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Pengaruh Irradiasi Sinar Gammapada Pertumbuhan Kalusdan Tunas Tanaman Gandum (Triticum aestivum L.) Laela Sari, Agus Purwito, Didy Sopandie, Ragapadmi Purnamaningsih, dan Enny Sudarmanowa
Jurnal Ilmu Pertanian Vol 18, No 1 (2015): April
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (398.021 KB) | DOI: 10.22146/ipas.6176

Abstract

Tujuan utama pemuliaan tanaman adalah memperbaiki varietas yang sudah ada guna mendapatkan varietas yang lebih baik atau unggul. Tujuan dari penelitian ini adalah untuk mengetahui kisaran Lethal Dosis 20 (LD20) dan 50 (LD50) pada kalus gandum varietas Dewata yang diradiasi sinar gamma. Keberhasilan perlakuan irradiasi sinar gamma sangat ditentukan oleh sensitivitas genotipe tanaman. Penelitian ini dilakukan mulai bulan Juli-Desember 2011 di Laboratorium kultur BB-Biogen dan PATIR BATAN. Percobaan disusun secara faktorial dalam lingkungan Rancangan Acak Lengkap 1 x 4, dengan 3 ulangan. Faktor pertama adalah genotipe gandum Dewata. Faktor kedua adalah dosis irradiasi sinar gamma yaitu 0; 7,5; 15; 22,5; dan 30 Gy. Peubah yang diamati pertumbuhan kalus dan persentase tumbuh tunas. Data dianalisis menggunakan Program SAS 9.1. LD20 dan   LD50  ditentukan menggunakan Program Best Curve-fit Analysis. Hasil penelitian menunjukkan bahwa dosis irradiasi berpengaruh nyata terhadap penghambatan pertumbuhan kalus gandum. Persentase hiduptunasmenurun sejalan dengankenaikan dosis irradiasi.Kelangsungan hiduptunasmenurun drastis bahkan tidak tumbuh sama sekali dan berwarna albino pada dosis 30 Gy. Persentase hidup tunas tertinggi pada dosis 15 Gy. Persentase tumbuh kalus tertinggi terdapat pada dosis 7,5 Gy. Semakin tinggi dosis iradiasi yang digunakan, maka persentase tunas yang tumbuh semakin sedikit atau daya regenerasi semakin rendah. Peningkatan keragaman genetik kalus gandum terdapat pada perlakuan irradiasi antara LD 20 dan LD 50 yaitu  dosis  iradiasi sinar gamma 15 – 22.5 Gy. Model dosis lethal terbaik untuk pertumbuhan kalus berbentuk kuadratik dengan persamaan Y =   86,28 – 6,47 x + 0,13 x2,   LD20 = 0,99 Gy dan LD50 = 6,41 Gy. Sedangkan untuk persentase kalus yang bertunas dan mampu bergenerasi mempunyai persamaan berdasarkan persentase tumbuh kalus Y = 100,28 – 0,87x - 0,05 x2, LD20 = 13,14 Gy dan LD50 = 24,00 Gy.Kata kunci: Gandum (Triticum aestivum), lethal dosis, irradiasi sinar gamma.
CHARACTERIZATION OF SOME MORPHOLOGY, ANATOMY AND PHYSIOLOGY OF THE WHEAT MUTANT (Triticum aestivum L.) DEWATA AND SELAYAR IN TROPICAL LOWLAND. Laela Sari; Agus Purwito; Didy Sopandie
Widyariset Vol 1, No 1 (2015): Widyariset
Publisher : Pusbindiklat - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (685.363 KB) | DOI: 10.14203/widyariset.1.1.2015.%p

