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Herman Herman
Fakultas Ilmu Sosial Universitas Negeri Makasar
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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The DNA Sequence Encoding Actin (ACT1) of Pandan (Benstonea sp.) Dewi Indriyani Roslim; Herman Herman
Journal of Tropical Life Science Vol. 9 No. 3 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.03.06

Abstract

One of housekeeping genes is actin gene. This gene is frequently used in gene expression studies as an internal control. The DNA sequence encoding actin from Pandan (Benstonea sp.) clone Riau has not been reported, therefore, this study investigated the DNA sequence encoding actin isolated from Benstonea sp. clone Riau. Total DNA isolation was performed in fresh leaves, total RNA isolation from stem, total cDNA synthesis, polymerase chain reaction using degenerate actin primer, electrophoresis, cloning, transformation, blue white colony selection, colony PCR, sequencing, data analysis using BioEdit and MEGA6 softwares and BLASTn program. The partial DNA sequence encoding actin from Benstonea sp. clone Riau obtained was 1,403 bp. The sequence was grouped as part of actin1 (ACT1) and it was consisted of two exons and one intron. The predicted coding and peptide sequences were 616 bp and 205 amino acids, respectively.  The predicted coding sequence had 90% similarity to some ACT1 mRNA from some plants species but none of which belongs to Benstonea genus or Pandanaceae family. The deduced peptide sequence had similarity to some ACT1 peptide from some plant species of up to 99% and also none of them belongs to Benstonea genus or Pandanaceae family. Thus, the partial ACT1 gene obtained in this study was the first sequence reported from Benstonea genus. Furthermore, this sequence can be used as a reference to isolate actin genes from other species within Benstonea genus for gene expression analysis purposes.
Co-Authors Adelia Ramadani Adriana Airlia Yusrin Ahmar, Ansari Saleh Amirul Mukminin Andi Asripan Andi Ceri Susilawati ANDI FIRMANSYAH Andi Fitria Ramadhani Arifuddin Usman Arismunandar Arismunandar Armansyah Armansyah Arusmalem Ginting Asri Ainun Bakhtiar Asri Wahyuni Asti Besse Arwan Betty Leindarita Chris Tan Daniel Frengky Kamengko Dewi Indriyani Roslim Dewi Indriyani Roslim Dian Thifany Ramadhania Diana Vimalakirti Chow Duma Simanjuntak Dwi Novelia Dwi Novelia Edianto Karo Karo Eko Murti Saputra Elfrida Oktavia Endah Budi Lestari Erti Manurung Fadel Nugraha Fathurrahman Fathurrahman Fauzan Alfikrie Fauzan, Suhaimi Fiona Fiona Fitmawati Fitmawati Frans Panjaitan Fuad Nur Fulgentius Danardana Murwani Giavani Giavani Hamka Hamka Hana Monica Hana Monica Hardi Suandri Hercules Gultom Herdaningsih, Sulastri Ibunga Tang Ida Rindaningsih Imam Mukhlis Imam Suyitno Indah Mulyasari Indah Sari Irmawati Alimuddin Isry Laila Syathroh Kalma Kalma Kalvin Lee Kelvin Yovanka Khuzanah Khuzanah Kusbaryanto La Ode Muhamad Sulihin La Ode Muhamad Taufiq Afoeli Lade Sirjon Lade Sirjon Lara Julia Trisantika Lara Julia Trisantika M Syafnur M. Dimas Bagus Nurmuzayyin Marina Lidya Marsha Putri Maulia Marwah Marwah Marwawati Marwawati Maryati Maryati Maya May Syarah Mita Mita Muarif Leo Mudji Suhardiman Muh. Yoghy Wana Putra Muhammad Ibnu Khatan Muhammad Irvan Nur Iva Muhammad Isa Alamsyahbana Mustafa Mustafa Netania Saragih Nguyen Van Thao Nurdin Nurdin Nurdin Nurdin Nurul Amalia Nuryanti Mustari Oka Purwanti Okyarni Nuzila Prissilia Angelika Purba, Elprinawati Purwoko, Agus Putri Ade Rahma Yulis Rahmi Rahmi Resfika Aswira Reski Rahayu Ricky Ricky Rosmiaty Djamaluddin Rukiah Rukiah Sapto Haryoko Sari Widianti Selvia Andita Sholahuddin Irsyad Siagian, Claudya Benesa Simanjuntak, Novita Siska Andriani Sitompul, Heppy Theresia Sopiah Sopiah Sri Wahyuni Astuti sunardi sunardi Syifa Riski Ardiningtias Ardiningtias Tomi Vincent Tri Frida Suryati Vitalia Hanako Murni Simanjuntak Wahyudi Wahyudi Widarti Widarti Winda Ayu Virginia Yandi Suprapto Yefta Christian Yolla Putri Ardila Yuritanto, Yuritanto