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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA MEDIA KONSERVASI Jurnal Primatologi Indonesia Hemera Zoa Forum Pasca Sarjana Jurnal Sain Veteriner Buletin Peternakan Microbiology Indonesia Jurnal Ilmu dan Teknologi Hasil Ternak Jurnal Penelitian Hasil Hutan Jurnal Teknologi Pangan agriTECH JURNAL BIOLOGI INDONESIA The Indonesian Biomedical Journal Jurnal Aplikasi Teknologi Pangan JSFK (Jurnal Sains Farmasi & Klinis) Jurnal Psikologi Sosial Jurnal Respirologi Indonesia JURNAL KAJIAN VETERINER ZOOTEC Makara Journal of Science The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy Jurnal Kedokteran Hewan Jurnal Penelitian Hasil Hutan

Dondin Sajuthi

Departemen Klinik, Reproduksi, Dan Patologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor Jl. Raya Dramaga, Babakan, Dramaga, Bogor, Jawa Barat, Indonesia 16680

Author-ID : 255503

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Pengembangan Metode Identifikasi Kerusakan DNA Spermatozoa Ternak Teguh Ari Prabowo; R. Iis Arifiantini; Dondin Sajuthi; Uus Saefullah
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The success of artificial insemination is very much determined by the quality of spermatozoa. The detection or identification of damaged chromatin of spermatozoa DNA is very important to forsee the adverse clinical outcome. However, the method of identification is still depended on expensive imported kits. Therefore, the objective of this research was to developed an identification kit to determine the quality of livestock spermatozoa DNA chromatin.This study consist of three step. Step 1) Determination of low melting point agarose (LMP-agarose) concentration which is 0,6%, 0,7% and 0,8%. 2) Comparison of three lysis solution (LS) which is LS I (0.4M Tris, 0.8M DTT, 1% SDS, pH 7.5), LS II (0.4M Tris,2 M NaCl, 1% SDS , pH 7.5), and LS III (0.4M Tris-HCl, 2M NaCl, 1% SDS 0,05 M EDTA, pH 7.5). 3) Comparison different staining which is Eosin yellow and Methylene blue. The results showed that 0.6% LMP-agarose demonstrated the best concentration to “trapped the spermatozoa” compared for sheep and goats. whereas the three concentration of spermatozoa cows can not be used to trap spermatozoa cow. The best formulation to lysis the membrane was LS III (0.4M Tris -HCl, 2M NaCl, 1% SDS 0,05 M EDTA). The best staining was eosin yellow and methylene blue with 2:1 ratio.

Lipid oxidation and antimicrobial activity of cooked beef patties as influenced by leaf extracts of “Cemba” (Albizia lebbeckoides [DC.] Benth) Hajrawati Hajrawati; Henny Nuraini; Irma Isnafia Arief; Dondin Sajuthi
Buletin Peternakan Vol 43, No 1 (2019): BULETIN PETERNAKAN VOL. 43 (1) FEBRUARY 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i1.38517

Cemba (Albizia lebbeckoides [DC.] Benth.) leaf extract (CLE) was evaluated for some physical properties, antioxidant and antimicrobial activities incorporated into beef patties during cold storage. Four Formulation employed were control, butylated hydroxytoluene (BHT) 0.01, CLE 0.5, and CLE 1% (w/w). The variables measured were proximate composition, cooking parameters, pH, aw, WHC, color, total phenolic content, antioxidant capacity, DPPH scavenging activity, TBARS value, and microbial total. The data were analyzed using ANOVA one factor for proximate and cooking parameters, and ANOVA with factorial 4x5 for pH, aw, WHC, color, total phenolic content, antioxidant capacity, DPPH scavenging activity, TBARS value, and microbial total and continued with Tukey test. The results of the study showed that the addition of the CLE did not affect the proximate composition and cooking parameters of the patties. The cooked beef patties with 1% CLE showed significantly lower (P<0.05) for TBARS value, pH, bacterial total (mesophilic and psychrophilic) compared to 0.5% CLE and controls. The total phenolic content, antioxidant capacity, scavenging activity of CLE 1 % were significantly higher (P<0.05) than 0.5% CLE and controls during the cold storage period (0, 7, 14, 21, and 28 days). Addition of both 0.5 and 1% CLE in cooked beef patty reduced bacteria total. The addition of 1% CLE had equivalent to BHT 0.01% effect in retarding lipid oxidation. In conclusion, the CLE 1% was effective to retard lipid oxidation and inhibit bacteria growth of cooked beef patties.

