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2. Anti-Bacterial Activity Of N-Hexane Extract Of Malacca Leave (Phyllanthus emblica) On Mice (Mus musculus) Inoculated By Staphylococcus epidermidis In Vivo Nada Sarah Syahputri; Nuzul Asmilia; Rinidar Rinidar; Amalia Sutriana; Fakhrurrazi Fakhrurrazi; Ginta Riady
Jurnal Medika Veterinaria Vol 15, No 1 (2021): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v15i1.19840

Abstract

Malacca plant (Phyllanthus emblica) is one of the medicinal plants. The purpose of this study was to determine the effect of n-hexane extract of Malacca (Phyllanthus emblica) leaves on the growth of Staphylococcus epidermidis bacteria in vivo. All mice were first induced by Staphylococcus epidermidis bacteria. Negative control (K1) was given aquadest, positive control (K2) was given ciproflaxacin suspension at doses of 20 mg/kg BW, while K3, K4, and K5 were given n-hexane extract of Malacca leave at dose of 100 mg/kg BW, 200 mg/kg BW, and 300 mg/kg BW. Respectively blood sampling was carried out on the 5th day after treatment. Data were analyzed using one-way analysis of variance (ANOVA). The results showed that the mean (± SD) number of bacterial colonies in K1 was 656x10² cfu/ml. The average number of bacterial colonies in K2 was 2328x10² cfu/ml. The average number of bacterial colonies given n-hexane extract of malacca leave 100 mg/kg BW on K3 was 359,60x10² cfu/ml. The average number of bacterial colonies given n-hexane extract of malacca leave 200 mg/kg BW at K4 was 200x10² cfu/ml and the average number of bacterial colonies given n-hexane extract of malacca leave 300 mg/kg BW at K5 was 3483x10² cfu/ml. The results showed there were no significant difference among treatment groups (P 0.05). N-hexane extract of malacca leave was unable to inhibit the growth of Staphylococcus epidermidis bacteria in vivo
Amelioration of Seminal Plasma Testosterone Concentration in Gembrong Goats after In Vivo Administration of PGF2α Hafizuddin, Hafizuddin; Husnurrizal, Husnurrizal; Siregar, Tongku Nizwan; Eriani, Kartini; Wahyuni, Sri; Ahsan, Muhammad Maulana; Sutriana, Amalia; Anwar, Anwar; Aliza, Dwinna
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.256-261

Abstract

The semen quality of Gembrong goats is lower than other goats and may be related to the low concentration of testosterone hormone. Implementation of reproductive technology using prostaglandin F2α (PGF2α) hormone is beneficial to increase the testosterone hormone in Gembrong goats. This study aimed to determine the effect of PGF2α injection on increasing testosterone levels in Gembrong goats. Male Gembrong goats (n=4), aged 2.5-4 years with similar body condition scores (BCS=3) were used in this study. Goats were divided into two treatment groups (n=2). Goats in group 1 (G1) were injected intramuscularly with 1 ml PGF2α (75 μg), while those in group 2 (G2) were injected with 1 ml physiological NaCl. Semen collection was carried out 30 minutes after treatment using an artificial vagina. Testosterone levels were measured using the enzyme-linked immunosorbent assay (ELISA). The collected data was tabulated and analyzed descriptively. The results showed that the average testosterone concentration of G1 was higher than G2 with respective concentrations of 6.41 ± 0.70 and 2.81 ± 1.75 ng/ml. It was concluded that administration of PGF2α in vivo could increase testosterone concentration in Gembrong goats.
Toxicity Test of Flavonoid Compounds from Ethyl Acetate Extract of Malacca Leaves with Brine Shrimp Lethality Test Zakwan, Muhammad; Sutriana, Amalia; Nurliana, Nurliana; Asmilia, Nuzul; Ammar, Muhammad; Novianti, Arindita Niatazya
Jurnal Medik Veteriner Vol. 6 No. 3 (2023): Special Issue: International Conference on Veterinary Medicine and Health Scien
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss3.2023.15-19

