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Karakterisasi, Skrining dan Uji Aktivitas Antibakteri Ekstrak Etanol Bunga Kecombrang (Etlingera elatior (Jack) R.M.Sm) terhadap Staphylococcus aureus Khairunnisa , Ardina; Yuniarti, Rafita; Dalimunthe, Gabena Indrayani; Rani, Zulmai
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 2 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i2.870

Abstract

Background: Torch ginger (Etlingera elatior (Jack) R.M.Sm) is a traditional medicinal plant with potential antibacterial properties due to its secondary metabolites, such as flavonoids, tannins, and terpenoids. Staphylococcus aureus is a pathogenic bacterium causing various infections, and increasing antibiotic resistance necessitates alternative treatments. This study aims to explore the antibacterial potential of torch ginger flower ethanol extract against S. aureus. Objective: This research aimed to characterize the phytochemical content of torch ginger flowers, screen for bioactive compounds, and evaluate their antibacterial activity against S. aureus. Methods: The study employed an experimental design, including phytochemical screening, ethanol extraction (maceration), and antibacterial testing using the disk diffusion method at concentrations of 10%, 30%, 50%, 70%, and 90%. Data were analyzed using one-way ANOVA. Results: Phytochemical screening revealed the presence of alkaloids, flavonoids, tannins, saponins, steroids/triterpenoids, and glycosides. The ethanol extract exhibited antibacterial activity against S. aureus, with inhibition zones of 11.9 mm (weak) at 10%, 16.1 mm (moderate) at 30%, and 21.3–22.5 mm (strong) at 50–90%. Statistical analysis confirmed significant differences (p < 0.05) between concentrations. Conclusion: Ethanol extract of torch ginger flowers demonstrated dose-dependent antibacterial activity against S. aureus, supporting its potential as a natural antibacterial agent.
Kandungan Vitamin C dan Uji Aktivitas Antioksidan Teh Herbal Kombucha Daun Bidara (Ziziphus mauritiana Lam) Siagian , Anggi Yani; Yuniarti, Rafita; Daulay, Anny Sartika; Pulungan , Ainil Fithri
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 2 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i2.880

Abstract

Background: Bidara leaves (Ziziphus mauritiana Lam.) have long been used in traditional medicine due to their bioactive compounds, such as flavonoids, tannins, and vitamin C. Fermenting bidara leaves into kombucha has the potential to enhance antioxidant activity through bioconversion processes carried out by starter microbes (Acetobacter xylinum and Saccharomyces cerevisiae). However, scientific data regarding the vitamin C profile and antioxidant activity of bidara leaf kombucha remain limited. Objective: This study aims to analyze the vitamin C content and antioxidant activity of bidara leaf herbal kombucha, as well as to characterize the quality of the simplisia based on the Indonesian National Standard (SNI) 4324:2014. Methods: This descriptive study involved: (1) preparation of bidara leaf simplisia, (2) 12-day kombucha fermentation, (3) phytochemical screening, (4) determination of vitamin C content using UV-Vis spectrophotometry (λ = 266 nm), and (5) antioxidant activity assay using the DPPH method (λ = 517 nm). Results: The bidara leaf simplisia met the SNI quality standards, with a moisture content of 5.33% and a total ash content of 6.73%. Phytochemical screening identified the presence of flavonoids, saponins, tannins, glycosides, steroids/triterpenoids, and alkaloids. The vitamin C content of the kombucha was 0.009 mg/g, while the IC₅₀ value for antioxidant activity was 621.2158 µg/mL, which falls into the weak category. Conclusion: Bidara leaf kombucha contains bioactive compounds and vitamin C, with weak antioxidant activity. Fermentation increases antioxidant activity compared to non-fermented bidara tea infusion (IC₅₀ = 1129.0069 µg/mL), although it remains lower than that of pure vitamin C (IC₅₀ = 3.0710 µg/mL).
Uji Antibakteri Ekstrak Etanol Daun Mangga Golek (Mangifera indica L.) terhadap Bakteri Staphylococcus aureus: Antibacterial Test of Ethanol Extract of Golek Mango Leaves (Mangifera indica L.) Against Staphylococcus aureus Bacteria Yuniarti, Rafita; Rukmana, Siti; Nasution, Haris Munandar; Rani, Zulmai; Fauzi, Ziza Aisyia Putri
Jurnal Sains dan Kesehatan Vol. 7 No. 2 (2025): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v7i2.2427