Macaca nemestrina and Dengue Virus Infectivity: a Potential Model for Evaluating Dengue Vaccine Candidates SUSANA WIDJAJA; IMELDA WINOTO; JONATHAN STURGIS; CHAIRIN N MAROEF; ERLIN LISTIYANINGSIH; RATNA TAN; JOKO PAMUNGKAS; PATRICK J BLAIR; DONDIN SAJUTHI; KEVIN RANDALL PORTER
Microbiology Indonesia Vol. 4 No. 2 (2010): August 2010
Publisher : Indonesian Society for microbiology

Macaca nemestrina has been shown to respond to infectious disease agents, such as HIV, and is more sensitive compared to other species of macaques such as rhesus (M. mulatta) and cynomolgus monkeys (M. fascicularis). To evaluate M. nemestrina for the ability to support dengue (DEN) viremia and serve potentially as an improved model for testing DEN vaccines, a series of experiments were conducted using primary viral isolates from individuals with DEN virus infections. This study shows that M. nemestrina develops consistent, measurable viremia with all four DEN serotypes and produces immune responses sufficient to protect against homologous virus. Anti-dengue antibodies generated after infection are predominately IgG1. This species of monkey therefore appears to be a suitable model for testing DEN virus vaccine candidates.

Isolation and Characterization of Simian Retrovirus Type D from Macaca fascicularis and M. nemestrina in Indonesia DIAH ISKANDRIATI; UUS SAEPULOH; SILMI MARIYA; RICHARD F GRANT; DEDY DURYADI SOLIHIN; DONDIN SAJUTHI; JOKO PAMUNGKAS
Microbiology Indonesia Vol. 4 No. 3 (2010): December 2010
Publisher : Indonesian Society for microbiology

Simian type D retroviruses (SRVs) are one of the causative agents of simian acquired immunodeficiency syndrome (AIDS) in Asian macaques. In the past, SRV isolates from macaques had only been identified at the US primate centers, outside the country of origin and after the animals had been introduced into a new environment. In this study, we report the first isolation, cultivation and molecular characterization of the type D simian retrovirus naturally infecting wild caught macaques in their natural habitats in the country of origin, in this case, Indonesia. When peripheral blood mononuclear cells (PBMC) from Macaca fascicularis (Mf) and M. nemestrina (Mn) were co-cultured with Raji human B-cell line, syncytia were observed microscopically and confirmed by immunofluoresence assay using antibody to SRV-2. Immunoblot analysis of purified Mf-ET1006 from cell culture supernatants demonstrated that the viral core and envelope proteins reacted with rabbit anti-SRV. Sequence analysis of Mf isolates in the viral envelope region revealed high homology to SRV-2 (94-96%). On the other hand, the homologies in the envelope region of Mn isolates were less than 80% to SRV-1, SRV-2, SRV-3 and Mf isolates. This study suggests that the isolate from Mn may be different from any other published SRV isolates.

