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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya Majalah Kedokteran Bandung The Journal of Experimental Life Sciences (JELS) e-Journal Pustaka Kesehatan Research Journal of Life Science Journal of Agromedicine and Medical Sciences Mutiara Medika: Jurnal Kedokteran dan Kesehatan UNEJ e-Proceeding Sari Pediatri Universa Medicina Majalah Kesehatan FKUB Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Indian Journal of Forensic Medicine & Toxicology CRJIM (Clinical and Research Journal in Internal Medicine) Lux Mensana: Journal of Scientific Health Jurnal Penelitian IJRETINA - International Journal of Retina
Loeki Enggar Fitri
Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Public Health Other

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Endoglin Expression and the Level of Tgf-Î’ Are Increased In the Placental Tissue and Correlated with Low Fetal Weight in Malaria Infected Mice sasmito, sujarot dwi; Ulfiati, Adilah; Wardana, Ardhian; Nugraheni, Fitriana; Pradiptasari, Nur Fahma; Zulaifa, Zakiyah; Norahmawati, Eviana; Sardjono, Teguh Wahju; Fitri, Loeki Enggar
Journal of Tropical Life Science Vol 5, No 1 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.5.1.%x

Abstract

Malaria infection during pregnancy can cause accumulation of infected-red blood cells in placental intervillous space and induces placental tissue inflammation and hypoxia. This condition triggers endoglin expression, and release of soluble endoglin which can interfere TGF-β binding to its receptor. The aim of this study was to investigate the correlation between placental endoglin expression and TGF-β level with low fetal weight (LFW) in malaria-infected mice. Nine pregnant mice infected with Plasmodium berghei on the day 9th post mating (malaria infected group) and eight normal pregnant mice (non-infected group) were used in this study. The mice were sacrificed on the day 18th post mating, and all fetal body weights were measured by analytical scale. Enzyme Link Immunosorbent Assay (ELISA) was done to determine the level of placental TGF-β while immunohistochemical staining was performed to examine endoglin expression in placental tissue. The mean of fetal body weights of malaria infected group was significantly lower than non-infected group (p=0,002), while the expression of placental endoglin in malaria infected group was significantly higher than non-infected group (p=0.003). The level of placental TGF-β in malaria infected group was also higher than non-infected group but the difference was not significant (p=0.064). Pearson correlation test showed that there were significant negative correlations between fetal body weights with the level of placental TGF-β (p=0.017, r=-0.568) and the expression of placental endoglin (p=0.002, r=-0.694). Malaria infection in pregnant mice will increase both TGF- β and endoglin in placenta tissue and correlate with low fetal weight.   
Toll Like Receptor 4 (Tlr4) And p65 Nuclear Factor Kappa B (Nf-Kb) Expression In Monocyte Cell Of Children With Steroid Resistant Nephrotic Syndrome Wahyono, Tikto; Subandiyah, Krisni; Fitri, Loeki Enggar; Endharti, Agustina Tri
Journal of Tropical Life Science Vol 4, No 2 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Excess Fibrin Deposits Decrease Fetal Weight of Pregnant Mice Infected by Plasmodium berghei Andari, Desy; Rahma, Zainabur; yuliyanik, Yuliyanik; Sasmito, Sujarot Dwi; Mintaroem, Karyono; Fitri, Loeki Enggar
Journal of Tropical Life Science Vol 4, No 2 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Low birth weight is commonly attributed to malaria in pregnancy, but the cellular and molecular mechanisms that underlie this poor birth outcome are incompletely understood. A universally described histopathological feature of placental malaria is excessive deposition of fibrin, the end-product of the coagulation cascade. This study was conducted to compare fibrin deposit in pregnant mice that infected by Plasmodium berghei (treatment group) to the normal pregnant mice (control group) and its association with fetal weight. This research is in vivo experimental laboratory study that used 18 pregnant Balb/c mice which divided to the control the group (8 mice) and treatment group (9 mice infected by P.berghei). Placentas were staining with Haematoxylin-Eosin (HE) for fibrin deposits examination whereas fetal weight was performed with Mettler analytical balance AE 50. Fetal weight of the treatment group was lower than those of the control group (t test, p=0,002). Fibrin deposits were increased in the treatment group (t test, p=0,005) and influenced weight of fetuses (Spearman r=-0,586, p=0,014). Weights of fetuses are interfered by fibrin deposits during malaria infection.
Intraperitoneal Injection of High Tumor Necrosis Factor (TNF-α) Serum Increase Soluble Fms-like Tyrosine Kinase-1 (sFlt-1) and Blood Pressure of Pregnant Mice Wicaksono, Bramantyo Aji; Baktiyani, Siti Candra Windu; Fitri, Loeki Enggar
Journal of Tropical Life Science Vol 5, No 2 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.02.01

