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Journal : Ovozoa: Journal of Animal Reproduction

Porang (Amorphophallus oncophyllus) tuber extract improved the histopathological features of diabetic rat (Rattus norvegicus) testicles Dwi Puspa Sukma Viranda; Rochmah Kurnijasanti; Kadek Rachmawati; Iwan Sahrial Hamid; Pudji Srianto; Hani Plumeriastuti; Lita Rakhma Yustinasari
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.47-57

Abstract

This study aims to determine the effect of porang (Amorphophallus oncophyllus) tuber extract on the histopathological features of the testicle of alloxan induced rats (Rattus norvegicus). Thirty male rats were randomly divided into six groups. Rats in group C- were injected with distilled water, while rats in groups C+, T0, T1, T2, and T3 were injected with 120 mg/kg bw alloxan. Blood glucose was measured three days after alloxan injection. Rats in groups C- and C+ were then administered with 1% Na-CMC, whereas rats in groups T0, T1, T2, and T3 were then administered with 45mg/kg bw metformin, and 100, 200, and 400 mg/kg bw of porang tuber extract, respectively. All solution were given orally once every day for 14 days. Spermatogenic activity was assessed using the Johnsen scoring system and analyzed by Kruskal-Wallis, followed by the Mann-Whitney test. The diameter and epithelial thickness of the seminiferous tubule were measured using image raster software and analyzed using Anova followed by Duncan's test. The results showed that spermatogenesis score, diameter, and epithelial thickness of seminiferous tubule of group C+ were smaller (p <0.05) than group C-. Spermatogenesis scores of groups T0, T1, T2, and T3 were higher (p <0.05) than group C+. Seminiferous tubule diameter and epithelial thickness in groups T0, T1, and T2 were greater (p <0.05) than group C+. It could be concluded that porang tuber extract at a dose of 200 mg/kg bw improved the spermatogenesis score, diameter seminiferous tubule, and thickness of the epithelium of diabetic rats.
Comparison of reproductive performance of Ongole cross and Ongole-Limousin cross in Bekri district, Central Lampung regency, Lampung province, Indonesia Sukma, Puspa Permata; Ismudiono Ismudiono; Sri Mulyati; Pudji Srianto; Sri Pantja Madyawati; Ira Sari Yudaniayanti; Abdullah Hasib
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.9-17

Abstract

This study aims to compare the reproductive performance of the Ongole cross (PO) and Ongole-Limousin cross (Limpo) on smallholder farmer in Bekri district, Central Lampung regency, Lampung province, a Indonesia. This study used 30 PO cows, 30 Limpo F1 (first filial) cows and 30 Limpo F2 cows. Sample cows had at least two parities and had never had a history of reproductive disorders. This study used primary and secondary data related to sample cows. Primary data was obtained from interviews with farmers and secondary data was obtained from the Department of Plantations, Livestock and Fisheries of Central Lampung regency, and inseminator recording. Data were described descriptively and analyzed using a one-way Analysis of Variance in Statistical Product and Service Solution (SPSS) version 23. The result showed that the services per conception of Limpo F2 cows was higher (p <0.05) than for PO and Limpo F1 cows. Days open and calving interval for Limpo cows (F1 and F2) were longer (p <0.05) than for PO cows. Days open and calving interval for Limpo F1 cows were not significantly different (p >0.05) than for Limpo F2 cows. Reproductive performance of PO cows was better compared to Limpo F1 and Limpo F2 cows. However, there was a data discrepancy between reproductive performance and data on rearing systems, feed quality, and signs of estrus observed by farmers. It could be concluded that PO cows had better reproductive performance than Limpo cows.
Addition of basil leaf (Ocimum basicilum L.) extract to AndroMed® extender improved the quality of spermatozoa of frozen thawed Simmental bull semen Aditya Irawan; Fajar Ramadhan Subiyantoro; Suherni Susilowati; Tri Wahyu Suprayogi; Sri Pantja Madyawati; Pudji Srianto; Nove Hidajati; Eduardus Bimo Aksono Herupradoto; Kadek Rachmawati
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 3 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i3.2024.161-170

