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Synthesis and virtual screening of bis-(4-(tert-butyl)-N-(methylcarbamothioyl) benzamide)-Iron (III) complex as an anticancer candidate Ruswanto Ruswanto; Winda Trisna Wulandari; Richa Mardianingrum; Indah Cantika
Pharmaciana Vol 11, No 1 (2021): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (613.942 KB) | DOI: 10.12928/pharmaciana.v11i1.17837

Abstract

Thiourea derivatives were much used in drug discovery and drug-making, such as for an anticancer. The formation of drug complexes can increase lipophilicity through chelation formation, and the drug action is significantly upward due to the effective permeability to the center. In another study, the alteration of the compound becomes the complex with metal will grow in its activity so recently we have synthesized the Bis-(4-(Tert-Butyl)-N-(Methylcarbamothioyl) Benzamide)-Iron (III) complex.  The synthesis of Fe (III) metal with the 4-(Tert-Butyl)-N-(Methylcarbamothioyl) Benzamide in ethanol by reflux at 75oC for 7 hours. Hot Stage Microscopy, UV-Visible Spectrophotometry Infrared Spectrophotometry, and Massa Spectrophotometry were used to characterize the complex. This study concerns representing, inferring, and predicting pharmacokinetics and toxicity and molecular docking complexes. The complex weight was 0.29469 g. Its purity has been tested using the melting point determination and has obtained its range was 113o-115oC. The Characteristics of Bis-(4-(Tert-Butyl)-N-(Methylcarbamothioyl) Benzamide)-Iron(III) complex have a maximum wavelength of 260,0 nm and provide absorption of Fe-O vibrations at wavenumbers 478,2 cm-1and 588 cm-1, and the m/z complex of spectrophotometry mass was 559,31. The molecular docking process was performed using AutodockTools-1.5.6 software. It showed that Bis-(4-(Tert-Butyl)-N-(Methylcarbamo-thioyl)Benzamide)-Iron(III) complex could interact with ribonucleotide reductase enzyme, and it has better interaction than the 4-(Tert-Butyl)-N-(Methylcarbamothioyl)Benzamide with the binding affinity energy (ΔG)of  -8,52 kcal/mole and the constant inhibition (Ki ) of 568,55 nM.
Synthesis, Characterization and In Silico Study of Fe(III) Complex with N'-(4-Chlorobenzoyl)-Isonicotino-Hydrazide as Anti Tuberculosis Candidate Ruswanto Ruswanto; Fajar Setiawan; Nur Rahayuningsih; Richa Mardianingrum; Nur Laili Dwi Hidayati; Elsi Eryanti
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 6, No. 1, May 2020
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1586.366 KB) | DOI: 10.15408/jkv.v6i1.11788

Abstract

The synthesis of Fe(III) complexes with ligands N'-(4-Chlorobenzoyl)isonicotinohydrazide can be synthesized through mixing metal and ligand dissolved in ethanol by reflux at ± 75oC for 5 hours. The instruments for the characterization of the complex were used UV-Visible and Infrared Spectrophotometry. The aims of the study are: to determine the synthesis method, characterize of the complex, and to study the interaction of the complex with target receptors. The weight of the synthesized compound was obtained by 38.1 mg. The purity of the complex has been tested using the determination of melting point and got a melting point range was 196-198oC. The maximum wavelength of Fe(III)N'-(4-Chlorobenzoyl)isonicotinohydrazid) complex was  261.0 nm and provide absorption of Fe-O vibrations at wavenumbers 530.42 cm-1. The docking process was done using AutodockTools-1.5.6 software which shows that the Fe(III)N'-(4-Chlorobenzoyl) isonicotinohydrazide complex can interact with Enoyl-Acyl Carrier Protein Reductase from Mycobacterium Tuberculosis and it has better  interaction than isoniazid or N'-(4-Chlorobenzoyl)isonicotinohydrazide compound with the acquisition of free energy binding (ΔG) -9.80 kcal/mol and inhibition constant (Ki ) 0.06529 μM.
PENERAPAN 6M DAN PENTINGNYA CUCI TANGAN YANG BAIK DAN BENAR SEBAGAI UPAYA PENCEGAHAN PENULARAN COVID-19 Ruswanto Ruswanto; Vera Nurviana2 Nurviana; Dini Febianeu; Mitha Anggitha; Deliani Deliani; Alifia Nurfadhilah S; Gina Yulias Triyani; Tira Mutiara Utami; Wildan Rizki Asilmi; Rivaldi Muhsin
JMM (Jurnal Masyarakat Mandiri) Vol 5, No 5 (2021): Oktober
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (607.546 KB) | DOI: 10.31764/jmm.v5i5.5275

