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All Journal Jurnal Sain Veteriner Microbiology Indonesia Media Kedokteran Hewan Indonesian Journal of Tropical and Infectious Disease Masyarakat, Kebudayaan dan Politik Jurnal Medik Veteriner Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Journal of Applied Veterinary Science and Technology journal of Basic Medical Veterinary Agrivet: Jurnal Ilmu-ilmu Pertanian dan Peternakan Journal of Pharmaceutical and Sciences. Journal of Fisheries & Marine
Aksono HP., Eduardus Bimo
Division Of Veterinary Basic Medicine, Faculty Of Veterinary Medicine, Universitas Airlangga, Surabaya-Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Addition of basil leaf (Ocimum basicilum L.) extract to AndroMed® extender improved the quality of spermatozoa of frozen thawed Simmental bull semen Aditya Irawan; Fajar Ramadhan Subiyantoro; Suherni Susilowati; Tri Wahyu Suprayogi; Sri Pantja Madyawati; Pudji Srianto; Nove Hidajati; Eduardus Bimo Aksono Herupradoto; Kadek Rachmawati
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 3 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i3.2024.161-170

Abstract

This study aims to determine the best dose of basil leaf extract to add to AndroMed® semen extender to improve spermatozoa quality after freezing and thawing. This study used healthy, normal four-year-old Simmental bulls with good libido. The sample used was fresh semen from Simmental bull collected using an artificial vagina. This study used a completely randomized design (CRD) with three treatments and six replicates. Ejaculate that met the motility qualification of more than 70%, was divided into three groups, each extended in AndroMed® without the addition of basil leaf extract (T0), with the addition of 0.1% basil leaf extract (T1) and with the addition of 0.3% basil leaf extract (T2). Straw containing semen with 30 million spermatozoa/straw was processed using an IceCube automatic freezer (Minitube, Germany). The results showed that spermatozoa motility, viability, and spermatozoa plasma membrane integrity in the T2 group were higher (p <0.05) than those in the T0 and T1 groups, while spermatozoa morphological abnormalities in the T2 group were lower (p <0.05) than those in the T0 and T1 groups. There were no significant differences (p >0.05) in these parameters between groups T0 and T1. It could be concluded that the addition of 0.3% basil leaf extract to the AndroMed® extender could improve the quality of the semen of Simmental bulls after thawing.
Efficacy of Ethanol Extract of Papaya Leaves (Carica papaya var California L.) on Mortality of Instar III Larvae of Aedes aegypti Mosquito Choirul Oktavian Setiyadin Nayiron; Hastutiek, Poedji; Dewa Ketut Meles; Eduardus Bimo Aksono Herupradoto; Mochamad Lazuardi; Agus Sunarso
Journal of Parasite Science Vol. 9 No. 2 (2025): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v9i2.64877

Abstract

This study aims to evaluate the efficacy of Papaya var California leaves extract and identify its effective concentration in inducing mortality among third-instar larvae of Aedes aegypti mosquitoes. The study utilized adult Ae. aegypti mosquitoes as the experimental subjects. This study was an experimental study with a comparative Completely Randomized Design The test groups include negative control, 2.5 mg/mL, 5 mg/mL, 10 mg/mL and positive control. Each test used 20 third instar larvae of  Ae.aegypti mosquitoes, with four repeats..  The test groups were 2.5 mg/mL, 5 mg/mL, 10 mg/mL, and the control groups were A negative control contained distilled water and Tween 20, and a positive control contained abate (temephos) Based on Kruskall-wallis test shows a significant difference. The results indicated that the optimal concentration was 10 mg/mL, with the highest effectiveness observed at the 30th time hours.
The Sex Identification of the Sun Conure (Aratinga solstitialis) Using Calamus Based on Polymerase Chain Reaction Pinayungan, Probo Probo Warih Tatag; Aprinda Ratna Lovela; Eduardus Bimo Aksono; Ismudiono; Boedi Setiawan; Kadek Rachmawati; Jola Rahmahani
Media Kedokteran Hewan Vol. 35 No. 1 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i1.2024.21-27

