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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya Majalah Kedokteran Bandung The Journal of Experimental Life Sciences (JELS) e-Journal Pustaka Kesehatan Research Journal of Life Science Journal of Agromedicine and Medical Sciences Mutiara Medika: Jurnal Kedokteran dan Kesehatan UNEJ e-Proceeding Sari Pediatri Universa Medicina Majalah Kesehatan FKUB Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Indian Journal of Forensic Medicine & Toxicology CRJIM (Clinical and Research Journal in Internal Medicine) Lux Mensana: Journal of Scientific Health Jurnal Penelitian IJRETINA - International Journal of Retina
Loeki Enggar Fitri
Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Public Health Other

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Difference of Vitamin D and Interleukin-6 Levels in Children with Steroid- Resistant, Steroid-Sensitive and Idiopathic Nephrotic Syndrome Krisni Subandiyah; Hervin Febrina Ghofar; Loeki Enggar Fitri
Journal of Tropical Life Science Vol. 9 No. 2 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.02.07

Abstract

Idiopathic nephrotic syndrome (INS) is the most prevalent autoimmune glomerular disease in children and its pathogenesis is correlated with high level of interleukin 6 (IL-6) and low level of vitamin D. This study was aimed to investigate the difference of vitamin D and IL-6 level in steroid resistant nephrotic syndrome (SRNS), steroid sensitive (SSNS), and idiopathic nephrotic syndrome (INS). This research was designed as cross sectional involving 45 subjects which then divided into 3 groups as follows: SRNS, SSNS, and INS. A level of serum 25 (OH)D was measured by Enzyme-linked Immuno Assay Method then categorized as sufficiency, insufficiency, and deficiency. Level of IL-6 serum was measured by ELISA method. Results showed that IL-6 level was significantly different among three groups, in which SRNS had the highest value (ANOVA, p < 0.05). Further analysis demonstrated that IL-6 level correlated with steroid resistance (Spearman correlation test, p = 0.000, r = 0.692). Vitamin D status was significantly different among three groups (Chi square, p = 0.03) and associated with steroid resistance (Spearman correlation test, p = 0.000, r = 0.568). Moreover, IL-6 level associated with 25 (OH) D level in SRNS group (Pearson correlation test, p = 0.020, r = 0.591) but not in the SSNS and INS group. We conclude that IL-6 levels were significantly higher in SRNS group as compared to other groups. Otherwise, vitamin D status were significantly lower in SRNS compared with other groups. An IL-6 level was negatively correlated with vitamin D status in patients with NS, specifically in SRNS group.
Ovitrap Index and Transovarial Transmission Rate of Dengue Virus of Male and Female Aedes aegypti Mosquitoes in Makassar, South Sulawesi, Indonesia Isnadiyah Juhdi; Loeki Enggar Fitri; Lilik Zuhriyah; Andi Arahmadani Arasy
Journal of Tropical Life Science Vol. 9 No. 1 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.01.13

Abstract

The incidence rate (IR) of dengue hemorrhagic fever (DHF) in Indonesia has increased in recent years and still causes a health problem. Makassar, as one of the big cities in Indonesia, is also experiencing of DHF cases. According to the Health Office of Makassar City, DHF cases during 2014 – 2016 has increased from 139 to 250 cases. The defense mechanism of dengue virus in transovarial transmission from female mosquitoes to eggs is estimated to be one of the factors that increase the rate of DHF. In addition, the circulation of the Aedes aegypti mosquitoes can also affect the increase of the rate. This study aims are to identify ovitrap index and to find out how much the vertical transmission of dengue virus by measuring the minimum infection rate (MIR) and the distribution of four dengue virus serotype in Ae. aegypti mosquitoes in the city of Makassar, South Sulawesi, as well as their association with the incidence rate (IR) in the area. This observational research uses ecological study with cross-sectional approach. The samples of the study are male and female mosquitoes from Ae.aegypti eggs obtained from the installation of ovitrap  in 30 urban villages in Makassar. Dengue virus identification was performed using a reverse transcriptase polymerase chain reaction (RT-PCR). These results showed that the ovitrap index (IO) was in the range of 0 - 66.7%. The average value of the ovitrap index outside the house (44.44%) was greater than inside house (37.22%). The results of identification of dengue virus using RT-PCR showed negative results as indicated by the non-formation of a band on 75 pools male and 78 pools female Ae. aegypti mosquitoes after electrophoresis. The results of this study indicated that the Ae. aegypti mosquito in Makassar City showed a tendency to lay eggs outside the house due to the habit of the people who put rain water shelters outside the house which could become breeding place for Ae. aegypti mosquitoes. This study also showed that there was no transovarial transmission of DENV found in male and female Ae. aegypti mosquitoes in Makassar City during November 2017 until January 2018.
Optimation of Combination of N-Hexane Solution and Ethyle Acetate on Secondary Metabolite Compounds Profile of Streptomyces hygroscopicus Dio Giovanni Ariel; Sri Winarsih; Fitria Febriliani Putri; Nabila Erina Erwan; Ajeng Maharani Putri; Alfian Wika Cahyono; Kana Mardhiyyah; Loeki Enggar Fitri; Rivo Yudhinata Brian Nugraha
Jurnal Kedokteran Brawijaya Vol. 31 No. 3 (2021)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2021.031.03.11

