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Identification of Pathogenic Bacteria from Striped Catfish (Pangasionodon hypophthalmus) kept in Aquaculture Ponds Jaya Maruli Tamba; Henni Syawal; Iesje Lukistyowati
Jurnal Perikanan dan Kelautan Vol. 26 No. 1 (2021): February
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.26.1.40-46

Abstract

Koto Masjid Village is the largest production center in striped catfish fish farming activities in Riau Province. One of the inhibiting factors is the disease caused by bacteria, this study aims to determine the types of pathogenic bacteria that have the potential to infect striped catfish. This research was conducted in August - November 2019, samples of striped catfish used in the study amounted to 15 tails with an average length of 15-25 cm taken from 5 ponds in Kampung II, Koto Masjid Village, XIII Koto Kampar Subdistrict, and then identified bacteria by Conventional Laboratory in Fish Parasites and Diseases, Faculty of Fisheries and Marine, Riau University, Pekanbaru. This study uses a survey method that is by taking samples from the field in Pu and then the isolation of bacteria is carried out from the kidney organ in the TSB media then purified on the TSA media. The results of the study found 3 types of pathogenic bacteria namely Aeromonas sp., Edwardsiella sp., and Pseudomonas sp. Water quality during the study was pH 6.6-70, DO 4.05-4.35 mg/L, NH3 0.035-0.2 mg/L and Temperature 28-300C.
Description of Erythrocytes of Striped Catfish (Pangasionodon hypophthalmus) Infected by Edwardsiella tarda Treated with Mango Leaf (Mangifera indica) Mariana Lumban Tungkup; Henni Syawal; Morina Riauwaty
Jurnal Perikanan dan Kelautan Vol. 26 No. 1 (2021): February
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.26.1.58-65

Abstract

This research has been carried out from November to December 2019 at the Laboratory of Parasites and Fish Diseases at the Fisheries and Marine Faculty of Riau University. The aim of the study was to obtain the best dose of mango leaf solution for the treatment of striped catfish (P. hypophthalmus) infected with E. tarda, seen from the erythrocyte picture. The method used was an experiment with a Completely Randomized Design (CRD) of a factor of five treatment levels and replications three times. Test fish used were striped catfish measuring 8-12 cm, with a total of 150 fish. The treatments used for treatment were 1000 ppm, 1200 ppm, 1400 ppm, negative controls (not infected and not treated), and positive controls (infected and not treated). Parameters measured and observed among others; total erythrocytes, hemoglobin levels, hematocrit values, erythrocyte cell morphology and survival rate. The results showed that apple mango leaf solution (M. indica) was able to treat conjoined striped catfish infected with E. tarda by Emmersion method. The best treatment of 1200 ppm is characterized by an average total erythrocyte, mean total erythrocytes 149.33 x 104 cells / mm3, hemoglobin level 9.03 g / dL, hematocrit value 35.67% and the survival of the test fish after treatment is 86.67%. The results of water quality measurements during the study, namely temperature 27.00-27.46 0C, pH 6.1-6.77, dissolved oxygen 3.53-3.87 mg/L, and ammonia 0.02 mg/L.
Effect of Curcumin in Feed for Treating Striped Catfish (Pangasiodon hypopthalmus) Infected Bacteria Aeromonas hydrophila Poltak Pakpahan; Henni Syawal; Morina Riauwaty
Jurnal Perikanan dan Kelautan Vol. 25 No. 3 (2020): October
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.25.3.224-231