Abstract

Mango golek is among the numerous beneficial plants in the Mangifera genus. Mango leaves have antibacterial, antifungal, antiviral, and antiparasitic properties. The purpose of this research was to determine how effective an ethanol extract of mango golek leaves was against Staphylococcus aureus bacteria and what kind of secondary metabolites were present in the extract. This study employed laboratory experimental methods such as the maceration method with 70% ethanol solvent, phytochemical screening test, and Mango golek leaf extract (Mangifera indica L.) Staphylococcus aureus antimicrobial activity test at various concentrations of 100 mg/day. mL, 70, 50, 30, and 10 mg/mL using the agar diffusion method. Mango golek leaves ethanol extract included alkaloids, flavonoids, saponins, tannins, and steroids/triterpenoids, according to the screening results. values of 100 mg/mL and 70 mg/mL were considered high, while values of 50 and 30 mg/mL were considered moderate. Keywords:          cMangifera indica L., Staphylococcus aureus   Abstrak Salah satu tanaman dari genus Mangifera adalah mangga golek yang memiliki banyak manfaat. Salah satu keuntungan adalah sifat antiparasit, antijamur, antivirus, dan antibakterinya. Tujuan penelitian ini adalah untuk mengetahui seberapa efektif ekstrak daun mangga golek terhadap Bakteri Staphylococcus aureus dan mengidentifikasi metabolit sekundernya. Dalam penelitian ini, menggunakan metode eksperimental yang meliputi uji fitokimia, pembuatan pelarut etanol 70% dari ekstrak daun mangga golek (Mangifera indica L.), dan uji antibakteri Staphylococcus aureus dengan konsentrasi 100, 70, 50, 30, dan 10 mg/mL menggunakan metode difusi agar. Hasil skrining, ekstrak etanol daun mangga golek mengandung alkaloid, flavonoid, saponin, tanin, dan steroid/triterpenoid. Uji antibakteri, ekstrak daun mangga golek memiliki sifat antimikroba Staphylococcus aureus dengan kategori kuat 100 dan 70 mg/mL dan kategori sedang 50 dan 30 mg/mL. Kata Kunci:         Antibakteri, Mangifera indica L, Staphylococcus aureus
Antibacterial Activity Test of Ethanol, N-Hexane, and Ethyl Acetate Extracts of Lemon Peel (Citrus x limon (L.) Burm. f.) Nisa, Uswatun; Mambang, D Elysa Putri; Yuniarti, Rafita; Ridwanto, Ridwanto
Indonesian Journal of Science and Pharmacy Vol. 2 No. 3 (2025): Indonesian Journal of Science and Pharmacy
Publisher : Pustaka Media Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63763/ijsp.v2i3.88

Abstract

Staphylococcus hominis and Pseudomonas aeruginosa bacteria are normal flora bacteria found on the surface of human skin, and in certain circumstances can cause infections in the body of a person with a weak immune system. Infection of both bacteria can cause body odor that can affect a person's social relationships. The use of natural antibacterials has minimal side effects, namely lemon peel which is waste from lemons. Lemon juice is commonly used by the public, but not many people know that lemon peel has great benefits like lemon juice. The objective of the research was to determine the chemical compounds contained in the n-hexane fraction, ethyl acetate of lemon peel and antibacterial activity in ethanol extract, n-hexane fraction, ethyl acetate of lemon peel (Citrus x limon (L.) Burm. f.) This research method was an experimental method that includes sample collection and processing, characterization of simple drugs, preparation of 96% ethanol extract, n-hexane fraction, ethyl acetate fraction, phytochemical screening, and antibacterial activity test using the agar diffusion method at concentrations of 10%, 20%, 30%, 40%, 50%. The results of phytochemical screening in the ethyl acetate fraction contain alkaloids, flavonoids, tannins, triterpenoids, and in the n-hexane fraction shows the presence of triterpenoid chemical compounds. Ethanol extract, n-hexane fraction and ethyl acetate fraction of lemon peel have antibacterial activity on Staphylococcus hominis and Pseudomonas aeruginosa bacteria. The results of the three samples tested for the greatest inhibitory power in the ethyl acetate fraction showed intermediate results, almost comparable to the inhibitory power of the positive control ciprofloxacin.
Evaluation of Butterfly Pea (Clitoria ternatea L) Extract Gel as an Antibacterial Agent Against Staphylococcus epidermidis Asia, Nur; Lubis, Minda Sari; Yuniarti, Rafita; Dalimunthe, Gabena Indrayani
Indonesian Journal of Science and Pharmacy Vol. 2 No. 3 (2025): Indonesian Journal of Science and Pharmacy
Publisher : Pustaka Media Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63763/ijsp.v2i3.89

