Endang Suprihati
Department Of Veterinary Parasitology, Faculty Of Veterinary Parasitology, Universitas Airlangga, Surabaya

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EFEKTIVITAS PEMBERIAN NANOPARTIKEL EKSTRAK DAUN JUWET (Syzygium cumini) SEBAGAI ADJUVANT TERAPI TERHADAP GAMBARANHISTOPATOLOGI PULMO MENCIT (Mus musculus) YANG DIINFEKSI Plasmodium bhergei Ayu, Zerlinda Dyah; Maslachah, Lilik; Supranianondo, Koesnoto; Suprihati, Endang; Plumeriastuti, Hani; Sugihartuti, Rahmi
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21091

Abstract

The purpose of this study was to learn how to use juwet nanoparticle leaf extract (Syzygium cumini) as an adjunct therapy in pulmonary histopathological mice (Mus musculus) supported by Plasmodium berghei. This study used 30 rats divided into six groups consisting of K0 (disagree without agreement), P1 (fight without agreement), P2 (fight and dispute with chloroquine), P3 (fight and talk with juwet leaf extract), P4 (useful and useful with juwet leaf extract)) and P5 (extracts and combinations given with chloropine and juwet leaf extract nanoparticles) protected by 1x105 in 0.2 ml of Plasmodium berghei. Data were analyzed by Kruskal Wallis using SPSS and continued with the Mann-Whitney test. The results of this study showed that there were significant differences (p <0.05) between P2 and P5, but did not show a significant difference (p> 0.05) with P1, P3 and P4. Based on this study, can be witnessed by juwet nanoparticles (Syzygium Cumini) additional therapeutic leaf extract which is expected to reduce pulmonary damage (hemmoragi, alveolar congestion, edema, hemosiderin) mice (Mus musculus) infected with Plasmodium berghei. 
Nephroprotective Effect of Dayak Onion (Eleutherine palmifolia) Against Monosodium Glutamate-Induced Renal Toxicity in Mice (Mus musculus) Anggreani Desi Ramadhani Rahajeng; Aldin Akbar Rahmatullah; Cindy Ercha Aulia Putri; Rahmi Sugihartuti; Endang Suprihati; Hani Plumeriastuti; Mohammad Sukmanadi; Iwan Sahrial Hamid
Journal of Applied Veterinary Science And Technology Vol. 5 No. 2 (2024): October 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V5.I2.2024.129-134

Abstract

Background: The toxicological impacts of monosodium glutamate (MSG), a commonly used food additive, present a significant public health concern. Excessive MSG consumption will increase radical oxidative species, inducing a stress oxidative condition that ultimately results in kidney damage. Purpose: This study aimed to explore the nephroprotective effects of ethanol extracts from Dayak onion (Eleutherine palmifolia) against MSG-induced renal toxicity in mice (Mus musculus). Method: Twenty-five 11 week-old male mice, weighing approximately 20 g each, were divided into five research groups. Group C- received only CMC-Na 0.5%, Group C+ received 4000 mg/kg BW MSG, while Groups T1, T2, and T3 were administered 4000 mg/kg BW MSG along with Dayak onion extract at doses of 30 mg/kg BW, 60 mg/kg BW, and 120 mg/kg BW respectively. All treatments were carried out orally for 52 days. The mice were euthanized by cervical dislocation, and their kidneys were extracted for the examination of any histopathological changes. The data underwent analysis utilizing the Kruskal-Wallis test, followed by the Mann-Whitney test. Results: The results as indicated by the histopathological evaluation of the mice’s kidneys revealed significant improvements in the histo-architecture of the kidneys. Supplementation of Dayak onion extract in mice induced with MSG decreased the degeneration and necrosis of the tubule epithelium, and it also repaired the glomerular necrosis. Conclusion: Oral administration of Dayak onion extract to mice exposed to monosodium glutamate demonstrated a notable reduction in kidney damage and helped maintain renal health significantly.
Detection of Nematode on Feces of Snail at Malang, East Java Niluh Selly Frantika; Kusnoto; Lucia Tri Suwanti; Imam Mustofa; Endang Suprihati; Hani Plumeriastuti
Journal of Parasite Science Vol. 9 No. 1 (2025): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v9i1.65007

