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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA MEDIA KONSERVASI Jurnal Primatologi Indonesia Hemera Zoa Forum Pasca Sarjana Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner Microbiology Indonesia Jurnal Ilmu dan Teknologi Hasil Ternak Jurnal Penelitian Hasil Hutan Jurnal Teknologi Pangan agriTECH JURNAL BIOLOGI INDONESIA The Indonesian Biomedical Journal Jurnal Aplikasi Teknologi Pangan JSFK (Jurnal Sains Farmasi & Klinis) Jurnal Psikologi Sosial Jurnal Respirologi Indonesia JURNAL KAJIAN VETERINER ZOOTEC Makara Journal of Science The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy Jurnal Kedokteran Hewan Jurnal Penelitian Hasil Hutan

Dondin Sajuthi

Departemen Klinik, Reproduksi, Dan Patologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor Jl. Raya Dramaga, Babakan, Dramaga, Bogor, Jawa Barat, Indonesia 16680

Author-ID : 255503

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Pengembangan Metode Identifikasi Kerusakan DNA Spermatozoa Ternak Teguh Ari Prabowo; R. Iis Arifiantini; Dondin Sajuthi; Uus Saefullah
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The success of artificial insemination is very much determined by the quality of spermatozoa. The detection or identification of damaged chromatin of spermatozoa DNA is very important to forsee the adverse clinical outcome. However, the method of identification is still depended on expensive imported kits. Therefore, the objective of this research was to developed an identification kit to determine the quality of livestock spermatozoa DNA chromatin.This study consist of three step. Step 1) Determination of low melting point agarose (LMP-agarose) concentration which is 0,6%, 0,7% and 0,8%. 2) Comparison of three lysis solution (LS) which is LS I (0.4M Tris, 0.8M DTT, 1% SDS, pH 7.5), LS II (0.4M Tris,2 M NaCl, 1% SDS , pH 7.5), and LS III (0.4M Tris-HCl, 2M NaCl, 1% SDS 0,05 M EDTA, pH 7.5). 3) Comparison different staining which is Eosin yellow and Methylene blue. The results showed that 0.6% LMP-agarose demonstrated the best concentration to “trapped the spermatozoa” compared for sheep and goats. whereas the three concentration of spermatozoa cows can not be used to trap spermatozoa cow. The best formulation to lysis the membrane was LS III (0.4M Tris -HCl, 2M NaCl, 1% SDS 0,05 M EDTA). The best staining was eosin yellow and methylene blue with 2:1 ratio.

Lipid oxidation and antimicrobial activity of cooked beef patties as influenced by leaf extracts of “Cemba” (Albizia lebbeckoides [DC.] Benth) Hajrawati Hajrawati; Henny Nuraini; Irma Isnafia Arief; Dondin Sajuthi
Buletin Peternakan Vol 43, No 1 (2019): BULETIN PETERNAKAN VOL. 43 (1) FEBRUARY 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i1.38517

Cemba (Albizia lebbeckoides [DC.] Benth.) leaf extract (CLE) was evaluated for some physical properties, antioxidant and antimicrobial activities incorporated into beef patties during cold storage. Four Formulation employed were control, butylated hydroxytoluene (BHT) 0.01, CLE 0.5, and CLE 1% (w/w). The variables measured were proximate composition, cooking parameters, pH, aw, WHC, color, total phenolic content, antioxidant capacity, DPPH scavenging activity, TBARS value, and microbial total. The data were analyzed using ANOVA one factor for proximate and cooking parameters, and ANOVA with factorial 4x5 for pH, aw, WHC, color, total phenolic content, antioxidant capacity, DPPH scavenging activity, TBARS value, and microbial total and continued with Tukey test. The results of the study showed that the addition of the CLE did not affect the proximate composition and cooking parameters of the patties. The cooked beef patties with 1% CLE showed significantly lower (P<0.05) for TBARS value, pH, bacterial total (mesophilic and psychrophilic) compared to 0.5% CLE and controls. The total phenolic content, antioxidant capacity, scavenging activity of CLE 1 % were significantly higher (P<0.05) than 0.5% CLE and controls during the cold storage period (0, 7, 14, 21, and 28 days). Addition of both 0.5 and 1% CLE in cooked beef patty reduced bacteria total. The addition of 1% CLE had equivalent to BHT 0.01% effect in retarding lipid oxidation. In conclusion, the CLE 1% was effective to retard lipid oxidation and inhibit bacteria growth of cooked beef patties.

