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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA MEDIA KONSERVASI Hemera Zoa Jurnal Sain Veteriner Jurnal Veteriner JURNAL ANATOMI FISIOLOGI Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Peternakan Indonesia JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Bioma : Jurnal Biologi Indonesia Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) ARSHI Veterinary Letters FITOFARMAKA: Jurnal Ilmiah Farmasi EKOLOGIA : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup JFIOnline Jurnal Ilmu Kefarmasian Indonesia JCPS (Journal of Current Pharmaceutical Sciences) Jurnal Aisyah : Jurnal Ilmu Kesehatan Journal of Parasite Science Jurnal Kedokteran Hewan Jurnal Veteriner Nusantara
HERA MAHESHWARI
IPB University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Engineering Environmental Science Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Nursing Public Health Veterinary Other

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SEKUENSING GEN MCR-1 DARI ESCHERICHIA COLI DAN SALMONELLA ENTERITIDIS RESISTAN KOLISTIN Maria Fatima Palupi; Ernes Andhesfa; Hera Maheshwari; Huda Salahuddin Darusman; Etih Sudarnika; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Gen mobilized colistin resistance (mcr) merupakan gen resistan kolistin sulfat yang bisa dipindahkan melalui materi genetik bergerak misalnya plasmid. Tujuan dari penelitian ini adalah mengetahui jenis plasmid gen mcr-1 yang berhasil dipindahkan dari satu isolat Escherichia coli resistan kolistin ke Salmonella Enteritidis ATCC 13076 (dua isolat), dan satu isolat E. coli O157:H7 resistan kolistin positif gen mcr-1. Sekuensing gen mcr-1 kemudian dianalisis di unit Biotek Balai Besar Pengujian Mutu dan Sertifikasi Obat Hewan dengan menggunakan basic local alignment search tools (BLAST) dan software MEGA7. Hasil sekuensing gen mcr-1 dengan panjang nukleotida 309 memiliki homologi tinggi dengan data referen dari Genbank pada gen mcr-1 yang terdapat dalam plasmid. Hasil sekuensing DNA gen mcr-1 sampel lebih cenderung termasuk pada tipe plasmid IncI2 atau IncHI2. Hal ini menunjukkan bahwa resistansi kolistin merupakan ancaman yang nyata karena gen resistan mcr-1 yang ada di Indonesia benar berada di plasmid sehingga mudah disebarkan ke bakteri lain. Kata kunci: mcr-1, plasmid, resistansi ABSTRACT The mobilized colistin resistance (mcr) gene is a colistin sulfate resistance gene that can be transferred through mobile genetic material such as plasmids. The purpose of this study was to determine the type of plasmid mcr-1 gene that was successfully transferred from an isolate of colistin-resistant Escherichia coli to Salmonella Enteritidis ATCC 13076 (two isolates), and one colistin-resistant E. coli O157: H7 that positive mcr-1 gene. The sequencing of the mcr-1 gene was then analyzed in the Biotek unit at National Veterinary Drug Assay Laboratory using basic local alignment search tools (BLAST) and MEGA7 software. The sequencing of the mcr-1 gene with nucleotide length 309 has a high homology with the reference data from Genbank on the mcr-1 gene contained in the plasmid. DNA sequencing of mcr-1 gene samples is more likely to belong to the IncI2 or IncHI2 plasmid types. This shows that colistine resistance is a real threat because the mcr-1 resistance gene in Indonesia is actually in the plasmid so it is easily spread to other bacteria. Keywords: mcr-1, plasmid, resistance
Maltosa Mempertahankan Viabilitas Spermatozoa Epididimis Kerbau Belang yang Disimpan dalam Bentuk Cair Yulnawati -; Hera Maheshwari; Muhammad Rizal; Herdis -
Jurnal Veteriner Vol 11 No 2 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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A study to determine the effect of maltose supplement in andromed based semen extender on theviability of buffalo’s epididymal spermatozoa following 12 and 24 hour storage as liquid semen. Epididymalspermatozoa were collected by combined methods of slicing, flushing and tissue pressure. The spermatozoawas then diluted with three different extenders, i.e Andromed only (A) as control, Andromed + 0.2% w/vMaltose (M1) and Andromed + 0.4% w/v Maltose (M2) as treatments. The percentages of progressivelymotil spermatozoa in the liquid semen in the following treatment of A, M1 and M2 were 48.33%; 53.33%and 55% respectively (after 12h of storage), and 45%; 46.67% dan 45% respectively (after 24h of storage).Besides, the percentage of live spermatozoa in A, M1 and M2 after 12h of storage were 70.33%; 72 % and72.33 % and after 24h of storage were 66.33%; 70% and 70.67 %. In conclusion, the addition of 0.2% and0.4% w/v maltose into Andromed extender could maintain the life of the spotted buffalo epididymalspermatozoa after storage for 4°C up to 24 hours.
Efektivitas Follicle Stimulating Hormone (FSH) terhadap Produktivitas dan Siklus Bertelur Ayam Lokal (EFFECTIVENESS OF FOLLICLE STIMULATING HORMONE (FSH)ON THE PRODUCTIVITY AND EGG LAYING CYCLE OF THE DOMESTIC FOWL) Maria Prihtamala Omega; Hera Maheshwari; Reviany Widjajakusuma
Jurnal Veteriner Vol 4 No 1 (2003)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

