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Journal : Tropical Animal Science Journal

Optimization of Phytase Production from Rhodotorula mucilaginosa RG-PK20 Using Agricultural Waste Seprianto; Utama, C. C. Y.; Melani, V.; Handayani, P.; Sukarman
Tropical Animal Science Journal Vol. 48 No. 4 (2025): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2025.48.4.328

Abstract

Some ingredients in poultry feed contain phytic acid, which prevents the absorption of nutrients. Microbial phytase enzymes can help with this problem. However, the phytase-producing gene of the novel yeast Rhodotorula mucilaginosa RG-PK20 has been constrained by the high cost of traditional substrates such as sodium phytate. The aim of this study is to evaluate the production of phytase from R. mucilaginosa RG-PK20 using a phytic acid source (substrate) from agricultural waste, with in vitro testing in poultry feed. The fermentation process was conducted utilizing a substrate-to-medium-to-yeast culture ratio of 1:1:1 v/v/w over a period of four days at various temperatures (25, 28, and 30 °C) and pH levels (3, 4, 5, and 6, with 7 as a control). Glucose and urea supplements were given when the optimal conditions were established by measuring the phytase content and activity. The molecular weight of the phytase was confirmed by SDS-PAGE analysis, and the ability of the enzyme to hydrolyze phytic acid was evaluated in vitro. Corn cobs generated the highest amount of phytase, with a concentration of 25.29 mg/mL and activity of 4.46 U/mL. The in vitro tests revealed an 81% reduction in phytic acid levels in poultry feed. These results demonstrate the potential of phytase derived from R. mucilaginosa RG-PK20 to reduce phytic acid in poultry feed ingredients.
Co-Authors Abdullah, Amri Achdar HT, M. Nur Rahmat Adi Suriatno Afgani, Abduh Al Afif Hakim Afriani, Ina Agus Supriyanto Agustini Ahmad Muslim Akhmad Junaedi Al Hadana, Farid Alamul Huda Aliva Rosdiana Amar Amir Amir Amir Amri Abdulah Andhega Wijaya Andi Indianto Andriyanto, Octo Dendy Apreti, Tiara Aprizal Ardi Rajab, Dede Arief, Nur Ahmad Aryadi, Fadli Rifki Asrori Awalia, Haifazah Burdi, Siti Adkha Mufroh Dena Meliana Didik Nurhadi Djunaedi Dodi Mulyadi Erna Fitriatun Fachrizal Ahmad Setiawan Fahri, Marjana Fatmawati Sabur Fatri Ardiansyah Firamulia, Muhammad Tryas Budi Fitri Roihani Fitriatun, Erna Halimatus Sa'diyah Hananto, Agustia Handayani, P. Hardika, Meilita Hesti Sadtyadi Ihfan Rahmawadi Ilmi, Uswatul Imam Mandriyanto Imam Sofi’i Imroatul Rofiah Indri Susilawati Karyadi Khalik, Idhan Khalimatus Sa’diyah Khoiroh, Nida'ul Khoirudin Khoirudin Khoirudin, Khoirudin KHOIRUL ANWAR Ladun Hakim Lalu Khairul Hadi Lintang Azizah, Renata Lusi Hildawati Luthfan Fajriansyah Ma'mulah Noor Mael, Masilva Raynox Mahalli Margono, Farhan Masriah Maulana, Miqdam Melani, V. Midranisiah Muchammad Chusnan Aprianto Muhamad Abdika Mafruh Muhammad Najih Murtalim Nana Rahdiana Nur Alam, Deden Nurdin Nurmala Fadhilah Jaya Panich, Pensri Pratama, Tito Chaerul Pusaka, Semerdanta Rahdiana, Nana Railia Karneta Rhubido, Dadang Riska Aini Rizka Dwi Alfinda Rizki Aulia Nanda Rohmah Roy Waluyo Rusdiana Yusuf Ryan Anggriawan Sabita, Lutfiah Saiful Anwar Saipul Hamdi Salabi Salamah, Wichda Ainis Saniman Saputra Sigalingging, Wanri Sarilah Seprianto Septiana, Hespi Shintia Siti Nur Suhaeni Siti Umiyati Sri Erny Muliyani Subaidi Sugiarto, Vanesa Oktaviana Sugiharto Suhara, Ade Sujiono Suripto, Heri Susanto, Indri Tahir, Arsil Taufik Ulhakim, Muhamad Taufik Ulhakim, Muhammad Teknik Mesin Tiara Shiffany Tikamori, Ghazi Toto Supriyono, Toto Tri Oktaviani Ucok Ummi Sholikhah Utama, C. C. Y. Vientiani, Cynthia Yanuarini, Erlina Yose Rizal Yulia Rohmatin Yusuf Nur Ubaidillah Zaidar, Chilfi Zuhriyah Zulkarnain, Rofik