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All Journal e-GIGI Jurnal Kedokteran Brawijaya Indonesian Journal of Cancer Chemoprevention Jurnal Kesehatan Andalas JIMKesmas (Jurnal Ilmiah Mahasiswa Kesehatan Masyarakat) The Indonesian Biomedical Journal Sari Pediatri Paediatrica Indonesiana Jurnal Gizi Klinik Indonesia Jurnal Kesehatan Medika Saintika B-Dent, Jurnal Kedokteran Gigi Universitas Baiturrahmah Acta Biochimica Indonesiana Jurnal Hilirisasi IPTEKS (JHI) Buletin Ilmiah Nagari Membangun Oto Rhino Laryngologica Indonesiana Majalah Kedokteran Andalas Jurnal Biomedika dan Kesehatan Syntax Idea Jurnal Pengabdian kepada Masyarakat Nusantara Jurnal Ilmu Kesehatan Indonesia (JIKSI) Jurnal Riset Rumpun Ilmu Kedokteran (JURRIKE) Jurnal Otorinolaringologi Kepala dan Leher Indonesia (JOKLI) Vitalitas Medis : Jurnal Kesehatan Dan Kedokteran Jurnal Sains, Teknologi dan Kesehatan
Eti Yerizel
Fakultas Kedokteran Universitas Andalas
Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Determinasi Jumlah Bakteri Porphyromonas Gingivalis ATCC 33277 pada Saliva Anak Stunting Nadhifah Salsabila; Nila Kasuma; Eti Yerizel
e-GiGi Vol. 12 No. 1 (2024): e-GiGi
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/eg.v12i1.47864

Abstract

Abstract: Nutrition affects the immune system and the development of oral health, including periodontal tissue health. Stunted children experience decreased salivary flow rate causing the growth of microorganisms that cause periodontal disease. Porphyromonas gingivalis is referred to one of the keystone pathogens in the development of periodontal disease. This study aimed to identify the number of Porphyromonas gingivalis ATCC 33277 bacteria in stunted child saliva. This was a descriptive study with a cross sectional design. Prior to collection, subjects were instructed to rinse their mouth for 30 seconds using distilled water, saliva was collected with draining method, then 2 mL of saliva was taken, and DNA was isolated from Porphyromonas gingivalis ATCC 33277. DNA amplification and DNA calculation of Porphyromonas gingivalis ATCC 33277 were performed by using Real-time PCR. The results obtained 23 stunted children age 6-12 years as subjects. The average number of Porphyromonas gingivalis ATCC 33277 bacteria in stunting child saliva was 230.8 x 106 ± 320.1 x 106 CFU/ml. In conclusion, the number of Porphyromonas gingivalis ATCC 33277 is higher in stunted children saliva than in normal child saliva. Keywords: stunted children; Porphyromonas gingivalis ATCC 33277 bacteria; periodontal diseases   Abstrak: Gizi memengaruhi sistem pertahanan dan perkembangan kesehatan rongga mulut termasuk kesehatan jaringan periodontal. Anak stunting mengalami penurunan laju alir saliva yang menyebabkan pertumbuhan mikroorganisme penyebab penyakit periodontal, antara lain Porphyromonas gingivalis sebagai salah satu keystone pathogens dalam perkembangan penyakit periodontal. Penelitian ini bertujuan untuk menentukan jumlah bakteri Porphyromonas gingivalis ATCC 33277 pada saliva anak stunting. Jenis penelitian ialah deskriptif dengan desain potong lintang. Sebelum pengambilan saliva, subjek diinstruksikan berkumur selama 30 detik menggunakan akuades. Pengambilan saliva dengan metode draining selama 10 menit, saliva terkumpul sebanyak 2 mL, dan dilakukan isolasi DNA bakteri Porphyromonas gingivalis ATCC 33277. Amplifikasi DNA dan perhitungan jumlah DNA bakteri Porphyromonas gingivalis ATCC 33277 dilakukan menggunakan Real-time PCR. Hasil penelitian mendapatkan 23 anak stunting dengan usia 6-12 tahun.  