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Pemanfaatan Electronic Nose sebagai Sensor Kimiawi Urin Guna Melacak Birahi Sapi (ELECTRONIC NOSE AS URINARY CHEMICAL SENSOR FOR DETERMINING ESTROUS PHASE IN CATTLE) Pudji Astuti; Claude Mona Airin; Slamet Widiyanto; Luthfiralda Sjahfirdi; Hera Maheshwari
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The timing of artificial insemination relies largely on behavioral observation of estrus. The problemfaced is that not all cattle shows signs of estrus significantly, which affect the accuracy of insemination,and therefore,the success rate of Artificial Insemination is less than 50%. Recently, the determination ofestradiol levels as an indicator of estrus is done by observation of physical signs and doing ELISA test,which is expensive and provide longer time. In order to solve these problems, a tool estrus detector is madenamely Electronic Nose (EN). Determination of estrus with EN is cheaper because it does not need to usecomplexmaterials, just only use the samples. Mechanism of action of EN is using a sensor that is vaporized,while animals estrus will emit pheromones that are vaporized. Theaim of this study was to determinewhether the stage of estrus can be detected by using EN. Urine of female Ongole Crossbred which maintainedin Kuwang, district of Cangkringan, Yogyakarta, with BCS of 3 was used in this research. The sample wascollected shortly before injection of dinoprost as estrus synchronization then it repeated when cattle got estrus phase. The urinary sample of the estrus cattle was sensitive to methane, propane, butane, whereasin non-estrus cattle, besides the three of these component (methane, propane, butane), sensor was alsocaptured hydrogen sulfide. Furthermore, our electronic nose had been able to distinguish estrus phase andnon-estrus based on domain area.Thus, the Electronic Nose is very prospect used as a detector of estrus incattle. Hydrogen disulfide could possibly be used as an indicator comparison between cow estrus and nonestrus.
Kesetaraan Uji Mastitis IPB-1 dengan Metode Breed untuk Mendiagnosis Mastitis Subklinis pada Susu Kerbau Murrah dan Kambing (THE EQUALITY OF IPB-1 MASTITIS TEST WITH BREED METHOD FOR SUB-CLINICAL MASTITIS DETECTION ON MURRAH BUFFALO’S MILK AND GOAT’S MILK Mirnawati Bachrum Sudarwanto; Hera Maheshwari; Faisal Tanjung
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Sub-clinical mastitis causes decrease in milk production and milk quality. It is not only happen tomilking dairies, but also happens to dairy buffalos and goats. The objective of this study is to know thedifferences between IPB-1 mastitis test (IMT) and Breed method to diagnose sub-clinical mastitis ondairy buffaloes and goats. Fourty two samples of buffalo’s milk and 20 samples of goat’s milk were used tosomatic cell count (SCC) with direct and indirect method. Direct method was performed by counting themilk’s SCC with Breed method, and indirect method was performed by observing the reaction betweenIMT reagent and milk. The results showed that 28 from 42 samples (66.67%) of buffalo’s milk and 13 from20 samples (65%) of goat’s milk tested with Breed method came from the herds which suffered from subclinicalmastitis and 27 from 42 samples (64.28%) and 10 from 20 samples (50%) of goat’s milk testedwith IMT showed positive reaction. This research also showed that IMT has sensitivity of 96% and specivicityof 100% for buffalo’s milk and sensitivity of 71% and specivicity of 100% for goat’s milk. IMT can be usedto obtain fast result for sub-clinical mastitis diagnosis and it is faster and easier for buffalo’s and goat’smilk.
INDENTIFIKASI TINGKAT KESEHATAN DAN STRES KELELAWAR PEMAKAN BUAH (Cynopterus tithaecheilus) SEBAGAI MAMALIA TERBANG Anisa Rahma; Desrayni Hanadhita; Andhika Yudha Prawira; Danang Dwi Cahyadi; Supratikno Supratikno; Hera Maheshwari; Aryani Sismin Satyaningtijas; Srihadi Agungpriyono
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (117.937 KB) | DOI: 10.19087/jveteriner.2018.19.4.460