Abstract

This study aims to determine the best dose of basil leaf extract to add to AndroMed® semen extender to improve spermatozoa quality after freezing and thawing. This study used healthy, normal four-year-old Simmental bulls with good libido. The sample used was fresh semen from Simmental bull collected using an artificial vagina. This study used a completely randomized design (CRD) with three treatments and six replicates. Ejaculate that met the motility qualification of more than 70%, was divided into three groups, each extended in AndroMed® without the addition of basil leaf extract (T0), with the addition of 0.1% basil leaf extract (T1) and with the addition of 0.3% basil leaf extract (T2). Straw containing semen with 30 million spermatozoa/straw was processed using an IceCube automatic freezer (Minitube, Germany). The results showed that spermatozoa motility, viability, and spermatozoa plasma membrane integrity in the T2 group were higher (p <0.05) than those in the T0 and T1 groups, while spermatozoa morphological abnormalities in the T2 group were lower (p <0.05) than those in the T0 and T1 groups. There were no significant differences (p >0.05) in these parameters between groups T0 and T1. It could be concluded that the addition of 0.3% basil leaf extract to the AndroMed® extender could improve the quality of the semen of Simmental bulls after thawing.
Estrus quality, non-return rate and conception rate in the incidence of repeat breeding in dairy cow after 2%povidone iodine therapy combined with prostaglandin F2α at KUD Tani Wilis Tulungagung Zahrorona, Amira Halimah; Sri Pantja Madyawati; Pudji Srianto; Hermin Ratnani; Sunaryo Hadi Warsito; Sri Hidanah
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 1 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i1.2025.46-51

Abstract

Repeat breeding is a condition in which cows fail to conceive after being bred three or more times. This study aims to determine the quality of estrus, non-return rate (NRR) and conception rates obtained after cows were treated with 2% povidone iodine. The study involved 30 Holstein Friesian cows, each having calved three times and experiencing repeat breeding. Cows in estrus were treated with 10 mL of 2% povidone iodine intrauterinely, followed by an intramuscular injection of 2 mL (250 mg/head) of prostaglandin F2α (PGF2α, Luteosyl) five days later. Estrus quality was observed by examining changes in vulvar mucosa color, mucus discharge, and vulvar temperature. Pregnancy diagnosis was made at 60 days. Results showed that 43.3% of cows had estrus quality score 3 (cows showed dark red vulvar mucosa, transparent mucus, temperature 38.9 ± 0.5 ºC), 23.3% had score 2 (cows showed reddish vulvar mucosa, some cloudy mucus, temperature 38.7 ± 1.0 ºC), and 33.3% had score 1 (cows showed pink vulvar mucosa, cloudy mucus, temperature 38.6 ± 0.5 ºC). Of the 30 cows, 20 had a non-return rate (NRR) of 66.7%. Based on these results, it can be concluded that 2% povidone iodine has a positive effect on estrus quality, NRR, and conception rates in dairy cows experiencing repeat breeding.
Factors associated with repeat breeding in Holstein Friesian cows at the Tani Wilis Village Cooperative Unit, Sendang District, Tulungagung Regency, Indonesia Dimas Kunto Satrio; M’ Izi Kumala Lazuardi Sultoni; Tasya Apritalia Putri; Soeharsono Soeharsono; Dadik Rahardjo; Djoko Legowo; Gandul Atik Yuliani; Iwan Sahrial Hamid; Mohammad Anam Al-Arif; Sunaryo Hadi Warsito; Pudji Srianto; Sri Pantja Madyawati; Tita Damayanti Lestari; Wurlina Wurlina
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 2 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i2.2025.67-75

