Claim Missing Document
Check
Articles

Prevention of Motile Aeromonas Septicemia Disease with (Muntingia calabura L) to Striped Catfish (Pangasianodon hypophthalmus) Hafil Kurnia Tama; Iesje Lukistyowati; Henni Syawal
Jurnal Akuakultur SEBATIN Vol. 5 No. 1 (2024): Mei
Publisher : Jurusan Budidaya Perairan, Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.5.1.73-83

Abstract

This research was held from December 2020 – February 2021 at the Laboratory of Fish Parasites and Diseases, Faculty of Fisheries and Marine Science, Universitas Riau. The purpose of this study was to analyze and obtain the best dose of cherry leaf solution to prevent Motile Aeromonas Septicemia from total erythrocytes, hematocrit and hemoglobin levels of striped catfish (Pangasianodon hypophthalmus) fish. The method used to experiment with Completely Randomized Design (CRD) is one factor of five treatment levels. The doses used were negative control without soaking the cherry leaf solution and not tested against Aeromonas hydrophila, positive control without soaking the cherry leaf solution and tested against A. hydrophila, soaking the cherry leaf solution with a dose of 2800 ppm (P1), 3,200 ppm (P2), and 3,600 ppm (P3). Immersion was performed five times for 5 minutes with an interval of 7 days. The results showed that soaking with cherry leaf solution at a dose of 3,600 ppm (P3) was the best dose (P<0.05) with a total value of erythrocytes after the challenge test of 209,66 x 104 cells/mm3, hematocrit level of 37%, hemoglobin 11.86 g/dL, and the survival rate of the test fish was 86,67%.
Isolation of Lactic Acid Bacteria from Intestines of Eel (Monopterus albus) Raised in Biofloc System Cultivation Feri Julianto; Iesje Lukistyowati; Henni Syawal
Jurnal Akuakultur SEBATIN Vol. 4 No. 2 (2023): November
Publisher : Jurusan Budidaya Perairan, Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.4.2.31-41

Abstract

Biofloc technology is one of the most profitable alternative solutions to the problem of aquaculture waste. The research was carried out from July to August 2020 at the Laboratory of Fish Parasites and Diseases, Faculty of Fisheries and Marine Affairs, Riau University. The purpose of this study was to isolate and identify candidate lactic acid bacteria (LAB) that have the potential as probiotics from the intestines Monopterus albus and to determine the growth rate of candidate probiotic bacteria and can suppress the growth of the pathogenic bacteria Aeromonas hydrophila. The method used is the experimental method with treatment of pH 2, pH 4 and pH 6, isolation was carried out from the best Monopterus albus rearing with a stocking density of 30 fish/25 liters of water. Candidate probiotic bacteria was found from the intestines of Monopterus albus as many as 6 LAB candidate isolates based on the results of physical, biochemical and pH treatments, the morphology of the probiotic candidate isolates consisted of one type of bacteria, namely Lactobacillus, from the 6 isolates which showed the widest zone of inhibition against the pathogenic bacteria Aeromonas hydropila was isolate derived from Monopterus albus intestine result shown that which grows at pH 2 is BL 2.1.1 with an inhibition zone diameter of 9,05 mm.
Effectiveness of Feed Feeding Containing Fermented Papaya Leaves (Carica papaya L.) Solutions on The Appearance of Erythrocyte and The Growth of Jambal Siam Fish (Pangasianodon hypophthalmus) Latifah Hannum Lase; Iesje Lukistyowati; Henni Syawal
Jurnal Akuakultur SEBATIN Vol. 3 No. 1 (2022): May
Publisher : Jurusan Budidaya Perairan, Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.3.1.63-77