Abstract

Flavonoids are a group of polyphenolic compounds produced in plants as secondary metabolites. Flavonoids have favorable biochemical effects on multiple diseases and other bioactivities. This study aimed to isolate the active compounds contained in malacca (Phyllanthus emblica) leaves and analyze toxic properties using the Brine Shrimp Lethality Test (BSLT) method in Artemia salina leach shrimp larvae. This study used malacca leaf powder with ethyl acetate solvent then macerated. Ethyl acetate extract was concentrated and fractionated, then toxicity tests were carried out. Extracts from the evaporation results were further fractionated using solvent petroleum ether, diethyl ether, and ethyl acetate. Then the ethyl acetate fraction was hydrolyzed using reflagged with 7% sulfuric acid for two hours, then the filtrate was extracted with ethanol solvent. The obtained fraction washed with aquades and dried using a vacuum desiccator. The toxicity test results showed that the flavonoid compound of malacca leaves was not toxic with a value of LC50 > 1000 ppm, an increase in the concentration of the extract was followed by an increase in the average mortality of larvae.
Antibacterial and anti-inflammatory activities of Nothopanax scutellarium, Moringa oleifera and Piper betle extracts on staphylococcal mastitis animal model Sriyanti, Cut; Siregar, Tongku N.; Mudatsir, Mudatsir; Gani, Azhari; Hasan, Denny I.; Sutriana, Amalia
Narra J Vol. 3 No. 2 (2023): August 2023
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v3i2.176

Abstract

Inappropriate and prolonged administration of antibiotics in mastitis could cause antibiotic resistance and herbal treatment might could be one alternative treatment. Nothopanax scutellarium, Moringa oleifera, and Piper betle are medicinal plants that contain various active compounds, including antibacterial and anti-inflammatory agents, but their potentials in treating mastitis are not well understood. The aim of this study was to assess the effectiveness of those plants against mastitis in rabbit model induced by Staphylococcus aureus. A total of 25 lactating rabbits (Oryctolagus cuniculus) weighing 3.0±0.4 kg were grouped into five groups: healthy control; mastitis control, and three treatment groups (Nothopanax scutellarium, Moringa oleifera, and Piper betle). Except the healthy control group, all animals were inoculated with 0.15 mL of Staphylococcus aureus containing 1.5x107colony forming unit (CFU)/mL on eight days after giving birth. The extract was administered orally after four hours Staphylococcus aureus inoculation at a dose of 50 mg/kg body weight, twice a day for five consecutive days. The number of bacteria in the milk and the level of serum interleukin 6 (IL-6) were measured and histopathological examination of mammary gland tissues were analyzed. The log number of total plate count of Staphylococcus aureus indicated that all extract groups had significant lower of bacterial logs compared to mastitis control (all comparisons had p<0.05) with the lowest was found in Piper betle group, followed by Nothopanax scutellarium and Moringa oleifera. The enzyme-linked immunosorbent assay (ELISA) results showed that all ethanolic extract groups had significantly lower levels of IL-6 compared to the mastitis control (all comparisons had p<0.05). The histopathologyassessment suggested that extract groups had lower infiltration of inflammatory cells such as lymphocytes and macrophages in alveoli compared to the mastitis control group. In conclusion, all three extracts contained antibacterial and anti-inflammatory activities and Piper betle had the most effective in reducing bacterial growth and IL-6 level compared to others.
Pembuatan jerami terfermentasi probion sebagai pakan alternatif pada ternak sapi terintegrasi di Gampong Blang Krueng Kecamatan Baitussalam Kabupaten Aceh Besar [Making probion fermented straw as an alternative feed in integrated cattle farming in Blang Krueng Village, Baitussalam Sub-District, Aceh Besar District] Sutriana, Amalia; Hanafiah, Muhammad; Melia, Juli; Aliza, Dwinna
Buletin Pengabdian Vol 4, No 3 (2024): Bull. Community. Serv.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/bulpen.v4i3.41100