Abstract

Streptomyces hygroscopicus (S.hygroscopicus) is a Gram-positive soil bacterium that can produce secondary metabolites from fermentation that have a therapeutic effect. The fermented S. hygrocospicus metabolites that are still in the form of crude extracts are difficult to develop as drug preparations because the active compounds are not yet known, so it will be challenging to determine the dosage of drugs that have a therapeutic effect. Therefore, it is necessary to carry out exploratory research to narrow down the secondary metabolite profile from the fermentation of S. hygroscopicus, using extraction and fractionation methods, which are then identified by Thin-Layer Chromatography (TLC) using a combination of solvents. This study used the extraction method with a separating funnel. The fractionation was carried out using the BUCHI (Sepacore®) Flash Chromatography and Reveleris® PREP Purification System column chromatography gradually using ethyl acetate and n-hexana.  47 and 60 of the fractionation results were taken as samples, that further were profiled using TLC and given the appearance of 10% KOH stains and p-Anisaldehyde - sulfuric acid, so that various classes of compounds with different Rf values were obtained, namely Monoterpenes, Triterpenes, Steroids, Saponins, Coumarin, Scopoletin, and Alkaloids.
PENGARUH TELEREHABILITASI SELF CARE OREM TERHADAP MOBILISASI PASIEN PASCA STROKE: The Effect of Orem’s Self Care Telerehabilitation on Mobilization of Post Stroke Patients Mohammad Fahrul Arifin; Heri Kristianto; Loeki Enggar Fitri; Ahmad Fauziansyah
Jurnal Penelitian Sekolah Tinggi Ilmu Kesehatan Nahdlatul Ulama Tuban Vol. 6 No. 1 (2024): JURNAL PENELITIAN
Publisher : Institut Ilmu Kesehatan Nahdlatul Ulama Tuban

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47710/jp.v6i1.358

Abstract

Post-stroke patients need to receive telerehabilitation to increase mobility, telerehabilitation approaches Orem's Self Care Theory to increase mobility so that recurrent strokes will be greatly minimized. The research method for this research is Quasi Experimental with a pre-post test design approach and purposive sampling technique. The population is post-stroke patients in the Neurology Polyclinic and Orchid Room at RSUD Dr. R Koesma Tuban numbered 50 patients. 25 people in the control group were given education and 25 people in the intervention group were given a rehabilitation video. The chi square test and normality test were carried out and data analysis used the Mann Whitney test. Mobilization during the pre test with the average score in the control group was 23.72 and the intervention group was 27.28. The Std value. Deviation 10.016 with p value = 0.381. Mobilization during the post test with the average score in the control group was 31.8 and the intervention group was 19.2. The Std value. Deviation 16.427 with p value = 0.002. Based on the results of this study, it was found that there was a significant increase in mobilization scores after being given telerehabilitation in the intervention group.   Keywords:  Mobilization; Orem's Self Care; Telerehabilitation
THE ROLE OF COMMUNITIES LIVING IN WATERSHEDS TOWARDS FLOOD DISASTER MANAGEMENT : SYSTEMATIC REVIEW Suroso, Heri; Enggar Fitri, Loeki; Sri Hayati, Yati
Lux Mensana: Journal of Scientific Health Vol. 1 Issue 4 (2022)
Publisher : jfpublisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56943/jsh.v1i4.134

