsih; Sri Wahyuni; Arta Farmawati; Ahmad Hamim Sadewa; Zainal Arifin Nang Agus
Medika Respati : Jurnal Ilmiah Kesehatan Vol 16, No 3 (2021)
Publisher : Universitas Respati Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (875.228 KB) | DOI: 10.35842/mr.v16i3.545

Abstract

Transferrin P570s. Gene Polymorphism Against Hemoglobin (Hb) Levels in Adolescent Girls Islamic Boarding Schools In YogyakartaBackground: Anemia in adolescent girls is one of the nutrients problem. Transferin P570S gene polymorphism has an effect on glycosylated transferin so it can accelerate the degradation transferin. This can cause a decrease in transferrin function and interfere Fe homeostasis. The lack of Fe intake is one cause of the anemia. Objectives: To investigate correlation P570S transferrin gene polymorphisms with iron status as risk factor for iron deficiency anemia in adolescent girls. Methods: Desain of this study was case control. Eighty one adolescent girls living in the boarding school were included in this study. Blood samples used for biochemical examination of the blood and to detect the presence of P570S transferin polymorphism. Genotype frequencies between groups were analyzed using Chi square test and calculated the Odds Ratio (OR). Comparison of the mean rate of hemoglobin between genotype groups were analyzed statistically using One-way ANOVA test. Results: Transferin P570S polymorphism frequency in anemic girls with the CC was 68.4%, CT was 26.3%, TT was 5.3%, C allele frequency= 81.6% and T allele= 18.4%. The odds ratio (OR) P570S transferrin gene polymorphisms for polymorphic genotypes of 0.783 and for the T allele is 0.991. On the subject of anemia with polymorphic genotypes have hemoglobin levels 11.45 while wild type 11.6 (p= 0.000). On the subject of IDA with polymophic genotypes have hemoglobin levels 11.45 while wild type genotype 11.6 (p= 0.190). Conclusion: Genotype frequencies P570S transferring gene polymorphism in adolescent girls at the boarding school who suffer anemia of 31.6% and 35.7% of IDA. The mean hemoglobin levels in subjects with polymorphic genotype lower than the wild type.
Pengaruh Media Terkondisi Sel Punca Mesensimal (MT-SPM) terhadap Histopatologi Pankreas Tikus Model DM Tipe 2 Stefani Santi Widhiastuti; Bernadia Branitamahisi; Nor Sri Inayati; Ida Ayu Preharsini Kusuma; Demas Bayu Handika; Ahmad Hamim Sadewa; Sofia Mubarika Haryana; Abdurahman Laqif
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 3, No 3 (2018): October 2018
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v3i3.1900

Abstract

Diabetes mellitus tipe 2 merupakan jenis diabetes yang paling banyak terjadi. Berbagai macam terapi telah digunakan untuk menangani DM (Diabetes Mellitus) tipe 2, namun masih terdapat keterbatasan. Penelitian ini dilakukan untuk mengetahui pengaruh MT-SPM terhadap histopatologi pankreas dan jumlah sel normal Langerhans pada tikus model diabetes melitus tipe 2. Penelitian ini merupakan penelitian eksperimental murni dengan desain Posttest Control Group. Sebanyak 27 ekor tikus Sprague Dawley jantan dibagi menjadi 3 kelompok: kontrol normal (K(-)) yaitu 9 tikus sehat, kontrol diabetes (K(+)) yaitu 9 tikus yang diinduksi DM tipe 2 dengan 60 mg/kg BB STZ (i.p) dan 120 mg/kg BB NA (i.p), dan kelompok perlakuan (P) yaitu 9 tikus yang diinduksi DM tipe 2 dan diberi perlakuan dengan 0,1 ml/200 g BB (i.p) setiap 3 hari selama 10 kali. Pada hari ke-30 setelah perlakuan, tikus dikorbankan dan diambil jaringan pankreasnya untuk diuji histopatologi dengan pewarnaan Hematoksilin-Eosin. Hasil tersebut dianalisis dengan program Image J. Hasil penelitian menunjukkan terjadi perbaikan pada profil histopatologi pankreas dan terdapat peningkatan jumlah sel normal di islet Langerhans pada kelompok perlakuan yang diberi MT-SPM dibandingkan kelompok kontrol positif.
GSTM1 Deletion Compensated in mRNA Expression and 4T1 Viability After Editing Using CRISPR/Cas9 Single and Double gRNA Emilia Vivi Arsita; Dwi Aris Agung Nugrahaningsih; Ahmad Hamim Sadewa
The Indonesian Biomedical Journal Vol 15, No 2 (2023)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v15i2.2131