Abstract

Papaya leaves (Carica papaya L.) contain compounds such as tannins, flavonoids, tocophenols, carpain alkaloids, and papain enzymes which have proteolytic activity, antimicrobial and can be used as an immunomodulators that can strengthen the immune system. The purpose of this study was to obtain the best dose of adding fermented papaya leaf solution to feed to improve the health of jambal siam fish (P. hypophthalmus). The method used is an experimental method using a one-factor Completely Randomized Design (CRD) with five treatment levels and three replications. The treatments used were Kn (Negative Control): The feed was not added with fermented papaya leaf solution and was not tested against Aeromonas hydrophila, Kp (Positive Control): The feed was not added with fermented papaya leaf solution but was tested against A. hydrophila), and feed added with fermented papaya leaf solution at a dose of P1 (16 ml/kg), P2 (18 ml/kg), P3 (20 ml/kg) and challenged with A. hydrophila bacteria. The fish were kept for 46 days. The challenge test of A. hydrophila was carried out on the 32nd day intramuscularly with a density of 108 CFU/ml with a dose of 0.1 ml/head. The results showed that the best dose of fermented papaya leaves added to the feed was found in P3 (20 ml/kg) treatment with a total erythrocyte of 284,33 x 104 cells/mm3, the hematocrit value was 38.67%, and the hemoglobin level was 10.67 g/dL, with weight growth absolute 7.80 g. Water quality ranges from 27 – 30 oC, pH 5.5 – 7, DO 4.3 – 5.8 ppm, and ammonia 0.017 – 0.023 mg/L.
Description of Leukocytes of Jambal Siam (Pangasianodon hypophthalmus) Feed Containing Papaya Leaf Solution (Carica papaya L.) Fermented Dian Puspitowati; Iesje Lukistyowati; Henni Syawal
Jurnal Akuakultur SEBATIN Vol. 3 No. 1 (2022): May
Publisher : Jurusan Budidaya Perairan, Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.3.1.78-92

Abstract

Papaya leaves (Carica papaya L.) contain tannins, alkaloids, flavonoids, terpenoids, saponins, and karpain alkaloids which are antiseptic, anti-inflammatory, antifungal, antibacterial and can be used as immunomodulators that can strengthen the immune system. This study aimed to obtain the best dose of fermented papaya leaf (Carica papaya L.) solution in increasing the non-specific immune response of jambal siam fish by looking at total leukocytes, leukocyte differentiation, phagocytic activity, and survival. The method used is an experimental method using a completely randomized design (CRD) with five levels of treatment, namely (Kn): feeding without being given a fermented papaya leaf solution and without being challenged with A. hydrophila bacteria, (Kp): feeding without being given a leaf solution fermented papaya and challenged with A. hydrophila bacteria, and feed added with fermented papaya leaf solution with doses of P1 (16 ml/kg), P2 (18 ml/kg), P3 (20 ml/kg) and challenged with bacteria A. hydrophila. Fish were reared for 46 days. A. hydrophila challenge test was performed on the 32nd day intramuscularly with a density of 108 CFU/ml as much as 0.1 ml/head. The results showed that fermented papaya leaf solution at a dose of 20 mL/kg of feed was the best dose to increase the non-specific immune response of Jambal Siamese fish with an average total leukocyte of 10.05 x104 cells/mm3, lymphocytes 82.00%, monocytes 4 ,67%, neutrophils 6.67%, platelets 6.67%, phagocytic activity 33.67%, and 96,67% survival
Sensitivity of Terminalia catappa L. Toward Aeromonas hydrophila Andre Manusun Purba; Morina Riauwaty; Henni Syawal
Jurnal Perikanan dan Kelautan Vol. 25 No. 2 (2020): June
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.25.2.166-122

Abstract

Terminalia catappa is commonly present in tropical area and its can be used for increasing pH in fish culture and it also be used as disinfectant. The aimed of this study was to obtain a dose of T. catappa leaf solution which was able to inhibit the growth of Aeromonas hydrophila. This research was conducted from April to August 2019, at the Laboratory of Parasites and Fish Diseases, Department of Aquaculture, Faculty of Fisheries and Marine, Riau University. The method used in this study is an experimental method, namely disk diffusion KIRBY-BAUER to see the sensitivity of T. catappa leaf solution against A. hydrophila. The Kirby-Bauer method uses a 6 mm diameter blank disk, to reduce the level of error, then it is repeated three times. The treatments applied were K: Control (Oxytetracycline) at a dose of 30 µg / disk, P1: Giving T. catappa leaf solution 100% dose, P2 (90%), P3 (80%), P4 (70%), P5 (60%) , P6 (50%), P7 (40%), P8 (30%), P9 (20%), P10 (10%) P11 (9%), P12 (8%), P13 (7%), P14 (6%), P15 (5%), P16 (4%), P17 (3%), P18 (2%), P19 (1%). The results showed that the T. catappa leaf solution was able to inhibit the growth of A. hydrophila bacteria at doses of 100-2% with inhibitory zone diameters of 13.9-6.5 mm.
Erythrocyte Profile of Pangasius hypophthalmus Feed with Rhizophora Apiculata Leaf Extract and Maintained in Net Cages Fricilia Zissalwa; Henni Syawal; Iesje Lukistyowati
Jurnal Perikanan dan Kelautan Vol. 25 No. 1 (2020): Februari
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.25.1.70-78