Abstract

Abstrak: Penyebaran Covid-19 di Indonesia sampai saat ini masih terbilang cukup tinggi dengan penambahan kasus positif semakin bertambah. Setiap hari kasus di Indonesia bisa mencapai 94 kasus dan ada 6.594 orang yang meninggal akibat virus ini sejak 21 Agustus 2020. Hal ini disebabkan karena rendahnya kemampuan literasi masyarakat, sehingga pemahaman atas merebaknya wabah Covid-19 tidak dapat dipahami secara maksimal. Tujuan kegiatan ini yaitu untuk memberikan informasi kepada masyarakat mengenai penerapan 6M serta cara mencuci tangan yang baik dan benar sehingga masyarakat dapat menerapkan ilmunya dalam kehidupan sehari-hari dalam upaya pencegahan wabah covid-19. Media yang digunakan yaitu brosur serta pemaparan materi tentang penerapan 6M dan pentingnya cuci tangan yang baik dan benar pada masa pandemic Covid-19. Kegiatan dinilai memiliki dampak yang positif berdasarkan evaluasi jangka pendek terdapat peningkatan pengetahuan dan kemampuan masyarakat Desa Panyiaran Kec. Cikalong mengenai penerapan 6M yang baik dan benar sekitar 71%.Abstract: The spread of Covid-19 in Indonesia is still quite high with the addition of positive cases increasing. Every day cases in Indonesia can reach 94 cases and there have been 6,594 people who have died from this virus since August 21, 2020. This is due to the low literacy skills of the community, so that understanding of the spread of the Covid-19 outbreak cannot be understood optimally. The purpose of holding this activity is to provide information to the public regarding the application of 6M and how to wash hands properly and correctly so that people can apply their knowledge in daily life in an effort to prevent the covid-19 outbreak. The media used are brochures and presentation of material about the implementation of 6M and the importance of good and correct hand washing during the Covid-19 pandemic. The activity is considered to have a positive impact based on a short-term evaluation there is an increase in the knowledge and ability of the people of Panyiaran Village, Cikalong District regarding the good and correct implementation of 6M about 71%.
SINTESIS DAN IDENTIFIKASI STRUKTUR SENYAWA 3-(3,5-DINITROBENZOIL)-1-FENILTIOUREA SEBAGAI KANDIDAT ANTIKANKER Susanti Susanti; Ruswanto Ruswanto; Muharam Priatna
Pharmacoscript Vol. 1 No. 2 (2018): Pharmacoscript
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat, Universitas Perjuangan Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36423/pharmacoscript.v1i2.102

Abstract

Thiourea derivatives are known to be potential anticancer agents. 3-(3,5-dinitrobenzoyl)-1-phenylthiourea as a thiourea derivatives has been synthesized through acylation between 3,5-dinitrobenzoyl chloride and 1-phenylthiourea in tetrahydrofuran with triethylamine ascatalyst through reflux process with stirring for 6 hours. The percentage of yield was13,86%. The purity of the product was shown by the single spot on the TLC and narrowrange of melting point. The structure identification results with UV spectrophotometry, IRspectroscopy and 1HNMR spectroscopy showed that the structure of the product wasappropriate to the prediction.
BIOAKTIVITAS DAN STUDI IN SILICO SENYAWA TURUNAN N’-BENZOYLISONICOTINOHYDRAZIDE (4-methyl, 4-chloro dan 3,5-dinitro) PADA Mycobacterium Tuberculosis (H37RV) BAKTERI GRAM POSITIF SERTA BAKTERI GRAM NEGATIF Ruswanto Ruswanto
Pharmacoscript Vol. 2 No. 2 (2019): Pharmacoscript
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat, Universitas Perjuangan Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36423/pharmacoscript.v2i2.239