Dissemination in Pigtailed Macaques after Primary Infection of Dengue-3 Virus JOKO PAMUNGKAS; DIAH ISKANDRIATI; UUS SAEPULOH; MOSES AFFANDI; ESTHER ARIFIN; YASMINA PARAMASTRI; FITRIYA NUR ANISA DEWI; DONDIN SAJUTHI
Microbiology Indonesia Vol. 5 No. 2 (2011): June 2011
Publisher : Indonesian Society for microbiology

Nonhuman primates (NHPs) play as indispensable animal model in biomedical research for studying a variety of human health issues, diseases and disorders, therapies, and preventive strategies. Since the immunological and physiological responses of NHPs, at some extent, to experimental viral infections are similar to humans, it is possible that studies of dengue infection in NHPs may aid understanding of dengue infection in humans. In this study,we used pigtailed macaques (Macaca nemestrina) as the experimental animal to study dengue-3 (DEN-3) virus infection.We evaluated DEN-3 viral distribution and replication sites after a primary infection in all collected tissues. Sequential localization in tissue of DEN-3 virus was studied in pigtailed macaques euthanized three days post viral inoculation (10 pfu mL ). Pigtailed macaque that was inoculated subcutaneously or intravenously; showed the highest viremia (62.94 pfu mL and 58.62 pfu mL ) detected by one step reverse transcription real time PCR. The virus inoculated in pigtailed macaques by subcutaneous injection was rapidly disseminated from the inoculation site to the lymph nodes, adrenal glands, kidneys, heart, thyroid, liver, prostate gland, and seminal vesicles. Meanwhile, dissemination of dengue virus in pigtailed macaques inoculated intravenously was detected in lymph nodes, thymus, salivary glands, liver, and prostate gland. This study suggested that the above mentioned-tissue specimens are involved or affected by DEN-3 virus replication and the route of infection seemed to have influenced the virus dissemination.

Cloning and Expression of Serotype-2 Simian Betaretrovirus Reverse Transcriptase Gene Isolated from Indonesian Cynomolgus Monkey in Escherichia coli UUS SAEPULOH; DIAH ISKANDRIATI; FUNGKEY HOETAMA; SELA SEPTIMA MARIYA; DEDY DURYADI SOLIHIN; JOKO PAMUNGKAS; DONDIN SAJUTHI
Microbiology Indonesia Vol. 7 No. 2 (2013): June 2013
Publisher : Indonesian Society for microbiology

In this study, we isolated the simian betaretrovirus serotype-2 (SRV-2) reverse transcriptase (RT) gene from infected Indonesian cynomolgus monkey (Macaca fascicularis). The gene was then cloned in Escherichia coli expression system. The SRV-2 RT gene is located between nucleotides 3284-4925 in the polyprotein (Pol) region encodes 547 amino acids. Analysis of expression using SDS-PAGE and western blot techniques showed a specific band of 64.9 kDa, indicating SRV-2 RT recombinant enzyme. Purification of SRV-2 RT recombinant enzyme produced 312 μg mL-1 protein with 7.1 U μL-1 enzyme activities. Application of this recombinant enzyme in reverse transcription-PCR (RT-PCR) of β-globin and β-actin genes produced DNA fragments of 206 and 350 bp, indicating amplification of β-globin and β-actin genes, respectively. Therefore, the expressed SRV-2 RT enzyme was proven to be functional, although the activity was low.

Aqueous Leaf Extract of Senduduk (Melastoma malabathricum L.) Could Improve the Physicochemical Properties of Beef Sausage Dough Suharyanto Suharyanto; Henny Nuraini; Tuti Suryati; Irma Isnafia Arief; Dondin Sajuthi
Jurnal Ilmu dan Teknologi Hasil Ternak (JITEK) Vol. 15 No. 2 (2020)
Publisher : Faculty of Animal Science Universitas Brawijaya

Improving comminuted meat products characteristics using a natural agent, such as phytochemicals, in order to replace the use of nitrite, have become a need due to the health reason. The quality of the sausage is also affected by the initial characteristics of the dough. Therefore, this research was conducted to investigate the effect of aqueous leaf extract of senduduk (Melastoma malabathricum L.) on the physicochemical properties of beef sausage dough. Different four formulas as treatment were employed to form the dough: formula A was as a control consisted of beef meat, vegetable oil, skim milk powder, tapioca, salt, phosphate, and seasoning; formula B was control added with extract 0.55%; formula C was control added with sodium nitrite 0.0011%, and formula D was control added with extract 0.55% and sodium nitrite 0.0011%. All ingredients were blended to be the dough. The result of the study denoted that the extract (B and D) significantly decreased (P<0.05) pH, and aw value with no difference in water content among the dough. The total phenolic content of the dough containing extract (B and D) was markedly higher (P<0.05) than were others. It increased significantly on antioxidant capacity, scavenging activity, and reduced the thiobarbituric acid reactive substances (TBARS) value of the dough. There was also no nitrite residual detected in all dough. In conclusion, the extract could improve the physicochemical properties of beef sausage dough and replace the use of nitrite in the dough.