Abstract

Curcumin is one of the secondary metabolite compounds products that can increase the immune system of fish against bacterial attack. The purpose of this research was to find out the changes in the erythrocytes of a striped fish that were infectious bacteria A. hydrophila and treated with feeding containing curcumin and know the best dose of curcumin extract in the feed. This research was conducted in September to November 2019. The test fish used was the seed of a striped fish that measures 8-10 cm by 150 tails. Method used was the method of experimental and complete random design a factor of 5 levels of treatment and 3 times repeated, the treatment was Kn (feeding without the extract of curcumin and not infected with bacteria A. hydrophila), Kp (feeding without being given curcumin extract and infectious bacteria (A. hydrophila), P1 (addition of curcumin in feed at a dose of 0.7 g/kg), P2 (0.9 g/kg), P3 (of 1.1g/kg). The results shown that P3 treatment was the best treatment with a < 0.05 P where in a total of erythrocytes was 2.46 x 106 cells/mm3. Hemoglobin 9.4 g/dl, hematocrit value of 32.3%, and survival rate was 90%. Curcumin was able to treat fish from an A. hydrophila infection
The Role of Vitamin C and Thyroxine Hormone (T4) on the Growth of Asian Redtail Catfish (Hemibagrus nemurus) in the Recirculation System Anang Widarsa; Usman M Tang; Henni Syawal
Jurnal Perikanan dan Kelautan Vol. 29 No. 1 (2024): February
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/138-144

Abstract

This research was conducted in January-July 2021 at the Marine Science Technology Biology Laboratory, Faculty of Fisheries and Marine Sciences, Universitas Riau. The purpose of the study was to see the effect of a combination dose of vitamin C and thyroxine hormone (T4) on the growth rate and survival of bream reared in a recirculation system. The method used was an experimental method using a factorial complete randomized design (CRD) with 2 factors and 3 replicates. The first factor is vitamin C, while the second factor is the dose of tyrrhosine hormone (T4) given during maintenance. The doses of vitamin C used in this study were; 0 mg/kg (V0), 500 mg/kg (V1), 1000 mg/kg (V2). Doses of thyroxine hormone (T4) used were doses; 0 mg/kg (H0), 4 mg/kg (H1), 6 mg/kg (H2), 8 mg/kg (H3). The Asian redtail catfish used were 8-10 cm in size and reared in a round container made of PE plastic with a diameter of 56 cm and a height of 48 cm, totaling 36 units. The pellets used were Hi-Provite 781-2 with 31% protein, with feeding done 3 times a day ad satation. The results showed that the addition of vitamin C and thyroxine hormone influenced the growth of Asian redtail catfish reared in the recirculation system. The combination of vitamin C 1000 mg/kg and thyroxine hormone 8 mg/kg feed gives the highest growth and survival, seen from the absolute weight growth rate of 12.97 g, specific growth rate of 1.93 g/day, and 95% survival. Water quality during rearing was favorable for the growth of Asian redtail catfish, with temperature ranging from 28-30oC, pH 6.5-7.0, and DO 4.2-5.95 ppm.
Description of Erythrocyte of Clarias gariepinus Infected by Aeromonas hydrophila and Treated with Bay Leaf Extract (Syzygium polyantha) Maya Cerlina; Morina Riauwaty; Henni Syawal
Jurnal Perikanan dan Kelautan Vol. 27 No. 1 (2022): February
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.27.1.105-113

Abstract

Bay leaf (Syzygium polyantha) has antibacterial compounds like flavonoid, tannin, alkaloid, essential oil, vitamin C, and minerals. The purpose of this study was to obtain the best dose of bay leaf solution to cure catfish infected by Aeromonas hydrophila and seen from total erythrocytes, hemoglobin levels, hematocrit levels, and morphology of erythrocytes. The method used is an experimental method with a Completely Randomized Design (CRD), one factor with five treatments. The treatment applied is Kn (without immersed with bay leaf solution and not infected by A.hydrophila), Kp (without immersed with bay leaf solution and infected by A.hydrophila), P1 (100 ppm), P2 (1100 ppm), P3 (1200 ppm). The curing is done by immersed with bay leaf solution for 5 minutes, carried out three times with an interval of 24 hours. The results show that bay leaf solution able to cure catfish infected by A.hydrophila. The best dose is 1200 ppm with total erythrocytes was 244 x 106 cell/mm3, hemoglobin level was 11,67 g/dL, hematocrit level was 34,33% and the survival rate was 96,67%. Water quality during the study is temperature 27,3-28,0°C, pH 6,5-6,8, DO 3,4-4,2 mg/L and ammonia 0,016-0,019 mg/L.
DESCRIPTION OF LEUKOCYT OF SIAM PATIN FISH (Pangasius hypophthalmus) THAT FEED BY ADDITION OF HARUMANIS MANGO SEEDS (Mangifera indica L.) Syaieba, Mutiara; Lukistyowati, Iesje; Syawal, Henni
Asian Journal of Aquatic Sciences Vol. 2 No. 3 (2019): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (738.829 KB)

