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Identification of Pathogenic Bacteria from Striped Catfish (Pangasionodon hypophthalmus) kept in Aquaculture Ponds Tamba, Jaya Maruli; Syawal, Henni; Lukistyowati, Iesje
Jurnal Perikanan dan Kelautan Vol. 26 No. 1 (2021): February
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Koto Masjid Village is the largest production center in striped catfish fish farming activities in Riau Province. One of the inhibiting factors is the disease caused by bacteria, this study aims to determine the types of pathogenic bacteria that have the potential to infect striped catfish. This research was conducted in August - November 2019, samples of striped catfish used in the study amounted to 15 tails with an average length of 15-25 cm taken from 5 ponds in Kampung II, Koto Masjid Village, XIII Koto Kampar Subdistrict, and then identified bacteria by Conventional Laboratory in Fish Parasites and Diseases, Faculty of Fisheries and Marine, Riau University, Pekanbaru. This study uses a survey method that is by taking samples from the field in Pu and then the isolation of bacteria is carried out from the kidney organ in the TSB media then purified on the TSA media. The results of the study found 3 types of pathogenic bacteria namely Aeromonas sp., Edwardsiella sp., and Pseudomonas sp. Water quality during the study was pH 6.6-70, DO 4.05-4.35 mg/L, NH3 0.035-0.2 mg/L and Temperature 28-300C.
Description of Erythrocytes of Striped Catfish (Pangasionodon hypophthalmus) Infected by Edwardsiella tarda Treated with Mango Leaf (Mangifera indica) Tungkup, Mariana Lumban; Syawal, Henni; Riauwaty, Morina
Jurnal Perikanan dan Kelautan Vol. 26 No. 1 (2021): February
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

This research has been carried out from November to December 2019 at the Laboratory of Parasites and Fish Diseases at the Fisheries and Marine Faculty of Riau University. The aim of the study was to obtain the best dose of mango leaf solution for the treatment of striped catfish (P. hypophthalmus) infected with E. tarda, seen from the erythrocyte picture. The method used was an experiment with a Completely Randomized Design (CRD) of a factor of five treatment levels and replications three times. Test fish used were striped catfish measuring 8-12 cm, with a total of 150 fish. The treatments used for treatment were 1000 ppm, 1200 ppm, 1400 ppm, negative controls (not infected and not treated), and positive controls (infected and not treated). Parameters measured and observed among others; total erythrocytes, hemoglobin levels, hematocrit values, erythrocyte cell morphology and survival rate. The results showed that apple mango leaf solution (M. indica) was able to treat conjoined striped catfish infected with E. tarda by Emmersion method. The best treatment of 1200 ppm is characterized by an average total erythrocyte, mean total erythrocytes 149.33 x 104 cells / mm3, hemoglobin level 9.03 g / dL, hematocrit value 35.67% and the survival of the test fish after treatment is 86.67%. The results of water quality measurements during the study, namely temperature 27.00-27.46 0C, pH 6.1-6.77, dissolved oxygen 3.53-3.87 mg/L, and ammonia 0.02 mg/L.