Abstract

BACKGROUND: Glutathione S-transferase Mu-1 (GSTM1) is known to undergo polymorphism and plays role in drug metabolism including Paclitaxel (PTX), the first-line chemotherapy for breast cancer. However, the effect of GSTM1 polymorphism against chemotherapy in breast cancer is limited and unexplored. This study was conducted to explore the effects of single and double guide (gRNA) on the GSTM1 knocked out (KO) and its effect on the response of PTX in the 4T1 cell line.METHODS: The preparatory stage was done by culturing and electroporating 4T1 cells using Ribonucleoprotein of clustered regularly interspaced short palindromic repeats (CRISPR)/Caspase 9 (Cas9). KO validation was examined by quantitative reverse transcription polymerase chain reaction (qRT-PCR), Sanger sequencing, and ICE analysis. The 4T1 viability was examined by MTT Assay.RESULTS: The number of base pairs of GSTM1 after being engineered by single or double gRNA was 86 bases. The DNA quantity of GSTM1 engineered by gRNA was more than using double gRNAs. The mRNA expression of GSTM1 engineered by single gRNA was lower than using double gRNAs. IC50 values of PTX between wildtype and KO were not significantly different, in the range of 30 µM.CONCLUSION: The base-pair length of GSTM1 exon 4 that is knocked out with single and double gRNA have the same number of base pairs. The quantity of GSTM1 DNA and mRNA expression are contrary between single gRNA and double gRNA, and IC50 PTX values in the 4T1 cell line of the control group with single or double gRNA knocked out do not differ markedly. PTX efficiency as chemotherapy is not disturbed in the GSTM1 deletion genetic profile.KEYWORDS: GSTM1, gRNA, Paclitaxel, CRISPR, breast cancer
EFEK POLIMORFISME GENA NITRIT OKSIDA SINTASE3(NOS3) TERHADAP KADAR NITRIT OKSIDA DAN TEKANAN DARAH PADA INDIVIDU TERPAPAR PLUMBUM (The Effect of Polymorphisms Gene Nitric Oxide Synthase3 (NOS3) to Nitric Oxide Level and Blood Pressure on Lead-exposed Men) Hernayanti Hernayanti; Sukarti Moeljopawiro; Ahmad Hamim Sadewa; Bambang Hariono; Subagus Wahyuono
Jurnal Manusia dan Lingkungan Vol 19, No 2 (2012): Juli
Publisher : Pusat Studi Lingkungan Hidup Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jml.18532