Abstract

Wheat mutant characterization is a part of the breeding programs to investigate the diversity that can influence in production increased. The  aim of this research was to obtain data characterization morphology, anatomy and physiology that could be used as selection criteria and obtain an adaptive wheat mutant in the tropical lowland area. The research was conducted in Seameo-Biotrop Experimental Garden, located on ±250 m above the sea level, from April until November 2013. There were 18 Dewata wheat mutants and two Selayar wheat mutants, which is an M3 derivative resulted from EMS treatment, were used. LC50 Dewata got the 0.3% EMS treatment for 30 minutes, while LC50 Selayar got the 0.1% EMS treatment for 60 minutes. The data analysis used Augmented Designs Method. The results showed that the mutant Dewata that was significantly affected the morphology was indicated by four characters i.e. cooking time (two mutants), grain weight per genotype (seven mutants), leaf area (five mutants), and green leaves (one mutant). There are two characters in mutant Selayar that bring significant influence on morphological factors, those are seed weight per genotype (one mutant) and leaf area (one mutant), while the cooking time and green leaves have no real effect. Anatomical character appearance on leaf thickness and the size of stomata showed different levels of tolerance between Dewata plant mutant (DW-0,3.30-2-13-3), Selayar mutant (SL-0,1.60-2-14-2), and its both controls. As for the physiological character, there were significant differences in the amount of proline and glucose levels. Proline level in Dewata control was 79.29 µg/gBB, while in DW mutant -0,3.30-2-13-3 was 332.37 µg/gBB. Proline levels in Selayar control was 201.53 µg/gBB, while in mutant SL mutant-0,1.60-2-14-2 was 335.79µg/gBB. Likewise, glucose level in Dewata control was 14.32mg/gBB, while in DW mutant 0,3.30-1-15-1 was 29.06 mg/gBB, Selayar controls (5.87 mg /GBB) with SL mutant-0,1.60-2-14-2 (17.68 mg /gBB).
Optimasi Regenerasi Padi Indica melalui Jalur Organogenesis Rossa Yunita; Nurul Khumaida; Didy Sopandie; Ika Mariska
Jurnal AgroBiogen Vol 13, No 1 (2017): Juni
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v13n1.2017.p35-42

Abstract

Regenerasi tanaman merupakan tahapan penting yang perlu dikuasai sebelum diaplikasikan untuk perakitan varietas unggul secara kultur in vitro. Penelitian bertujuan mengoptimasi teknik regenerasi in vitro beberapa varietas padi indica melalui jalur organogenesis. Percobaan menggunakan Rancangan Acak Lengkap yang disusun secara faktorial. Materi yang dicobakan, yaitu Ciherang, Inpari 13, Inpara 3, Pokkali, dan IR29. Perlakuan yang diberikan untuk induksi kalus organogenik adalah 2,4-D (0, 1, 3, 5, 7 mg/l) + kasein hidrolisat 3 g/l, untuk regenerasi kalus membentuk tunas adventif adalah BA (0, 1, 5 mg/l) + zeatin (0, 0,1, 0,3 mg/l) + prolin 100 mg/l, untuk multiplikasi tunas adalah MS + thidiazuron/TDZ (0, 0,1, 0,3 mg/l) dan untuk perakar-an adalah IBA (0, 1, 2, 3 mg/l). Hasil penelitian menunjukkan media terbaik untuk induksi kalus untuk padi varietas Ciherang, Inpari 13, dan Pokkali adalah MS + 2,4-D 3 mg/l, sedangkan untuk varietas Inpari 3 dan IR 29 adalah MS + 2,4-D 5 mg/l. Media terbaik untuk regenerasi tunas untuk varietas Ciherang adalah BA 5 mg/l + zeatin 0,1 mg/l + prolin 100 mg/l, untuk Inpari 13 adalah MS + BA 3 mg/l + zeatin 0,3 mg/l + prolin 100 mg/l, untuk Inpara 3 adalah BA 5 mg/l + zeatin 0,3 mg/l + prolin 100 mg/l dan untuk Pokkali dan IR29 adalah MS + BA 3 mg/l + zeatin 0,1 mg/l. Media terbaik untuk multiplikasi tunas adalah MS + TDZ 0,3 mg/l dan untuk induksi perakaran adalah MS + IBA 2 mg/l. Setiap tahapan kegiatan pembentukan planlet membutuhkan formulasi media yang berbeda.
Pengembangan Tanaman Sela di Bawah Tegakan Tanaman Tahunan Didy Sopandie; Trikoesoemaningtyas Trikoesoemaningtyas
Iptek Tanaman Pangan Vol 6, No 2 (2011): Desember 2011
Publisher : Puslitbang Tanaman Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Development of Crops Variety Tolerant to Low-light Intensity for Intercropping. The effort to increased food crops production is limited by the lack of arable land. Therefore, there is a need to utilize marginal areas such as land under estate crops which is partly shaded and hence have low light intensity as well as high acidity and drought condition. Production increase is marginal areas, such as lands under canopies of estate crops, could be achieved by improvement in (1) crop adaptation to abiotic stresses and resistance to biotic stress, (2) yield potential, and (3) cultural techniques which are based on knowledge in plant physiology and ecophysiology. A collaborative research on plant physiology, molecular biology and plant breeding is affected to be able to create approviate technologies to overcome those problems. Food crops which are potential to be produced under shaded condition as intercrops are upland rice, soybean, taro, elephant foot yams, and legume crops. Plant adaptation to low-light intensity is achieved through efficiency in light interception (avoidance) and utilization (tolerance). Through integrated research of interdisciplinary approach several promising lines of soybean having high productivity under 50% shading were selected.
Evaluasi Toleransi Plasma Nutfah Padi terhadap P Rendah di Tanah Sawah Abd. Aziz Syarif; Didy Sopandie; M. A. Chozin; K. Idris; Suwarno Suwarno
Buletin Plasma Nutfah Vol 16, No 1 (2010): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v16n1.2010.p8-16

