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Efek Pemberian Ekstrak Daun Sungkai (Peronema canescens Jack) Terhadap Proliferasi dan Migrasi Sel Kanker Serviks HeLa Rahmawati, Meilisa; Arisanty, Dessy; Rahmadian, Rizki; Yerizel, Eti; Rusjdi, Dina Arfiani; Rahmatini, Rahmatini
Majalah Kedokteran Andalas Vol. 47 No. 4 (2024): MKA October 2024
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v47.i4.p383-398.2024

Abstract

Tujuan: mengetahui pengaruh pemberian ekstrak daun sungkai dalam menghambat proliferasi dan migrasi sel HeLa. Metode: Penelitian ini merupakan penelitian eksperimental yang menggunakan 24 disk kultur sel HeLa yang konfluens. Sampel dibagi menjadi 4 kelompok (K,P1,P2 dan P3). Kelompok perlakuan diberikan ekstrak daun sungkai dengan konsentrasi IC25, IC50, dan IC75 yang diinkubasi selama 72 jam. Analisis data menggunakan uji One Way ANOVA dan Post Hoc Bonferroni. Hasil: Rerata jumlah sel proliferatif yang didapatkan pada kelompok K, P1, P2, dan P3 berturut-turut adalah 12,1 x 105, 8,94 x 105, 6,14 x 105 dan 5,04 x 105. Rerata migrasi jumlah sel yang berkumpul pada zona scratch pada kelompok K, P1, P2, dan P3 berturut-turut adalah 49,.0, 237,8, 210,5, dan 100,7. Didapatkan perbedaan yang bermakna tiap kelompok pada uji proliferasi dan migrasi dengan nilai p = 0,000 (p < 0,05). Kesimpulan: Terdapat pengaruh pemberian ekstrak daun sungkai terhadap proliferasi dan migrasi sel HeLa. Perbedaan bermakna ditemukan pada kelompok kontrol dan kelompok perlakuan dengan konsentrasi ekstrak IC25, IC50, dan IC75.
Pengaruh Pemberian Ekstrak Daun Kelor (Moringa Oleifera) Sebagai Antioksidan Terhadap Kadar Malondialdehyde Pada Tikus Model Hiperglikemia Sari, Asti Marian; Yerizel, Eti -; karmia, Hudila Rifa
Majalah Kedokteran Andalas Vol. 48 No. 1 (2025): MKA January 2025
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v48.i1.p63-70.2025

Abstract

Tujuan: Mengetahui pengaruh pemberiak ekstrak Moringa Oleifera terhadap kadar malondialdehyde pada tikus wistar model hiperglikemia. Metode: Penelitian ini melibatkan 20 ekor tikus strain wistar betina yang berumur 8-12 minggu dengan bobot 200-250 gram yang memenuhi kriteria inklusi maupun ekslusi. Tikus dibagi menjadi kelompok empat, yaitu Kelompok kontrol negatif (K-), Kontrol positif (K+), Perlakuan 1 (P1) dan Perlakuan 2 (P2). Kelompok P1 hanya diberikan makan dan minum standar saja, tanpa perlakuan. Kelompok K+ diinjeksi aloksan secara Intraperotoneal dengan dosis 100 mg/kgBB sehingga tikus menjadi hiperglikemia, P1 diberi injeksi aloksan menjadi hiperglikemia dan diberikan ekstrak moringa oleifera dengan dosis 250 mg/kgBB dan P2 injeksi aloksan menjadi hiperglikemia dan diberikan ekstrak moringa oleifera dengan dosis 500 mg/ kgBB selama 21 hari, Pada hari ke-22 semua kelompok diambil darah untuk dilakukan pemeriksaan kadar malondialdehyde. Hasil: Rerata kadar malondialdehid pada K-, K+, P1 dan P2 berturut-turut, yaitu 1,81±0,06 nmol/mL, 3,81±0,24 nmol/mL, 2,55±0,30 nmol/ mL, 2,09±0,21 nmol/mL, dengan nilai p=0,01. Kesimpulan : Pemberian ekstrak moringa oleifera sebagai antioksidan berpengaruh terhadap penurunan kadar malondialdehid
Uji Efektifitas Antioksidan Minyak Zaitun terhadap Malondialdehyde (MDA) pada Tikus Hiperglikemia Okvenda, Ariani Zaltin; Yerizel, Eti; Yerizel, Raveinal; Yerizel, Almurdi
Jurnal Biomedika dan Kesehatan Vol 6 No 2 (2023)
Publisher : Fakultas Kedokteran Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/JBiomedKes.2023.v6.158-169

