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Histopathology Intestines of Trichogaster trichopterus Pall Infected by Digenean Trematode Parasites (Clinostomum sp.) Panggabean, Devi Yunita; Riauwaty, Morina; Syawal, Henni
Jurnal Akuakultur SEBATIN Vol. 6 No. 2 (2025): November
Publisher : Department of Aquaculture, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.6.2.66-73

Abstract

Trichogaster trichopterus Pall is a freshwater fish widely found in Indonesian waters, particularly in the swamp areas of Riau Province. This study was conducted from September 2024 to March 2025 at the Laboratory of Fish Parasites and Diseases, Faculty of Fisheries and Marine Science, University of Riau. The objective of this research was to analyze the intestinal tissue structure of T. trichopterus infected with Clinostomum sp. The methods used were purposive sampling and survey techniques. Fish samples were collected from the reservoir at the Faculty of Fisheries and Marine Science, University of Riau. Sampling was conducted three times over 30 days at four predetermined stations. Station I was located at the reservoir entrance, Station II to the west, Station III to the north of the entrance, with a 50-meter distance between stations, and Station IV was located upstream. The research procedures included sampling, intestinal organ fixation in formalin, and preparation of histopathological slides through a series of stages: dehydration, alcohol removal, infiltration, embedding, sectioning, staining, and mounting. Observed parameters included clinical symptoms and intestinal histological structure, which were analyzed descriptively. The results showed that the intestines of infected fish exhibited alterations, including epithelial lifting, goblet cell proliferation, oedema, hypertrophy, haemorrhage, inflammatory cell infiltration, and necrosis. The histopathological index of infected fish intestines reached a damage score of 7, categorised as severe. Based on the findings, it can be concluded that Clinostomum sp. infection causes significant alterations to the intestinal structure of T. trichopterus
Erythrocyte Profile of Three Spot Gourami (Trichogaster trichopterus PALL) Infected by Digenetic Trematode Parasite (Clinostomum sp.) Iqbal, Muhammad; Riauwaty, Morina; Syawal, Henni
Jurnal Akuakultur SEBATIN Vol. 7 No. 1 (2026): Mei
Publisher : Department of Aquaculture, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jas.7.1.37-47

Abstract

The three spot gourami (Trichogaster trichopterus) is a highly sought-after commodity by the community, often processed into salted fish or consumed fresh.. Clinostomum sp. infection, also known as "yellow grub", is a disease in fish caused by the digenea trematode parasite. This parasite is zoonotic, which means it can be transmitted to humans, especially through consumption of raw or undercooked freshwater fish. In humans, this infection can cause laryngo-pharyngitis (inflammation of the throat) and in severe cases, can cause death due to lack of oxygen (asphyxia). The study was conducted from September 2023 to January 2025, at the Laboratory of Parasites and Fish Diseases, Faculty of Fisheries and Marine Sciences, Universitas Riau. The purpose of the study was to determine the erythrocyte profile of marsh gourami infected with the digenea trematode parasite (Clinostomum sp.). The method used in this study was the purposive sampling survey method. Sampling was carried out at the FPK UNRI Reservoir with 3 location points and the total number of sample fish was 180. The results showed that fish infected with Clinostomum sp. had an erythrocyte count of 0.84–1.07 × 10⁶ cells/mm³, a hemoglobin level of 4.29–4.54 g/dL and a hematocrit value of 21.7–27.3%. The range of water quality in the FPK reservoir during the study was a temperature of 26.0-31.2oC, pH ranging from 4.2to 6.1 and DO values ranging from 4.3-6.8 mg/L. Based on the data obtained, it can be concluded that Clinostomum sp. infection can affect fish health
Potential of Red Dragon Fruit (Hylocereus polyrhizus) to Prevent Aeromoniasis in Striped Catfish (Pangasianodon hypophthalmus) Tiara Oktari; Morina Riauwaty; Henni Syawal
Jurnal Perikanan dan Kelautan Vol. 31 No. 2 (2026): June
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.31.2.152-162