Abstract

Hematology value is important to evaluate individual health status. This study was purposed to observe the hematological values of male and female Cynopterus titthaecheilus. The study was conducted on 16 C. titthaecheilus (six adult males, six adult females and four juveniles) to study the haematological profiles. Blood samples were taken using tuberculin needles and analyzed by hematology analyzer for hematological values. Females C. titthaecheilus had a higher number of white blood cells than the males, but no differences in the white blood cells differential. Males C. titthaecheilus had a higher number of platelets than the females. Generally, the juveniles had a higher number of leucocytes as well as number of platelets than the adults. Based on the stress index value, it was concluded that young bats had a lower stress level compared to adult bats.
Correlation Analysis of Enverontmental Cd Exposure in Liver and Meat of Broiler Chicken Amelia Ramadhani Anshar; Huda Salahudin Darusman; Hera Maheshwari
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 2 NO. 2, JULY 2018
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v2i2.4354

Abstract

Cadmium (Cd) belongs to heavy metal with high anthropogenic pollutant and non-essential to physiological funtion. Regular intake of consuming Cd leads to physiological disorders in poultry animal as well as humans as one who consume it. The aim of this study was to analyze the profile of Cd content in environmental samples (poultry feed and water) and it correlated with the degree of contamination in broiler tissues. In this study, 30 samples of feeds and water samples along with 60 samples of liver and muscle have been examined. The results showed that the feed was indicated to Cd between 0.009-0.202 mg / kg and in the water between 0.0068-0.0096 mg / l. Cd in the livers and muscles was then positively contaminated by Cd between 0.438-0.565 mg / kg and the levels was significantly different (p <0.05) among organs. A strong positive correlation was found between Cd and water levels in liver (r = 0.896). In contrast, a weaker positive correlation (r = 0.596) was found in the water and muscle. In conclusion, Cd contamination in feed and water correlated positively with the increasing amount of the cadmium level in organs.
Determining Image Opacity in Broiler Respiratory Radiographic Using ImageJ and Ansel Adam’s Zone System Ikhwan Wirahadikesuma; Koekoeh Santoso; Hera Maheshwari; Akhiruddin Maddu
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 4 No. 1, JANUARY 2020
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v4i1.8431