THE EFFECT OF Moringa oliefera LEAF FERMENTED ENRICHED PELLETS ON HEMATOLOGY OF Pangasianodon hypophthalmus REARED IN PHOTOPERIOD SYSTEM Gusriansyah, Dimas; Syawal, Henni; Riauwaty, Morina
Asian Journal of Aquatic Sciences Vol. 8 No. 1 (2025): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/ajoas.8.1.41-52

Abstract

Moringa leaf (Moringa oleifera) is a herbal plant that has the potential to inhibit bacterial growth and increase immune response. This research was conducted from April to July 2024 at the Biotechnology Laboratory, Faculty of Fisheries and Marine Sciences, Universitas Riau. This study aims to obtain the best dose of fermented moringa leaf added to feed to the immunity of Siamese striped catfish reared on a photoperiod system and tested with A. hydrophila. The research method is the experimental method applying a completely randomized design (CRD) with four treatments and three replicates. Treatment 0 without adding fermented moringa leaves, P1: 10 g/kg feed, P2: 15 g/kg feed, and P3: 20 g/kg feed and challenged with A. hydrophila. The test fish samples measuring 6-7 cm were reared using 100 L buckets of 12 containers with a 30 fish/container stocking density. Feeding was done ad satiation with a frequency of 3 times daily at 08.00, 13.00 and 17.00 WIB. The results showed that adding fermented moringa leaf to the feed affected the immunity of striped catfish. The best dose was found in P1 (10 g/kg feed) with total erythrocytes 2.75 x 106 cells/mm3, hemoglobin level 8.80 g/dL, hematocrit level 34.00%, and total leukocytes 10.86 x 104 cells/mm3, leukocrit level 3.33%, lymphocytes 76.00%, monocytes 7.33%, neutrophils 7.33%, Thrombocytes 9.33%, absolute weight growth 15.02 g/fish, absolute length growth 6.88 cm/fish, 96.67% survival rate. Based on the study's results, feeding added fermented moringa leaves has a significant effect and can improve the immunity of Pangasinodon hypophthalmus
EFFECT OF rGH HORMONE ADMINISTRATION ON PROTEASE ENZYME ACTIVITY AND RNA/DNA RATIO OF STRIPED CATFISH (Pangasianodon hypophthalmus) MAINTAINED IN SALINE MEDIA Syahputra, Mohamad Ardani; Tang, Usman M; Syawal, Henni
Asian Journal of Aquatic Sciences Vol. 7 No. 1 (2024): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Striped catfish is a prevalent fish and is in great demand. Striped catfish cultivation generally uses fresh water as a living medium, but several freshwater fish commodities are starting to be developed to adapt to saline media. Harvesting striped catfish usually takes approximately 3 - 8 months. To overcome this, one way is to use growth hormone. The growth hormone currently used is Recombinant Growth Hormone (rGH). GH administration has been reported to have various effects, particularly on protein metabolism. In fish digestion, protein from feed cannot be directly absorbed but is first broken down by protease enzymes into amino acids. The physical appearance, which is the phenotype of an organism, is the result of metabolic processes in each cell that makes up the organism. This research aimed to analyze the effect of administering the rGH hormone on protease enzyme activity, RNA/DNA ratio, and growth of striped catfish maintained in saline media. The method used was experimental in the form of a Completely Randomized Design (CRD) with four treatments and three replications. Based on the results obtained during the research, it shows that Protease Enzyme Activity (U/minute) and the best RNA/DNA Ratio use a dose of 3 mg/kg with respective values ​​of 0.75±0.24 U/minute and3.30±0.49.
THE USE OF Kaempferia galanga AND Curcuma xanthorrhiza FERMENTED TO IMPROVE THE IMMUNITY OF Oreochromis niloticus AGAINST Streptococcosis Khairia, Khairia; Syawal, Henni; Luksityowati, Iesje; Riauwaty, Morina
Asian Journal of Aquatic Sciences Vol. 6 No. 3 (2023): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Streptococcus agalactiae is a bacterium that causes streptococcosis, which attacks tilapia. This research was conducted from March to June 2022 at the Laboratory of Parasites and Fish Diseases, Faculty of Fisheries and Marine, University of Riau. This study aims to understand the best dose of fermented Kaempferia galanga and Curcuma xanthorrhiza, which are added to the feed to increase Oreochromis niloticus immunity. The method used was experimental with a completely randomized design (CRD) with five treatment levels and three replications. The treatments applied