Association of CCL7 Promoter Polymorphism with Responsiveness to Allergen in Cynomolgus Macaque Model of Asthma Sela Septima Mariya; Fitriya Nur Annisa Dewi; Eric Hayes; Villiandra Villiandra; Yasmina Arditi Pramastri; Diah Iskandriati; Uus Saepuloh; Joko Pamungkas; I Nengah Budiarsa; Dondin Sajuthi
The Indonesian Biomedical Journal Vol 12, No 2 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i2.1093

BACKGROUND: C-C motif Ligand 7 (CCL7) has been reported to be associated with asthma severity in humans. Cynomolgus macaques (Macaca fascicularis; Mf) are often used as animal model of asthma but little is known about Mf genetic profile such as polymorphism. Our aim was to identify CCL7 polymorphism in Mf as a potential surrogate marker for identification of allergen responsiveness in the Mf model of asthma.METHODS: Real-time PCR was performed on archive of bronchoalveolar fluid samples previously collected from Mf that were exposed to allergen. Expression of CCL7 mRNA was evaluated, and sequencing technique was used to identify polymorphism in this gene.RESULTS: The results showed that CCL7 expression did not differ between Mf, despite a trend of lower expression in Mf that exhibited high response to allergen. By direct DNA sequencing of CCL7, 10 sequence variants were identified; three in promoter region (-460 G/A, -459 A/G, -456 -/A ), two in exon 1 (9 A/G, 65 G/C), four in intron 1 (135 T/C, 254 T/C, 420 T/C, 453 A/G), and one in intron 2 (1205 T/A).CONCLUSION: There was an association between Mf sensitivity to allergen with CCL7 promoter polymorphism at (-456 -/A). These results suggest that CCL7 may be a potential genetic marker to identify Mf sensitivity to allergen, which could be a useful tool to efficiently select for Mf model of asthma.KEYWORDS: asthma, CCL7, allergy, Ascaris suum, nonhuman primate

Model psikologi komparatif ketidaksetaraan jumlah pembagian sumber daya: Studi pada Macaca fascicularis Dwi Atmoko Agung Nugroho; Dondin Sajuthi; Sri Soepraptini Mansjoer; Entang Iskandar; Huda Shalahuddin Darusman
Jurnal Psikologi Sosial Vol 19 No 1 (2021): February
Publisher : Fakultas Psikologi Universitas Indonesia dan Ikatan Psikologi Sosial-HIMPSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.7454/jps.2021.03

This study aims to promote Macaca fascicularis as a comparative psychology model in finding the root and solution of resource inequity by exploring inhibitor effect on refusal behavior, acceptance, and aggression toward the feed distribution numbers in six pairs of female long-tailed macaques. We observed the frequency of refusal behavior, acceptance, and aggression toward the distribution of red grape with a ratio of: a) 1:1, 0:2, 1:3 without environmental inhibitors (with an opened-aclyric tray) and b) ratios 1:1 with an environmental inhibitor (with a transparent restriction box) in 60 trials per condition. The sample size was N = 10. Non-parametric statistical analysis of Wilcoxon Signed-Rank Test showed that 1:1 ratio with environmental inhibitor produces a lower response of refusal behavior compared to 1:3 without inhibitor. It produced a lower response of aggression compared to other ratios. It produced a greater acceptance compared to other ratios. These long-tailed macaques do not accept the equity conditions except with environmental inhibitors. Based on this fact, we conclude that long-tailed macaques are a good spontaneous model for the comparative psychology of inequity.