Abstract

Harumanis mango seed (Mangifera indica L.) is a natural ingredient that can be used as an immunostimulant. The phytochemical content in harumanis mango seeds (M. indica L.) include tannins, flavonoids, saponins and ascorbic acid. This study aims to get the best dose of adding harumanis mango seed solution (M. indica L.) to the feed. The method used was an experimental method with a Completely Randomized Design (CRD) consisting of one factor with four treatment levels which included P0 without the addition of harumanis mango seed solution, P1 addition of harumanis mango seed solution at a dose of 1.6 mL/Kg of feed, P2 addition harumanis mango seed solution at a dose of 1.8 mL/Kg of feed and P3 addition of harumanis mango seed solution at a dose of 2 mL/Kg of feed. Test fish used were Siamese catfish (P. hypophthalmus) measuring 8-12 cm which were kept in cages for 60 days. The results showed that administration of harumanis mango seed solution to feed affected the image of leukocytes of Siamese catfish (P. hypophthalmus) (P < 0,05). The best dose is in the treatment of P3 (2 mL/Kg of feed) with an average total leukocytes 9.76 x 104 cells/mm3, leukocrit levels of 1.67%, lymphocytes 82.00%, neutrophils 7.67%, monocytes 10, 33%, phagocytic activity 31.67% and absolute weight growth of 37.70 g, and 95% survival.
EFFECT OF rGH HORMONE ADMINISTRATION ON PROTEASE ENZYME ACTIVITY AND RNA/DNA RATIO OF STRIPED CATFISH (Pangasianodon hypophthalmus) MAINTAINED IN SALINE MEDIA Syahputra, Mohamad Ardani; Tang, Usman M; Syawal, Henni
Asian Journal of Aquatic Sciences Vol. 7 No. 1 (2024): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Striped catfish is a prevalent fish and is in great demand. Striped catfish cultivation generally uses fresh water as a living medium, but several freshwater fish commodities are starting to be developed to adapt to saline media. Harvesting striped catfish usually takes approximately 3 - 8 months. To overcome this, one way is to use growth hormone. The growth hormone currently used is Recombinant Growth Hormone (rGH). GH administration has been reported to have various effects, particularly on protein metabolism. In fish digestion, protein from feed cannot be directly absorbed but is first broken down by protease enzymes into amino acids. The physical appearance, which is the phenotype of an organism, is the result of metabolic processes in each cell that makes up the organism. This research aimed to analyze the effect of administering the rGH hormone on protease enzyme activity, RNA/DNA ratio, and growth of striped catfish maintained in saline media. The method used was experimental in the form of a Completely Randomized Design (CRD) with four treatments and three replications. Based on the results obtained during the research, it shows that Protease Enzyme Activity (U/minute) and the best RNA/DNA Ratio use a dose of 3 mg/kg with respective values ​​of 0.75±0.24 U/minute and3.30±0.49.
THE USE OF Kaempferia galanga AND Curcuma xanthorrhiza FERMENTED TO IMPROVE THE IMMUNITY OF Oreochromis niloticus AGAINST Streptococcosis Khairia, Khairia; Syawal, Henni; Luksityowati, Iesje; Riauwaty, Morina
Asian Journal of Aquatic Sciences Vol. 6 No. 3 (2023): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Streptococcus agalactiae is a bacterium that causes streptococcosis, which attacks tilapia. This research was conducted from March to June 2022 at the Laboratory of Parasites and Fish Diseases, Faculty of Fisheries and Marine, University of Riau. This study aims to understand the best dose of fermented Kaempferia galanga and Curcuma xanthorrhiza, which are added to the feed to increase Oreochromis niloticus immunity. The method used was experimental with a completely randomized design (CRD) with five treatment levels and three replications. The treatments applied were Kn: Negative control (no treatment used), Kp: positive control (no treatment used and infected with S.agalactiae), P1 (100 mL/kg feed), P2 (125 mL/kg feed), P3 (100 mL/kg feed) and infected with S. agalactiae. The fish were contaminated on the 32nd day at a density of bacteria 108 CFU/mL of 0.1 mL/fish intramuscularly, and then the fish were reared up to the 46th day. The results showed that the fermented K.galanga and C.xanthorrhiza affected the haematology of O.niloticus. The best dose was in P3 with a dose of 150 ml/kg of feed which showed total erythrocytes of 2.01 x106 cells/mm3, hematocrit value of 27.33%, a hemoglobin level of 7.93 g/dL, total leukocytes of 6.59 x104 cells/mm3, lymphocytes 77.33%, monocytes 14.00%, neutrophils 8.67%, growth with a weight of 34.06 g, a length of 7.5 cm and a survival rate of 93.33%. Based on the data obtained, it can be concluded that fermented K.galanga and C.xanthorrhiza can increase the immunity of O.niloticus against Streptococcosis disease.
THE EFFECT OF SALINITY ON THE PHYSIOLOGICAL PERFORMANCE OF ASIAN REDTAIL CATFISH SEEDS (Hemibagrus nemurus) Putra, Mutlas Ade; Tang, Usman M; Syawal, Henni
Asian Journal of Aquatic Sciences Vol. 6 No. 3 (2023): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

