Claim Missing Document
Check
Articles

Aktivitas Hepatoprotektif Ekstrak Etanol Daun Kucing-kucingan Acalypha indica L.) pada Tikus Putih (Rattus Novergicus) yang Diinduksi Parasetamol T Armansyah TR; Amalia Sutriana; Dwinna Aliza; Henni Vanda; erdiansyah rahmi
Jurnal Ilmiah Ilmu-Ilmu Peternakan Vol. 13 No. 6 (2010): Mei 2010
Publisher : Fakultas Peternakan Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (56.896 KB) | DOI: 10.22437/jiiip.v0i0.120

Abstract

The  research  has  been  conducted  to  determine  hepatoprotective  effect  of  ethanolic  extract  of Acalypha  indica  L.  leaves  on  rats  (Rattus  novergicus)  induced  with  paracetamol.  The  study  was performed  following  a  completely  randomized  design, using  45 male  rats  (Rattus  novergicus)  divided into  5  groups.  Rats  in  group  1  (K1)  were  given  CMC  1  %  for  7  days  and  followed  by  aquadest administration 8 hours post given CMC on day 7. Group 2 was given CMC 1 % for 7 days and treated with paracetamol at dose 2,5 g/kg BW 8 hours post given CMC on day 7. Groups 3 (K3), 4 (K4), and 5 (K5) were pretreated with ethanolic extract of Acalypha indica L.  leaves at the dose of 50, 100, and 200 mg/kg BW for 7 days respectively, followed by paracetamol administration at the dose of 2.5 g/kg BW in the  next  8  hours  on  day  7. Paracetamol  hepatotoxicity  and  extracts  activity were measured  based  on SGPT and SGOT activity level on day 0, 8 hours after  last administration of  extract on day 7, and 24 hours post paracetamol administration. Data were analyzed using one way ANOVA. The result showed that  the  administration  of  ethanolic  extract  of Acalypha  indica  leaves  at  the  dose  of  50,  100,  and  200 mg/kg BW was  significantly  reduced SGPT  and SGOT  level  (P<0,05)  in  rats  that were  induced with paracetamol. Based on the results of SGPT and SGOT serum activity, it can be concluded that ethanolic extract of Acalypha indica L. potential as hepatoprotective agent.
Effect of Storage Time of Epididymis at 5 oC on Spermatozoa Quality of Aceh Local Goat Hamdan Hamdan; Budianto Panjaitan; Amalia Sutriana; Dwinna Aliza; Erdiansyah Rahmi
Jurnal Kedokteran Hewan Vol 4, No 2 (2010): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.236 KB) | DOI: 10.21157/j.ked.hewan.v4i2.9811

Abstract

The objective of this study was to evaluate the effect of storage time of epididymis at 5 oC on spermatozoa quality of Aceh local goat. This research was conducted at Reproduction Laboratory, FKH Unsyiah from May to June 2009. Nine epididymis from local goat at the age of 1.5-2.0 years were used in this study. The epididymis were collected from abattoir in Banda Aceh. The experimental design used in this study was completely randomized design with 3 treatments and 3 replications. Spermatozoa quality which consist of sperm concentration, motility, amount of life sperm, and sperm abnormality were examined after the collection of epididymis on day 0 (H-0), day 1 (H-1), and day 3 (H-3) post storage at 5 oC. Data were analyzed using one-way analyses of variance (ANOVA). The result showed that no significant difference (P0.05) seen in the spermatozoa quality after different storage time. The average of spermatozoa concentration on H-0, H-1, and H-3 were 318x107/ml, 282x107/ml, and 241x107/ml respectively. On the average, the percentage of spermatozoa motility on H-0, H-1, and H-3 were 82,27±2,75; 80,25±2,83; and 78,07±0,92%, respectively. Life spermatozoa observed on H-0, H-1, and H-3 were 82,29±2,71; 80,63±1,87; and 80,09±3,31%, respectively. Observation on spermatozoa abnormality showed that the average of spermatozoa abnormality on H-0, H-1, and H-3 were 7,23±0,27; 8,21±0,55; and 10,75±3,14%, respectively. It could be concluded that the spermatozoa quality were not affected by the storage time at temperature of 5 oC.
THE USE OF CASSAVA LEAVES AS A DIETARY COMPONENT FOR AFRICAN CATFISH FRY Amalia Sutriana
Jurnal Kedokteran Hewan Vol 1, No 2 (2007): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (127.353 KB) | DOI: 10.21157/j.ked.hewan.v1i2.3128