Abstract

Acne vulgaris is a common dermatological condition, particularly prevalent among adolescents and young adults. This condition is influenced by several factors, including excessive sebum production, abnormal keratinization, inflammation, and colonization of microorganisms such as Staphylococcus epidermidis within the pilosebaceous follicles. This study aims to develop a gel formulation containing butterfly pea (Clitoria ternatea) extract that meets the physical quality standards of the Indonesian National Standard (SNI) and exhibits antibacterial activity against Staphylococcus epidermidis. The research involved purposive sampling of butterfly pea flowers from Medan City, followed by extraction and phytochemical screening to identify secondary metabolites. Four gel formulations were prepared using carbopol as the base: F0 (without extract), F1 (0.1% extract), F2 (3% extract), and F3 (5% extract). The formulations were evaluated for organoleptic properties, homogeneity, pH, spreadability, viscosity, physical stability through cycling tests, and antibacterial activity against Staphylococcus epidermidis. Phytochemical screening of the extract revealed the presence of alkaloids, tannins, flavonoids, saponins, triterpenoids, and glycosides, indicating the extract's potential antibacterial properties. The butterfly pea extract was successfully formulated into a gel that met acceptable quality standards using carbopol 940 as the gelling agent. Antibacterial tests demonstrated that the formulated gel exhibited inhibitory activity against Staphylococcus epidermidis.
Antibactery Activity of Etyl Asetate Fraction of Etanol Extract of Pala Leaf (Myristica Fragrans) and Lidah Buaya Leaf (Aloe Vera) Against Streptococcus Mutans Awanis, Safirah; Mambang, D Elysa Putri; Yuniarti, Rafita; Nasution, Muhammad Amin
Indonesian Journal of Science and Pharmacy Vol. 2 No. 3 (2025): Indonesian Journal of Science and Pharmacy
Publisher : Pustaka Media Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63763/ijsp.v2i3.97

Abstract

Caries is a progressive chronic hard tissue disease of the teeth caused by the action of microorganisms. The bacteria that causes this disease is Streptococcus mutans. Nutmeg leaves (Myristica fragrans) and aloe vera leaves (Aloe vera) can potentially overcome diseases and health problems. This study was to determine the antibacterial activity of n-hexane fraction, ethyl acetate, ethanol extract of  nutmeg leaf and aloe vera leaf in Streptococcus mutans and to determine the effect of n-hexane fraction, ethyl acetate, ethanol extract of the antibacterial of nutmeg leaf and aloe vera leaf. The method uses was an experimental method with phytochemical screening, then manufacture of ethanol extracts of nutmeg leaves and aloe vera leaves, then fractionated with ethyl acetate solvent, then testes for antibacterial activity against Streptococcus mutans by the agar diffusion method. The results of the antibacterial activity test of the ethyl acetate fraction of nutmeg leaves showed the diamete of the inhibition zones at concentrations of 50%, 40%, 30%, 20%, and 10% were obtaines with inhibition zones of 16.2 mm, 15.46 mm, 15.2 mm, 12.26 mm and 11.76 mm. The test results on aloe vera leaf ethanol extract at concentrations of 50%, 40%, 30%, 20%, and 10 % were obtained with inhibition zones of 16.53 mm, 15.13 mm, 14.3 mm, 11,5 mm, and 11.3 mm. From the results of the study, it can be concluded that simplisia, ethanol extract, ethyl acetate fraction, n-hexane nutmeg leaf and aloe vera leaf contain secondary metabolites that act as antibacterial. The antibacterial activty of ethyl acetate fraction of nutmeg leaves was highest at a concentratioan of 50% with an inhibition zone of 16.2 mm and the antibacterial activity of aloe vera leaf ethanol extract was highest at a concentration of 50% with an inhibition zone of 16.53 mm in Streptococcus mutans which can be categorized as having strong inhibitory power.
Determination of Total Flavonoid Content of Ethanol Extract and Ethyl Acetate Fraction of Avocado Seed (Persea americana Mill) by Visible Spectrofotometry Methods Natasya, Siti Adinda; Nasution, M. Pandapotan; Nasution, Haris Munandar; Yuniarti, Rafita
Indonesian Journal of Science and Pharmacy Vol. 2 No. 3 (2025): Indonesian Journal of Science and Pharmacy
Publisher : Pustaka Media Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63763/ijsp.v2i3.98