Abstract

This study aimed to evaluate the association of factors such as nutrition, thermal humidity index (THI), age, and lactation period with the occurrence of repeat breeding of dairy cows. The sample consisted of Holstein Friesian cows aged 1-11 years, with lactation periods ranging from 1 to 7 months, currently in lactation, and experiencing repeat breeding (inseminated three or more times without conception, despite exhibiting normal estrous cycles and intervals). The results showed that feeding practices by farmers did not significantly differ (p >0.05) in relation to the incidence of repeat breeding. THI values recorded in several barns also showed no significant differences (p >0.05), with the cows experiencing light to moderate heat stress. Similarly, the frequency of artificial insemination and the estrous cycle intervals remained within the normal range. The highest incidence of repeat breeding was observed in cows aged 2.5 to 5.5 years, accounting for 78.13%, and during lactation periods of 1-3 months, accounting for 81.26%. It can be concluded that there were no significant differences in the provision of forage and leguminous feed between normal cows and those with repeat breeding, and the crude protein content of forage did not influence its occurrence. Additionally, there was no indirect relationship between THI and repeat breeding. However, the incidence of repeat breeding tended to increase in cows aged 3-4 years, and during the second and third lactation periods.
CONCEPTION RATE DAN SERVICE PER CONCEPTION PADA SAPI PERANAKAN LIMOUSIN DAN SAPI PERANAKAN ONGOLE HASIL IB DI KECAMATAN PILANGKENCENG KABUPATEN MADIUN Bhaskara, Alfiutha Propha; Suprayogi, Tri Wahyu; Darsono, Roesno; Srianto, Pudji; Hariadi, Mas'ud; Sardjito, Trilas
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (303.328 KB) | DOI: 10.20473/ovz.v7i2.2018.152-155

Abstract

The study was conducted to know conception rate and service per conception of Limousin Cross breed and Ongole Cross Breed in Pilangkenceng, Madiun. Data were collected for the periode of Oktober 2016 to February 2017 from inseminators in Pilangkenceng district. The data were taken by primary and secondary. Primary data retrieval were done by direct observation, which includes several variables, where the variables include: the identity of the breeder, feeding and drinking. The secondary data were obtained by recording the farmer's artificial insemination card. All data was analyzed to find a percentage of conception rate (CR) and find number of service per conception (S/C). Then S/C were analyzed with T-test adn CR were analyzed with chi-square test. The results of this research showed that the service per conception's average of limousin cross breed and ongole cross breed is 1,61 and 1,72. The average of conception rate limousin cross breed and ongole cross breed is 53% % and 59 %. The result showed no significant difference (p>0,05) on CR and S/C between limousin cross breed and ongole cross breed. The conclusion of this research showed that service per conception and conception rate of ongole cross breed in Pilengkenceng Madiun is better than limousin cross breed but there's no significant difference (p>0,05).
UJI BERBAGAI ALAT DETEKSI BIRAHI TERHADAP ANGKA KEBUNTINGAN PADA SAPI PERAH DI KOPERASI TUNAS SETIA BARU KABUPATEN PASURUAN Andriyanti, Silvia Rani; Hariadi, Mas'ud; Darsono, Roesno; Srianto, Pudji; wurlina, wurlina; Restiadi, Tjuk Imam
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (401.453 KB) | DOI: 10.20473/ovz.v7i2.2018.156-159

Abstract

This research aims to determine the use of various estrus detection devices before artificial insemination as measured by pregnancy rate. The researcher used a sample of 60 cows were used in this research which is divided into four groups as a control and treatment groups (Thermopin®, Hauptner and Draminski groups), and each cow was detected for the sign of estrus by three devices that were Thermopin®, Hauptner and Draminski prion when doing an artificial insemination. The cows were observed to 45 days after artificial insemination for observing pregnancy. This research method is the exploration scope to prove the effect of various estrus detection tool against pregnancy rates. The result of ANOVA test, there is the effect of using a various estrus detection devices against pregnancy rates. Samples tested by Draminski with average (1.53±0.51) resulted in a 53,3% pregnancy rate, the samples were tested with Hauptner with average (1.67±0,48) resulted in pregnancy rate 66,7%. Without treatment or control, sample with an average of (1.67±0,48) resulted in a  73,3% pregnancy rate. While samples were tested with Thermopin® with average (2.00±0,00) resulted in a 100% pregnancy rate. In conclusion, Thermopin® the best detection tool than other estrus detection devices.
KADAR MILK UREA NITROGEN (MUN) PADA SAPI PERANAKAN FRIESIAN HOLSTEIN (PFH) BERDASARKAN CALVING INTERVAL YANG BERBEDA Pratama, Hanif Sabekti; Mustofa, Imam; Lokapirnasari, Widya Paramita; Srianto, Pudji; Rimayanti, Rimayanti; Utama, Suzanita
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 1 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.387 KB) | DOI: 10.20473/ovz.v8i1.2019.1-4