This research was conducted 30 days from June to July 2022 at the Aquaculture Laboratory, Faculty of Fisheries and Marine Sciences, Universitas Riau, Pekanbaru. This study aimed to analyze the osmoregulation response of the Asian redtail catfish to different salinities and to obtain optimal salinity for good physiological performance for the Asian redtail catfish. The method used was an experimental method using a completely randomized design (CRD) with five treatments and four replications, namely, three ppt salinity treatment, six ppt salinity treatment, nine ppt salinity treatment, 12 ppt salinity treatment, and 15 ppt salinity treatment. The test fish used were Asian redtail catfish with an average size of 7.17±0.04 cm. Asian redtail catfish seeds were obtained from the Tibun Fish Seed Center, Kampar. The container used is an aquarium with a size of 60x40x40 cm. Research data shows that nine ppt salinity is the best for Asian redtail catfish osmoregulation of 0.5 mOsm/L H2O. The best cortisol hormone content is found at three ppt salinity, the lowest oxygen consumption is at three ppt salinity of 0.9 mgO2/g/hour, the best survival was found at three ppt salinity of 100%, and the growth in weight and length was in the three ppt treatment of 8.90 g and 2.04 cm. Water quality parameters include temperature 25-30oC and dissolved oxygen 5.04-8.04 ppm.
PROFILE ERYTHROCYTES OF Oreochromis niloticus FED WITH HERBS FERMENTED AND INFECTED WITH Aeromonas hydrophila Nurfitri, Maya; Syawal, Henni; Lukistyowati, Iesje; Riauwaty, Morina
Asian Journal of Aquatic Sciences Vol. 6 No. 3 (2023): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Herbs Fermented consist of Kaempferia galanga L, Curcuma xanthorrhiza Roxb, and Curcuma domestica Val, which are environmentally friendly disease prevention alternatives that can be added to feed because they have several advantages, such as increasing appetite, digestion, growth, disease resistance, and reducing stress levels in fish. This research was carried out from August to December 2021 at the Marine Science Biotechnology Laboratory and Fish Parasites and Diseases Laboratory, Faculty of Fisheries and Marine, Universitas Riau. This study aimed to obtain the best dose from adding fermented herbs to increase the immune system of Oreochromis niloticus and prevent diseases caused by Aeromonas hydrophila bacteria in terms of profile erythrocytes. The method used was an experimental method using a Completely Randomized Design (CRD) with one factor, five levels of