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Journal : Indonesian Journal of Biotechnology

Adherence Pheno-genotypic of Escherichia coli O157:H7 Isolated from Beef, Feces of Cattle, Chicken and Human I Wayan Suardana; Wayan Tunas Artama; Widya Asmara; Budi Setiadi Dayono
Indonesian Journal of Biotechnology Vol 16, No 1 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1601.412 KB) | DOI: 10.22146/ijbiotech.15990

Abstract

Generally, adherence of micro-organisms to host cells is the frst step of the colonization to host surfaces.Escherichia coli O157:H7 can colonize to the intestine and induce attaching-effacing (AE) lessions. The capacityof inducing AE lesions is encoded by a pahtogenicity island, the locus of enterocyte effacement (LEE) thatcontains genes involved in generation of attaching and effaching (A/E) lesions. Among which that, the eaegene is encoding intimin, an outer membrane protein that is responsible to intimate attachment to the intestinalepithelial cells. A total of 20 local isolates obtained from human clinically, beef, cattle, chicken, and humannon-clinically were tested to adherence pheno-genotypic of E. coli O157:H7. The eae gene was identifed usingpolymerase chain reaction with a specifc primer i.e AE19 forward and AE20 reverse. To confrm phenotypicof gene, further study was performed by culturing the bacteria in vero cell, followed by Giemsa staining andAcridine Orange Fluorescent staining 3 h and 6 h after incubation, respectivelly. Result of study showed thatthere were 19 out of 20 (95%) isolates identifed positive eae gene. Giemsa staining appeared that the bacteriawith positive eae gene performed a cluster arroud of cell (localized adherence). On the other hand, the negativeeae gene appeared as a diffuse adherence (DA). The study indicated that almost all of E. coli O157:H7 localisolates which was positive eae gene had potency to colonize to the intestine and induce attching-effachinglessions, and also cause cytopahatic effects in intestinal epithelial cell
Co-Authors Abdul Manan Achjar, Komang Ayu Henny Adirinata, I Komang Pasek Agus Sri Lestari Amrulloh, Muhammad Faqih Anak Agung Gde Bagus Udayana Anderson Ngelambong Andi Isma Lestari Amin, Andi Isma Anom Hery Suasapha Ardika, I Gusti Ngurah Putu Ari Yuniastuti Aribaten, Ni Nengah Zinnia Arimbawa, I Gede Artayani, Ida Ayu Gede Asyauqi Ilham Perdana, Asyauqi Ilham Aungsuroch, Yupin Bambang Sumiarto Bangun Mulia, Victor Bq Nurlita Anugrah BUDI SETIADI DARYONO Budi Setiadi Dayono Chandra Yowani D. A. Widiasih Denny Widaya Lukman Devi Dwi Jayanti Dewa Gede Agung Widyadnyana Dewa Putu Oka Prasiasa Dewi, Ni Nyoman Astika Dewi, Ni Putu Diah Trisna Dewintasari, Ni Nyoman Paramitha Dwi Lestari Dyah Ayu Widiasih Dyah Ayu Widiasih Dyah Ayu Widiasih Eka Putri Suryantari Emmanuella Felice’anna Dije Karisoh Febri Diana Putri, Ni Putu Febrianti, Andri Nurdiana Franciska, Juliana Gama, I Ketut Gama, Ketut Gede Ngurah, I Gusti Ketut Gejir, I Nyoman Gusti Ayu Marhaeni Hana Kristal Alamanda Septiara Harini, I Gusti Ayu Hartati, Ni Nyoman Henny Achjar, Komang Ayu Hikam, Ahdan Sayid I Dewa Made Sukrama I G. Wijana I Gusti Agung Ayu Suartini I Gusti Made Krisna Erawan I Gusti Putu Bagus Sasrawan Mananda I Ketut Adi Sugita I Ketut Mangku Budiasa I Ketut Muka I Ketut Suada I Ketut Suardana I Ketut Suatha I Komang Gede Wiryawan I Made Adikampana, I Made I Made Kardena I Made Mertanadi I Made Sukada I Made Sukarja I Made Walesa Putra I Nengah Kerta Besung I Nengah Sujaya I Nengah Wirakesuma, I Nengah I Nyoman Ariana I Nyoman Suarsana I Nyoman Sudiarta I Nyoman Sukma Arida I Nyoman Sunarta I Nyoman, suardina I Putu Sampurna I Putu Sudiarta I Wayan Adnyana I Wayan Mustika I Wayan Seriyoga Parta I. H. U Utama Ida Bagus Ngurah Swacita Iga Prassetyo Adji, Iga Prassetyo Ignatius Cahyanto INDAYATI LANYA Iwan Harjono Utama Karuni, Ni Kadek Khamid Yusuf Baehaqi, Khamid Yusuf Komang Januartha Putra Pinatih Kondra, I Wayan Korbinianus Feribertus Rinca Kumalasari, Ni Putu Putri Kusumajaya, Anak Agung Ngurah Laba, I Nyoman MAS DJOKO RUDYANTO Meitisrilatifatulain Fitriadewi Mariana Michael Haryadi Wibowo Mita Ekamelinda Mochamad Choirul Hadi Ngurah, I Gusti Ketut Gede Ngurah, IGK Gede Ni Kadek Ari Divania Widia Artha Ni Kadek Lyming Lestari Ni Ketut Suwiti Ni Luh Sustiawati Ni Luh Watiniasih Ni Made Ayu Aryati Dinarini Ni Made Inna Dariwardani Ni Made Ruastiti Ni Putu Ratna Sari Nur Habibah Nuria Fitrianti Putri Nyoman Dewi Pebryani NYOMAN SEMADI ANTARA Oktivia Chandra Mustika, Oktivia Chandra P. Sampurna Padmi, Luh Sri Anggayoni Julia Pitriyantini, Putu Eka Prabhadewi, Ni Putu Sriarta Pramesti, Kadek Diah Pratiwi, Ida Ayu Windhari Kusuma Putra, I Kadek Aldi Margareta Perdana Putu Agus Wikanatha Sagita Putu Ayu Sisyawati Putriningsih Putu Cahaya Semesta Putu Januari Ratna Apsari Putri Putu Sucita Yanthy R Susanti Raharjo, Anis Rahmasari, Ni Nyoman Putri Asri Rasdianah Rasdianah Remawa, Anak Agung Gede Rai Reny Navtalia Sinlae Rian Ka Praja Ribek, Nyoman Richard Christian Daud Ruspawan, Dewa Made Ruta, I Made Satriawati, Ni Nyoman Ayu Sipahutar, Ida Erni Siti Helmyati Sri Anggreni Lindawati Sri Wahyuni Suarya Putra, I Nyoman Agus Sudiantara, I Ketut Sudiantara, Ketut Suharsono, Hamong Sukoco, Hendro Sulisnadewi, Ni Luh Kompyang Sunatha Putra, Agus Surasak Jamnongsarn, Surasak Surya, I Kadek Adi Syamsul Alam Paturusi Tjokorda Sari Nindhia Totton, Mary Louise Victor Bangun Mulia Wahyu Hananto, Wahyu Wayan Tunas Artama Wayan Tunas Artama Wedri, Ni Made Widya Asmara Wilantari, Ni Nyoman Ayu Wulandari, Andi Dewi Wulandari, Kadek Dina Yan Ramona Yohanes Kristianto Yuli Darmawan, Yuli Yunita Sri Hastuti, Yunita Sri