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All Journal Jurnal Gizi dan Pangan Nexus Kedokteran Komunitas Nexus Kedokteran Klinik Nexus Biomedika Nexus Kedokteran Translasional Jurnal Media Gizi Indonesia (MGI) MNJ (Malang Neurology Journal) The Indonesian Biomedical Journal Jurnal Gizi dan Kesehatan Universa Medicina Paediatrica Indonesiana Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Ilmu Gizi Indonesia Jurnal Gizi dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics) Indonesian Journal of Medicine Jurnal Aisyah : Jurnal Ilmu Kesehatan Nusantara Science and Technology Proceedings PROFESI (Profesional Islam): Media Publikasi Penelitian INPHARNMED Journal (Indonesian Pharmacy and Natural Medicine Journal) Narra J JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Indonesian Journal of Cancer Prosiding University Research Colloquium The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy Journal of Advanced Technology and Multidiscipline (JATM)
Brian Wasita
Fakultas Kedokteran, Universitas Sebelas Maret
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Dentistry Economics, Econometrics & Finance Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary Other

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Red Rice Bran Ethanol Extract Reduces IL-1β as the Risk of Pancreas Fibrogenesis in Type 2 Diabetic Rat Model Wasita, Brian; Yudhani, Ratih Dewi; Pakha, Dyonisa Nasirochmi; Nurrohima, Diana; Muthmainah, Muthmainah; Wiyono, Nanang; Fakurazi, Shariza
The Indonesian Biomedical Journal Vol 17, No 1 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i1.3410

Abstract

BACKGROUND: Oxidative stress and inflammation contribute to pancreatic cell dysfunction that promote insulin resistance in type 2 diabetes (T2D). Red rice bran contains bioactive substances with anti-inflammatory and antioxidant properties which improved insulin resistance in obese mice. However, no studies have explored the potential of ethanol extract of red rice bran (EERRB) to prevent T2D progression, particularly pancreatic fibrosis complications. This study was conducted to investigate the effect of EERRB on inflammation measured with interleukin (IL)-1β and fibrosis of pancreatic tissue in a rat model of T2D. METHODS: Rats were induced with streptozotocin and nicotinamide to induce diabetes, and then separated into five groups. One group received no treatment, while the other four received 9 mg/kg/day acarbose, 165, 330, or 660 mg/kg/day EERRB orally for 21 days. Immunohistochemistry was conducted on pancreas tissues to measure the expression of IL-1β, while pancreatic fibrosis was assessed with Masson’s Trichrome staining.RESULTS: EERRB reduced the expression of pro-inflammatory cytokine, IL-1β, in pancreas tissue in a dose dependent manner. Significantly lower IL-1β expression were found in group receiving 660 mg/kg/day EERRB (10%) compared to diabetic with no treatment group (50%) (p<0.0001). Additionally, the IL-1β expression in the highest dose of EERRB group was comparable to the group receiving acarbose (10%). CONCLUSION: This finding suggests the beneficial effect of EERRB in the hyperglycemic condition that causes oxidative stress through blocking the IL-1β expression, hence alleviating the inflammation in pancreas tissue, and have a tendency in preventing pancreatic fibrosis progression, a process implicated in T2D pathogenesis. KEYWORDS: diabetes, inflammation, pancreatic fibrosis, red rice bran
Retinoblastoma tumor suppressor protein is not a risk factor for the location of condyloma acuminatum among adults Primisawitri, Pratiwi Prasetya; Mulianto, ⁠Nurrachmat; Ellistasari, Endra Yustin; Poncorini, Eti; Kariosentono, ⁠⁠Harijono; Widhiati, Suci; Wasita, Brian; Setyawan, Novan Adi; Yudhistira, Muhammad Yurizar
Universa Medicina Vol. 44 No. 3 (2025)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2025.v44.343-349

Abstract

BackgroundCondylomata acuminata (CA) are sexually transmitted infections primarily caused by low-risk HPV types 6 and 11. Retinoblastoma protein (pRb) interacts with HPV oncoproteins, leading to destabilization, genomic instability, and carcinogenesis. This study aimed to determine whether variations in pRb expression and sexual orientation are risk factors of the anatomical location of CA lesions and to compare differences in pRb expression by sexual orientation. MethodsA cross-sectional study was conducted among 33 patients aged >18 years with clinically and histopathologically confirmed CA. Demographic data, pRb expression assessed via excision biopsy and immunohistochemistry, and relevant risk factors were collected. Statistical analyses including Simple and multiple binary logistic regression were used to analyze the data, with model fit assessed using the Hosmer–Lemeshow test. ResultsWeak pRb expression predominated in anal lesions (92.3%; p=0.018) and among homosexual/bisexual individuals (57.9%; p=0.031), while moderate expression was more common in genital lesions (66.7%; p=0.018) and heterosexual individuals (57.1%; p=0.011). Simple logistic regression  analysis showed that HIV status (OR=11.88; p=0.025), sexual orientation (OR=13.33; p=0.001), and moderate-to-strong pRb expression (OR=24.00; p=0.008; OR=12.00; p=0.048) were associated with lesion location. In the multivariate model, pRb expression was excluded due to multicollinearity. Sexual orientation remained the only independent predictor of lesion location (Adj OR=8.89; p=0.023), with heterosexual individuals more likely to present with genital lesions. ConclusionThe expression of pRb was not a risk factor of the anatomical location of CA, and differed between genital and anal CA. However, sexual orientation emerged as the dominant independent factor associated with lesion location.   
PERUBAHAN BERAT BADAN TIKUS AKIBAT INJEKSI HOMOSISTEIN SEBAGAI MODEL HEWAN PENYAKIT ALZHEIMER Rizma, Amalina; Wasita, Brian; Probandari, Ari
INPHARNMED Journal (Indonesian Pharmacy and Natural Medicine Journal) Vol. 5 No. 1 (2021)
Publisher : Alma Ata University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21927/inpharnmed.v5i1.1630

