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Hormonal induction of gonad maturation in female tinfoil barb fish (Barbonymus schwanenfeldii) using spawnprim hormone Amalia Sutriana; Ahmad Baihaqi; Iwan Hasri; Nurliana; Hafizuddin Hafizuddin
Jurnal Akuakultur Indonesia Vol. 21 No. 2 (2022): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.21.2.118-124

Abstract

The aim of this study was to analyze the effect of spawnprim hormone induction with different doses on gonadal maturity of tinfoil barb fish broodstock (Barbonymus schwanenfeldii). A completely randomized design (CRD) was used in this study with 4 treatments and 3 replications. Fish broodstock in group A (control) was not given any treatment, while fish broodstocks in groups B, C, and D were injected with spawnprim hormone at doses of 0.3 ml/kg body weight, 0.6 ml/kg body weight, and 0.9 ml/kg body weight, respectively. The measured parameters were gonad maturity level, broodstock weight gain, egg diameter, and fecundity. Data were analyzed using one way analysis of variance (ANOVA). The result showed that the gonad maturity level (GML) in groups B, C, and D (GML III and IV) was better than group A (GML I). Statistical analysis showed that the induction of spawnprim hormone on tinfoil barb broodstocks significantly affect (P<0.05) broodstock weight gain, egg diameter, and fecundity. The optimal dose of spawnprim hormone for tinfoil barb was 0.6 ml/kg body weight, with the average broodstock weight gain, egg diameter, and fecundity were 0.011 kg, 1.55 mm, and 102.15 eggs, respectively. The induction of spawnprim hormone has a positive effect on gonad maturation of tinfoil barb fish (B. schwanenfeldii), with the optimum dose is 0.6 ml/kg fish Keywords: gonad maturity, tinfoil barb fish, spawnprim ABSTRAK Penelitian ini bertujuan untuk menganalisis pengaruh penggunaan hormon spawnprim dengan dosis yang berbeda terhadap kematangan gonad induk ikan lemeduk Barbonymus schwanenfeldii. Metode yang digunakan pada penelitian ini adalah metode rancangan acak lengkap (RAL) dengan 4 kelompok perlakuan dan 3 kali pengulangan. Ikan pada kelompok A (kontrol) tidak diberikan perlakuan, sedangkan ikan pada kelompok B, C, dan D masing-masing diinjeksi hormon spawnprim dengan dosis 0.3 ml/kg bobot badan; 0.6 ml/kg bobot badan; dan 0,9 ml/kg bobot badan. Parameter yang diamati adalah persentase induk matang gonad akhir, pertambahan bobot induk, pertambahan diameter telur, dan fekunditas. Data dianalisis dengan menggunakan analisis varians (ANAVA) pola satu arah. Hasil penelitian menunjukkan tingkat kematangan gonad (TKG) pada kelompok perlakuan B, C, dan D (TKG III dan IV) lebih baik dari kelompok A (TKG I). Hasil uji ANAVA menunjukkan bahwa pemberian hormon spawnprim pada induk ikan lemeduk berpengaruh nyata (P<0.05) terhadap pertambahan bobot induk, pertambahan diameter telur, dan fekunditas. Tingkat kematangan gonad terbaik pada penelitian ini diperoleh pada kelompok perlakuan C yang diinduksi hormon spawnprim dengan dosis 0.6 ml/kg bobot badan induk ikan dengan rata - rata pertambahan bobot induk, pertambahan diameter telur, dan fekunditas masing-masing adalah 0.011 kg, 1.55 mm, dan 102.15 butir telur. Induksi hormon spawnprim menimbulkan pengaruh yang positif terhadap pertambahan bobot induk, diameter telur dan fekunditas ikan lemeduk (B. schwanenfeldii), dengan dosis optimum 0.6 ml/kg bobot badan. Kata kunci: kematangan gonad, ikan lemeduk, spawnprim
Aplikasi Metode Pemeriksaan Kimia Urin Untuk Diagnosis Kebuntingan Dini Pada Sapi Masyarakat Di Desa Lampuuk Kecamatan Kuta Baro Kabupaten Aceh Besar Syafruddin Syafruddin; Arman Sayuti; Tongku Nizwan Siregar, MP; Teuku Armansyah; Sri Wahyuni; Hafizuddin Hafizuddin; Budianto Panjaitan; Amalia Sutriana; Mulyadi Adam
Peternakan Abdi Masyarakat (PETAMAS) Vol 3, No 1 (2023): Volume 3, Nomor 1, Juni 2023
Publisher : Departemen of Animal Science, Agriculture Faculty, Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/petamas.v3i1.30921