Abstract

The diagnosis of diseases of the respiratory, lung, and air sacs generally uses radiographic images by radiologists. Therefore, the results are very subjective, causing differences in the interpretation and diagnosis among different radiologists. A radiographic image reading needs to be made in the form of a simple, fast, and accurate algorithm. The study aimed to reduce subjectivity and be easily carried out by radiology medical personnel, especially veterinarians. This study carried out density measurements by image processing using ImageJ software on 14 radiographic images of broiler chickens. Furthermore, the density value is associated with the Ansel Adam's - grayscale system to determine the opacity of respiratory tract tissues/organs, which were previously inhaled by one of them with chitosan-iopamidol nanoparticles using a nebulizer. The results of density measurements for the category of opacity in radiographic images are that seven spot areas lead brighter (radiopaque) only in chickens that are inhaled mist maker of chitosan-iopamidol nanoparticles. Then the determination of the value range is obtained average value on two ventrodorsal radiographic images, which are inhaled by mist maker of chitosan-iopamidol nanoparticles and chitosan nanoparticle compressor. The conclusion of this study was only in chickens that were infested with mist maker chitosan-iopamidol, whose radiographic image had a radiopaque spot and middle-value area. 
Physiological Status of Stunned and Non-Stunned Broilers: Blood Profile, and Brain Histopathology Aryani Sismin Satyaningtijas; Niken Ulupi; Yulvian Sani; Koekoeh Santoso; Elok Budi Retnani; Hera Maheshwari; Desrayni Hanadhita; Jefri Naldi; Irwan Oktoni; David Anwar; Ainul Khadija Saleema
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 4 No. 2, JULY 2020
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v4i2.10501

Abstract

Stunning method before slaughtering is considered more animal welfare compared to a non-stunning method and also reduces stress levels in broilers. As for the bleed-out efficiency, testing was done indirectly by blood volume measurement which consists of erythrocyte count, leucocyte count, hemoglobin levels, hematocrit values, and erythrocyte index in broilers.   Brain histopathology was also carried out to see the effect of electrical stunning towards the brain tissues.  The stunning method used is electrical stunning via water bath with the capacity of 146 mAh.  The results showed that the stunning method before slaughtering caused smaller number of erythrocytes and hematocrit values, and the brain histopathology showed that stunning causes encephalopathy.
AKTIVITAS ESTROGENIK EKSTRAK ETANOL 70% HERBA KEMANGI (Ocimum americanum L.) PADA TIKUS PUTIH BETINA (Rattus norvegicus) PREMENOPAUSE E.Mulyati Effendi; Hera Maheshwari Hera; Mega Listya M.I
FITOFARMAKA: Jurnal Ilmiah Farmasi Vol 5, No 1 (2015): FITOFARMAKA
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (362.062 KB) | DOI: 10.33751/jf.v5i1.190