Abstract

Isoniazid merupakan obat antituberkulosis yang memiliki aktivitas antimikobakterial yang baik yang bekerja secara aktif dengan menghambat biosintesis asam mikolat. Telah dilakukan pengujian In Vitro pada senyawa N’-(4-Methylbenzoyl) Isonicotinohydrazide N’-(4-Chlorobenzoyl) Isonicotinohydrazide dan N’-(3,5-Dinitrobenzoyl) Isonicotinohydrazide terhadap Mycobacterium tuberculosis H37Rv, bakteri gram positif serta bakteri gram negatif dengan menggunakan metode sumuran dan pada pengujian Mycobacterium tuberculosis H37Rv menggunakan metode REMA (Resazurin Microtiter Assay). Didapat nilai MIC terbaik pada senyawa N’-(3,5-Dinitrobenzoyl) Isonicotinohydrazide memiliki potensi tinggi sebagai antibakteri dengan nilai MIC 0,169 ppm terhadap bakteri e.colli. Ketiga senyawa yang diujikan pada  Mycobacterium tuberculosis H37Rv memiliki kemampuan sebagai anti tuberkulosis tetapi isoniazid lebih baik dari senyawa uji. Senyawa N’-(3,5-Dinitrobenzoyl) Isonicotinohydrazide di dockingkan pada reseptor 1KZN memiliki binding affinity yang lebih kecil dibandingkan senyawa pembanding yaitu isoniazid sebesar -6,89 kkal/mol.
HUBUNGAN KUANTITATIF STRUKTUR AKTIVITAS DAN DESAIN OBAT TURUNAN N'-BENZOYLISONICOTINOHYDRAZIDE SEBAGAI KANDIDAT ANTI TUBERKULOSIS Raja Ramadiansyah; Ruswanto Ruswanto
Pharmacoscript Vol. 3 No. 1 (2020): Pharmacoscript
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat, Universitas Perjuangan Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36423/pharmacoscript.v3i1.573

Abstract

Telah dilakukan analisa hubungan kuantitatif struktur aktivitas (HKSA) dan desain obat dari 12 senyawa turunan N’-benzoylisonicotinohydrazide terhadap Mycobacterium tuberculosis, Staphylococcus aureus, Bacillus subtilis, dan Escherichia coli berdasarkan pada perhitungan sifat kimia senyawa. Deskriptor yang digunakan dalam analisa HKSA diantaranya deskriptor hidrofobik, deskriptor elektronik dan deskriptor sterik. Data dekriptor diperoleh dari struktur hasil optimasi sedangkan data binding affinity diperoleh dari jurnal penelitian dan data MIC dari hasil pengujian secara in vitro. Analisa HKSA dilakukan dengan Multiple Linear Regression (MLR) menggunakan metode backward pada program SPSS. Hasil analisa HKSA menunjukkan bahwa terdapat hubungan kuantitatif struktur dan aktivitas dari 12 senyawa turunan N’-benzoylisonicotinohydrazide terhadap Mycobacterium tuberculosis, Staphylococcus aureus, Bacillus subtilis, dan Escherichia coli. Analisis hasil docking senyawa 3-{N'-[ (pyridine – 4 - yl) carbonyl] hydrazinecarbonyl} phenyl acetate dan senyawa 4-nitro-N'-[(pyridin-4-yl)carbonyl]benzohydrazide terhadap reseptor 2X23, senyawa N'-[(pyridin-4-yl)carbonyl]-4-(pyrrolidine-1 sulfonyl) benzohydrazide terhadap resptor 1JIJ, serta senyawa N'-[ (pyridine – 4 -yl) carbonyl] - 2,5 – bis (trifluoromethyl) benzohydrazide terhadap reseptor 1KZN menunjukkan bahwa semua senyawa usulan memiliki interaksi yang lebih baik terhadap masing-masingreseptor dibandingkan dengan INH karena senyawa-senyawa tersebut memiliki nilai binding affinity lebih kecil daripada INH. Hasil prediksi toksisitas menggunakan PreADMETmenunjukkan bahwa semua senyawa usulan menurut parameter ames test adalah mutagen.
Reverse Docking, Molecular Docking, Absorption, Distribution, and Toxicity Prediction of Artemisinin as an Anti-diabetic Candidate Ruswanto Ruswanto; Richa Mardianingrum; Siswandono Siswandono; Dini Kesuma
Molekul Vol 15, No 2 (2020)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (465.792 KB) | DOI: 10.20884/1.jm.2020.15.2.579