Variasi Molekuler Gen Reseptor Melanokortin-4 pada Monyet Ekor Panjang I Gusti Agung Arta Putra; Dondin Sajuthi; Dedy Duryadi Solihin; Raden Roro Dyah Perwitasari
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (124.565 KB)

Melanocortin-4 receptor (MC4R) is one of G protein-coupled receptors that plays an important role inregulation of energy homeostasis. MC4R mutations constitute the most common cause of human obesity.The study was conducted in order to investigate the variation of MC4R gene in Macaca fascicularis and itsassociation with obesity as a model of human obesity. Forty eight adult male macaques from Bali (Ubudand Uluwatu), East Java (Alas Purwo and Baluran), and Sumatera island (Palembang) were used in thisresearch. The animals had been anaesthetized using ketamine (10 mg/kg body weight) and xylazine (2mg/kg body weight) before collecting blood samples and phenotypic data (weight, crown rump length. Bloodsamples were used as source of DNA. To determine MC4R variation, coding region of this gene wasamplified and sequenced. The results showed that 20 variations sites were identified and 13 of them werenon-synonymous. Among the non-synonymous mutations, five mutations were only found in obese macaque;two mutations were found both in obese and non-obese macaque; and six mutations were only found in nonobesemacaque .

Konsentrasi Protein Total, Albumin, dan Globulin Anak Kambing Peranakan Etawah Setelah Pemberian Berbagai Sediaan Kolostrum* (TOTAL PROTEIN, ALBUMIN, AND GLOBULIN CONCENTRATIONS ON ETTAWAH CROSSBREED NEONATES FOLLOWING THE ADMINISTRATION OF VARIOUS FORM O Anita Esfandiari; Sus Derthi Widhyari; Setyo Widodo; I Wayan Teguh Wibawan; Dondin Sajuthi; I Ketut Sutama
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (140.331 KB)

This experiment was conducted to study the profile of total protein, albumin, and globulin concentrationson Ettawah crossbreed neonates after consuming various colostrums. Twenty four healthy neonatal kidswere used in this study. The neonates were divided into four groups. Each group received fresh maternal(goat) colostrum, frozen-thawed bovine colostrum, bovine spray dried colostrum, and bovine powdercommercial colostrum, respectively. Colostrums were given at 10% of body weight directly after birth andfollowed by the same amount every 12 hours, for three days. The blood was taken from jugular vein at 0, 12,24, 48, 72, and 168 hours after birth to determine total protein, albumin, and globulin concentrations.Results of this study indicated that the serum total protein and globulin concentration increased andreached the peak at 24 hours after birth. Compared to the concentration at birth, the increase of totalprotein concentration were 62.77%, 59.26%, 48.05%, and 66.67% in fresh maternal (goat), frozen-thawedbovine, bovine spray dried, and commercial bovine colostrum, respectively. Serum globulin concentrationincreased 4.9, 4.4, 4.8, and 14.6 times in fresh matermnal goat, frozen-thawed bovine, spray dried, andcommercial bovine colostrums respectively, compared to the concentration at birth. In conclusion, theconsumption of various colostrums i.e. fresh maternal goat colostrums, bovine colostrums (frozen-thawed,spray dried and commercial colostrums) would increase the concentration of blood total protein and globulin,which both reached the highest concentration at 24 h after birth.

Variasi Kolesterol Plasma Individual Monyet Ekor Panjang (Macaca fascicularis) sebagai Respons terhadap Diet Aterogenik IPB-1 [INDIVIDUAL PLASMA CHOLESTEROL VARIATION OF CYNOMOLGUS MACAQUE (MACACA FASCICULARIS) IN RESPONSE TO IPB-1 ATHEROGENIC DIET] Achmad Taher; Dedy Duryadi Solihin; Sulistiyani Sulistiyani; Dondin Sajuthi; Dewi Apri Astuti
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The effectiveness of atherogenic diet in raising plasma cholesterol level of cynomolgus macaques(Macaca fascicularis) can be predicted for population, but not for individuals.This study aimed to evaluatethe individual plasma cholesterol of cynomolgus macaques in response to IPB-1 atherogenic diet. Theanimals under study were 22 adult malemonkeys from the animal facility of Primate Research CenterBogor Agricultural University (PSSP IPB). All animals were intervened with the IPB-1 atherogenic diet forthree months and individual plasma cholesterol wasevaluated in a monthly basis.The results showedthat the monkeys’ plasma cholesterol had increased significantly (P <0.05) after one month of interventionperiod and the increases were very significantly different (P <0.01) after three months. Individually, increasesin plasma cholesterol varied among animals. Based on these variations, 2 animals were categorised ashypo-response, 18 animals as hyper-response and 2 animals as extreme.This study showed the variationof individual plasma cholesterol of cynomolgus macaques in response tothe IPB-1 atherogenic diet so thatthe animals might be selected based on the their responsiveness.