Abstract

Sex identification of Sun Conures (Aratinga solstitialis) is crucial for breeding and preservation, as well as increasing sun conure populations. These birds are sexually monomorphic. Therefore, Determination between male and female carried out by their morphology examination. The Polymerase Chain Reaction (PCR) method, utilizing molecular-based technology, was employed to determine the sex of Aratinga solstitialis in this study. The P2 and P8 primers were utilized in this method, which has been deemed suitable and accurate for sex identification through calamus samples. The research focused on two 28-month-old Aratinga solstitialis birds. Calamus samples were collected and subjected to PCR amplification using the extracted calamus. The resulting PCR products were then visualized using electrophoresis with a 1% agarose gel. In the electrophoresis photo, the presence of two bands indicated a female specimen, whereas a single band indicated a male specimen. The result of the gel electrophoresis research showed that both of the Aratinga solstitialis were male with one band of each bird on ranged from 300-400 base pairs. The result show that the Polymerase Chain Reaction method in terms for sex identification on monomorphic birds, especially Aratinga solstitialis birds is very effective to differentiate the sex of young birds and the adults.
Designing of a Novel Aerolysin-based Multiepitope Vaccine against Aeromonas hydrophila Isolated from Osphronemus goramy Using Reverse Vaccinology: an in Silico Approaches Rozi; Tyasningsih, Wiwiek; Rahmahani, Jola; Aksono, Eduardus Bimo; Yunus , Muchammad; Al-Arif, Mohammad Anam; Kuncorojati, Suryo; Kusdarwati, Rahayu; Sari, Putri Desi Wulan; Amal, Mohammad Noor Azmai; Salleh, Annas; Khanand, Nadeem; Suwarno
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 2 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i2.62035

Abstract

Graphical Abstract Highlight Research The study aims to develop a multi-epitope vaccine (MEV) against A. hydrophila by targeting the aerolysin toxin, a key virulence factor responsible for infections in fish and humans. Computational methods identified and optimized B-cell and T-cell epitopes, focusing on their ability to trigger immune responses without causing toxicity or allergenicity. In silico simulations demonstrated that the MEV has a strong binding affinity to immune receptors like TLR-4, MHC-I, and MHC-II, indicating its potential to induce robust cellular and humoral immunity. Structural analysis of the MEV showed a stable 3D conformation, with most residues in favorable regions, ensuring stability during immune activation. The MEV could enhance disease control in aquaculture and reduce human infection risks, offering a promising solution to address antibiotic resistance and the absence of effective vaccines. Abstract Aeromonas hydrophila, gram-negative, is a major pathogen responsible for various diseases in mammals, reptiles, amphibia, and vertebrates, including fish and humans. Targeting the specific toxin aerolysin in A. hydrophila is crucial to address antibiotic resistance and the lack of adequate and protective vaccines against this intracellular pathogen. This study aimed to identify a multi-epitope vaccination (MEV) candidate targeting A. hydrophila aerolysin toxin to combat the disease effectively. Standard biochemical characterization methods and sequencing of the 16S rRNA, rpoB, and aerA genes identified the isolate AHSA1 as A. hydrophila. Subsequently, we identified B and T cell epitopes on the aerolysin protein and separately predicted MHC-I and MHC-II epitopes. The epitopes are then evaluated for toxicity, antigenicity, allergenicity, and solubility. The vaccine design integrated multi-epitope-based constructs, utilizing specialized linkers (GPGPG) and EAAAK linkers to connect epitope peptides with adjuvants in the cholera toxin B component, thereby enhancing immunogenicity. Ramachandran plots showed that 85.25% of the residues were located in the most favorable regions, which was followed by the generously allowed zone (1.30%), the additional allowed regions (10.80%), and the forbidden regions (2.65%), thus confirming the feasibility of the modeled vaccine design. Based on docking simulations, MEV had the highest binding and interaction energies with TLR-4, TLR-9, MHC-I, and MHC-II (-1081.4, -723.2, 866.2, -9043.3 kcal/mol). Based on computational modelling, we expect the Aerolysin MEV candidate design to activate diverse immune mechanisms, stimulate robust responses against A. hydrophila, and maintain safety. The significant solubility, absence of toxicity or allergic response, and minimal side effects in animal testing all contribute to the potential clinical utility of this vaccine candidate.  
Red dragon (Hylocereus polyrhizus) fruit peel extract increased the motility and viability of spermatozoa of hypercholesterolemic rats (Rattus norvegicus) Niken Meyliana Sari; M. Gandul Atik Yuliani; Eduardus Bimo Aksono Herupradoto; Nanik Hidayatik; Nurhusien Yimer; Tatik Hernawati
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 1 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i1.2023.34-42