Abstract

Aldose reductase is an enzyme that catalyzes one of the steps in the sorbitol (polyol) pathway that is responsible for fructose formation from glucose. In diabetes, aldose reductase activity increases as the glucose concentration increases. The purpose of this research was to identify and develop the use of artemisinin as an anti-diabetic candidate through in silico studies, including reverse docking, receptor analysis, molecular docking, drug scan, absorption, and distributions and toxicity prediction of artemisinin. Based on the results, we conclude that artemisinin can be used as an anti-diabetic candidate through inhibition of aldose reductase
Uji In Vitro dan Studi In Silico Senyawa Turunan n’-benzoylisonicotinohydr Ruswanto Ruswanto; Nur Rahayuningsih; Nur Laeli Dwi Hidayati; Ginna Sri Nuryani; Richa Mardianingrum
JURNAL ILMU KEFARMASIAN INDONESIA Vol 17 No 2 (2019): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (704.686 KB) | DOI: 10.35814/jifi.v17i2.703

Abstract

There have been in silico and in vitro studies of N-Benzoylisonicotinohydrazide derivatives as antituberculosis candidates. The aims of this research were determined that the N'-benzoylisonicotinohydrazide derivatives could inhibit the activity of gram-positive, gram-negative and Mycobacterium tuberculosis, as well as having good interactions with Enoyl-Acyl Carrier Protein Reductase from Mycobacterium Tuberculosis. From the in vitro test it was found that the N'-benzoylisonicotinohydrazide compound had a Minimum Inhibitor Concentration (MIC) of 0.33 µg / ml against the Basillus subtilis, while the Minimum Inhibitor Concentration (MIC) of N '- (2-chlorobenzoyl) isonicotinohydrazide against Mycobacterium tuberculosis (H37Rv) was 3.125 µg / ml. From the in silico study, it was found that the binding affinity value between N '- (2-chlorobenzoyl) isonicotinohydrazide enzyme Enoyl-Acyl Carrier Protein Reductase (2X23) had the smallest binding affinity so that it could be predicted that it had a stable interaction than other compounds so that the N '- (2-chlorobenzoyl) isonicotinohydrazide could be used as a more potent antituberculosis candidate.
PENGETAHUAN DAN PEMAHAMAN MASYARAKAT DAERAH TASIKMALAYA TENTANG COVID-19 Ruswanto Ruswanto; Anna Yuliana; Firman Gustaman; Nurul Kamilah; Sukma Ayudia; Widya Oktaviani; Gina Maya Lestari; Oktaviani Ayu Saputri; Mina Fauziah; Fanisa Riadhiani; Paras Layna Safa; Yana Herdiana; Rika Zahara Dewi; Wiwin Kristiana; Melia Fujiyanti; Nida Puspa Dewi; Wini Wahyuni; Alicia Nadira; Mochamad Fajar Deliaz; Wemfi Riska Roswandi; Wina Aprilia Setiawati
Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 4, No 2 (2021): Martabe : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jpm.v4i2.538-544

Abstract

Saat ini Corona Virus menjadi perbincangan banyak negara di dunia termasuk Indonesia didalamnya. Virus ini sudah menjadi masalah diseluruh dunia karena dengan penyebaran yang begitu cepat dan mematikan. Penularannya hanya melalui kontak dengan penderita yang ditularkan begitu cepat. Virus ini membawa dampak yang signifikan terhadap kehidupan manusia dengan melemahnya ekonomi masyarakat. Tujuan dari kegiatan ini adalah untuk mengetahui tingkat pengetahuan dan pemahaman masyarakat terhadap  Covid-19. Metode yang digunakan dalam rangka untuk studi pendahuluan pengabdian masyarakat yaitu dengan menyebar kuesioner dan mengumpulkan data-data mengenai pengetahuan masyarakat dan cara penanggulangan Covid-19 secara sederhana di masyarakat. Hasil penelitian dapat disimpulkan bahwa kebanyakan masyarakat sudah mengetahui cara menanggulangi penyebaran virus ini secara sederhana. Untuk dapat mencegah penyebaran virus ini sangat diperlukan kerjasama oleh semua orang dengan sadar dan saling mengingatkan dalam upaya pencegahan penyebaran virus ini.
Penyuluhan New Normal Lifestyle With Herbs dan Pembagian Sediaan Herbal Untuk Kesehatan Ruswanto Ruswanto; Vera Nurviana; Lusi Nurdianti; Anindita Tri Kusuma Pratita; Annisa Pebiansyah; Taufik Hidayat
Jurnal Kreativitas Pengabdian Kepada Masyarakat (PKM) Vol 5, No 2 (2022): Volume 5 No 2 Februari 2022
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jkpm.v5i2.4728