Abstract

Farmers, especially those in Blang Krueng Village, Baitussalam Subdistrict, Aceh Besar District, have so far been raising livestock using traditional or semi-extensive patterns, where livestock are released in pastures or rice fields to find their own feed and in the afternoon given grass that has been cut into pieces. The purpose of the activity is to assist farmers in their livestock business, especially in the problem of feed needs that still cannot be met. This activity is carried out using the PRA (Participatory Rural Appraisal) approach. In this case the target audience participates in increasing and analyzing their knowledge about their own conditions so that they can make plans and actions, especially in efforts to raise cattle. The method of processing fermented straw, among others, the straw is aerated so that the moisture content is 40%, the straw is stacked with a length of 2.5 m, a width of 2.5 m and a thickness of 25 cm, on top of the straw layer watered with water that has been mixed with urea until evenly distributed, on top of the straw layer sprinkled with starter until evenly distributed, the straw is stacked again with a thickness of 25 cm trampled until solid, repeated watering of water mixed with urea until evenly distributed, repeated sowing of starter until evenly distributed, so repeated until the pile can reach 3 m, after completion the top is covered with dry leaves such as banana leaves, rice straw is left for at least 3-4 weeks and fermented rice straw is ready to be given to livestock. The results of the service that have been achieved are the availability of alternative feed from fermented straw and can be given to cattle, where the cattle also want to consume it.
The Effectiveness of Lugol on the Increasing of Pregnancy Rate in Aceh Cow with Endometritis Amalia Sutriana; Arman Sayuti; Budianto Panjaitan; Teuku Armansyah TR; Aisyah Fadillah Tunnisa; Juli Melia; Tongku Nizwan Siregar; Hafizuddin Hafizuddin; Dwinna Aliza
Jurnal Agripet Vol 21, No 2 (2021): Volume 21, No. 2, Oktober 2021
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v21i2.18513

Abstract

ABSTRACT. The objective of this study was to determine the effect of lugol on the increasing the pregnancy rate in repeat breeding (RB) Aceh cows due to endometritis. This study used six endometritiss cows, aged 5-7 years, weighed 150-250 kg which were divided into two groups (n=3), KI and KII. The cows in group 1 (K1) were injected with 5 ml PGF2, while the cows in group 2 (KII) were treated with 50 ml of 2% lugol intra-uterine and continued with an injection of 5 ml PGF2 after healing. The detection of estrus was performed twice a day following by artificial insemination (AI) about 10-16 hours after the onset of estrus. Determination of pregnancy was performed by ultrasonography (USG) on the 25th day after AI. The data obtained were analyzed descriptively. The results showed that all endometritis cows in KI and KII present estrous signs (100%). However, only one cow was recovered in K2, whereas in K1 did not. After AI, one pregnant cow was observed in KII (33.3%), while none of the pregnant cows was found in K1 (0.0%). It is concluded that the lugol treatment for endometritiss Aceh cows can improve the pregnancy rate.(Efektivitas larutan lugol untuk meningkatkan persentase kebuntingan pada sapi Aceh yang mengalami endometritis) ABSTRAK. Penelitian ini bertujuan mengetahui pengaruh pemberian larutan lugol terhadap peningkatan persentase kebuntingan sapi Aceh yang mengalami RB. Dalam penelitian ini digunakan enam ekor sapi Aceh betina dewasa, umur 5-7 tahun, bobot badan 150-250 kg, sudah pernah beranak, dan didiagnosis mengalami endometritis. Seluruh sapi dibagi menjadi dua kelompok (n=3). Pada kelompok 1 (K1), sapi endometritis diterapi dengan 50 ml lugol 2% secara intra uteri dan setelah sembuh dilanjutkan dengan penyuntikan 5 ml PGF2. Sapi pada kelompok 2 (K2) hanya diinjeksi dengan 5 ml PGF2. Deteksi berahi dilakukan sebanyak dua kali per hari dan inseminasi buatan (IB) dilakukan sekitar 10-16 jam setelah awal berahi. Pemeriksaan kebuntingan dilakukan dengan ultrasonografi (USG) pada hari ke-25 setelah IB. Data dianalisis secara deskriptif. Dari masing-masing kelompok, hanya satu ekor sapi pada K2 yang dinyatakan sembuh yaitu sapi pada kelompok kedua. Persentase sapi yang menjadi estrus pada kedua kelompok masing-masing adalah 100%. Dari tiga ekor sapi yang diinseminasi pada masing-masing kelompok, hanya satu ekor sapi pada K2 (33,3%) yang menunjukkan hasil positif bunting sedangkan pada K1 tidak terdapat sapi yang menunjukkan hasil positif (0,0%). Disimpulkan bahwa pemberian larutan lugol pada sapi Aceh yang mengalami endometritis dapat meningkatkan persentase kebuntingan sapi Aceh.
Potential of ant nest extract (Hydnophytum formicarum) for protection of testicular morphometry, epididymal functions, and sperm quality in male rats with alloxan-induced diabetes Roslizawaty, Roslizawaty; Abrar, Mahdi; Khairan, Khairan; Gholib, Gholib; Siregar, Tongku N.; Syafruddin, Syafruddin; Sutriana, Amalia; Azzahra, Putri; Aini, Hanipah
Narra J Vol. 4 No. 3 (2024): December 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i3.922

