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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences REKA RACANA TOMALEBBI: Jurnal Pemikiran, Penelitian Hukum, Pendidikan Pancasila dan Kewarganegaraan (PPKn) Jurnal Mirai Management Jurnal Ilmiah Universitas Batanghari Jambi INTECOMS: Journal of Information Technology and Computer Science SEIKO : Journal of Management & Business Jurnal Redoks Jurnal Media Analis Kesehatan Jurnal Keperawatan Komprehensif (Comprehensive Nursing Journal) Jurnal Agronomi Tanaman Tropika (JUATIKA) Contagion: Scientific Periodical Journal of Public Health and Coastal Health Abdimas Universal Jaringan Laboratorium Medis Jurnal Ilmu Pemerintahan Suara Khatulistiwa Civil Service : Jurnal Kebijakan dan Manajemen PNS Al-Ahkam : Jurnal Hukum Pidana Islam Akrab Juara : Jurnal Ilmu-ilmu Sosial JURNAL ILMU PERPUSTAKAN (JIPER) Enggang: Jurnal Pendidikan, Bahasa, Sastra, Seni, dan Budaya Jurnal Cakrawala Mandarin Joong-Ki : Jurnal Pengabdian Masyarakat Transformasi: Journal of Economics and Business Management DIKTUM: JURNAL SYARIAH DAN HUKUM Jurnal Manajemen Riset Inovasi Seminar Nasional Hasil Penelitian LP2M UNM Jurnal Manajemen Dan Bisnis Ekonomi Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Citra Delima Scientific journal of Citra Internasional Institute Pusat Publikasi Ilmu Manajemen Jurnal Ilmiah Research Student Jurnal Informatika Polinema (JIP) JURNAL ILMIAH PENELITIAN MAHASISWA Joong-Ki Jurnal Pendidikan Teknologi Informasi (JUKANTI) Dinamika Pertanian Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal in Teaching and Education Area Jurnal Teknik Sipil Hadlonah : Jurnal Pendidikan dan Pengasuhan Anak Jurnal Cendekia Ilmiah Jurnal Ekonomi, Manajemen, Akuntansi Joong-Ki Perspektif Administrasi Publik dan hukum Dialogika : Jurnal Penelitian Komunikasi dan Sosialisasi Proceeding International Collaborative Conference on Multidisciplinary Science
Herman Herman
Fakultas Ilmu Sosial Universitas Negeri Makasar
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The DNA Sequence Encoding Actin (ACT1) of Pandan (Benstonea sp.) Dewi Indriyani Roslim; Herman Herman
Journal of Tropical Life Science Vol. 9 No. 3 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.03.06

Abstract

One of housekeeping genes is actin gene. This gene is frequently used in gene expression studies as an internal control. The DNA sequence encoding actin from Pandan (Benstonea sp.) clone Riau has not been reported, therefore, this study investigated the DNA sequence encoding actin isolated from Benstonea sp. clone Riau. Total DNA isolation was performed in fresh leaves, total RNA isolation from stem, total cDNA synthesis, polymerase chain reaction using degenerate actin primer, electrophoresis, cloning, transformation, blue white colony selection, colony PCR, sequencing, data analysis using BioEdit and MEGA6 softwares and BLASTn program. The partial DNA sequence encoding actin from Benstonea sp. clone Riau obtained was 1,403 bp. The sequence was grouped as part of actin1 (ACT1) and it was consisted of two exons and one intron. The predicted coding and peptide sequences were 616 bp and 205 amino acids, respectively.  The predicted coding sequence had 90% similarity to some ACT1 mRNA from some plants species but none of which belongs to Benstonea genus or Pandanaceae family. The deduced peptide sequence had similarity to some ACT1 peptide from some plant species of up to 99% and also none of them belongs to Benstonea genus or Pandanaceae family. Thus, the partial ACT1 gene obtained in this study was the first sequence reported from Benstonea genus. Furthermore, this sequence can be used as a reference to isolate actin genes from other species within Benstonea genus for gene expression analysis purposes.
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