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Morphophysiological Changes of Mangosteen Seedling (Garcinia mangostana L.) on Polyethylene Glycol (PEG) Application Dhika Prita Hapsari; Roedhy Poerwanto; Didy Sopandie; Edi Santosa; Deden Derajat Matra
Jurnal Hortikultura Indonesia Vol. 11 No. 1 (2020): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.11.1.1-12

Abstract

Mangosteen requires drought condition to induce flowering, however extending drought period might restrict the plant growth. Therefore, the response of mangosteen to drought stress needs to be studied. This research conducted on May to July 2017 using Randomized Block Design with one factor. The experiment comprised of 4 levels of polyethylene glycol (PEG 6000) treatment, i.e., PEG 0%, PEG 10% (-0.03 MPa), PEG 15% (-0.41 MPa) dan PEG 20% (-0.67 MPa) (m.v-1). The results showed that there is a dryness of the mangosteen leaves given PEG which occured slowly, starting from the edge of the leaves into the center of midrib. Water consumption was fluctuated until the end of experiment, which the highest was 33.33 ml per day in 0% PEG treated plant, then decreased until 10 ml per day in 20% PEG treated plant. Proline content increased from the first week to the third week after all PEG treatments. The highest proline content were found in 15% PEG (22.14 umol.g-1) and 20% PEG (23.46 umol.g-1) treated plants. Plant dry mass was low under water stress, and more severe stress resulted in more reduction of plant dry mass level. Water stress did not affect the N and Mg content significantly, but reduced P content in mangosteen seedling. Keywords: evapotranspiration, nutrient content, proline, water stress
PENGARUH NAUNGAN TERHADAP INTERSEPSI DAN EFISIENSI PEGGUNAAN RADIASI SURYA PADA TANAMAN PADI GOGOTHE EFFECT OF SHADING ON SOLAR RADIATION INTERCEPTION AND RADIATION USE EFFICIENCY OF UPLAND RICE Yopie Moelyohadi; Yonny Koesmaryono; Hanedi Darmasetiawan; Didy Sopandie
Agromet Vol. 14 No. 1 & 2 (1999): June 1999
Publisher : PERHIMPI (Indonesian Association of Agricultural Meteorology)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1569.149 KB) | DOI: 10.29244/j.agromet.14.1 & 2.59-70

Abstract

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The objective of this experiment was to screen 16 soybean genotypes tolerance to double stresses of Al toxicity and drought stress.  A pot experiment was conducted at Cikabayan Experimental Station of Bogor Agricultural University from January to Maret 2004.  Soybean plants were grown in Gajrug ultisol soil with pH 3.9 and Al saturated 26.61 me/100 g of soil.  Al toxicity and drought tolerance were measured from the root length of seedling grown in 75% Al saturation and 40% field capacity compar Chairani Hanum; Wahju Q. Mugnisjah; Sudirman Yahya; Didy Sopandie; Komaruddin Idris; Asmarlaili Sahar
Forum Pasca Sarjana Vol. 32 No. 4 (2009): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The objective of this experiment was to screen 16 soybean genotypes tolerance to double stresses of Al toxicity and drought stress.  A pot experiment was conducted at Cikabayan Experimental Station of Bogor Agricultural University from January to Maret 2004.  Soybean plants were grown in Gajrug ultisol soil with pH 3.9 and Al saturated 26.61 me/100 g of soil.  Al toxicity and drought tolerance were measured from the root length of seedling grown in 75% Al saturation and 40% field capacity compared to 25% Al saturation and 80% field capacity.  The decrease of root length much lower at 75% Al saturation and 40% field capacity. Result of the study showed that soybean of Moket, B.3799, Malabar, Wilis, Sindoro, and Slamet were tolerant while Dieng, Sinyonya, B.3780, Sicinang, B.3578, Ceneng, Epyek, MLG 3072, and Tidar were susceptible to Al toxicity and drought stress. Key words: soybean, Al toxicity, drought stress, selection
SOMACLONAL PUTATIVE MUTANTS OF RICE TOLERANT TO SALINITY Rossa Yunita; Nurul Khumaida; Didy Sopandie; Ika Mariska
Indonesian Journal of Agricultural Science Vol 19, No 2 (2018): December 2018
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v19n2.2018.p67-74

