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1. The Sensitivity Level Of Gentamicine, Cholramphenicol and Penicillin Inhibiting The Growth Of Pseudomonas Aeruginosa Bacteria Isolate From Aceh Bull Prepunce Mada Admi; Annisa Anwar Sitorus; Rinidar Rinidar; Amalia Sutriana; Rosmaidar Rosmaidar; Sugito Sugito
Jurnal Medika Veterinaria Vol 15, No 1 (2021): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v15i1.20856

Abstract

This study aims to determine the sensitivity level of gentamicin, chloramphenicol and penicillin antibiotics to inhibit the bacterial growth of P. aeruginosa taken from preputium isolate of Aceh cattle. This Stock of Pseudomonas aeruginosa isolate from Microbiology Laboratory of FKH USK, re-identified its purity through: indole test, methyl red test, sugar test and as well as on Nutrient Broth (NB) media; incubated at 37ºC for 24 hours; observed the turbidity level of 0.5 Mc Farland solution. Then, the antibiotic sensitivity test was carried out using the method of Mueller Hinton Agar (MHA). The observations were made by measure the inhibition zone using the calipers in millimeters (mm) against antibiotics. The results of the observation of the inhibition zone on the gentamicin antibiotic was averaged of 25.5 mm, 23.7 mm chloramphenicol, and 12.1 mm penicillin. This study concluded that the gentamicin, chloramphenicol, and penicillin antibiotic were still effective against P. aeruginosa bacteria with the highest level of sensitivity seen in gentamicin antibiotic. 
2. Anti-Bacterial Activity Of N-Hexane Extract Of Malacca Leave (Phyllanthus emblica) On Mice (Mus musculus) Inoculated By Staphylococcus epidermidis In Vivo Nada Sarah Syahputri; Nuzul Asmilia; Rinidar Rinidar; Amalia Sutriana; Fakhrurrazi Fakhrurrazi; Ginta Riady
Jurnal Medika Veterinaria Vol 15, No 1 (2021): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v15i1.19840

Abstract

Malacca plant (Phyllanthus emblica) is one of the medicinal plants. The purpose of this study was to determine the effect of n-hexane extract of Malacca (Phyllanthus emblica) leaves on the growth of Staphylococcus epidermidis bacteria in vivo. All mice were first induced by Staphylococcus epidermidis bacteria. Negative control (K1) was given aquadest, positive control (K2) was given ciproflaxacin suspension at doses of 20 mg/kg BW, while K3, K4, and K5 were given n-hexane extract of Malacca leave at dose of 100 mg/kg BW, 200 mg/kg BW, and 300 mg/kg BW. Respectively blood sampling was carried out on the 5th day after treatment. Data were analyzed using one-way analysis of variance (ANOVA). The results showed that the mean (± SD) number of bacterial colonies in K1 was 656x10² cfu/ml. The average number of bacterial colonies in K2 was 2328x10² cfu/ml. The average number of bacterial colonies given n-hexane extract of malacca leave 100 mg/kg BW on K3 was 359,60x10² cfu/ml. The average number of bacterial colonies given n-hexane extract of malacca leave 200 mg/kg BW at K4 was 200x10² cfu/ml and the average number of bacterial colonies given n-hexane extract of malacca leave 300 mg/kg BW at K5 was 3483x10² cfu/ml. The results showed there were no significant difference among treatment groups (P 0.05). N-hexane extract of malacca leave was unable to inhibit the growth of Staphylococcus epidermidis bacteria in vivo
Amelioration of Seminal Plasma Testosterone Concentration in Gembrong Goats after In Vivo Administration of PGF2α Hafizuddin, Hafizuddin; Husnurrizal, Husnurrizal; Siregar, Tongku Nizwan; Eriani, Kartini; Wahyuni, Sri; Ahsan, Muhammad Maulana; Sutriana, Amalia; Anwar, Anwar; Aliza, Dwinna
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.256-261