Abstract

This study aims to determine the effect of red dragon (Hylocereus polyrhizus) fruit peel extract (RDFPE) on spermatozoa motility and viability of hypercholesterolemic rats (Rattus norvegicus) as a model. Twenty male rats were randomly divided into negative control (NC), positive control (PC), treatment 1 (T1), treatment 2 (T2), and treatment 3 (T3) groups. All rats were given 2 mL of high cholesterol feed orally every day for 28 days. On day-15, all rats were measured for their blood cholesterol levels, followed by treatment for 14 days. Rats in the NC, PC, T1, T2, and T3 groups were treated with 1% Na-CMC, Simvastatin 10 mg/kg BW, and RDFPE of 500, 750, and 1000 mg/kg BW, respectively. On day-29, all rats were sacrificed to evaluate spermatozoa viability and motility. The results showed that spermatozoa viability and motility in the hypercholesterolemic rats (NC) group were the lowest (p <0.05) among the groups. Treatment of hypercholesterolemic rats with Simvastatin 10 mg/kg BW (PC) group showed higher (p <0.05) spermatozoa viability and motility compared to the NC group. RDFPE dose of 1000 mg/kg BW (T3 group) resulted in higher (p <0.05) spermatozoa viability and motility compared to other RDFPE doses (T1 and T2) and the control (NC) groups, and it was similar (p >0.05) compared with the Simvastatin treated (PC) group. It could be concluded that the administration of 1000 mg/kg BW ethanolic extract of red dragon fruit (Hylocereus polyrhizus) peel increased the viability and motility of spermatozoa of hypercholesterolemic rats (Rattus norvegicus) which were the same as the group of rats given Simvastatin 10 mg/kg BW.
Penilaian densitas kolagen pada luka bakar derajat II tikus putih (Rattus norvegicus) yang diterapi dengan krim ekstrak daun melinjo (Gnetum gnemon L.) Srimaryanto, Leonardo Reza; Hidajati , Nove; Triakoso , Nusdianto; Herupradoto, Eduardus Bimo Aksono; Lazuardi, Mochamad; Setiawan, Boedi
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 1 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i1.680

Abstract

Second-degree burns are complex traumatic conditions requiring optimal tissue healing interventions. This experimental study investigated the impact of Gnetum gnemon L. leaf extract cream on collagen density in burn wounds. The research was conducted using 20 male Wistar rats (Rattus norvegicus), divided into five groups: negative control, positive control (silver sulfadiazine), and three treatment groups with varying leaf extract concentrations (2.5%, 5%, and 10%). Burn induction was performed on the right gluteal area using a thermostat at 85°C for 5 seconds. Collagen density was evaluated through histopathological analysis using ImageJ with density scores ranging from 0 to 4. Research results demonstrated significant collagen density increases in treatment groups, with the 10% concentration displaying the highest density (score +4). Significant differences between groups were confirmed by statistical analysis (p<0.05). Findings indicate the potential of Gnetum gnemon L. extract as an alternative therapy for improving burn wound healing through collagen density optimization.
Detection of Vancomycin-Resistant Enterococci (VRE) Genes in Imported Canary (Serinus canaria) Faeces Dina Agylia Rahmandari; Mustofa Helmi Effendi; Yulianna Puspitasari; Eduardus Bimo Aksono Herupradoto; Lilik Maslachah; Boedi Setiawan; Fifin Kurnia Sari; Tri Endah Purbowati; Izzatul Istiana
Journal of Basic Medical Veterinary Vol. 15 No. 1 (2026): Journal of Basic Medical Veterinary, June 2026
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v15i1.83728