Abstract

Medicinal herbs, such as the ant nest plant (Hydnophytum formicarum), are promising for the management of diabetes mellitus-associated infertility. The aim of this study was to evaluate the biological activity of the ant nest plant and its capacity to mitigate the adverse effects of alloxan-induced diabetes on testicular morphology, epididymal function, and sperm quality in male rats. The tuber of the ant nest plant was extracted using methanol and then subjected to phytochemical screenings. For the experiment, 20 male white rats (Rattus norvegicus), aged 3–4 months and weighing 150–200 g, were equally divided into four groups. The ant nest extract was administered orally using oral gavage over 14 days. The testes, epididymis, and sperm were collected for weighing, morphometric measurements, and quality evaluation. Qualitative testing of phytochemical compounds indicated the presence of flavonoids, tannins, steroids, terpenoids, and phenolic compounds in the plants. The results revealed the protective effects of ant nest extract against the adverse fertility effects induced by alloxan and a high-fat diet, as observed in testicular weight (p=0.045), epididymal weight (p=0.041), and sperm quality (p>0.05).
Hormonal induction of gonad maturation in female tinfoil barb fish (Barbonymus schwanenfeldii) using spawnprim hormone Amalia Sutriana; Ahmad Baihaqi; Iwan Hasri; Nurliana; Hafizuddin Hafizuddin
Jurnal Akuakultur Indonesia Vol. 21 No. 2 (2022): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.21.2.118-124

Abstract

The aim of this study was to analyze the effect of spawnprim hormone induction with different doses on gonadal maturity of tinfoil barb fish broodstock (Barbonymus schwanenfeldii). A completely randomized design (CRD) was used in this study with 4 treatments and 3 replications. Fish broodstock in group A (control) was not given any treatment, while fish broodstocks in groups B, C, and D were injected with spawnprim hormone at doses of 0.3 ml/kg body weight, 0.6 ml/kg body weight, and 0.9 ml/kg body weight, respectively. The measured parameters were gonad maturity level, broodstock weight gain, egg diameter, and fecundity. Data were analyzed using one way analysis of variance (ANOVA). The result showed that the gonad maturity level (GML) in groups B, C, and D (GML III and IV) was better than group A (GML I). Statistical analysis showed that the induction of spawnprim hormone on tinfoil barb broodstocks significantly affect (P<0.05) broodstock weight gain, egg diameter, and fecundity. The optimal dose of spawnprim hormone for tinfoil barb was 0.6 ml/kg body weight, with the average broodstock weight gain, egg diameter, and fecundity were 0.011 kg, 1.55 mm, and 102.15 eggs, respectively. The induction of spawnprim hormone has a positive effect on gonad maturation of tinfoil barb fish (B. schwanenfeldii), with the optimum dose is 0.6 ml/kg fish Keywords: gonad maturity, tinfoil barb fish, spawnprim ABSTRAK Penelitian ini bertujuan untuk menganalisis pengaruh penggunaan hormon spawnprim dengan dosis yang berbeda terhadap kematangan gonad induk ikan lemeduk Barbonymus schwanenfeldii. Metode yang digunakan pada penelitian ini adalah metode rancangan acak lengkap (RAL) dengan 4 kelompok perlakuan dan 3 kali pengulangan. Ikan pada kelompok A (kontrol) tidak diberikan perlakuan, sedangkan ikan pada kelompok B, C, dan D masing-masing diinjeksi hormon spawnprim dengan dosis 0.3 ml/kg bobot badan; 0.6 ml/kg bobot badan; dan 0,9 ml/kg bobot badan. Parameter yang diamati adalah persentase induk matang gonad akhir, pertambahan bobot induk, pertambahan diameter telur, dan fekunditas. Data dianalisis dengan menggunakan analisis varians (ANAVA) pola satu arah. Hasil penelitian menunjukkan tingkat kematangan gonad (TKG) pada kelompok perlakuan B, C, dan D (TKG III dan IV) lebih baik dari kelompok A (TKG I). Hasil uji ANAVA menunjukkan bahwa pemberian hormon spawnprim pada induk ikan lemeduk berpengaruh nyata (P<0.05) terhadap pertambahan bobot induk, pertambahan diameter telur, dan fekunditas. Tingkat kematangan gonad terbaik pada penelitian ini diperoleh pada kelompok perlakuan C yang diinduksi hormon spawnprim dengan dosis 0.6 ml/kg bobot badan induk ikan dengan rata - rata pertambahan bobot induk, pertambahan diameter telur, dan fekunditas masing-masing adalah 0.011 kg, 1.55 mm, dan 102.15 butir telur. Induksi hormon spawnprim menimbulkan pengaruh yang positif terhadap pertambahan bobot induk, diameter telur dan fekunditas ikan lemeduk (B. schwanenfeldii), dengan dosis optimum 0.6 ml/kg bobot badan. Kata kunci: kematangan gonad, ikan lemeduk, spawnprim
Aplikasi Metode Pemeriksaan Kimia Urin Untuk Diagnosis Kebuntingan Dini Pada Sapi Masyarakat Di Desa Lampuuk Kecamatan Kuta Baro Kabupaten Aceh Besar Syafruddin Syafruddin; Arman Sayuti; Tongku Nizwan Siregar, MP; Teuku Armansyah; Sri Wahyuni; Hafizuddin Hafizuddin; Budianto Panjaitan; Amalia Sutriana; Mulyadi Adam
Peternakan Abdi Masyarakat (PETAMAS) Vol 3, No 1 (2023): Volume 3, Nomor 1, Juni 2023
Publisher : Departemen of Animal Science, Agriculture Faculty, Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/petamas.v3i1.30921