Abstract

Background Hyperglycemia is a medical condition that increases blood glucose levels beyond normal limits because the body lacks enough insulin or insulin does not work properly and is known to trigger oxidative stress. In cells sensitive to Hyperglycemia, such as endothelial cells, excessive glucose loads trigger the formation of Reactive Oxygen Species (ROS) in mitochondria, impairing mitochondrial function. ROS are essential mediators for activating pro-inflammatory signaling pathways; obesity and hyperglycemia-induced ROS production may support the induction of proinflammatory macrophages such as M1 during the onset and development of diabetes. Consumption of olive oil can increase plasma antioxidant capacity and reduce oxidative stress markers. Methods This study aimed to determine the effectiveness of olive oil antioxidants against Malondialdehyde (MDA) in hyperglycemic rats. This is an experimental design with a post-test-only group design. Adult male Wistar rats were divided into three groups (n = 8): negative control (K-), positive control (K+), and treatment (P). The normality test was conducted using the Shapiro-Wilk test, followed by Kruskal Wallis. Results The olive oil was proven to reduce levels of Malondialdehyde in the P group, with an average of 2.29 nmol/mL lower than the K+ group. Although the olive oil reduced the average MDA level in hyperglycemic rats, the post-hoc test showed no difference in MDA between the K+ and P groups (p-value = 0.226). Conclusions Antioxidants in olive oil may effectively lower blood glucose levels, reducing levels of Malondialdehyde in hyperglycemic rats.
Effects of Stevia Leaf Extract on Blood Glucose and SOD Activity in Hyperglycemic Rats Zoni, Salsa Billa Pratami; Yerizel, Eti; Rahmatini
Jurnal Kesehatan - STIKes Prima Nusantara Vol 17 No 1 (2026): Jurnal Kesehatan Volume 17 Nomor 1 Tahun 2026
Publisher : LPPM Universitas Prima Nusantara Bukittinggi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35730/jk.v17i1.1603

Abstract

Background: Hyperglycemia is characterized by elevated blood glucose levels and may induce oxidative stress through excessive production of reactive oxygen species (ROS) that exceed the capacity of antioxidant defense systems. Stevia rebaudiana leaf extract (Stevia rebaudiana) contains antihyperglycemic and antioxidant compounds, including polyphenols and steviol glycosides. Objectives: This study aimed to determine the effect of Stevia leaf extract on blood glucose levels and Superoxide Dismutase (SOD) enzyme activity in hyperglycemic rats (Rattus norvegicus). Methods: This experimental study involved 35 rats divided into a negative control group (K−), a positive control group (K+), and three treatment groups (P1, P2, P3) receiving Stevia rebaudiana extract at doses of 100, 200, and 400 mg/kg body weight. Hyperglycemia was induced using alloxan. Blood glucose levels were measured using a digital analyzer, while SOD activity was assessed colorimetrically. Data were analyzed using the Kruskal–Wallis test followed by the Mann–Whitney test. Results: The mean blood glucose levels in groups K−, K+, P1, P2, and P3 were 93.2 mg/dL, 371.08 mg/dL, 134.3 mg/dL, 130.42 mg/dL, and 113.22 mg/dL, respectively. The mean SOD enzyme activity in groups K−, K+, P1, P2, and P3 were 14.92 U/mL, 13.53 U/mL, 14.57 U/mL, 14.74 U/mL, and 14.93 U/mL, respectively. The P3 group, which received a dose of 400 mg/kgBW, demonstrated the greatest effect on reducing blood glucose levels and increasing SOD activity. Conclusion: Stevia rebaudiana leaf extract significantly reduced blood glucose levels in hyperglycemic rats and showed a tendency to increase superoxide dismutase activity, although the differences in SOD activity were not statistically significant
Effects of Radiation on the Histology of the Tongue and Lip Mucosa in Rats : Preliminary Research Sagita Yudha; Aisyah Elliyanti; Eti Yerizel; Sukri Rahman
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 27 No. 03 (2026): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464) In Progress
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/eksakta/vol27-iss03/696