Abstract

 An experiment was carried out to evaluate the effect of graded levels of cassava leaf meal (CLM) with varying cyanide content on growth performance of African catfish (Clariasgariepinus) fry. There were four groups of fish, each comprising 12 fish per group. One groupwas fed on a control diet (0 mg HCN/kg diet) with fish meal (FM) and soybean meal (SBM) asprotein source (30% CP). Groups two, three and four were fed graded levels of CLM to replace10, 20, and 30% of the total diet at the expense of SBM which contains 4.19, 7.47 and 11.96 mgHCN/ kg diet respectively. After 12 weeks, fish fed with increasing level of CLM showed asignificant growth depression and poor feed utilization compared to those fed the control diet.Apparent dry matter digestibility (ADMD) and apparent protein digestibility (APD) were alsosignificantly (P0.05) affected by cassava leaf meal inclusion, with the best values were found infish fed the control diet (52.26+0.24% and 83.11+1.17% for ADMD and APD respectively). Bodycomposition of fish fed higher CLM tended to have less protein and fat but more ash. The poormethionine availability as a result of low protein digestibility and the presence cyanide contentlimits the use of CLM in African catfish fry diet.Keywords: African catfish, cassava, growth, cyanide
EFFECT OF SALINITY CHANGES ON METHIONINE CONTENT IN TIGER GROUPER JUVENILE (Epinephelus fuscoguttatus) Muhammadar Abdullah Abbas; Muchlisin Zainal Abidin; Dedi Fazriansyah Putra; Firdus Firdus; Agung Setia Batubara; Asmawati Muhammad Sail; Syafruddin Syafruddin; Roslizawaty Roslizawaty; Amalia Sutriana; Dwinna Aliza
Jurnal Kedokteran Hewan Vol 12, No 4 (2018): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.791 KB) | DOI: 10.21157/j.ked.hewan.v12i4.12363

Abstract

This study was conducted to determine the effect of water salinity changes on methionine content in tiger grouper juvenile (Epinephelus fuscoguttatus). A total of 2,560 tiger grouper juveniles were used and divided into five groups consist of 1 control group (without exposed to salinity changes) and 4 treatment groups. The salinity was changed every 2, 3, 4, and 6 hours in treatment A, B, C, and D, respectively. Salinity levels were changed during 24 hours by lowering salinity level from 32 psu to 22 psu. Twenty five of fish were collected from each treatment for methionine content analysis. Data were analysed using one way analysis of variance (ANOVA). The results showed that there was a decrease in methionine content in all treatments. A significant decrease (P0.05) of methionine content in treatment A, B, and C were observed after 20 hours (1.15%), 18 hours (1.27%), and 16 hours (1.24%), respectively. While at 0 hours (control), the methionine content was 2.02%. Methionine level in treatment D was not significantly different (P0.05) compared to control group. As conclusion, rearing the tiger grouper juvenile with salinity fluctuations every 6 hours did not lead to methionine deficiency.
EFFECTIVENESS OF THE FERMENTATIVE EXTRACT OF Lactobacillus acidophilus AS ANTIMICROBIALS AGAINST Aeromonas hydrophila Mst Nahid Akter; Roshada Hashim; Amalia Sutriana; Siti Azizah Mohd Nor
Jurnal Kedokteran Hewan Vol 12, No 4 (2018): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (880.865 KB) | DOI: 10.21157/j.ked.hewan.v12i4.11920