Abstract

Kegiatan pengabdian kepada masyarakat bertujuan meningkatkan pengetahuan peternak tentang kerugian yang diakibatkan oleh ketiadaan pemeriksaan kebuntingan dan meningkatkan pengetahuan dan keterampilan inseminator tentang metode pemeriksaan kimia urin. Kelompok sasaran dari kegiatan ini adalah 15 (lima belas) orang masyarakat petani peternak sapi di Desa Lampuuk Kecamatan Kuta Baro Kabupaten Aceh Besar dan 2 (dua) orang inseminator di wilayah setempat. Pelaksanaan kegiatan ini dilakukan dengan 2 metode, yaitu (1) metode pendidikan dan penyuluhan dan (2) demonstrasi dan pelatihan di lapangan. Pada kegiatan penyuluhan, peternak akan diajarkan tentang kerugian akibat ketiadaan diagnosis kebuntingan. Kepada inseminator setempat diperkenalkan cara dan alat/bahan yang diperlukan untuk diagnosis kebuntingan. Pada kegiatan demonstrasi akan dilakukan pemeriksaan urin sapi yang telah diinseminasi buatan. Dari hasil kegiatan disimpulkan bahwa pengetahuan peternak tentang kerugian yang diakibatkan oleh ketiadaan pemeriksaan kebuntingan meningkat dan inseminator terampil melakukan diagnosis kebuntingan melalui pemeriksaan kimia urin.
Total Plate Numbers and Coliforms in The Processing of Milkfish Without Spines (Chanos Chanos) in Household Indusrty in Banda Aceh Nurliana, Nurliana; Aftari, Nailul; Sutriana, Amalia; Asmilia, Nuzul; Sari, Wahyu Eka
Jurnal Medika Veterinaria Vol 17, No 2 (2023): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v17i2.34843

