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All Journal Jurnal Sain Veteriner Jurnal Veteriner Media Kedokteran Hewan Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Aceh Journal of Animal Science Jurnal Medik Veteriner Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Journal of Applied Veterinary Science and Technology journal of Basic Medical Veterinary

Endang Suprihati

Division Of Veterinary Parasitology, Faculty Of Veterinary Medicine, Universitas Airlangga

Author-ID : 393918

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Detection of Blood Protozoa Infecting Broiler Chicken Farms in Tanjung Gunung Village, District Jombang Marchelia Arifiandani; Endang Suprihati; Wiwik Misaco Yuniarti; Nunuk Dyah Retno Lastuti; Poedji Hastutiek; Sunaryo Hadi Warsito
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

Broiler chicken farms could be a part of potential source infection Leucocytozoonosis and Plasmodiosis disease due to the death of livestock. Broiler farms in Tanjung Gunung, District Jombang near the fields, water, and trees. This could be increase the population of vectors infection Leucocytozoonosis and Plasmodiosis. The main purpose of this researchis to detect Leucocytozoonosis and Plasmodiosis infections on broiler farms in Tnjung Gunung Village, District Jombang using 50 broiler chickens by Purposive Sampling of 2 bredeers. The result show rhat there are 6 positive samples infected Leucocytozoonosis, while Plasmodiosis infection could not be fond. Need cage sanitation to break life cycles of vector Leucocytozoonosis and Plasmodiosis.

The Anthelmintics Activity of Ethanol Extract of African Leaf (Vernonia amygdalina) on Mortality of Fasciola gigantica in Vitro Dhio Asmaydo; Iwan Sahrial Hamid; Kusnoto Kusnoto; Mohammad Sukmanadi; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The Aims of this study is to know the anthelmintics activity of ethanol extract of african leaf (Vernonia amygdalina) on mortality of Fasciola gigantica in Vitro. Method that used in the research was a completely randomized design. There were five treatments and each treatment was done in four replications. This research used ten Fasciola gigantica in each treatment for all replications. The observation and recording of dead Fasciola gigantica was done at 2, 4, 6, 8, and 10 hours.Â Fasciola gigantica were declared dead if there was no movement when disturbed by anatomy tweezers and when dipped in slightly warm water (500C). The obtained data was analyzed using ANOVA and continued with Duncan Multiple Range Test. The result of this research show that ethanol of extract of African Leaf (Vernonia amygdalina) has activity anthelmintic on mortality of Fasciola gigantica in Vitro. Ethanol extract african leaf (Vernonia amygdalina) with concentration of 1.4% is the optimal concentration of anthelmintics. This higher the concentration of the extract, the higher the property of anthelmintics.

The Anthelmintic Activity of Ethanol Extract of Bitter Leaf (Vernonia amygdalina) Against Ascaridia galli Worm in Vitro Amelia Dwita Safitri; Iwan Sahrial Hamid; Poedji Hastutiek; Setiawan Koesdarto; Rahmi Sugihartuti; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The aims of this study is to know the anthelmintic activity of ethanol extract of bitter leaf (Vernonia amygdalina) against Ascaridia galli worm in Vitro, as well as knowing effective concentration 50 (EC50) and lethal time 50 (LT50). Method that used in the research was completely randomized design. There were five treatments of physiological NaCl solution (K-), piperazine sitrate (K+), etanol extract of bitter leaf 0,35% (P1), etanol extract of bitter leaf 1,4% (P2), etanol extract of bitter leaf 4,2% (P3), and each treatment was done in four replications. This research used ten Ascaridia galli in each treatment for all replications. The observation and recording of dead Ascaridia galli was done at 2, 4, 6, 8, 10 and 12 hours. Ascaridia galli were declared dead if there was no movement when disturbed by anatomy tweezer and when dipped in slightly warm water (50°C). The obtained data was analyzed using ANAVA and continued with Duncan Multiple Range Test. The result of this research show that etanol extract of bitter leaf (Vernonia amygdalina) has anthelmintic effects against Ascaridia galli worm in Vitro. In the extract with 4,2% concentration, there is anthelmintic property that almost the same as Piperazine sitrate 10 mg/ml. the higher the concentration of extract, the higher the property of anthelmintic. In probit analysis show that EC50 achieved by concentration 2.093% with the low concentration of .002% and the highest concentration of 3.632%. LT50 of ethanol extract of bitter leaf was 0.35% at 10.323 hours, 1.4% at 9.800 hours, 4.2% at 7.864 hours and Piperazine sitrate 10 mg/ml at 9.013 hours.