Abstract

Homosistein merupakan salah satu senyawa yang digunakan untuk menginduksi penyakit alzheimer pada tikus. Kadar homosistein yang tinggi memicu stress oksidatif, peradangan saraf dan pembentukan amiloid-beta pada otak. Hal ini dapat mempengaruhi fungsi otak yang terlibat dalam pengaturan nafsu makan sehingga dapat mengakibatkan penurunan berat badan. Penelitian ini bertujuan untuk menganalisis perubahan berat badan tikus yang diinjeksi homosistein selama 7 hari, 14 hari dan 21 hari. Sebanyak 9 ekor tikus galur Sprague dawley usia 8-12 minggu dengan berat 150-200gram dibagi menjadi 3 kelompok. Kelompok I, II dan III diinjeksi homosistein masing-masing selama 7, 14 dan 21 hari. Berat badan tikus ditimbang sebelum perlakuan, hari ke-7, hari ke-14 dan hari ke-21 setelah pemberian homosistein. Analisis data menggunakan uji paired t-test dan one-way Anova. Rata-rata berat badan tikus kelompok I, II dan III mengalami peningkatan masing-masing sebesar 3,33±1,15 gram; 5,00±2,00 gram; dan 2,33±3,51 gram. Hasil analisis statistik menunjukkan bahwa terdapat perbedaan berat badan yang signifikan antara sebelum dan sesudah injeksi homosistein selama 7 hari pada kelompok I (p=0,038) dan 14 hari pada kelompok II (p=0,049), sedangkan berat badan tikus pada kelompok III tidak mengalami perubahan yang signifikan (p=0,369). Tidak ada perbedaan berat badan yang signifikan antara kelompok I, II dan III setelah injeksi homosistein (p=0,182). Berat badan tikus model alzheimer setelah injeksi homosistein selama 7, 14, dan 21 hari mengalami perlambatan kenaikan berat badan. Kondisi ini mirip dengan pasien alzheimer yang pada umumnya mengalami penurunan berat badan sehingga perlu pemantauan asupan makan untuk mencegah penurunan berat badan lebih lanjut.
PENGARUH HOMOSISTEIN TERHADAP BERAT OTAK TIKUS MODEL PENYAKIT ALZHEIMER Marintan, Elita; Wasita, Brian; Magna, Adi
INPHARNMED Journal (Indonesian Pharmacy and Natural Medicine Journal) Vol. 5 No. 1 (2021)
Publisher : Alma Ata University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21927/inpharnmed.v5i1.1765

Abstract

Homosistein (hcy) adalah asam amino mengandung sulfur yang terbentuk selama metabolisme metionin asam amino esensial. Pemberian homosistein menyebabkan peningkatan stres oksidatif, kerusakan DNA, pemicu apoptosis dan eksitotoksisitas, yang penting dalam degenerasi saraf. Kadar homosistein yang meningkat menyebabkan neurotoksisitas dan atrofi otak pada Penyakit Alzheimer (AD). Penelitian ini bertujuan untuk mengamati bobot otak tikus Sprague Dawley yang diinduksi Hcy selama 7, 14 dan 21 hari. Penelitian ini merupakan eksperimental laboratorik dengan posttest only group  design. Sembilan ekor tikus Sprague dawley umur 8-12 minggu dengan berat badan antara 150-200 gram dibagi secara acak menjadi 3 kelompok (n=3). Semua kelompok diberikan injeksi homosistein dengan dosis yang sama yaitu 0,4 mg / kg berat badan. Kelompok I, II dan III diinjeksi homosistein selama 7, 14, dan 21 hari untuk setiap kelompok secara berurutan. Pengamatan berat otak dilakukan setelah eutanasia pada hari ke 7, 14, dan 21 setelah perlakuan. Sampel berat otak diukur dengan menggunakan timbangan digital. Perbedaan berat otak antar kelompok dianalisis dengan menggunakan ANOVA. Hubungan antara berat otak dan lama injeksi homosistein dianalisis dengan menggunakan uji korelasi product-moment Pearson. Semua prosedur penelitian telah dilakukan dengan persetujuan dari Komite Etik Hewan Fakultas Kedokteran UNS No: 106/UN27.06.6.1/KEPK/EC/2020. Injeksi homosistein selama 7, 14 dan 21 hari tidak mengubah berat otak. Berat otak mengalami penurunan tetapi tidak signifikan secara statistik  (p=0,549). Hasil penelitian menunjukkan bahwa peningkatan kadar homosistein tidak mempengaruhi berat otak tikus model penyakit Alzheimer.
Potential of avocado oil (Persea americana) in improving triglyceride and High Density Lipoprotein (HDL) levels in rats (Rattus novergicus) model of dyslipidaemia due to high fat diet Sari, Eka Puspita; Wasita, Brian; Muthmainah, Muthmainah
Jurnal Gizi dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics) VOLUME 12 ISSUE 4, 2024
Publisher : Alma Ata University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21927/ijnd.2024.12(4).296-305