Rerata jumlah bakteri Porphyromonas gingivalis ATCC 33277 pada saliva anak stunting 230,8x106±320,1x106 CFU/ml yang lebih tinggi daripada anak sehat yaitu 180,8x106±201,8x106 CFU/mL Simpulan penelitian ini ialah rerata jumlah bakteri Porphyromonas  gingivalis ATCC 33277 pada saliva anak stunting lebih tinggi daripada saliva anak  normal. Kata kunci: anak stunting; bakteri Porphyromonas gingivalis ATCC 33277; penyakit periodontal
Co-Authors Ade Asyari Afriwardi Afriwardi Aga Pratama Ahmad Syukri Harahap Aisyah Elliyanti Al Hafiz Al Hafiz Al Ichram Novialdi, Rayhan Aljassri, Resti Karunia Amelin, Fitrisia Andani Eka Putra Angga Putra Perdana Anjelina Puspita Sari Ariani Zaltin Okvenda Arina Widya Murni Arni Amir Ashrifurrahman, Ashrifurrahman Asti Marian Sari Aumas Pabuti Bramadi Arya Caesar Rayhand Arrafif Nasution Cimi Ilmiawati, Cimi Daan Khambri Debby Apri Grecwin Deddy Saputra Desmawati Desmawati Dessy Arisanty Desy Arisanti Desy Arisanti Dezi Ilham Dia Rofinda, Zelly Dia Dian Pertiwi Dian Pertiwi Dina Arfiani Rusjdi Dyka Aidina Ebill Fuji Edison Effy Huriyati Efrida Efrida Ega Purnamasari Eka Agustia Rini Eka Agustria Rini Eliza Anas Elizabeth Bahar Elmatris Sy Endah Puji Septisetyani, Endah Puji Eva Chundrayetti Eva Decroli F, Annisa Maida Fadil Oenzil Fauziah, Dita Firdawati, Firdawati Fitri Zahara Fitriyana Fitriyana Gusti Revilla Hafni Bachtiar Hafni Bachtiar Hafni Bachtiar Hirowati Ali Hudila Rifa Karmia Husnil Kadri Irwan Ismawati Ismawati Janra, Muhammad Nazri Karmia, Hudila Rifa Karolin Trisnawelda Kastian, Ria Fajarwati Lili Irawati Maliza, Rita Masrul Masrul Syafri Meilisa Rahmawati Meiza Nerawati Mizwar Mizwar Muhamad Gerry Fadilla Muhammad Idris Nadhifah Salsabila Nadila Andam Astari Netti Meilani Simanjuntak Netti Suharti, Netti Niki Astria Nila Kasuma Nila Kusuma Nita Afriani Nofrita Nofrita Novialdi . Nurhajjah, Sitti Nursal Asbiran Okvenda, Ariani Zaltin Pratama Elisa, Tasya Putri Pratita Jati Permatasari Putra Santoso Putri, Athifa Alya Rahma Putri Idaman Rahmadian, Rizki Rahmatina B Herman Rahmatini . Rahmawati, Meilisa Rauza Sukma Rita Raveinal Rayhan Al Ichram Novialdi Reny Jayusfani Revivo Rinda Pratama Rizki Rahmadian Ronaldi Noor Rudy Afriant Sabar Hutabarat Saptino Miro Saptino Miro, Saptino Sari, Asti Marian Silvy Dioni Simanjuntak, Netti Meilani Sinurat, Arthauly Yopita Siti Nurhajjah Sukri Rahman Syahid MP, Miskah Indah Tiara Dwi Pratiwi Tofrizal Tofrizal, Alimuddin Utami, Refi Amalia Vella Paraditha Wilson Novarino Yanwirasti Yanwirasti Yaswir, Rita Yerizel, Almurdi Yerizel, Raveinal Yesi Mustika Sari Yulia Iriani, Yulia Yulistini, Yulistini Yusrawati Yusrawati Yusri Dianne Jurnalis Yustini Alioes Zelly Dia Rofinda Zulfadli Syahrul, Muhammad Zulkarnain Edward ZURAIDA ZURAIDA