Abstract

Avocado (Persea americana Mill.) has long been used in traditional medicine, and its seeds are known to contain flavonoid compounds, which are natural phenolics with antioxidant properties and potential therapeutic benefits. This study aims to identify the chemical compounds present in the ethanol extract and ethyl acetate fraction of avocado seeds, and to determine their total flavonoid content using UV-Visible spectrophotometry. This experimental study included sample collection, simplicia characterization, extraction, fractionation, phytochemical screening, and flavonoid quantification. Phytochemical screening showed that the ethanol extract contains alkaloids, flavonoids, tannins, saponins, triterpenoids, and glycosides, while the ethyl acetate fraction contains alkaloids, flavonoids, tannins, steroids, and glycosides. Flavonoid content was determined using quercetin as a standard. The results showed that the total flavonoid content in the ethanol extract was 8.655 ± 0.144 mgQE/g, while the ethyl acetate fraction contained 32.828 ± 0.105 mgQE/g. It can be concluded that the ethyl acetate fraction contains a significantly higher level of flavonoids compared to the ethanol extract.
KONSENTRASI HAMBAT MINIMUM (KHM) DAN KONSENTRASI BUNUH MINIMUM (KBM) EKSTRAK DAUN KEDONDONG HUTAN (Spondias pinnata (L.f) Kurz.) TERHADAP BAKTERI Escherichia coli Pertiwi, Rahma; Haris Munandar Nasution; Rafita Yuniarti; Zulmai Rani
JURNAL FARMASI, SAINS, dan KESEHATAN Vol. 5 No. 1 (2025): FARMASAINKES: JURNAL FARMASI, SAINS dan KESEHATAN
Publisher : Fakultas Farmasi Universitas Muslim Nusantara Al Washliyah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32696/farmasainkes.v5i1.5147

Abstract

Daun kedondong hutan diketahui memiliki kandungan metabolit sekunder salah satunya yaitu flavonoid. Senyawa flavonoid memiliki aktivitas sebagai antibakteri dengan mekanisme menghambat sintesis asam nukleat dan fungsi membran sel. Escherichia coli merupakan bakteri yang sering menyebabkan penyakit infeksi pada saluran pencernaan. Penelitian ini bertujuan menguji kadar hambat minimum dan kadar bunuh minimum ekstrak etanol daun kedondong hutan terhadap bakteri Escherichia coli. Kadar hambat minimum ditentukan menggunakan metode dilusi dilanjutkan dengan uji spektrofotometri UV-Vis dan kadar bunuh minimun ditentukan menggunakan metode dilusi yang disebar merata diatas media MHA dengan konsentrasi ekstrak bervariasi. Hasil penelitian menunjukkan pada konsentrasi 5% dan 7,5% merupakan kadar minimum ekstrak etanol daun kedondong hutan dalam menghambat aktivitas pertumbuhan bakteri Escherichia coli. Penelitian ini menunjukkan ekstrak etanol daun kedondong hutan dapat digunakan sebagai alternatif antibakteri dan dapat dikembangkan lebih lanjut pada bidang kesehatan.
ISOLASI HEMISELULOSA DARI TANDAN KOSONG KELAPA SAWIT (Elaeis guineensis Jacq.) SEBAGAI EMULGATOR DALAM SEDIAAN EMULSI MINYAK IKAN Syafirna, Nada; Dikki Miswanda; Lubis, Minda Sari; Yuniarti, Rafita
JURNAL FARMASI, SAINS, dan KESEHATAN Vol. 5 No. 1 (2025): FARMASAINKES: JURNAL FARMASI, SAINS dan KESEHATAN
Publisher : Fakultas Farmasi Universitas Muslim Nusantara Al Washliyah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32696/farmasainkes.v5i1.5192