Abstract

Microbial contamination of fish has the potential to cause product quality degradation and cause fishborne disease. Microbial contamination can occur at any stage of fish processing. This study aimed to determine the total plate count and coliform at each stage process of milkfish (Chanos chanos) boneless. Milkfishes were obtained from three processing stage, namely raw material (fresh fish) stage, the fish bone removal stage and the baking fish stage. The parameters observed were the number of colonies growing as total plate count and coliform as the most probable number (MPN). The results showed that the highest ALT contamination was found at the bone removal stage with an average of 1.7x104 CFU/g but this figure did not exceed the standard limit and the lowest was at the firing stage with an average of 1.2x103 CFU/g. The highest coliform was found in the fresh fish stage, namely 129 MPN/g and the lowest was 3 MPN/g. From this research, it can be concluded that the total microbial plate number does not exceed the SNI 7288: 2009 standard and the coliform exceed the SNI 7388: 2009 standard.
Co-Authors Abbas, Muhammadar Abdullah ABDELBAGI, MOHAMMED AHMED ELSHAREF Abdul Harris Abdullah A. Muhammadar Abidin, Muchlisin Zainal Aftari, Nailul Ahmad Baihaqi Ahsan, Muhammad Maulana Aini, Hanipah Aisyah Fadillah Tunnisa Ajirni Ajirni Akter, Mst Nahid Aliya, Yayang Nuri Al Amiruddin Amiruddin Amiruddin Amiruddin Annisa Anwar Sitorus Anwar Anwar Arman Sayuti Arman Sayuti Arman Sayuti Arman Sayuti Asmawati Asmawati Azzahra, Putri Batubara, Agung Setia budianto panjaitan Budianto Panjaitan Budianto Panjaitan Budianto Panjaitan Budianto Panjaitan Budianto Panjaitan Cut Nila Thasmi Cut Nila Thasmi, Cut Nila D Dasrul Darmawi Darmawi Dasrul Dasrul Dasrul, D Dedi Fazriansyah Putra Dian Masyitha Dwina Aliza Dwinna Aliza Dwinna Aliza Dwinna Aliza Elza Savitri Erdiansyah Rahmi Erdiansyah Rahmi Erdiansyah Rahmi Erina Erina Erina Erina Fakhrurrazi Fakhrurrazi Fakhrurrazi Fakhrurrazi Farid Wajidi Farida Farida Feby Fema Amzani Nasution Firdus Firdus Firdus Firdus Fuza Khoiriah Gani, Azhari Gholib Gholib, Gholib Ginta Riady Ginta Riady Hafizuddin Hafizuddin Hafizuddin Hafizuddin HAFIZUDDIN, UMAR PUTEH Hamdan Hamdan Hamdan Hamdan HANUM, FARAH FARIDA Hasan, Denny I. Henni Vanda HENNIVANDA HENNIVANDA Herrialfian . Herrialfian, Herrialfian Husnurrizal . Husnurrizal, Husnurrizal Indriany, Sudi Ismail Ismail Iwan Hasri Juli Melia Juli Melia KARTINI ERIANI Karunita Dewi Khairan Khairan Mada Admi Mahdi Abrar Masda Admi Mentari Azhari L Mira Ayu Lestari Hasibuan Mohd Nor, Siti Azizah Mst Nahid Akter Muchlisin Zainal Abidin Mudatsir Mudatsir Muhammad Ammar Muhammad Hambal Muhammad Hambal Muhammad Hanafiah Muhammad Hanif Muhammad Isa Muhammad Reza Mulyadi Adam Mulyadi Adam, Mulyadi Nada Sarah Syahputri Nazaruddin Nazaruddin Nazaruddin Nazaruddin Novianti, Arindita Niatazya Nurhazimah Nurhazimah Nurliana NURLIANA NURLIANA Nuzul Asmilia Nuzul Asmilia Nuzul Asmilia Putra, Dedi Fazriansyah Qathrinnada Ramadhana R Roslizawaty R Roslizawaty, R Rahmad Rahmad Rasmaidar . Raysa Syarifah Razali Daud Razali Daud rina aulia barus Rinidar Rinidar Roshada Hashim Roshada Hashim Roslizawaty Roslizawaty Roslizawaty Roslizawaty Roslizawaty Roslizawaty, Roslizawaty Rosmaidar Rosmaidar Rosmaidar Rosmaidar, Rosmaidar Rossy Septia Putri S Syafruddin Sail, Asmawati Muhammad Sari, Wahyu Eka Sembiring, Rinawati Siddik, Tasya Luthfiah Siregar, Tongku N. Siti Aisyah Siti Azizah Mohd Nor Sri Wahyuni Sri Wahyuni Sriyanti, Cut Sudi Indriany Sugito Sugito Syafruddin Syafruddin Syafruddin Syafruddin Syafruddin Syafruddin Syafruddin, S T Armansyah TR T. Armansyah T. Armansyah T. Armansyah Teuku Armansyah Teuku Armansyah Titit, Marcelus Ferdinandez Tongku N. Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar, MP Triva Murtina Lubis Winaruddin Winaruddin Yayang Nuri Al Aliya Yusmadi Yusmadi Zakwan, Muhammad