Abstract

Rhizophora apiculata leaf extract has benefits as an herbal supplement that can be added to feed, because it have componds like flavonoid, tannins, saponins, terpenoids, and steroids that can improve the health of jambal fish (Pangasius hypophthalmus). This research was carried out in August to October 2019. The aim of the research was to obtain the best dose of R. apiculata leaf extract added to feed to improve the health of jambal fish as seen from total erythrocytes, hemoglobin levels, hematocrit levels, and erythrocyte morphology. The method was used an experimental method with a Completely Randomized Design (CRD) one factor of four treatment levels consisting of P0: without the addition of extracts; P1: (1.5 mg/kg); P2: (1.7 mg/kg); and P3: (1.9 mg/kg) and repeated three times so that it takes 12 units of the experiment. Maintenance is carried out for 60 days in cages net with a size of 1x1.5x1 m3 out three times, at the beginning of maintenance, 30th day, and 60th day. The best dose was in the P2 treatment (1.7 mg/kg) seen in total erythrocytes 2.57x106 cells/mm3, hemoglobin level 13.13 g/dL, hematocrit value 38.43%, absolute weight growth 38.93 g, survival rate 97,33%, and showed normal erythrocyte morphology, it was supported by water quality during the reasearch such as DO 6.4-6,9 mg/L, pH 6.1-6.8, temperature 28.2 29.50C, and ammonia 0.04-0.08  mg/L. The Blood tests were carried.
Description of Leukocytes Pangasius hypophthalmus which is Fed with Extracts of Mangrove Leaf (Rhizophora apiculata) and Maintained in The Floating Cages Zikra Rahmadona; Henni Syawal; Iesje Lukistyowati
Jurnal Perikanan dan Kelautan Vol. 25 No. 1 (2020): Februari
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.25.1.79-87

Abstract

Mangrove leaves (R.apiculata) contain flavonoid compounds, terpenoids, steroids, saponins, and tannins which function as antibacterial and can improve fish health. This research was carried out in August to October 2019. This research purpose was to know the proper dose of mangrove leaf (R. apiculata) added to the feed to improve fish health as seen from total leukocytes, leukocyte differentiation, and phagocytic activity of P. hypophthalmus. The method in this research was the experimental method using the Completely Randomized Design (CRD) with one factor, four treatment levels and three times replication, so that it takes 12 units of experiments. The treatments were P0 (feed without additional extract R. apiculata), P1, P2, and P3 feed which were given extract R. apiculata at a dose of 1,5 mg/kg, 1,7 mg/kg and 1,9 mg/kg of feed. The fish used is P. hypophthalmus size 8-10 cm with a stocking density of 50 fish/m3. Feeding three times a day at 08.00, 12.00, and 17.00. Blood testing was performed three times, namely the beginning of the 1st, 30th, and 60th day of maintenance. The results showed that the addition of extract (R. apiculata) into the feed had a significant effect on total leukocytes, leukocyte differentiation, phagocytic activity, survival rate and growth of absolute weight of P. hypophthalmus. The best dose is 1,7 mg/kg of feed with total leukocytes 11,17x104 cells/mm3,  lymphocytes 84,33%, neutrophils 6,67%, monocytes 9,00%, phagocytosis activity 35,00%, survival rate 97,33%, and absolute weight growth of 38,93g. 
Hematology and Physiology of Striped Catfish (Pangasianodon hypophthalmus) with the addition of guava leaf extract (Psidium guajava L) Ilham Fajri Pohan; Morina Riauwaty; Henni Syawal
Jurnal Perikanan dan Kelautan Vol. 29 No. 3 (2024): October
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.29.3.367-374

Abstract

Striped catfish (Pangasianodon hypophthalmus) is a widely cultivated type of superior food fish. Intensive cultivation of catfish can cause disease. Feeding striped catfish with guava leaf extract is expected to reduce stress, which can be seen in striped catfish's hematological and physiological conditions. This research aims to obtain a dose of guava leaf extract (Psidium guajava L) to improve the health of striped catfish. The method used in the study was an experimental method that applied a one-factor, Completely Randomized Design (CRD) with five treatment levels. The treatment used was without adding guava leaf extract (P0); P1, P2, P3, and P4 with doses of 25, 50, 75, and 100 mL/kg feed, and all treatments were tested against Aeromonas hydrophila. The fish used in this research were 250 striped catfish seeds measuring 6-8 cm and kept for 45 days. Feeding with the addition of guava leaf extract at a dose of 75 mL/kg of feed had an influence on the hematological and physiological profile of post-challenge catfish, such as total erythrocytes of 2.40x106 cells/mm3, hematocrit 35.66%, hemoglobin levels 9.61 g/dL, total leukocytes 10.54x 104 cells/mm3, blood glucose levels of 78.00 mg/dL and phagocytic activity 35.66%. Absolute weight growth 17.76 g, feed efficiency 41.07%.
Identification of Pathogenic Bacteria in Cyprinus carpio Reared in Floating Cages Erikson Sahala Pardamean; Henni Syawal; Morina Riauwaty
Jurnal Perikanan dan Kelautan Vol. 26 No. 1 (2021): February
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.26.1.26-32