Abstract

Preeclampsia has major symptoms of hypertension and proteinuria and is a cause of significant maternal and infant mortality in the world. The slow development of preeclampsia research possibility caused by the difficulty in acquiring animal preeclampsia. Many existing animal model have been developed, but most of them are expensive to do. The purpose of this study was to determine the effects of intraperitoneal injection of pregnant patients serum with high TNF-α levels toward sFlt-1 serum concentration and blood pressure of pregnant mice. Pregnant patients serum with high TNF-α levels (>20 pg/mL) was injected intraperitoneally to pregnant mice at gestational age 13 and 14 days. At 18 days of gestation, the blood pressure was measured, then the mice were dissected and the serum was taken to measure serum sFlt-1 concentration using ELISA kit(Bioassay Technology Laboratory, E0611Mo). The results showed there was a significant increase in blood pressure (p = 0.000) and the sFlt-1 levels (p = 0.002) of pregnant mice group that injected by pregnant patient serum with high TNF-α levels compare to control group. From these data it can be concluded that intraperitoneal injection of pregnant patients serum with high TNF-α levels to pregnant mice can increase blood pressure and sFlt-1 serum concentration of mice.
Optimation of Combination of N-Hexane Solution and Ethyle Acetate on Secondary Metabolite Compounds Profile of Streptomyces hygroscopicus Ariel, Dio Giovanni; Winarsih, Sri; Putri, Fitria Febriliani; Erwan, Nabila Erina; Putri, Ajeng Maharani; Cahyono, Alfian Wika; Mardhiyyah, Kana; Fitri, Loeki Enggar; Nugraha, Rivo Yudhinata Brian
Jurnal Kedokteran Brawijaya Vol 31, No 3 (2021)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2021.031.03.11

Abstract

Streptomyces hygroscopicus (S.hygroscopicus) is a Gram-positive soil bacterium that can produce secondary metabolites from fermentation that have a therapeutic effect. The fermented S. hygrocospicus metabolites that are still in the form of crude extracts are difficult to develop as drug preparations because the active compounds are not yet known, so it will be challenging to determine the dosage of drugs that have a therapeutic effect. Therefore, it is necessary to carry out exploratory research to narrow down the secondary metabolite profile from the fermentation of S. hygroscopicus, using extraction and fractionation methods, which are then identified by Thin-Layer Chromatography (TLC) using a combination of solvents. This study used the extraction method with a separating funnel. The fractionation was carried out using the BUCHI (Sepacore®) Flash Chromatography and Reveleris® PREP Purification System column chromatography gradually using ethyl acetate and n-hexana.  47 and 60 of the fractionation results were taken as samples, that further were profiled using TLC and given the appearance of 10% KOH stains and p-Anisaldehyde - sulfuric acid, so that various classes of compounds with different Rf values were obtained, namely Monoterpenes, Triterpenes, Steroids, Saponins, Coumarin, Scopoletin, and Alkaloids.
Uji Knockdown Effect Ekstrak Bunga Syzygium aromaticum L. terhadap Nyamuk Culex Sp. Dewasa Nopitasari, Hayu Sukowati; Fitri, Loeki Enggar; Nurdiana, Nurdiana
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 14, No 1 (2014)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v14i1.2473