Abstract

The semen quality of Gembrong goats is lower than other goats and may be related to the low concentration of testosterone hormone. Implementation of reproductive technology using prostaglandin F2α (PGF2α) hormone is beneficial to increase the testosterone hormone in Gembrong goats. This study aimed to determine the effect of PGF2α injection on increasing testosterone levels in Gembrong goats. Male Gembrong goats (n=4), aged 2.5-4 years with similar body condition scores (BCS=3) were used in this study. Goats were divided into two treatment groups (n=2). Goats in group 1 (G1) were injected intramuscularly with 1 ml PGF2α (75 μg), while those in group 2 (G2) were injected with 1 ml physiological NaCl. Semen collection was carried out 30 minutes after treatment using an artificial vagina. Testosterone levels were measured using the enzyme-linked immunosorbent assay (ELISA). The collected data was tabulated and analyzed descriptively. The results showed that the average testosterone concentration of G1 was higher than G2 with respective concentrations of 6.41 ± 0.70 and 2.81 ± 1.75 ng/ml. It was concluded that administration of PGF2α in vivo could increase testosterone concentration in Gembrong goats.
Toxicity Test of Flavonoid Compounds from Ethyl Acetate Extract of Malacca Leaves with Brine Shrimp Lethality Test Zakwan, Muhammad; Sutriana, Amalia; Nurliana, Nurliana; Asmilia, Nuzul; Ammar, Muhammad; Novianti, Arindita Niatazya
Jurnal Medik Veteriner Vol. 6 No. 3 (2023): Special Issue: International Conference on Veterinary Medicine and Health Scien
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss3.2023.15-19

Abstract

Flavonoids are a group of polyphenolic compounds produced in plants as secondary metabolites. Flavonoids have favorable biochemical effects on multiple diseases and other bioactivities. This study aimed to isolate the active compounds contained in malacca (Phyllanthus emblica) leaves and analyze toxic properties using the Brine Shrimp Lethality Test (BSLT) method in Artemia salina leach shrimp larvae. This study used malacca leaf powder with ethyl acetate solvent then macerated. Ethyl acetate extract was concentrated and fractionated, then toxicity tests were carried out. Extracts from the evaporation results were further fractionated using solvent petroleum ether, diethyl ether, and ethyl acetate. Then the ethyl acetate fraction was hydrolyzed using reflagged with 7% sulfuric acid for two hours, then the filtrate was extracted with ethanol solvent. The obtained fraction washed with aquades and dried using a vacuum desiccator. The toxicity test results showed that the flavonoid compound of malacca leaves was not toxic with a value of LC50 > 1000 ppm, an increase in the concentration of the extract was followed by an increase in the average mortality of larvae.
Antibacterial and anti-inflammatory activities of Nothopanax scutellarium, Moringa oleifera and Piper betle extracts on staphylococcal mastitis animal model Cut Sriyanti; Siregar, Tongku N.; Mudatsir, Mudatsir; Gani, Azhari; Hasan, Denny I.; Sutriana, Amalia
Narra J Vol. 3 No. 2 (2023): August 2023
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v3i2.176

Abstract

Inappropriate and prolonged administration of antibiotics in mastitis could cause antibiotic resistance and herbal treatment might could be one alternative treatment. Nothopanax scutellarium, Moringa oleifera, and Piper betle are medicinal plants that contain various active compounds, including antibacterial and anti-inflammatory agents, but their potentials in treating mastitis are not well understood. The aim of this study was to assess the effectiveness of those plants against mastitis in rabbit model induced by Staphylococcus aureus. A total of 25 lactating rabbits (Oryctolagus cuniculus) weighing 3.0±0.4 kg were grouped into five groups: healthy control; mastitis control, and three treatment groups (Nothopanax scutellarium, Moringa oleifera, and Piper betle). Except the healthy control group, all animals were inoculated with 0.15 mL of Staphylococcus aureus containing 1.5x107colony forming unit (CFU)/mL on eight days after giving birth. The extract was administered orally after four hours Staphylococcus aureus inoculation at a dose of 50 mg/kg body weight, twice a day for five consecutive days. The number of bacteria in the milk and the level of serum interleukin 6 (IL-6) were measured and histopathological examination of mammary gland tissues were analyzed. The log number of total plate count of Staphylococcus aureus indicated that all extract groups had significant lower of bacterial logs compared to mastitis control (all comparisons had p<0.05) with the lowest was found in Piper betle group, followed by Nothopanax scutellarium and Moringa oleifera. The enzyme-linked immunosorbent assay (ELISA) results showed that all ethanolic extract groups had significantly lower levels of IL-6 compared to the mastitis control (all comparisons had p<0.05). The histopathologyassessment suggested that extract groups had lower infiltration of inflammatory cells such as lymphocytes and macrophages in alveoli compared to the mastitis control group. In conclusion, all three extracts contained antibacterial and anti-inflammatory activities and Piper betle had the most effective in reducing bacterial growth and IL-6 level compared to others.
Pembuatan jerami terfermentasi probion sebagai pakan alternatif pada ternak sapi terintegrasi di Gampong Blang Krueng Kecamatan Baitussalam Kabupaten Aceh Besar [Making probion fermented straw as an alternative feed in integrated cattle farming in Blang Krueng Village, Baitussalam Sub-District, Aceh Besar District] Sutriana, Amalia; Hanafiah, Muhammad; Melia, Juli; Aliza, Dwinna
Buletin Pengabdian Bulletin of Community Services Vol 4, No 3 (2024): Bull. Community. Serv.
Publisher : The Institute for Research and Community Services (LPPM) Universitas Syiah Kuala (USK)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/bulpen.v4i3.41100