Abstract

Soil salinity could significantly reduce rice yield, therefore, varieties tolerant to salinity are urgent to be developed. Mutation induction could be used to create rice mutants tolerant to salinity. The study aimed to evaluate the tolerance of somaclonal mutants of rice to NaCl salinity in a greenhouse condition and characterize their tolerance mechanism. A total of 45 putative mutants were generated by a gamma ray mutation induction followed with in vitro selection in the growth media containing different NaCl concentrations in the greenhouse experiment. The study consisted of two-factor treatments, namely three levels of NaCl concentrations and 45 rice mutants suspected to be tolerant to salinity, arranged in a completely randomized design. Proline, cations (K, Na, Ca, and Mg) content, and stomata density were evaluated.  The results showed that eight mutants were tolerant to 150 mM NaCl, namely CH30, CH-4-2, II-13-42, II-13-7, II-13-10, II-13-13, II-13- 2, and IA-3-21. These tolerant mutants had a higher Na content compared to the check parent. The tolerant mutants had a high proline content, lower Na, and stable K, Mg and Ca cations as well as had a greater number of stomata and higher stomata length-width ratio. Some of the identified tolerant mutants demonstrated the tolerant mechanism against salinity stress. Further studies are required to evaluate these tolerant mutants in the field conditions under salinity stress.
THE TOLERANCE AND YIELD COMPONENTS OF RICE BREEDING LINES SELECTED UNDER LOW AND OPTIMUM NITROGEN CONDITIONS Angelita Puji Lestari; Suwarno Suwarno; Trikoesoemaningtyas Trikoesoemaningtyas; Didy Sopandie; Hajrial Aswidinnoor
Indonesian Journal of Agricultural Science Vol 20, No 2 (2019): December 2019
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v20n2.2019.p61-68

Abstract

One of the important issues on rice breeding is to develop new rice lines suitable for nitrogen efficiency in the suboptimum environment. The study aimed to evaluate the tolerance and yield components of rice breeding lines selected under low and high nitrogen conditions. The F6 generation from two cross-combinations of Gampai/IR77674 and Progol/Asahan, were evaluated in the dry season of 2014 under N suboptimum and N optimum conditions. A total of 172 lines plus six check varieties derived from the low and high N environment selection were evaluated under low N rate (34.5 kg N ha-1) and high N rate (138 kg N ha-1), arranged in an augmented design. Six check varieties were relocated three times in each block. Plot size was 5.5 m2 and plant spacing 20 cm x 20 cm. Data were collected for grain yield and major yield components. Results showed that the different status of nitrogen fertilizer affected the number of productive fillers, number of filled grains, 100-grain weight, and grain yield. Different cross combinations exhibited different results in the progeny performance. Ten lines have a higher yield also tolerant to low N condition, i.e. B14250F-6-9, B14250F-1-4, B14250F-9-9, B14250F-6-4, B14250F-5-2, B14262F-15-6, B14250F-2-6, B14262F-12-4, B14250F-5-1, and B14250F-11-4. Thus, to obtain the N tolerant lines, selection at a low N environment was more effective compared with the optimum selection environment.
Identifikasi Marka Polimorfik untuk Pemuliaan Padi Toleran Defisiensi Fosfor Joko Prasetiyono; Hajrial Aswidinoor; Sugiono Moeljopawiro; Didy Sopandie; Masdiar Bustamam
Jurnal AgroBiogen Vol 4, No 2 (2008): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v4n2.2008.p51-58

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Information on polymorphismsamong rice parents are very important in ricebreeding for tolerance to phosphorus defficiency. A studywas conducted at the Molecular Biology Laboratory,Indonesian Center Agricultural Biotechnology and GeneticResources (ICABIOGRAD) from October 2006 to July 2007 toidentify polymorphism markers from 6 rice genotypes. Therice genotypes, i.e., Dodokan, Situ Bagendit, Batur, Kasalath,NIL-C443, dan K36-5-1-1 were analyzed for polymorphismsusing 496 SSR markers, which cover the rice genomes.Seven of the 496 markers were used as foreground andrecombinant selection markers, and the rests (489 markers)were used as background selection markers. PCR amplificationswere separated on a 5% polyacrylamide gel andcolored by the silver staining method. Three different markersamong the seven foreground and recombinant selectionmarkers were selected from each crossing, which aretightly linked with Pup1 gene and have a distance less than 5cM. These markers are Dodokan vs Kasalath (RM277, SSR3,RM519), Dodokan vs NIL-C443 (RM277, SSR3, RM519),Dodokan vs K36-5-1-1 (RM277, SSR3, RM519), Situ Bagenditvs Kasalath (RM28102, SSR3, RM519), Situ Bagendit vs NILC443(RM28102, SSR3, RM519), Situ Bagendit vs K36-5-1-1(RM511, SSR3, RM519), Batur vs Kasalath (RM277, RM1261,RM519), Batur vs NIL-C443 (RM277, RM1261, RM519), andBatur vs K36-5-1-1 (RM28102, SSR3). Variations in backgroundselection primers were found in each chromosomeand in each parent combinations. Primers on chromosome4, 5, and 12 showed the lowest polymorphisms; moreprimers are needed for these chromosomes.
Pengaruh Iradiasi Sinar Gama terhadap Pertumbuhan dan Regenerasi Kalus Padi Varietas Ciherang dan Inpari 13 Rossa Yunita; Nurul Khumaida; Didy Sopandie; Ika Mariska
Jurnal AgroBiogen Vol 10, No 3 (2014): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v10n3.2014.p101-108