Abstract

Pathogenic bacteria is disease causing microorganisms that can attack fish and can cause mass death in cultured fish. The purpose of this study was to identify the types of pathogenic bacteria Cyprinus carpio that were reared in floating cages. This study used a survey method that is purposive sampling and the fish sample were obtained from, PLTA Koto Panjang. the fishes were identified in the Fish Parasite and Disease Laboratory, Fish and Marine Faculty, University of Riau. Fish samples used were 15-20 cm goldfish totaling 12 tails with 3 times taken from 4 different cages. The kidneys organ were examined and to find out the type the bacteria. Result shown identified of pathogenic bacteria consist of 3 types of bacteria namely Aeromonas sp., Pseudomonas sp., and Edwardsiella sp. Water quality during the study was Temperature: 27-300C, pH 6-7, DO 3.67-4.28 ppm, and ammonia 0.048-0.64 mg/L.
Identification of Pathogenic Bacteria from Striped Catfish (Pangasionodon hypophthalmus) kept in Aquaculture Ponds Jaya Maruli Tamba; Henni Syawal; Iesje Lukistyowati
Jurnal Perikanan dan Kelautan Vol. 26 No. 1 (2021): February
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.26.1.40-46

Abstract

Koto Masjid Village is the largest production center in striped catfish fish farming activities in Riau Province. One of the inhibiting factors is the disease caused by bacteria, this study aims to determine the types of pathogenic bacteria that have the potential to infect striped catfish. This research was conducted in August - November 2019, samples of striped catfish used in the study amounted to 15 tails with an average length of 15-25 cm taken from 5 ponds in Kampung II, Koto Masjid Village, XIII Koto Kampar Subdistrict, and then identified bacteria by Conventional Laboratory in Fish Parasites and Diseases, Faculty of Fisheries and Marine, Riau University, Pekanbaru. This study uses a survey method that is by taking samples from the field in Pu and then the isolation of bacteria is carried out from the kidney organ in the TSB media then purified on the TSA media. The results of the study found 3 types of pathogenic bacteria namely Aeromonas sp., Edwardsiella sp., and Pseudomonas sp. Water quality during the study was pH 6.6-70, DO 4.05-4.35 mg/L, NH3 0.035-0.2 mg/L and Temperature 28-300C.
Co-Authors Abdul Alif Abdul Alif Addilah, M. Fikri Adelina &#039; Afra Azizah Afrina Putri Afrizal Tanjung Alfian &#039; Alfinda, Rudi Alif, Abdul Amraini Fitri Anang Widarsa Andarini Diharmi Anderson.s, Roi Andesra, Andesra Andi Almer Farrand Noor Andre A Saragih, Andre A Andre Manusun Purba Anggriani, Rara Dewi Angraika Dirta Annisa Dwi Putri Anugrah, Rio Persal Anzila Rizki W Muharram Asmika Harnalin Simarmata Athif Amirah Elza Atika Talitha Erman Atria Martina Azizah, Afra Cerlina, Maya Chandra Chandra Chania Arnata Christin Natalia Cici Adliana Darfia, Novreta Dea Switzelin H Destriman Laoli Devy P Lumban Gaol Dian Fitria M Dian Iriani Dian Puspitowati Dicky Azwar Lubis Dinar Mey Jasika H Diva, Farhah Dwi Indah Fransiska Elfira Stefani Priska Erikson Sahala Pardamean Etri Nopilita Faira Yovanie Fandi Rahmat Farkhan, Mochammad Farrand Noor, Andi Almer Fatihaturrahma, Annisya Fauzi Ahmad Lubis Faza, Farzana Fakhira Fazira, Meisya Amanda Febi Nabila Feri Julianto Fitria Sufriyani Fransiska, Dwi Indah Fricilia Zissalwa