Abstract

ABSTRAKPenelitian ini bertujuan untuk mengidentifikasi efek polimorfisme gena nitrit oksida sintase3 terhadap kadar nitrit oks ida (NO) dan tekanan darah pada individu terpapar Plumbum. Metode penelitian menggunakan metode survai dengan rancangan kasus kontrol. Subjek kasus terdiri dari 30 orang pekerja bengkel mobil dan 30 orang subjek kontrol berasal dari pedesaan yang mewakili area yang tidak terpolusi Pb.Genotip individu ditentukan dengan metode PCR~RFLP. Parameter yang diukur adalah kadar NO, tekanan darah sistolik dan diastolik serta kadar Pb. Data dianalisis menggunakan uji t independent. Hasil penelitian menunjukkan bahwa 40% dari subjek kasus, terdeteksi sebagai individu pembawa polimorfisme gena NOS3 dengan genotip GA, sedangkan 60% dari subjek kasus dan subjek kontrol terdeteksi sebagai individu nonpolimorfisme gena NOS3 dengan genotip GG. Hasil uji t menunjukkan untuk parameter NO, tekanan sistolik, diastole serta Pb menunjukkan perbedaan yang sangat nyata an tara individu pembawa polimorfisme gena NOS3 dengan individu nonpolimorfisme. Kadar NO individu pembawa polimorfisme NOS3 lebih rendah dibandingkan individu nonpolimorfism. Sebaliknya kadar Pb, tekanan sistolik dan diastole individu pembawa polimorfisme gena NOS3 lebih tinggi dibandingkan individu nonpolimorfisme. Kesimpulan yang diperoleh adalah adanya polimorfisme gena NOS3 dan paparan Pb menyebabkan ketersediaan NO makin rendah dan meningkatkan kadar Pb, tekanan sistolik dan diastolik. Individu terpapar Pb pembawa polimorfisme gena NOS3 beresiko mengalami penyakit hipertensi yang lebih parah dibandingkan individu nonpolimorfisme terpapar Pb.ABSTRACTThe aim of  these research were to identify  the effect of polymorphisms gene NOS3 to nitric oxide level and blood pressure on lead-exposed men. The research used survey method and case control design. These cases of subject were 30 autorepair workers and 30 subject control from village as non polluted area.  The genotype of individu  investigated  by  Polimerase Chain Reaction (PCR)- Restriction Fragment Length Polymorphisms (RFLP). The main parameters were nitric oxide (NO), blood pressure systole, dyastole and blood lead level. The data were analyzed by t test independent. These result showed that 40 % of cases subject  were detected as  individual polymorphism of gene NOS3 with GA alel. In contrast 60 %  of cases subject and control subject were detected as non individual polymorphism with GG alel. Result t test for three parameters i.e. NO, systole, diastole blood pressure and lead showed highly significant difference (p=0) between individual polymorphism and non polymorphism.  These research can be concluded that polymorphism of gene NOS3 on lead-exposed men influence bioability of  NO, systole and diastole blood pressure. Blood lead level, systole and diastole of individual polymorphism  are  higher than individual polymorphism. The risk of hypertension are more seriously on polymorphisms lead-exposure worker than non polymorphisms.
Pengenalan Nutrigenomik Pada Guru SMA Biologi dan Kimia di Kota Yogyakarta Melalui Program Kemitraan Masyarakat (PKM) Arta Farmawati; Sunarti Sunarti; Dianandha Septiana Rubi; Ahmad Hamim Sadewa; Prasetyastuti Prasetyastuti; Ngadikun Ngadikun
SWARNA: Jurnal Pengabdian Kepada Masyarakat Vol. 3 No. 2 (2024): SWARNA: Jurnal Pengabdian Kepada Masyarakat, Februari 2024
Publisher : LPPM Sekolah Tinggi Ilmu Ekonomi 45 Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55681/swarna.v3i2.1177