Abstract

Background: Elevated levels of Dyslipidaemia pose a significant threat to cardiovascular health. Fortunately, the abundance of polyunsaturated and monounsaturated fatty acids (PUFA and MUFA) present in avocado oil offers a promising avenue for individuals with Dyslipidaemia to improve their lipid profile levels.Objectives: The objective of this research is to ascertain the potential of avocado oil in improving triglyceride and HDL levels.Methods: A pre-post test control group design was employed, utilizing male Wistar rats aged 8 weeks with a body weight ranging from 150-200 g. The mice were segregated into six groups: N, K-, K+, P1, P2, and P3. Throughout the trial, solely group N abstained from ingesting avocado oil or HC. Conversely, all other groups were administered HC and subsequently, K+ was prescribed simvastatin while P1, P2 and P3 received avocado oil for a duration of 28 days. The data was subjected to statistical analysis using SPSS vr.25. The statistical significance threshold was set at p<0.05.Results: Mean changes in levels (mg/dL) for TG -29.67 (P1), -48.99 (P2), -56.78 (P3) and for HDL 41.75 (P1), 46.35 (P2). There was a significant difference in TG and HDL levels between P1, P2, P3 when compared with K- (p<0.05).Conclusion: Avocado oil exhibits efficacy in lowering TG levels and elevating HDL levels, rendering it a viable option for daily consumption.
Co-Authors Adi Magna, Adi Adi Prayitno Afdhaliya, Noor Khalwati Agus Kristiyanto Amarduan, Tince Bety Amilia Yuni Damayanti Ana Rima Ari Probandari Asrial, An A. Bambang Purwanto Binti Maulina Budiningsih, Fatichati Budiyanti Wiboworini Cindikya Saftiari Dewi Cr. Siti Utari Cr. SitiUtari Darmayani, Aritantri David Perdana Dentiko Wasis Aulia Dewi Okta Anggraini Diana Nurrohima Didik Prasetyo Diffah Hanim Dono Indarto Dyonisa Nasirochmi Pakha Eka Puspita Sari Ellistasari, Endra Yustin ernawati, ammik Eti Poncorini Pamungkasari Fadila, F Fakurazi, Shariza Gardjito, Fajar Baskoro Gunawan, Timotheus A. Hari Wujoso Harsono Salimo Hermansyah, Muhamad Lukman Herviana, Herviana Ida Nurwati Idulhaq, Mujaddid Irma Suryani Jati, Agus Kariosentono, ⁠⁠Harijono Kusumaningrum, Sulistyani Laqif, Abdurrahman LAWEI, THARINA Marintan, Elita Muchamad Syamsulhadi, Muchamad Muhammad Riza Mulianto, ⁠Nurrachmat Muthmainah Muthmainah Muthmainah Nanang Wiyono Nofia, Yasinta Novan Adi Setyawan Nur Shani Meida Nursulistyo, Fajar Paramasari Dirgahayu Paulus Kusnanto Pesik, Riza N. Poncorini, Eti Primisawitri, Pratiwi Prasetya Pudjiastuti, Anggit puguh santoso, puguh Rachmi Fauziah Rahayu Rahayu, Rachmi F. Rahmah Rahmah Ratih Puspita Febrinasari Reviono Reviono Risya Cilmiaty, Risya Riza Deviana Riza Novierta Pesik Rizma, Amalina Sabariyah, Lely Santi, Nur Saputra, Rhyan Darma Senyum Indrakila Setianto, Budi Y. Soestrisno, Soestrisno Soetrisno Soetrisno Soetrisno Suyatmi Suyatmi Tamaulina Br Sembiring Taufiqurrahman, Surya Tedjo Danudjo Tonang Dwi Ardyanto Tri Harsono Tri Nugraha Susilawati Triyanta Yuli Pramana Umi Mahmudah Vitri Widyaningsih Wardhani, Lusi O. Wayanshakty, Janur Widhiati, Suci Wulandari, Eka T. Wulansari, Indry Yarsa, Kristanto Y. Yudhani, Ratih D. Yudhani, Ratih Dewi Yudhistira, Muhammad Yurizar Yundari, Yundari yusari Yusup Subagio Yusup Subagio Sutanto Zahidah, Fairuz Zaidah, Lailatuz