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All Journal Jurnal Civics: Media Kajian Kewarganegaraan Jurnal Pendidikan Kewarganegaraan Undiksha KELOLA Al-Adzka: Jurnal Ilmiah Pendidikan Guru Madrasah Ibtidaiyah JURNAL JPSD (Jurnal Pendidikan Sekolah Dasar) Jurnal Studi Agama dan Masyarakat Jurnal Ilmiah Pendidikan Pancasila dan Kewarganegaraan Indonesia Performance Journal Jurnal Obsesi: Jurnal Pendidikan Anak Usia Dini Jurnal Moral Kemasyarakatan JMM (Jurnal Masyarakat Mandiri) JCES (Journal of Character Education Society) Etika Demokrasi Jurnal Pelita PAUD Journal of Moral and Civic Education Cakrawala: Jurnal Litbang Kebijakan Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Journal of Civic Education Jurnal Fundadikdas (Fundamental Pendidikan Dasar) Buletin KKN Pendidikan Jurnal Ilmu Manajemen Profitability Al-Muamalat: Jurnal Hukum dan Ekonomi Syari'ah MURHUM : JURNAL PENDIDIKAN ANAK USIA DINI Jurnal Cahaya Mandalika Jurnal Pengabdian Masyarakat Indonesia Jurnal Pendidikan PKn Wasana Nyata Jurnal Kewarganegaraan Jurnal Ar-Ribh Winter Journal: IMWI Student Research Journal Atthufulah : Jurnal Pendidikan Anak Usia Dini Journal Online Manajemen ELPEI (JOMEL) Journal of Halal Science and Research Jurnal Kebidanan dan Keperawatan Aisyiyah Jurnal Pemberdayaan Sosial dan Teknologi Masyarakat Eduvest - Journal of Universal Studies Jurnal Inovasi Pendidikan WASANA NYATA SmartComp Jurnal Edukatif Jurnal Pengabdian Sosial Jurnal Healthy Mandala Waluya Asian Journal of Social and Humanities Jurnal Kefarmasian Indonesia Greenation International Journal of Economics and Accounting Jurnal Pelita PAUD Jurnal Pendidikan Tuntas Jurnal Info Kesehatan Arunika: Jurnal Pengabdian Masyarakat
Hariyanti Hariyanti
Nursing Department, Polytechnic Health Ministry Of Semarang
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Journal : Journal of Halal Science and Research

 1 
The study of pig dna analysis methods in cosmetics: a review Sofiyani, Ratu Amada; Hariyanti, Hariyanti; Rahmi, Hanifah
Journal of Halal Science and Research Vol. 2 No. 1 (2021): February
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (761.76 KB) | DOI: 10.12928/jhsr.v2i1.5177

Abstract

Collagen is one of the components used for cosmetics. Functionally, collagen is derived from pig fat, which functions as a skin conditioning agent (Emollient), emulsion stabilizer (Emulsifier), and as ingredient to increase the viscosity of cosmetic preparations. The method that can detect pig content is the PCR method, which can confirm the presence of DNA content in pigs. In this study, an analysis of cosmetic products containing porcine DNA was carried out using the PCR method. This research was divided into two stages: the initial stage was DNA extraction, and the second stage was PCR analysis. This research is a study through literature study using the narrative review technique. This study aimed to collect information and examine the analysis method of identifying pig DNA contained in cosmetics to obtain the right and accurate way through a literature approach. From the literature study results, it can be concluded that the best extraction method for the isolation of porcine DNA in cosmetic preparations is the extraction method with the Boom Method, while the DNA analysis used to identify porcine DNA in cosmetics is RT-PCR (Real-time Polymerase Chain Reaction). Conventional PCR is because both methods have their respective advantages. The RT-PCR (Real-time Polymerase Chain Reaction) method can amplify and quantify the number of target DNA molecules. The Conventional PCR Method can form DNA bands based on the amplification value. 
Narrative review: a study of pork DNA analysis methods in food gelatin Ansor, Nisa Aulia; Hariyanti, Hariyanti; Rahmi, Hanifah
Journal of Halal Science and Research Vol. 4 No. 2 (2023): September
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jhsr.v4i2.7798

Abstract

Gelatin is a substance resulting from the partial hydrolysis of collagen consisting of animal cartilage, skin, and bones from water-soluble polypeptides. The problem formulation in this study is what DNA analysis methods can be used to detect pig DNA in food gelatin. This study aimed to collect, review, and conclude information regarding the most widely used DNA analysis method to detect pig DNA in food gelatin. From the results of the literature study, it can be concluded that the most widely used extraction method in the analysis of porcine DNA in food gelatin is the DNeasy® Mericon Food kit extraction method for good purity results, there is the foodproof® III Kit method, the universal Wizard KIT Promega®, the DNeasy® Mericon Food kit, and the Column based FavorPrep™ Food DNA Extraction Kit. The pig DNA analysis method is widely used in conventional PCR. Sensitivity, fast testing time, and gh acporcine gelatin sample was identified by the appearance of a band on the electrophoresis results. Keywords: Food Gelatin, Extraction Method, DNA Analysis, Journal Review.
Determination of gelatin from marshmallows using a combination of fourier transform infrared (ATR-FTIR) spectroscopic and chemometrics for halal authentication Fatmawati, Sofia; Hariyanti, Hariyanti; Permanasari, Etin Diah; Chandra, Ady; Firmanto, Johan Dwi; Sari, Retno Lia; Pamungkas, Septiana Tri
Journal of Halal Science and Research Vol. 5 No. 2 (2024): September
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jhsr.v5i2.10537