Abstract

A field experiment was conducted to assessgenotypic variability of tolerance and to identify tolerantgenotypes to low phosphorus (P) among rice germplasm onlowland (paddy) rice field with low available and potential Pusing strip plot design with three replications. P fertilization(with and without added P) was assigned as horizontal factorand 120 rice plant genotypes as vertical factor. Data on plantheight, tiller number, and shoot dryweight were collected. LowP tolerance was judged by relative value i.e. observation valueat the plot without P treatment devided by the value at the plotwith P treatment. The judgement was determined based onIRRI standard. Shoot P uptake of five tolerant and fivesensitive genotypes on no added P treatment was alsoobserved. The results showed that the germplasm exhibitedphenotypic and genotypic variability of low P tolerance. Thehighest genotypic variability of tolerance was shown by thevalue based on relative tillering number (28.7%), followed bythat of relative shoot dryweight (22,6%) and relative plantheight (7,6%). The highest broadsense heritability of tolerancewas shown by the value based on relative tiller number(24.1%), followed by that of relative plant height (16.6%) andrelative shoot dry weight (15.0%) Fourteen genotypes wereidentified as highly tolerant based on relative tiller number and28 genotypes based on relative shoot dryweight. The toleranceof genotype was attained by higher P uptake and higherinternal use efficiency.
The Role of Arbuscular Mycorrhizal Fungus (Gigaspora margarita) on Mercury and Nutrients Accumulation by Enterolobium cyclocarpum Seedlings HANNA ARTUTI EKAMAWANTI; YADI SETIADI; DIDY SOPANDIE; DWI ANDREAS SANTOSA
Microbiology Indonesia Vol. 7 No. 4 (2013): November 2013
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (154.129 KB) | DOI: 10.5454/mi.7.4.5

Abstract

A river-sand culture experiment was conducted to investigate whether arbuscular mycorrhizal (AM) colonization influenced mercury (Hg) and nutrients accumulation, and whether AM fungus (AMF) Gigaspora margarita enhance host plant sengon buto (Enterolobium cyclocarpum) tolerance to Hg. Hg was applied as HgCl2 at different levels (375 and 750 µM) and added to the full strength of Hoagland's solution, then applied to seedlings in river-sands as growth media according to treatments.  The non-mycorrhizal and mycorhizal E. cyclocarpum roots took up Hg, but its translocation to the leaves was inhibited. AM inoculation decreased significantly Hg content of roots seedlings by 70.5% from non-AM inoculation seedlings. Mycorrhizae enhanced significantly Ca and Mg uptake in shoot by 1.29- and 1.27-fold higher than non-mycorrhizal seedlings, but not enhanced significantly P uptake. Based on the roots dry weight, the tolerance index of non-mycorrhizal or mycorrhizal seedlings treated with 750 µM Hg supply was > 50%. It indicated that the seedlings can tolerate up to 750 µM Hg added. Considering the possible differences in AMF response to Hg in polluted soil from the field, it is not yet clear if Gi. margarita could be applied for phytoremediation of Hg in contaminated sites. Therefore, more work needs to be done using AMF isolates to reveal the possible application in the management of Hg contaminated soils.
PEWARISAN GEN PENANDA HPT(HYGROMYCINE PHOSPHOTRANSFERASE) BERDASARKAN ANALISIS PCR DAN EKSPRESINYA PADA POPULASI PADI TRANSFORMAN MENGOVEREKSPRESIKAN GEN HD ZIP OSHOX-6 Enung Sri Mulyaningsih; Hajrial Aswidinnoor; Didy Sopandie; Pieter BF OuwerkerV; Inez Hortense Slamet Loedin
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i1.2051