Abstract

Red dragon fruit peel is a natural material with antibacterial and antifungal properties, owing to its active compounds, including alkaloids, flavonoids, niacin, tannins, and vitamin C. These compounds can inhibit the growth of Aeromonas hydrophila and enhance fish immunity. This research was carried out from January to August 2025 at the Parasite and Fish Disease Laboratory, Faculty of Fisheries and Marine Sciences, Universitas Riau. This study aimed to examine the effectiveness of red dragon fruit peel solution (Hylocereus polyrhizus) in preventing Aeromoniasis in striped catfish (Pangasianodon hypophthalmus). The experiment used a Completely Randomized Design (CRD) with five treatments and three replications. Treatments consisted of KN: negative control (without immersion and not infected with A. hydrophila), KP: positive control (without immersion and infected with A. hydrophila), P1 (immersion at 1 ml/L and infected), P2 (immersion at 1.5 mL/L and infected), and P3 (immersion at 2 ml/L and infected). Fish were immersed in the peel solution for 30 minutes daily for three consecutive days. Blood sampling was conducted at the beginning of maintenance, on the 30th day, and on the 14th day after infection. The findings indicated that the best dose was P2 (1.5 mL/L), producing a total erythrocyte count of (291.40×10⁴ cells/mm³), hematocrit (37.66%), haemoglobin (9.43 g/dL), leukocyte count of (10.57×10⁴ cells/mm³), phagocytic activity of (32.00%), and survival rate of (92.33%). Water quality parameters remained within optimal ranges: temperature 26.8–28°C, pH 5.5–6.9, and dissolved oxygen (DO) 3.9–5.8 mg/L. These findings suggest that red dragon fruit peel extract at 1.5 ml/L has potential as a natural immunostimulant to prevent Aeromoniasis in P. hypophthalmus.
PELATIHAN KELOMPOK USAHA PASTEL MINI DESA PANDAU JAYA MELALUI DIVERSIFIKASI DAN FORTIFIKASI UDANG Jan Stepfely Simanjuntak; Mery Sukmiwati; Tjipto Leksono; Herdini Herdini; Henni Syawal; Afra Azizah; Andi Almer Farrand Noor; Faira Yovanie; Febi Nabila; Meisya Amanda Fazira; Mochammad Farkhan; Nabellia Nabellia; Putri Noviayu Salsabila Hendri; Rio Persal Anugrah
Jurnal Sinergitas PKM & CSR Vol. 6 No. 2 (2022): February
Publisher : Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19166/jspc.v6i2.5874