Abstract

Perkembangan ilmu pengetahuan dan teknologi berdampak pada pengembangan ilmu nutrisi berbasis molekuler diantaranya adalah nutrigenomik. Nutrigenomik merupakan ilmu yang mempelajari bagaimana nutrisi mempengaruhi ekspresi gen. Gen membawa deoxyribose nucleic acid (DNA) yang berisi informasi genetik yang menentukan sintesis protein, dimana protein tersebut berperan dalam berbagai proses biologis, sehingga sangat mempengaruhi kesehatan. Proses biologis merupakan proses reaksi kimia dalam tubuh yang sudah dipelajari sejak sekolah menengah pertama (SMP) oleh karena itu, pengenalan ilmu nutrigenomik pada guru SMA diharapkan dapat meningkatkan pengetahuan terhadap ilmu nutrigenomik pada guru, sehingga dapat meningkatkan kualitas siswa maupun guru sendiri. Upaya ini kami wujudkan dalam bentuk pengabdian masyarakat yang bekerja sama dengan Musyawarah Guru Mata Pelajaran (MGMP) Biologi dan Kimia Kota Yogyakarta. Kegiatan pelatihan yang diikuti 18 guru dilaksanakan dengan beberapa metode pembelajaran seperti ceramah, praktik di laboratorium, tutorial, dan training of trainer (ToT). Berdasarkan hasil evaluasi, program kemitraan masyarakat (PKM) dapat meningkatkan pengetahuan guru-guru mengenai nutrigenomik secara signifikan (43,33 ± 10,01 vs 66,44 ± 13,66) (P<0,05). Hasil ToT menunjukkan tingkat kepercayaan diri guru-guru dalam menyampaikan informasi terkait dengan nutrigenomik menjadi tinggi. Dengan demikian, program ini dapat mengenalkan sekaligus meningkatkan pengetahuan dan rasa percaya diri guru sebagai kader nutrigenomik untuk menyebarluaskan ilmu nutrigenomik kepada siswa maupun masyarakat.
The Genetic Polymorphisms of CYP3A4*1G and CYP3A5*3 in Javanese Indonesian Population: The Genetic Polymorphisms of CYP3A4*1G and CYP3A5*3 in Javanese Indonesian Atmaja, Sarah; Ellsya Angeline Rawar; Ani Kristiyani; Dwi Aris Agung Nugrahaningsih; Ahmad Hamim Sadewa; Christine Patramurti
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.06

Abstract

Polymorphisms of CYP3A4*1G and CYP3A5*3 affect the pharmacokinetic profile of various drugs, e.g., fentanyl, tacrolimus, diltiazem, simvastatin. Tetra-primer amplification refractory mutation system-polymerase chain (ARMS-PCR) is a simple and economical method for SNP determination. The polymorphisms in the CYP3A4*1G and CYP3A5*3 genes have not yet been examined using this method in Javanese Indonesian. Our aim was to determine the frequency of polymorphisms in the CYP3A4*1G and CYP3A5*3 genes in Indonesian Javanese using the ARMS-PCR method. Eighty-six patients at the Kalasan Community Health Centre in Yogyakarta, Indonesia, were chosen based on the inclusion criteria, which is Javanese ancestry. They gave their informed consent to blood collection by completing a form. Genetic variants were detected using Tetra-primer amplification refractory mutation system-polymerase chain (ARMS-PCR). The chi-square test was used to determine genotype deviations from Hardy-Weinberg equilibrium, with a significant threshold of 0.05. For homozygous wild types, CYP3A4 *1/*1 dominated overall among study participants (73.35%), whereas for CYP3A5*3/*3, homozygous mutants were more prevalent (83.72%). Hardy-Weinberg equilibrium is consistent with genotype frequencies (p > 0.005). One participant carried a homozygous mutation for both CYP3A4*1G and CYP3A5*3, while the other 49 subjects were heterozygous for CYP3A4*1G and homozygous mutant for CYP3A5*3, which is the highest number of SNP combinations. The findings of the current investigation demonstrate that the population has the highest proportion of homozygous CYP3A4*1G wild-types (CYP3A4*1/*1) and homozygous mutants for CYP3A5*3 (CYP3A5*3/*3)
Polimorfisme Gen Ferroportin (FPN1) Q248H dan Karakteristik Sosial Ekonomi Ibu Hamil dengan Anemia di Surakarta Istiqomah, Nor; Dewi, Vitria Sari; Farmawati, Arta; Sadewa, Ahmad Hamim; Soesilo, Yuliana Heri; Damayanti, Kusumadewi Eka; Indarto, Dono
Pena Medika Jurnal Kesehatan Vol 4, No 1 (2013): PENA MEDIKA JURNAL KESEHATAN
Publisher : Universitas Pekalongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31941/pmjk.v4i1.284