CCL2 and CCR2 Expression in Broncoalveolar Lavage Fluid of Cynomolgus Macaque Model Of Asthma Sela S Mariya; Fitriya N Dewi; Villiandra Villiandra; Yasmina A Pramastri; Diah Iskandriati; Eric Hayes; Joko Pamungkas; R.P Agus Lelana; Ligaya I Tumbelaka; Dondin Sajuthi
Jurnal Respirologi Indonesia Vol 38, No 2 (2018)
Publisher : Perhimpunan Dokter Paru Indonesia (PDPI)/The Indonesian Society of Respirology (ISR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36497/jri.v38i2.166

Background: Animal models are essential for the development and improvement of novel and effective methods for diagnostic and treatment of human diseases. Cynomolgus monkeys have been used as animal model in asthma studies wherein they exhibited different responses to allergen exposure in the airway; some were low responders while others were high responder. CCL2 is a potent chemotactic factor for monocytes and the gene expression was high in animal model of asthma. The aim of this study was to evaluate CCL2 and CCR2 expression between the low and high responders. Methods: Realtime PCR technique was used to evaluate CCL2 and CCR2 gene expression in bronchoalveolar lavage samples. The subject of this study was asthmatic cynomolgus monkeys, consisted of 4 low responders and 4 high responders. This study was held in Biotechnology Laboratory of Primate Research Center LPPM IPB on October 2016- January 2017. Results: CCL2 and CCR2 expression in low responders were higher than those in high responders at 24hours post airway challenge. Conclusion: CCL2 may potentially be developed as target for therapy or a genetic marker for asthma responsiveness in individuals. (J Respir Indo 2018; 38(2): 115-22)