Abstract

Diversification and fortification are efforts that can be made in increasing the value of food quality in terms of increasing product varieties and adding product nutritional value through the addition of food ingredients, one example of this product is fish or shrimp. The purpose of this service is to provide training on CPPB-IRT, technology transfer for mini pastel product processing, labeling, mini pastel training SOPs, product packaging and label design, and website creation, Facebook, Instagram (information and promotions) as well as making fish/shrimp flavours on mini pastels. The method of implementation that would be carried out in this activity was in the form of education, with the method of lectures, discussions, demonstrations and evaluation of product processing/manufacturing techniques for mini pastel businesses. Targets and outcomes to be achieved from community service activities, namely mini pastel products sold to the market must meet product specifications in accordance with SNI 7388: 2009. Packaging and labeling were in accordance with food packaging standardization PP No. 69 of 1999, standard operating procedures for guarantees quality of mini pastels. Based on the results obtained, the responses from the owners and employees of the mini pastel business group were quite enthusiastic in participating in the counseling activities.abtract in bahasaDiversifikasi dan fortifikasi merupakan upaya yang dapat dilakukan dalam meningkatkan nilai mutu pangan dalam hal peningkatan varietas produk dan penambahan nilai gizi produk melalui penambahan bahan pangan, salah satu contoh produk ini adalah ikan atau udang. Tujuan pengabdian ini adalah memberikan pelatihan CPPB-IRT, alih teknologi pengolahan produk mini pastel, pelabelan, SOP pelatihan mini pastel, desain kemasan dan label produk, dan pembuatan website, facebook, instagram (informasi dan promosi) serta membuat rasa ikan/udang di mini pastel. Metode pelaksanaan yang akan dilakukan dalam kegiatan ini berupa edukasi, dengan metode ceramah, diskusi, demonstrasi dan evaluasi teknik pengolahan/pembuatan produk untuk usaha mini pastel. Sasaran dan hasil yang ingin dicapai dari kegiatan pengabdian kepada masyarakat yaitu produk mini pastel yang dijual ke pasaran harus memenuhi spesifikasi produk sesuai dengan SNI 7388 : 2009. Pengemasan dan pelabelan sudah sesuai dengan standarisasi kemasan pangan PP No. 69 Tahun 1999, standar operasional prosedur untuk menjamin kualitas mini pastel. Berdasarkan hasil yang diperoleh, respon dari pemilik dan karyawan cukup antusias mengikuti kegiatan penyuluhan.
Potential of Red Dragon Fruit (Hylocereus polyrhizus) to Prevent Aeromoniasis in Striped Catfish (Pangasianodon hypophthalmus) Tiara Oktari; Morina Riauwaty; Henni Syawal
Jurnal Perikanan dan Kelautan Vol. 31 No. 2 (2026): June
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.31.2.152-162

Abstract

Red dragon fruit peel is a natural material with antibacterial and antifungal properties, owing to its active compounds, including alkaloids, flavonoids, niacin, tannins, and vitamin C. These compounds can inhibit the growth of Aeromonas hydrophila and enhance fish immunity. This research was carried out from January to August 2025 at the Parasite and Fish Disease Laboratory, Faculty of Fisheries and Marine Sciences, Universitas Riau. This study aimed to examine the effectiveness of red dragon fruit peel solution (Hylocereus polyrhizus) in preventing Aeromoniasis in striped catfish (Pangasianodon hypophthalmus). The experiment used a Completely Randomized Design (CRD) with five treatments and three replications. Treatments consisted of KN: negative control (without immersion and not infected with A. hydrophila), KP: positive control (without immersion and infected with A. hydrophila), P1 (immersion at 1 ml/L and infected), P2 (immersion at 1.5 mL/L and infected), and P3 (immersion at 2 ml/L and infected). Fish were immersed in the peel solution for 30 minutes daily for three consecutive days. Blood sampling was conducted at the beginning of maintenance, on the 30th day, and on the 14th day after infection. The findings indicated that the best dose was P2 (1.5 mL/L), producing a total erythrocyte count of (291.40×10⁴ cells/mm³), hematocrit (37.66%), haemoglobin (9.43 g/dL), leukocyte count of (10.57×10⁴ cells/mm³), phagocytic activity of (32.00%), and survival rate of (92.33%). Water quality parameters remained within optimal ranges: temperature 26.8–28°C, pH 5.5–6.9, and dissolved oxygen (DO) 3.9–5.8 mg/L. These findings suggest that red dragon fruit peel extract at 1.5 ml/L has potential as a natural immunostimulant to prevent Aeromoniasis in P. hypophthalmus.
Physiological Responses and Nonspecific Immunity in Striped Catfish (Pangasianodon hypophthalmus) Fed Fermented Herbal Medicine Wiwik Yuliana; Henni Syawal; Morina Riauwaty; Fahrurrozi Fahrurrozi
Jurnal Perikanan dan Kelautan Vol. 31 No. 2 (2026): June
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.31.2.287-298