Abstract

The tongue and lip mucosa, as tissues with high cell proliferation rates, are highly susceptible to injury caused by ionizing radiation. This study aimed to evaluate histological changes in the tongue and lip mucosa of rats after exposure to ionizing radiation at various doses. Nine rats were used, each consisting of three rats, divided into a negative control group (K) and three treatment groups receiving radiation doses of 13 Gy (30 seconds of radiation), 15 Gy (66 seconds of radiation), and 17 Gy (110 seconds of radiation). Samples of tongue and lip mucosa tissue were harvested after 7 days and processed for histological examination using Hematoxylin and Eosin (HE) staining at 100x objective magnification. The results of this preliminary study showed that radiation at 13 Gy caused epithelial erosion, loss of filiform papillae, subepithelial bullae formation, and acute inflammatory cell infiltration on the tongue. At 15 Gy, more extensive ulceration with subepithelial edema was observed. At 17 Gy, total epithelial loss with massive subepithelial edema and vascular dilatation occurs. This preliminary study concludes that the tongue mucosa exhibits greater radiation sensitivity than the lip mucosa, with a dose-dependent progression of damage ranging from erosion to total epithelial loss.  
Effects of Radiation on the Histology of the Tongue and Lip Mucosa in Rats : Preliminary Research Sagita Yudha; Aisyah Elliyanti; Eti Yerizel; Sukri Rahman
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 27 No. 03 (2026): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/eksakta/vol27-iss03/696

Abstract

The tongue and lip mucosa, as tissues with high cell proliferation rates, are highly susceptible to injury caused by ionizing radiation. This study aimed to evaluate histological changes in the tongue and lip mucosa of rats after exposure to ionizing radiation at various doses. Nine rats were used, each consisting of three rats, divided into a negative control group (K) and three treatment groups receiving radiation doses of 13 Gy (30 seconds of radiation), 15 Gy (66 seconds of radiation), and 17 Gy (110 seconds of radiation). Samples of tongue and lip mucosa tissue were harvested after 7 days and processed for histological examination using Hematoxylin and Eosin (HE) staining at 100x objective magnification. The results of this preliminary study showed that radiation at 13 Gy caused epithelial erosion, loss of filiform papillae, subepithelial bullae formation, and acute inflammatory cell infiltration on the tongue. At 15 Gy, more extensive ulceration with subepithelial edema was observed. At 17 Gy, total epithelial loss with massive subepithelial edema and vascular dilatation occurs. This preliminary study concludes that the tongue mucosa exhibits greater radiation sensitivity than the lip mucosa, with a dose-dependent progression of damage ranging from erosion to total epithelial loss.  
IDENTIFIKASI MASALAH DAN PENYULUHAN KESEHATAN PADA LANJUT USIA DI NAGARI SUMANIAK Aisyah Elliyanti; Dian Pertiwi; Arina Widya Murni; Zelly Dia Rofinda; Yulistini Yulistini; Afriwardi Afriwardi; Eti Yerizel; Siti Nurhajjah; Elmatris Sy; Elizabeth Bahar
Jurnal Hilirisasi IPTEKS Vol. 2 No. 1 (2019)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jhi.v2i1.354

