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KHOIRUL ANWAR
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Journal : Natural Sciences Engineering and Technology Journal

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Quality Control of Sunscreen Products: A Validated HPLC Method for the Analysis of Avobenzone and Oxybenzone Novy Inti Fauziah; Aqnes Budiarti; Khoirul Anwar
Natural Sciences Engineering and Technology Journal Vol. 5 No. 1 (2025): Natural Sciences Engineering and Technology Journal
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/nasetjournal.v5i1.63

Abstract

Avobenzone and oxybenzone are commonly used ultraviolet (UV) filters in sunscreen products, offering broad-spectrum protection against harmful solar radiation. Accurate determination of these compounds in sunscreen formulations is crucial for quality control and ensuring consumer safety. This study aimed to develop and validate a high-performance liquid chromatography (HPLC) method for the simultaneous determination of avobenzone and oxybenzone in commercial sunscreens. The HPLC method utilized a C₁₈ column with a mobile phase of methanol:aquabidest (93:7, v/v) at a flow rate of 1.0 mL/min and a detection wavelength of 320 nm. The method was validated for linearity, sensitivity, selectivity, precision, and accuracy. The validated method was then applied to quantify avobenzone and oxybenzone in three different brands of commercial sunscreen products. The developed HPLC method demonstrated excellent linearity for both avobenzone (r = 0.9998) and oxybenzone (r = 0.9995). The method was also highly sensitive with low limits of detection (LOD) and quantitation (LOQ) for avobenzone (0.13 μg/mL and 0.43 μg/mL, respectively) and oxybenzone (0.35 μg/mL and 1.15 μg/mL, respectively). The method exhibited good selectivity and precision (%RSD ≤ 2%). Accuracy, as determined by recovery experiments, was within the acceptable range (100.01%-100.77% for avobenzone and 99.66%-100.81% for oxybenzone). The levels of avobenzone and oxybenzone in the analyzed sunscreen brands (A, B, and C) were found to be within the regulatory limits. In conclusion, the validated HPLC method provides a reliable and efficient means for the simultaneous quantification of avobenzone and oxybenzone in sunscreen products, contributing to the quality control and safety of these widely used formulations.
Co-Authors Abdillah Afabih Abdillah Nurul Bahri Abdullah . Achmad Agus Priyono Adi Iskandar Aditya Ramadhan Ahmad Hariyadi, Ahmad Ahmad Muthohar Ahmadi M Akhsan Akhsan Alif Rahman Habibi Anggia Azizah Ani Rosita Aqnes Budiarti Asa Alvi Zahroh Asrorul Mais Avivah Nur Avnida Maulana Awali Nur Misbahudin Ayu Shabrina Azizul Berlyansah Badjuri Badjuri Bidzlamira Dita Centrisia Budi Prasetya Candra Hadi Asmara Dadi H Maskar Dharmawan Dharmawan Dharu Arseno Dian Ahkam Sani Dimas Ersantyo Dio Resya Baaqii Dwi Prebrianti Dwi Sasongko Mardiono Endang Tyasmaning Eni Winarsih Faiq Fauzilla Faradilla Ulya Mudyana Fitria Mauilida Gatut Setiadi Hadi Sunaryo Haryadi Haryadi Heni Pujiastuti Heni Yuli Handayani Heroweti, Junvidya Hery Sahputra Hidayatullah, Fajar Hilman Auzan Mulyono Hufron Ibrahim Arifin Inna Hamida Zusfindhana Isti Rahayu Jamhuri Jihan Fitriyah Junaidin Sirza, La Ode Muhammad Latifatul Karimah Lazim N Lazim N Lisatul Aulia Lusmino Basia M. Aji Sadewo M. Darul Fathoni M. Lathif Bashar M. Naqsyabandi S Mahdum Zahid MARIA ULFAH Meyla Nur Vita Sari Moh Alif Rizkiyanto Moh Ata Khoirlana Moh. Mofid Mohamad Khoiron Mohammad Zoqi Sarwani Mona Fathia Muawanah, Arofatul Muhamad Subhi Apriantoro Muhamad Zainudin Abdul Medjid Muhammad Fajar Purnama Muhammad Ikhsan Muhammad Mahrus Ali Muhammad Ridha Irsyadillah Muhammad Rifqy Asrul Muhammad Salman Alfarisi Muhammad Udin Nachwan Mufti Adriansyah Neneng Munifah S Neneng Munifah Sholilah Nihayatut Tasliyah Nindy Sabrina Nirwanto Maruf Nisa Noor Amalia Nola Gabriella Hutapea Novy Inti Fauziah Rahma Amalia Aslam Rahmad Nur Setiawan Ratna Kumala Setyaningum Rifki Khoirudin Rindra Risdiantoro Salwiyah . Septyaningrum Septyaningrum Siti Dahlia Sri Irawati Sri Mulyani Sri Suwarni Sulthon Faryabi Nurbadri suparman suparman Syakilah Al Mutawakkil Arifin Titiek Suheta Urva Fresiva Vivien Carin Wahyu Ramadhani Rambe Wira Fazri Rosyidin Witri Winanda Wiwik Sri Handayani Wulida Ainur Rofiq Yeshika Maharani Yusnita Rahayu Zaenah Amala Ummi Robihah Zizi Nurhikmah