Abstract

Background: Iron deficiency anemia in pregnancy remains an important health problem in Indonesia. Ferroportin (FPN1) is one of important gene in iron metabolism encodes ferroportin for iron absorption, release, and recycle inside the body. The polymorphism of FPN1 Q248H alters negative charge of the amino acid sequence positively, hence influenced its ubiquitination and degradation. Beside of that, the high prevalence of anemia not only caused by genetics, nutrition, physiology of each pregant women, but also demographic factor and sosio-economic burden of family. This research will study the polymorphism of ferroportin Q248H and demographic character in pregnant women in Surakarta.Methods: The study using descriptive analytic case-control approach. The data used were from questionaire and blood sampling whose counted for screening anemia in private laboratorium. Pregnant women who had intention and had 10-25 week in pregnant were selected to be research subject.  FPN1 Q248H polymorphism were determined using PCR-RFLP method. Analysis Chi-square dan Independent t-test used to conclude the relationship between each variable to anemic state of pregnant women in Surakarta. A p<0.05 was considered as significant. The study using decriptive analytic design method. Results: Prevalence of anemia in Surakarta is 25.7% with 80.7% have mild anemia. There was no varian in FPN1 Q248H. Majority of pregnant women in group of anaemia have demographic character like 20-25 old, multigravid, last education in High School, housewife, their income range from Rp 500.000,00 to Rp 1.000.000,00, and consume Fe tablet. The bivariat analysis show no statistical significancy of parity, maternal age, education, income, and comsumption of iron tablet to state of anaemia (p>0.05).Conclusions: The FPN1 Q248H polymorphism was not a risk factor for iron deficiency anemia in pregnant women, likewise socio-economic factors had no significant role to incidence of anemia in Surakarta. Keywords: Anemia in pregnancy, Ferroportin (FPN1) Q248H polymorphism, Socio-Economic
CEA and Cyfra 21-1 linked to serial miRNA expressions of advanced-stage non-small cell lung cancer in Indonesia Hanafi, Arif Riswahyudi; Jayusman, Achmad Mulawarman; Imelda, Priscillia; Alfasunu, Serafim; Sadewa, Ahmad Hamim; Pramono, Dibyo; Heriyanto, Didik Setyo; Haryana, Sofia Mubarika; Kresno, Siti Boedina
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 55, No 4 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19106/JMedSci005504202303

Abstract

Globally, lung cancer is one of the cancers leading to dead, dominated by non-small cell lung cancer (NSCLC). In a previous study has shown those serial miRNA expressions (miR-148, miR-34, miR-222, and miR-155) had prognostic value in advanced-stage NSCLC patients. Meanwhile, CEA and Cyfra 21-1, pulmonary tumor markers, are sometimes considered in the Department of Pulmonology, Dharmais Cancer Hospital, Jakarta, although they are not used in routine clinics for prognostication. Both miRNA and CEA-Cyfra 21-1 are valuable biomarkers in NSCLC. This study aimed to evaluate their correlation between CEA and/or Cyfra 21-1 with miRNA expressions in NSCLC patients. It was a cohort retrospective study using data from the previous study. The correlation between variables was analyzed by Spearman-rho. A positive correlation was observed between CEA and Cyfra 21-1 with miR-148, miR-222, and miR-155 [(CEA: p=0.00369, r=0.522; p=0.00242, r=0.542; p 0.00106, r=0.576) (Cyfra: 21-1= p 0.01252, r=0.378; p=0.00035, r=0.519; p=0.01532, r=0.368)]. In conclusion, CEA and Cyfra 21-1 correlate with miR-148, miR-222, and miR-155 expressions in advanced-stage NSCLC.
Pengenalan Nutrigenomik Pada Guru SMA Biologi dan Kimia di Kota Yogyakarta Melalui Program Kemitraan Masyarakat (PKM) Farmawati, Arta; Sunarti, Sunarti; Rubi, Dianandha Septiana; Sadewa, Ahmad Hamim; Prasetyastuti, Prasetyastuti; Ngadikun, Ngadikun
SWARNA: Jurnal Pengabdian Kepada Masyarakat Vol. 3 No. 2 (2024): SWARNA: Jurnal Pengabdian Kepada Masyarakat, Februari 2024
Publisher : LPPM Sekolah Tinggi Ilmu Ekonomi 45 Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55681/swarna.v3i2.1177