Abstract

Aeromonas hydrophila infections in aquaculture can cause mass mortality of up to 80–100% of striped catfish. Adding fermented herbal medicine containing essential oils, curcumin, flavonoids, and xanthorrhizol to feed can boost the fish's immunity. This study aims to analyze the optimal frequency of administering fermented herbal supplements in feed to enhance fish immunity, conducted from June 2024 to June 2025. A Completely Randomized Design (CRD) was used with 5 treatments and 3 replicates: Kn (no supplement, no infection), Kp (no supplement, infected with A. hydrophila), with 200 mL/kg of herbal supplement administered daily (P1), once every 3 days (P2), and once every 5 days (P3). The fish (P. hypophthalmus fingerlings, 8–12 cm, 20 fish/tank) were reared in 100-liter plastic buckets for 46 days. On day 32, the fish were infected with A. hydrophila (intramuscularly, 0.1 mL, 10⁸ CFU/mL), and they were maintained for 14 days thereafter. Each container was equipped with an aerator and a Dacron filter. The best results were obtained in P1, which showed a survival rate (SR) of 100%, a post-infection survival rate of 100%, a weight gain of 109.33 ± 2.51 g, serum protein of 3.94 ± 0.06 g/dL, albumin 3.76 ± 0.05 g/dL, globulin 0.21 ± 0.04 g/dL, lysozyme activity 373.33 ± 15.27 U/mL, and phagocytosis activity 51.11 ± 16.44%. Based on the data obtained, it can be concluded that the daily administration of 200 mL/kg of fermented herbal supplement in the diet yields the best results in enhancing the immune response and growth of P. hypophthalmus infected with A. hydrophila.
Physiological Responses and Nonspecific Immunity in Striped Catfish (Pangasianodon hypophthalmus) Fed Fermented Herbal Medicine Wiwik Yuliana; Henni Syawal; Morina Riauwaty; Fahrurrozi Fahrurrozi
Jurnal Perikanan dan Kelautan Vol. 31 No. 2 (2026): June
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.31.2.287-298

Abstract

Aeromonas hydrophila infections in aquaculture can cause mass mortality of up to 80–100% of striped catfish. Adding fermented herbal medicine containing essential oils, curcumin, flavonoids, and xanthorrhizol to feed can boost the fish's immunity. This study aims to analyze the optimal frequency of administering fermented herbal supplements in feed to enhance fish immunity, conducted from June 2024 to June 2025. A Completely Randomized Design (CRD) was used with 5 treatments and 3 replicates: Kn (no supplement, no infection), Kp (no supplement, infected with A. hydrophila), with 200 mL/kg of herbal supplement administered daily (P1), once every 3 days (P2), and once every 5 days (P3). The fish (P. hypophthalmus fingerlings, 8–12 cm, 20 fish/tank) were reared in 100-liter plastic buckets for 46 days. On day 32, the fish were infected with A. hydrophila (intramuscularly, 0.1 mL, 10⁸ CFU/mL), and they were maintained for 14 days thereafter. Each container was equipped with an aerator and a Dacron filter. The best results were obtained in P1, which showed a survival rate (SR) of 100%, a post-infection survival rate of 100%, a weight gain of 109.33 ± 2.51 g, serum protein of 3.94 ± 0.06 g/dL, albumin 3.76 ± 0.05 g/dL, globulin 0.21 ± 0.04 g/dL, lysozyme activity 373.33 ± 15.27 U/mL, and phagocytosis activity 51.11 ± 16.44%. Based on the data obtained, it can be concluded that the daily administration of 200 mL/kg of fermented herbal supplement in the diet yields the best results in enhancing the immune response and growth of P. hypophthalmus infected with A. hydrophila.
Physiological and Biochemical Responses in the Blood of Striped Catfish (Pangasianodon hypophthalmus) to the Addition of Papaya Seed Extract (Carica papaya L.) to Feed Dimas Gasliani Akbar; Henni Syawal; Morina Riauwaty
Jurnal Perikanan dan Kelautan Vol. 31 No. 1 (2026): February
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.31.1.17-24