Co-Authors . Permanawati Achmad - Taher Achmad Taher Achmad Taher Achmad, Taher Adi Winarto Agus Harsoyo Agus Harsoyo Agus Lelana Agus Wijaya Agus Wijaya Ahmad Kamal Nasution Alexander Nater Anita Esfandiari Annisa Dewi, Fitriya Nur Antonius Suwanto Anwar Wardy Warongan Arief Boediono Aryo Tedjo Bambang Purwantara Bayu Febram Prasetyo Bayu Rosadi Budiman Bela C Hanny Wijaya C. Hany Wijaya Cecep Kusmana CHAIRIN N MAROEF Chusnul Choliq Chusnul Choliq Chusnul Choliq Cucu K Sajuthi Dadang Makmun Daldiyono Daldiyono Daldiyono Daldiyono Dedi Rahmat Setiadi DEWI APRI ASTUTI Dewi, Fitriya Nur Annisa Diah Iskandriati Diah Iskandriati Diah Iskandriati DIAH ISKANDRIATI Diah Iskandriati DIAH ISKANDRIATI Diah Iskandriati Diah Iskandriati Douglas M. Bowden Dwi Atmoko Agung Nugroho DYAH PERWITASARI -FARAJALLAH Ekowati Handharyani Entang Iskandar Entang Iskandar Entang Iskandar Erdiansyah Rahmi Eric Hayes Eric Hayes ERIC HAYES ERLIN LISTIYANINGSIH Erni Sulistiawati ESTHER ARIFIN Fauzi Abdurrahman Munggaran Fransiska R Zakaria FUNGKEY HOETAMA Gede Eko Darmono Gregory K. Wilkerson Hadi S Alikodra Hajrawati Hajrawati Harrold Halilintar MR Subu Taopan Henny Nuraini HERA MAHESHWARI Huda S Darusman Huda Shalahuddin Darusman Huda Shalahudin Darusman I Gusti Agung Arta Putra I Gusti Ngurah Sudisma I Ketut Sutama I Nengah Budiarsa I NENGAH BUDIARSA I Nengah Budiarsa I wayan Teguh Wibawan IETJE WIENTARSIH Iis Afriantini Iman Rusmana Iman Supriatna IMELDA WINOTO Indra Bachtiar Indra Bachtiar Irawan Jusuf Irma H Suparto Irma H Suparto Irma H Suparto Irma Herawati Suparto Irma Isnafia Arief Irma Suparto J. Mark Cline Jansen Manansang Jansen Manansang Jayusman Arsiyanti Joesoef JONATHAN STURGIS Kanti Rahmi Fauziyah Kartika Sari KATHARINA JESSICA KEVIN RANDALL PORTER Koeswinarning Sigit Latifah K. Darusman Latifah K. Darusman Leni Maylina Lina Elfita Lina Elfita Loe, Fhady Risckhy Luluk Lailatul Hasanah LUTHFIRALDA SJAHFIRDI Luthfiralda Sjahfirji M Agus Setiadi MARYATI SURYA Maxs Urias Ebenheizer Sanam Michael Krützen MOSES AFFANDI Mozes R. Tolihere Muchidin Noordin MUHAMMAD AGIL Nanik Hidayatik Nur Hidayati Nurhidayat . Nurul Oktaviani Oktaviani, Nurul Oriza Savitri Ariantie Paini Sri Widyati Pamungkas, Joko PATRICK J BLAIR Peni Harjosworo PENI SUPRAPTI HARDJOSWORO PERMANAWATI PERMANAWATI Permanawati Permanawati Permanawati Permanawati PERMANAWATI, PERMANAWATI Pijoh, Deyv Praira, Willy PUDJI ASTUTI Pudji Astuti Pudji Astuti Puji Rianti Puspita Sari Putri, Zulfitra Utami R. Iis Arifiantini R.P Agus Lelana RATNA TAN Retno Wulansari Reviany Widjajakusuma Reviany Widjajakusuma Ria Oktarina RICHARD F GRANT Rini Widayanti Sajuthi, Cucu K Sela S Mariya Sela S. Mariya SELA SEPTIMA MARIYA Sela Septima Mariya Septi Iriani Setyaningsih Rambu Liwa Setyaningsih Rambu Liwa Setyo Widi Nugroho Setyo Widodo Silmi Mariya SILMI MARIYA Silmi Mariya SILMI MARIYA Silmi Mariya Silmi Mariya Silvia Triwidyaningtyas Sony Hartono Wijaya Sri Rahmatul Laila Sri Soepraptini Mansjoer Sri Supraptini Mansjoer Srihadi Agungpriyono Subu Taopan, Harrold Halilintar MR Suharyanto Suharyanto Suharyanto Suharyanto Sulistiyani Sulistiyani . SULISTIYANI SULISTIYANI Sulistiyani Sulistiyani SULISTIYANI SULISTIYANI Sulistiyani, Sulistiyani Suparto, Irma Sus Derthi Widhyai Sus Derthi Widhyari SUSAN SOKA SUSANA WIDJAJA Suzy Tomongo Taher, Achmad TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Teguh Ari Prabowo Teguh Budipitojo Tejasari . Thomas Mata Hine Tiara Widyaputri Tri Wahyu Pangestiningsih Trini i Susmiat Trini Susmiati Tuti Suryati Tuty L Yusuf Tuty Laswardi Yusuf Tuty Laswardy Yusuf Unang Supratman Uus Saefullah UUS SAEPULOH UUS SAEPULOH Uus Saepuloh Uus Saepuloh Uus Saepuloh Uus Saepuloh Vetnizah Juniantito Villiandra Villiandra Villiandra Villiandra Villiandra Villiandra Vincentia Trisna Yoelinda Willy Praira Yanne Yanse Rumlaklak Yanti . Yanti Yanti Yasmina A Pramastri Yasmina A. Paramastri Yasmina Arditi Pramastri YASMINA PARAMASTRI Yoga Yuniadi Yohana Tri Hastuti Yohana Tri Hastuti Yohana Tri Hastuti Yuli Purwandari Kristiangingrum Yundari, Yundari Zulfitra Utami Putri Zulfitra Utami Putri Zuraida Zuraida Zuraida

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