Abstract

First generation (TO) transgenic plants do not always segregate their transgenes in a Mendellian segregation pattern. Moreover,instability of heterologous gene expression was often observed in transgenic plants. This phenomenon is often called gene silencing. Gene silencing could happen on different level of gene expression, notably at transcriptional or post-transcriptional level.The purpose of this research was to identify the transgene segregation pattern of a marker gene (hpt) as well as the introduced- regulator gene (OsLEA-oshox6) in second generation (Tl) transgenic rice plants.Gene segregation (hpt) analysis was carried out using PCR method. Gene expression analysis was done by hygromycin antibiotic resistant test of leaf samples.Analysis was carried out on 17 lines of Tl transgenic rice plants from Batutegi and Kasalath cultivars.Mendellian segregation pattern of 3:1 was revealed for all lines based on PCR analysis. Gene expression analysis showed almost all lines was segregated in a Mendellian fashion except for Tl-BT III 2C line. Less transgenic plants that expressed the hpt gene were suggested due to gene silencing effects.It was suggested to happen at transcriptional level.
POTENSI BAKTERI ENDOFIT DALAM UPAYA MENINGKATKAN PERTUMBUHAN, PRODUKSI, DAN KANDUNGAN ANDROGRAFOLID PADA TANAMAN SAMBILOTO GUSMAINI GUSMAINI; SANDRA ARIFIN AZIZ; ABDUL MUNIF; DIDY SOPANDIE; NURLIANI BERMAWIE
Jurnal Penelitian Tanaman Industri Vol 19, No 4 (2013): Desember 2013
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v19n4.2013.167-177

Abstract

ABSTRAKBakteri endofit hidup di dalam jaringan tanaman yang sehat danberperan antara lain di dalam memacu pertumbuhan tanaman denganmenghasilkan senyawa-senyawa zat pengatur tumbuh, seperti IAA, GA 3 ,dan Sitokinin. Tujuan penelitian ini untuk mengevaluasi potensi bakteriendofit dalam  meningkatkan pertumbuhan, produksi, dan kadarandrografolid pada tanaman sambiloto. Penelitian dilakukan di rumah kacaBalittro Cimanggu Bogor pada Oktober 2011–Mei 2012. Perlakuandisusun mengikuti Rancangan Acak Kelompok, enam perlakuan danempat ulangan. Perlakuan terdiri dari (1) kontrol, dan perlakuan bakteriendofit yaitu (2) 20BB, (3) 5MD, (4) 20BD, (5) 20CD (perlakuan 2-5masing-masing terdiri dari 4 jenis isolat), dan (6) 90AA (isolat tunggal).Suspensi bakteri endofit (50 ml/tanaman) diberikan 4 kali yaitu padaminggu ke 3, 5, 7, dan 9 setelah tanam dengan konsentrasi 10 10 spk/ml.Hasil penelitian menunjukkan bahwa bakteri endofit berpengaruh postifdan nyata dalam meningkatkan pertumbuhan, produksi herba segar dankering serta andrografolid pada tanaman sambiloto lebih baikdibandingkan kontrol. Peningkatan pertumbuhan tertinggi ditunjukkanpada tinggi tanaman dan jumlah cabang primer yaitu masing-masing24,7% (20 CD) dan 42,2% (20 BB). Produksi herba kering meningkat 25-82,81%, sejalan dengan meningkatnya serapan hara N (64,7-158,8%), P(50-100%), dan K (65-155%). Peningkatan produksi herba kering danandrografolid terbaik diperoleh dari penggunaan 20 CD (82,81 dan142,11%), 20 BB (88,75 dan 131,58%), dan 20 BD (65,63 dan 131,58%).Implikasi dari hasil penelitian ini bahwa bakteri endofit berpotensi untukdikembangkan pada budidaya tanaman sambiloto.Kata kunci: Andrographis paniculata, bakteri endofit, andrografolid,pertumbuhan, produksiABSTRACTEndophytic bacteria live within healthy plant tissue and playimportant roles, such as producing compounds of plant growth regulatorssubstances such as IAA, GA 3 , and Cytokinin. The aims of this research isto evaluate the potential of endophytic bacteria to promote the growth,andrographolide content, and dry matter yield of king of bitter. Theresearch was conducted in the greenhouse of Cimanggu Balittro in October2011-May 2012. Treatments were arranged in a randomized completeblock design with six treatments and four replications. Treatments consistof (1) control, and 5 kinds of endophytic bacteria isolates such as (2)20BB, (3) 5MD, (4) 20BD, (5) 20CD (treatments no.2-5, consisted of 4types of isolate), and (6) 90AA (single isolate). The highest presentage ofplant height and number of primary branches were obtained from thetreatment of 20CD (24.7%) and 20BB (42.2%). Increase in the dry herbyield of 25-82.81% was in agreement with increasing in uptake of N (64.7-158.8%), P (50-100%), and K (65-155%). The best treatment with whichyielding high of dry herbs and andrographolide was 20CD isolates (82.81and 142.11%), followed with 20 BB (88.75 and 131.58%), and 20 BD(65.63 and 131.58%). The study implies that endophytic bacteria havepotential for development of king of bitter cultivation.Key words: Andrographis paniculata, endophytic bacteria, androgra-pholide, growth, yield
PEMANFAATAN BAKTERI ENDOFIT DAN FOSFAT UNTUK PERTUMBUHAN DAN PRODUKSI TANAMAN SAMBILOTO / Utilizing Endophytic Bacteria and Phosphate for Growth and Yieald of Andrographis paniculata Gusmaini Gusmaini; Didy Sopandie; Sandra Arifin Aziz; Abdul Munif; Nurliani Bermawie
Jurnal Penelitian Tanaman Industri Vol 22, No 3 (2016): September, 2016
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v22n3.2016.153-159