Abstract

Kegiatan pengabdian kepada masyarakat bertujuan meningkatkan pengetahuan peternak tentang kerugian yang diakibatkan oleh ketiadaan pemeriksaan kebuntingan dan meningkatkan pengetahuan dan keterampilan inseminator tentang metode pemeriksaan kimia urin. Kelompok sasaran dari kegiatan ini adalah 15 (lima belas) orang masyarakat petani peternak sapi di Desa Lampuuk Kecamatan Kuta Baro Kabupaten Aceh Besar dan 2 (dua) orang inseminator di wilayah setempat. Pelaksanaan kegiatan ini dilakukan dengan 2 metode, yaitu (1) metode pendidikan dan penyuluhan dan (2) demonstrasi dan pelatihan di lapangan. Pada kegiatan penyuluhan, peternak akan diajarkan tentang kerugian akibat ketiadaan diagnosis kebuntingan. Kepada inseminator setempat diperkenalkan cara dan alat/bahan yang diperlukan untuk diagnosis kebuntingan. Pada kegiatan demonstrasi akan dilakukan pemeriksaan urin sapi yang telah diinseminasi buatan. Dari hasil kegiatan disimpulkan bahwa pengetahuan peternak tentang kerugian yang diakibatkan oleh ketiadaan pemeriksaan kebuntingan meningkat dan inseminator terampil melakukan diagnosis kebuntingan melalui pemeriksaan kimia urin.
Total Plate Numbers and Coliforms in The Processing of Milkfish Without Spines (Chanos Chanos) in Household Indusrty in Banda Aceh Nurliana, Nurliana; Aftari, Nailul; Sutriana, Amalia; Asmilia, Nuzul; Sari, Wahyu Eka
Jurnal Medika Veterinaria Vol 17, No 2 (2023): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v17i2.34843

Abstract

Microbial contamination of fish has the potential to cause product quality degradation and cause fishborne disease. Microbial contamination can occur at any stage of fish processing. This study aimed to determine the total plate count and coliform at each stage process of milkfish (Chanos chanos) boneless. Milkfishes were obtained from three processing stage, namely raw material (fresh fish) stage, the fish bone removal stage and the baking fish stage. The parameters observed were the number of colonies growing as total plate count and coliform as the most probable number (MPN). The results showed that the highest ALT contamination was found at the bone removal stage with an average of 1.7x104 CFU/g but this figure did not exceed the standard limit and the lowest was at the firing stage with an average of 1.2x103 CFU/g. The highest coliform was found in the fresh fish stage, namely 129 MPN/g and the lowest was 3 MPN/g. From this research, it can be concluded that the total microbial plate number does not exceed the SNI 7288: 2009 standard and the coliform exceed the SNI 7388: 2009 standard.
Determination of estrus in Aceh cows based on the van Eerdenburg method Qathrinnada Ramadhana; Ginta Riady; Hafizuddin Hafizuddin; Cut Nila Thasmi; Erdiansyah Rahmi; Amalia Sutriana
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 3 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i3.2022.103-108

Abstract

Accurate determination of estrus status in an animal based on an appropriate estrus detection method can guarantee the cows' pregnancy after mating. This study aims to determine the estrus status of Aceh cows using the van Eerdenburg estrus behavior scoring method. This study was conducted from 22 January to 12 February 2021, at the Technical Implementation Unit for Animals of the Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Indonesia. The sample used was eleven Aceh cows aged 4-6 years old. Parameters observed were the number of cows exhibiting signs of estrus using the estrus behavior scoring method. The accuracy of estrus detection was confirmed by palpation per rectum to detect the presence of uterine tone. By observing signs of estrus using the method studied, 90.9% (10/11) of the cows were determined to be in estrus. Confirmation by the presence of uterine tone showed a 100% accuracy rate. The most effective sign of estrus was the passivity of the cow when being ridden by another cow or bull. This study concluded that estrus status in local cows could be determined using the estrus behavior scoring of the van Eerdenburg method.
THE EFFECT OF WHOLE SEED (Barringtonia racemosa) WATER EXTRACT ON ERYTHROCYTE, HEMOGLOBIN AND HEMATOCRITE COUNT OF WHITE RAT (Rattus norvegicus) EXPOSED TO CIGARETTE SMOKE Dasrul, D; Aliya, Yayang Nuri Al; Sutriana, Amalia; Asmilia, Nuzul; Daud, Razali
Jurnal Kedokteran Hewan Vol 17, No 2 (2023): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i2.25424