Abstract

Introduction. Disaster is a series of events that can threaten and disrupt human life, caused by natural and non-natural factors. A flood disaster is a natural disaster that can occur due to extreme storms in the upper watersheds, climate change, and geomorphological conditions of soil slopes, erosion, and sediment material. Flood disasters will have an impact on the social situation, people's economy, health status and death. Method. A systematic review was conducted through several stages, namely making questions, identifying, eligibility, selecting article inclusions, screening, and appraisal. The selection process was listed in the Systematic review framework and 10 articles are obtained. Later, articles were summarized and rated according to The Joanna Briggs Institute (JBI) in each article. Result&Analysis. The data shows that the results vary according to the system, and the conditions in which the study was conducted. Increasing the role of the community in flood disaster management efforts is done by increasing community knowledge, skills and experience, related to an efficient disaster management system, with several methods, namely by giving questionnaires and conducting interviews with the community or family, special training and simulations. Discussion. To increase the awareness and role of the community in managing flood disasters, several methods are applied. The simulation method is an educational effort that can be used to improve people's knowledge, skills, and experience in conducting flood management activities.
PENGARUH TELEREHABILITASI SELF CARE OREM TERHADAP MOBILISASI PASIEN PASCA STROKE: The Effect of Orem’s Self Care Telerehabilitation on Mobilization of Post Stroke Patients Mohammad Fahrul Arifin; Heri Kristianto; Loeki Enggar Fitri; Ahmad Fauziansyah; Arifin, Mohammad Fahrul Arifin
Jurnal Penelitian Vol 6 No 1 (2024): JURNAL PENELITIAN
Publisher : Institut Ilmu Kesehatan Nahdlatul Ulama Tuban

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47710/jp.v6i1.358

Abstract

Post-stroke patients need to receive telerehabilitation to increase mobility, telerehabilitation approaches Orem's Self Care Theory to increase mobility so that recurrent strokes will be greatly minimized. The research method for this research is Quasi Experimental with a pre-post test design approach and purposive sampling technique. The population is post-stroke patients in the Neurology Polyclinic and Orchid Room at RSUD Dr. R Koesma Tuban numbered 50 patients. 25 people in the control group were given education and 25 people in the intervention group were given a rehabilitation video. The chi square test and normality test were carried out and data analysis used the Mann Whitney test. Mobilization during the pre test with the average score in the control group was 23.72 and the intervention group was 27.28. The Std value. Deviation 10.016 with p value = 0.381. Mobilization during the post test with the average score in the control group was 31.8 and the intervention group was 19.2. The Std value. Deviation 16.427 with p value = 0.002. Based on the results of this study, it was found that there was a significant increase in mobilization scores after being given telerehabilitation in the intervention group.   Keywords:  Mobilization; Orem's Self Care; Telerehabilitation
Bioconversion of black soldier fly (Hermetia illucens) on agricultural waste: Potential source of protein and lipid, the application (A mini-review) Hidayat, Nur; Anggarini, Sakunda; Sabrina Sunyoto, Nimas Mayang; Fitri, Loeki Enggar; Suhartini, Sri; Rohma, Novita Ainur; Elviliana, Elviliana; Arif Setyawan, Sang Aji; Subekti, Indah Fitriana; Namira, Anggi Alya; Nafi’ah, Riris Waladatun; Afifah, Firdiani Nur; Pratama, Andhika Putra Agus
Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Vol 7, No 1 (2024)
Publisher : Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.afssaae.2024.007.01.8

Abstract

Hermetia illucens, well-known as black soldier fly (BSF), is an insect easily found in subtropical and tropical regions. It contains high protein and lipids. BSF is known as one of the biological agents consuming organic components, thus having a high potential to overcome organic waste problems. BSF has promising advantages due to its long development time in the larval stage (compared to other flies) and its ability to self-separate from organic waste. BSF with large protein and lipid content can substitute the commonly used protein source in aquaculture, poultry and livestock compound diet formulation, which can be an option to overcome limited sources of future food and feed insecurity. This review analyses the latest study of bioconversion using BSF from the viewpoint of nutrient composition, degradation rate and biomass results from different feed treatments. Various feed and growth mediums have been studied to obtain high protein and lipid biomass. Hopefully, the information will provide new research directions and solutions for converting agro-industrial waste using bioconversion with BSF.
Serum CXCL9 and CXCL10 as Emerging Biomarkers in Ocular Toxoplasmosis: Bridging Inflammation and Diagnostic Precision putra, prasaundra triantoni; Sofia, Ovi; Fitri, Loeki Enggar
International Journal of Retina Vol 9 No 1 (2026): International Journal of Retina (IJRetina) - INAVRS
Publisher : Indonesian Vitreoretinal Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35479/ijretina.2026.vol009.iss001.338