The Effectiveness of Ethanol Extract of Red Betel Leaf (Piper crocatum) Againts Mortality of Boophilus microplus Larvae in Vitro Meta Aprilia; Poedji Hastutiek; Rochmah Kurnijasanti; Lucia Tri Suwanti; Mohammad Sukmanadi; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The aims of this study is to know the effectiveness of ethanol extract of red betel leaf (Piper crocatum) against mortality of Boophilus microplus Larvae in Vitro, as well as knowing lethal concentration 50 (LC50) and lethal concentration 90 (LC90). Method that used in the research was completely randomized design. There were five treatments and each treatment was done in five replications. This research used ten Boophilus microplus larvae in each treatment for all replication. The observation and recording of mortality was done at 1, 2, 3, 4, and 5 hours. Boophilus microplus larvae were declared dead if there was no movement. The obtained data was analyzed using ANOVA factorial. The result of this research show that ethanol extract of red betel leaf (Piper crocatum) has effectiveness against mortality of Boophlius microplus larvae in Vitro. In the extract 10% concentration, there is effectiveness that almost the same as Noticks®. The higher concentration of the extract, the higher the effectiveness against mortality lavae.In probit analysis show that LC50 achieved by concentration 5,4% or 2.700mg/50ml and LC90 achieved by concentration 11,3% or 5.650mg/50ml.

The Prevalence and Infection’s Degree of Gastrointestinal Worm of Local Chicken (Gallus domesticus) in Kramat Village, District of Bangkalan, Madura, East Java Indonesia Ellza Agatha Damayanti; Poedji Hastutiek; Agnes Theresia Soelih Estoepangestie; Nunuk Dyah Retno Lastuti; Kusnoto Kusnoto; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The study was aimed to know the prevalence and level of gastrointestinal worm investation of local chicken (Gallus domesticus) in Kramat Village, district of Bangkalan, Madura East Java province. The samples used in the study were 100 faeces of kampung chicken. using sedimentation and floating method. Faeces samples were tested, than followed by microscopically test to identify the worm is species. The result showed that in 38 out of 100 samples could be indentified some worm species as followed : Capillaria sp.14 (36,8%), Railletina 7 (18,4%), Hymenolepis 16 (42,1%) and Heterakis gallinarum 1 (2,6%). Using Mann Whitney U-test could be differentiated the level of the infection with an average of 45.53 in cock, while in hen with an average of 55.47. There was no significancy (p>0,05) on the level of worm gastrointestinal infection of different sex of kampung chicken.

Prevalence of Gastrointestinal Parasite on Cats in Shelter East Surabaya Akbar Wijaya Putra Purnama; Lucia Tri Suwanti; Hani Plumeriastuti; Endang Suprihati; Kusnoto Kusnoto; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this study was to determine the prevalence of gastrointesatinal parasites in cats at Surabaya shelter. Eighty two samples of cat feces used in this study were taken from Griya satwa shelter and Kiro cat shelter. Feces samples were examined using the native, sedimentation and floating methods. Feces samples gastrointestinal parasite were identified by using a microscope with 400x and 1000x magnifications. Faces samples that are diagnosed positively if there is at least one of the three methods. Feces samples has observed, obtained prevalence rate of 43.9% or 36 positive samples from eighty two samples. The results of chi square analysis showed that p > 0.05, which showed that the parasites taken in the two shelters had clear differences in the prevalence of the gastrointestinal tract parasites. The parasites observed were Isospora sp., Ancylostoma sp., Toxocara cati and Toxocara leonina.