treatment, and three replications so that 15 experimental units were obtained. The challenge test was carried out on the 32nd day using A.hydrophila with a density of 108 CFU/mL. The best dose was found in P3 with a dose of 200 mL/kg of feed, which showed a total erythrocyte of 2.09x106 cells/mm3, hemoglobin level of 7.70 g/dL, hematocrit value of 37.70%, and a normal erythrocyte morphology. Water quality ranged from temperature 27-29oC, pH 6.9-7.2, DO 3.1-4.4 mg/L, and Ammonia 0.016-0.024 mg/L. Based on the data obtained, it can be concluded that fermented herbs can increase the immunity of O. niloticus to diseases caused by A.hydrophila.
Co-Authors Abdul Alif Abdul Alif Addilah, M. Fikri Adelina &#039; Afra Azizah Afrina Putri Afrizal Tanjung Alfian &#039; Alfinda, Rudi Alif, Abdul Amraini Fitri Anang Widarsa Andarini Diharmi Anderson.s, Roi Andesra, Andesra Andi Almer Farrand Noor Andre A Saragih, Andre A Andre Manusun Purba Anggriani, Rara Dewi Angraika Dirta Annisa Dwi Putri Anugrah, Rio Persal Anzila Rizki W Muharram Asmika Harnalin Simarmata Athif Amirah Elza Atika Talitha Erman Atria Martina Azizah, Afra Cerlina, Maya Chandra Chandra Chania Arnata Christin Natalia Cici Adliana Darfia, Novreta Dea Switzelin H Destriman Laoli Devy P Lumban Gaol Dian Fitria M Dian Iriani Dian Puspitowati Dicky Azwar Lubis Dinar Mey Jasika H Dwi Indah Fransiska Elfira Stefani Priska Erikson Sahala Pardamean Etri Nopilita Faira Yovanie Fandi Rahmat Farkhan, Mochammad Farrand Noor, Andi Almer Fatihaturrahma, Annisya Fauzi Ahmad Lubis Faza, Farzana Fakhira Fazira, Meisya Amanda Febi Nabila Feri Julianto Fitria Sufriyani Fransiska, Dwi Indah Fricilia Zissalwa Gaol, Devy P Lumban Ginting, Kiki Dirgantara Gunawan B S Gusriansyah, Dimas H Herdini Hafil Kurnia Tama Hasbullah Zakky Hasugian, Sampang Hotramadi Herianti Rambe Heriyanti Heriyanti, Heriyanti Herparinda Novita Husna, Hidayatul Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukstyowati Iesje Lukystiowati Ilham Fajri Pohan Iman, Khasan Nur Ipa, Adha Irma Yunita Irsya, Habib Irwan Effendi Irwan Irwan Iskandar Putra Isma Mulyani Isna Sudirman Isna Sudirman Isnaini Lidya Rizki Jampil, Tirta Herlis Jan Stepfely Simanjuntak Jaya Maruli Tamba Juniarty Manurung Juniarty Manurung Juwita Lestari Karnila, Rahman Karnila Khairia, Khairia Kiky Dirgantara Ginting Kurniawan, Ahmad Juli Kusai, Kusai Latifah Hannum Lase Lestari, Rona Luksityowati, Iesje Luskityowati, Iesje M Riswan M Zaky Ditiya Putra M. Riswan M. Yanda, M. Yanda Manurung, Juniarty Mariana Lumban