were Kn: Negative control (no treatment used), Kp: positive control (no treatment used and infected with S.agalactiae), P1 (100 mL/kg feed), P2 (125 mL/kg feed), P3 (100 mL/kg feed) and infected with S. agalactiae. The fish were contaminated on the 32nd day at a density of bacteria 108 CFU/mL of 0.1 mL/fish intramuscularly, and then the fish were reared up to the 46th day. The results showed that the fermented K.galanga and C.xanthorrhiza affected the haematology of O.niloticus. The best dose was in P3 with a dose of 150 ml/kg of feed which showed total erythrocytes of 2.01 x106 cells/mm3, hematocrit value of 27.33%, a hemoglobin level of 7.93 g/dL, total leukocytes of 6.59 x104 cells/mm3, lymphocytes 77.33%, monocytes 14.00%, neutrophils 8.67%, growth with a weight of 34.06 g, a length of 7.5 cm and a survival rate of 93.33%. Based on the data obtained, it can be concluded that fermented K.galanga and C.xanthorrhiza can increase the immunity of O.niloticus against Streptococcosis disease
THE EFFECT OF SALINITY ON THE PHYSIOLOGICAL PERFORMANCE OF ASIAN REDTAIL CATFISH SEEDS (Hemibagrus nemurus) Putra, Mutlas Ade; Tang, Usman M; Syawal, Henni
Asian Journal of Aquatic Sciences Vol. 6 No. 3 (2023): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

This research was conducted 30 days from June to July 2022 at the Aquaculture Laboratory, Faculty of Fisheries and Marine Sciences, Universitas Riau, Pekanbaru. This study aimed to analyze the osmoregulation response of the Asian redtail catfish to different salinities and to obtain optimal salinity for good physiological performance for the Asian redtail catfish. The method used was an experimental method using a completely randomized design (CRD) with five treatments and four replications, namely, three ppt salinity treatment, six ppt salinity treatment, nine ppt salinity treatment, 12 ppt salinity treatment, and 15 ppt salinity treatment. The test fish used were Asian redtail catfish with an average size of 7.17±0.04 cm. Asian redtail catfish seeds were obtained from the Tibun Fish Seed Center, Kampar. The container used is an aquarium with a size of 60x40x40 cm. Research data shows that nine ppt salinity is the best for Asian redtail catfish osmoregulation of 0.5 mOsm/L H2O. The best cortisol hormone content is found at three ppt salinity, the lowest oxygen consumption is at three ppt salinity of 0.9 mgO2/g/hour, the best survival was found at three ppt salinity of 100%, and the growth in weight and length was in the three ppt treatment of 8.90 g and 2.04 cm. Water quality parameters include temperature 25-30oC and dissolved oxygen 5.04-8.04 ppm.
PROFILE ERYTHROCYTES OF Oreochromis niloticus FED WITH HERBS FERMENTED AND INFECTED WITH Aeromonas hydrophila Nurfitri, Maya; Syawal, Henni; Lukistyowati, Iesje; Riauwaty, Morina
Asian Journal of Aquatic Sciences Vol. 6 No. 3 (2023): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Herbs Fermented consist of Kaempferia galanga L, Curcuma xanthorrhiza Roxb, and Curcuma domestica Val, which are environmentally friendly disease prevention alternatives that can be added to feed because they have several advantages, such as increasing appetite, digestion, growth, disease resistance, and reducing stress levels in fish. This research was carried out from August to December 2021 at the Marine Science Biotechnology Laboratory and Fish Parasites and Diseases Laboratory, Faculty of Fisheries and Marine, Universitas Riau. This study aimed to obtain the best dose from adding fermented herbs to increase the immune system of Oreochromis niloticus and prevent diseases caused by Aeromonas hydrophila bacteria in terms of profile erythrocytes. The method used was an experimental method using a Completely Randomized Design (CRD) with one factor, five levels of treatment, and three replications so that 15 experimental units were obtained. The challenge test was carried out on the 32nd day using A.hydrophila with a density of 108 CFU/mL. The best dose was found in P3 with a dose of 200 mL/kg of feed, which showed a total erythrocyte of 2.09x106 cells/mm3, hemoglobin level of 7.70 g/dL, hematocrit value of 37.70%, and a normal erythrocyte morphology. Water quality ranged from temperature 27-29oC, pH 6.9-7.2, DO 3.1-4.4 mg/L, and Ammonia 0.016-0.024 mg/L. Based on the data obtained, it can be concluded that fermented herbs can increase the immunity of O. niloticus to diseases caused by A.hydrophila.