Abstract

Farmers, especially those in Blang Krueng Village, Baitussalam Subdistrict, Aceh Besar District, have so far been raising livestock using traditional or semi-extensive patterns, where livestock are released in pastures or rice fields to find their own feed and in the afternoon given grass that has been cut into pieces. The purpose of the activity is to assist farmers in their livestock business, especially in the problem of feed needs that still cannot be met. This activity is carried out using the PRA (Participatory Rural Appraisal) approach. In this case the target audience participates in increasing and analyzing their knowledge about their own conditions so that they can make plans and actions, especially in efforts to raise cattle. The method of processing fermented straw, among others, the straw is aerated so that the moisture content is 40%, the straw is stacked with a length of 2.5 m, a width of 2.5 m and a thickness of 25 cm, on top of the straw layer watered with water that has been mixed with urea until evenly distributed, on top of the straw layer sprinkled with starter until evenly distributed, the straw is stacked again with a thickness of 25 cm trampled until solid, repeated watering of water mixed with urea until evenly distributed, repeated sowing of starter until evenly distributed, so repeated until the pile can reach 3 m, after completion the top is covered with dry leaves such as banana leaves, rice straw is left for at least 3-4 weeks and fermented rice straw is ready to be given to livestock. The results of the service that have been achieved are the availability of alternative feed from fermented straw and can be given to cattle, where the cattle also want to consume it.
Perbedaan Viskositas Mucous Serviks dan Tipologi Ferning pada Sapi Aceh Repeat Breeding dan Fertil Cut Nila Thasmi; Herrialfian Herrialfian; Husnurrizal Husnurrizal; Hafizuddin Hafizuddin; Amalia Sutriana; Dasrul Dasrul; Marcelus Ferdinandez Titit; Muhammad Reza
Jurnal Agripet Vol 24, No 1 (2024): Volume 24, No. 1, April 2024
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v24i1.34687

Abstract

ABSTRAK. Efisiensi reproduksi ternak sangat ditentukan berdasarkan deteksi estrus. Terdapat beberapa indikator estrus yang dapat dijadikan patokan deteksi estrus diantaranya keluarnya mucous serviks. Mucous serviks akan diamati sifat fisiknya diantaranya warna, (tampilan), konsistensi (viskositas), elastisitas, tipologi ferning dan pH. Pengamatan terhadap nilai viskositas dan tipologi ferning mucous serviks dibagi menjadi dua faktor utama dalam penelitian ini. Penelitian ini bertujuan untuk mengetahui perbedaan viskositas mucous serviks dan tipologi ferning pada sapi Aceh repeat breeding dan sapi Aceh fertil. Dalam penelitian ini digunakan 10 ekor sapi Aceh, terdiri dari 6 ekor sapi fertil dan 4 ekor sapi repeat breeding berumur 3-5 tahun yang telah disinkronisasi dengan prostaglandin F2 alpha (PGF2) sebanyak 5 mL secara intra muskulus dengan pola penyuntikan tunggal. Penentuan viskositas mucous serviks diukur berdasarkan nilai spinnbarkeit yang dideteksi pada saat puncak estrus. Untuk menentukan tipology ferning, mucous serviks dioleskan di atas objek glass, lalu dikeringkan selanjutnya diamati di bawah mikroskop. Tipologi ferning diklasifikasikan berdasarkan kriteria tipikal, atipikal, dan nihil. Data yang diperoleh dianalisis secara deskriptif dan uji T. Hasil penelitian menunjukkan terdapat tidak ada perbedaan viskositas mucous serviks, namun terlihat adanya perbedaan tipology ferning antara sapi Aceh repeat breeding dan fertil.(Differences in cervical mucous viscosity and ferning typology in repeat breeding and fertile Aceh cows)ABSTRACT. Reproductive efficiency of livestock is determined based on estrus detection. There are several indicators of estrus that can be used as a benchmark for estrous detection, including cervical mucous discharge. Cervical mucus will be observed for its physical properties including color, (appearance), consistency (viscosity), elasticity, ferning and pH. Observation of the viscosity value and typology of cervical ferning mucous was divided into two main factors in this study. The purpose of the study was to identify the variations in mucous cervical ferning between fertile Aceh cattle and repeat breeding. In this study, 10 Aceh cattle were used, consisting of 6 fertile cattle and 4 repeat breeding aged 3-5 years which had been synchronized with 5 mL of prostaglandin F2 alpha (PGF2) intramuscularly with a single injection pattern. The determination of cervical mucous viscosity is measured based on the spinnbarkeit value detected at the peak of estrus. To determine the ferning typology, cervical mucous was applied to the glass object, allowed to dry, and then examined under a microscope. Ferning typology was classified based on the criteria of typical, atypical, and nil. The data obtained were analyzed descriptively and T-test. The results showed that there was no difference in cervical mucous viscosity, but there was a difference in ferning typology between repeat breeding and fertile Aceh cows.
The Effectiveness of Lugol on the Increasing of Pregnancy Rate in Aceh Cow with Endometritis Amalia Sutriana; Arman Sayuti; Budianto Panjaitan; Teuku Armansyah TR; Aisyah Fadillah Tunnisa; Juli Melia; Tongku Nizwan Siregar; Hafizuddin Hafizuddin; Dwinna Aliza
Jurnal Agripet Vol 21, No 2 (2021): Volume 21, No. 2, Oktober 2021
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v21i2.18513