Identification of Gastrointestinal Endoparasite in Bawean Deer (Axis kuhlii) and Spotted Deer (Axis axis) at Bratang Flora Park – Surabaya Hartono Hartono; Endang Suprihati; Erma Safitri; Nunuk Dyah Retno Lastuti; Mufasirin Mufasirin; Kusnoto Kusnoto
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this study is to identify the endoparasites of Spotted Bawean Deer (Axis kuhlii) and Deer (Axis axis ) at Surabaya Flora Park. The research was conducted in December 2018 - January 2019 used total 60 specimen of sample faeces, consist of 30 sample of bawean deers faeces and 30 sample of spoted deers faeces, the samples analyzed at the Department of Parasitology Veterinary, Faculty of Veterinary Medicine, Universitas Airlangga. The results of the study found positive endoparasites on examination, samples of Bawean deer faeces (Axis kuhlii) and Spotted deer faeces(Axis axis) in Baratang Flora Park Surabaya found that 26 positive samples from 60 samples were obtained. Positive endoparasitic samples are protozoa at the stage of oocyst, namely Eimeria sp. 6 (10%) samples, worm eggs from the Nematoda class, Haemonchus sp. 20 (33.33%) and whereas endoparasites from other stages and classes were not found. The results of the analysis used Chi-Square test and showed no significantly differences degree of gastrointestinal endoparasite infection of Bawean deer (Axis kuhlii) and Spotted deer (Axis axis) (p>0.05).

Cross Reaction of Haemonchus Contortus ANTIGEN WITH ANTI-Fasciola Gigantica Serum by Using Western Blot Technique Firman Hadi Fanani; Kusnoto Kusnoto; Poedji Hastutiek; Setiawan Koesdarto; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this research to know cross reaction of Haemonchus contortus protein with anti-Fasciola gigantica serum by using the Western Blot technique. The result can be used as Haemonchosis serologic diagnostic. This research was conducted using Haemonchus contortus worms obtained from abomasum, especially cow worms and Fasciola gigantica worms obtained from the bile duct and gall bladder. The worms were crushed and added with Phosphate Buffer Saline (PBS) to make homogenates and then centrifuged at 3500 rpm for 15 minutes. Homogenate Fasciola gigantica was immunized to mice to get anti-Fasciola gigantica serum at a dose of 200 Î¼g/mice and booster 3 times with a span of two weeks. Homogenates were carried out using the SDS-PAGE technique to analyze proteins using a brilliant blue dye. Cross reaction of Haemonchus contortus protein with anti-Fasciola gigantica serum byusing Western blot technique and obtained 3 protein bands are 94.9, 52.7, 46.7 kDa.

Prevalensi Ektoparasit pada Kambing Kacang di Kecamatan Prambon Kabupaten Nganjuk Nining Sari Virgandina Vinola; Agus Sunarso; Nenny Harijani; Endang Suprihati; Poedji Hastutiek; Mufasirin Mufasirin
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this research are to identify and prevalence of ectoparasite infestation in bean goats on the sub-district of Prambon, district of Nganjuk. The research was conducted from September until December 2019 with 115 samples of bean goats. The samples were identified in the Entomology and Protozoology Laboratory of Parasitology Department of the Veterinary Faculty of Airlangga University. This research use non-experimental method with survey study. This research found several kind of extoparasites that infestation bean goats, as follows: Bovicola caprae 24.3% the most infestation in dorsal region, Linognatus africanus 31.2% the most infestation in head-neck and dorsal region, Ctenocepallides felis 4.3%. Mix infestation found B. Caprae and L. Africanus 20.8%, B. Caprae and C. Felis 1%, B. Caprae, L. Africanus and C. Felis 1%. This ectoparasite usually found infestatio in head-neck, ventral, dorsal, inguinal, and ectremites. The prevalence of ectoparasites is 82.6% area at sub-district of Prambon, district of Nganjuk. The data analyzed using Chi-square test and Correspondence test. Statistically is no significant association (p>0.05) between the prevalence of ectoparasites that infested bean goats with types of stage and non-stage eclosure. This caused due to environmental conditions are dirty and many other livestock around the cage.

Prevalensi dan Derajat Infeksi Cacing Saluran Pencernaan pada Itik Jawa (Anas javanica) di Dua Daerah Geografis Berbeda Dian Ayu Permatasari; Kusriningrum Rochiman; Tjuk Imam Restiadi; Sri Mumpuni Sosiawati; Endang Suprihati; Mustofa Helmi Effendi
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

The aim of this study was to determined the prevalence and infection disease degree of worm gastrointestinal on duck in two different geographical territories. The duck feces examination samples were using natif method, sedimentation, and floatation. The result of this examination of lowlands showed that 20% (12/60) ducks checked that infected by two worm species of nematoda, wich ia Ascaridia galli (11,7%), Heterakis sp. (8,3%), with the range of infection degree classified as low infection (0-500 EPG), while the examination results of highlands showed that 44% (33/75) ducks checked infected by two worm species of nematoda, which is Ascaridia galli (17,3%), Heterakis sp. (10,7%) and one species of trematoda, which is Echinostoma sp. (6,7%), with the range infection degree classified low infection (0-500 EPG). it can get both single or mix infection. The analysis result has shown that there was differences between lowlands and highlands on the prevalence and infection disease degree of worm gastrointestinal on duck, was highly significant (p<0,05).