Abstract

Antibiotic-resistant Enterococcus is currently one of the leading causes of nosocomial infections worldwide. Multidrug-resistant (MDR) Enterococcus is an important nosocomial pathogen and represents a growing clinical challenge. This study aimed to determine the presence and characteristics of Vancomycin-Resistant Enterococci (VRE) genes in the faeces of imported canaries (Serinus canaria) to assess their potential impact on animal health and the spread of antimicrobial resistance. A total of 150 canary faecal samples were randomly collected from a single importer. Colonies with Enterococcus-like morphology were identified at the genus level based on culture characteristics, Gram staining, catalase testing, and bile-esculin testing. Species identification was confirmed through carbohydrate fermentation tests. Antibiotic susceptibility testing was performed using the Kirby–Bauer method on Mueller–Hinton agar. Polymerase chain reaction (PCR) testing was subsequently conducted to identify the vanB gene. Among the samples, 39 (26%) were positive for Enterococcus faecalis. Of these isolates, 7 (17.9%) were identified as multidrug-resistant (MDR), and 5 (71.4% of MDR isolates) were classified as VRE. One of the five VRE isolates (20%) was found to carry the vanB gene, an important determinant of vancomycin resistance. These findings indicate that imported canaries may serve as reservoirs of antimicrobial-resistant bacteria with the potential to spread to the environment, other animals, and humans through the faecal–oral route or environmental contamination.
Analisis kelayakan usaha dan strategi pengembangan usaha peternakan lebah Trigona sp. Desa Pangkal Niur Kabupaten Bangka Elzam; Anwar Ma’ruf; Emy Koestanty Sabdoningrum; Eduardus Bimo Aksono Herupradoto; Dadik Raharjo; M. Gandul Atik Yuliani
Agrivet : Jurnal Ilmu-Ilmu Pertanian dan Peternakan (Journal of Agricultural Sciences and Veteriner) Vol. 13 No. 2 (2025): Desember
Publisher : Fakultas Pertanian Universitas Majalengka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31949/agrivet.v13i2.15909