Abstract

Acathina fulica is a terrestrial mollusk that often invades terrestrial vegetation that has tropical and subtropical climates. Acathina fulica has been widely reported in environments as diverse as urban areas, forests, capoeiras, caatings, plantations, decomposing materials, savannahs and vegetable gardens. Soil is source of food and a defense strategy for A. fulica, therefore it has an important role for them. Therefore, A. fulica may be host several species of nematodes. Microscopic examination of snails A. fulica fecal samples in Cemorokandang Village, Malang City using the native method aimed to detect Rhabditis spp. The results showed that 14 (12.5%) out of 112 samples contained Rhabditis spp. The genus rhabditis belongs to the Rhabditidae family and is one of the free-living nematodes. This species has long been known and of concern in the veterinary world. The genus rhabditis has been reported to have infected humans and have been isolated from urine samples, vaginal swab, and human stool. The research was conducted in the dry season with low rainfall. Nematode of the genus Rhabditis spp. can be naturally found in humid places and decaying organic matter.
Morphometric and Molecular Identification of Eimeria Bovis and Eimeria Zuernii on Beef Cattle in Lamongan, East Java, Indonesia Kurniawan, Muhammad 'Ahdi; Suwanti, Lucia Tri; Mufasirin, Mufasirin; Suprihati, Endang; Hastutiek, Poedji; Kusnoto, Kusnoto; Ansori, Arif Nur Muhammad; Puspitasari, Yulianna; Khairullah, Aswin Rafif; Moses, Ikechukwu Benjamin; Pratama, Bima Putra; Riwu, Katty Hendriana Priscilia
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.153-166

Abstract

Lamongan Regency, located in East Java, Indonesia, is a significant center for beef cattle production. Despite its prominence, studies on the identification and differentiation of Eimeria spp. parasites in this region are notably lacking. This research aims to address this gap by evaluating the prevalence of Eimeria spp. and identifying two pathogenic species: Eimeria bovis and Eimeria zuernii. The study involved the collection of 250 fecal samples from beef cattle raised on smallholder farms across Lamongan. Sampling was conducted during the rainy season to optimize the detection of Eimeria infections. Using the sugar flotation method, Eimeria oocysts were isolated from 5–10 g of fecal matter per sample. Molecular identification employed polymerase chain reaction (PCR) assays targeting ribosomal RNA’s internal transcribed spacer 1 (ITS-1) region to detect the pathogenic species. Fecal examination using the Whitlock test revealed a prevalence of Eimeria spp. at 44.45% (111/250). PCR analysis further identified E. bovis with a 238 bp amplicon in Solokuro District and E. zuernii with a 344 bp amplicon in Tikung District, highlighting the presence of these two pathogenic species in distinct geographic areas. The findings underline the need for further research that includes expanded sampling from different regions and cattle breeds and the application of varied diagnostic methods. Such studies will contribute to a more comprehensive understanding of the genetic diversity, distribution, and epidemiology of E. bovis and E. zuernii in Indonesia, supporting improved cattle health and management practices in the region.
Repellent Power of Kuit Lime Peel Ethanol Extract (Citrus amblycarpa L.) Against Aedes aegypti Mosquitoes Azzahra, Farah Nabila; Hastutiek, Poedji; Lazuardi, Mochamad; Suprihati, Endang; Kurnijasanti, Rochmah; Sunarso, Agus
Journal of Parasite Science Vol. 9 No. 2 (2025): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v9i2.64904