Abstract

This study aims to determine the effect of Barringtonia racemosa seed extract on the number of erythrocytes, hemoglobin levels, and the percentage of hematocrit in white rats (Rattus norvegicus) exposed to cigarette smoke. A total of 25 white rats Wistar strain, aged 3-4 months, male, weighing 180-200 g, were randomly divided into 5 treatment groups, each group consisting of 5 rats. Positive control group (KP) without exposure to cigarette smoke and without administration of Barringtonia racemosa seed extract, negative control group (KN) exposed to cigarette smoke without administration of Barringtonia racemosa seed extract. Treatment groups P1, P2, P3 were exposed to cigarette smoke and given Barringtonia racemosa seeds with doses of 50, 100, and 150 mg/kg BW/day, respectively. The extract was administered orally for 30 days. Blood collection in all groups was carried out via the orbital vein using a hematocrit pipette after the rats had been sedated with 0.2 mL of ketamine. Erythrocyte, hemoglobin, and hematocrit values were calculated using a hematology analyzer. Data were analyzed using one-way pattern analysis of variance (ANOVA). The results showed that giving Barringtonia racemosa seeds at doses of 50, 100 and 150 mg/kg/day had a significant effect (P0.05) in increasing the values of erythrocytes, hemoglobin, and hematocrit of white rats exposed to cigarette smoke compared to the control group which was not given the extract. In conclusion, the administration of Barringtonia racemosa seed extract can increase the number of erythrocytes, hemoglobin levels, and the percentage of hematocrit exposed to cigarette smoke.
EFFECT OF SALINITY CHANGES ON METHIONINE CONTENT IN TIGER GROUPER JUVENILE (Epinephelus fuscoguttatus) Abbas, Muhammadar Abdullah; Abidin, Muchlisin Zainal; Putra, Dedi Fazriansyah; Firdus, Firdus; Batubara, Agung Setia; Sail, Asmawati Muhammad; Syafruddin, Syafruddin; Roslizawaty, Roslizawaty; Sutriana, Amalia; Aliza, Dwinna
Jurnal Kedokteran Hewan Vol 12, No 4 (2018): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i4.12363

Abstract

This study was conducted to determine the effect of water salinity changes on methionine content in tiger grouper juvenile (Epinephelus fuscoguttatus). A total of 2,560 tiger grouper juveniles were used and divided into five groups consist of 1 control group (without exposed to salinity changes) and 4 treatment groups. The salinity was changed every 2, 3, 4, and 6 hours in treatment A, B, C, and D, respectively. Salinity levels were changed during 24 hours by lowering salinity level from 32 psu to 22 psu. Twenty five of fish were collected from each treatment for methionine content analysis. Data were analysed using one way analysis of variance (ANOVA). The results showed that there was a decrease in methionine content in all treatments. A significant decrease (P0.05) of methionine content in treatment A, B, and C were observed after 20 hours (1.15%), 18 hours (1.27%), and 16 hours (1.24%), respectively. While at 0 hours (control), the methionine content was 2.02%. Methionine level in treatment D was not significantly different (P0.05) compared to control group. As conclusion, rearing the tiger grouper juvenile with salinity fluctuations every 6 hours did not lead to methionine deficiency.
EFFECTIVENESS OF THE FERMENTATIVE EXTRACT OF Lactobacillus acidophilus AS ANTIMICROBIALS AGAINST Aeromonas hydrophila Akter, Mst Nahid; Hashim, Roshada; Sutriana, Amalia; Mohd Nor, Siti Azizah
Jurnal Kedokteran Hewan Vol 12, No 4 (2018): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i4.11920

Abstract

The purpose of this study was to evaluate the in vitro and in vivo antimicrobial activity of the commercial Lactobacillus acidophilus (L. acidophilus) cells and cell free extract against Aeromonas hydrophila (A. hydrophila). The in vitro method was carried out using well diffusion method. For in vivo evaluation, the effect of L. acidophilus on the survival rate of Pangasianodon hypophthalmus (P. hypophthalmus) infected with A. hydrophila was evaluated. The well diffusion method showed a significant inhibition ability of L. acidophilus cells against A. hydrophila compared to the cell free extract. The inhibition diameters obtained with cells and cell free extract were 17.23 mm and 15.17 mm, respectively. P. hypophthalmus injected with L. acidophilus cells and cell free extract following challenged with A. hydrophila cells showed survival rate of 70% and 60% respectively, at 2-week post challenged. The gas chromatography-mass spectrophotometry (GC-MS) result revealed that a diverse of compounds was detected in both the L. acidophilus cells and cell free extract, among them the most abundant component was pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl), which showed a promising anticancerous activity and might be played a significant role in the recovery of the infectious P. hypophthalmus. The current study revealed that both cells and cell free extract of L. acidophilus have antimicrobial activity against A. hydrophila.
Effect of Storage Time of Epididymis at 5 oC on Spermatozoa Quality of Aceh Local Goat Hamdan, Hamdan; Panjaitan, Budianto; Sutriana, Amalia; Aliza, Dwinna; Rahmi, Erdiansyah
Jurnal Kedokteran Hewan Vol 4, No 2 (2010): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i2.9811