Tungkup Mariska, Refi Marwati &#039; Maulina Sawitri Maya Cerlina Maytra Wirdona Meisya Amanda Fazira Melin Siburian Memi Martin Ilmayati Mery Sukmiwati Mesi Susanti Mochammad Farkhan Morina Riauwaty Muhamad Latiful Khobir Muhammad Akram Muhammad Arga Saputra Muhammad Arga Saputra Muhammad Arga Saputra Muhammad Azaldin Muhammad Muhajir Muhammad Muhajir, Muhammad Mutawakkil, M. Mufid Al Mutiara Mutiara Nabellia Nabellia Nabellia, Nabellia Nabila, Febi Nasril Ismail NASTITI KUSUMORINI Nelli Karolina Sitorus Novike Zullanda Selvi, Novike Zullanda Novita, Herparinda Novreta Ersyi Darfia Nur Addini Nur Aini Nur Asiah Nuraini Nuraini Nuraini Nuraini Nuraini Nuraini Nurfitri, Maya Nurhasan Nurhasan Nurhasan Nurhasan, Nurhasan Oktavianti, Mila Pakpahan, Poltak Pardamean, Erikson Sahala Pertiwi, Zuwini Poltak Pakpahan Poltak Pakpahan Purba, Andre Manusun Purba, Yoshua Dewaman Mangapul Putra, Mutlas Ade Putra, Sanjaya Mandala Putri Noviayu Salsabila Hendri Putri, Annisa Dwi Putri, Atika Putri, Mega Novia Putri, Rianti RA, Sayyidina Abdul Qabidhi Rabil Yusuf Rabil Yusuf Rahmadona, Zikra Rahmat Iman Mainil Ramadhanita, Ramadhanita Rebeka Valensia Rhino Pamungkas Riauwaty , Morina Ridho Aldiokta RIDWAN AFFANDI Rika Sinaga Rinaldi Rinaldi Rini Mastuti Rio Persal Anugrah Risman Ferdiansyah Riswan, M Riva Mutiara Rizka Hasanah Rizki Putra Rizvi, Fharisa Nabila Rona Lestari Ronal Kurniawan Roslina &#039; Rudi Alfinda Saberina Hasibuan Sagitha Aulya Fanny Tanjung Salsabila Hendri, Putri Noviayu Santa, Santa Santikawati, Susi Sarah Pabiola Saragih Siallagan, Romensius Anggi AW Siburian, Melin Simanjuntak, Jan Stepfely Sinaga, Tomuso Angdi Suryani Siregar, Cici YP Siregar, Desty Sahroni Siregar, Morina Riauwaty Sri Wahyuni Suci Wulandari Sudirman, Isna Syafriadiman, Syafriadiman Syafruddin Nasution Syahputra, Mohamad Ardani Syahrul Hidayat Syaieba, Mutiara Tamba, Jaya Maruli Tanjung, Yuliza Virliansyah Telaumbanua, Siti Tia Suherianti Tjipto Leksono Tjipto Leksono Tomuso Angdi Suryani Sinaga Trisla Warningsih Tungkup, Mariana Lumban Ulandari, Ulfa Unggul Fitrah Heriadi Usman M Tang Usman M Tang Usman Muhammad Tang Vekri Ramadhi Wasmen Manalu Widarsa, Anang Wijaya, Rahmat Santosa Windarti Windarti Yetti Elfina Yetti Elfina S Yoshua Demawan Mangapul Purba Yosri Yosri Yovanie, Faira Yuharmen - Yuliati Yuliati Yulyawati Yulyawati Yunita Paramitha Hasibuan Yusni Ikhwan Yusni Ikhwan Siregar Yusuf, Rabil Zebua, Ratna Dewi Zikra Rahmadona Zissalwa, Fricilia Zulaiha &#039; Zulkarnain Zulkarnain Zulkarnain Zulkarnain