Abstract

Perkembangan ilmu pengetahuan dan teknologi berdampak pada pengembangan ilmu nutrisi berbasis molekuler diantaranya adalah nutrigenomik. Nutrigenomik merupakan ilmu yang mempelajari bagaimana nutrisi mempengaruhi ekspresi gen. Gen membawa deoxyribose nucleic acid (DNA) yang berisi informasi genetik yang menentukan sintesis protein, dimana protein tersebut berperan dalam berbagai proses biologis, sehingga sangat mempengaruhi kesehatan. Proses biologis merupakan proses reaksi kimia dalam tubuh yang sudah dipelajari sejak sekolah menengah pertama (SMP) oleh karena itu, pengenalan ilmu nutrigenomik pada guru SMA diharapkan dapat meningkatkan pengetahuan terhadap ilmu nutrigenomik pada guru, sehingga dapat meningkatkan kualitas siswa maupun guru sendiri. Upaya ini kami wujudkan dalam bentuk pengabdian masyarakat yang bekerja sama dengan Musyawarah Guru Mata Pelajaran (MGMP) Biologi dan Kimia Kota Yogyakarta. Kegiatan pelatihan yang diikuti 18 guru dilaksanakan dengan beberapa metode pembelajaran seperti ceramah, praktik di laboratorium, tutorial, dan training of trainer (ToT). Berdasarkan hasil evaluasi, program kemitraan masyarakat (PKM) dapat meningkatkan pengetahuan guru-guru mengenai nutrigenomik secara signifikan (43,33 ± 10,01 vs 66,44 ± 13,66) (P<0,05). Hasil ToT menunjukkan tingkat kepercayaan diri guru-guru dalam menyampaikan informasi terkait dengan nutrigenomik menjadi tinggi. Dengan demikian, program ini dapat mengenalkan sekaligus meningkatkan pengetahuan dan rasa percaya diri guru sebagai kader nutrigenomik untuk menyebarluaskan ilmu nutrigenomik kepada siswa maupun masyarakat.
Co-Authors . Harapan . HERNAYANTI . Syahrul Abdul Salam M Sofro, Abdul Salam Abdul Salam M. Sofro Abdul Salam Mudzakir Sofro Abdul Salam Mudzakir Sofro, Abdul Salam Mudzakir Abdurahman Laqif Abdurahman Laqif Abdus Samik Wahab Abrory Agus Cahya Pramana Ahmad Husain Asdie Ahmad Husain Asdie Ahmad Husain Asdie Ajeng Viska Icanervilia Akhmad Kharis Nugroho Alfasunu, Serafim Anggelia Puspasari Anggoro Budi Hartopo Ani Kristiyani Arif Faisal Arif Faisal Arta Farmawati Arta Farmawati Atmaja, Sarah Awal Prasetyo Bai Apris Bambang Hariono Bambang Hariono Bambang Hariono Bansai Immanuel Bernadia Branitamahisi Bernadia Branitamahisi Budi Yuli Setianto Cahyono Hadi Cahyono Hadi Christine Patramurti Citra Maharani Danarsih, Dwi Eni Darojatun Ida Demas Bayu Handika Dessy Rakhmawati Emril Dewajani Purnomosari Dian Caturini Sulistyonigrum Dian Lestari, Nova Dianandha Septiana Rubi Dibyo Pramono Didik Setyo Heriyanto Djaswadi Dasuki Djaswadi Dasuki Djaswadi Dasuki Dono Indarto Dono Indarto DONO INDARTO