Abstract

Andrographis paniculta produce andrographolide, which have functions as raw material for medicine. One can stimulate andrographolide yield by utilizing endophytic bacteria and phosphate. This research objectives were to obtain effect of endophytic bacteria and phosphate on growth, yield and andrographolide content, N, P, and K uptake of A. paniculta. The research was conducted at the experimental garden of ISMCRI, at Cimanggu, Bogor from June 2012-February 2013. The trial was arranged in Random Block Design, factorial, 9 treatments and 3 replications. The first factor were endophytic bacteria; 1) without endophytic bacteria, 2) 20CD, and 3) 20BB. The second factor were the levels of P; 1) without P, 2) 27 kg/ha P, and 3) 54 kg/ha P. The results showed that Endophytic bacteria application and P fertilizer significantly increased plant growth, dry matter yield, andrographolide content and yield, and N, P, K uptake, but there were no interaction between endophytic bacteria and P treatments. Giving of endophytic bacteria improved content and yield of andrographolide ranging 16,9-29,9% and 37,6-45,7% respectively. A dossage of 27 kg/ha P was recommended to produce dry matter and andrographolide yield, and andrographolide content obtained 2.56%. The best andrographolide yield should be harvested at the beginning of generative phase.Keywords: Andrographis paniculata, andrographolide content, endophytic bacteria, phosphate, yield. AbstrakTanaman sambiloto merupakan tanaman penghasil andrografolid, yang berfungsi sebagai bahan baku obat. Salah satu yang dapat memacu produksi andrographolid tersebut dengan memanfaatkan bakteri endofit dan fosfat. Penelitian bertujuan untuk mengetahui pengaruh bakteri endofit dan fosfat terhadap pertumbuhan, produksi dan kadar andrografolid serta serapan hara N, P, dan K pada tanaman sambiloto. Penelitian dilaksanakan di Kebun Percobaan Cimanggu Balai Penelitian Tanaman Rempah dan Obat (Balittro), Bogor dari bulan Juni 2012-Februari 2013. Penelitian ini menggunakan rancangan acak kelompok, faktorial, 9 perlakuan dan 3 ulangan. Faktor pertama yaitu bakteri endofit; 1) tanpa bakteri, 2) 20CD, dan 3) 20BB. Faktor kedua adalah dosis pupuk P; 1) tanpa pupuk, 2) 27 kg/ha P, dan 3) 54 kg/ha P. Hasil penelitian menunjukkan bahwa bakteri endofit dan P nyata meningkatkan pertumbuhan, produksi biomas, kadar dan produksi andrografolid, serta serapan hara N, P, dan K pada umur tanaman 14 MST, namun tidak terdapat interaksi antara kedua faktor tersebut. Pemberian bakteri endofit dapat meningkatkan kadar dan produksi andrografolid, masing-masing berkisar 16,9-29,9% dan 37,6- 45,7%. Dosis 27 kg/ha P direkomendasikan untuk menghasilkan produksi bahan kering dan produksi andrografolid, dengan kadar andrografolid yang diperoleh 2,56%. Produksi andrografolid terbaik, tanaman dipanen pada fase awal generatif.Kata kunci: Andrographis paniculata, andrografolid, bakteri endofit, pertumbuhan, fosfat, produksi.
CHARACTERIZATION OF SOME MORPHOLOGY, ANATOMY AND PHYSIOLOGY OF THE WHEAT MUTANT (Triticum aestivum L.) DEWATA AND SELAYAR IN TROPICAL LOWLAND. Laela Sari; Agus Purwito; Didy Sopandie
Widyariset Vol 1, No 1 (2015): Widyariset
Publisher : Pusbindiklat - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (800.768 KB) | DOI: 10.14203/widyariset.1.1.2015.%p