Abstract

This research investigated the capability of the kuit lime peel ethanol extract as a repellent against Aedes aegypti mosquitoes. This study used the post test only control group design approach and the experimental method recommended by the World Health Organization Pesticides Evaluation Scheme (WHOPES) with modification. This study used seven different experiment groups consisting of a negative control group using a mixture of Tween 20 and aquadest, a positive control group using commercial mosquito repellent (Autan®), and concentration groups using 5%, 15%, 30%, 45%, and 60% (m/v) of the kuit lime peel ethanol extract. Each experiment groups were applied on a volunteer’s arms that are then inserted in a testing cage containing 25 female adult Ae. aegypti mosquitoes. Every mosquito that landed on the arm testing area will be calculated during the examination time (every 2 min for 30 min) to determine the repellent protection power percentage. Based on the result of the study using Two-Way Analysis of Variance and Duncan’s Multiple Range Test, the kuit lime peel ethanol extract is confirmed to be the most effective as a repellent against Ae. aegypti mosquitoes at the 30% concentration and until the 29th minute post-application with a 100% protection power.
Prevalence of Hymenolepis nana in wild rats in Surabaya City, East Java Dwi Putri Rahmawati; Suwanti, Lucia Tri; Mufasirin; Endang Suprihati; Poedji Hastutiek; Kusnoto; Heni Puspitasari; Alasrorik, Muhammad Hizbulloh
Journal of Parasite Science Vol. 9 No. 2 (2025): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v9i2.65448

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Hymenolepiasis is one of the neglected zoonotic diseases in humans, caused by the cestodes Hymenolepis nana and Hymenolepis diminuta. Its definitive hosts are rodents (mostly rats). This study aims to identify the morphology both microscopically and macroscopically. This research is a laboratory exploratory research and the sampling method uses purposive sampling technique. The samples used in this study amounted to 100 samples of wild rats that had been trapped and then necropsied, worms obtained from the digestive tract were stained with dark red stain and observed with a microscope. The results showed that microscopic morphological examination showed that the anterior part of the H. nana worm had hooks and crowns. The overall prevalence of H. nana worms was found to be 18% (18/100). The prevalence of H. nana in wild rats in Surabaya City in Rattus tanezumi was 16% (12/77). Rattus novergicus in Surabaya area with positive samples was 26% (6/23). Morphology in H. nana worms has a distinctive difference. The anterior part of H. nana has hooks that surround the rostellum, the anterior part has a ‘crown of hooks’.
Detection of Cryptosporidium spp. in Wild Rats (Rattus spp.) in Surabaya, East Java Pratama, Ponasari Galuh; Suprihati, Endang; Suwanti, Lucia Tri; Mufasirin; Hastutiek, Poedji; Setiawan, Boedi; Puspitasari, Heni; Indasari, Elly Nur
Media Kedokteran Hewan Vol. 35 No. 2 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i2.2024.71-78

Abstract

Cryptosporidiosis is a waterborne zoonosis caused by Cryptosporidium, which is a parasite that causes infection of the small intestine and leads to acute diarrhea in both humans and animals. Wild rats (Rattus spp.) are known to carry many zoonotic pathogens including Cryptosporidium. Given their close proximity to humans in urban environments, the likelihood of disease transmission is high. This study aims to detect the Cryptosporidium spp. infection in wild rats in Surabaya City, East Java Province. Intestinal fecal samples were collected using necropsy. The flotation test and Ziehl-Neelsen stain were used to observe the presence of Cryptosporidium under the microscope. The results showed that 69 samples were positive for Cryptosporidium spp. in the oocyst stage, which appeared dark pink with a clear cavity and the size of the oocysts ranged from 2-6 μm. The high incidence of cryptosporidiosis in wild rats is a potential health threat to both animals and humans.
Epidemiological Studies of Subclinical Mastitis in Dairy Goats in Lumajang Regency, East Java, Indonesia Fatmawati, Mira Fatmawati; Lucia Tri Suwanti; Mufasirin; Mirni Lamid; Nunuk Dyah Retno Lastuti; Endang Suprihati; Mustofa Helmi Effendi; Anam Al Arif; Widi Nugroho; Masdiana C Padaga
Jurnal Ilmu-Ilmu Peternakan Vol. 33 No. 3 (2023): December 2023
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2023.033.03.07