Dwi Aris Agung Nugrahaningsih Dyah Wulan Anggrahini Ellsya Angeline Rawar Emilia Vivi Arsita Emy Huriyati Endang Mutiawati Rahayuningsih* Faisal Rahman Fransisca Shinta Maharini Hanafi, Arif Riswahyudi Hari Kusnanto Hasan Sjahrir Hemi Sinorita Hendi Wicaksono Hernayanti , Hernayanti Hernayanti Hernayanti HERNAYANTI HERNAYANTI Hexa Apriliana Hidayah I Gusti Ayu Nyoman Danuyanti I NYOMAN MANTIK ASTAWA Ida Ayu Preharsini Ida Ayu Preharsini Kusuma Ika Rahayu Ika Setyawati Ikhsan, M. Robikhul Imelda, Priscillia Indwiani Astuti Indwiani Astuti Indwiani Astuti Inna Narayani Iskandar Zakaria Jayusman, Achmad Mulawarman Jenny Hidayat Jontari Hutagalung Kik Hao Samuel Kris Herawan Timotius Kusumadewi Eka Damayanti Kusumadewi Eka Damayanti Kusumadewi Eka Damayanti Lina Choridah Linawati Hananta, Linawati Lucia Krisdinarti Lukman Hakim M. Robikhul Ikhsan Maliyah Madiyan Maria Dara Novi Handayani, Maria Dara Novi Marsetyawan HNE Soesatyo Mega Tyas Prihatin Mohammad Robikhul Ikhsan Mus, Rosdiana Mustofa Mustofa Mustofa Mustofa Ngadikun - Ngadikun Ngadikun Nor Istiqomah Nor Istiqomah Nor Istiqomah Nor Sri Inayati Nor Sri Inayati Novijanti Rintis Nurtjahjo Dwi Sasongko P. Purwono Pinda Hutajulu Pinda Hutajulu, Pinda Pramudji Hastuti Pramudji Hastuti Pramudji Hastuti Pramudji Hastuti Pramudji Hastuti Pramudji Hastuti Pramudji Hastuti Pramudji Hastuti, Pramudji Prasetyastuti Prasetyastuti Prasetyastuti Prasetyastuti Prasetyastuti Prasetyastuti, . Rasmaya Niruri Ratih Feraritra Danu Atmaja Rina Pratiwi Pudja I. A Rina Susilowati RIZKI FAJAR UTAMI Rochadi Rochadi Rochadi Rochadi, Rochadi S. Supargiyono Sabirin, Rahmaningsih Mara Sarah Safira Umarghanies Saryono Saryono Satyagraha Ari Winasti Seto Priyambodo Setyo Purwono Siti Boedina Kresno Siti Wahyuningsih Sofia Mubarika Haryana Sri Sutarni Sri Wahyuni Subagus Wahyuono Subagus Wahyuono Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sunarti Sunarti Sunarti Sunarti Sunarti Sunarti Sunarti Sunarti, Sunarti Sunarto Ang Tasmini - Tasmini Tasmini Teguh Aryandono Titiek Suhardi Haripurnomo Kushadiwijaya Hidayati Totok Utoro Totok Utoro Triwibowo A. Garjito Umarghanies, Sarah Safira Viren Ramadhan Vitarani Dwi Ananda Ningrum Vitria Sari Dewi Vitria Sari Dewi Waode Astria Sahrani Wasilah Rochmah wayan T Artama wayan T Artama Wayan Tunas Artama Widhiastuti, Stefani Santi Wiryatun Lestariana Wiryatun Lestariana, Wiryatun Wiwik Handayani Yuliana Heri Soesilo Yuliana Heri Soesilo Yuliana Heri Soesilo Yuliani, Fara Silvia Yunilistiaingsih Yunilistiaingsih Yunilistiaingsih, Yunilistiaingsih Yuningtyaswari Yuningtyaswari Zainal Arifin Nang Agus Zullies Ikawati