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Effect of gamma irradiation on rice callus dependson the irradiation dose used. Irradiation dose is one of thefactors that affect the genetic changes in the cells of plants.High doses can result in tissue death, meanwhile low doseswill result in abnormal changes in plant phenotype. The levelof sensitivity of a plant tissue against gamma irradiation isdifferent. This study aimed to evaluate the growth andregeneration of callus in various irradiation doses of gammaray. The plant materials used were mature embryos of ricevar. Ciherang and Inpari 13. This study used a completelyrandomized design with gamma irradiation treatment atdoses of 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 Gy. Eachtreatment consisted of 10 replicates with 5 embryogenic calliper bottle. The results showed that the increasing doses ofgamma irradiation increased the percentage of dead callus,inhibited callus growth, and its ability to regenerate. Thehigh percentages of callus of Ciherang and Inpari 13 thatformed green spots and adventitious shoots were mostlyobtained from controls. The percentages decreased atirradiation doses of 10, 20, 30, and 40 Gy. Moreover, the calliof Ciherang and Inpari 13 were not able to form adventitiousshoots at irradiation doses higher than 40 Gy and 30 Gy,respectively.
POTENSI BAKTERI ENDOFIT DALAM UPAYA MENINGKATKAN PERTUMBUHAN, PRODUKSI, DAN KANDUNGAN ANDROGRAFOLID PADA TANAMAN SAMBILOTO GUSMAINI GUSMAINI; SANDRA ARIFIN AZIZ; ABDUL MUNIF; DIDY SOPANDIE; NURLIANI BERMAWIE
Jurnal Penelitian Tanaman Industri Vol 19, No 4 (2013): Desember 2013
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v19n4.2013.167-177