Abstract

Marshmallow is a foam that contains aerated sugar, which is stabilized with gelatin or egg albumin. In this research, a Fourier Transform Infra-Red (ATR-FTIR) spectroscopy combined with the Chemometrics methods is developed to distinguish the presence of porcine gelatin in marshmallows, which can then be used to identify halal products. This method provides fast and rapid testing of halal products. Marshmallows were made with varying concentrations of bovine and porcine gelatine as the reference. Commercial marshmallows were collected in the online marketplace by purposive sampling. Isolated gelatin was analyzed using the ATR-FTIR spectrophotometer, and data analysis was continued using PCA (Principal Component Analysis). The results showed that bovine gelatin absorbed at a wavenumber of 1638 cm-1, while porcine gelatin produced a sharp absorption at 1697 and 1654 cm-1. The results of PCA analysis show that 100% of bovine gelatin marshmallows (S100) have areas different from marshmallows containing a mixture of porcine gelatin. The PCA results of four samples (A1, A2, A3, and A4) show they are in the same area as 100% bovine gelatin marshmallows (S100). This shows that it is suspected that the four marshmallow samples tested did not contain porcine gelatin. The multivariate regression curve showed that the pattern of linear absorbance changes along with porcine gelatin concentrations with the highest coefficient equation is from wavenumber 1093.97 cm-1.   Keywords: ATR-FTIR, Bovine gelatin, Chemometric, Marshmallow, Porcine gelatin
Co-Authors Abdullah Hasibuan, Hamdi Afriyani Afriyani Agus Utomo Ahmad Eddison Ahmad Muhibbin Aisyah Aisyah Al Rafni Alam, Andi Rifqah Purnama Amalia Amalia Angraini, Risa Dwi Anisa, Nurul Ansor, Nisa Aulia Apriani, Triana Ardestya Linanjung, Yolanda Arianti, Sri Dita Aris Munandar Artiyany, Megawhati Asmawiyah Asmawiyah Ayu Safira, Ayu Azzahra, Khadijah Brotojoyo, Endang Budi Halomoan Siregar Cecep Darmawan, Cecep Chandra, Ady Charisma, Shintia Lintang Cindy Cinthia Dadang Sundawa, Dadang Dary DN, Widia Ulan Delvia, Elyca Dian Nurmastuti Dini Handayani, Dini Djulaeka, Djulaeka Endang Saryanti Etin Diah Permanasari Fakhrana, Ayu Fatmawati, Sofia Fatmawati, Yuliana Indah Febriansyah Febriansyah Filma Alia Sari Firmanto, Johan Dwi Gantini, Sri Nevi Grosman, Kingkel Panah Gumilang, Intan Hadi Sunaryo Hambali Hambali Hamidiyanti, Baiq Yuni Fitri HANIFAH RAHMI Harahap, Ramona Hasanah, Hikmatun Hikmah, Ikmalul Husnul Khatimah I Made Agus Oka Gunawan Ika Yuni Astuti Indah Handayani, Wiwik Irayanti, Irma Irayanto, Irma Ismayani, Widya Istianah, Anif Judijanto, Loso Jumili Arianto Kalista, Anggia Kamalia, La Ode Kapriani Kapriani, Kapriani Kristianto, Jusuf L, Andreas Utomo Lasty Agustuty Leni Anggraeni Mahmudi, Bambang Manurung, Intan Zarina mariyani, dede Masganti Sit Masyitoh, Iim Siti Mufaqqih Amrullah, Bashirotul muhammad khoiruman, muhammad Muhammad Yusuf Murni Sulistyowati, Murni Muryati Muryati Nafi’ah, Khusnun Nashiroh, Annisa Laylatun nasution, candri wulan Nasution, Ummi Hani Novida, Diah Rachmawatie Nunuk Herawati Nur Azizah Nur Fatwa Basar NURJAYA NURJAYA, NURJAYA Nurlinda Nurlinda, Nurlinda Nuryanah, Nuryanah Nuryati Nuryati Pamungkas, Septiana Tri Permady, Gigieh Cahya Pramudika, Risang Galih Primahadi, Indra PRIYANTI, DHEA Putra, Christophorus Indra Wahyu Putri, Hilya Putri, Riska Amelia Radini, Radini Rahmat Rahmat Ratna Umi Nurlila Rina Ani Sapariyah Rizki Aulia Saefuloh, Irfan Saepudin Karta Sasmita Saputra, Ridwan Cahya Saputri, Aulia Indah Saragih, Sri Rahmah Dewi Sari Nurlaily, Sari Sari, Novriani Rahma Sari, Putri Lidiana Permata Sari, Retno Lia Sari, Wiwin Novita Sasmita, Saepudin Karta Sawitri, Nonik Wulan Septiana Novita Dewi Setiyawan, Sukamto Jodi Sholihah, Husna Imro’atush Siahaan, Matdio Sinambela, Helen F Siregar, Widia Friliska Sofiyani, Ratu Amada Subagio, Mochammad Suciati, Raihana Tresna Suhendra, Agus Suryanef Suryanef Suryani Manurung Susanti, Nani Irma Syabrina, Muhammad Thaha, Salmiyah Utama, Heriyanta Budi Wariati, Ambar wati, Rizki amalia Wibowo Heru Prasetiyo Widaningsih Widaningsih, Widaningsih Widiarochmawati, Niniek Yasin, Nurfatwa Andriani Yesica Siahaan, Chrystin Yudi Supiyanto Yunarta, Farizki