Abstract

The purpose of this study was to evaluate the in vitro and in vivo antimicrobial activity of the commercial Lactobacillus acidophilus (L. acidophilus) cells and cell free extract against Aeromonas hydrophila (A. hydrophila). The in vitro method was carried out using well diffusion method. For in vivo evaluation, the effect of L. acidophilus on the survival rate of Pangasianodon hypophthalmus (P. hypophthalmus) infected with A. hydrophila was evaluated. The well diffusion method showed a significant inhibition ability of L. acidophilus cells against A. hydrophila compared to the cell free extract. The inhibition diameters obtained with cells and cell free extract were 17.23 mm and 15.17 mm, respectively. P. hypophthalmus injected with L. acidophilus cells and cell free extract following challenged with A. hydrophila cells showed survival rate of 70% and 60% respectively, at 2-week post challenged. The gas chromatography-mass spectrophotometry (GC-MS) result revealed that a diverse of compounds was detected in both the L. acidophilus cells and cell free extract, among them the most abundant component was pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl), which showed a promising anticancerous activity and might be played a significant role in the recovery of the infectious P. hypophthalmus. The current study revealed that both cells and cell free extract of L. acidophilus have antimicrobial activity against A. hydrophila.
UJI TOKSISITAS AKUT EKSTRAK ETANOL DAUN MALAKA (Phyllantus emblica) TERHADAP MENCIT (Mus musculus). (Acute Toxicity Test of Ethanolic Extract of Malaka (Phyllantus emblica) Leaves on Mice (Mus musculus)) T. Armansyah TR; Sudi Indriany; Amalia Sutriana; Rosmaidar Rosmaidar; Nuzul Asmilia; Budianto Panjaitan; Dwinna Aliza; Hamdan Hamdan
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (85.831 KB) | DOI: 10.21157/j.ked.hewan.v10i2.5137

Abstract

ABSTRACT The aim of this research was to asses the acute toxicity of ethanolic extract of malaka leaves using lethal dose 50 (LD50) on mice (Mus musculus). Twenty male mice weighing between 20-30 g were randomly divided into 4 groups (group K1-K4) of 5 mice each. All mice in group K1, K2, K3, and K4 were administered ethanolic extract of malaka leaves with the dose of 2, 4, 8, and 16 g/kg bw, respectively. Single dose of ethanolic extract of malaka leaves were given by oral gavage prior to clinical observation . The observation period was 14 days post administration, for sign of toxicity symptom, weight loss, and mortality. The result showed that no mortality was observed in the experimental animals during this study. Slight reduction of body weight was observed in group K2, K3, and K4, and no toxicity sign was found during fourteen days of observation. The LD50 of ethanolic extract of malaka leaves was higher than 16 g/kg body weight, thus, the substance was practically non toxic substance.
Characterization of Inhibin from Culture and Non Culture of Granulose Cells for Monoclonal Antibody of Inhibin Production Amiruddin Amiruddin; Tongku Nizwan Siregar; Amalia Sutriana; Dwinna Aliza; T. Armansyah
Jurnal Kedokteran Hewan Vol 4, No 1 (2010): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (486.224 KB) | DOI: 10.21157/j.ked.hewan.v4i1.9789

Abstract

This study has long-term objectives to obtain immunogenic prototype that can be used to induce multiple ovulation in goats. Working steps of this study were begun with the collection of ovarium from goats, collection of granulose cells, culture of granulose and characterization of molecular weight and isoelectric point (pI) of inhibin protein of granulose cells obtained from culture and non-culture of granulose cells, and followed by preparation of monoclonal antibody toward inhibin. The results showed that inhibin isolated either from culture or non-culture of granulose cells produced a 32 kDa band. Molecular weight of inhibin was measured by Western Blot. The 32 kDa band of SDS PAGE product appeared on Western Blot result was inhibin molecules produced by granulose cells collected fom culture and non-culture of granulose cells that can be identified by Mab-inhibin. Product of IEF gel electrophoresis suggested that inhibin molecule collected from culture of granulose cells has no charge at isoelectric points ranging from 5-6, depends on its total amino acid composition.
The Effect of Ethanol Extract of Malacca Leaves (Phyllanthus emblica) on The Number of Fibroblast Cells in White Rats (Rattus norvegicus) Burns Wound Ajirni Ajirni; Nuzul Asmilia; Nazaruddin Nazaruddin; Amalia Sutriana; Dian Masyitha; Muhammad Isa
The International Journal of Tropical Veterinary and Biomedical Research Vol 5, No 2 (2020): Vol. 5 (2) November 2020
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.63 KB) | DOI: 10.21157/ijtvbr.v5i2.20484