Abstract

ABSTRACT. The objective of this study was to determine the effect of lugol on the increasing the pregnancy rate in repeat breeding (RB) Aceh cows due to endometritis. This study used six endometritiss cows, aged 5-7 years, weighed 150-250 kg which were divided into two groups (n=3), KI and KII. The cows in group 1 (K1) were injected with 5 ml PGF2, while the cows in group 2 (KII) were treated with 50 ml of 2% lugol intra-uterine and continued with an injection of 5 ml PGF2 after healing. The detection of estrus was performed twice a day following by artificial insemination (AI) about 10-16 hours after the onset of estrus. Determination of pregnancy was performed by ultrasonography (USG) on the 25th day after AI. The data obtained were analyzed descriptively. The results showed that all endometritis cows in KI and KII present estrous signs (100%). However, only one cow was recovered in K2, whereas in K1 did not. After AI, one pregnant cow was observed in KII (33.3%), while none of the pregnant cows was found in K1 (0.0%). It is concluded that the lugol treatment for endometritiss Aceh cows can improve the pregnancy rate.(Efektivitas larutan lugol untuk meningkatkan persentase kebuntingan pada sapi Aceh yang mengalami endometritis) ABSTRAK. Penelitian ini bertujuan mengetahui pengaruh pemberian larutan lugol terhadap peningkatan persentase kebuntingan sapi Aceh yang mengalami RB. Dalam penelitian ini digunakan enam ekor sapi Aceh betina dewasa, umur 5-7 tahun, bobot badan 150-250 kg, sudah pernah beranak, dan didiagnosis mengalami endometritis. Seluruh sapi dibagi menjadi dua kelompok (n=3). Pada kelompok 1 (K1), sapi endometritis diterapi dengan 50 ml lugol 2% secara intra uteri dan setelah sembuh dilanjutkan dengan penyuntikan 5 ml PGF2. Sapi pada kelompok 2 (K2) hanya diinjeksi dengan 5 ml PGF2. Deteksi berahi dilakukan sebanyak dua kali per hari dan inseminasi buatan (IB) dilakukan sekitar 10-16 jam setelah awal berahi. Pemeriksaan kebuntingan dilakukan dengan ultrasonografi (USG) pada hari ke-25 setelah IB. Data dianalisis secara deskriptif. Dari masing-masing kelompok, hanya satu ekor sapi pada K2 yang dinyatakan sembuh yaitu sapi pada kelompok kedua. Persentase sapi yang menjadi estrus pada kedua kelompok masing-masing adalah 100%. Dari tiga ekor sapi yang diinseminasi pada masing-masing kelompok, hanya satu ekor sapi pada K2 (33,3%) yang menunjukkan hasil positif bunting sedangkan pada K1 tidak terdapat sapi yang menunjukkan hasil positif (0,0%). Disimpulkan bahwa pemberian larutan lugol pada sapi Aceh yang mengalami endometritis dapat meningkatkan persentase kebuntingan sapi Aceh.
Potential of ant nest extract (Hydnophytum formicarum) for protection of testicular morphometry, epididymal functions, and sperm quality in male rats with alloxan-induced diabetes Roslizawaty, Roslizawaty; Abrar, Mahdi; Khairan, Khairan; Gholib, Gholib; Siregar, Tongku N.; Syafruddin, Syafruddin; Sutriana, Amalia; Azzahra, Putri; Aini, Hanipah
Narra J Vol. 4 No. 3 (2024): December 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i3.922