Abstract

Milk urea nitrogen (MUN) analys may be used as a signal potential problem in feeding program. The aim of this research was to know levels of milk urea nitrogen with different calving interval. This research was using purposive sampling in KUD Tani Wilis, Sendang, Tulungagung. The sample was obtained from breeder as much as 16 samples, grouped into 2 groups, group 1 calving interval ≤370 and group 2 calving interval ≥450. This samples tested in Surabaya laboratory of health (Balai Besar Laboratorium Kesehatan Surabaya). The result showed that MUN not signifcanly different with different calving interval (p>0,05). The conclusion of this research was MUN did not related with different calving interval.
PERBANDINGAN BODY CONDITION SCORE, SERVICES PER CONCEPTION, CALVING INTERVAL, DAN DAYS OPEN SAPI FRIESIAN HOLSTEIN (FH) DI KEMITRAAN GREENFIELD DENGAN KUD TANI WILIS SENDANG Cahya P., Aghid; Mulyati, Sri; Suwarno, Suwarno; Mustofa, Imam; Srianto, Pudji; Utomo, Budi
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.497 KB) | DOI: 10.20473/ovz.v8i2.2019.149-153

Abstract

The purpose of this study was to determine the comparison of Body Condition Score, Services Per Conception, Calving Interval, and Days Open cow friesian holstein (FH) in Greenfield Partnership and Tani Wilis Sendang Cooperation. The material used in this research is dairy cattle that have been inseminated in Greenfield Partnership and KUD Tani Wilis with secondary data retrieval of reproduction notes during IB until partus and quisioner distributed by farmers and inseminators. The exploratory survey method used is secondary data obtained from the record reproduction inseminator. The observed variables BCS, S/C, CI and DO . The data was processed using descriptive analysis to get a number BCS, S/C, CI and DO in Greenfield Partnership and Tani Wilis Sendang Cooperation. The results showed that the average value of BCS at Greenfield Partnership is 4.86 and at Tani Wilis Cooperation is 4.74. The average value of S/C in Greenfield Partnership is 4.36 and at Tani Wilis Sendang Cooperation is 2.06. The average value CI at Greenfield Partnership is 463.00 and at Tani Wilis Sendang is 414.40. The average value DO at Greenfield Partnership is 64.84 and at Tani Wilis Sendang is 88.00. It can be concluded that the comparison of BCS in both places is not significantly different while for the S/C of Tani WilisSendang Cooperation is better than Greenfield Partnership, the CalvingInterval in both places is still less good because it is far from the standard CI value, and for the Days Open Greenfield partnership is better thanTaniWilisSendang Cooperation.
PENGARUH pH LENDIR MUKOSA VAGINA SAAT BIRAHI TERHADAP PERSENTASE KEBUNTINGAN (Conception Rate) PADA SAPI PERAH DI KUD TANI WILIS KABUPATEN TULUNGAGUNG DAN KSU TUNAS SETIA BARU KABUPATEN PASURUAN Rizki, Andaru; Srianto, Pudji; Suprihati, Endang; Sardjito, Trilas; Ismudiono, Ismudiono; Al Arif, Mohammad Anam
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.312 KB) | DOI: 10.20473/ovz.v8i2.2019.154-158