Abstract

Burns are caused by heat exposure, such as fire, radiation, electricity or chemicals that can damage the skin and affect the body's systems. The aim of this study was to find out the effect of the ethanol extract of Malacca leaves (Phyllanthus emblica) on the number of fibroblast cells in white rats (Rattus norvegicus) that have burned. This study used 24 white rats (Rattus norvegicus) were divided into 4 groups that smeared with aquadest as a negative control (P1), 5% ethanol extract gel of Malacca leaves (P2), 10% ethanol extract gel of Malacca leaves (P3), and positive control applied with bioplasenton® gel (P4). The IIA degree burn were created by placing a 2x2 cm hot iron plate on the back of the rat for 5 seconds. The euthanasia performed to all animal and the skin samples were collected after 15 days of treatment. Then histopathological preparations were made using HE staining. The number of fibroblast cells were analyzed by ANOVA test. The average number of white rats (Rattus norvegicus) fibroblast cells that suffered burns P1 (negative control) had a number of 7 ± 1.4 cells/visual. Whereas th e P2 group had a number of 4.2 ± 1.58 cells/visual. This value has a significant difference with the negative control. But the P2 and P3 values (3 ± 1.51 cells/visual) there is no significant different with the P4 value (positive control) with an average number of P4 fibroblast cells were 2 ± 0.4 cells/visual. The results of this study concluded that the ethanol extract of malacca leaves 5% and 10% had an effect againts accelerating burns healing in white rats ( Rattus norvegicus).
THE EFFECT OF WHOLE SEED (Barringtonia racemosa) WATER EXTRACT ON ERYTHROCYTE, HEMOGLOBIN AND HEMATOCRITE COUNT OF WHITE RAT (Rattus norvegicus) EXPOSED TO CIGARETTE SMOKE D Dasrul; Yayang Nuri Al Aliya; Amalia Sutriana; Nuzul Asmilia; Razali Daud
Jurnal Kedokteran Hewan Vol 17, No 2 (2023): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i2.25424

Abstract

This study aims to determine the effect of Barringtonia racemosa seed extract on the number of erythrocytes, hemoglobin levels, and the percentage of hematocrit in white rats (Rattus norvegicus) exposed to cigarette smoke. A total of 25 white rats Wistar strain, aged 3-4 months, male, weighing 180-200 g, were randomly divided into 5 treatment groups, each group consisting of 5 rats. Positive control group (KP) without exposure to cigarette smoke and without administration of Barringtonia racemosa seed extract, negative control group (KN) exposed to cigarette smoke without administration of Barringtonia racemosa seed extract. Treatment groups P1, P2, P3 were exposed to cigarette smoke and given Barringtonia racemosa seeds with doses of 50, 100, and 150 mg/kg BW/day, respectively. The extract was administered orally for 30 days. Blood collection in all groups was carried out via the orbital vein using a hematocrit pipette after the rats had been sedated with 0.2 mL of ketamine. Erythrocyte, hemoglobin, and hematocrit values were calculated using a hematology analyzer. Data were analyzed using one-way pattern analysis of variance (ANOVA). The results showed that giving Barringtonia racemosa seeds at doses of 50, 100 and 150 mg/kg/day had a significant effect (P0.05) in increasing the values of erythrocytes, hemoglobin, and hematocrit of white rats exposed to cigarette smoke compared to the control group which was not given the extract. In conclusion, the administration of Barringtonia racemosa seed extract can increase the number of erythrocytes, hemoglobin levels, and the percentage of hematocrit exposed to cigarette smoke.
1. The Sensitivity Level Of Gentamicine, Cholramphenicol and Penicillin Inhibiting The Growth Of Pseudomonas Aeruginosa Bacteria Isolate From Aceh Bull Prepunce Mada Admi; Annisa Anwar Sitorus; Rinidar Rinidar; Amalia Sutriana; Rosmaidar Rosmaidar; Sugito Sugito
Jurnal Medika Veterinaria Vol 15, No 1 (2021): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v15i1.20856