Abstract

Medicinal herbs, such as the ant nest plant (Hydnophytum formicarum), are promising for the management of diabetes mellitus-associated infertility. The aim of this study was to evaluate the biological activity of the ant nest plant and its capacity to mitigate the adverse effects of alloxan-induced diabetes on testicular morphology, epididymal function, and sperm quality in male rats. The tuber of the ant nest plant was extracted using methanol and then subjected to phytochemical screenings. For the experiment, 20 male white rats (Rattus norvegicus), aged 3–4 months and weighing 150–200 g, were equally divided into four groups. The ant nest extract was administered orally using oral gavage over 14 days. The testes, epididymis, and sperm were collected for weighing, morphometric measurements, and quality evaluation. Qualitative testing of phytochemical compounds indicated the presence of flavonoids, tannins, steroids, terpenoids, and phenolic compounds in the plants. The results revealed the protective effects of ant nest extract against the adverse fertility effects induced by alloxan and a high-fat diet, as observed in testicular weight (p=0.045), epididymal weight (p=0.041), and sperm quality (p>0.05).
Hormonal induction of gonad maturation in female tinfoil barb fish (Barbonymus schwanenfeldii) using spawnprim hormone Amalia Sutriana; Ahmad Baihaqi; Iwan Hasri; Nurliana; Hafizuddin Hafizuddin
Jurnal Akuakultur Indonesia Vol. 21 No. 2 (2022): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.21.2.118-124