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Subclinical mastitis is an economically important disease in dairy goat farming. Lumajang is a center for dairy goats in East Java. However, routine examination of subclinical mastitis for screening tests has no data. This study aimed to provide prevalence data and identification factors that influenced subclinical mastitis. Therefore, this study was carried out as part of the national surveillance program. The research design is a cross-sectional study with simple random sampling. Samples came from 19 farms with a total individual sample of 224 dairy goats. Detection of subclinical mastitis using an undirect test with the California Mastitis Test (CMT). Identification of management factors and characteristics that can influence subclinical mastitis in dairy goat farms using a structured questionnaire. The results showed that the prevalence of subclinical mastitis in Lumajang District was 36.2%. There was a significant difference in subclinical mastitis in each village (p<0.05). The highest prevalence of subclinical mastitis was in Kandangtepus village. A farmer's characteristic factor that significantly influences subclinical mastitis is the farmer's experience. The aspect of livestock management that significantly affects subclinical mastitis is structured work management. Subclinical mastitis was prevalent in dairy goats in Lumajang District and requires prevention efforts. Prevention approach through education for farmers to increase knowledge, attitude, and socialization to improve livestock management.
PENGARUH pH LENDIR MUKOSA VAGINA SAAT BIRAHI TERHADAP PERSENTASE KEBUNTINGAN (Conception Rate) PADA SAPI PERAH DI KUD TANI WILIS KABUPATEN TULUNGAGUNG DAN KSU TUNAS SETIA BARU KABUPATEN PASURUAN Rizki, Andaru; Srianto, Pudji; Suprihati, Endang; Sardjito, Trilas; Ismudiono, Ismudiono; Al Arif, Mohammad Anam
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.312 KB) | DOI: 10.20473/ovz.v8i2.2019.154-158

Abstract

The purpose of this research is to determine the effect of mucos acidity of vaginal mucous when estrus towards conception rate at KUD Tani Wilis Tulungagung District and KSU Tunas Setia Baru Pasuruan District. Measured samples were taken from 30 dairy cattles in each KUD. Mucos acidity of vaginal mucous were obtained by using a digital acidity meter before insemination was done on dairy cattle. After measuring the acidity from mucosal vagina mucous, artificial insemination has done on estrus dairy cattle. On the 60th day after insemination, rectal palpation was done to check the conception rate. Research result from 30 dairy cattle on each KUD showed that mucos acidity of vaginal mucous has signifficant effect towards conception rate. Mucos acidity of vaginal mucous range 6.5-8.5 with the highest range of conception rate in KSU Tunas Setia Baru Pasuruan District 7.5-7.7 and in KUD Tani Wilis Tulungagung District with the highest range of conception range 7.2-7.4 and 7.5-7.7.
Angka kebuntingan sapi perah dengan korpus luteum persisten setelah pemberian kombinasi prostaglandin F2α dan gonadotropin Azwani, Nabila; Srianto, Pudji; Madyawati, Sri Pantja; Sardjito, Trilas; Suprihati, Endang
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 2 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i2.2021.59-64