HEMATOLOGY OF ASIAN REDTAIL CATFISH (Hemibagrus nemurus) REARED IN MEDIA WITH REGULAR ADDITION OF PROBIOTICS Lestari, Rona; Syawal, Henni; Lukistyowati, Iesje
Asian Journal of Aquatic Sciences Vol. 6 No. 1 (2023): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Multi-cell probiotic containing bacteria Nitrosomonas sp., Nitrobacter sp., and Bacillus sp. This research was conducted from March to May 2021 at the Fish Disease and Parasite Laboratory, Department of Aquaculture, Faculty of Fisheries and Marine, Universitas Riau. This study aimed to analyze the hematology of Asian redtail catfish reared in media that was added with probiotics periodically and to obtain the best time interval for giving probiotics to the rearing medium. The method used in this study is an experimental method by applying a Completely Randomized Design (CRD) with five treatment levels and three replications. The treatments used were the administration of probiotics at a dose of 0,01 mL/L of water with different time intervals, P0 (without treatment), P1 (5 days once), P2 (10 days once), P3: (15 days once), and P4 (20 days once). Fish were reared for 50 days. The results show that the Asian redtail catfish reared with the addition of probiotics in the rearing medium had a significant effect (P<0.05) with the best time interval being once every 5 days in the P1 treatment, seen from the total erythrocytes 2.43 x 106 cells/mm3, hematocrit level 28.67%, hemoglobin level 7.40 g/dL, total leukocytes 9.92 x 104 cells/mm3, leukocrit level 1.83%, and glucose 49.67 g /dL. Water quality during the study was still in the normal range that could support the growth of Asian redtail Catfish, namely temperature 26-27.1 C, pH 5.8-6.5, DO 6.0-7.4 mg/L, and ammonia 0.0011-6.5 0.0047 mg/L.
INTESTINE AND LIVER HISTOPATHOLOGY OF STRIPED CATFISH (Pangasianodon hypophthalmus) FEEDING CONTAINING PAPAYA LEAF FERMENTATION Purba, Yoshua Dewaman Mangapul; Syawal, Henni; Lukistyowati, Iesje
Asian Journal of Aquatic Sciences Vol. 6 No. 1 (2023): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Aeromonas hydrophila bacteria often attack internal organs such as the liver and intestines. This research was conducted in June – August 2021 at the Laboratory of Fish Parasites and Diseases, Faculty of Fisheries and Marine, Universitas Riau. Histology preparations were made at the Bukittinggi Veterinary Center. This research aimed to obtain the best dose addition of papaya leaf fermented solution which could reduce intestinal and liver tissue damage of striped catfish (P.hypophthalmus) which was challenged with A.hydrophila. The method used is an experimental method with a completely randomized design (CRD) with 4 treatment levels and 3 replications; Kp (Feed not given papaya leaf fermented solution + infected with A.hydrophila), P1 (16 ml/kg feed of papaya leaf fermented solution + infected with A.hydrophila), P2 (16 ml/kg feed of papaya leaf fermented solution) + infected with A.hydrophila), P3 (16 ml/kg of papaya leaf fermented solution feed + infected with A. hydrophila). The fish used was 8-10 cm with a density of 1 fish/3 L totaling 10 fish with a container measuring 40x30x30 cm. Infection by injection in the intramuscular section as much as 0.1 mL/head with a bacterial density of 108 CFU/mL. The parameters observed were clinical symptoms for 72 hours and histopathology of the intestine and liver of striped catfish. The results showed that the intestine saw the presence of hemorrhage, necrosis, and edema. While the liver shows abnormalities, such as hemorrhage, necrosis, hypertrophy, and fatty degeneration.