Abstract

The aim of this study was to analyze the effect of spawnprim hormone induction with different doses on gonadal maturity of tinfoil barb fish broodstock (Barbonymus schwanenfeldii). A completely randomized design (CRD) was used in this study with 4 treatments and 3 replications. Fish broodstock in group A (control) was not given any treatment, while fish broodstocks in groups B, C, and D were injected with spawnprim hormone at doses of 0.3 ml/kg body weight, 0.6 ml/kg body weight, and 0.9 ml/kg body weight, respectively. The measured parameters were gonad maturity level, broodstock weight gain, egg diameter, and fecundity. Data were analyzed using one way analysis of variance (ANOVA). The result showed that the gonad maturity level (GML) in groups B, C, and D (GML III and IV) was better than group A (GML I). Statistical analysis showed that the induction of spawnprim hormone on tinfoil barb broodstocks significantly affect (P<0.05) broodstock weight gain, egg diameter, and fecundity. The optimal dose of spawnprim hormone for tinfoil barb was 0.6 ml/kg body weight, with the average broodstock weight gain, egg diameter, and fecundity were 0.011 kg, 1.55 mm, and 102.15 eggs, respectively. The induction of spawnprim hormone has a positive effect on gonad maturation of tinfoil barb fish (B. schwanenfeldii), with the optimum dose is 0.6 ml/kg fish Keywords: gonad maturity, tinfoil barb fish, spawnprim ABSTRAK Penelitian ini bertujuan untuk menganalisis pengaruh penggunaan hormon spawnprim dengan dosis yang berbeda terhadap kematangan gonad induk ikan lemeduk Barbonymus schwanenfeldii. Metode yang digunakan pada penelitian ini adalah metode rancangan acak lengkap (RAL) dengan 4 kelompok perlakuan dan 3 kali pengulangan. Ikan pada kelompok A (kontrol) tidak diberikan perlakuan, sedangkan ikan pada kelompok B, C, dan D masing-masing diinjeksi hormon spawnprim dengan dosis 0.3 ml/kg bobot badan; 0.6 ml/kg bobot badan; dan 0,9 ml/kg bobot badan. Parameter yang diamati adalah persentase induk matang gonad akhir, pertambahan bobot induk, pertambahan diameter telur, dan fekunditas. Data dianalisis dengan menggunakan analisis varians (ANAVA) pola satu arah. Hasil penelitian menunjukkan tingkat kematangan gonad (TKG) pada kelompok perlakuan B, C, dan D (TKG III dan IV) lebih baik dari kelompok A (TKG I). Hasil uji ANAVA menunjukkan bahwa pemberian hormon spawnprim pada induk ikan lemeduk berpengaruh nyata (P<0.05) terhadap pertambahan bobot induk, pertambahan diameter telur, dan fekunditas. Tingkat kematangan gonad terbaik pada penelitian ini diperoleh pada kelompok perlakuan C yang diinduksi hormon spawnprim dengan dosis 0.6 ml/kg bobot badan induk ikan dengan rata - rata pertambahan bobot induk, pertambahan diameter telur, dan fekunditas masing-masing adalah 0.011 kg, 1.55 mm, dan 102.15 butir telur. Induksi hormon spawnprim menimbulkan pengaruh yang positif terhadap pertambahan bobot induk, diameter telur dan fekunditas ikan lemeduk (B. schwanenfeldii), dengan dosis optimum 0.6 ml/kg bobot badan. Kata kunci: kematangan gonad, ikan lemeduk, spawnprim
Co-Authors Abdul Harris Abdullah A. Muhammadar Ahmad Baihaqi Ahsan, Muhammad Maulana Aini, Hanipah Aisyah Fadillah Tunnisa Ajirni Ajirni Amiruddin Amiruddin Annisa Anwar Sitorus Anwar Anwar Arman Sayuti Arman Sayuti Arman Sayuti Asmawati Asmawati Azzahra, Putri Batubara, Agung Setia Budianto Panjaitan Budianto Panjaitan Budianto Panjaitan Budianto Panjaitan Budianto Panjaitan Cut Nila Thasmi Cut Sriyanti D Dasrul Darmawi Darmawi Dasrul Dasrul Dasrul Dasrul Dedi Fazriansyah Putra Dian Masyitha Dwina Aliza Dwinna Aliza Dwinna Aliza Dwinna Aliza Elza Savitri Erdiansyah Rahmi Erdiansyah Rahmi Erdiansyah Rahmi Erina Erina Erina Erina Fakhrurrazi Fakhrurrazi Fakhrurrazi Fakhrurrazi Farid Wajidi Feby Fema Amzani Nasution Firdus Firdus Fuza Khoiriah Gani, Azhari Gholib Gholib, Gholib Ginta Riady Hafizuddin Hafizuddin Hafizuddin Hafizuddin Hamdan Hamdan Hasan, Denny I. Henni Vanda HENNIVANDA HENNIVANDA Herrialfian . Herrialfian Herrialfian Husnurrizal . Husnurrizal Husnurrizal Husnurrizal, Husnurrizal Ismail Ismail Iwan Hasri Juli Melia Juli Melia KARTINI ERIANI Karunita Dewi Khairan Khairan Mada Admi Mahdi Abrar Marcelus Ferdinandez Titit Masda Admi Mentari Azhari L Mira Ayu Lestari Hasibuan Mst Nahid Akter Muchlisin Zainal Abidin Mudatsir Mudatsir Muhammad Ammar Muhammad Hambal Muhammad Hambal Muhammad Hanafiah Muhammad Hanif Muhammad Isa Muhammad Reza Mulyadi Adam Nada Sarah Syahputri Nazaruddin Nazaruddin Nazaruddin Nazaruddin Novianti, Arindita Niatazya Nurhazimah Nurhazimah Nurliana NURLIANA NURLIANA Nuzul Asmilia Nuzul Asmilia Nuzul Asmilia R Roslizawaty Rasmaidar . Raysa Syarifah Razali Daud Rinidar Rinidar Roshada Hashim Roslizawaty Roslizawaty Roslizawaty Roslizawaty Roslizawaty Roslizawaty, Roslizawaty Rosmaidar Rosmaidar Rossy Septia Putri S Syafruddin Sembiring, Rinawati Siregar, Tongku N. Siti Aisyah Siti Azizah Mohd Nor Sri Wahyuni Sri Wahyuni Sudi Indriany Sugito Sugito Syafruddin Syafruddin Syafruddin Syafruddin Syafruddin Syafruddin T Armansyah TR T. Armansyah T. Armansyah T. Armansyah Teuku Armansyah Teuku Armansyah Tongku N. Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar, MP Triva Murtina Lubis Winaruddin Winaruddin Yayang Nuri Al Aliya Zakwan, Muhammad