Abstract

ABSTRAKBakteri endofit hidup di dalam jaringan tanaman yang sehat danberperan antara lain di dalam memacu pertumbuhan tanaman denganmenghasilkan senyawa-senyawa zat pengatur tumbuh, seperti IAA, GA 3 ,dan Sitokinin. Tujuan penelitian ini untuk mengevaluasi potensi bakteriendofit dalam  meningkatkan pertumbuhan, produksi, dan kadarandrografolid pada tanaman sambiloto. Penelitian dilakukan di rumah kacaBalittro Cimanggu Bogor pada Oktober 2011–Mei 2012. Perlakuandisusun mengikuti Rancangan Acak Kelompok, enam perlakuan danempat ulangan. Perlakuan terdiri dari (1) kontrol, dan perlakuan bakteriendofit yaitu (2) 20BB, (3) 5MD, (4) 20BD, (5) 20CD (perlakuan 2-5masing-masing terdiri dari 4 jenis isolat), dan (6) 90AA (isolat tunggal).Suspensi bakteri endofit (50 ml/tanaman) diberikan 4 kali yaitu padaminggu ke 3, 5, 7, dan 9 setelah tanam dengan konsentrasi 10 10 spk/ml.Hasil penelitian menunjukkan bahwa bakteri endofit berpengaruh postifdan nyata dalam meningkatkan pertumbuhan, produksi herba segar dankering serta andrografolid pada tanaman sambiloto lebih baikdibandingkan kontrol. Peningkatan pertumbuhan tertinggi ditunjukkanpada tinggi tanaman dan jumlah cabang primer yaitu masing-masing24,7% (20 CD) dan 42,2% (20 BB). Produksi herba kering meningkat 25-82,81%, sejalan dengan meningkatnya serapan hara N (64,7-158,8%), P(50-100%), dan K (65-155%). Peningkatan produksi herba kering danandrografolid terbaik diperoleh dari penggunaan 20 CD (82,81 dan142,11%), 20 BB (88,75 dan 131,58%), dan 20 BD (65,63 dan 131,58%).Implikasi dari hasil penelitian ini bahwa bakteri endofit berpotensi untukdikembangkan pada budidaya tanaman sambiloto.Kata kunci: Andrographis paniculata, bakteri endofit, andrografolid,pertumbuhan, produksiABSTRACTEndophytic bacteria live within healthy plant tissue and playimportant roles, such as producing compounds of plant growth regulatorssubstances such as IAA, GA 3 , and Cytokinin. The aims of this research isto evaluate the potential of endophytic bacteria to promote the growth,andrographolide content, and dry matter yield of king of bitter. Theresearch was conducted in the greenhouse of Cimanggu Balittro in October2011-May 2012. Treatments were arranged in a randomized completeblock design with six treatments and four replications. Treatments consistof (1) control, and 5 kinds of endophytic bacteria isolates such as (2)20BB, (3) 5MD, (4) 20BD, (5) 20CD (treatments no.2-5, consisted of 4types of isolate), and (6) 90AA (single isolate). The highest presentage ofplant height and number of primary branches were obtained from thetreatment of 20CD (24.7%) and 20BB (42.2%). Increase in the dry herbyield of 25-82.81% was in agreement with increasing in uptake of N (64.7-158.8%), P (50-100%), and K (65-155%). The best treatment with whichyielding high of dry herbs and andrographolide was 20CD isolates (82.81and 142.11%), followed with 20 BB (88.75 and 131.58%), and 20 BD(65.63 and 131.58%). The study implies that endophytic bacteria havepotential for development of king of bitter cultivation.Key words: Andrographis paniculata, endophytic bacteria, androgra-pholide, growth, yield
PEMANFAATAN BAKTERI ENDOFIT DAN FOSFAT UNTUK PERTUMBUHAN DAN PRODUKSI TANAMAN SAMBILOTO / Utilizing Endophytic Bacteria and Phosphate for Growth and Yieald of Andrographis paniculata Gusmaini Gusmaini; Didy Sopandie; Sandra Arifin Aziz; Abdul Munif; Nurliani Bermawie
Jurnal Penelitian Tanaman Industri Vol 22, No 3 (2016): September, 2016
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v22n3.2016.153-159