Gaol, Devy P Lumban Ginting, Kiki Dirgantara Gunawan B S Gusriansyah, Dimas Hafil Kurnia Tama Hasbullah Zakky Hasugian, Sampang Hotramadi Herdini , Herdini Herianti Rambe Heriyanti Heriyanti, Heriyanti Herparinda Novita Husna, Hidayatul Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukstyowati Iesje Lukystiowati Ilham Fajri Pohan Iman, Khasan Nur Ipa, Adha Irma Yunita Irsya, Habib Irwan Effendi Iskandar Putra Isma Mulyani Isna Sudirman Isna Sudirman Isnaini Lidya Rizki Jampil, Tirta Herlis Jan Stepfely Simanjuntak Jaya Maruli Tamba Juniarty Manurung Juwita Lestari Karnila, Rahman Karnila Khairia, Khairia Kiky Dirgantara Ginting Kurniawan, Ahmad Juli Kusai, Kusai Latifah Hannum Lase Lestari, Rona Luksityowati, Iesje Luskityowati, Iesje M Riswan M Zaky Ditiya Putra M. Riswan M. Yanda, M. Yanda Manurung, Juniarty Mariana Lumban Tungkup Mariska, Refi Marwati &#039; Maulina Sawitri Maya Cerlina Maytra Wirdona Meisya Amanda Fazira Melin Siburian Memi Martin Ilmayati Mery Sukmiwati Mesi Susanti Mochammad Farkhan Morina Riauwaty Muhamad Latiful Khobir Muhammad Akram Muhammad Arga Saputra Muhammad Arga Saputra Muhammad Arga Saputra Muhammad Azaldin Muhammad Muhajir Muhammad Muhajir, Muhammad Mutawakkil, M. Mufid Al Mutiara Mutiara Nabellia Nabellia Nabellia, Nabellia Nabila, Febi Nasril Ismail NASTITI KUSUMORINI Nelli Karolina Sitorus Novike Zullanda Selvi, Novike Zullanda Novita, Herparinda Novreta Ersyi Darfia Nur Addini Nur Aini Nur Asiah Nuraini Nuraini Nuraini Nuraini Nuraini Nuraini Nurfitri, Maya Nurhasan Nurhasan Nurhasan Nurhasan, Nurhasan Oktavianti, Mila Pakpahan, Poltak Pardamean, Erikson Sahala Pertiwi, Zuwini Poltak Pakpahan Poltak Pakpahan Purba, Andre Manusun Purba, Yoshua Dewaman Mangapul Putra, Mutlas Ade Putra, Sanjaya Mandala Putri Noviayu Salsabila Hendri Putri, Annisa Dwi Putri, Atika Putri, Mega Novia Putri, Rianti RA, Sayyidina Abdul Qabidhi Rabil Yusuf Rabil Yusuf Rahmadona, Zikra Rahmat Iman Mainil Ramadhanita, Ramadhanita Rebeka Valensia Rhino Pamungkas Riauwaty , Morina Ridho Aldiokta RIDWAN AFFANDI Rika Sinaga Rinaldi Rinaldi Rini Mastuti Rio Persal Anugrah Risman Ferdiansyah Riswan, M Riva Mutiara Rizka Hasanah Rizki Putra Rizvi, Fharisa Nabila Rona Lestari Ronal Kurniawan Roslina &#039; Rudi Alfinda Saberina Hasibuan Sagitha Aulya Fanny Tanjung Salsabila Hendri, Putri Noviayu Santa, Santa Santikawati, Susi Sarah Pabiola Saragih Siallagan, Romensius Anggi AW Siburian, Melin Simanjuntak, Jan Stepfely Sinaga, Tomuso Angdi Suryani Siregar, Cici YP Siregar, Desty Sahroni Siregar, Morina Riauwaty Sri Wahyuni Suci Wulandari Sudirman, Isna Syafriadiman, Syafriadiman Syafruddin Nasution Syahputra, Mohamad Ardani Syahrul Hidayat Syaieba, Mutiara Tamba, Jaya Maruli Tanjung, Yuliza Virliansyah Telaumbanua, Siti Tia Suherianti Tjipto Leksono Tjipto Leksono Tomuso Angdi Suryani Sinaga Trisla Warningsih Tungkup, Mariana Lumban Ulandari, Ulfa Unggul Fitrah Heriadi Usman M Tang Usman M Tang Usman Muhammad Tang Vekri Ramadhi Wasmen Manalu Widarsa, Anang Wijaya, Rahmat Santosa Windarti Windarti Yetti Elfina Yetti Elfina S Yoshua Demawan Mangapul Purba Yosri Yosri Yovanie, Faira Yuharmen - Yuliati Yuliati Yulyawati Yulyawati Yunita Paramitha Hasibuan Yusni Ikhwan Yusni Ikhwan Siregar Yusuf, Rabil Zebua, Ratna Dewi Zikra Rahmadona Zissalwa, Fricilia Zulaiha &#039; Zulkarnain Zulkarnain Zulkarnain Zulkarnain