Abstract

ABSTRAK Adanya peningkatan kasus covid-19 yang terjadi di seluruh belahan dunia maka perlu adanya langkah-langkah yang harus dilakukan sehingga dapat mengurangi tingkat penyebaran covid-19 yang makin tinggi. Tujuan pengabdian masyarakat yang dilaksanakan adalah untuk meningkatkan pemahaman kesehatan pemuda/pemudi dimasa new normal dengan pemanfaatan obat tradisional dan mengenalkan sediaan herbal untuk kesehatan. Metode yang dipakai adalah ceramah dah diskusi (penyuluhan) dan pembagian sediaan herbal yang bermanfaat untuk kesehatan di wilayah Tasikmalaya. Peserta kegiatan ini adalah muda/mudi yang tergabung dalam organisasi SIRAM Kota Tasikmalaya serta warga masyarakat di Tasikmalaya.  Dari kegiatan yang dilaksanakan dapat dirasakan adanya peningkatan pengetahuan tentang new normal dan sediaan herbal oleh peserta sekitar 75%. Keyword: covid-19, herbal, new normal, penyuluhan  ABSTRACT With an increase in COVID-19 cases occurring in all parts of the world, it is necessary to take steps to reduce the spread of Covid-19 which is getting higher. The community service purpose was (1) to increase the understanding of the health of young people in the new normal era by using traditional medicines and (2) introducing herbal preparations for health. The method used is lectures and discussions (counseling) and distribution of herbal preparations that are beneficial for health in the Tasikmalaya area. The participants of this activity are young people who are members of the SIRAM organization in the City of Tasikmalaya and residents of the community in Tasikmalaya. From the activities carried out, it can be felt that an increase in knowledge about the new normal and herbal preparations by the participants about 75%. Keywords: covid-19, herbs, new normal, socialization
Co-Authors A.A. Ketut Agung Cahyawan W Aas Nuraisah Aas Nuraisah Aas Nuraisah Abdul Hakim Agus Susanti Aguslina Kirtishanti Ahmad Tantowi Jaohari Ai Sarah Ai Teni Siti Robi`ah Aimi Ratnasari Aimi Ratnasari, Aimi Albie, Fadhlan Adtya Alicia Nadira Alifia Nurfadhilah S Anggi Agustira Anindita Tri Kusuma , Pratita Anindita Tri Kusuma Pratita Anindita Tri Kusuma Pratita Anindita Tri Kusuma Pratita Anindita Tri Kusuma Pratita Anindita Tri Kusuma Pratita Anis Nasipah Anisa Pebiansyah Anjani, Desti Lara Anjuni, Dhea Putri Anna Yuliana, Anna Annazalia Rustandi Putri Annisa Pebiansyah Annisa Pebiansyah Aprillia, Ade Yeni Arry Yanuar Asep Nugraha Ayudia, Sukma Billah, Tazkia Hasna Citra Dewi Salasanti Citra Dewi Salasanti Damayanti, Okky Deden Makbuloh Deliani Deliani Delis Susilawati Desri Lestari Dewi Dewi Dewi, Nida Puspa Dewi, Rika Zahara Diana Sri Zustika Dini Febianeu Ditha Rizqi Aulia Utami Dudi Nurmalik Dwijanto Dwijanto, Dwijanto Elsa Oktavia Angelica Elsi Eryanti Fadilah, Refi Tazhqiyatul FAJAR SETIAWAN Fajar Setiawan Fanisa Riadhiani Fathurohman, Mochamad Fauziah, Mina Febby, Pratama Feri Sandria Firiani, Yuli Firman Gustaman Fujiyanti, Melia G. Gunawan Garna, Imam