Abstract

Pelayanan kesehatan terhadap lanjut usia (lansia) perlu mendapatkan perhatian khusus, karena lansia rentan terhadap penyakit Tujuan: Pengabdian masyarakat ini dilakukan dengan tujuan untuk mengidentifikasi faktor risiko penyakit dan memberikan penyuluhan pada kelompok lanjut usia di Kenagarian Sumanik. Metode: Kegiatan yang dilakukan adalah pemeriksaan kesehatan lanjut usia dan pengkajian psikologis dengan menggunakan kuisioner DASS 42. Selanjutnya dilakukan penyuluhan kesehatan dengan harapan meningkatkan pengetahuan para lansia sehingga mampu mengidentifikasi tanda bahaya dan mendapat pertolongan lebih awal. Hasil: Kegiatan dihadiri oleh 63 orang warga, 31 orang diantaranya di atas usia 60 tahun. Hasil pemeriksaan ditemukan 22 orang mengalami hipertensi stadium 2, dan 5 orang menderita diabetes yang sebelumnya sudah diketahui akan tetapi kontrol tidak teratur. Pada pemeriksaan mata terdapat 25 orang menderita gangguan refraksi, 5 orang ekstensi lensa dan dirujuk ke RSUD melalui Puskesmas. Dari kajian psikologis didapatkan 5 orang memiliki gejala depresi, 15 orang dengan ansietas dan 48 orang mengalami stress-psikologis. Kesimpulan: Penyuluhan kesehatan sangat diperlukan terutama kelompok lanjut usia supaya lansia dapat mengenal tanda-tanda penyakit sehingga dapat pelayanan kesehatan lebih dini. Aktivitas fisik seperti berolahraga diperlukan namun lansia perlu mengetahui jenis olah raga yang tepat sesuai dengan usia.
The Effect of A High-Fat Diet on Lipid Profile Levels (LDL Cholesterol, HDL Cholesterol, Triglycerides, and Total Cholesterol) in Male Rattus Novergicus with Obese Ibrahim Ibrahim; Eti Yerizel; Endrinaldi Endrinaldi; Gusti Revilla
Indonesian Journal of Global Health Research Vol. 7 No. 6 (2025): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v7i6.164

Abstract

Obesity is a complex disease that has a significant impact on public health and its prevalence is increasing worldwide. Obesity causes lipid accumulation in adipose tissue. The purpose of this study was to determine the effect of a high-fat diet on lipid profiles in male white rats. This study used a true experimental study on male white rats with a post-test control-only group design. The group division in this study was divided into two groups, consisting of a negative control group (K1) of 5 rats and a HFD group (K2) of 20 rats. This study was conducted from October 2024 to April 2025 at the Biomedical and Biochemistry Laboratory, Faculty of Medicine, Andalas University. The population in this study were male white rats of the Rattus norvegicus strain wistar obtained from the Biomedical Laboratory, Faculty of Medicine, Andalas University. The sampling technique in this study used purposive sampling. The number of samples used in this study was 25 rats with a minimum reserve of 10%. The results of the study with the average values of LDL cholesterol, HDL cholesterol, Total cholesterol, and TG levels in the K1 group were 45.72, 62.28, 67.66, and 107.60 mg/dL, respectively, while in the K2 group they were 61.62, 43.14, 83.17, and 167.20 mg/dL, respectively. Based on the results of the independent T test, the significance of the two groups was 0.001, 0.001, 0.001, and 0.005, respectively, which means there was a significant or real difference in the average levels of LDL, HDL, total cholesterol, and triglycerides in the K1 and K2 groups.
Effect of probiotic-fermented milk containing Lactiplantibacillus pentoses strain HBUAS 53657 on serum glutathione peroxidase activity and pancreatic histopathology in hyperglycemic rats Rita Yaswir; Eti Yerizel; Netti Suharti; Cimi Ilmiawati
Acta Biochimica Indonesiana Vol. 7 No. 2 (2024): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.174