Abstract

The purpose of this research is to determine the effect of mucos acidity of vaginal mucous when estrus towards conception rate at KUD Tani Wilis Tulungagung District and KSU Tunas Setia Baru Pasuruan District. Measured samples were taken from 30 dairy cattles in each KUD. Mucos acidity of vaginal mucous were obtained by using a digital acidity meter before insemination was done on dairy cattle. After measuring the acidity from mucosal vagina mucous, artificial insemination has done on estrus dairy cattle. On the 60th day after insemination, rectal palpation was done to check the conception rate. Research result from 30 dairy cattle on each KUD showed that mucos acidity of vaginal mucous has signifficant effect towards conception rate. Mucos acidity of vaginal mucous range 6.5-8.5 with the highest range of conception rate in KSU Tunas Setia Baru Pasuruan District 7.5-7.7 and in KUD Tani Wilis Tulungagung District with the highest range of conception range 7.2-7.4 and 7.5-7.7.
Co-Authors . Kholik Abdul Samik, Abdul Abdullah Hasib Aditya Irawan Aftabuddin Rz, Muhammad Agustono, Bodhi Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Aldin Akbar Rahmatullah Ali Saifudin Andriyanti, Silvia Rani Azwani, Nabila Bambang Poernomo Berliana, Erprinanda Galuh Bhaskara, Alfiutha Propha Boedi Setiawan Brillianti, Febby Fairy Budi Utomo BUDI UTOMO Budiarto Budiarto Budiarto Cahya P., Aghid Cindy Ercha Aulia Putri Dadik Rahardjo Dadik Rahardjo, Dadik Dadik Raharjo Dadik Raharjo Daniel Leonardo Darsono, Roesno Deskayanti, Asih Dhesy Kartikasari Dian Ayu Permatasari Dian Ayu Permatasari Dikman, Dicky Mohammad Dimas Kunto Satrio Djoko Legowo Dwi Puspa Sukma Viranda Emy Koestanti Sabdoningrum Endang Suprihati Erlangga, Made Bagus Erma Safitri Erma Safitri ERMA SAFITRI Fajar Ramadhan Subiyantoro Febriano, Muhammad Galang Gandul Atik Yuliani Hani Plumeriastuti Hariadi, Mas'ud Heni Puspitasari Hermin Ratnani Herry Agoes Hermadi IMAM MUSTOFA Imam Mustofa Indah Norma Triana, Indah Norma Ira Sari Yudaniayanti Ismudiono Ismudiono Ismudiono Ismudiono, Ismudiono Iwan Sahrial Hamid Kadek Rachmawati Kartikasari, Dhesy Kholik, Kholik Kurniawan, Muhammad ‘Ahdi Laba Mahaputra Lailiyah, Fahlevi Luqman, Epy Muhammad Masyitoh, Herlina Mohammad Auzaie Afandi Muhammad Aulia Rahman Muhammad Munawaroh Muhammad Rama Imam Saputra Muhammad Yunus Munawaroh, Muhammad M’ Izi Kumala Lazuardi Sultoni Nidom, Chairul Anwar Nove Hidajati Nurhusien Yimer Nuurin Ajrin Karim Praja, Ratih Novita Prastiya, Ragil Angga Pratama, Hanif Sabekti Pristian Devon Martyas Wahyuningrum Rahmawati Rahmawati Rahmawati, Mitha Ardila Rahmawati, Rahmawati Rimayanti Rimayanti Rimayanti Rimayanti Rimayanti Ristiani, Widya Aulia Rizki, Andaru Rochmah Kurnijasanti Saputra, Muhammad Rama Imam Saputro, Amung Logam Seagames Waluyo, Seagames Setiawan Koesdarto Setyadi, Arda Wahyu Sintya Kumalasari Wibowo Sintya Kumalasari Wibowo Soeharsono Soeharsono Soeharsono Soeharsono Sofie Adi, Keren Jayanti Sri Hidanah Sri Mulyati Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Subagio, Muharam Suherni Susilowati Sukma, Puspa Permata Sunarso, Agus Supriyadi Supriyadi Suwarno Suwarno Suzanita Utama Tasya Apritalia Putri Tatik Hernawati Tita Damayanti Lestari Tjuk Imam Restiadi Tri Wahyu Suprayogi Trilas Sardjito Viski Fitri Hendrawan Warsito, Sunaryo Hadi Widjiati w Widjiati Widjiati Widjiati Widjiati, Widjiati Widodo, Oky Setyo Widya Paramita Lokapirnasari Wulandari, Shelly Wurlina, W Yustinasari, Lita Rakhma Zahrorona, Amira Halimah