Abstract

Andrographis paniculta produce andrographolide, which have functions as raw material for medicine. One can stimulate andrographolide yield by utilizing endophytic bacteria and phosphate. This research objectives were to obtain effect of endophytic bacteria and phosphate on growth, yield and andrographolide content, N, P, and K uptake of A. paniculta. The research was conducted at the experimental garden of ISMCRI, at Cimanggu, Bogor from June 2012-February 2013. The trial was arranged in Random Block Design, factorial, 9 treatments and 3 replications. The first factor were endophytic bacteria; 1) without endophytic bacteria, 2) 20CD, and 3) 20BB. The second factor were the levels of P; 1) without P, 2) 27 kg/ha P, and 3) 54 kg/ha P. The results showed that Endophytic bacteria application and P fertilizer significantly increased plant growth, dry matter yield, andrographolide content and yield, and N, P, K uptake, but there were no interaction between endophytic bacteria and P treatments. Giving of endophytic bacteria improved content and yield of andrographolide ranging 16,9-29,9% and 37,6-45,7% respectively. A dossage of 27 kg/ha P was recommended to produce dry matter and andrographolide yield, and andrographolide content obtained 2.56%. The best andrographolide yield should be harvested at the beginning of generative phase.Keywords: Andrographis paniculata, andrographolide content, endophytic bacteria, phosphate, yield. AbstrakTanaman sambiloto merupakan tanaman penghasil andrografolid, yang berfungsi sebagai bahan baku obat. Salah satu yang dapat memacu produksi andrographolid tersebut dengan memanfaatkan bakteri endofit dan fosfat. Penelitian bertujuan untuk mengetahui pengaruh bakteri endofit dan fosfat terhadap pertumbuhan, produksi dan kadar andrografolid serta serapan hara N, P, dan K pada tanaman sambiloto. Penelitian dilaksanakan di Kebun Percobaan Cimanggu Balai Penelitian Tanaman Rempah dan Obat (Balittro), Bogor dari bulan Juni 2012-Februari 2013. Penelitian ini menggunakan rancangan acak kelompok, faktorial, 9 perlakuan dan 3 ulangan. Faktor pertama yaitu bakteri endofit; 1) tanpa bakteri, 2) 20CD, dan 3) 20BB. Faktor kedua adalah dosis pupuk P; 1) tanpa pupuk, 2) 27 kg/ha P, dan 3) 54 kg/ha P. Hasil penelitian menunjukkan bahwa bakteri endofit dan P nyata meningkatkan pertumbuhan, produksi biomas, kadar dan produksi andrografolid, serta serapan hara N, P, dan K pada umur tanaman 14 MST, namun tidak terdapat interaksi antara kedua faktor tersebut. Pemberian bakteri endofit dapat meningkatkan kadar dan produksi andrografolid, masing-masing berkisar 16,9-29,9% dan 37,6- 45,7%. Dosis 27 kg/ha P direkomendasikan untuk menghasilkan produksi bahan kering dan produksi andrografolid, dengan kadar andrografolid yang diperoleh 2,56%. Produksi andrografolid terbaik, tanaman dipanen pada fase awal generatif.Kata kunci: Andrographis paniculata, andrografolid, bakteri endofit, pertumbuhan, fosfat, produksi.
Comparative Analysis of Rice Transformation Using Agrobacterium tumefaciens and Rhyzobium leguminosarum Syamsidah Rahmawati; Osmat Azzam Jefferson; Didy Sopandie; S. Suharsono; Inez Hortense Slamet-Loedin
Indonesian Journal of Biotechnology Vol 15, No 1 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (287.394 KB) | DOI: 10.22146/ijbiotech.7821