Abstract

Nyamuk Culex sp. merupakan vektor penyakit Filariasis, Japanese encephalitis dan demam chikungunya. Insektisida dipilih untuk mengontrol populasi Culex sp. namun, penggunaan insektisida menimbulkan resistensi nyamuk dan efek toksik pada manusia. Oleh karena itu, diperlukan adanya insektisida yang lebih aman bagi lingkungan. Salah satunya adalah dengan menggunakan ekstrak bunga cengkeh (Syzygium aromaticum L.) yang mengandung carvone, terpinen-4-ol, fenchone, eugenol dan quercetine yang berpotensi sebagai insektisida. Tujuan dari penelitian ini adalah untuk membuktikan bahwa ekstrak bunga S. aromaticum L. memiliki knockdown effect terhadap nyamuk Culex sp. Penelitian ini merupakan penelitian eksperimental laboratoris dengan rancangan true experimental-post test only con­trol group design. Sampel yang digunakan adalah 25 ekor nyamuk Culex sp. pada setiap perlakuan. Konsentrasi ekstrak bunga S. aromaticum L. yang digunakan adalah 1,25%, 2,5%, 5% dan sebagai kontrol negatif digunakan larutan aseton 1% serta malathion 0,28% sebagai kontrol positif. Dari uji Kruskal-Wallis diketahui bahwa ekstrak bunga S. aromaticum L. pada menit ke-5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55 dan 60 memberikan perbedaan yang signifikan diantara konsentrasi 1,25%, 2,5%, 5% dan malathion 0,28% (p 0,05). Uji korelasi Spearman membuktikan adanya hubungan yang kuat antara knockdown effect dengan besarnya konsentrasi ekstrak bunga S. aromaticum L. (p=0,000 dan r=0,907). Disimpulkan bahwa ekstrak bunga S. aromaticum L. memiliki knockdown effect terhadap nyamuk Culex sp. dewasa.Culex is vector for Filariasis, Japanese encephalitis and Chikungunya fever. Insecticide is chosen to control Culex sp. population. However, the usage of chemical insecticides make insecticide resistant and toxic effect. Therefore, safer alternative insecticide for environment is needed. One of them is by using clove bud extract. Previous studies has proved that clove bud extract (Syzygium aromaticum L.) has containing carvone, terpinen-4-ol, fenchone, eugenol and quercetine a potency to be insecticide on Culex sp. The purpose of this experiment is to prove whether S. aromaticum L. bud extract has knokdown effect on Culex sp. This research was a laboratorial experimental research using true experimental post test only control group design. The samples were 25 Culex sp. in every group which treated by S. aromaticum L. bud extract (1,25%, 2,5%, 5%) and aceton preparation (1%) as a negative control, malathion preparation (0,28%) as a positive control. Kruskal-Wallis test showed that S. aromaticum L. bud extract in 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55 and 60 minute gave significant difference among concentration 1,25%, 2,5% and 5% (p 0,05). Spearman corelation test prove that there was a significant relationship between knockdown effect produced and the concentration of the S. aromaticum L. bud extract (p=0,000 and r=0,907). It can be concluded that S. aromaticum L. bud extract has knockdown effect against adult Culex sp.
The Effect of Artesunate and Brotowali (Tinospora Crispa) Combination on Histopathological, and Expression of Nuclear Factor Kappa B (NF-kβ) in Renal Tubules of Mice Infected With Plasmodium Berghei Merici, Angela; Fitri, Loeki Enggar; Budiarti, Niniek
Clinical and Research Journal in Internal Medicine Vol 1, No 1 (2020): New: The First Volume of Clinical and Research Journal in Internal Medicine
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.crjim.2020.001.01.5

Abstract

Brotowali (BR) extract (Tinospora crispa) can be used as an antimalarial. Aim: to determine the effect of BR extract in histopathological and expression of NFκB in mice tubules infected by Plasmodium berghei treated by artesunate (AR). Method: we used 42 C57BL / 6J strain mice as experimental animals, which were randomly divided into 7 groups : negative control (NC), positive control (PC), treatment group consist of AR 32 mg/kb (group 1); BR 70 mg/kg (group 2), combination of AR+BR 50 mg/kg (group 3), AR+BR 60 mg/kg (group 4), and AR+BR 70 mg/kg (group 5).  Histological examination (hematoxylin-eosin (HE) staining) and expression of NFKB (immunohistochemical staining) in the kidneys were performed on 7th and 14th. Result: compared to PC group, BR with doses of 70 mg until 14th day, improved the degree of tubular necrosis, interstitial fibrosis, tubular degeneration, and inflammatory cell infiltration (p <0.001) but did not reach NC group (p <0.05). The combination of AR+BR until the 14th  day with dose of 50, 60, 70 mg all of dose improves significantly in-term of degree of tubular cell necrosis and inflammatory cell infiltration. The degree of interstitial fibrosis on 14th day only improved in group 4 and 5 (p<0.001 and p=0.003). The level of NF-kB expression on day 7 and day 14 was reduced in group 2, group 4, and group 5 compared to PC group. There was positive correlation on 7th and 14th between NF-kβ expression and tubular degeneration, tubular cell necrosis, inflammatory cell infiltration, and interstitial fibrosis. Conclusion: the combination of AR+BR extract can improve histopathological features and reduce NF-kβ expression in mice tubules infected by Plasmodium berghei with an optimal dose was 60 mg/day for 7-14 days or 70 mg for 7 days.
Analysis of Nurse Personal Factors of Triage Decision-Making in Emergency Installation at University of Muhammadiyah Malang Hospital Ali Rahmanto; Loeki Enggar Fitri; Ika Setyo Rini
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.16837