Abstract

Striped catfish (Pangasianodon hypopthalmus) is a group of catfish that has become one of the leading freshwater commodities. This gives striped catfish (P. hypopthalmus) a fairly good market potential. A frequent constraint in fish farming is the risk of disease outbreaks. One factor contributing to the failure of fish farming and resulting in significant mortality is disease. One pathogenic bacterium responsible for disease is Aeromonas hydrophila, which is often found in freshwater and causes 80-100% of fish mortality in a short period. The method used is an experimental method employing a Completely Randomized Design (CRD) with one factor, 5 treatment levels, and 3 replications. The treatments used in this study refer to. The study on the addition of papaya seed extract (Carica papaya L.) to the feed found that it affects the physiological and biochemical responses of striped catfish (P. hypophthalmus). The best feeding dosage with the addition of papaya seed extract of 3.0 mL/kg of feed results in glucose levels of 70.33 mg/dL, calcium 12.53 mg/dL, magnesium 2.73 mg/dL, phosphorus 8.66 mg/dL, lysozyme activity 275 units/mL, absolute weight gain 14.92g, absolute length gains 5.25cm, and survival rate reaching 93.33%. The researcher recommends conducting further studies on the addition of papaya seed extract at a dosage of 3.0 mL/kg of feed to improve growth in different test fish and advises against using papaya seed extract in high doses.
Effect of Immersion of Striped Catfish (Pangasionodon hypophthalmus) in Red Dragon Fruit Peel Solution (Hylocereus polyrhizus) on Gill and Kidney Histopathology M. Taufik; Henni Syawal; Morina Riauwaty
Jurnal Perikanan dan Kelautan Vol. 31 No. 1 (2026): February
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.31.1.9-16

Abstract

Red dragon fruit peel is a natural material with antibacterial potential against fish diseases. This study was conducted from July 2024 to June 2025 at the Laboratory of Fish Parasites and Diseases, Faculty of Fisheries and Marine Sciences, Universitas Riau. The aim was to determine the histopathology of gills and kidneys in striped catfish (Pangasionodon hypophthalmus) immersed in red dragon fruit peel extract and challenged with Aeromonas hydrophila. The experiment used a Completely Randomized Design (CRD) with five treatments and three replications: Kn (negative control), Kp (positive control, challenged with A. hydrophila), P1 (1 mL/L), P2 (1.5 mL/L), and P3 (2 mL/L). Immersion was applied three times at the beginning of rearing, followed by 46 days of culture. A challenge test was carried out on day 32, and gill and kidney samples were collected on days 30 and 14 post-challenge. Results showed that fish challenged with A. hydrophila exhibited gill damage, including hypertrophy, lamellar curvature, telangiectasis, and epithelial lifting, as well as kidney damage, including necrosis, hemorrhage, and cell degeneration. The best result was observed in treatment P2 (1.5 mL/L), which effectively reduced tissue damage and improved the histological structure of gills and kidneys in striped catfish
Antibacterial Activity Test of Papaya Seed Extract (Carica papaya L.) on the Growth of Aeromonas hydrophila Lukman Basir; Morina Riauwaty; Henni Syawal
Jurnal Perikanan dan Kelautan Vol. 31 No. 1 (2026): February
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.31.1.47-54