Kualitas Semen Cair Kambing Peranakan Etawah dalam Modifikasi Pengencer Tris dengan Trehalosa dan Rafinosa (THE QUALITY OF ETAWAH CROSSBREED BUCK LIQUID SEMEN IN MODIFIED TRIS DILUENTS WITH TREHALOSE AND RAFFINOSE) Oriza Savitri Ariantie; Tuty Laswardi Yusuf; Dondin Sajuthi; Raden Iis Arifiantini
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The aim of this study was to compare the efficacy of trehalose and raffinose supplementation in Trisegg yolk (TEY) and Tris soya (TS) diluents in optimizing the quality of Etawah Crossbreed liquid semen.Semen were collected from three sexually mature bucks using artificial vagina. Semen were then evaluatedand divided into six aliquot tubes. Each of them was diluted in TEY and TS supplemented with 50 mMtrehalose or raffinose, respectively. The liquid semen were then stored in refrigerator (5°C). The motility,viability and plasma membrane integrity (PMI) of the spermatozoa were evaluated every 12 hours untilsperm motility remained up to 50%. The results showed that sperm motility in TEY supplemented withtrehalose or raffinose remained up to 50% for 72-84 hours, compared to in TS which was for 48-60 hours(P<0,05). The best diluent was demonstrated by TEY supplemented with trehalose where the spermmotility was 52,82±3,21% up to 84 h compared to raffinose supplementation (52,78±4,41%) and control(51,78±4,86%) which was up to 72 hours, respectively. Meanwhile, the spermatozoa motility in TS diluentsupplemented with raffinose was 52,78±4,41% for up to 60 hours compared to supplemented with trehalose(53,33±3,54%) and control (51,11±4,86%) which was up to 48 hours. In all diluents, the viability ofspermatozoa was 6-9% higher than the percentage of sperm motility, whilst the percentage of PMI wassimilar to the percentage of sperm motility. In conclusion, TEY supplemented with trehalose was the bestdiluents for preservation of Etawah Crossbreed buck liquid semen, and when using TS diluent it isrecommended to add raffinose rather than trehalose.

PRODUCTION OF EMBBRYONIC STEM CELLS FROM INNER CELL MASS OF BLASTOCYST ISOLATED BY ENZYMATIC AND IMMUNOSURGERY METHODS Thomas Mata Hine; Iman Supriatna; Dondin Sajuthi; Arief Boediono
Jurnal Veteriner Vol 9 No 1 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The objective of the research is determining the ICM isolation method to produce ESC. Blastocyst stage of DDy mice embryos were used in this study. Zona pellucida of blastocysts were removed by 0.25% pronase, the ICM isolation were done by enzimatic or immunosurgery method, and then they were cultured in DMEM-high glucose supplemented with mercaptoethanol, gentamycin, fetal bovine serum, and cumulus cells as feeder layer. The result of the research indicated that immunosurgery method yielding attachment rate and number ESC colony 93.85% and 43.08%, respectively, higher (P<0.05) than enzimatic method that weree 79.63% and 18.52%, respectively, but the viability of ICM cells were equal (P >0.05) that are 93.59% in enzymatic method and 98.56% in immunosurgery method. This research concluded that immunosurgery more effective method for isolation of ICM and ESC production than enzymatic method.