Abstract

Ocular toxoplasmosis (OT), the most prevalent cause of posterior uveitis worldwide, frequently results in severe visual impairment due to infections to Toxoplasma gondii. Chemokines CXCL9 and CXCL10, induced by interferon-gamma (IFN-γ), are key mediators in directing T cells and monocytes to infected ocular tissue. This review synthesizes current evidence on the role of serum CXCL9 and CXCL10 in OT pathogenesis, highlighting their involvement in immune cell trafficking, the CXCR3 receptor pathway, and the balance between host defense and retinal damage. We critically examine differences in chemokine levels between acute and chronic phases, as well as their correlation with disease activity, across human and experimental studies. Additionally, their potential as biomarkers for diagnosis and monitoring in both congenital and acquired OT is discussed. Elevated serum levels during active infection suggest clinical relevance, warranting further investigation through longitudinal and mechanistic studies to guide future therapeutic strategies.
Pengaruh Pemberian Kalsium terhadap Pertumbuhan Plasmodium falciparum in Vitro Asfirizal, Verry; Soebaktiningsih, Soebaktiningsih; Sudjari, Sudjari; Sumarno, Sumarno; Fitri, Loeki Enggar
The Journal of Experimental Life Science Vol. 1 No. 1 (2011)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (735.63 KB) | DOI: 10.21776/ub.jels.2011.001.01.03