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This case study aims to determine the effect of a combination of prostaglandin F2α (PGF2α) with Gonadotropin (PG-600), and human chorionic gonadotropin (hCG) on the estrus and pregnancy rates in Holstein Friesian (HF) cows with persistent corpus luteum (PCL). A total of 15 Holstein Friesian (HF) dairy cows aged 3-5 years with body condition score (BCS) 5-7 (scale 1-9) diagnosed with PCL, were injected with 7.5 mg PGF2α intra-vulvar submucosa. Cows were then divided into three treatment groups. Group P0 was not given the combination treatment, while P1 group was injected with a  combination of 300 IU PG-600 and 150 IU hCG, meanwhile P2 group was was injected with a combination of 300 IU PG-600 and 300 IU hCG intra-muscularly. The PGF2α and PG-600 injections were carried out on the same day PCL was diagnosed. Meanwhile, the injection of hCG was carried out at the same time as the AI. The Pregnancy examination was performed 90 days after AI through rectal palpation. The results showed that all treated cows were estrus  observed with the fastest onset of estrus was the P2 group, that was significantly different (p <0.05) compared to the P0 and P1 groups. All treated cows were diagnosed as pregnant. It can be concluded that the administration of PGF2α or its combination with gonadotropins in HF cows with PCL could result in 100% estrus and pregnancy rates.
Co-Authors Adi Sofyan Ansori, Muhammad Afiyah Rahmadani Agnes Theresia Soelih Estoepangestie Akbar Wijaya Putra Purnama Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Alasrorik, Muhammad Hizbulloh Aldin Akbar Rahmatullah Amara Lintang Pagati Amelia Dwita Safitri Anam Al Arif Anggreani Desi Ramadhani Rahajeng Arif Pratiwi Arimbi Arimbi Ayu, Zerlinda Dyah Azizah Bilqis Nurkarimah Azwani, Nabila Azzahra, Farah Nabila Boedi Setiawan Cindy Ercha Aulia Putri Dadik Rahardjo, Dadik Dadik Raharjo David Mohamad Qadafi Dhio Asmaydo Dian Ayu Permatasari Didik Handijatno Doohan Mahendra Doohan Mahendra DWI PUTRI RAHMAWATI Dyah Ayu Kurniawati Dyah Ayu Kurniawati Edward Yonas Kristijanto Ellza Agatha Damayanti Emy Koestanti Sabdoningrum Eny Coolfina Simarmata Erma Safitri Fadila Zikri Amanda Faisal Fikri Fatmawati, Mira Fatmawati Fedik Abdul Rantam Firman Hadi Fanani Fransiska Cicilia Beka Ghifari Lutfi Fauzi Hana Eliyani Hani Plumeriastuti Hartono Hartono Hayuning Nurrodhiya Heni Puspitasari Imam Mustofa Indasari, Elly Nur Ismudiono Ismudiono, Ismudiono Iwan Sahrial Hamid Iwan Sahrial Hamid Jihaan Haajidah Khairullah, Aswin Rafif Kiki Amalia Rama Koesdarto Koesdarto Kuncoro Puguh Santoso Kurniawan, Muhammad 'Ahdi Kurniawati, Dyah Ayu Kusnoto Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Kusriningrum Rochiman Legowo, Djoko Lianny Nangoi Lilik Maslachah Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Marchelia Arifiandani Masdiana C Padaga Maudika Pamela Fiorenza Meta Aprilia Meyreta Doti Alcaterana Mirni Lamid Mochamad Lazuardi Mohammad Sukmanadi Moses, Ikechukwu Benjamin Mufasirin Muhammad Fahmi Abdillah Muhammad Hambal Mustofa Helmi Effendi Nenny Harijani Ni Komang Aprilina Widisuputri Nidom, Chairul Anwar Niluh Selly Frantika Nining Sari Virgandina Vinola Nizar Bachrudin Prihandono Nunuk D.R. Lastuti Nunuk Dyah Retno Lastuti Nunuk Dyah Retno Lastuti Nurin Ardhiani Panjaitan, Cahaya Poedji Hastutiek Praja, Ratih Novita Pratama, Bima Putra Pratama, Ponasari Galuh Pratiwi, Arif Prawasa, Ramadhiniyanti Putri Alif Pudji Srianto Puput Ade Wahyuningtyas Puput, Sesa Rahmi Sugihartuti Rahmi Sugihartuti, Rahmi Ratna Damayanti Retno Wulansari Riwu, Katty Hendriana Priscilia Rizki, Andaru Rochmah Kurnijasanti Romy Muhammad Dary Mufa Sandy, Berliana Dwi Nandita Sarmanu, Sarmanu Setiawan Koesdarto Setiawan Kusdarto Soeharsono Soeharsono Sri Mumpuni Sosiawati Sri Pantja Madyawati Sunarso, Agus Supranianondo, Koesnoto Suzanita Utama Tjuk Imam Restiadi Trilas Sardjito Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widi Nugroho Widodo, Oky Setyo Wiwik Misaco Yuniarti Yudhana, Aditya Yulianna Puspitasari Yunus, Muchammad