Abstract

Tandan kosong kelapa sawit (TKKS) merupakan limbah padat yang dihasilkan dari industri pengolahan kelapa sawit. Diketahui TKKS mengandung hemiselulosa yang dapat dimanfaatkan dalam bidang farmasi. Tujuan penelitian ini untuk mengisolasi hemiselulosa dari TKKS dan memformulasikan sebagai emulgator pada sediaan emulsi minyak ikan. Penelitian ini dilakukan dengan metode eksperimental dengan tahapan meliputi isolasi hemiselulosa TKKS dengan menggunakan metode ekstraksi alkali dengan NaOH 0,05N, 0,1N, 0,2N, 0,3N, 0,5N, kemudian karakterisasi hemiselulosa meliputi uji organoleptis, uji kelarutan, uji FTIR (Fourier Transform Infra Red) dan uji SEM (Scanning Elektron Microscope). Hemiselulosa yang telah diperoleh digunakan sebagai emulgator dalam sediaan emulsi, kemudian dilakukan uji evaluasi sediaan seperti uji organoleptis, uji homogenitas, uji pH, uji tipe emulsi, uji redispersi, dan uji viskositas. Hasil dari isolasi hemiselulosa TKKS mendapatkan hasil terbaik pada konsentrasi NaOH 0,5N menghasilkan randemen 12,84%. Hasil formula sediaan emulsi kombinasi hemiselulosa yang terbaik adalah F5 dengan perbandingan gom arab : hemiselulosa (2:1). Sediaan emulsi homogen, hasil uji pH formula F1 dan F5 memenuhi syarat pH emulsi oral pada rentang 5-7,5. Hasil uji tipe emulsi yaitu A/M, hasil uji viskositas F1, F3, F4, F5 sudah memenuhi syarat sesuai SNI yaitu 2000-50.000 cPs. Hemiselulosa TKKS dapat menjadi emulgator pada emulsi minyak ikan pada formula kombinasi.
Liquid Soap with Pineapple Hump Extract and Nanoformulation Against Staphylococcus aureus Lubis, Minda Sari; Fauzi, Ziza Putri Aisyia; Dewi, Sri Harti; Rani, Zulmai; Yuniarti, Rafita
Sciences of Pharmacy Volume 4 Issue 3
Publisher : ETFLIN Publishing House