Co-Authors Adi Sofyan Ansori, Muhammad Afiyah Rahmadani Agnes Theresia Soelih Estoepangestie Akbar Wijaya Putra Purnama Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Alasrorik, Muhammad Hizbulloh Aldin Akbar Rahmatullah Amara Lintang Pagati Amelia Dwita Safitri Anam Al Arif Anggreani Desi Ramadhani Rahajeng Arif Pratiwi Arimbi Arimbi Ayu, Zerlinda Dyah Azizah Bilqis Nurkarimah Azwani, Nabila Azzahra, Farah Nabila Boedi Setiawan Cindy Ercha Aulia Putri Dadik Rahardjo, Dadik Dadik Raharjo David Mohamad Qadafi Dhio Asmaydo Dian Ayu Permatasari Dian Ayu Permatasari Didik Handijatno Doohan Mahendra DWI PUTRI RAHMAWATI Dyah Ayu Kurniawati Dyah Ayu Kurniawati Ellza Agatha Damayanti Emy Koestanti Sabdoningrum Eny Coolfina Simarmata Erma Safitri Fadila Zikri Amanda Faisal Fikri Fatmawati, Mira Fatmawati Fedik Abdul Rantam Firman Hadi Fanani Fransiska Cicilia Beka Ghifari Lutfi Fauzi Hana Eliyani Hani Plumeriastuti Hartono Hartono Hayuning Nurrodhiya Heni Puspitasari Imam Mustofa Indasari, Elly Nur Ismudiono Ismudiono, Ismudiono Iwan Sahrial Hamid Iwan Sahrial Hamid Jihaan Haajidah Khairullah, Aswin Rafif Kiki Amalia Rama Koesdarto Koesdarto Kuncoro Puguh Santoso Kurniawan, Muhammad 'Ahdi Kurniawati, Dyah Ayu Kusnoto Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Kusriningrum Rochiman Legowo, Djoko Lianny Nangoi Lilik Maslachah Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Marchelia Arifiandani Masdiana C Padaga Maudika Pamela Fiorenza Meta Aprilia Meyreta Doti Alcaterana Mirni Lamid Mochamad Lazuardi Mohammad Sukmanadi Moses, Ikechukwu Benjamin Mufasirin Muhammad Fahmi Abdillah Muhammad Hambal Mustofa Helmi Effendi Nenny Harijani Ni Komang Aprilina Widisuputri Nidom, Chairul Anwar Niluh Selly Frantika Nining Sari Virgandina Vinola Nizar Bachrudin Prihandono Nunuk D.R. Lastuti Nunuk Dyah Retno Lastuti Nunuk Dyah Retno Lastuti Nurin Ardhiani Panjaitan, Cahaya Poedji Hastutiek Praja, Ratih Novita Pratama, Bima Putra Pratama, Ponasari Galuh Pratiwi, Arif Prawasa, Ramadhiniyanti Putri Alif Pudji Srianto Puput, Sesa Rahmi Sugihartuti Rahmi Sugihartuti, Rahmi Ratna Damayanti Retno Wulansari Riwu, Katty Hendriana Priscilia Rizki, Andaru Rochmah Kurnijasanti Romy Muhammad Dary Mufa Sandy, Berliana Dwi Nandita Sarmanu, Sarmanu Setiawan Koesdarto Setiawan Kusdarto Soeharsono Soeharsono Sri Mumpuni Sosiawati Sri Pantja Madyawati, Sri Pantja Sunarso, Agus Supranianondo, Koesnoto Suzanita Utama Tjuk Imam Restiadi Trilas Sardjito Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widi Nugroho Widodo, Oky Setyo Wiwik Misaco Yuniarti Yudhana, Aditya Yulianna Puspitasari Yunus, Muchammad

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