Abstract

Aeromonas hydrophila is a pathogenic bacterium that can cause systemic disease and mass death. The disease caused by A. hydrophila bacteria is MAS (Motile Aeromonas Septicemia). One alternative treatment for A. hydrophila infection in fish is papaya seed extract (Carica papaya L.). This research was conducted from June 2024 to June 2025 at the Laboratory of Parasites and Fish Diseases, Aquaculture, Faculty of Fisheries and Marine, Universitas Riau. The purpose of this study was to determine the inhibition zone of papaya seed extract, as seen from the inhibition zone formed in inhibiting the growth of A. hydrophila, and also to determine the best MIC dose that can inhibit the growth of A. hydrophila. As well as to get a safe dose by conducting LD50 tests of papaya seed extract within 24 hours against common carp (Cyprinus carpio) with immersion techniques. The research method used was the experimental method, using the Kirby-Bauer disc method and a 6 mm diameter blank disk, with 3 repetitions per treatment. The results of the research indicate that papaya seed extract can inhibit the growth of A. hydrophila bacteria at concentrations of 100% - 1%, with an average inhibition zone diameter of 9, 86-6.2 mm. The MIC (Minimum Inhibition Concentration) of papaya seed extract inhibits the growth of A. hydrophila at 3.5%, with an average bacterial colony count of 280 CFU/mL. The LD50 value of papaya seed extract against common carp with the immersion technique for 24 hours is at a concentration of 3.73%.
Co-Authors Abdul Alif Abdul Alif Addilah, M. Fikri Adelin Adelina Adelina ' Afra Azizah Afrina Putri Afrizal Tanjung Alfian ' Alfinda, Rudi Alif, Abdul Amraini Fitri Anang Widarsa Andarini Diharmi Anderson.s, Roi Andesra, Andesra Andi Almer Farrand Noor Andre A Saragih, Andre A Andre Manusun Purba Anggriani, Rara Dewi Angraika Dirta Annisa Dwi Putri Anugrah, Rio Persal Anzila Rizki W Muharram Aris Pristiana Asmika Harnalin Simarmata Asri Mursawal Athif Amirah Elza Atika Talitha Erman Atria Martina Azizah, Afra Cerlina, Maya Chandra Chandra Chania Arnata Christin Natalia Cici Adliana Cindy Naomi Fadilla Christine Simalango Darfia, Novreta Dea Switzelin H Destriman Laoli Devy P Lumban Gaol Dian Fitria M Dian Iriani Dian Puspitowati Dicky Azwar Lubis Dimas Gasliani Akbar Dinar Mey Jasika H Diva, Farhah Dwi Indah Fransiska Elfira Stefani Priska Eni Sumiarsih Erikson Sahala Pardamean Etri Nopilita Fahrurrozi Fahrurrozi Faira Yovanie Fandi Rahmat Farkhan, Mochammad Farrand Noor, Andi Almer Fatihaturrahma, Annisya Fauzi Ahmad Lubis Faza, Farzana Fakhira Fazira, Meisya Amanda Febi Nabila Feri Julianto Fitria Sufriyani Fransiska, Dwi Indah Fricilia Zissalwa Gaol, Devy P Lumban Ginting, Kiki Dirgantara Gunawan B S Gusriansyah, Dimas Hafil Kurnia Tama Hasbullah Zakky Hasugian, Sampang Hotramadi Herdini , Herdini Herianti Rambe Heriyanti Heriyanti, Heriyanti Herparinda Novita Husna, Hidayatul Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukistyowati Iesje Lukstyowati Iesje Lukystiowati Ikhwanul Khairy Ilham Fajri Pohan Iman, Khasan Nur Ipa, Adha Irma Yunita Irsya, Habib Irwan Effendi Iskandar Putra Isma, Muhammad