Abstract

The objective of this study was to evaluate the effect of storage time of epididymis at 5 oC on spermatozoa quality of Aceh local goat. This research was conducted at Reproduction Laboratory, FKH Unsyiah from May to June 2009. Nine epididymis from local goat at the age of 1.5-2.0 years were used in this study. The epididymis were collected from abattoir in Banda Aceh. The experimental design used in this study was completely randomized design with 3 treatments and 3 replications. Spermatozoa quality which consist of sperm concentration, motility, amount of life sperm, and sperm abnormality were examined after the collection of epididymis on day 0 (H-0), day 1 (H-1), and day 3 (H-3) post storage at 5 oC. Data were analyzed using one-way analyses of variance (ANOVA). The result showed that no significant difference (P0.05) seen in the spermatozoa quality after different storage time. The average of spermatozoa concentration on H-0, H-1, and H-3 were 318x107/ml, 282x107/ml, and 241x107/ml respectively. On the average, the percentage of spermatozoa motility on H-0, H-1, and H-3 were 82,272,75; 80,252,83; and 78,070,92%, respectively. Life spermatozoa observed on H-0, H-1, and H-3 were 82,292,71; 80,631,87; and 80,093,31%, respectively. Observation on spermatozoa abnormality showed that the average of spermatozoa abnormality on H-0, H-1, and H-3 were 7,230,27; 8,210,55; and 10,753,14%, respectively. It could be concluded that the spermatozoa quality were not affected by the storage time at temperature of 5 oC.
Characterization of Inhibin from Culture and Non Culture of Granulose Cells for Monoclonal Antibody of Inhibin Production Amiruddin, Amiruddin; Nizwan Siregar, Tongku; Sutriana, Amalia; Aliza, Dwinna; Armansyah, T.
Jurnal Kedokteran Hewan Vol 4, No 1 (2010): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i1.9789

Abstract

This study has long-term objectives to obtain immunogenic prototype that can be used to induce multiple ovulation in goats. Working steps of this study were begun with the collection of ovarium from goats, collection of granulose cells, culture of granulose and characterization of molecular weight and isoelectric point (pI) of inhibin protein of granulose cells obtained from culture and non-culture of granulose cells, and followed by preparation of monoclonal antibody toward inhibin. The results showed that inhibin isolated either from culture or non-culture of granulose cells produced a 32 kDa band. Molecular weight of inhibin was measured by Western Blot. The 32 kDa band of SDS PAGE product appeared on Western Blot result was inhibin molecules produced by granulose cells collected fom culture and non-culture of granulose cells that can be identified by Mab-inhibin. Product of IEF gel electrophoresis suggested that inhibin molecule collected from culture of granulose cells has no charge at isoelectric points ranging from 5-6, depends on its total amino acid composition.
UJI TOKSISITAS AKUT EKSTRAK ETANOL DAUN MALAKA (Phyllantus emblica) TERHADAP MENCIT (Mus musculus). (Acute Toxicity Test of Ethanolic Extract of Malaka (Phyllantus emblica) Leaves on Mice (Mus musculus)) TR, T. Armansyah; Indriany, Sudi; Sutriana, Amalia; Rosmaidar, Rosmaidar; Asmilia, Nuzul; Panjaitan, Budianto; Aliza, Dwinna; Hamdan, Hamdan
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5137