Abstract

Background: Hyperglycemia is characterized by elevated blood glucose levels caused by impaired insulin production or function, leading to oxidative stress. Probiotic-fermented milk containing Lactiplantibacillus pentosus strain HBUAS 53657 (PFM) has antioxidant and antimicrobial properties that may reduce oxidative stress. Objective: This study aimed to investigate the effects of PFM L. pentosus strain HBUAS 53657 on serum glutathione peroxidase (GPx) activity and pancreatic histopathology in hyperglycemic rats. Method: A post-test-only experimental design was conducted using 25 adult male Wistar rats divided into five groups (n = 5 per group): normal control, hyperglycemic control group, and hyperglycemic rats treated with PFM at doses of 1 × 10⁸ CFU/day (P1), 1 × 10⁹ CFU/day (P2), and 1 × 10¹⁰ CFU/day (P3) for 28 days. Serum GPx activity was assessed via spectrophotometry, Langerhans islet area was measured using ImageJ, and islet damage was evaluated using the Ningrum Score. Statistical analysis was performed using One-Way ANOVA and Kruskal-Wallis tests. Results: PFM administration at all doses significantly increased serum GPx activity (p = 0.002), with an optimal dose of 1 × 10⁹ CFU/day (P2). PFM also increased the Langerhans islet area (p = 0.001) and reduced islet damage scores (p < 0.001) in a dose-dependent manner. Conclusion: Probiotic-fermented milk containing Lactiplantibacillus pentosus strain HBUAS 53657 increases serum GPx activity, expands Langerhans islet area, and reduces islet damage in hyperglycemic rats.
Dose-dependent effects of Stevia rebaudiana leaf extract on malondialdehyde and catalase activity in alloxan-induced hyperglycemic rats Zhira Shada Athaya; Eti Yerizel; Dinda Aprilia; Rozi Abdullah; Fadrian; Westi Permata Wati; Husna Yetti
Acta Biochimica Indonesiana Vol. 9 No. 1 (2026): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.232