Prevalensi dan Kepekaan Bakteri Enteropatogen terhadap Antibiotik pada Monyet Ekor Panjang dengan Diare di Fasilitas Penangkaran Institut Pertanian Bogor di Dramaga Fhady Risckhy Loe; Suzy Tomongo; Dondin Sajuthi; Uus Saepuloh; Dondin Sajuthi; Irma H Suparto
Jurnal Veteriner Vol 22 No 4 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Monyet ekor panjang (Macaca fascicularis) adalah salah satu hewan laboratorium dalam penelitian biomedis. Fasilitas penangkaran untuk hewan laboratorium diatur dan didukung oleh kaidah kesejahteraan hewan dan manajemen kesehatan yang baik. Salah satu masalah yang sering terjadi di fasilitas penangkaran adalah diare, termasuk di fasilitas penangkaran IPB Dramaga. Diare menyebabkan penurunan produktivitas karena tingginya morbiditas dan mortalitas. Bakteri enteropatogenik adalah salah satu penyebab diare yang dapat ditularkan antar hewan juga melalui air dan pakan. Oleh karena itu, tujuan dari penelitian ini adalah untuk mengidentifikasi prevalensi bakteri enteropatogen pada sampel feses, air dan pakan, menguji kepekanan bakteri enteropatogen terhadap antibiotik dari sampel feses di fasilitas penangkaran Dramaga. Sebanyak 30 sampel feses, enam sampel air dan dua sampel pakan dikoleksi kemudian diuji untuk mengetahui adanya bakteri enteropatogen. Metode mikrobiologis standar digunakan untuk mengisolasi dan mengidentifikasi bakteri enteropatogen dari sampel feses dan pengujian kepekaan antibiotik.. Air dan pakan diuji menggunakan metode angka paling mungkin. Hasil isolasi dan identifikasi bakteri dari sampel feses adalah E. coli (100%), Salmonella enteritidis (97%) dan Shigella sp. (60%). Kontaminasi E. coli dan coliform dalam sampel air dan pakan (pisang dan monkey chow) terdeteksi di atas batas minimum (0/100 mL/g). Semua bakteri enteropatogen sensitif terhadap siprofloksasin dan gentamisin tetapi resisten terhadap eritromisin.

Perubahan Patologi Seluler Kelenjar Adrenal Tikus Hipertensi dengan Terapi Sel Punca Mesenkimal Sumsum Tulang (CELLULER STUDY OF ADRENAL GLAND IN HYPERTENSIVE RATS WITH IMPACT OF BONE MARROW MESENCHYMAL STEM CELLS THERAPY) Yanse Yanne Rumlaklak; Erni Sulistiawati; Dondin Sajuthi; Sus Derthi Widhyari; Setyo Widi Nugroho
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The purpose of this study was to investigate changes in cellular pathology of adrenal gland as impact of bone marrow mesenchymal stem cells (BMMSC) therapy in hypertensive rats. The research used ten male wistar rats with age ± 10-12 weeks with body weight ± 200-250 grams which is devided into two different groups ie BMMSc(-) hyprtention and BMMSc(+) hypertension. Rats are conditioned with hypertention using Hasbinoto et al method. Right nephrectomy and left carotid communic artery ligation are performed on all rats. Rats are injected with deoxycorticosterone acetate (DOCA), then 0.12 % of âaminopropionitrile fumarate (BAPN) is added into drinking water. 1% NaCl solution was administered as drinking water during the study. Rats were evaluated for particular indicator ie blood pressure then were euthanized for adrenal organs collections. The data that obtained then analysed using qualitative descriptive. The result of the study indicate hypertention therapy using BMMSC can repair cell damaged of the adrenal gland such as hyperplasia, nodules, cysts and cytoplasm vacuolization.