Abstract

The high frequency of patient visits to the ER will disrupt the service process at the Emergency Department(IGD). One solution is the application of a triage system, which is the process of sorting patients accordingto their level of gravity. The accuracy of triage decisions has a significant impact on patient outcomes sothat in this case nurses in the ER must have knowledge, understanding, and skills about triage. Analyzepersonal factors that influence emergency nurse decision-making. This study aimed to identify personalfactors influencing triage decision-making among emergency nurses. Respondents were 30 male and femalenurses who worked and were active up to now in the IGD Hospital of the University of MuhammadiyahMalang. Data on work experience, education, and training were taken from data on the characteristics ofrespondents. The knowledge questionnaire came from the Emergency nurses association/ENA while theskills questionnaire used the Triage Skill Questionnaire/TSQ. The Spearman correlation test shows that thereis a significant relationship between skills and education with triage decision making (p=0.000; r=0.626 andp=0.039; r=0.378). Meanwhile, the variables of knowledge, work experience, and training do not correlatewith triage decision making (p> 0.05) except that GELS training shows a significant relationship betweenGELS training and triage decision making (p=0.016; r=0.437). The results of linear regression analysis,skills, and education are factors that influence decision-making in conducting triage in the ER (45,4%).Skills and education are the factors that most influence triage decision-making.
Vitamin D3 Adjuvant Treatment Stimulate Interleukin-10 Expression in Children with Nephrotic Syndrome Without Affecting to Clinical Outcome and Glucocorticoid Receptor Expression Asariati, Husnul; Subandiyah, Krisni; Fitri, Loeki Enggar
Journal of Tropical Life Science Vol 4, No 3 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Prominently Increased of Mannose Binding Lectin (MBL) and Myeloperoxidase (MPO) Levels in Severe Valve Regurgitation and Heart Failure of Rheumatic Heart Disease Putri, Rachmania; Suwarniaty, Renny; Fitri, Loeki Enggar; Nugroho, Susanto; Rahman, Mohammad Saifur
Journal of Tropical Life Science Vol 7, No 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.04