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58920/sciphar0403348

Abstract

Staphylococcus aureus is a Gram-positive bacterium that frequently causes skin infections and can become resistant to various antibiotics. Pineapple (Ananas comosus) waste, particularly the hump, contains active compounds such as bromelain, saponins, and flavonoids with known antibacterial properties. This study aimed to determine the antibacterial potential of liquid soap formulated with extract and nanoextract of pineapple hump against S. aureus. The pineapple hump was extracted using maceration with 96% ethanol, followed by nanoparticle formulation using a high-speed homogenization method. Liquid soap was made with three formulas, one formula contains a concentration of 12.5% extract and two formulas contain 1.25% nanoextract of pineapple hump. Antibacterial activity was evaluated using the disc diffusion method, and the diameter of the inhibition zones was measured. Results showed that all tested concentrations produced antibacterial activity, with the 12.5% concentration (Formula 1) exhibiting the highest inhibition zone (23.2 mm), followed by 1.25% nanoextract nano liquid soap (18.41 mm) (Formula 3) and 1.25% nanoextract liquid soap (14.53 mm) (Formula 2). The positive control (Dettol Handwash) produced a larger inhibition zone (20.08 mm). Data analysis using one-way ANOVA revealed significant differences for each formula, with a p-value of less than 0.05. These findings indicate that nanoextract of pineapple hump formulated in liquid soap has potential as a natural antibacterial agent against S. aureus.
Co-Authors A'dilah, Nur Adela Octi Dwiyani Adelia Ramadani Adjelie, Tiara Afifah, Puan Ibni Afriati, Nelsi Afrida Yeti Aliza, Ulfa Andjelie, Tiara Anggraini, Dea Puspa Ani Sutiani Anny Sartika Daulay Ariandi Arianti, Sellin Arina Asmarani, Asmarani Aula, Rizki Pebri Aulia Fitri, Aulia Aulia, Meisyah Awanis, Safirah Bilal, Mohd. Chairina Milda Cindy Marlina Tambunan Cut Erika Maulydya D. Elysa Putri Mambang D. Elysa Putri Mambang Dama Yanti, Fradilla Daniel Happy Putra Debi Meilani Dewa, Fasca Dewi Firmayani Dewi, Sri Harti Dikki Miswanda Dina Veranika Dinda Sari Utami Fahmi Fahmi Farida Yani Fauziah Zain Fithri Pulungan, Ainil Fitri Febriani Fitri, Raissa FRANSISKA, EVA Gabena Indrayani Dalimunthe Haris Munandar Nasution Hendraputra, Hardy Handoko Heru Adha Prayogo Humaira, Yara Hutagalung, Riris Br Hutagalung, Victor H Ika Fitriani Indah Nasution Indri Juliarni Inna Myesha Jurida, Wike Juwita, Sukma Karismawati Karlina Butar Butar Khairina Khairunnisa , Ardina Kiki Rawitri Linda, Rosa Lisda Mayanti Lubis, Minda Sari Lubis, Nurazizah Lubis, Zulkifli Ismail Luthfianida, Arifna M Pandapotan Nasution M. Naufal Rifqi M. Pandapotan Nasution Maharani, Suci Mambang, D Elysa Putri Maulana Putri, Yasinta Mayani, Nurhikma Maysarah, Aulya Metami Sihombing, Juliana Meylisa Pratami Br Sinaga Milwani Harahap Minda Sar Lubis Miranza, Nona Misna Rosalinda Hutabarat Muhammad Amin Nasution Muhammad Ari Mukhtizar Muhammad Pandapotan Nasution Muhammad Tegar Tri Rizky Muslim, Nur’Aini Jumah Nasri Nasri, Nasri Nasution, M. Pandapotan Natasya, Siti Adinda Nia Novranda Pertiwi Novi Yuliandari Novia Sari Novita Sari, Helsa Nur Asia, Nur Nur, Hijrotun Nuratika, Eli Nurfadilla, Kiki Nurliansyah, Nurliansyah Nursyafira, Arini Oktavia, Zela Pasaribu, Mesi Wilia Afrima Pasaribu, Mesi Willia Afrima Peri , Peri Peri, Peri Pertiwi, Rahma Purba, Juvantri Fablo Puspita, Dinda Dila Puteri, Cut Intan Annisa Putri Theresia Harianja Putri, Nadya Iwani Rahma Maulidia Fitri Rambe, Sitiapsah Reza Irma Riani, Nur Aslin Ricky Andi Syahputra Ridwan Taher Lubis Ridwanto Ridwanto Ridwanto Rifqi, M. Ainur Risma Fauziah Pasaribu Ritonga , Septi Ani Ritonga, Oktavia Rukmana, Siti Sagita Marina Simatupang Samosir, Anzly Hasian Saragih, Aqilah Mutmainnah Sari Lubis, Winda Sari, Syilvi Rinda Sartika Daulay, Anny Shinta Mida Ariani Harahap Siagian , Anggi Yani Sihotang, Laura Indah Restu silalahi, zahra salsabila Sindy Putri Utami Siregar , Runisya Maugin Utami Siregar, Hikmatussabaria Siregar, Rina Paramitha Siti Anisa Sri Harti Sri Mulia Ningsih Siregar Sri Murni Sri Natalia Saragih Sri Ria Ranti Sri Wahyuni Sulwiyatul K Sani Sunita, Della Syafa Nadira Ashiilah Syafirna, Nada Syahputra, Ricky Andi Syarifah Aulia Tasya Ardana Tia Nazilla Tri Damaiyanti Ummi Khairani Rambe Ummu Safura Sirait Vera Estefania Kaban Wahdaniati , Tri Wijiyaningsih, Dinda Wilda Septia Winda Aulia Yayuk Putri Rahayu Yayuk Putri Rahayu Yuni Sartika Zahara, Fitria Siti Zannah , Muhamad Zhafirah, Rana Ziza Putri Aisyia Fauzi Zulhij, Fizrya Zulmai Rani