WATER QUALITY IMPROVEMENT AND ANALYSIS FOR CLOWN FISH (Amphiprion ocellaris) CULTIVATION IN A RECIRCULATION SYSTEM Santikawati, Susi; Syafriadiman, Syafriadiman; Syawal, Henni
Asian Journal of Aquatic Sciences Vol. 6 No. 1 (2023): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

The demand for ornamental fish is currently increasing while the availability in nature is decreasing due to overfishing. Clownfish (Amphiprion ocellaris) is a type of marine ornamental fish that is widely kept in aquariums, but the problem is the rapid decline in water quality due to the activity of these fish. The recirculation system is one way that can be used in water quality management. The study aimed to analyze the type of filter used to stabilize seawater quality in clownfish (rearing containers. This research was conducted from January to April 2021 at the Research Centre of Sibolga Fisheries College. The method used was an experimental method using a one-factor Completely Randomized Design (CRD) with four treatment levels and three replications. The treatments in this study were P0 (unfiltered), P1 (cotton filter), P2 (fiber filter), and P3 (activated charcoal filter). The fish used was 2 – 3 cm in size, kept in an aquarium measuring 60x40x40cm with a stocking density of 2 fish/L of water and fed tubifex 2 times a day, at 08.00 and 18.00 WIB as much as 5% of their body weight. Parameters measured were temperature, pH, DO, salinity, ammonia, and phosphate, abundance of bacteria, phytoplankton, and survival of clownfish. Water quality measurements are carried out every 7 days. The results obtained from the improvement and analysis of water quality for the cultivation of clownfish in the recirculation system provides an influence on water quality in the rearing container. The results obtained were temperature 27.9–28.0 0C, pH 8.3–8.4, DO 7.5–8.0 mg/L, salinity 33-34 ppt, ammonia 0.04–0.06 mg/L, phosphate 0.47–0.48 mg/L and bacterial abundance 2.35x104 CFU/mL with a survival rate of 95.55%.
Co-Authors Abdul Alif Abdul Alif Addilah, M. Fikri Adelina &#039; Adelina, Adelin Afra Azizah Afrina Putri Afrizal Tanjung Alfian &#039; Alfinda, Rudi Alif, Abdul Amraini Fitri Anang Widarsa Andarini Diharmi Anderson.s, Roi Andesra, Andesra Andi Almer Farrand Noor Andre A Saragih, Andre A Andre Manusun Purba Anggriani, Rara Dewi Angraika Dirta Annisa Dwi Putri Anugrah, Rio Persal Anzila Rizki W Muharram Aprianti, Rahma Asmika Harnalin Simarmata Athif Amirah Elza Atika Talitha Erman Atria Martina Azizah, Afra Cerlina, Maya Chandra Chandra Chania Arnata Christin Natalia Cici Adliana Darfia, Novreta Dea Switzelin H Destriman Laoli Devy P Lumban Gaol Dian Fitria M Dian Iriani Dian Puspitowati Dicky Azwar Lubis Dinar Mey Jasika H Diva, Farhah Dwi Indah Fransiska Elfira Stefani Priska Eni Sumiarsih Erikson Sahala Pardamean Etri Nopilita Faira Yovanie Fandi Rahmat Farkhan, Mochammad Farrand Noor, Andi Almer Fatihaturrahma, Annisya Fauzi Ahmad Lubis Faza, Farzana Fakhira Fazira, Meisya Amanda Febi Nabila Feri Julianto Fitria Sufriyani Fransiska, Dwi Indah Fricilia Zissalwa Gaol, Devy P Lumban Ginting, Kiki Dirgantara Gunawan B S Gusriansyah, Dimas Hafil Kurnia Tama Hasbullah Zakky Hasugian, Sampang Hotramadi Herdini , Herdini Herianti Rambe Heriyanti Heriyanti, Heriyanti Herparinda Novita