Abstract

This study aims to analyze the feasibility of Trigona sp. beekeeping as an alternative sustainable livelihood, identify internal and external faktors influencing business development, and formulate strategies as a basis for recommendations to beekeepers and stakeholders in ensuring sustainable growth and economic value. The research employed a survey approach using questionnaires, semi-structured interviews, and field observations, as well as a quantitative method focusing on financial analysis through the calculation of investment costs, operational costs, revenues, and feasibility indicators such as BEP, NPV, IRR, BCR, and Payback Period. Internal and external faktors were analyzed using the IFE, EFE, and IE Matrices, while development strategies were formulated through the SWOT Matrix involving 15 respondents with at least five years of beekeeping experience. The results indicate that Trigona sp. beekeeping is financially profitable and feasible to develop, as reflected by the low BEP of 44 kg/IDR 3,534,852, a positive NPV of IDR 138,067,768, an IRR of 238%, a BCR of 9.2, and a Payback Period of 0.5 (6 months). Recommended development strategies include product diversification, cultivation of feed sources, capacity building of beekeepers through training, regular product quality testing, strengthening distribution through partnerships, effective pest control, and the implementation of colony maintenance SOPs to sustain productivity and honey quality. Based on the findings, it can be concluded that Trigona sp. beekeeping is feasible and profitable to develop, supported by development strategies that emphasize product diversification, capacity enhancement, quality and distribution strengthening, as well as sustainable maintenance management to ensure economic value and long-term business sustainability.
Co-Authors Adelina Grace Aditya Irawan Ahmad Thoriqul Firdaus Al arif, Mohammad Anam Al-Arief, Mohamad Anam Aldise Mareta Nastri, Aldise Mareta Amal, Mohammad Noor Azmai Ana Adelina Farahdiba Anwar Ma'ruf Anwar Ma'ruf Anwar Ma’ruf Aprinda Ratna Lovela Aquaresta, Febriana Arianto, Intan Diah Safitri Aryaloka, Suhita Aryati Aryati Ayuti, Siti Rani Bambang Sektiari Lukiswanto Billa, Lutfiah Annisa Boedi Setiawan BUDI UTOMO Candra Dwi Atma Choirul Oktavian Setiyadin Nayiron Chusniati, Sri Dadik Raharjo Dina Agylia Rahmandari Dyah Ajeng Suhita Eka Pramyrtha Hestianah Elzam Emy Koestanty Sabdoningrum Endang Suprihati Erlita Rusnaningtias Fajar Ramadhan Subiyantoro Fedik Abdul Rantam Fifin Kurnia Sari Firnanda, Ferian Gandul Atik Yuliani HAK HOTTA Hapsari, Nafisah Nurul Hasib, Abdullah Heni Puspitasari HERNOMO ONTOSENO KUSUMOBROTO Hestiana, Eka Pramyrtha Hidayatik, Nanik Ira Sari Yudaniayanti Ira Sari Yudaniyanti Ismudiono Iwan Sahrial Hamid Izzatul Istiana Juniastuti Juniastuti Kadek Rachmawati Khairullah, Aswin Rafif Khanand, Nadeem Kuncorojati, Suryo Kurniawan, Muhammad Ridho Hafid Kusnoto Kusnoto Lastoaji, Firman Lilik Maslachah Lilis Mundri Jannah, Lilis Mundri Lovela, Aprinda Ratna Lucia Tri Suwanti, Lucia Tri M. Gandul Atik Yuliani Maria Inge Lusida MARIA INGE LUSIDA Meles, Dewa Ketut Mirni Lamid Mochamad Lazuardi Moses, Ikechukwu Benjamin Mufasirin Muhammad Qushai Y. Matondang, Muhammad Qushai Y. Muhammad Rivai Mustofa Helmi Effendi Nanik Hidayatik Nasronudin Nasronudin Niken Meyliana Sari Nove Hidajati Nufus, Faizah Zakiyyatun Nur Syamsiatul Fajar, Nur Syamsiatul Nurfitri, Yulia Nurhusien Yimer Nurrahmad, Nanda Rino Nusdianto Triakoso Pinayungan, Probo Probo Warih Tatag Poedji Hastutiek Pudji Srianto Puput, Sesa Rahaju Ernawati Rahmahani, Jola Rahmawati, Kadek Rizka Fathoni Perdana Rochmah Kurnijasanti Rozi Salleh, Annas Sari, Aulia Puspita Sari, Putri Desi Wulan Sasmono, R. Tedjo Setiawan Koesdarto Shifa Fauziyah Sin War Naw SOETJIPTO . Sri Herawati Sri Pantja Madyawati Sri Subekti Srimaryanto, Leonardo Reza Sudar Itafarida Suherni Susilowati Sukmanadi, Mohammad Sunarso, Agus Suroso, Bakti Suwarno TAKAKO UTSUMI Tatik Hernawati Teguh Hari Sucipto, Teguh Hari Tita Damayanti Lestari Tri Endah Purbowati Tri Wahyu Suprayogi Tyasningsih, Wiwiek Usma Nur Dian Rosyidah Wahyuni, Retno Sri Widiyatno, Thomas Valentinus Widya, Alicia Margaretta Wiwik Misaco Yuniarti Yohan, Benediktus YOSHIHIKO YANO YOSHITAKE HAYASHI Yuliani, Margareth Gandul Atik Yulianna Puspitasari Yunus , Muchammad Yunus, Muchammad