Fauzan Isna Sudirman Isna Sudirman Isnaini Lidya Rizki Jampil, Tirta Herlis Jan Stepfely Simanjuntak Jaya Maruli Tamba Juniarty Manurung Juwita Lestari Karnila, Rahman Karnila Khairia, Khairia Kiky Dirgantara Ginting Kurniawan, Ahmad Juli Kusai, Kusai Latifah Hannum Lase Lestari, Rona Lukman Basir Luksityowati, Iesje Luskityowati, Iesje M Riswan M Zaky Ditiya Putra M. Riswan M. Taufik M. Yanda, M. Yanda Manurung, Juniarty Mariana Lumban Tungkup Mariska, Refi Marwati ' Masjudi, Heri Maulina Sawitri Maya Cerlina Maytra Wirdona Meisya Amanda Fazira Melin Siburian Memi Martin Ilmayati Mery Sukmiwati Mesi Susanti Mochammad Farkhan Morina Riauwaty Muhamad Latiful Khobir Muhammad Akram Muhammad Arga Saputra Muhammad Arga Saputra Muhammad Arga Saputra Muhammad Azaldin Muhammad Iqbal Muhammad Muhajir Muhammad Muhajir, Muhammad Mulyadi . Mutawakkil, M. Mufid Al Mutiara Mutiara Nabellia Nabellia Nabellia, Nabellia Nabila, Febi Nasril Ismail NASTITI KUSUMORINI Nelli Karolina Sitorus Niken Ayu Pamukas Novike Zullanda Selvi, Novike Zullanda Novita, Herparinda Novreta Ersyi Darfia Nur Addini Nur Aini Nur Asiah Nur Ikhlas Syuhada Nuraini Nuraini Nuraini Nuraini Nuraini Nuraini Nurfitri, Maya Nurhasan Nurhasan Nurhasan Nurhasan, Nurhasan Oktavianti, Mila Pakpahan, Poltak Panggabean, Devi Yunita Pardamean, Erikson Sahala Pertiwi, Zuwini Poltak Pakpahan Poltak Pakpahan Purba, Andre Manusun Purba, Yoshua Dewaman Mangapul Putra, Mutlas Ade Putra, Sanjaya Mandala Putri Noviayu Salsabila Hendri Putri, Annisa Dwi Putri, Atika Putri, Mega Novia Putri, Rianti RA, Sayyidina Abdul Qabidhi Rabil Yusuf Rabil Yusuf Rahma Aprianti Rahmadona, Zikra Rahmat Iman Mainil Ramadhanita, Ramadhanita Rebeka Valensia Rhino Pamungkas Rianti Putri Riauwaty , Morina Ridho Aldiokta RIDWAN AFFANDI Rika Sinaga Rinaldi Rinaldi Rini Mastuti Rio Persal Anugrah Risman Ferdiansyah Riswan, M Riva Mutiara Rizka Hasanah Rizki Putra Rizvi, Fharisa Nabila Rona Lestari Ronal Kurniawan Roslina ' Rudi Alfinda Saberina Hasibuan Sagitha Aulya Fanny Tanjung Salsabila Hendri, Putri Noviayu Santa, Santa Santikawati, Susi Sarah Pabiola Saragih Siallagan, Romensius Anggi AW Siburian, Melin Sihaloho, Rosalinda Simanjuntak, Jan Stepfely Sinaga, Tomuso Angdi Suryani Siregar, Cici YP Siregar, Desty Sahroni Siregar, Morina Riauwaty Sri Wahyuni Sri Wahyuni Suci Wulandari Sudirman, Isna Syafriadiman, Syafriadiman Syafruddin Nasution Syahputra, Mohamad Ardani Syahrul Hidayat Syaieba, Mutiara Tamba, Jaya Maruli Tanjung, Yuliza Virliansyah Telaumbanua, Siti Tia Suherianti Tiara Oktari Tjipto Leksono Tjipto Leksono Tomuso Angdi Suryani Sinaga Trisla Warningsih Tungkup, Mariana Lumban Ulandari, Ulfa Unggul Fitrah Heriadi Usman M Tang Usman M Tang Usman M Tang Usman Muhammad Tang Vekri Ramadhi Wasmen Manalu Widarsa, Anang Wijaya, Rahmat Santosa Windarti Windarti Wiwik Yuliana Yetti Elfina Yetti Elfina S Yoshua Demawan Mangapul Purba Yosri Yosri Yovanie, Faira Yuharmen - Yuliati Yuliati Yulyawati Yulyawati Yunita Paramitha Hasibuan Yusni Ikhwan Yusni Ikhwan Siregar Yusuf, Rabil Zebua, Ratna Dewi Zikra Rahmadona Zissalwa, Fricilia Zulaiha ' Zulkarnain Zulkarnain Zulkarnain Zulkarnain