Husna, Hidayatul Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukstyowati Iesje Lukystiowati Ilham Fajri Pohan Iman, Khasan Nur Ipa, Adha Irma Yunita Irsya, Habib Irwan Effendi Iskandar Putra Isma, Muhammad Fauzan Isna Sudirman Isna Sudirman Isnaini Lidya Rizki Jampil, Tirta Herlis Jan Stepfely Simanjuntak Jaya Maruli Tamba Juniarty Manurung Juwita Lestari Karnila, Rahman Karnila Khairia, Khairia Kiky Dirgantara Ginting Kurniawan, Ahmad Juli Kusai, Kusai Latifah Hannum Lase Lestari, Rona Luksityowati, Iesje Luskityowati, Iesje M Riswan M Zaky Ditiya Putra M. Riswan M. Yanda, M. Yanda Manurung, Juniarty Mariana Lumban Tungkup Mariska, Refi Marwati &#039; Masjudi, Heri Maulina Sawitri Maya Cerlina Maytra Wirdona Meisya Amanda Fazira Melin Siburian Memi Martin Ilmayati Mery Sukmiwati Mesi Susanti Mochammad Farkhan Morina Riauwaty Muhamad Latiful Khobir Muhammad Akram Muhammad Arga Saputra Muhammad Arga Saputra Muhammad Arga Saputra Muhammad Azaldin Muhammad Muhajir Muhammad Muhajir, Muhammad Mulyadi Mulyadi Mutawakkil, M. Mufid Al Mutiara Mutiara Nabellia Nabellia Nabellia, Nabellia Nabila, Febi Nasril Ismail NASTITI KUSUMORINI Nelli Karolina Sitorus Niken Ayu Pamukas Novike Zullanda Selvi, Novike Zullanda Novita, Herparinda Novreta Ersyi Darfia Nur Addini Nur Aini Nur Asiah Nuraini Nuraini Nuraini Nuraini Nuraini Nuraini Nurfitri, Maya Nurhasan Nurhasan Nurhasan Nurhasan, Nurhasan Oktavianti, Mila Pakpahan, Poltak Panggabean, Devi Yunita Pardamean, Erikson Sahala Pertiwi, Zuwini Poltak Pakpahan Poltak Pakpahan Pristiana, Aris Purba, Andre Manusun Purba, Yoshua Dewaman Mangapul Putra, Mutlas Ade Putra, Sanjaya Mandala Putri Noviayu Salsabila Hendri Putri, Annisa Dwi Putri, Atika Putri, Mega Novia Putri, Rianti RA, Sayyidina Abdul Qabidhi Rabil Yusuf Rabil Yusuf Rahmadona, Zikra Rahmat Iman Mainil Ramadhanita, Ramadhanita Rebeka Valensia Rhino Pamungkas Riauwaty , Morina Ridho Aldiokta RIDWAN AFFANDI Rika Sinaga Rinaldi Rinaldi Rini Mastuti Rio Persal Anugrah Risman Ferdiansyah Riswan, M Riva Mutiara Rizka Hasanah Rizki Putra Rizvi, Fharisa Nabila Rona Lestari Ronal Kurniawan Roslina &#039; Rudi Alfinda Saberina Hasibuan Sagitha Aulya Fanny Tanjung Salsabila Hendri, Putri Noviayu Santa, Santa Santikawati, Susi Sarah Pabiola Saragih Siallagan, Romensius Anggi AW Siburian, Melin Sihaloho, Rosalinda Simalango, Cindy Naomi Fadilla Christine Simanjuntak, Jan Stepfely Sinaga, Tomuso Angdi Suryani Siregar, Cici YP Siregar, Desty Sahroni Siregar, Morina Riauwaty Sri Wahyuni Suci Wulandari Sudirman, Isna Syafriadiman, Syafriadiman Syafruddin Nasution Syahputra, Mohamad Ardani Syahrul Hidayat Syaieba, Mutiara Tamba, Jaya Maruli Tanjung, Yuliza Virliansyah Telaumbanua, Siti Tia Suherianti Tjipto Leksono Tjipto Leksono Tomuso Angdi Suryani Sinaga Trisla Warningsih Tungkup, Mariana Lumban Ulandari, Ulfa Unggul Fitrah Heriadi Usman M Tang Usman M Tang Usman Muhammad Tang Vekri Ramadhi Wasmen Manalu Widarsa, Anang Wijaya, Rahmat Santosa Windarti Windarti Yetti Elfina Yetti Elfina S Yoshua Demawan Mangapul Purba Yosri Yosri Yovanie, Faira Yuharmen - Yuliati Yuliati Yulyawati Yulyawati Yunita Paramitha Hasibuan Yusni Ikhwan Yusni Ikhwan Siregar Yusuf, Rabil Zebua, Ratna Dewi Zikra Rahmadona Zissalwa, Fricilia Zulaiha &#039; Zulkarnain Zulkarnain Zulkarnain Zulkarnain