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Journal : Universa Medicina

Rapid isolation and characterization of Wharton's jelly-derived mesenchymal stem cells maintained in fresh-prepared human AB-serum Tjahjono, Yudy; Dewi, Sianty; Novita, Bernadette Dian; Wijaya, Hendy; Putra, Brilliant Dwi; Kuncorojakti, Suryo; Hendriati, Lucia; Jong, FX Himawan Haryanto; Widodo, Teguh; Malonda, Franklin Vincentius
Universa Medicina Vol. 44 No. 1 (2025)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2025.v44.65-72

Abstract

Background Mesenchymal stem cells (MSCs) are valued in regenerative medicine for their multipotency, proliferative capacity, and immunomodulatory properties. Wharton’s jelly-derived MSCs (WJ-MSCs) from the umbilical cord offer a non-invasive, promising source for clinical applications, because easy isolation, lack of ethical concerns, and the presence of both embryonic and adult stem cells have made them a valuable source for use in therapeutic applications and regenerative medicine. This study aimed to optimize WJ-MSC isolation and characterization methods. Methods Human umbilical cords from three healthy donors were collected post-cesarean under strict inclusion criteria. WJ-MSCs were isolated using the explant culture method, with cells adhering to T75 flasks pre-coated with 2% gelatin. Cultures were maintained in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% freshly prepared Human AB serum and monitored for 21 days. Flow cytometry (BD FACSAria) was performed at passages 1 and 5 to assess MSC markers CD105, CD73, CD90, and CD44, alongside the exclusion marker CD45. Results WJ-MSCs exhibited fibroblast-like morphology by passage 1 and showed robust proliferation. Flow cytometry revealed high CD44 expression (~60%) at passage 1, while CD105, CD73, and CD90 became prominent by passage 5. CD45 remained low, suggesting minimal hematopoietic contamination. Conclusion This study confirms the feasibility of isolating and expanding WJ-MSCs using DMEM with 10% human AB serum. While consistent cell growth was achieved, the 21-day culture period may require optimization for scalability, including serum concentration, substrate coatings, and oxygen levels. CPJ-MSCs may be preferable for applications demanding rapid expansion and early marker expression.
Co-Authors Abrar, Muhammad Bintang Achmad Hidir Achmad Manshur Ali Suyanto Agus Ganda Permana Agus Setia Ningsih Agustina K, Tin Ahab, Julen Jessica Ahmad Mahmuda Aini, Fica Aida Nadhifatul Alfarobi, Muhammad Luthfi Alni Fitri Amalia, Rika Andrian Hermawan Angelica Kresnamurti Anrika, Merry Yulia Ardianto, Andi As Shalikhah, Sinta Wahidha Asep Suhendi Asep Syarip Hidayat Asmar, Alamuddin Al Asri Laksmi Riani Aulia, Nurul Quraini Aurelita, Feiza Ayu Liskinasih Ayu, Nur Azan Rahmat Muhammad Azmi Rahman Bila Wibowo, Salsa Bimo Satrio Wibowo Budi Ardianto Budi Leksono Cahyadi, I Putu Erry Chandra, David Kurnia Chintya Putri Wardani Citra Zita Hermeindita Daffa Zakaria Damayanti Siburian, Alfina Devina Jocelin Handoko Dewantara, Dino Dewi Susilawati Dewi, Liza Astriana Dewi, Widi Dian Kurnia Anggreta Diana, Nur Ayu Diva Septina Manik Domos, Erma Dwi Juniar Putri Sitorus Dwi Nita Aryani Efendi , Erfan Elizabeth Natania Ivani Emia Rehjorena br Sihite Fadlurachman, Reihan Fanny khazanah Farin Fitri Syahdila Fatchurrohman, Rois Muflich Fatmawati, Alip Fatsyahrina Fitriastuti Fauzan, Ariq Fikri Filda Fransisca Franssisca, Franssisca Gilang, Yohanes De La Cruz Guruminda, Trisna Gustiani, Eva Hafidudin . Hafizah, Nurul Heikal, Jerry Heriska, Hanny Herlanda, Nandika Rahadian Heru Dradjat Sulistyo Hesti Asriwandari Ibra Wa Ikhsan, Maimubdi Ifada, Shafa Radisya Aulia Imami, Sulfia Indriyati Hadi Sulistyaningrum Ivani, Elizabeth Natania Iwan Sahrial Hamid Jong, FX Himawan Haryanto K, Tin Agustina Kariyaningtias, Vivi Karnadi, Karnadi Kresnamurti, Angelica Kuncorojakti, Suryo Lannie Hadisoewignyo Larasati, Annisa Lisa Putri Yayanda Lucia Hendriati Lucia Hendriati Malonda, Franklin Vincentius MAYA, IRA Mayka, Febe Meridayanti, Meridayanti Merry Yulia Anrika Mochamad Syamsiro Muchammad Ajhi Romadhon Muhammad Iqbal Muhammad Mahesa Ramadhan Muhammad Royan Hidayat Muhammad, Fuad Asrofillah Muhardi, M. Mulkan Azhiman Multani, Gemilang Musafak, Moh Nanda Wulandari, Nanda Niken Paramita Nisa, Ardini Khairun Novi Erdila Novi Prihatiningrum Novita, Bernadette Dian Nst, Indra Budiman Nur Azmi Ainul Bashir Nur Wening, Nur Nurchairunisyah, Nurchairunisyah Oktaviani Meri Syaputri Perdana, Mohammad Alkadri Pradela, Yunia Prasojo Pribadi Putra, Brilliant Dwi R, Widi Dewi Rahma Yuli Suciya Redah Jeiyusman Resdati Ridho Adjie Pratama Rina Susanti Rio Samuel Risdayati, Risdayati Rizaldi, Muhammad Rizaldi, Muhammad Rizky Abet Panjaitan Robi Armilus, Robi Ruspitasari, Widi Dewi Ryan Ari Setyawan Ryan Mustafa Kamal Safitri Saifuddiin, Muhammad Rizqi Salam, M. Fathan Sari Romaida Sihite Seri Wahyuni Setijadi, Veronica Sianty Dewi Simaremare, Tohap Pandapotan Simarmata, Agnes Dewina Sinta, Sri Slamet . Soeparto Soeparwoto Dharmoputra Solikhah, Mar’atus Soni Tia Kurniawan Sony Sumaryo Sovia, Nani Sri Juniarti Suhartatik Sulih Indra Dewi, Sulih Indra Sundari, Melda Suryadi Suryadi Suryawati, Porman Ida Susanti, Ena Suseno, Bayu Susi Susanti Susmanto, Prahady Syafawani, Rufaidah Syah, Mohammad Prima Aldebaran Syam, Syahrul Syamsul Bahri Syawaludin, Rahmat Tarra, Mustika Tasya, Atiya Tiara, Beby Tin Agustina Karnawati Tjahjono, Yudy Tjay, Stephanie Tomu Yupiter Situngkir Tri Atmojo, Jose Juang Vernanda, Salsabilla Visencia, Novita Waldi Herwanda Wardiana, Irna Wijaya, Hendy Willyanto, Jennifer Rachel WINANTI, WINANTI Wulandari, Naomi Yahya Arwiya Yanuar Herlambang Yessi Kartalina Yosi Suwarni Yoskar Kadarisman Yulias, Putri Oktaviani Yunengsih, Yayu Yuniar Fitriyani Yusmar Yusuf Zallen, Adyta Zusana E. Pudyastuti