Abstract

Wheat mutant characterization is a part of the breeding programs to investigate the diversity that can influence in production increased. The  aim of this research was to obtain data characterization morphology, anatomy and physiology that could be used as selection criteria and obtain an adaptive wheat mutant in the tropical lowland area. The research was conducted in Seameo-Biotrop Experimental Garden, located on ±250 m above the sea level, from April until November 2013. There were 18 Dewata wheat mutants and two Selayar wheat mutants, which is an M3 derivative resulted from EMS treatment, were used. LC50 Dewata got the 0.3% EMS treatment for 30 minutes, while LC50 Selayar got the 0.1% EMS treatment for 60 minutes. The data analysis used Augmented Designs Method. The results showed that the mutant Dewata that was significantly affected the morphology was indicated by four characters i.e. cooking time (two mutants), grain weight per genotype (seven mutants), leaf area (five mutants), and green leaves (one mutant). There are two characters in mutant Selayar that bring significant influence on morphological factors, those are seed weight per genotype (one mutant) and leaf area (one mutant), while the cooking time and green leaves have no real effect. Anatomical character appearance on leaf thickness and the size of stomata showed different levels of tolerance between Dewata plant mutant (DW-0,3.30-2-13-3), Selayar mutant (SL-0,1.60-2-14-2), and its both controls. As for the physiological character, there were significant differences in the amount of proline and glucose levels. Proline level in Dewata control was 79.29 µg/gBB, while in DW mutant -0,3.30-2-13-3 was 332.37 µg/gBB. Proline levels in Selayar control was 201.53 µg/gBB, while in mutant SL mutant-0,1.60-2-14-2 was 335.79µg/gBB. Likewise, glucose level in Dewata control was 14.32mg/gBB, while in DW mutant 0,3.30-1-15-1 was 29.06 mg/gBB, Selayar controls (5.87 mg /GBB) with SL mutant-0,1.60-2-14-2 (17.68 mg /gBB).
Co-Authors , Hamin , Kisman , Purwono , Suharsono , Syafruddin , Tasliah . SUHARSONO . SUWARNO A. HAITAMI A.A. Ketut Agung Cahyawan W Abd. Aziz Syarif ABDUL KARIM MAKARIM Abdul Munif Abdul Qadir Adolf Pieter Lontoh, Adolf Pieter Agus Purwito AHMAD JUNAEDI AKIHO YOKOTA AKIHO YOKOTA Akiho Yokota Aksarah Pas, Aris Alce Ilona Noyaa Alifiya Herwitarahman Amris Makmur Amrullah Amrullah Anas D Susila Anas Dinurrohman Susila ANGELITA PUJI LESTARI Angelita Puji Lestari Annisa Fadila Ardie, and Sintho Wahyuning Ardie, dan Sintho Wahyuning Arvita Netti Sihaloho, Arvita Netti ASMARLAILI SAHAR Atang Sutandi Azri Kusuma Dewi Azri Kusuma Dewi Budi Mulyanto Careca Sepdihan Rahmat Hidayatullah Chairani Hanum Chairani Hanum Christoph Leuschner Deden Derajat Matra Desta Wirnas Dhika Prita Hapsari DWI ANDREAS SANTOSA Edi Santosa Edi Santosa Edi Santosa