Abstract

This study was aimed to study the effectiveness of Rhizobium transformation system compared to the most widely used Agrobacterium mediated transformation system on three rice cultivars, Ciherang (Indica), Nipponbare (Japonica), and Rojolele (Javanica). Six day old calli induced from immature embryos were inoculated with Rhizobium leguminosarum bv trifolii ANU845 and Agrobacterium tumefaciens LBA288 that harbored with vector pCAMBIA 5106. This plasmid contained a minimum set of transfer machinery genes and had a gusplus and an hptII gene driven by 35S CaMV promoter in the T-DNA. The results showed that the transformation frequencies (number of PCR positive plants per number of calli inoculated) ranging from 0 to 12.05 % depend on the genotype and transfer agent used. The highest transformation frequency (12.05%) was obtained in Ciherang transformed with R. leguminosarum. Most of the transgenic rice obtainedby Rhizobium transformation were normal in morphology and fertile similar to those obtained by Agrobacterium transformation. Integration, expression and inheritance of transgenes were demonstrated by molecular and genetic analysis in T0 and T1 generations.Key words : Rhizobium leguminosarum, immature embryos, Agrobacterium tumefaciens
Co-Authors , Hamin , Kisman , Purwono , Suharsono , Syafruddin , Tasliah . SUHARSONO . SUWARNO A. HAITAMI A.A. Ketut Agung Cahyawan W Abd. Aziz Syarif ABDUL KARIM MAKARIM Abdul Munif Abdul Qadir Adolf Pieter Lontoh, Adolf Pieter Agus Purwito AHMAD JUNAEDI AKIHO YOKOTA AKIHO YOKOTA Akiho Yokota Aksarah Pas, Aris Alce Ilona Noyaa Alifiya Herwitarahman Amris Makmur Amrullah Amrullah Anas D Susila Anas Dinurrohman Susila ANGELITA PUJI LESTARI Angelita Puji Lestari Annisa Fadila Ardie, and Sintho Wahyuning Ardie, dan Sintho Wahyuning Arvita Netti Sihaloho, Arvita Netti ASMARLAILI SAHAR Atang Sutandi Azri Kusuma Dewi Azri Kusuma Dewi Budi Mulyanto Careca Sepdihan Rahmat Hidayatullah Chairani Hanum Chairani Hanum Christoph Leuschner Deden Derajat Matra Desta Wirnas Dhika Prita Hapsari DWI ANDREAS SANTOSA Edi Santosa Edi Santosa Edi Santosa Efendi, Darda Enny Sudarmonowati Enny Sudarmonowati Enung Sri Mulyaningsih Enung Sri Mulyaningsih Eny Widajati Erma Prihastanti Fadliah Salim Faqih Udin Gusmaini Gusmaini GUSMAINI GUSMAINI GUSMAINI GUSMAINI, GUSMAINI HAJRIAL ASWIDINNOOR Hajrial Aswidinoor Halimatus Syahdia Hasibuan Hamim Hamim Hanedi Darmasetiawan HANNA ARTUTI EKAMAWANTI Hapsoh Hidayah, Agus Nur I Ketut Suada IBNUL QAYIM Ida Hanarida Soemantri Ika Mariska Ika Mariska Ika Mariska Ilona Noyaa, Alce Imam Widodo Inez Hortense Slamet Loedin Inez Hortense Slamet Loedin Inez Hortense Slamet-Loedin INEZ HORTENSE SLAMET-LOEDIN Iskandar Lubis Joko Prasetiyono Joko Prasetiyono Juang Gema Kartika K. Idris Karlin Agustina Kartika Ning Tyas Ketty Suketi KINYA AKASHI KINYA AKASHI Kisman Kisman Komaruddin Idris Komaruddin Idris La Muhuria Laela Sari Laela Sari laela Sari, laela Lukman Chakim Luluk Setyaningsih Lutfy Ditya Cahyanti, Lutfy Ditya M A Chozin M. A. Chozin Maemonah, Maemonah Mahpuzah, Marfiatun Mariska, Ika Masdiar Bustamam Masdiar Bustamam Masumi Moritsugu Matra, Deden Derajat Maya Melati Mayang Sari Meliala, Merry Gloria Moh. Yani Momongan, Jorex Daniel Muhamad Syukur Muhammad Arif Yudiarto Muhammad Jusuf Munarti Munif Ghulamahdi Muzuni, Muzuni Nani Heryani Nasution, Annisa Andrini Ni Made Armini Wiendi Nurliani Bermawie Nurliani Bermawie NURLIANI BERMAWIE Nurul Khumaida Osmat Azzam Jefferson Osmat Azzam Jefferson, Osmat Azzam OuwerkerV, Pieter BF Pieter B.F. Ouwerkerk Pieter BF OuwerkerV Putri Andini Mandasari RADITE TISTAMA Ragapadmi Purnamaningsih RAGAPADMI PURNAMANINGSIH Ratna Yuniati Rini, Erin Puspita Roedhy Poerwanto Rossa Yunita Rossa Yunita Rossa Yunita Rosyad, Astryani S. Suharsono Saepudin, Adam Sandi, Yusri Iryas Sandra Arifin Aziz Saragih, Ery Leonardo Sari, Laela Sarsidi Sastrosumarjo SATRIYAS ILYAS Satya Nugroho Sinaga, Falencia Sinaga, Parlin H. Sintho Wahyuning Ardie Siti Marwiyah Slamet Loedin, Inez Hortense Sobir Sobir SOEKISMAN TJITROSEMITO Soeranto Human Sri Mulatsih SRI RAHAYU Sri Setyati Harjadi Sri Wilarso Budi Sudarmonowati, Enny Sudirman Yahya Sudirman Yahya Sugianta Sugianta Sugianta, Sugianta SUGIONO MOELJOPAWIRO Sugiono Moeljopawiro Suharsono . Suharsono Suharsono Suharsono Suharsono Suhartanto, Muhammad Rahmad Sumiati, dan Sungkono Sungkono Supijatno Surjono Hadi Sutjahjo Suwarno Suwarno Suwarno Suwarno Suwarto Suwarto Syamsidah Rahmawati Syamsidah Rahmawati, Syamsidah T. Kawasaki T. M. H. Oelim Tanari, Yulinda Tintin Suhartini Titin Handayani Toshio Kawasaki Toyip Toyip TRI KOESOEMANINGTYAS Tri Lestari Trikoesoemaningtyas Utami, Nita Nur UTUT WIDYASTUTI UTUT WIDYASTUTI Utut Widyastuti Utut Widyastuti Suharsono Wage Ratna Rohaeni Wahju Q. Mugnisjah Wahju Qamara Mugnisjah Yadi Setiadi Yadi Setiadi Yadi Setiadi YADI SETIADI Yonny Koesmaryono Yopie Moelyohadi Yuli Sulistyowati YULIN LESTARI Yuniati, Ratna Yunita, Rossa