Abstract

ABSTRACT The aim of this research was to asses the acute toxicity of ethanolic extract of malaka leaves using lethal dose 50 (LD50) on mice (Mus musculus). Twenty male mice weighing between 20-30 g were randomly divided into 4 groups (group K1-K4) of 5 mice each. All mice in group K1, K2, K3, and K4 were administered ethanolic extract of malaka leaves with the dose of 2, 4, 8, and 16 g/kg bw, respectively. Single dose of ethanolic extract of malaka leaves were given by oral gavage prior to clinical observation . The observation period was 14 days post administration, for sign of toxicity symptom, weight loss, and mortality. The result showed that no mortality was observed in the experimental animals during this study. Slight reduction of body weight was observed in group K2, K3, and K4, and no toxicity sign was found during fourteen days of observation. The LD50 of ethanolic extract of malaka leaves was higher than 16 g/kg body weight, thus, the substance was practically non toxic substance.
Co-Authors Abbas, Muhammadar Abdullah Abdul Harris Abdullah A. Muhammadar Abidin, Muchlisin Zainal Aftari, Nailul Ahmad Baihaqi Ahsan, Muhammad Maulana Aini, Hanipah Aisyah Fadillah Tunnisa Ajirni Ajirni Akter, Mst Nahid Aliya, Yayang Nuri Al Amiruddin Amiruddin Amiruddin Amiruddin Annisa Anwar Sitorus Anwar Anwar Arman Sayuti Arman Sayuti Arman Sayuti Arman Sayuti Asmawati Asmawati Azzahra, Putri Batubara, Agung Setia Budianto Panjaitan Budianto Panjaitan Budianto Panjaitan budianto panjaitan Budianto Panjaitan Budianto Panjaitan Cut Nila Thasmi Cut Nila Thasmi, Cut Nila D Dasrul Darmawi Darmawi Dasrul Dasrul Dasrul, D Dedi Fazriansyah Putra Dian Masyitha Dwina Aliza Dwinna Aliza Dwinna Aliza Dwinna Aliza Elza Savitri Erdiansyah Rahmi Erdiansyah Rahmi Erdiansyah Rahmi Erina Erina Erina Erina Fakhrurrazi Fakhrurrazi Fakhrurrazi Fakhrurrazi Farid Wajidi Feby Fema Amzani Nasution Firdus Firdus Firdus Firdus Fuza Khoiriah Gani, Azhari Gholib Gholib, Gholib Ginta Riady Ginta Riady Hafizuddin Hafizuddin Hafizuddin Hafizuddin Hamdan Hamdan Hamdan Hamdan Hasan, Denny I. Henni Vanda HENNIVANDA HENNIVANDA Herrialfian . Herrialfian, Herrialfian Husnurrizal . Husnurrizal, Husnurrizal Indriany, Sudi Ismail Ismail Iwan Hasri Juli Melia Juli Melia KARTINI ERIANI Karunita Dewi Khairan Khairan Mada Admi Mahdi Abrar Masda Admi Mentari Azhari L Mira Ayu Lestari Hasibuan Mohd Nor, Siti Azizah Mst Nahid Akter Muchlisin Zainal Abidin Mudatsir Mudatsir Muhammad Ammar Muhammad Hambal Muhammad Hambal Muhammad Hanafiah Muhammad Hanif Muhammad Isa Muhammad Reza Mulyadi Adam Mulyadi Adam, Mulyadi Nada Sarah Syahputri Nazaruddin Nazaruddin Nazaruddin Nazaruddin Novianti, Arindita Niatazya Nurhazimah Nurhazimah Nurliana NURLIANA NURLIANA Nuzul Asmilia Nuzul Asmilia Nuzul Asmilia Putra, Dedi Fazriansyah Qathrinnada Ramadhana R Roslizawaty R Roslizawaty, R Rasmaidar . Raysa Syarifah Razali Daud Razali Daud Rinidar Rinidar Roshada Hashim Roshada Hashim Roslizawaty Roslizawaty Roslizawaty Roslizawaty Roslizawaty Roslizawaty, Roslizawaty Rosmaidar Rosmaidar Rosmaidar Rosmaidar, Rosmaidar Rossy Septia Putri S Syafruddin Sail, Asmawati Muhammad Sari, Wahyu Eka Sembiring, Rinawati Siregar, Tongku N. Siti Aisyah Siti Azizah Mohd Nor Sri Wahyuni Sri Wahyuni Sriyanti, Cut Sudi Indriany Sugito Sugito Syafruddin Syafruddin Syafruddin Syafruddin Syafruddin Syafruddin Syafruddin, S T Armansyah TR T. Armansyah T. Armansyah T. Armansyah Teuku Armansyah Teuku Armansyah Titit, Marcelus Ferdinandez Tongku N. Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar, MP Triva Murtina Lubis Winaruddin Winaruddin Yayang Nuri Al Aliya Zakwan, Muhammad