Abstract

This study aims to determine the sensitivity level of gentamicin, chloramphenicol and penicillin antibiotics to inhibit the bacterial growth of P. aeruginosa taken from preputium isolate of Aceh cattle. This Stock of Pseudomonas aeruginosa isolate from Microbiology Laboratory of FKH USK, re-identified its purity through: indole test, methyl red test, sugar test and as well as on Nutrient Broth (NB) media; incubated at 37ºC for 24 hours; observed the turbidity level of 0.5 Mc Farland solution. Then, the antibiotic sensitivity test was carried out using the method of Mueller Hinton Agar (MHA). The observations were made by measure the inhibition zone using the calipers in millimeters (mm) against antibiotics. The results of the observation of the inhibition zone on the gentamicin antibiotic was averaged of 25.5 mm, 23.7 mm chloramphenicol, and 12.1 mm penicillin. This study concluded that the gentamicin, chloramphenicol, and penicillin antibiotic were still effective against P. aeruginosa bacteria with the highest level of sensitivity seen in gentamicin antibiotic. 
Co-Authors Abbas, Muhammadar Abdullah ABDELBAGI, MOHAMMED AHMED ELSHAREF Abdul Harris Abdullah A. Muhammadar Abidin, Muchlisin Zainal Aftari, Nailul Ahmad Baihaqi Ahsan, Muhammad Maulana Aini, Hanipah Aisyah Fadillah Tunnisa Ajirni Ajirni Akter, Mst Nahid Aliya, Yayang Nuri Al Amiruddin Amiruddin Amiruddin Amiruddin Annisa Anwar Sitorus Anwar Anwar Arman Sayuti Arman Sayuti Arman Sayuti Arman Sayuti Asmawati Asmawati Azzahra, Putri Batubara, Agung Setia budianto panjaitan Budianto Panjaitan Budianto Panjaitan Budianto Panjaitan Budianto Panjaitan Budianto Panjaitan Cut Nila Thasmi Cut Nila Thasmi, Cut Nila D Dasrul Darmawi Darmawi Dasrul Dasrul Dasrul, D Dedi Fazriansyah Putra Dian Masyitha Dwina Aliza Dwinna Aliza Dwinna Aliza Dwinna Aliza Elza Savitri Erdiansyah Rahmi Erdiansyah Rahmi Erdiansyah Rahmi Erina Erina Erina Erina Fakhrurrazi Fakhrurrazi Fakhrurrazi Fakhrurrazi Farid Wajidi Farida Farida Feby Fema Amzani Nasution Firdus Firdus Firdus Firdus Fuza Khoiriah Gani, Azhari Gholib Gholib, Gholib Ginta Riady Ginta Riady Hafizuddin Hafizuddin Hafizuddin Hafizuddin HAFIZUDDIN, UMAR PUTEH Hamdan Hamdan Hamdan Hamdan HANUM, FARAH FARIDA Hasan, Denny I. Henni Vanda HENNIVANDA HENNIVANDA Herrialfian . Herrialfian, Herrialfian Husnurrizal . Husnurrizal, Husnurrizal Indriany, Sudi Ismail Ismail Iwan Hasri Juli Melia Juli Melia KARTINI ERIANI Karunita Dewi Khairan Khairan Mada Admi Mahdi Abrar Masda Admi Mentari Azhari L Mira Ayu Lestari Hasibuan Mohd Nor, Siti Azizah Mst Nahid Akter Muchlisin Zainal Abidin Mudatsir Mudatsir Muhammad Ammar Muhammad Hambal Muhammad Hambal Muhammad Hanafiah Muhammad Hanif Muhammad Isa Muhammad Reza Mulyadi Adam Mulyadi Adam, Mulyadi Nada Sarah Syahputri Nazaruddin Nazaruddin Nazaruddin Nazaruddin Novianti, Arindita Niatazya Nurhazimah Nurhazimah Nurliana NURLIANA NURLIANA Nuzul Asmilia Nuzul Asmilia Nuzul Asmilia Putra, Dedi Fazriansyah Qathrinnada Ramadhana R Roslizawaty R Roslizawaty, R Rahmad Rahmad Rasmaidar . Raysa Syarifah Razali Daud Razali Daud rina aulia barus Rinidar Rinidar Roshada Hashim Roshada Hashim Roslizawaty Roslizawaty Roslizawaty Roslizawaty Roslizawaty Roslizawaty, Roslizawaty Rosmaidar Rosmaidar Rosmaidar Rosmaidar, Rosmaidar Rossy Septia Putri S Syafruddin Sail, Asmawati Muhammad Sari, Wahyu Eka Sembiring, Rinawati Siddik, Tasya Luthfiah Siregar, Tongku N. Siti Aisyah Siti Azizah Mohd Nor Sri Wahyuni Sri Wahyuni Sriyanti, Cut Sudi Indriany Sugito Sugito Syafruddin Syafruddin Syafruddin Syafruddin Syafruddin Syafruddin Syafruddin, S T Armansyah TR T. Armansyah T. Armansyah T. Armansyah Teuku Armansyah Teuku Armansyah Titit, Marcelus Ferdinandez Tongku N. Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar, MP Triva Murtina Lubis Winaruddin Winaruddin Yayang Nuri Al Aliya Yusmadi Yusmadi Zakwan, Muhammad