Abstract

Ocimum americanum L.(Lamiaceae) dikenal sebagai Kemangi di Indonesia,merupakan tanaman semak dengan bau aromatik yang kuat. Bagian daun dan akar secaratradisional digunakan untuk berbagai pengobatan. Tujuan penelitian ini adalah untukmengetahui aktivitas estrogenik ekstrak etanol 70% herba kemangi (Ocimum americanum L.) pada tikus putih betina (Rattus norvegicus) pre-menopause. Sebanyak 20 tikus putih betinadibagi menjadi 5 kelompok perlakuan, masing-masing diberi perlakuan dengan etinilestradiol (kontrol positif), CMC-Na 1% (kontrol negatif), ekstrak etanol 70% dosis I, II dan II(0,8g/200gBB, 1,6g/200gBB, 3,2g/200gBB). Siklus estrus, vaskularisasi ovarium dan uterusdiamati untuk mengetahui efek dari masing-masing perlakuan. Perlakuan dosis 0,8g/200g BBdapat memperpanjang siklus estrus, meningkatkan vaskularisasi dan bobot ovariumdibandingkan dengan kontrol negatif dan setara dengan etinil estradiol (910mg/200gBB).
Co-Authors . Herdis . Safrida . Yulnawati A. Suprayogi A. Wijaya A.S. Satyaningtijas ACHMAD FARAJALLAH Agik Suprayogi Agus Wijaya Ainul Khadija Saleema Akhiruddin Maddu Amelia Hana Amelia Ramadhani Anshar Anak Agung Gede Sugianthara Anak Agung Istri Sri Wiadnyani Andhika Yudha Prawira Andre, Daniel Latief Andriyanto Andriyanto Arief Boediono Asmarani Kusumawati Bambang Kiranadi Bambang Kiranadi Bambang Kiranadi Bambang Purwantara Claude Mona Airin D. R. Ekastuti Damhuri Damhuri Damiana Rita Ekastuti Danang Dwi Cahyadi David Anwar Dawanto, Jirfan Debby Jacqualine Jochebed Rayer Desrayni Hanadhita Desrayni Hanadhita Desrayni Hanadhita Diah Nugrahani Pristihadi Diani, Yusias Hikmat Dirwan Rahman Divo Jondriatno Divo Jondriatno Dondin Sajuthi Dwi Nurhidayah Zubaidah E Mulyati Effendi, E Mulyati Ekowati Handharyani Elok Budi Retnani ERIC HAYES Ernes Andhesfa Etih Sudarnika Eva Harlina Evi Juliati Evi Juliati Gani F. B. Harlimawan Faisal Tanjung Fitri, Arni Diana Fredi Kurniawan Gandasari, Ira Agustina Dewi Ganjar Maulana Nugraha Ganjar Maulana Nugraha Gilang Kala Maulana, Gilang Kala Hadi S Alikodra Hardiansyah, Muhamad Evans Hendry, Altaff heny Nitbani Herdis - Huda Salahuddin Darusman Huda Salahudin Darusman Huda Shalahudin Darusman Husna Husna, Mar’atul Husna, Rina Siti Nurul I wayan Teguh Wibawan ikhwan wirahadikesuma Ikhwan Wirahadikesuma Irfan Nurhidayat, Irfan Irwan Oktoni Isdoni Bustaman Jefri Naldi Julianto . Juliati Gani, Evi Juliati, Evi Jumadin, La Kasiyati Kasiati koekoeh santoso Kusdiantoro Mohamad Kusuma, Rini Anjarwati La Jumadin Lili Darlian Listya M.I, Mega LUTHFIRALDA SJAHFIRDI Luthfiralda Sjahfirji Luthfiralda Syahfirdi Maneewong, Sattabongkoch Mar’atul Husna Maria Fatima Palupi Maria Fatima Palupi Maria Fatima Palupi Maria Fatima Palupi Maria Prihtamala Omega Maryce Agusthinus Walukou Mega Listya M.I Meta Levi Kurnia Mirnawati Bachrum Sudarwanto Mokhamad Fakhrul Ulum, Mokhamad Mozes R. Tolihere MUHAMMAD ADIB MUSTOFA Muhammad Darjat Darulfalah Muhammad Darjat Darulfalah Muhammad Gunawan Muhammad Rizal Muhammad Zhaahir Mulyati Effendi MULYATI EFFENDI Nambut, Yohanes Baptisto Nastiti Kusomorini Nastiti Kusomorini NASTITI KUSUMORINI NASTITI KUSUMORINI Nastiti Kusumorini Niken Ulupi Nikki Aldi Nikki Aldi, Nikki Nugroho, Setyo Widi Nuri Ardiani Ong Huey, Lynette P. Achmadi Perdhana, Ika Satya Permana, Nadine Hanifa Pudji Achmadi Pudji Astuti Pudji Astuti Pudji Astuti Pudji Astuti PUDJI ASTUTI Purnomo, Rachmatullah Hadi Putri Indah Ningtias Putri Krida Gita P. Putri Krida Gita P. Putri, Fatimatus Sa’diyah Putri, Maritrana R. Tarigan Rachmatullah Hadi Purnomo Rahma Anisa Rahman, Muhammad Luthfi Reviany Widjajakusuma Reviany Widjajakusuma Reviany Widjajakusuma Ridi Arif Rina Siti Nurul Husna Risa Tiuria Rivany Widjajakusuma Rosdianto, Aziiz Mardanarian Rosdianto, Aziiz Mardanarian Rudramurti, Win Satya Safrida Safrida Sandra Hapsari Sandra Hapsari Santoso, Hera Sarmin - Sasmita, Annissa Nuridfi Satvika, Fadhila Siagian, Forman Erwin Slamet Widiyanto Srihadi Agungpriyono Sumiaty Aiba Supratikno . Tarigan, Ronald Teguh Budipitojo Trini Suryowati, Trini Tuty Laswardi Yusuf Umi Cahyaningsih Utami, Dian Maulia W. Manalu Wasmen Manalu Wisnugroho Agung Pribadi Wisnugroho Agung Pribadi Yan, Teo Qin YENI KEZIA BEKALANI Yulnawati - Yulvian Sani Yusfiati Yusfiati Zora, Nelda Fliza