Efendi, Darda Enny Sudarmonowati Enny Sudarmonowati Enung Sri Mulyaningsih Enung Sri Mulyaningsih Eny Widajati Erma Prihastanti Fadliah Salim Faqih Udin Gusmaini Gusmaini GUSMAINI GUSMAINI GUSMAINI GUSMAINI, GUSMAINI HAJRIAL ASWIDINNOOR Hajrial Aswidinoor Halimatus Syahdia Hasibuan Hamim Hamim Hanedi Darmasetiawan HANNA ARTUTI EKAMAWANTI Hapsoh Hidayah, Agus Nur I Ketut Suada IBNUL QAYIM Ida Hanarida Soemantri Ika Mariska Ika Mariska Ika Mariska Ilona Noyaa, Alce Imam Widodo Inez Hortense Slamet Loedin Inez Hortense Slamet Loedin Inez Hortense Slamet-Loedin INEZ HORTENSE SLAMET-LOEDIN Iskandar Lubis Joko Prasetiyono Joko Prasetiyono Juang Gema Kartika K. Idris Karlin Agustina Kartika Ning Tyas Ketty Suketi KINYA AKASHI KINYA AKASHI Kisman Kisman Komaruddin Idris Komaruddin Idris La Muhuria Laela Sari Laela Sari laela Sari, laela Lukman Chakim Luluk Setyaningsih Lutfy Ditya Cahyanti, Lutfy Ditya M A Chozin M. A. Chozin Maemonah, Maemonah Mahpuzah, Marfiatun Mariska, Ika Masdiar Bustamam Masdiar Bustamam Masumi Moritsugu Matra, Deden Derajat Maya Melati Mayang Sari Meliala, Merry Gloria Moh. Yani Momongan, Jorex Daniel Muhamad Syukur Muhammad Arif Yudiarto Muhammad Jusuf Munarti Munif Ghulamahdi Muzuni, Muzuni Nani Heryani Nasution, Annisa Andrini Ni Made Armini Wiendi Nurliani Bermawie Nurliani Bermawie NURLIANI BERMAWIE Nurul Khumaida Osmat Azzam Jefferson Osmat Azzam Jefferson, Osmat Azzam OuwerkerV, Pieter BF Pieter B.F. Ouwerkerk Pieter BF OuwerkerV Putri Andini Mandasari RADITE TISTAMA RAGAPADMI PURNAMANINGSIH Ragapadmi Purnamaningsih Ratna Yuniati Rini, Erin Puspita Roedhy Poerwanto Rossa Yunita Rossa Yunita Rossa Yunita Rosyad, Astryani S. Suharsono Saepudin, Adam Sandi, Yusri Iryas Sandra Arifin Aziz Saragih, Ery Leonardo Sari, Laela Sarsidi Sastrosumarjo SATRIYAS ILYAS Satya Nugroho Sinaga, Falencia Sinaga, Parlin H. Sintho Wahyuning Ardie Siti Marwiyah Slamet Loedin, Inez Hortense Sobir Sobir SOEKISMAN TJITROSEMITO Soeranto Human Sri Mulatsih SRI RAHAYU Sri Setyati Harjadi Sri Wilarso Budi Sudarmonowati, Enny Sudirman Yahya Sudirman Yahya Sugianta Sugianta Sugianta, Sugianta SUGIONO MOELJOPAWIRO Sugiono Moeljopawiro Suharsono . Suharsono Suharsono Suharsono Suharsono Suhartanto, Muhammad Rahmad Sumiati, dan Sungkono Sungkono Supijatno Surjono Hadi Sutjahjo Suwarno Suwarno Suwarno Suwarno Suwarto Suwarto Syamsidah Rahmawati Syamsidah Rahmawati, Syamsidah T. Kawasaki T. M. H. Oelim Tanari, Yulinda Tintin Suhartini Titin Handayani Toshio Kawasaki Toyip Toyip TRI KOESOEMANINGTYAS Tri Lestari Trikoesoemaningtyas Utami, Nita Nur UTUT WIDYASTUTI UTUT WIDYASTUTI Utut Widyastuti Utut Widyastuti Suharsono Wage Ratna Rohaeni Wahju Q. Mugnisjah Wahju Qamara Mugnisjah Yadi Setiadi Yadi Setiadi Yadi Setiadi YADI SETIADI Yonny Koesmaryono Yopie Moelyohadi Yuli Sulistyowati YULIN LESTARI Yuniati, Ratna Yunita, Rossa