Abstract

Rheumatic heart disease (RHD) is mediated by an abnormal immunological response following a Streptococcus pyogenes infection that induces a disturbance of oxidants and antioxidants balances. Mannose-binding lectin (MBL) binds to N-acetylglucosamine, a molecule present on the Streptococcus cell wall and human heart valves. There is a disturbance of oxidant and antioxidant balance in rheumatic disease. Myeloperoxidase (MPO) is a marker of oxidative stress and inflammation. This study was aimed to determine the correlation of MBL and MPO levels and severity of valvular regurgitation and heart failure (HF) in RHD patients. A case-control study was conduct using human peripheral blood samples from 32 children aged 6 to 14 years old. The subjects were divided into two groups: 16 RHD patients included in the case group and 16 healthy children as a control group. The level of MBL and MPO was investigated using ELISA method. There were significant differences on MBL and MPO level between patient and control group. The level of MBL and MPO were significantly increased in RHD group, especially on severe valvular regurgitation. There was a strong correlation between MBL and MPO levels and the severity of valvular regurgitation (r = 0.94 and r = 0.88). The least significant diff-erence (LSD) analysis showed that significant difference occurs in the severe heart failure group. Our research revealed that the MBL and MPO levels in pediatric RHD patients were significantly higher than in healthy children. The MBL and MPO levels were significantly correlated with the severity of valvular regurgitation and heart failure.
Co-Authors A.A. Ketut Agung Cahyawan W AA Sudharmawan, AA Adilah Ulfiati Adilah Ulfiati, Adilah Agustin Iskandar Agustina Tri Endharti Agustina Tri Endharti Ahmad Fauziansyah Zian Ajeng Maharani Putri Alfian Wika Cahyono Ali Rahmanto Anak Agung Gede Sugianthara Andi Arahmadani Arasy Anik Widijanti Arasy, Andi Arahmadani Ardhian Wardana Ardhian Wardana, Ardhian Ariel, Dio Giovanni Arif Setyawan, Sang Aji Arifin, Mochammad Samsul Astutik Pudjirahaju Bagus Hermansyah BR. Sijabat, Melda Fio Flora Bramantyo Aji Wicaksono Bramantyo Aji Wicaksono, Bramantyo Aji Brigitta Ida RV Corebima Burhan, Niniek Cahyono, Alfian Wika Chairul A. Nidom Chairul A. Nidom Dalhar, Mochamad Damayanti, Ria Dara Dasawulansari Syamsuri Desy Andari Dewi Indiastari Didi Candradikusuma Didi Candradikusuma Dio Giovanni Ariel Dorta Simamora Edi Widjajanto Elviliana, Elviliana Erma Sulistyaningsih Erwan, Nabila Erina Eviana Norahmawati Firdiani Nur Afifah, Firdiani Nur Fitria Febriliani Putri Fitriana Nugraheni Fitriana Nugraheni, Fitriana Ghofar, Hervin Febrina Handono Kalim Hayu Sukowati Nopitasari Heri Kristianto Heri Suroso, Heri Herpan Syafii Harahap Hervin Febrina Ghofar Hidayat Sujuti Husnul Asariati Husnul Asariati I Ketut Gede Muliartha Ihwan Ihwan Ika Setyo Rini Irene Ratridewi Isnadiyah Juhdi Joko Agus Gunawan Josef Sem Berth Tuda Juhdi, Isnadiyah Kana Mardhiyyah Kartika Senjarini Karyono Mintaroem Karyono Mintaroem Karyono Mintaroem Khadafi Indrawan Khairiyadi Khairiyadi Krisni Subandiyah Krisni Subandiyah Krisni Subandiyah Krsni Subandiyah Kusworini Handono Laksmi Karunia Tanuwijaya Laksmi Karunia Tanuwijaya Lilik Maslachah Lilik Zuhriyah M. Fahrul Arifin Mardhiyyah, Kana Mario B. Nara Melda Fio Flora BR. Sijabat Merici, Angela Mochammad Dalhar Moh Mirza Nuryady Moh Mirza Nuryady, Moh Mirza Mohammad Saifur Rahman Mulyohadi Ali Muti'ah, Roihatul Nabila Erina Erwan Nafi’ah, Riris Waladatun Namira, Anggi Alya Nashi Widodo Natalia Erica Jahja Nicole Berens-Riha Nicole Berens-Riha, Nicole Niniek Budiarti B Niniek Burhan Noer Aini Nopitasari, Hayu Sukowati Nugraha, Rivo Yudhinata Brian Nur Fahma Pradiptasari Nur Fahma Pradiptasari, Nur Fahma Nur Hidayat Nur Permatasari Nurdiana Nurdiana Nurdiana Nurdiana Nurtyas, Yunika pertiwi, resti anggun Poedji Hastutiek Pratama, Andhika Putra Agus putra, prasaundra triantoni Putri, Ajeng Maharani Putri, Fitria Febriliani Putri, Rachmania Rachmania Putri Rahma, Zainabur Rahmad Rahmad Rahman, Mohammad Saifur Renny Suwarniaty Renny Suwarniaty, Renny Rifa'i, Muhaimin Rivo Yudhinata Brian Nugraha Rizky Amalia Rohma, Novita Ainur Sabrina Sunyoto, Nimas Mayang Saiful Akhyar Lubis Sakunda Anggarini, Sakunda Samsul Arifin Siti Candra Windu Baktiyani Siti Candra Windu Baktiyani Soebaktiningsih Soebaktiningsih Soemarko Soemarko Sofia, Ovi Sri Hayati, Yati Sri Murwani Sri Poeranto, Sri Sri Suhartini Sri Winarsih Subekti, Indah Fitriana Sudjari Sudjari Sugeng Setyo Utomo Sugeng Setyo Utomo, Sugeng Setyo Sujarot Dwi Sasmito sujarot dwi sasmito, sujarot dwi Sumarno . Susanto Nugroho Susanto Nugroho Sutiman B. Sumitro Swastomo, Rahadi Syarkiah Syarkiah Teguh Wahju Sardjono Teguh Wahju Sardjono Teguh Wahju Sardjono Thomas Loescher Thomas Loescher, Thomas Tikto Wahyono Tinny Endang Endang H Tinny Endang Hernowati Tinny Endang Hernowati Tuda, Josef Sem Berth Umar Zein Verry Asfirizal Vonny Mariany Deckert Wahyono, Tikto Wongso Suhendro Yoes Prijatna Dachlan Yuanita Mulyastuti Yuliyanik yuliyanik Yunika Nurtyas Yunita Armiyanti Zainabur Rahma Zakiyah Zulaifa Zakiyah Zulaifa, Zakiyah