Mustaqim Gatut Ari Wardani Gina Maya Lestari Gina Septiani Agustien Gina Yulias Triyani Ginna Sri Nuryani Gustaman, Firman Hasan Sastra Negara Hasanah, Melati Nur Aini Hendy Suhendy Herdiana, Yana Heri Herdiana Heru Juabdin Sada Hery Wibowo, Hery Himyatul Hidayah Himyatul, Hidayah Holis Abdul Holik, Holis Abdul Ibnu Hajar Ikram, Nur Kusaira Khairul Ilham Alifiar Ilham Alifiar Ilham Alifiar Ilham Nurfadilah, Asopari Indah Cantika Ira Rahmiyani Irawan, Rudy Isti Daruwati Isti'ana, Ais Isti’ana, Ais Kamiel Roesman Bachtiar Khoerunisa, Widia Salsabila Kirana Azzahra Emil, Musa Korry Novitriani, Korry Kristiana, Wiwin Leli Siti Zaqiah Lestari Wahdah Lestari, Gina Maya Lilis Tuslinah Lilis Tuslinah Lilis Tuslinah Lilis Tuslinah, Lilis Lina Rahmawati Rizkuloh Lusi Nurdianti, Lusi M. Faturohman Mardhiah Mardhiah Mardhiah Mardhiah Mardianingrum Richa Melia Fujiyanti Meylany Sity Rossy Lestary Mina Fauziah Mitha Anggitha Moch. Zainuddin, Moch. Mochamad Fajar Deliaz Mochamad Fathurahman Mochamad Fathurohman Mochamad Fathurohman Moh. Ismail Muhammad Ismail Muharam Priatna Muharam Priatna Muharam Priatna Nadira, Alicia Naser Fahmi Muhamad Nasipah, Anis Neni Sri Gunarti Neta Ekayanti Suganda Nida Puspa Dewi Nisa Uswatun Khasanah Nofianti, Tita Nofianti, Tita Nofriyaldi, Ali Nugraha, Asep Nugraha, Hafizh Maulana Nur Aji Nur Laeli Dwi Hidayati Nur Laili Dwi Hidayati Nur Rahayuningsih Nur Rahayuningsih Nur Rahayuningsih, Nur Nur, Rahayuningsih Nurlaili Dwi Hidayati Nurlita, Putri Nurmalik, Dudi Nurmalik, Dudi NURUL AZIZAH Nurul Kamilah Nurul Kamilah Oktaviani Ayu Saputri Oktaviani, Widya Paras Layna Safa Pebiansyah, Anisa Pertiwi, Nur Ihsani Pikri Adit Praditya R Pratama, Febby Pratita, Anindita Tri Kusuma Putri Pratiwi Putri Putri, Putri Rachman, Dineu Septy Ulfiatur Rahmawati Rahmawati Raja Ramadiansyah Ramadan, Fajar Renadi, Sedin Riadhiani, Fanisa Richa Mardianingrum Richa, Mardianingrum Rika Zahara Dewi Rissa Putri Aulia Yulianto Rivaldi Muhsin Roswandi, Wemfi Riska Saeful Amin Safa, Paras Layna Saputri, Oktaviani Ayu Sarah, Ai Sarwatiningsih, Yunia Sa’idy, Sa’idy Septian, Ade Dwi Setiawati, Wina Aprilia Shal Nurdinda Fauziah Silvi Novitasari Silvi Novitasari, Silvi Sindi Lestari Siswandono, Siswandono Sri Asih Sri Mulyani Srie Rezeki Nur Endah Srie Rezeki Nur Endah Sukma Ayudia SUPRIADI, HENY Surya Amal Surya, Amal Susanti Susanti Susanti Susanti Susanti Susanti SUSILAWATI , BETI Taufik Hidayat Tiara Permata Sari Tifa Nofianti Tira Mutiara Utami Tita Nofianti Tita Nofianti Tita Nofianti Tita Nofianti Tita Nofianti Tita Nofianti Tita Nofianti, Tita Tresna Lestari Tresna Lestari, Tresna Tuslinah, Lilis Tuslinah, Lilis Ummy, Mardiana Vera Nurviana Vera Nurviana Veronika Yulianti Susilo Wahdah, Lestari Wahyuni, Wini Wemfi Riska Roswandi Widya Oktaviani Wildan Rizki Asilmi Wina Aprilia Setiawati Winda Trisna Wulandari, Winda Trisna Wini Wahyuni Wiwin Kristiana Yana Herdiana Yeni Ari Safitri, Dalimunthe Yulia Salmini Yundari, Yundari YUSNITA, ERNI