Abstract

Peningkatan permeabilitas sel eritrosit yang terinfeksi Plasmodium falciparum terhadap ion dan makromolekul diketahui sebagai mekanisme parasit untuk memenuhi nutrisi dalam proses pertumbuhan. Peningkatan permeabilitas terhadap kalsium masih merupakan hal yang kontradiktif dalam peranannya meningkatkan pertumbuhan Plasmodium falciparum dalam sel eritrosit. Tujuan dari penelitian ini adalah mengetahui peningkatan pertumbuhan Plasmodium falciparum dalam sel eritrosit pasca pemberian kalsium. Biakan primer Plasmodium falciparum dalam medium biakan RPMI 1640 yang menghasilkan parasitemia 15%, dilakukan inokulasi untuk pembuatan subkultur yang menghasilkan parasitemia 2% dan dilakukan pembagian untuk kelompok perlakuan pemberian kalsium dan kontrol (ML 10%) dengan replikasi 3 kali. Pengamatan dilakukan hari pertama sampai hari ke-6 setelah perlakuan. Pengamatan pertumbuhan dilakukan dengan parameter parasitemia, bentuk skizon, hemolisis dan kalsium intraseluler. Hasil penelitian menunjukkan bahwa pemberian kalsium menghasilkan peningkatan tertinggi jumlah total rerata parasitemia (11,09 ± 4,01) (Rerata ± SD), bentuk skizon (23,52 ± 10,83), hemolisis (0,278 ± 0,03) dan kalsium intraseluler (6,55 ± 1,88), dibandingkan dengan media biakan kontrol (ML 10%). Analisis T-test (α= 0,05) menghasilkan perbedaan yang signifikan pada parameter parasitemia, bentuk skizon, hemolisis tetapi tidak memberikan perberbedaan yang signifikan pada parameter kalsium intraseluler. Kata kunci: glukosa, hemolisis, kalsium, kalsium intraseluler, parasitemia, skizon
Co-Authors A.A. Ketut Agung Cahyawan W AA Sudharmawan, AA Adilah Ulfiati Adilah Ulfiati, Adilah Agustin Iskandar Agustina Tri Endharti Agustina Tri Endharti Ahmad Fauziansyah Zian Ajeng Maharani Putri Alfian Wika Cahyono Ali Rahmanto Anak Agung Gede Sugianthara Andi Arahmadani Arasy Anik Widijanti Arasy, Andi Arahmadani Ardhian Wardana Ardhian Wardana, Ardhian Ariel, Dio Giovanni Arif Setyawan, Sang Aji Arifin, Mochammad Samsul Astutik Pudjirahaju Bagus Hermansyah BR. Sijabat, Melda Fio Flora Bramantyo Aji Wicaksono Bramantyo Aji Wicaksono, Bramantyo Aji Brigitta Ida RV Corebima Burhan, Niniek Cahyono, Alfian Wika Chairul A. Nidom Chairul A. Nidom Dalhar, Mochamad Damayanti, Ria Dara Dasawulansari Syamsuri Desy Andari Dewi Indiastari Didi Candradikusuma Didi Candradikusuma Dio Giovanni Ariel Dorta Simamora Edi Widjajanto Elviliana, Elviliana Erma Sulistyaningsih Erwan, Nabila Erina Eviana Norahmawati Firdiani Nur Afifah, Firdiani Nur Fitria Febriliani Putri Fitriana Nugraheni Fitriana Nugraheni, Fitriana Ghofar, Hervin Febrina Handono Kalim Hayu Sukowati Nopitasari Heri Kristianto Heri Suroso, Heri Herpan Syafii Harahap Hervin Febrina Ghofar Hidayat Sujuti Husnul Asariati Husnul Asariati I Ketut Gede Muliartha Ihwan Ihwan Ika Setyo Rini Irene Ratridewi Isnadiyah Juhdi Joko Agus Gunawan Josef Sem Berth Tuda Juhdi, Isnadiyah Kana Mardhiyyah Kartika Senjarini Karyono Mintaroem Karyono Mintaroem Karyono Mintaroem Khadafi Indrawan Khairiyadi Khairiyadi Krisni Subandiyah Krisni Subandiyah Krisni Subandiyah Krsni Subandiyah Kusworini Handono Laksmi Karunia Tanuwijaya Laksmi Karunia Tanuwijaya Lilik Maslachah Lilik Zuhriyah M. Fahrul Arifin Mardhiyyah, Kana Mario B. Nara Melda Fio Flora BR. Sijabat Merici, Angela Mochammad Dalhar Moh Mirza Nuryady Moh Mirza Nuryady, Moh Mirza Mohammad Saifur Rahman Mulyohadi Ali Muti'ah, Roihatul Nabila Erina Erwan Nafi’ah, Riris Waladatun Namira, Anggi Alya Nashi Widodo Natalia Erica Jahja Nicole Berens-Riha Nicole Berens-Riha, Nicole Niniek Budiarti B Niniek Burhan Noer Aini Nopitasari, Hayu Sukowati Nugraha, Rivo Yudhinata Brian Nur Fahma Pradiptasari Nur Fahma Pradiptasari, Nur Fahma Nur Hidayat Nur Permatasari Nurdiana Nurdiana Nurdiana Nurdiana Nurtyas, Yunika pertiwi, resti anggun Poedji Hastutiek Pratama, Andhika Putra Agus putra, prasaundra triantoni Putri, Ajeng Maharani Putri, Fitria Febriliani Putri, Rachmania Rachmania Putri Rahma, Zainabur Rahmad Rahmad Rahman, Mohammad Saifur Renny Suwarniaty Renny Suwarniaty, Renny Rifa'i, Muhaimin Rivo Yudhinata Brian Nugraha Rizky Amalia Rohma, Novita Ainur Sabrina Sunyoto, Nimas Mayang Saiful Akhyar Lubis Sakunda Anggarini, Sakunda Samsul Arifin Siti Candra Windu Baktiyani Siti Candra Windu Baktiyani Soebaktiningsih Soebaktiningsih Soemarko Soemarko Sofia, Ovi Sri Hayati, Yati Sri Murwani Sri Poeranto, Sri Sri Suhartini Sri Winarsih Subekti, Indah Fitriana Sudjari Sudjari Sugeng Setyo Utomo Sugeng Setyo Utomo, Sugeng Setyo Sujarot Dwi Sasmito sujarot dwi sasmito, sujarot dwi Sumarno . Susanto Nugroho Susanto Nugroho Sutiman B. Sumitro Swastomo, Rahadi Syarkiah Syarkiah Teguh Wahju Sardjono Teguh Wahju Sardjono Teguh Wahju Sardjono Thomas Loescher Thomas Loescher, Thomas Tikto Wahyono Tinny Endang Endang H Tinny Endang Hernowati Tinny Endang Hernowati Tuda, Josef Sem Berth Umar Zein Verry Asfirizal Vonny Mariany Deckert Wahyono, Tikto Wongso Suhendro Yoes Prijatna Dachlan Yuanita Mulyastuti Yuliyanik yuliyanik Yunika Nurtyas Yunita Armiyanti Zainabur Rahma Zakiyah Zulaifa Zakiyah Zulaifa, Zakiyah