Abstract

Background: Diabetes mellitus-induced hyperglycemia triggers oxidative stress, characterized by elevated malondialdehyde (MDA) and impaired catalase (CAT) activity. Stevia rebaudiana, rich in steviol glycosides and polyphenols, demonstrates promising antioxidant properties, yet systematic dose-response data on oxidative stress biomarkers remain limited. Objectives: To evaluate the dose-dependent effects of stevia leaf extract on serum MDA levels and CAT activity in alloxan-induced hyperglycemic rats. Methods: Twenty-five male Wistar rats were allocated into normal control, diabetic control (alloxan 120 mg/kg), and three treatment groups receiving alloxan plus stevia extract at 100, 200, or 400 mg/kg body weight orally for 14 days (n=5/group). Serum MDA and CAT were measured spectrophotometrically. Results: Diabetic control showed significantly elevated MDA (2.68±0.62 mg/dL) versus normal control (1.78±0.30 mg/dL). Stevia extract dose-dependently reduced MDA: 1.70±0.19, 1.54±0.20, and 1.38±0.09 mg/dL at 100, 200, and 400 mg/kg, respectively, representing 36.6%, 42.5%, and 48.5% reduction. The 400 mg/kg dose achieved MDA levels comparable to normal control. CAT activity showed dose-dependent restoration trend (7.92±0.76 to 8.58±0.52 mg/dL). Conclusion: Stevia leaf extract (400 mg/kg BW) effectively reduces oxidative stress in hyperglycemic rats through significant dose-dependent MDA reduction, with potential catalase benefits requiring further investigation.
Co-Authors Ade Asyari Afriwardi Afriwardi Aga Pratama Ahmad Syukri Harahap Aisyah Elliyanti Al Hafiz Al Hafiz Al Ichram Novialdi, Rayhan Aljassri, Resti Karunia Amelin, Fitrisia Andani Eka Putra Angga Putra Perdana Anjelina Puspita Sari Ariani Zaltin Okvenda Arina Widya Murni Arni Amir Ashrifurrahman, Ashrifurrahman Asti Marian Sari Aumas Pabuti Bramadi Arya Caesar Rayhand Arrafif Nasution Cherlina Cherlina Daan Khambri Debby Apri Grecwin Deddy Saputra Desmawati Desmawati Dessy Arisanty Desy Arisanti Desy Arisanti Dezi Ilham Dia Rofinda, Zelly Dia Dian Pertiwi Dian Pertiwi Dina Arfiani Rusjdi Dinda Aprilia Dyka Aidina Ebill Fuji Edison Effy Huriyati Efrida Efrida Ega Purnamasari Eka Agustia Rini Eka Agustria Rini Eka Nofita Eliza Anas Elizabeth Bahar Elizabeth Bahar Elmatris Sy Endah Puji Septisetyani, Endah Puji Endrinaldi Eva Chundrayetti Eva Decroli F, Annisa Maida Fadil Oenzil Fadrian Fadrian, Fadrian Fauziah, Dita Firdawati Firdawati Fitri Zahara Fitriyana Fitriyana Gusti Revilla Hafni Bachtiar Hafni Bachtiar Hafni Bachtiar Hirowati Ali Hudila Rifa Karmia Husna Yetti Husnil Kadri Ibrahim Ibrahim Ilmiawati, Ilmiawati Irwan Ismawati Ismawati Jamsari Jamsari Janra, Muhammad Nazri Karmia, Hudila Rifa Karolin Trisnawelda Kastian, Ria Fajarwati Lili Irawati Maliza, Rita Masrul Djalal Masrul Syafri Meilisa Rahmawati Meiza Nerawati Mizwar Mizwar Mohamad Reza Muhamad Gerry Fadilla Muhammad Idris Munsyi Nares Wari Nadhifah Salsabila Nadila Andam Astari Netti Meilani Simanjuntak Netti Suharti Niki Astria Nila Kasuma Nila Kusuma Nita Afriani Nofrita Nofrita Novialdi . Nurhajjah, Sitti Nursal Asbiran Okvenda, Ariani Zaltin Prambudi Rukmono Pratama Elisa, Tasya Putri Pratita Jati Permatasari Putra Santoso Putri, Athifa Alya Rahma Putri Idaman Rahmadian, Rizki Rahmatina B Herman Rahmatini . Rahmawati, Meilisa Rauza Sukma Rita Raveinal Rayhan Al Ichram Novialdi Refi Amalia Utami Reny Jayusfani Revivo Rinda Pratama Rita Yaswir Rizki Rahmadian Ronaldi Noor Rozi Abdullah Rudy Afriant Sabar Hutabarat Sagita Yudha Sagita Yudha Saptino Miro Saptino Miro, Saptino Sari, Asti Marian Silvy Dioni Simanjuntak, Netti Meilani Sinurat, Arthauly Yopita Siti Nurhajjah Sukri Rahman Syahid MP, Miskah Indah Syahrul, Muhammad Zulfadli Tiara Dwi Pratiwi Tofrizal Tofrizal, Alimuddin Utami, Refi Amalia Vella Paraditha Wati, Westi Permata Wilson Novarino Yanwirasti Yanwirasti Yerizel, Almurdi Yerizel, Raveinal Yesi Mustika Sari Yulia Iriani, Yulia Yulistini, Yulistini Yusrawati Yusrawati Yusri Dianne Jurnalis Yustini Alioes Zelly Dia Rofinda Zhira Shada Athaya Zoni, Salsa Billa Pratami Zulkarnain Edward ZURAIDA ZURAIDA