Co-Authors . Permanawati Achmad - Taher Achmad Taher Achmad Taher Achmad, Taher Adi Winarto Agus Harsoyo Agus Harsoyo Agus Lelana Agus Wijaya Agus Wijaya Ahmad Kamal Nasution Alexander Nater Anita Esfandiari Annisa Dewi, Fitriya Nur Antonius Suwanto Anwar Wardy Warongan Arief Boediono Aryo Tedjo Bambang Purwantara Bayu Febram Prasetyo Bayu Rosadi Budiman Bela C Hanny Wijaya C. Hany Wijaya Cecep Kusmana CHAIRIN N MAROEF Chusnul Choliq Chusnul Choliq Chusnul Choliq Cucu K Sajuthi Dadang Makmun Daldiyono Daldiyono Daldiyono Daldiyono Dedi Rahmat Setiadi DEWI APRI ASTUTI Dewi, Fitriya Nur Annisa Diah Iskandriati Diah Iskandriati Diah Iskandriati Diah Iskandriati Diah Iskandriati DIAH ISKANDRIATI Diah Iskandriati DIAH ISKANDRIATI Douglas M. Bowden Dwi Atmoko Agung Nugroho DYAH PERWITASARI -FARAJALLAH Ekowati Handharyani Entang Iskandar Entang Iskandar Entang Iskandar Erdiansyah Rahmi Eric Hayes ERIC HAYES Eric Hayes ERLIN LISTIYANINGSIH Erni Sulistiawati ESTHER ARIFIN Fauzi Abdurrahman Munggaran Fhady Risckhy Loe Fransiska R Zakaria FUNGKEY HOETAMA Gede Eko Darmono Gregory K. Wilkerson Hadi S Alikodra Hajrawati Hajrawati Harrold Halilintar MR Subu Taopan Henny Nuraini HERA MAHESHWARI Huda S Darusman Huda Shalahuddin Darusman Huda Shalahudin Darusman I Gusti Agung Arta Putra I Gusti Ngurah Sudisma I Ketut Sutama I NENGAH BUDIARSA I Nengah Budiarsa I Nengah Budiarsa I wayan Teguh Wibawan IETJE WIENTARSIH Iis Afriantini Iman Rusmana Iman Supriatna IMELDA WINOTO Indra Bachtiar Indra Bachtiar Irawan Jusuf Irma H Suparto Irma H Suparto Irma H Suparto Irma Herawati Suparto Irma Isnafia Arief Irma Suparto J. Mark Cline Jansen Manansang Jansen Manansang Jayusman Arsiyanti Joesoef JONATHAN STURGIS Kanti Rahmi Fauziyah Kartika Sari KATHARINA JESSICA KEVIN RANDALL PORTER Koeswinarning Sigit Latifah K. Darusman Latifah K. Darusman Leni Maylina Lina Elfita Lina Elfita Luluk Lailatul Hasanah LUTHFIRALDA SJAHFIRDI Luthfiralda Sjahfirji M Agus Setiadi MARYATI SURYA Maxs Urias Ebenheizer Sanam Michael Krützen MOSES AFFANDI Mozes R. Tolihere Muchidin Noordin MUHAMMAD AGIL Nanik Hidayatik Nur Hidayati Nurhidayat . Nurul Oktaviani Oktaviani, Nurul Oriza Savitri Ariantie Paini Sri Widyati Pamungkas, Joko PATRICK J BLAIR Peni Harjosworo PENI SUPRAPTI HARDJOSWORO Permanawati Permanawati PERMANAWATI PERMANAWATI Permanawati Permanawati PERMANAWATI, PERMANAWATI Pijoh, Deyv Praira, Willy Pudji Astuti PUDJI ASTUTI Pudji Astuti Puji Rianti Puspita Sari Putri, Zulfitra Utami R. Iis Arifiantini R.P Agus Lelana RATNA TAN Retno Wulansari Reviany Widjajakusuma Reviany Widjajakusuma Ria Oktarina RICHARD F GRANT Rini Widayanti Rumlaklak, Yanse Yane Sajuthi, Cucu K Sela S Mariya Sela S. Mariya SELA SEPTIMA MARIYA Sela Septima Mariya Septi Iriani Setyaningsih Rambu Liwa Setyaningsih Rambu Liwa Setyo Widi Nugroho Setyo Widodo Silmi Mariya Silmi Mariya SILMI MARIYA SILMI MARIYA Silmi Mariya Silmi Mariya Silvia Triwidyaningtyas Sony Hartono Wijaya Sri Rahmatul Laila Sri Soepraptini Mansjoer Sri Supraptini Mansjoer Srihadi Agungpriyono Subu Taopan, Harrold Halilintar MR Suharyanto Suharyanto Suharyanto Suharyanto Sulistiyani Sulistiyani . SULISTIYANI SULISTIYANI Sulistiyani Sulistiyani SULISTIYANI SULISTIYANI Sulistiyani, Sulistiyani Suparto, Irma Sus Derthi Widhyai Sus Derthi Widhyari SUSAN SOKA SUSANA WIDJAJA Suzy Tomongo Taher, Achmad TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Teguh Ari Prabowo Teguh Budipitojo Tejasari . Thomas Mata Hine Tiara Widyaputri Tri Wahyu Pangestiningsih Trini i Susmiat Trini Susmiati Tuti Suryati Tuty L Yusuf Tuty Laswardi Yusuf Tuty Laswardy Yusuf Unang Supratman Uus Saefullah Uus Saepuloh Uus Saepuloh Uus Saepuloh Uus Saepuloh UUS SAEPULOH UUS SAEPULOH Vetnizah Juniantito Villiandra Villiandra Villiandra Villiandra Villiandra Villiandra Vincentia Trisna Yoelinda Willy Praira Yanse Yanne Rumlaklak Yanti . Yanti Yanti Yasmina A Pramastri Yasmina A. Paramastri Yasmina Arditi Pramastri YASMINA PARAMASTRI Yoga Yuniadi Yohana Tri Hastuti Yohana Tri Hastuti Yohana Tri Hastuti Yuli Purwandari Kristiangingrum Yundari, Yundari Zulfitra Utami Putri Zulfitra Utami Putri Zuraida Zuraida Zuraida

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